AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54601


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54601.t1 MTR_4g051538 54.980 251 92 5 1 248 1 233 2.63e-81 244
MS.gene54601.t1 MTR_3g104630 56.757 148 57 5 94 238 3 146 4.55e-42 140
MS.gene54601.t1 MTR_3g052920 36.527 167 89 5 1 150 1 167 7.16e-22 90.9
MS.gene54601.t1 MTR_2g030740 37.126 167 88 4 1 150 1 167 1.04e-21 90.5
MS.gene54601.t1 MTR_1g105910 36.646 161 85 4 1 144 1 161 1.14e-21 90.5
MS.gene54601.t1 MTR_1g012570 37.356 174 87 8 1 156 1 170 1.25e-21 90.5
MS.gene54601.t1 MTR_8g043650 36.424 151 81 3 1 136 1 151 3.11e-21 89.4
MS.gene54601.t1 MTR_1g077360 30.457 197 113 5 1 187 1 183 3.98e-21 89.0
MS.gene54601.t1 MTR_5g053390 32.075 212 116 8 1 201 1 195 6.40e-21 88.6
MS.gene54601.t1 MTR_1g106070 36.646 161 85 5 1 144 1 161 1.34e-20 86.3
MS.gene54601.t1 MTR_1g108510 32.530 166 99 3 1 156 1 163 1.89e-20 87.0
MS.gene54601.t1 MTR_3g030770 32.530 166 99 3 1 156 1 163 3.13e-20 86.3
MS.gene54601.t1 MTR_3g080940 33.133 166 96 4 1 155 1 162 1.72e-19 84.0
MS.gene54601.t1 MTR_1g105920 35.762 151 82 3 1 136 1 151 2.93e-19 84.0
MS.gene54601.t1 MTR_2g085280 30.625 160 100 3 1 151 1 158 4.89e-19 83.6
MS.gene54601.t1 MTR_2g085250 30.625 160 100 3 1 151 1 158 5.31e-19 83.2
MS.gene54601.t1 MTR_8g086290 31.579 171 84 4 4 156 7 162 1.23e-18 80.9
MS.gene54601.t1 MTR_1g075600 30.682 176 106 4 1 163 1 173 1.52e-18 82.0
MS.gene54601.t1 MTR_3g052870 51.389 72 35 0 1 72 1 72 2.62e-18 80.9
MS.gene54601.t1 MTR_5g045560 33.577 137 81 3 1 127 1 137 2.65e-18 81.3
MS.gene54601.t1 MTR_1g108580 34.132 167 81 6 1 144 1 161 2.98e-18 81.3
MS.gene54601.t1 MTR_2g105290 30.818 159 101 3 4 156 7 162 1.66e-17 77.8
MS.gene54601.t1 MTR_5g055100 29.536 237 127 10 1 202 1 232 2.06e-17 79.0
MS.gene54601.t1 MTR_2g093190 31.677 161 93 4 1 144 1 161 1.05e-16 77.0
MS.gene54601.t1 MTR_0121s0100 42.857 77 44 0 1 77 1 77 7.05e-16 72.4
MS.gene54601.t1 MTR_3g030780 39.252 107 62 2 1 104 1 107 2.63e-15 70.9
MS.gene54601.t1 MTR_4g094638 49.275 69 35 0 9 77 2 70 2.82e-15 72.0
MS.gene54601.t1 MTR_4g094632 34.028 144 83 4 9 151 2 134 2.74e-14 68.9
MS.gene54601.t1 MTR_1g047550 36.885 122 61 3 9 118 2 119 2.98e-14 69.3
MS.gene54601.t1 MTR_1g105905 29.189 185 112 6 18 185 1 183 7.43e-14 68.9
MS.gene54601.t1 MTR_8g046350 41.667 72 42 0 9 80 2 73 8.01e-14 67.8
MS.gene54601.t1 MTR_8g051580 32.143 140 71 4 9 126 2 139 9.28e-14 67.8
