Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54190.t1 | XP_003611394.3 | 93.7 | 175 | 11 | 0 | 1 | 175 | 1 | 175 | 2.20E-87 | 331.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54190.t1 | Q84R94 | 42.6 | 162 | 75 | 4 | 17 | 161 | 54 | 214 | 3.0e-21 | 103.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54190.t1 | A0A396HMK7 | 93.7 | 175 | 11 | 0 | 1 | 175 | 1 | 175 | 1.6e-87 | 331.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene54190.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54190.t1 | MTR_5g013530 | 87.640 | 178 | 16 | 2 | 1 | 177 | 1 | 173 | 1.03e-113 | 320 |
| MS.gene54190.t1 | MTR_5g013520 | 92.466 | 146 | 11 | 0 | 30 | 175 | 1 | 146 | 1.06e-98 | 281 |
| MS.gene54190.t1 | MTR_5g013530 | 86.667 | 150 | 14 | 2 | 29 | 177 | 7 | 151 | 1.54e-91 | 263 |
| MS.gene54190.t1 | MTR_5g013515 | 73.913 | 138 | 28 | 3 | 31 | 161 | 1 | 137 | 3.47e-68 | 204 |
| MS.gene54190.t1 | MTR_2g042900 | 44.172 | 163 | 44 | 6 | 25 | 156 | 99 | 245 | 1.60e-32 | 117 |
| MS.gene54190.t1 | MTR_4g124960 | 35.849 | 159 | 72 | 6 | 5 | 162 | 73 | 202 | 4.24e-19 | 80.9 |
| MS.gene54190.t1 | MTR_2g042900 | 33.533 | 167 | 41 | 5 | 25 | 130 | 99 | 256 | 1.34e-17 | 77.8 |
| MS.gene54190.t1 | MTR_4g124960 | 35.878 | 131 | 55 | 5 | 5 | 135 | 73 | 174 | 2.15e-15 | 70.5 |
| MS.gene54190.t1 | MTR_4g124950 | 34.783 | 138 | 64 | 5 | 5 | 141 | 7 | 119 | 2.23e-13 | 63.9 |
| MS.gene54190.t1 | MTR_8g021380 | 31.538 | 130 | 79 | 3 | 19 | 138 | 79 | 208 | 2.84e-13 | 65.9 |
| MS.gene54190.t1 | MTR_2g044910 | 28.571 | 112 | 65 | 1 | 44 | 140 | 15 | 126 | 4.16e-13 | 64.3 |
| MS.gene54190.t1 | MTR_2g044910 | 28.571 | 112 | 65 | 1 | 44 | 140 | 58 | 169 | 7.85e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54190.t1 | AT1G19180 | 41.667 | 132 | 68 | 3 | 37 | 159 | 53 | 184 | 1.54e-25 | 97.4 |
| MS.gene54190.t1 | AT1G19180 | 41.045 | 134 | 70 | 3 | 37 | 161 | 119 | 252 | 4.97e-25 | 97.8 |
| MS.gene54190.t1 | AT1G19180 | 41.045 | 134 | 70 | 3 | 37 | 161 | 119 | 252 | 4.97e-25 | 97.8 |
| MS.gene54190.t1 | AT1G74950 | 41.304 | 138 | 62 | 4 | 37 | 157 | 112 | 247 | 5.91e-24 | 94.7 |
| MS.gene54190.t1 | AT1G74950 | 42.727 | 110 | 47 | 2 | 37 | 130 | 112 | 221 | 4.56e-19 | 81.3 |
| MS.gene54190.t1 | AT5G20900 | 31.973 | 147 | 78 | 4 | 24 | 148 | 28 | 174 | 1.96e-15 | 70.9 |
| MS.gene54190.t1 | AT1G72450 | 36.364 | 143 | 69 | 4 | 39 | 165 | 101 | 237 | 5.06e-14 | 68.6 |
