AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54453.t1 MTR_7g007540 87.288 118 15 0 1 118 1 118 1.15e-71 209
MS.gene54453.t1 MTR_7g007630 89.831 118 12 0 1 118 666 783 6.41e-68 218
MS.gene54453.t1 MTR_7g407130 87.288 118 15 0 1 118 696 813 9.27e-66 212
MS.gene54453.t1 MTR_8g047210 93.220 118 8 0 1 118 768 885 8.06e-62 202
MS.gene54453.t1 MTR_8g047220 93.220 118 8 0 1 118 831 948 1.06e-61 202
MS.gene54453.t1 MTR_0491s0030 91.525 118 10 0 1 118 704 821 1.34e-60 198
MS.gene54453.t1 MTR_7g007560 81.356 118 22 0 1 118 653 770 2.66e-60 197
MS.gene54453.t1 MTR_8g047230 91.525 118 10 0 1 118 875 992 3.55e-60 198
MS.gene54453.t1 MTR_7g007620 86.325 117 16 0 1 117 620 736 1.94e-57 189
MS.gene54453.t1 MTR_7g009970 76.271 118 28 0 1 118 669 786 1.90e-55 184
MS.gene54453.t1 MTR_7g407090 83.051 118 20 0 1 118 651 768 3.46e-54 180
MS.gene54453.t1 MTR_7g010000 74.576 118 30 0 1 118 746 863 5.88e-54 180
MS.gene54453.t1 MTR_7g007580 81.651 109 20 0 10 118 3 111 6.84e-51 157
MS.gene54453.t1 MTR_7g013680 71.186 118 34 0 1 118 619 736 1.42e-49 167
MS.gene54453.t1 MTR_7g013350 66.102 118 40 0 1 118 295 412 1.76e-44 149
MS.gene54453.t1 MTR_8g047160 92.857 84 6 0 1 84 531 614 1.41e-39 139
MS.gene54453.t1 MTR_7g010010 61.017 118 27 1 1 118 616 714 4.62e-39 138
MS.gene54453.t1 MTR_7g009940 45.455 110 60 0 2 111 699 808 5.17e-27 104
MS.gene54453.t1 MTR_7g481630 50.000 114 51 3 1 109 80 192 1.42e-26 97.8
MS.gene54453.t1 MTR_1g039080 53.933 89 35 3 3 86 194 281 6.88e-26 98.2
MS.gene54453.t1 MTR_5g085700 49.107 112 52 2 3 110 922 1032 1.11e-25 100
MS.gene54453.t1 MTR_7g481590 50.000 114 51 3 1 109 203 315 7.40e-25 95.9
MS.gene54453.t1 MTR_7g081570 50.000 114 51 3 1 109 939 1051 7.72e-25 98.2
MS.gene54453.t1 MTR_4g037720 49.462 93 46 1 1 93 848 939 1.14e-24 97.8
MS.gene54453.t1 MTR_1g039310 50.549 91 39 2 3 88 1051 1140 3.08e-24 96.3
MS.gene54453.t1 MTR_4g037015 48.864 88 44 1 1 88 777 863 3.57e-24 96.3
MS.gene54453.t1 MTR_7g081720 48.696 115 52 3 1 109 964 1077 5.29e-24 95.9
MS.gene54453.t1 MTR_7g028432 48.864 88 44 1 1 88 853 939 5.29e-24 95.9
MS.gene54453.t1 MTR_7g045510 54.444 90 38 3 3 90 1092 1180 2.66e-23 93.6
MS.gene54453.t1 MTR_7g045710 52.273 88 40 2 3 90 973 1058 4.22e-23 93.2
MS.gene54453.t1 MTR_8g445960 44.086 93 46 2 1 88 63 154 4.92e-23 87.8
MS.gene54453.t1 MTR_8g044940 44.086 93 46 2 1 88 63 154 4.92e-23 87.8
MS.gene54453.t1 MTR_1g038890 51.613 93 39 3 1 88 1033 1124 4.95e-23 92.8
MS.gene54453.t1 MTR_7g039330 54.444 90 38 3 3 90 1190 1278 5.22e-23 92.8