MS.gene54601.t1 MTR_8g079502 41.250 80 46 1 9 87 2 81 1.57e-13 67.4
MS.gene54601.t1 MTR_0121s0080 30.120 166 99 4 1 149 1 166 1.75e-13 68.2
MS.gene54601.t1 MTR_3g067875 49.091 55 28 0 18 72 1 55 3.70e-13 63.2
MS.gene54601.t1 MTR_8g022970 32.515 163 94 4 8 159 17 174 4.50e-13 66.2
MS.gene54601.t1 MTR_1g063160 36.134 119 66 3 9 118 2 119 1.76e-12 64.3
MS.gene54601.t1 MTR_1g054265 40.000 70 42 0 9 78 2 71 6.28e-12 62.8
MS.gene54601.t1 MTR_4g131030 38.889 72 44 0 9 80 2 73 9.67e-12 63.2
MS.gene54601.t1 MTR_1g029670 48.438 64 33 0 9 72 2 65 2.72e-11 61.2
MS.gene54601.t1 MTR_7g028448 29.921 127 83 4 26 148 29 153 5.82e-11 60.1
MS.gene54601.t1 MTR_4g036915 29.921 127 83 4 26 148 29 153 5.82e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54601.t1 AT4G36590 34.132 167 93 3 5 156 3 167 2.36e-21 90.1
MS.gene54601.t1 AT1G29962 35.227 176 96 4 1 161 1 173 2.56e-21 88.2
MS.gene54601.t1 AT2G24840 32.474 194 109 6 7 187 61 245 1.49e-20 88.2
MS.gene54601.t1 AT1G28460 35.260 173 97 4 1 161 1 170 1.98e-19 83.2
MS.gene54601.t1 AT5G60440 32.547 212 115 7 5 205 3 197 8.52e-18 81.3
MS.gene54601.t1 AT1G01530 37.288 118 68 2 5 117 3 119 1.11e-17 80.1
MS.gene54601.t1 AT2G34440 32.203 177 97 7 9 174 2 166 5.75e-17 76.3
MS.gene54601.t1 AT1G28450 32.759 174 99 4 1 159 1 171 1.79e-16 75.5
MS.gene54601.t1 AT3G04100 41.573 89 49 1 2 90 7 92 1.97e-15 73.2
MS.gene54601.t1 AT1G65360 33.019 106 67 1 5 106 3 108 8.89e-15 71.6
MS.gene54601.t1 AT3G66656 47.692 65 34 0 9 73 2 66 1.83e-13 67.0
MS.gene54601.t1 AT1G47760 39.759 83 47 1 9 91 2 81 3.79e-13 66.2
MS.gene54601.t1 AT4G11880 49.254 67 32 1 10 76 3 67 8.30e-11 59.7
MS.gene54601.t1 AT4G11880 49.254 67 32 1 10 76 3 67 8.30e-11 59.7

Find 46 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCATATCATTCATCTTT+AGG 0.191781 4.3:+37967912 None:intergenic
ACAAATCCTCCTTATGTCTT+TGG 0.244037 4.3:-37968329 MS.gene54601:CDS
AACAACAACGGTTATGAAAT+TGG 0.254239 4.3:-37968302 MS.gene54601:CDS
TCAGGAAAGTTTGAAGGCTT+TGG 0.277217 4.3:-37968656 MS.gene54601:CDS
CAAATCCTCCTTATGTCTTT+GGG 0.283816 4.3:-37968328 MS.gene54601:CDS
GAAGGTTCTCCATAACAATA+TGG 0.353627 4.3:+37968814 None:intergenic
CTTTGGAAAGTTATGGCTAT+TGG 0.358280 4.3:-37968639 MS.gene54601:CDS
AAAATTGGACACATCTTTAG+TGG 0.370003 4.3:-37968569 MS.gene54601:CDS
GATGTTATTGCTTCTCTTGA+AGG 0.392101 4.3:-37968008 MS.gene54601:CDS
TCTGAATTATCAACACTTTG+TGG 0.392582 4.3:-37968877 MS.gene54601:CDS