| MS.gene54190.t1 | AT1G17380 | 34.426 | 122 | 66 | 2 | 33 | 140 | 58 | 179 | 1.61e-13 | 66.6 |
| MS.gene54190.t1 | AT1G17380 | 34.426 | 122 | 66 | 2 | 33 | 140 | 87 | 208 | 2.81e-13 | 66.6 |
Find 36 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTACAAGAGATTCCTATTT+TGG | 0.046444 | 5.1:+6423116 | MS.gene54190:CDS |
| CCATCTATACCTGTTATTTA+TGG | 0.164541 | 5.1:+6423515 | MS.gene54190:CDS |
| CTTGGATTGCTTGTTTGATA+TGG | 0.217861 | 5.1:-6424157 | None:intergenic |
| ATTGGTTTATTAATGCTTCT+TGG | 0.268448 | 5.1:-6424175 | None:intergenic |
| AGCTTCACTTCATCGGTTCT+TGG | 0.292747 | 5.1:+6423656 | MS.gene54190:CDS |
| CATGTCATGGATTCATCAAT+TGG | 0.320863 | 5.1:-6424193 | None:intergenic |
| CCAAAATAGGAATCTCTTGT+AGG | 0.331403 | 5.1:-6423116 | None:intergenic |
| ATCCATGACATGGCTCTCTT+TGG | 0.358156 | 5.1:+6424204 | MS.gene54190:CDS |
| TACCTGGAGTTTCCCAAAAT+AGG | 0.395618 | 5.1:-6423129 | None:intergenic |
| GGAAGTTGTTGAAAATTCAT+AGG | 0.405668 | 5.1:-6423084 | None:intergenic |
| GGAAAAGTCCATGATGTCTT+TGG | 0.411671 | 5.1:-6423396 | None:intergenic |
| AGTGAAGCTTTCCTAGTCAT+TGG | 0.425048 | 5.1:-6423642 | None:intergenic |
| TGAAGTACTAGTAATTCCTT+TGG | 0.431697 | 5.1:-6423420 | None:intergenic |
| TTCATCGGTTCTTGGAGAAA+AGG | 0.463618 | 5.1:+6423664 | MS.gene54190:CDS |
| GCTTCTGCAGAATTGCAGCC+AGG | 0.464388 | 5.1:+6424131 | MS.gene54190:intron |
| AGTAAGCATAGTTGTTATTG+TGG | 0.472037 | 5.1:-6423448 | None:intergenic |
| CCATAAATAACAGGTATAGA+TGG | 0.477854 | 5.1:-6423515 | None:intergenic |
| GCTTGTTTGATATGGTGCCC+TGG | 0.478945 | 5.1:-6424149 | None:intergenic |
| AGTACTAGTAATTCCTTTGG+TGG | 0.499055 | 5.1:-6423417 | None:intergenic |
| GATTTATGTTGTGGTGATTG+TGG | 0.515021 | 5.1:-6424229 | None:intergenic |
| CTAGGAAAGCTTCACTTCAT+CGG | 0.529148 | 5.1:+6423649 | MS.gene54190:CDS |
| CAGGTATAGATGGTACTTGA+GGG | 0.533329 | 5.1:-6423505 | None:intergenic |
| AATGAAGGCTAATATTAAGA+AGG | 0.546955 | 5.1:+6423295 | MS.gene54190:CDS |
| CTACACTCAGATTTATGTTG+TGG | 0.562374 | 5.1:-6424238 | None:intergenic |
| ATCTCTTGTAGGAAGAGATG+AGG | 0.564072 | 5.1:-6423105 | None:intergenic |
| TCTTGGAGAAAAGGAAGGAT+AGG | 0.567398 | 5.1:+6423673 | MS.gene54190:CDS |
| ACAGGTATAGATGGTACTTG+AGG | 0.569787 | 5.1:-6423506 | None:intergenic |
| CTTCTGCAGAATTGCAGCCA+GGG | 0.572555 | 5.1:+6424132 | MS.gene54190:intron |
| TGCCAAAGAGAGCCATGTCA+TGG | 0.583954 | 5.1:-6424206 | None:intergenic |