MS.gene54453.t1 MTR_1g039220 50.505 99 40 2 1 91 1032 1129 5.66e-23 92.8
MS.gene54453.t1 MTR_1g039240 47.475 99 43 2 1 91 1058 1155 6.82e-23 92.4
MS.gene54453.t1 MTR_7g081480 46.903 113 55 2 1 109 949 1060 7.77e-23 92.4
MS.gene54453.t1 MTR_7g081410 45.614 114 56 3 1 109 946 1058 2.05e-22 91.3
MS.gene54453.t1 MTR_0400s0040 48.485 99 42 2 1 91 1058 1155 3.73e-22 90.5
MS.gene54453.t1 MTR_0365s0030 48.485 99 42 2 1 91 1058 1155 3.73e-22 90.5
MS.gene54453.t1 MTR_7g081410 45.614 114 56 3 1 109 826 938 5.02e-22 90.1
MS.gene54453.t1 MTR_7g446160 51.546 97 41 3 1 92 887 982 7.65e-22 89.7
MS.gene54453.t1 MTR_1g040615 47.475 99 43 2 1 91 2024 2121 1.46e-21 89.0
MS.gene54453.t1 MTR_1g040615 46.392 97 43 2 1 89 1025 1120 6.45e-21 87.0
MS.gene54453.t1 MTR_7g081780 44.737 114 58 2 1 110 947 1059 2.59e-21 88.2
MS.gene54453.t1 MTR_7g045860 47.778 90 44 2 3 90 985 1073 3.40e-21 87.8
MS.gene54453.t1 MTR_7g096980 41.593 113 61 3 1 109 1108 1219 3.96e-21 87.4
MS.gene54453.t1 MTR_1g040525 45.455 99 45 2 1 91 767 864 4.89e-21 87.4
MS.gene54453.t1 MTR_1g040545 46.465 99 44 2 1 91 907 1004 7.43e-21 86.7
MS.gene54453.t1 MTR_7g050990 46.939 98 43 3 1 90 819 915 8.94e-19 80.9
MS.gene54453.t1 MTR_0400s0030 42.708 96 49 2 1 91 791 885 2.47e-18 79.7
MS.gene54453.t1 MTR_0365s0020 42.708 96 49 2 1 91 791 885 2.47e-18 79.7
MS.gene54453.t1 MTR_7g051040 44.444 99 45 2 1 90 843 940 7.16e-18 78.2
MS.gene54453.t1 MTR_7g446190 42.683 82 41 2 1 77 815 895 9.85e-15 69.3
MS.gene54453.t1 MTR_1g040625 39.130 92 47 2 8 91 478 568 5.63e-14 67.0
MS.gene54453.t1 MTR_1g079520 37.895 95 49 3 1 86 946 1039 1.11e-12 63.5
MS.gene54453.t1 MTR_2g070020 33.884 121 64 4 1 108 987 1104 2.21e-12 62.8
MS.gene54453.t1 MTR_2g005810 35.577 104 54 4 1 96 865 963 5.09e-11 58.5
MS.gene54453.t1 MTR_1g047670 35.789 95 51 3 1 86 989 1082 9.34e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54453.t1 AT1G35710 44.444 90 49 1 1 90 1031 1119 2.48e-19 82.4
MS.gene54453.t1 AT4G08850 45.055 91 48 2 1 90 955 1044 3.47e-19 82.0
MS.gene54453.t1 AT2G33170 36.559 93 52 1 1 86 1003 1095 1.36e-12 63.2
MS.gene54453.t1 AT2G33170 36.559 93 52 1 1 86 1003 1095 1.36e-12 63.2
MS.gene54453.t1 AT3G49670 37.097 124 60 7 1 116 868 981 9.29e-12 60.8
MS.gene54453.t1 AT1G17230 35.484 93 52 2 1 86 977 1068 4.01e-11 58.9
MS.gene54453.t1 AT1G17230 35.000 100 56 3 1 93 977 1074 4.97e-11 58.9
MS.gene54453.t1 AT5G63930 37.634 93 50 2 1 86 983 1074 8.14e-11 58.2
MS.gene54453.t1 AT5G63930 37.634 93 50 2 1 86 919 1010 9.20e-11 58.2