TTGAAGGCTTTGGAAAGTTA+TGG 0.393387 4.3:-37968646 MS.gene54601:CDS
GCATGTTGCGGAAGCTCATC+AGG 0.403902 4.3:-37968674 MS.gene54601:CDS
CTTGCTATGGATTGAGAAGA+AGG 0.408194 4.3:+37968796 None:intergenic
GCAGGAAAACCATATTGTTA+TGG 0.420932 4.3:-37968823 MS.gene54601:CDS
TCACTTGCACGGTCAAGGTT+TGG 0.423194 4.3:+37967966 None:intergenic
CTGAATTATCAACACTTTGT+GGG 0.424852 4.3:-37968876 MS.gene54601:CDS
CTACCTCTTGATGTCCTCTT+CGG 0.429458 4.3:+37968988 None:intergenic
CTTCAAAATTGTTCTTGCTA+TGG 0.431100 4.3:+37968783 None:intergenic
CATTTCCACTTTCACTTGCA+CGG 0.448306 4.3:+37967955 None:intergenic
ATTCTTATTATTTCTCCAGC+AGG 0.453510 4.3:-37968841 MS.gene54601:CDS
CACTTTCACTTGCACGGTCA+AGG 0.460125 4.3:+37967961 None:intergenic
AATTTCATAACCGTTGTTGT+TGG 0.462889 4.3:+37968304 None:intergenic
AGACATAAGGAGGATTTGTC+TGG 0.473975 4.3:+37968333 None:intergenic
CTTGACCGTGCAAGTGAAAG+TGG 0.475104 4.3:-37967960 MS.gene54601:CDS
TTGTTGGCCCCAAAGACATA+AGG 0.478695 4.3:+37968320 None:intergenic
TTTAATTCTTCTTCTACCGA+AGG 0.479866 4.3:+37968751 None:intergenic
AGCTCATCAGGAAAGTTTGA+AGG 0.487303 4.3:-37968662 MS.gene54601:CDS
CGTGCAAGTGAAAGTGGAAA+TGG 0.489776 4.3:-37967954 MS.gene54601:CDS
ATTTATGATCCAAAAGGAAA+AGG 0.506756 4.3:-37968035 MS.gene54601:CDS
TCCCAGATTTATGATCCAAA+AGG 0.515467 4.3:-37968041 MS.gene54601:CDS
TTTGAAGAATGAAAACCCTT+CGG 0.527588 4.3:-37968767 MS.gene54601:CDS
TCTTGCATAGATGAACTGAA+TGG 0.533604 4.3:-37968359 MS.gene54601:CDS
TTAATTCTTCTTCTACCGAA+GGG 0.546382 4.3:+37968752 None:intergenic
ATAAGTAGTGTCTTAAGCAA+TGG 0.548667 4.3:-37968529 MS.gene54601:CDS
TGAATTATCAACACTTTGTG+GGG 0.569118 4.3:-37968875 MS.gene54601:CDS
GTCTTTGGGGCCAACAACAA+CGG 0.574083 4.3:-37968314 MS.gene54601:CDS
GGAGGAGAGATTAATCCTCA+TGG 0.604055 4.3:-37968508 MS.gene54601:intron
AAATCCTCCTTATGTCTTTG+GGG 0.607760 4.3:-37968327 MS.gene54601:CDS
AGTAGTGTCTTAAGCAATGG+AGG 0.611596 4.3:-37968526 MS.gene54601:CDS
TTGGCCCCAAAGACATAAGG+AGG 0.611952 4.3:+37968323 None:intergenic
ACAAAGTGTTGATAATTCAG+AGG 0.616841 4.3:+37968879 None:intergenic
TGATCCAAAAGGAAAAGGCA+AGG 0.624272 4.3:-37968030 MS.gene54601:CDS
TTTGGATGAAAAGCATGTTG+CGG 0.629913 4.3:-37968686 MS.gene54601:CDS
TGGTTAAGATGGCTCCGAAG+AGG 0.660173 4.3:-37969002 None:intergenic
GCTCCGAAGAGGACATCAAG+AGG 0.673948 4.3:-37968991 MS.gene54601:CDS
GAAGGTAGAAATAAAACACA+AGG 0.705496 4.3:-37967990 MS.gene54601:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATTTATGAAAAAG+TGG - chr4.3:37968509-37968528 MS.gene54601:CDS 15.0%
