AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54754

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54754.t1 MTR_1g095850 98.261 230 3 1 1 230 1 229 3.40e-165 455
MS.gene54754.t1 MTR_2g093310 62.911 213 56 6 26 230 1 198 8.41e-83 245
MS.gene54754.t1 MTR_4g060950 67.416 178 45 7 64 230 15 190 6.71e-77 230
MS.gene54754.t1 MTR_2g068760 70.909 110 32 0 71 180 11 120 8.06e-54 170
MS.gene54754.t1 MTR_4g083680 67.890 109 35 0 72 180 14 122 3.23e-51 164
MS.gene54754.t1 MTR_3g071590 71.845 103 29 0 72 174 7 109 1.37e-50 162
MS.gene54754.t1 MTR_5g083230 65.487 113 39 0 72 184 5 117 2.31e-50 162
MS.gene54754.t1 MTR_3g031660 66.055 109 37 0 72 180 6 114 1.31e-49 160
MS.gene54754.t1 MTR_5g083960 54.386 114 52 0 71 184 37 150 2.77e-41 139
MS.gene54754.t1 MTR_3g077240 59.048 105 43 0 72 176 9 113 4.87e-41 139
MS.gene54754.t1 MTR_3g073690 58.824 102 42 0 72 173 8 109 9.25e-41 138
MS.gene54754.t1 MTR_4g105170 59.223 103 42 0 72 174 10 112 2.34e-40 137
MS.gene54754.t1 MTR_6g005070 52.174 115 55 0 72 186 8 122 3.03e-40 135
MS.gene54754.t1 MTR_7g033800 58.252 103 43 0 72 174 10 112 1.12e-39 135
MS.gene54754.t1 MTR_3g094690 52.101 119 56 1 64 181 37 155 1.23e-39 137
MS.gene54754.t1 MTR_5g017950 57.843 102 43 0 72 173 7 108 1.25e-38 136
MS.gene54754.t1 MTR_5g075020 59.459 111 45 0 71 181 41 151 1.91e-38 133
MS.gene54754.t1 MTR_6g005080 54.286 105 48 0 72 176 9 113 3.21e-38 130
MS.gene54754.t1 MTR_5g075020 59.459 111 45 0 71 181 45 155 3.88e-38 132
MS.gene54754.t1 MTR_3g094690 51.261 119 56 2 64 181 37 154 4.76e-37 130
MS.gene54754.t1 MTR_3g452660 53.774 106 49 0 72 177 6 111 1.12e-36 130
MS.gene54754.t1 MTR_5g083010 55.882 102 45 0 72 173 10 111 4.57e-36 125
MS.gene54754.t1 MTR_3g071420 56.566 99 43 0 72 170 13 111 2.32e-35 124
MS.gene54754.t1 MTR_7g028905 45.690 116 61 1 69 182 1 116 3.99e-33 116
MS.gene54754.t1 MTR_8g040900 57.843 102 43 0 72 173 6 107 3.66e-32 117
MS.gene54754.t1 MTR_7g074990 50.000 102 50 1 73 173 20 121 1.84e-31 115
MS.gene54754.t1 MTR_7g096530 51.456 103 49 1 72 173 13 115 2.77e-31 114
MS.gene54754.t1 MTR_8g036105 50.943 106 51 1 72 176 17 122 3.71e-31 114
MS.gene54754.t1 MTR_6g027710 43.697 119 64 2 70 186 37 154 4.42e-31 114
MS.gene54754.t1 MTR_8g079660 48.077 104 54 0 70 173 5 108 2.25e-30 113
MS.gene54754.t1 MTR_1g070220 44.531 128 58 2 48 174 2 117 3.37e-30 111
MS.gene54754.t1 MTR_8g036085 48.544 103 52 1 72 173 22 124 7.03e-30 111
MS.gene54754.t1 MTR_1g070205 51.961 102 46 2 73 171 12 113 1.86e-29 110
MS.gene54754.t1 MTR_8g079620 48.077 104 54 0 70 173 5 108 4.14e-29 109
MS.gene54754.t1 MTR_5g093010 41.905 105 61 0 69 173 1 105 5.38e-29 106
MS.gene54754.t1 MTR_7g075230 50.476 105 49 2 72 173 11 115 1.54e-28 107
MS.gene54754.t1 MTR_7g096610 51.546 97 46 1 72 167 7 103 2.29e-28 107