Find 18 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTATAACTATGTCATTATT+TGG 0.194445 7.4:+92466631 None:intergenic
TGCAGTGAAACTTCACTAAA+AGG 0.310951 7.4:+92466490 None:intergenic
TGTGACACACGTTTCATTGT+TGG 0.385025 7.4:+92466547 None:intergenic
AAGTGTGATGTATATAGTTT+TGG 0.441933 7.4:-92466772 MS.gene54453:CDS
CTTTAGTTGGAAGGCACCCT+GGG 0.466628 7.4:-92466732 MS.gene54453:CDS
ACTTTAGTTGGAAGGCACCC+TGG 0.486816 7.4:-92466733 MS.gene54453:CDS
GTGGCACTTGAAACTTTAGT+TGG 0.497856 7.4:-92466745 MS.gene54453:CDS
ACTATGTCATTATTTGGAAG+TGG 0.499298 7.4:+92466637 None:intergenic
CACTTGAAACTTTAGTTGGA+AGG 0.502085 7.4:-92466741 MS.gene54453:CDS
TTCATTGTTGGTCGAGAACG+AGG 0.528233 7.4:+92466559 None:intergenic
AATGCTTAATCGTGGAAGCT+CGG 0.578382 7.4:+92466513 None:intergenic
AGTGATGACAATAAATCCCC+AGG 0.580045 7.4:+92466715 None:intergenic
CTAAAAGGAATGCTTAATCG+TGG 0.601346 7.4:+92466505 None:intergenic
TGACATAATTCATGTTGCCG+TGG 0.653027 7.4:-92466608 MS.gene54453:CDS
GTGATGACAATAAATCCCCA+GGG 0.663800 7.4:+92466716 None:intergenic
TAAGCATGCAAATGCTACCA+CGG 0.665989 7.4:+92466591 None:intergenic
TATTTGGAAGTGGAAGACGT+TGG 0.678619 7.4:+92466647 None:intergenic
TTTAGTTGGAAGGCACCCTG+GGG 0.725104 7.4:-92466731 MS.gene54453:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CGTATAACTATGTCATTATT+TGG + chr7.4:92466612-92466631 None:intergenic 25.0%
!! AAGTGTGATGTATATAGTTT+TGG - chr7.4:92466468-92466487 MS.gene54453:CDS 25.0%
ACTATGTCATTATTTGGAAG+TGG + chr7.4:92466606-92466625 None:intergenic 30.0%
!!! TGTATATAGTTTTGGTGTAG+TGG - chr7.4:92466476-92466495 MS.gene54453:CDS 30.0%
CACTTGAAACTTTAGTTGGA+AGG - chr7.4:92466499-92466518 MS.gene54453:CDS 35.0%
CTAAAAGGAATGCTTAATCG+TGG + chr7.4:92466738-92466757 None:intergenic 35.0%
TGCAGTGAAACTTCACTAAA+AGG + chr7.4:92466753-92466772 None:intergenic 35.0%
AATGCTTAATCGTGGAAGCT+CGG + chr7.4:92466730-92466749 None:intergenic 40.0%
AGTGATGACAATAAATCCCC+AGG + chr7.4:92466528-92466547 None:intergenic 40.0%
GTGATGACAATAAATCCCCA+GGG + chr7.4:92466527-92466546 None:intergenic 40.0%
GTGGCACTTGAAACTTTAGT+TGG - chr7.4:92466495-92466514 MS.gene54453:CDS 40.0%
TAAGCATGCAAATGCTACCA+CGG + chr7.4:92466652-92466671 None:intergenic 40.0%
TATTTGGAAGTGGAAGACGT+TGG + chr7.4:92466596-92466615 None:intergenic 40.0%
TGACATAATTCATGTTGCCG+TGG - chr7.4:92466632-92466651 MS.gene54453:CDS 40.0%
TGTGACACACGTTTCATTGT+TGG + chr7.4:92466696-92466715 None:intergenic 40.0%
TTCATTGTTGGTCGAGAACG+AGG + chr7.4:92466684-92466703 None:intergenic 45.0%
!! ACTTTAGTTGGAAGGCACCC+TGG - chr7.4:92466507-92466526 MS.gene54453:CDS 50.0%
!! CTTTAGTTGGAAGGCACCCT+GGG - chr7.4:92466508-92466527 MS.gene54453:CDS 50.0%
!! TTTAGTTGGAAGGCACCCTG+GGG - chr7.4:92466509-92466528 MS.gene54453:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 92466453 92466809 92466453 ID=MS.gene54453
chr7.4 mRNA 92466453 92466809 92466453 ID=MS.gene54453.t1;Parent=MS.gene54453
chr7.4 exon 92466453 92466809 92466453 ID=MS.gene54453.t1.exon1;Parent=MS.gene54453.t1
chr7.4 CDS 92466453 92466809 92466453 ID=cds.MS.gene54453.t1;Parent=MS.gene54453.t1
Gene Sequence

>MS.gene54453

ATGGCGGTGAATGAAAAGTGTGATGTATATAGTTTTGGTGTAGTGGCACTTGAAACTTTAGTTGGAAGGCACCCTGGGGATTTATTGTCATCACTTCAATCAACATCGACACAAAGTGTCAAACTATGTCAAGTATTAGACCAACGTCTTCCACTTCCAAATAATGACATAGTTATACGTGACATAATTCATGTTGCCGTGGTAGCATTTGCATGCTTAAATGTCAATCCTCGTTCTCGACCAACAATGAAACGTGTGTCACAAAGTTTTGTCACCGAGCTTCCACGATTAAGCATTCCTTTTAGTGAAGTTTCACTGCAGCAACTCATGAGTCAAGAACTCAAAGAATTATTTTAA
Protein sequence

>MS.gene54453.t1

MAVNEKCDVYSFGVVALETLVGRHPGDLLSSLQSTSTQSVKLCQVLDQRLPLPNNDIVIRDIIHVAVVAFACLNVNPRSRPTMKRVSQSFVTELPRLSIPFSEVSLQQLMSQELKELF