| TCGGTTCTTGGAGAAAAGGA+AGG | 0.585705 | 5.1:+6423668 | MS.gene54190:CDS |
| ATTCAGATTTACCAATGACT+AGG | 0.608086 | 5.1:+6423631 | MS.gene54190:intron |
| TTAAAGTAACACATGGGTGG+AGG | 0.612419 | 5.1:-6424266 | None:intergenic |
| CAATTGATGAATCCATGACA+TGG | 0.622850 | 5.1:+6424194 | MS.gene54190:CDS |
| AAAACAGTTAAAGTAACACA+TGG | 0.639241 | 5.1:-6424273 | None:intergenic |
| CAGTTAAAGTAACACATGGG+TGG | 0.664883 | 5.1:-6424269 | None:intergenic |
| AAACAGTTAAAGTAACACAT+GGG | 0.695262 | 5.1:-6424272 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAATTTAATGTAAGTT+TGG | + | chr5.1:6423789-6423808 | MS.gene54190:intron | 10.0% |
| !! | AAAAAATTACCATAAATAAC+AGG | - | chr5.1:6423527-6423546 | None:intergenic | 15.0% |
| !!! | TGTTATTTATGGTAATTTTT+TGG | + | chr5.1:6423526-6423545 | MS.gene54190:intron | 15.0% |
| !! | ATTACTAATACTATGATGTT+TGG | + | chr5.1:6424079-6424098 | MS.gene54190:intron | 20.0% |
| !! | TTACTAATACTATGATGTTT+GGG | + | chr5.1:6424080-6424099 | MS.gene54190:intron | 20.0% |
| !!! | AAATGACAATCTTTTATGAT+GGG | + | chr5.1:6423336-6423355 | MS.gene54190:CDS | 20.0% |
| !!! | GTTAATAGATTTCACTTTTT+GGG | + | chr5.1:6423739-6423758 | MS.gene54190:intron | 20.0% |
| !!! | TGTTAATAGATTTCACTTTT+TGG | + | chr5.1:6423738-6423757 | MS.gene54190:intron | 20.0% |
| !!! | TTAATAGATTTCACTTTTTG+GGG | + | chr5.1:6423740-6423759 | MS.gene54190:intron | 20.0% |
| ! | AAACATGTCAAAATCAATCT+TGG | - | chr5.1:6423832-6423851 | None:intergenic | 25.0% |
| ! | AACAAACATATCAGTCAATA+TGG | - | chr5.1:6424108-6424127 | None:intergenic | 25.0% |
| ! | AATGAAGGCTAATATTAAGA+AGG | + | chr5.1:6423295-6423314 | MS.gene54190:CDS | 25.0% |
| ! | CATACTAGTAGTATATAACA+TGG | + | chr5.1:6423227-6423246 | MS.gene54190:intron | 25.0% |
| ! | TACATTCAACTCAATCTTAA+AGG | + | chr5.1:6423988-6424007 | MS.gene54190:intron | 25.0% |
| ! | TGGATATATTAAGTTTGAGA+GGG | + | chr5.1:6423546-6423565 | MS.gene54190:intron | 25.0% |
| ! | TTGGATATATTAAGTTTGAG+AGG | + | chr5.1:6423545-6423564 | MS.gene54190:intron | 25.0% |
| !! | AATGTACAGTGATTTTTGTT+TGG | - | chr5.1:6423974-6423993 | None:intergenic | 25.0% |
| !! | ATTGGTTTATTAATGCTTCT+TGG | - | chr5.1:6424178-6424197 | None:intergenic | 25.0% |
| !! | CAAATGACAATCTTTTATGA+TGG | + | chr5.1:6423335-6423354 | MS.gene54190:CDS | 25.0% |
| !!! | GTTTTATTACAGTGTAATGA+AGG | + | chr5.1:6423280-6423299 | MS.gene54190:intron | 25.0% |