MS.gene54754.t1 MTR_6g027700 37.671 146 79 4 70 212 4 140 5.06e-27 103
MS.gene54754.t1 MTR_8g079580 46.226 106 56 1 70 174 5 110 6.70e-27 103
MS.gene54754.t1 MTR_5g080470 46.875 96 50 1 72 166 6 101 4.71e-26 100
MS.gene54754.t1 MTR_6g011250 42.609 115 61 2 74 183 10 124 2.33e-25 98.2
MS.gene54754.t1 MTR_6g011200 42.623 122 59 3 74 187 10 128 1.65e-24 97.8
MS.gene54754.t1 MTR_4g107450 43.000 100 57 0 74 173 11 110 1.35e-23 96.3
MS.gene54754.t1 MTR_6g011230 40.187 107 62 1 74 180 12 116 1.02e-21 89.4
MS.gene54754.t1 MTR_4g099230 40.000 115 58 3 74 183 3 111 1.25e-20 85.1
MS.gene54754.t1 MTR_8g017090 39.000 100 61 0 74 173 13 112 1.43e-19 85.5
MS.gene54754.t1 MTR_2g100020 40.860 93 55 0 74 166 20 112 6.12e-19 83.6
MS.gene54754.t1 MTR_5g085390 38.614 101 61 1 74 173 10 110 4.95e-16 74.7
MS.gene54754.t1 MTR_4g088035 32.258 93 63 0 69 161 2 94 1.37e-14 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54754.t1 AT1G07900 68.072 166 44 5 69 230 29 189 2.77e-74 223
MS.gene54754.t1 AT2G28500 63.187 182 47 4 69 230 51 232 3.28e-71 217
MS.gene54754.t1 AT2G30130 68.519 108 34 0 72 179 7 114 3.02e-50 162
MS.gene54754.t1 AT1G16530 65.421 107 36 1 71 176 12 118 2.68e-47 154
MS.gene54754.t1 AT1G31320 67.273 110 36 0 72 181 12 121 1.96e-43 144
MS.gene54754.t1 AT5G63090 60.194 103 41 0 72 174 10 112 3.61e-41 139
MS.gene54754.t1 AT5G63090 60.194 103 41 0 72 174 10 112 3.61e-41 139
MS.gene54754.t1 AT5G63090 60.194 103 41 0 72 174 10 112 3.61e-41 139
MS.gene54754.t1 AT5G63090 60.194 103 41 0 72 174 10 112 3.61e-41 139
MS.gene54754.t1 AT2G23660 58.654 104 43 0 72 175 4 107 6.21e-41 142
MS.gene54754.t1 AT2G23660 58.654 104 43 0 72 175 4 107 6.21e-41 142
MS.gene54754.t1 AT2G23660 58.654 104 43 0 72 175 4 107 6.21e-41 142
MS.gene54754.t1 AT2G30340 52.632 114 54 0 71 184 51 164 9.19e-41 140
MS.gene54754.t1 AT2G30340 52.632 114 54 0 71 184 50 163 9.66e-41 140
MS.gene54754.t1 AT3G27650 58.654 104 43 0 72 175 38 141 2.84e-40 136
MS.gene54754.t1 AT5G66870 55.357 112 50 0 72 183 6 117 1.16e-38 136
MS.gene54754.t1 AT2G40470 49.296 142 68 2 61 200 6 145 4.24e-38 131
MS.gene54754.t1 AT2G40470 49.296 142 68 2 61 200 31 170 9.23e-38 131
MS.gene54754.t1 AT3G26620 56.000 100 44 0 74 173 6 105 1.53e-37 127
MS.gene54754.t1 AT3G26660 56.000 100 44 0 74 173 6 105 1.54e-37 127
MS.gene54754.t1 AT3G11090 54.369 103 47 0 72 174 10 112 8.45e-37 127
MS.gene54754.t1 AT1G65620 59.804 102 41 0 72 173 8 109 1.89e-33 119
MS.gene54754.t1 AT1G65620 59.804 102 41 0 72 173 8 109 1.89e-33 119
MS.gene54754.t1 AT1G65620 59.804 102 41 0 72 173 8 109 1.89e-33 119
MS.gene54754.t1 AT1G65620 59.804 102 41 0 72 173 8 109 1.89e-33 119
MS.gene54754.t1 AT1G65620 59.804 102 41 0 72 173 8 109 1.89e-33 119
MS.gene54754.t1 AT4G00210 54.369 103 46 1 72 173 10 112 2.87e-33 120
