AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54134

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54134.t1 MTR_2g068760 98.592 71 1 0 1 71 73 143 9.54e-46 143
MS.gene54134.t1 MTR_4g083680 63.636 77 21 2 1 70 75 151 8.98e-26 93.2
MS.gene54134.t1 MTR_4g060950 72.917 48 13 0 1 48 85 132 5.29e-19 76.3
MS.gene54134.t1 MTR_1g095850 72.917 48 13 0 1 48 132 179 4.01e-18 74.7
MS.gene54134.t1 MTR_2g093310 62.500 48 18 0 1 48 93 140 2.35e-17 72.0
MS.gene54134.t1 MTR_3g031660 66.667 51 17 0 1 51 67 117 3.05e-17 71.6
MS.gene54134.t1 MTR_5g083230 73.913 46 12 0 1 46 66 111 3.59e-17 70.9
MS.gene54134.t1 MTR_3g071590 71.739 46 13 0 1 46 68 113 1.10e-16 69.7
MS.gene54134.t1 MTR_3g094690 56.250 48 21 0 1 48 106 153 3.91e-14 64.3
MS.gene54134.t1 MTR_3g094690 56.250 48 21 0 1 48 107 154 3.91e-14 64.3
MS.gene54134.t1 MTR_3g077240 56.250 48 21 0 1 48 70 117 4.27e-13 60.8
MS.gene54134.t1 MTR_5g083960 47.761 67 26 1 1 67 99 156 1.19e-12 59.7
MS.gene54134.t1 MTR_4g105170 49.206 63 29 1 1 63 71 130 2.03e-12 58.9
MS.gene54134.t1 MTR_6g005070 52.941 51 23 1 1 51 69 118 3.62e-12 57.8
MS.gene54134.t1 MTR_7g033800 55.814 43 19 0 1 43 71 113 2.64e-11 55.8
MS.gene54134.t1 MTR_7g096530 58.140 43 18 0 1 43 75 117 3.73e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54134.t1 AT1G16530 62.069 58 18 2 1 57 75 129 1.42e-19 77.4
MS.gene54134.t1 AT1G31320 85.417 48 7 0 1 48 73 120 1.69e-17 72.0
MS.gene54134.t1 AT2G30130 63.158 57 19 1 1 55 68 124 2.09e-16 69.7
MS.gene54134.t1 AT1G07900 64.583 48 17 0 1 48 93 140 8.08e-16 68.2
MS.gene54134.t1 AT2G28500 62.000 50 19 0 1 50 115 164 7.07e-15 66.2
MS.gene54134.t1 AT5G63090 60.870 46 18 0 1 46 71 116 2.92e-13 61.2
MS.gene54134.t1 AT5G63090 60.870 46 18 0 1 46 71 116 2.92e-13 61.2
MS.gene54134.t1 AT5G63090 60.870 46 18 0 1 46 71 116 2.92e-13 61.2
MS.gene54134.t1 AT5G63090 60.870 46 18 0 1 46 71 116 2.92e-13 61.2
MS.gene54134.t1 AT2G30340 51.111 45 22 0 1 45 112 156 3.18e-12 59.7
MS.gene54134.t1 AT2G30340 51.111 45 22 0 1 45 113 157 3.20e-12 59.7