!! GAAATTATACAATATGTGTT+AGG + chr4.3:37968833-37968852 None:intergenic 20.0%
!! TTATGACCAGTTAAAAAAAT+TGG - chr4.3:37968330-37968349 MS.gene54601:CDS 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG + chr4.3:37968785-37968804 None:intergenic 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG + chr4.3:37968784-37968803 None:intergenic 20.0%
!!! TACTCTTGTTTATATGAATT+TGG + chr4.3:37968658-37968677 None:intergenic 20.0%
! AGTATAGATATTTGATGTGT+TGG + chr4.3:37968699-37968718 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG - chr4.3:37968879-37968898 MS.gene54601:CDS 25.0%
! GCATTAAAAATGGTTAAAGT+TGG + chr4.3:37968723-37968742 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG + chr4.3:37968733-37968752 None:intergenic 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG + chr4.3:37968339-37968358 None:intergenic 25.0%
AAAATTGGACACATCTTTAG+TGG - chr4.3:37968345-37968364 MS.gene54601:CDS 30.0%
AACAACAACGGTTATGAAAT+TGG - chr4.3:37968612-37968631 MS.gene54601:CDS 30.0%
AATTTCATAACCGTTGTTGT+TGG + chr4.3:37968613-37968632 None:intergenic 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG - chr4.3:37968385-37968404 MS.gene54601:intron 30.0%
CTGAATTATCAACACTTTGT+GGG - chr4.3:37968038-37968057 MS.gene54601:CDS 30.0%
CTTCAAAATTGTTCTTGCTA+TGG + chr4.3:37968134-37968153 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG - chr4.3:37968924-37968943 MS.gene54601:CDS 30.0%
TCTGAATTATCAACACTTTG+TGG - chr4.3:37968037-37968056 MS.gene54601:CDS 30.0%
TGAATTATCAACACTTTGTG+GGG - chr4.3:37968039-37968058 MS.gene54601:CDS 30.0%
TGTACATCTTCTAATCAACT+TGG - chr4.3:37968433-37968452 MS.gene54601:intron 30.0%
TTAATTCTTCTTCTACCGAA+GGG + chr4.3:37968165-37968184 None:intergenic 30.0%
TTTAATTCTTCTTCTACCGA+AGG + chr4.3:37968166-37968185 None:intergenic 30.0%
TTTGAAGAATGAAAACCCTT+CGG - chr4.3:37968147-37968166 MS.gene54601:intron 30.0%
! ATTCTTATTATTTCTCCAGC+AGG - chr4.3:37968073-37968092 MS.gene54601:intron 30.0%
! CACAAAAAATGATGAGCTTT+TGG - chr4.3:37968210-37968229 MS.gene54601:intron 30.0%
! GCATTTTCAAAAAGAAAGTC+TGG - chr4.3:37967998-37968017 MS.gene54601:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG + chr4.3:37968877-37968896 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG + chr4.3:37968878-37968897 None:intergenic 30.0%
!! ACAAAGTGTTGATAATTCAG+AGG + chr4.3:37968038-37968057 None:intergenic 30.0%