MS.gene54754.t1 AT3G03760 48.214 112 57 1 72 182 50 161 4.54e-33 120
MS.gene54754.t1 AT4G00220 50.980 102 49 1 76 176 20 121 1.28e-30 113
MS.gene54754.t1 AT2G31310 48.077 104 53 1 70 172 4 107 2.53e-29 108
MS.gene54754.t1 AT2G45410 54.545 99 44 1 76 173 19 117 3.24e-29 108
MS.gene54754.t1 AT2G42430 46.667 105 55 1 72 175 14 118 3.94e-29 109
MS.gene54754.t1 AT2G42440 54.167 96 43 1 72 166 6 101 5.41e-29 109
MS.gene54754.t1 AT2G45420 49.020 102 51 1 76 176 40 141 3.54e-28 107
MS.gene54754.t1 AT3G58190 49.474 95 47 1 73 166 11 105 1.87e-26 102
MS.gene54754.t1 AT3G50510 45.192 104 57 0 70 173 9 112 4.33e-26 100
MS.gene54754.t1 AT3G50510 45.192 104 57 0 70 173 9 112 4.33e-26 100
MS.gene54754.t1 AT5G06080 44.340 106 58 1 72 176 6 111 3.45e-25 97.8
MS.gene54754.t1 AT1G72980 45.263 95 52 0 72 166 12 106 5.54e-24 95.5
MS.gene54754.t1 AT5G35900 41.000 100 59 0 74 173 6 105 4.37e-23 93.2
MS.gene54754.t1 AT3G47870 41.176 102 60 0 72 173 35 136 3.19e-22 93.2
MS.gene54754.t1 AT3G13850 43.011 93 53 0 74 166 37 129 5.23e-22 91.7
MS.gene54754.t1 AT4G22700 37.624 101 61 2 74 173 6 105 1.06e-15 73.2
MS.gene54754.t1 AT1G06280 38.824 85 49 1 73 154 24 108 1.25e-15 73.2
MS.gene54754.t1 AT2G19820 38.202 89 54 1 72 159 11 99 1.05e-14 68.6
MS.gene54754.t1 AT5G15060 42.857 77 40 2 71 144 15 90 8.23e-12 61.2
MS.gene54754.t1 AT1G36000 33.333 87 58 0 73 159 9 95 4.93e-11 58.9

Find 61 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTGCTTGGGAGCCTCTT+TGG 0.215297 1.2:+72651342 MS.gene54754:CDS
ACCATTGCTCATAGAGTCTT+TGG 0.230237 1.2:+72650118 MS.gene54754:CDS
AGGATAAGGGACCCAGTTTA+TGG 0.257729 1.2:+72651095 MS.gene54754:CDS
GCACCTGCACACCCATAAAC+TGG 0.308339 1.2:-72651107 None:intergenic
AACTCATATCAACATGAGTT+TGG 0.323148 1.2:-72651295 None:intergenic
GCACAAGGGCTAACAATAAT+TGG 0.330791 1.2:-72650013 None:intergenic
CACTAGCTCAGCTTGTGCTT+TGG 0.331624 1.2:-72651177 None:intergenic
GCATCTGCTCTCTGAGGTTC+AGG 0.338371 1.2:-72651041 None:intergenic
CAACATCATTAAGTTCTTGC+AGG 0.371270 1.2:+72650147 MS.gene54754:CDS
ACTCATATCAACATGAGTTT+GGG 0.371353 1.2:-72651294 None:intergenic
GTGCCACTAAATTTGCTTGT+TGG 0.373985 1.2:-72651211 None:intergenic
GGTGGAGGAGATGGTGGTTG+AGG 0.390187 1.2:-72649989 None:intergenic
GGAGGAGAATATTGAGAAGC+TGG 0.397244 1.2:-72649956 None:intergenic
CCTGCGACGGAGAATCTTGC+AGG 0.417127 1.2:-72650038 None:intergenic
ATGAAGCAAATGCTAGGATA+AGG 0.419747 1.2:+72651081 MS.gene54754:CDS
CCTGCAAGATTCTCCGTCGC+AGG 0.451176 1.2:+72650038 MS.gene54754:CDS
GTGTGAATGGGAACATGAAT+TGG 0.456087 1.2:-72649860 None:intergenic
GGATAAGGGACCCAGTTTAT+GGG 0.457995 1.2:+72651096 MS.gene54754:CDS
TTGCCAACAAGCAAATTTAG+TGG 0.460480 1.2:+72651208 MS.gene54754:CDS
GACCCAGTTTATGGGTGTGC+AGG 0.487417 1.2:+72651104 MS.gene54754:CDS