| AAAAAGAACATGTATGTACC+TGG | - | chr5.1:6423148-6423167 | None:intergenic | 30.0% | |
| AATCTTGGATTATCCAAAAG+TGG | - | chr5.1:6423817-6423836 | None:intergenic | 30.0% | |
| ATATTTGATTGAGAGAGATC+TGG | - | chr5.1:6423707-6423726 | None:intergenic | 30.0% | |
| ATTCAGATTTACCAATGACT+AGG | + | chr5.1:6423631-6423650 | MS.gene54190:intron | 30.0% | |
| CAAAATCAATTCAACTGATG+CGG | - | chr5.1:6423859-6423878 | None:intergenic | 30.0% | |
| CCATAAATAACAGGTATAGA+TGG | - | chr5.1:6423518-6423537 | None:intergenic | 30.0% | |
| GGAAGTTGTTGAAAATTCAT+AGG | - | chr5.1:6423087-6423106 | None:intergenic | 30.0% | |
| TCAAGTAGAATCAATTCTGT+AGG | - | chr5.1:6423882-6423901 | None:intergenic | 30.0% | |
| ! | AGTAAGCATAGTTGTTATTG+TGG | - | chr5.1:6423451-6423470 | None:intergenic | 30.0% |
| ! | CCATCTATACCTGTTATTTA+TGG | + | chr5.1:6423515-6423534 | MS.gene54190:CDS | 30.0% |
| ! | CTACAAGAGATTCCTATTTT+GGG | + | chr5.1:6423117-6423136 | MS.gene54190:CDS | 30.0% |
| ! | TGAAGTACTAGTAATTCCTT+TGG | - | chr5.1:6423423-6423442 | None:intergenic | 30.0% |
| AGTACTAGTAATTCCTTTGG+TGG | - | chr5.1:6423420-6423439 | None:intergenic | 35.0% | |
| CAATTGATGAATCCATGACA+TGG | + | chr5.1:6424194-6424213 | MS.gene54190:CDS | 35.0% | |
| CATGTCATGGATTCATCAAT+TGG | - | chr5.1:6424196-6424215 | None:intergenic | 35.0% | |
| CCAAAATAGGAATCTCTTGT+AGG | - | chr5.1:6423119-6423138 | None:intergenic | 35.0% | |
| CTACACTCAGATTTATGTTG+TGG | - | chr5.1:6424241-6424260 | None:intergenic | 35.0% | |
| CTTGGATTGCTTGTTTGATA+TGG | - | chr5.1:6424160-6424179 | None:intergenic | 35.0% | |
| GATTTATGTTGTGGTGATTG+TGG | - | chr5.1:6424232-6424251 | None:intergenic | 35.0% | |
| TGAAAAAGCCAAAGACATCA+TGG | + | chr5.1:6423388-6423407 | MS.gene54190:CDS | 35.0% | |
| ! | CCTACAAGAGATTCCTATTT+TGG | + | chr5.1:6423116-6423135 | MS.gene54190:CDS | 35.0% |
| !! | TGTAAGTTTGGTTCCACTTT+TGG | + | chr5.1:6423801-6423820 | MS.gene54190:intron | 35.0% |
| ACAGGTATAGATGGTACTTG+AGG | - | chr5.1:6423509-6423528 | None:intergenic | 40.0% | |
| AGTGAAGCTTTCCTAGTCAT+TGG | - | chr5.1:6423645-6423664 | None:intergenic | 40.0% | |
| ATCTCTTGTAGGAAGAGATG+AGG | - | chr5.1:6423108-6423127 | None:intergenic | 40.0% | |
| CAGGTATAGATGGTACTTGA+GGG | - | chr5.1:6423508-6423527 | None:intergenic | 40.0% | |
| CTAGGAAAGCTTCACTTCAT+CGG | + | chr5.1:6423649-6423668 | MS.gene54190:CDS | 40.0% | |
| GGAAAAGTCCATGATGTCTT+TGG | - | chr5.1:6423399-6423418 | None:intergenic | 40.0% | |
