Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54941.t1 | XP_024636235.1 | 93.9 | 214 | 13 | 0 | 1 | 214 | 30 | 243 | 3.00E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54941.t1 | A0A396I2K7 | 65.7 | 216 | 68 | 1 | 1 | 210 | 30 | 245 | 2.4e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene54941.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54941.t1 | MTR_4g036615 | 80.769 | 104 | 20 | 0 | 49 | 152 | 1 | 104 | 4.90e-57 | 176 |
| MS.gene54941.t1 | MTR_4g100860 | 61.017 | 59 | 21 | 1 | 144 | 200 | 210 | 268 | 3.34e-15 | 73.2 |
| MS.gene54941.t1 | MTR_0160s0030 | 40.964 | 83 | 39 | 1 | 139 | 211 | 237 | 319 | 9.24e-15 | 72.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54941.t1 | AT3G60580 | 42.353 | 85 | 34 | 2 | 131 | 200 | 162 | 246 | 8.33e-15 | 72.0 |
| MS.gene54941.t1 | AT2G45120 | 46.154 | 78 | 29 | 2 | 138 | 202 | 190 | 267 | 4.00e-14 | 70.1 |
| MS.gene54941.t1 | AT1G02030 | 45.570 | 79 | 30 | 2 | 142 | 207 | 160 | 238 | 9.30e-14 | 68.6 |
| MS.gene54941.t1 | AT5G61470 | 37.805 | 82 | 39 | 2 | 130 | 200 | 216 | 296 | 1.10e-12 | 66.2 |
Find 42 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAACACTTTGTTGCAATT+TGG | 0.166386 | 4.3:-63401686 | None:intergenic |
| TTCTGTGTTTGTTGTATTTC+GGG | 0.187551 | 4.3:-63401257 | None:intergenic |
| ATTCTGTGTTTGTTGTATTT+CGG | 0.237824 | 4.3:-63401258 | None:intergenic |
| TACTCAAGCTGTAGACAATT+TGG | 0.312612 | 4.3:+63401184 | MS.gene54941:CDS |
| AATTGGATCTCTTGCGGGTT+GGG | 0.313326 | 4.3:-63401361 | None:intergenic |
| TGAACACTTTGTTGCAATTT+GGG | 0.315013 | 4.3:-63401685 | None:intergenic |
| TGAGTATTAGTTTCAAGCTT+TGG | 0.337317 | 4.3:-63401167 | None:intergenic |
| GCCGCAATTGGATCTCTTGC+GGG | 0.342035 | 4.3:-63401366 | None:intergenic |
| CAATTGGATCTCTTGCGGGT+TGG | 0.402481 | 4.3:-63401362 | None:intergenic |
| ATTAAGTTCGCGGCCGCAAT+TGG | 0.437633 | 4.3:-63401378 | None:intergenic |
| GTTTCAAGCTTTGGAGCAAT+CGG | 0.454553 | 4.3:-63401158 | None:intergenic |
| TTCCGATCTTATCAGGCTCT+TGG | 0.457389 | 4.3:+63401596 | MS.gene54941:CDS |
| TACAGAATCACAAATCAATA+CGG | 0.462697 | 4.3:-63401782 | None:intergenic |
| TTGCAATTTGGGCAGTGAAA+AGG | 0.463868 | 4.3:-63401674 | None:intergenic |
| CGATCTTATCAGGCTCTTGG+TGG | 0.468794 | 4.3:+63401599 | MS.gene54941:CDS |
| TTCAAGTCTGCTCAGGCACT+CGG | 0.500253 | 4.3:+63401704 | MS.gene54941:CDS |
| CCCCATAACATCAAAACACC+TGG | 0.500612 | 4.3:-63401434 | None:intergenic |
| GGCCGCAATTGGATCTCTTG+CGG | 0.503301 | 4.3:-63401367 | None:intergenic |