GTTTGATAGCATGGTGTGAA+TGG 0.487876 1.2:-72649873 None:intergenic
TTTAGTGGCACTAATTTGCA+TGG 0.489022 1.2:+72651223 MS.gene54754:CDS
AGAATCTTGCAGGCAGCACA+AGG 0.497089 1.2:-72650028 None:intergenic
GGATCAGTTGGAGGAAAATA+CGG 0.507188 1.2:-72650085 None:intergenic
ATAATTGGTGGTGGAGGAGA+TGG 0.508722 1.2:-72649998 None:intergenic
GAATATTGAGAAGCTGGTGA+AGG 0.509511 1.2:-72649950 None:intergenic
TGAAGCAAATGCTAGGATAA+GGG 0.510590 1.2:+72651082 MS.gene54754:CDS
TAGATGAGGAGGAGAAGTAG+TGG 0.517097 1.2:-72649831 None:intergenic
GCTGGTGAAGGGAAAGGTGT+GGG 0.521269 1.2:-72649938 None:intergenic
TGGTGGTTGAGGAGCAGCAG+AGG 0.528834 1.2:-72649978 None:intergenic
AAAGGTGTGGGAGATTGTAA+AGG 0.533363 1.2:-72649926 None:intergenic
TGAGAAGCTGGTGAAGGGAA+AGG 0.534082 1.2:-72649944 None:intergenic
TGTGAATGGGAACATGAATT+GGG 0.535599 1.2:-72649859 None:intergenic
ATTGGGATTGGTAGATGAGG+AGG 0.537299 1.2:-72649842 None:intergenic
AGCTGGTGAAGGGAAAGGTG+TGG 0.541852 1.2:-72649939 None:intergenic
AATTAATTTCATGTCCAAAG+AGG 0.542951 1.2:-72651356 None:intergenic
ATGGGAACATGAATTGGGAT+TGG 0.544335 1.2:-72649854 None:intergenic
TTAATCATCATGTCATGGTC+TGG 0.552049 1.2:-72651266 None:intergenic
GGAGGTGAAGAAGATGAAGA+TGG 0.556111 1.2:-72649905 None:intergenic
GGTGTGGGAGATTGTAAAGG+AGG 0.557869 1.2:-72649923 None:intergenic
CACCTGCACACCCATAAACT+GGG 0.560148 1.2:-72651106 None:intergenic
ATTGGTGGTGGAGGAGATGG+TGG 0.560667 1.2:-72649995 None:intergenic
GGGCTAACAATAATTGGTGG+TGG 0.570890 1.2:-72650007 None:intergenic
TGTAGTAGAGTTTGATAGCA+TGG 0.578297 1.2:-72649882 None:intergenic
TGGTTTATGAAGCAAATGCT+AGG 0.582803 1.2:+72651075 MS.gene54754:CDS
TGAATTGGGATTGGTAGATG+AGG 0.583122 1.2:-72649845 None:intergenic
TTTGATAGCATGGTGTGAAT+GGG 0.585620 1.2:-72649872 None:intergenic
TCCAAAGACTCTATGAGCAA+TGG 0.589978 1.2:-72650119 None:intergenic
CTAACAATAATTGGTGGTGG+AGG 0.590976 1.2:-72650004 None:intergenic
AACTTGAGTGGATCAGTTGG+AGG 0.595286 1.2:-72650094 None:intergenic
CTTACTGCATCTGCTCTCTG+AGG 0.598505 1.2:-72651047 None:intergenic
GTGAACTTGAGTGGATCAGT+TGG 0.601167 1.2:-72650097 None:intergenic
AATATTGAGAAGCTGGTGAA+GGG 0.602247 1.2:-72649949 None:intergenic
GCTGATTAATCATCATGTCA+TGG 0.608852 1.2:-72651271 None:intergenic
GGTTGAGGAGCAGCAGAGGG+AGG 0.611544 1.2:-72649974 None:intergenic
CTCATATCAACATGAGTTTG+GGG 0.613857 1.2:-72651293 None:intergenic
GGTGGTTGAGGAGCAGCAGA+GGG 0.614036 1.2:-72649977 None:intergenic
AGCAGATGCAGTAAGCAGCA+TGG 0.619103 1.2:+72651055 MS.gene54754:CDS
TGAGCAATGGTGAACTTGAG+TGG 0.624171 1.2:-72650106 None:intergenic
CAAGGGCTAACAATAATTGG+TGG 0.638801 1.2:-72650010 None:intergenic
GAATCTTGCAGGCAGCACAA+GGG 0.696277 1.2:-72650027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTTTTTTCAACATAT+TGG + chr1.2:72650237-72650256 MS.gene54754:intron 10.0%
!! AATAAAATGTACTATAAGTT+AGG - chr1.2:72650842-72650861 None:intergenic 15.0%