| TACCTGGAGTTTCCCAAAAT+AGG | - | chr5.1:6423132-6423151 | None:intergenic | 40.0% | |
| TCTTGGAGAAAAGGAAGGAT+AGG | + | chr5.1:6423673-6423692 | MS.gene54190:CDS | 40.0% | |
| TTAAAGTAACACATGGGTGG+AGG | - | chr5.1:6424269-6424288 | None:intergenic | 40.0% | |
| ! | ATCATGGACTTTTCCACCAA+AGG | + | chr5.1:6423404-6423423 | MS.gene54190:CDS | 40.0% |
| !! | TTCATCGGTTCTTGGAGAAA+AGG | + | chr5.1:6423664-6423683 | MS.gene54190:CDS | 40.0% |
| !! | TTCCTATTTTGGGAAACTCC+AGG | + | chr5.1:6423127-6423146 | MS.gene54190:CDS | 40.0% |
| !!! | ATGTCTTTGGCTTTTTCAGC+TGG | - | chr5.1:6423386-6423405 | None:intergenic | 40.0% |
| ATCCATGACATGGCTCTCTT+TGG | + | chr5.1:6424204-6424223 | MS.gene54190:CDS | 45.0% | |
| TCGGTTCTTGGAGAAAAGGA+AGG | + | chr5.1:6423668-6423687 | MS.gene54190:CDS | 45.0% | |
| TTGATTGAGAGAGATCTGGC+TGG | - | chr5.1:6423703-6423722 | None:intergenic | 45.0% | |
| !! | AGCTTCACTTCATCGGTTCT+TGG | + | chr5.1:6423656-6423675 | MS.gene54190:CDS | 45.0% |
| !! | AGGGTGCTTGATTTGTGTCA+TGG | + | chr5.1:6423565-6423584 | MS.gene54190:intron | 45.0% |
| CTTCTGCAGAATTGCAGCCA+GGG | + | chr5.1:6424132-6424151 | MS.gene54190:intron | 50.0% | |
| GCTTGTTTGATATGGTGCCC+TGG | - | chr5.1:6424152-6424171 | None:intergenic | 50.0% | |
| TGCCAAAGAGAGCCATGTCA+TGG | - | chr5.1:6424209-6424228 | None:intergenic | 50.0% | |
| GCTTCTGCAGAATTGCAGCC+AGG | + | chr5.1:6424131-6424150 | MS.gene54190:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 6423063 | 6424288 | 6423063 | ID=MS.gene54190 |
| chr5.1 | mRNA | 6423063 | 6424288 | 6423063 | ID=MS.gene54190.t1;Parent=MS.gene54190 |
| chr5.1 | exon | 6423063 | 6423148 | 6423063 | ID=MS.gene54190.t1.exon1;Parent=MS.gene54190.t1 |
| chr5.1 | CDS | 6423063 | 6423148 | 6423063 | ID=cds.MS.gene54190.t1;Parent=MS.gene54190.t1 |
| chr5.1 | exon | 6423292 | 6423536 | 6423292 | ID=MS.gene54190.t1.exon2;Parent=MS.gene54190.t1 |
| chr5.1 | CDS | 6423292 | 6423536 | 6423292 | ID=cds.MS.gene54190.t1;Parent=MS.gene54190.t1 |
| chr5.1 | exon | 6423637 | 6423694 | 6423637 | ID=MS.gene54190.t1.exon3;Parent=MS.gene54190.t1 |
| chr5.1 | CDS | 6423637 | 6423694 | 6423637 | ID=cds.MS.gene54190.t1;Parent=MS.gene54190.t1 |
| chr5.1 | exon | 6424141 | 6424288 | 6424141 | ID=MS.gene54190.t1.exon4;Parent=MS.gene54190.t1 |
| chr5.1 | CDS | 6424141 | 6424288 | 6424141 | ID=cds.MS.gene54190.t1;Parent=MS.gene54190.t1 |
| Gene Sequence |
| Protein sequence |