Find 15 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTGTGCTTGAGCTAAT+TGG 0.150837 2.2:-23416845 None:intergenic
TGCTTGTGCTTGAGCTAATT+GGG 0.350545 2.2:-23416844 None:intergenic
AAGAAAAGAACCTGCTTGAT+TGG 0.406684 2.2:-23416953 None:intergenic
ATGAAGCAAATGCAAGAGTA+AGG 0.428990 2.2:+23416757 MS.gene54134:CDS
GATGATGATCAGATGAGAAT+TGG 0.476334 2.2:-23416890 None:intergenic
AGAGTAAGGGATCCTGTGTA+TGG 0.521523 2.2:+23416771 MS.gene54134:CDS
TGAAGCAAATGCAAGAGTAA+GGG 0.570272 2.2:+23416758 MS.gene54134:CDS
AAAATGTGTACCAATCAAGC+AGG 0.584103 2.2:+23416943 MS.gene54134:CDS
GGATCCTGTGTATGGATGTG+TGG 0.591101 2.2:+23416779 MS.gene54134:CDS
GATCCTGTGTATGGATGTGT+GGG 0.634230 2.2:+23416780 MS.gene54134:CDS
ATTAGCTCAAGCACAAGCAG+AGG 0.647477 2.2:+23416848 MS.gene54134:CDS
GATGGTGGTGACGAATTCGA+TGG 0.671990 2.2:-23416918 None:intergenic
ATCTACTTGTTGCTGCAGAG+AGG 0.672706 2.2:-23416811 None:intergenic
GGTGGTGACGAATTCGATGG+TGG 0.676502 2.2:-23416915 None:intergenic
GCTCCCACACATCCATACAC+AGG 0.680929 2.2:-23416783 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGAAGCAAATGCAAGAGTA+AGG + chr2.2:23416757-23416776 MS.gene54134:CDS 35.0%
GATGATGATCAGATGAGAAT+TGG - chr2.2:23416893-23416912 None:intergenic 35.0%
TGAAGCAAATGCAAGAGTAA+GGG + chr2.2:23416758-23416777 MS.gene54134:CDS 35.0%
! GATTGGTACACATTTTCTGA+TGG - chr2.2:23416939-23416958 None:intergenic 35.0%
TGCTTGTGCTTGAGCTAATT+GGG - chr2.2:23416847-23416866 None:intergenic 40.0%
! TGGTACACATTTTCTGATGG+TGG - chr2.2:23416936-23416955 None:intergenic 40.0%
AGAGTAAGGGATCCTGTGTA+TGG + chr2.2:23416771-23416790 MS.gene54134:CDS 45.0%
ATTAGCTCAAGCACAAGCAG+AGG + chr2.2:23416848-23416867 MS.gene54134:CDS 45.0%
CTGCTTGTGCTTGAGCTAAT+TGG - chr2.2:23416848-23416867 None:intergenic 45.0%
GATCCTGTGTATGGATGTGT+GGG + chr2.2:23416780-23416799 MS.gene54134:CDS 45.0%
! ATCTACTTGTTGCTGCAGAG+AGG - chr2.2:23416814-23416833 None:intergenic 45.0%
GGATCCTGTGTATGGATGTG+TGG + chr2.2:23416779-23416798 MS.gene54134:CDS 50.0%
! GATGGTGGTGACGAATTCGA+TGG - chr2.2:23416921-23416940 None:intergenic 50.0%
GCTCCCACACATCCATACAC+AGG - chr2.2:23416786-23416805 None:intergenic 55.0%
! GGTGGTGACGAATTCGATGG+TGG - chr2.2:23416918-23416937 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 23416750 23416964 23416750 ID=MS.gene54134
chr2.2 mRNA 23416750 23416964 23416750 ID=MS.gene54134.t1;Parent=MS.gene54134
chr2.2 exon 23416750 23416964 23416750 ID=MS.gene54134.t1.exon1;Parent=MS.gene54134.t1
chr2.2 CDS 23416750 23416964 23416750 ID=cds.MS.gene54134.t1;Parent=MS.gene54134.t1
Gene Sequence

>MS.gene54134

ATGGTATATGAAGCAAATGCAAGAGTAAGGGATCCTGTGTATGGATGTGTGGGAGCTATATCCTCTCTGCAGCAACAAGTAGATGTGCTACAAACCCAATTAGCTCAAGCACAAGCAGAGGTTGTGCATATGAAGATGCACCAATTCTCATCTGATCATCATCAACCACCATCGAATTCGTCACCACCATCAGAAAATGTGTACCAATCAAGCAG
Protein sequence

>MS.gene54134.t1

MVYEANARVRDPVYGCVGAISSLQQQVDVLQTQLAQAQAEVVHMKMHQFSSDHHQPPSNSSPPSENVYQSS