!! AATAAATTTAGAGAAAATCT+TGG + chr1.2:72650871-72650890 MS.gene54754:intron 15.0%
!! TAGTTATAATAAGTGAAAAA+TGG + chr1.2:72650894-72650913 MS.gene54754:intron 15.0%
!!! TAAAATTCCATTAATTTTTC+AGG + chr1.2:72651013-72651032 MS.gene54754:intron 15.0%
!! ATCACAAGTCAAAAATAATT+AGG + chr1.2:72650702-72650721 MS.gene54754:intron 20.0%
!! CATGAAAAAAATAAAAGCTA+TGG + chr1.2:72650588-72650607 MS.gene54754:intron 20.0%
!! TGTAAAAGATAAAAAGTTAG+TGG - chr1.2:72650570-72650589 None:intergenic 20.0%
!! TGTATGATCTTAAAACAAAT+TGG + chr1.2:72650269-72650288 MS.gene54754:intron 20.0%
!!! TATTTTAGTAATGTGCAAAT+TGG - chr1.2:72650222-72650241 None:intergenic 20.0%
! AAACTTATGTGCATTTGTTT+AGG - chr1.2:72650931-72650950 None:intergenic 25.0%
! AAAGATAAAAAGTTAGTGGT+TGG - chr1.2:72650566-72650585 None:intergenic 25.0%
! AATGAAAAGATTCCAAAATG+TGG - chr1.2:72650731-72650750 None:intergenic 25.0%
! ATGAAAAGATTCCAAAATGT+GGG - chr1.2:72650730-72650749 None:intergenic 25.0%
! ATTAAGCCATGATTTGAAAA+TGG - chr1.2:72650957-72650976 None:intergenic 25.0%
! TTCATTATGTCTAACGATTA+TGG + chr1.2:72650745-72650764 MS.gene54754:intron 25.0%
!! AATTGTCTCATAACAGTTTT+AGG + chr1.2:72650389-72650408 MS.gene54754:intron 25.0%
!! ATTGTCTCATAACAGTTTTA+GGG + chr1.2:72650390-72650409 MS.gene54754:intron 25.0%
!! TTTTGTCCATTTTCAAATCA+TGG + chr1.2:72650948-72650967 MS.gene54754:intron 25.0%
!!! ACATGATGATTTTTTCTTGT+TGG - chr1.2:72650193-72650212 None:intergenic 25.0%
!!! TTTTAGAGGACAACAATTTT+GGG + chr1.2:72651321-72651340 MS.gene54754:CDS 25.0%
!!! TTTTTAGAGGACAACAATTT+TGG + chr1.2:72651320-72651339 MS.gene54754:CDS 25.0%
AACTCATATCAACATGAGTT+TGG - chr1.2:72651298-72651317 None:intergenic 30.0%
AAGATATAGTGGTTACACTA+TGG - chr1.2:72650682-72650701 None:intergenic 30.0%
ACTCATATCAACATGAGTTT+GGG - chr1.2:72651297-72651316 None:intergenic 30.0%
GGTAGTTCCTGAAAAATTAA+TGG - chr1.2:72651023-72651042 None:intergenic 30.0%
TGGATGGAAAAGATAACAAA+AGG + chr1.2:72650442-72650461 MS.gene54754:intron 30.0%
! TGACTTGTGATAAGATATAG+TGG - chr1.2:72650693-72650712 None:intergenic 30.0%
!! AGTTTTTCACTTTGGAAACT+TGG - chr1.2:72650789-72650808 None:intergenic 30.0%
!! TTGTCTCATAACAGTTTTAG+GGG + chr1.2:72650391-72650410 MS.gene54754:intron 30.0%
!! TTTTAGGGGAACAAAATCAT+GGG + chr1.2:72650405-72650424 MS.gene54754:intron 30.0%
!!! TGATATGAGTTGCTTTTTAG+AGG + chr1.2:72651307-72651326 MS.gene54754:CDS 30.0%
AATATTGAGAAGCTGGTGAA+GGG - chr1.2:72649952-72649971 None:intergenic 35.0%
ATGAAGCAAATGCTAGGATA+AGG + chr1.2:72651081-72651100 MS.gene54754:CDS 35.0%
CAACATCATTAAGTTCTTGC+AGG + chr1.2:72650147-72650166 MS.gene54754:CDS 35.0%
CTCATATCAACATGAGTTTG+GGG - chr1.2:72651296-72651315 None:intergenic 35.0%
GCTGATTAATCATCATGTCA+TGG - chr1.2:72651274-72651293 None:intergenic 35.0%