AAATCCTCCTTATGTCTTTG+GGG - chr4.3:37968587-37968606 MS.gene54601:CDS 35.0%
ACAAATCCTCCTTATGTCTT+TGG - chr4.3:37968585-37968604 MS.gene54601:CDS 35.0%
CAAATCCTCCTTATGTCTTT+GGG - chr4.3:37968586-37968605 MS.gene54601:CDS 35.0%
GAAGGTTCTCCATAACAATA+TGG + chr4.3:37968103-37968122 None:intergenic 35.0%
GATGTTATTGCTTCTCTTGA+AGG - chr4.3:37968906-37968925 MS.gene54601:CDS 35.0%
GCAGGAAAACCATATTGTTA+TGG - chr4.3:37968091-37968110 MS.gene54601:intron 35.0%
TATGTGTTAGGTTTATCGCA+TGG + chr4.3:37968821-37968840 None:intergenic 35.0%
TCCCAGATTTATGATCCAAA+AGG - chr4.3:37968873-37968892 MS.gene54601:CDS 35.0%
TCTTGCATAGATGAACTGAA+TGG - chr4.3:37968555-37968574 MS.gene54601:CDS 35.0%
TTTCTTGTCATTGAAGCTCA+TGG - chr4.3:37968849-37968868 MS.gene54601:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG - chr4.3:37968275-37968294 MS.gene54601:intron 35.0%
! TAACAATATGGTTTTCCTGC+TGG + chr4.3:37968091-37968110 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG - chr4.3:37968228-37968247 MS.gene54601:intron 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG - chr4.3:37968268-37968287 MS.gene54601:intron 35.0%
AGACATAAGGAGGATTTGTC+TGG + chr4.3:37968584-37968603 None:intergenic 40.0%
AGCTCATCAGGAAAGTTTGA+AGG - chr4.3:37968252-37968271 MS.gene54601:intron 40.0%
AGTAGTGTCTTAAGCAATGG+AGG - chr4.3:37968388-37968407 MS.gene54601:intron 40.0%
CATTTCCACTTTCACTTGCA+CGG + chr4.3:37968962-37968981 None:intergenic 40.0%
TCAGGAAAGTTTGAAGGCTT+TGG - chr4.3:37968258-37968277 MS.gene54601:intron 40.0%
TGATCCAAAAGGAAAAGGCA+AGG - chr4.3:37968884-37968903 MS.gene54601:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG + chr4.3:37968891-37968910 None:intergenic 40.0%
! CTTGCTATGGATTGAGAAGA+AGG + chr4.3:37968121-37968140 None:intergenic 40.0%
CGTGCAAGTGAAAGTGGAAA+TGG - chr4.3:37968960-37968979 MS.gene54601:CDS 45.0%
CTACCTCTTGATGTCCTCTT+CGG + chr4.3:37967929-37967948 None:intergenic 45.0%
GAAGATGTACACGTACCATG+AGG + chr4.3:37968424-37968443 None:intergenic 45.0%
GGAGGAGAGATTAATCCTCA+TGG - chr4.3:37968406-37968425 MS.gene54601:intron 45.0%
TTGTTGGCCCCAAAGACATA+AGG + chr4.3:37968597-37968616 None:intergenic 45.0%
CACTTTCACTTGCACGGTCA+AGG + chr4.3:37968956-37968975 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG - chr4.3:37968954-37968973 MS.gene54601:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG + chr4.3:37968951-37968970 None:intergenic 50.0%