GTCTAACGATTATGGTCAAT+GGG + chr1.2:72650753-72650772 MS.gene54754:intron 35.0%
TCTAACGATTATGGTCAATG+GGG + chr1.2:72650754-72650773 MS.gene54754:intron 35.0%
TGAAGCAAATGCTAGGATAA+GGG + chr1.2:72651082-72651101 MS.gene54754:CDS 35.0%
TGGTTTATGAAGCAAATGCT+AGG + chr1.2:72651075-72651094 MS.gene54754:CDS 35.0%
TGTCTAACGATTATGGTCAA+TGG + chr1.2:72650752-72650771 MS.gene54754:intron 35.0%
TGTGAATGGGAACATGAATT+GGG - chr1.2:72649862-72649881 None:intergenic 35.0%
TTAATCATCATGTCATGGTC+TGG - chr1.2:72651269-72651288 None:intergenic 35.0%
TTGCCAACAAGCAAATTTAG+TGG + chr1.2:72651208-72651227 MS.gene54754:CDS 35.0%
! AAAGGAGCATACCTAATTGA+TGG + chr1.2:72650460-72650479 MS.gene54754:intron 35.0%
! AAGGAGCATACCTAATTGAT+GGG + chr1.2:72650461-72650480 MS.gene54754:intron 35.0%
! ATAATTAGGTGCCCACATTT+TGG + chr1.2:72650716-72650735 MS.gene54754:intron 35.0%
! TGCACTCAAGTTTTTCACTT+TGG - chr1.2:72650797-72650816 None:intergenic 35.0%
! TGTAGTAGAGTTTGATAGCA+TGG - chr1.2:72649885-72649904 None:intergenic 35.0%
! TTTAGTGGCACTAATTTGCA+TGG + chr1.2:72651223-72651242 MS.gene54754:CDS 35.0%
! TTTGATAGCATGGTGTGAAT+GGG - chr1.2:72649875-72649894 None:intergenic 35.0%
!! ATGCATAGCTTTGATGTGTT+AGG - chr1.2:72650514-72650533 None:intergenic 35.0%
!!! GTTTTAGGGGAACAAAATCA+TGG + chr1.2:72650404-72650423 MS.gene54754:intron 35.0%
ACCATTGCTCATAGAGTCTT+TGG + chr1.2:72650118-72650137 MS.gene54754:CDS 40.0%
ACTGGTCATAGTCACAAACA+AGG - chr1.2:72650314-72650333 None:intergenic 40.0%
ATCATGCATGCCCATCAATT+AGG - chr1.2:72650474-72650493 None:intergenic 40.0%
ATGGGAACATGAATTGGGAT+TGG - chr1.2:72649857-72649876 None:intergenic 40.0%
CAAGGGCTAACAATAATTGG+TGG - chr1.2:72650013-72650032 None:intergenic 40.0%
GAATATTGAGAAGCTGGTGA+AGG - chr1.2:72649953-72649972 None:intergenic 40.0%
GCACAAGGGCTAACAATAAT+TGG - chr1.2:72650016-72650035 None:intergenic 40.0%
GGATCAGTTGGAGGAAAATA+CGG - chr1.2:72650088-72650107 None:intergenic 40.0%
GTGTGAATGGGAACATGAAT+TGG - chr1.2:72649863-72649882 None:intergenic 40.0%
TCCAAAGACTCTATGAGCAA+TGG - chr1.2:72650122-72650141 None:intergenic 40.0%
! AAAGGTGTGGGAGATTGTAA+AGG - chr1.2:72649929-72649948 None:intergenic 40.0%
! GGGAACAAAATCATGGGTTT+TGG + chr1.2:72650411-72650430 MS.gene54754:intron 40.0%
! GTGCCACTAAATTTGCTTGT+TGG - chr1.2:72651214-72651233 None:intergenic 40.0%
! GTTTGATAGCATGGTGTGAA+TGG - chr1.2:72649876-72649895 None:intergenic 40.0%
! TGAATTGGGATTGGTAGATG+AGG - chr1.2:72649848-72649867 None:intergenic 40.0%
!! ACAACAATTTTGGGTCTGCT+TGG + chr1.2:72651330-72651349 MS.gene54754:CDS 40.0%
!! CAACAATTTTGGGTCTGCTT+GGG + chr1.2:72651331-72651350 MS.gene54754:CDS 40.0%
!! CATGGGTTTTGGTCAAAAAG+TGG + chr1.2:72650422-72650441 MS.gene54754:intron 40.0%
!! CTAACAATAATTGGTGGTGG+AGG - chr1.2:72650007-72650026 None:intergenic 40.0%
!! GGTTTTGGTCAAAAAGTGGA+TGG + chr1.2:72650426-72650445 MS.gene54754:intron 40.0%
AACTTGAGTGGATCAGTTGG+AGG - chr1.2:72650097-72650116 None:intergenic 45.0%
AAGAGAATGTCATGCCTCGA+AGG - chr1.2:72650346-72650365 None:intergenic 45.0%
AGGATAAGGGACCCAGTTTA+TGG + chr1.2:72651095-72651114 MS.gene54754:CDS 45.0%
ATAGTGGTTACACTATGGCC+AGG - chr1.2:72650677-72650696 None:intergenic 45.0%
GGAGGAGAATATTGAGAAGC+TGG - chr1.2:72649959-72649978 None:intergenic 45.0%
GGAGGTGAAGAAGATGAAGA+TGG - chr1.2:72649908-72649927 None:intergenic 45.0%
GGATAAGGGACCCAGTTTAT+GGG + chr1.2:72651096-72651115 MS.gene54754:CDS 45.0%
GGGCTAACAATAATTGGTGG+TGG - chr1.2:72650010-72650029 None:intergenic 45.0%
GTGAACTTGAGTGGATCAGT+TGG - chr1.2:72650100-72650119 None:intergenic 45.0%
TAGATGAGGAGGAGAAGTAG+TGG - chr1.2:72649834-72649853 None:intergenic 45.0%
TAGTGGTTACACTATGGCCA+GGG - chr1.2:72650676-72650695 None:intergenic 45.0%
TGAGCAATGGTGAACTTGAG+TGG - chr1.2:72650109-72650128 None:intergenic 45.0%
! ACTTTTCAACACACCTGCGA+CGG - chr1.2:72650054-72650073 None:intergenic 45.0%
! ATTGGGATTGGTAGATGAGG+AGG - chr1.2:72649845-72649864 None:intergenic 45.0%
!! ATAATTGGTGGTGGAGGAGA+TGG - chr1.2:72650001-72650020 None:intergenic 45.0%
AGAATCTTGCAGGCAGCACA+AGG - chr1.2:72650031-72650050 None:intergenic 50.0%
AGCAGATGCAGTAAGCAGCA+TGG + chr1.2:72651055-72651074 MS.gene54754:CDS 50.0%
AGTGGTTACACTATGGCCAG+GGG - chr1.2:72650675-72650694 None:intergenic 50.0%
CACCTGCACACCCATAAACT+GGG - chr1.2:72651109-72651128 None:intergenic 50.0%
CACTAGCTCAGCTTGTGCTT+TGG - chr1.2:72651180-72651199 None:intergenic 50.0%
CCAGTTCATTCACACCTTCG+AGG + chr1.2:72650329-72650348 MS.gene54754:intron 50.0%
CTTACTGCATCTGCTCTCTG+AGG - chr1.2:72651050-72651069 None:intergenic 50.0%
CTTTCTCTCTTTAGTCCCCC+TGG + chr1.2:72650656-72650675 MS.gene54754:intron 50.0%
GAATCTTGCAGGCAGCACAA+GGG - chr1.2:72650030-72650049 None:intergenic 50.0%
GGTGTGGGAGATTGTAAAGG+AGG - chr1.2:72649926-72649945 None:intergenic 50.0%
TGAGAAGCTGGTGAAGGGAA+AGG - chr1.2:72649947-72649966 None:intergenic 50.0%
! CCTCGAAGGTGTGAATGAAC+TGG - chr1.2:72650332-72650351 None:intergenic 50.0%
AGCTGGTGAAGGGAAAGGTG+TGG - chr1.2:72649942-72649961 None:intergenic 55.0%
GACCCAGTTTATGGGTGTGC+AGG + chr1.2:72651104-72651123 MS.gene54754:CDS 55.0%
GCACCTGCACACCCATAAAC+TGG - chr1.2:72651110-72651129 None:intergenic 55.0%
GTGGTTACACTATGGCCAGG+GGG - chr1.2:72650674-72650693 None:intergenic 55.0%
! GCTGGTGAAGGGAAAGGTGT+GGG - chr1.2:72649941-72649960 None:intergenic 55.0%
!! ATTGGTGGTGGAGGAGATGG+TGG - chr1.2:72649998-72650017 None:intergenic 55.0%
!! GCATCTGCTCTCTGAGGTTC+AGG - chr1.2:72651044-72651063 None:intergenic 55.0%
CCTGCAAGATTCTCCGTCGC+AGG + chr1.2:72650038-72650057 MS.gene54754:CDS 60.0%
CCTGCGACGGAGAATCTTGC+AGG - chr1.2:72650041-72650060 None:intergenic 60.0%