TTGGCCCCAAAGACATAAGG+AGG + chr4.3:37968594-37968613 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG - chr4.3:37968600-37968619 MS.gene54601:CDS 50.0%
GCATGTTGCGGAAGCTCATC+AGG - chr4.3:37968240-37968259 MS.gene54601:intron 55.0%
GCTCCGAAGAGGACATCAAG+AGG - chr4.3:37967923-37967942 MS.gene54601:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 37967920 37969016 37967920 ID=MS.gene54601
chr4.3 mRNA 37967920 37969016 37967920 ID=MS.gene54601.t1;Parent=MS.gene54601
chr4.3 exon 37968509 37969016 37968509 ID=MS.gene54601.t1.exon1;Parent=MS.gene54601.t1
chr4.3 CDS 37968509 37969016 37968509 ID=cds.MS.gene54601.t1;Parent=MS.gene54601.t1
chr4.3 exon 37968297 37968386 37968297 ID=MS.gene54601.t1.exon2;Parent=MS.gene54601.t1
chr4.3 CDS 37968297 37968386 37968297 ID=cds.MS.gene54601.t1;Parent=MS.gene54601.t1
chr4.3 exon 37967920 37968071 37967920 ID=MS.gene54601.t1.exon3;Parent=MS.gene54601.t1
chr4.3 CDS 37967920 37968071 37967920 ID=cds.MS.gene54601.t1;Parent=MS.gene54601.t1
Gene Sequence

>MS.gene54601

ATGGCTCCGAAGAGGACATCAAGAGGTAGACAAAAAAGAGAAATGAAGAAGATTGAGAGAGAATGCGATCTTTTAGTAGCATTTTCAAAAAGAAAGTCTGGCATTTACAACAAAGCCTCTGAATTATCAACACTTTGTGGGGCTTTAGTTGATATTCTTATTATTTCTCCAGCAGGAAAACCATATTGTTATGGAGAACCTTCTTCTCAATCCATAGCAAGAACAATTTTGAAGAATGAAAACCCTTCGGTAGAAGAAGAATTAAAGCAACTAAAGATTAACAAAGTTAACACAAAAAATGATGAGCTTTTGGATGAAAAGCATGTTGCGGAAGCTCATCAGGAAAGTTTGAAGGCTTTGGAAAGTTATGGCTATTGGTCGATCAATGAGAAAGATGTTGAAAGCTTTGATTATGACCAGTTAAAAAAATTGGACACATCTTTAGTGGACATTGCAAATAAATTGATAAGTAGTGTCTTAAGCAATGGAGGAGAGATTAATCCTCATGGTACGTGTACATCTTCTAATCAACTTGGTCTTATATTTGATATATTAATGATGTCAAATGATTTCATTTCATGTTTTCTGTCTAATTTATTTATGAAAAAGTGGTTATAATTTTATTTGCAGCAATTTCTTGCATAGATGAACTGAATGGAGTCCAGACAAATCCTCCTTATGTCTTTGGGGCCAACAACAACGGTTATGAAATTGGTGCAAGTAAGAAATCTCATTCCAAATTCATATAAACAAGAGTAATACATATTACTCGCACTCCAACACATCAAATATCTATACTGCCAACTTTAACCATTTTTAATGCACTTATGAGATAAATCTTGTTTATTTTTTCATTTTGATCCCAAAAAAAGATATGAAGACATTAAATTTTAATATTCCATGCGATAAACCTAACACATATTGTATAATTTCTTGTCATTGAAGCTCATGGATCCCAGATTTATGATCCAAAAGGAAAAGGCAAGGATGTTATTGCTTCTCTTGAAGGTAGAAATAAAACACAAGGACCAAACCTTGACCGTGCAAGTGAAAGTGGAAATGGAAGTGATGTTGATCATTCTCCTAAAGATGAATGA

Protein sequence

>MS.gene54601.t1

MAPKRTSRGRQKREMKKIERECDLLVAFSKRKSGIYNKASELSTLCGALVDILIISPAGKPYCYGEPSSQSIARTILKNENPSVEEELKQLKINKVNTKNDELLDEKHVAEAHQESLKALESYGYWSINEKDVESFDYDQLKKLDTSLVDIANKLISSVLSNGGEINPHAISCIDELNGVQTNPPYVFGANNNGYEIGATHGSQIYDPKGKGKDVIASLEGRNKTQGPNLDRASESGNGSDVDHSPKDE