GGTCTGCTTGGGAGCCTCTT+TGG + chr1.2:72651342-72651361 MS.gene54754:CDS 60.0%
GGTGGTTGAGGAGCAGCAGA+GGG - chr1.2:72649980-72649999 None:intergenic 60.0%
TGGTGGTTGAGGAGCAGCAG+AGG - chr1.2:72649981-72650000 None:intergenic 60.0%
! GGTGGAGGAGATGGTGGTTG+AGG - chr1.2:72649992-72650011 None:intergenic 60.0%
GGTTGAGGAGCAGCAGAGGG+AGG - chr1.2:72649977-72649996 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 72649812 72651370 72649812 ID=MS.gene54754
chr1.2 mRNA 72649812 72651370 72649812 ID=MS.gene54754.t1;Parent=MS.gene54754
chr1.2 exon 72649812 72650168 72649812 ID=MS.gene54754.t1.exon1;Parent=MS.gene54754.t1
chr1.2 CDS 72649812 72650168 72649812 ID=cds.MS.gene54754.t1;Parent=MS.gene54754.t1
chr1.2 exon 72651035 72651370 72651035 ID=MS.gene54754.t1.exon2;Parent=MS.gene54754.t1
chr1.2 CDS 72651035 72651370 72651035 ID=cds.MS.gene54754.t1;Parent=MS.gene54754.t1
Gene Sequence

>MS.gene54754

ATGGAACACAAAATGAAAACCACTACTTCTCCTCCTCATCTACCAATCCCAATTCATGTTCCCATTCACACCATGCTATCAAACTCTACTACACCATCTTCATCTTCTTCACCTCCTTTACAATCTCCCACACCTTTCCCTTCACCAGCTTCTCAATATTCTCCTCCCTCTGCTGCTCCTCAACCACCATCTCCTCCACCACCAATTATTGTTAGCCCTTGTGCTGCCTGCAAGATTCTCCGTCGCAGGTGTGTTGAAAAGTGTGTGTTAGCTCCGTATTTTCCTCCAACTGATCCACTCAAGTTCACCATTGCTCATAGAGTCTTTGGAGCTAGCAACATCATTAAGTTCTTGCAGGTAACATACATTCATAATTCACCAACAAGAAAAAATCATCATGTTACAAACCAATTTGCACATTACTAAAATATTTTTTTCAACATATTGGACATTTATATGTATGATCTTAAAACAAATTGGTGCGATTCTATTGTTGTTGCCTTGTTTGTGACTATGACCAGTTCATTCACACCTTCGAGGCATGACATTCTCTTGAAAAACAACAAAAAATTAGTTGAATTGTCTCATAACAGTTTTAGGGGAACAAAATCATGGGTTTTGGTCAAAAAGTGGATGGAAAAGATAACAAAAGGAGCATACCTAATTGATGGGCATGCATGATGTGTTGTTTTCTAACTTCCTAACACATCAAAGCTATGCATAATTCTCTCTTCTTTACATTCTACATCAACCAACCACTAACTTTTTATCTTTTACATGAAAAAAATAAAAGCTATGGACAAGTCAAAATAACATTTTGCATATTGCTTGTTTATCTTAATTTCTTTCTCTCTTTAGTCCCCCTGGCCATAGTGTAACCACTATATCTTATCACAAGTCAAAAATAATTAGGTGCCCACATTTTGGAATCTTTTCATTATGTCTAACGATTATGGTCAATGGGGCATGAAGTACCAAGTTTCCAAAGTGAAAAACTTGAGTGCATATCTTTAGTCTTCGTATTCTTCCTAACTTATAGTACATTTTATTTATAGTTATAATAAATTTAGAGAAAATCTTGGTAGTTATAATAAGTGAAAAATGGTACGAAAAGTACCTAAACAAATGCACATAAGTTTTGTCCATTTTCAAATCATGGCTTAATTTGTAATTAAGAGTTTTAGTTAGTAAATAACAAAAATAAAATTCCATTAATTTTTCAGGAACTACCTGAACCTCAGAGAGCAGATGCAGTAAGCAGCATGGTTTATGAAGCAAATGCTAGGATAAGGGACCCAGTTTATGGGTGTGCAGGTGCAATATGTCAACTTCAAAAACAAGTTAGTGAGTTACAAACACAGCTTGCCAAAGCACAAGCTGAGCTAGTGAACATGCATTGCCAACAAGCAAATTTAGTGGCACTAATTTGCATGGAAATGAGTACTCAGTCATCACCAGACCATGACATGATGATTAATCAGCCCCAAACTCATGTTGATATGAGTTGCTTTTTAGAGGACAACAATTTTGGGTCTGCTTGGGAGCCTCTTTGGACATGA
Protein sequence

>MS.gene54754.t1

MEHKMKTTTSPPHLPIPIHVPIHTMLSNSTTPSSSSSPPLQSPTPFPSPASQYSPPSAAPQPPSPPPPIIVSPCAACKILRRRCVEKCVLAPYFPPTDPLKFTIAHRVFGASNIIKFLQELPEPQRADAVSSMVYEANARIRDPVYGCAGAICQLQKQVSELQTQLAKAQAELVNMHCQQANLVALICMEMSTQSSPDHDMMINQPQTHVDMSCFLEDNNFGSAWEPLWT