| TCTGTTTGAGTTTGCAGAGG+AGG | 0.504442 | 4.3:-63401309 | None:intergenic |
| AACGATGGCGAAAGCAGTAC+AGG | 0.507873 | 4.3:+63401512 | MS.gene54941:CDS |
| GCACCTCTCACAGTTGAACC+TGG | 0.509618 | 4.3:-63401564 | None:intergenic |
| CAGCCGCAGCATTAAGTTCG+CGG | 0.512384 | 4.3:-63401388 | None:intergenic |
| TTCTCTGTTTGAGTTTGCAG+AGG | 0.512537 | 4.3:-63401312 | None:intergenic |
| GAAGATATGTCAAGAGATCC+AGG | 0.516117 | 4.3:+63401416 | MS.gene54941:CDS |
| ACTTTGAAATTCCTTAGCAC+TGG | 0.521740 | 4.3:-63401750 | None:intergenic |
| CAAAGTGTTCAAGTCTGCTC+AGG | 0.524975 | 4.3:+63401697 | MS.gene54941:CDS |
| CACAAGCAGACTCTGAAGCC+AGG | 0.539423 | 4.3:+63401546 | MS.gene54941:CDS |
| GTGTTTGTTGTATTTCGGGA+AGG | 0.552910 | 4.3:-63401253 | None:intergenic |
| GATCTTATCAGGCTCTTGGT+GGG | 0.571526 | 4.3:+63401600 | MS.gene54941:CDS |
| GGTACACTCTTCCAGTGCTA+AGG | 0.575791 | 4.3:+63401739 | MS.gene54941:CDS |
| CACCAAGAGCCTGATAAGAT+CGG | 0.582662 | 4.3:-63401598 | None:intergenic |
| AGGCACTCGGTGGACACAAG+AGG | 0.606213 | 4.3:+63401717 | MS.gene54941:CDS |
| ACAGAATCACAAATCAATAC+GGG | 0.613334 | 4.3:-63401781 | None:intergenic |
| TACTGCAAAAGAGACAATGA+TGG | 0.621835 | 4.3:+63401632 | MS.gene54941:CDS |
| AAGCCAGGTTCAACTGTGAG+AGG | 0.622627 | 4.3:+63401561 | MS.gene54941:CDS |
| ATCACAAATCAATACGGGTG+TGG | 0.631203 | 4.3:-63401776 | None:intergenic |
| GGCACTCGGTGGACACAAGA+GGG | 0.632635 | 4.3:+63401718 | MS.gene54941:CDS |
| CGGCCGCGAACTTAATGCTG+CGG | 0.660556 | 4.3:+63401385 | MS.gene54941:CDS |
| AGGTTCAACTGTGAGAGGTG+CGG | 0.663022 | 4.3:+63401566 | MS.gene54941:CDS |
| TTCGGGAAGGCAAGTCTGTG+AGG | 0.669270 | 4.3:-63401240 | None:intergenic |
| AAGTCTGCTCAGGCACTCGG+TGG | 0.670340 | 4.3:+63401707 | MS.gene54941:CDS |
| ACCCGCAAGAGATCCAATTG+CGG | 0.686880 | 4.3:+63401365 | MS.gene54941:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GATCAAGAAACAAAAAATGA+GGG | + | chr4.3:63401487-63401506 | MS.gene54941:CDS | 25.0% |
| !!! | ATTCTGTGTTTGTTGTATTT+CGG | - | chr4.3:63401261-63401280 | None:intergenic | 25.0% |
| AAAAATGTTCCGATCTTATC+AGG | + | chr4.3:63401589-63401608 | MS.gene54941:CDS | 30.0% | |
| GGATCAAGAAACAAAAAATG+AGG | + | chr4.3:63401486-63401505 | MS.gene54941:CDS | 30.0% | |
| TGAACACTTTGTTGCAATTT+GGG | - | chr4.3:63401688-63401707 | None:intergenic | 30.0% | |
| TTACAAAGATGCGCAAAAAA+GGG | + | chr4.3:63401460-63401479 | MS.gene54941:CDS | 30.0% | |
| TTGAACACTTTGTTGCAATT+TGG | - | chr4.3:63401689-63401708 | None:intergenic | 30.0% | |
| TTTACAAAGATGCGCAAAAA+AGG | + | chr4.3:63401459-63401478 | MS.gene54941:CDS | 30.0% | |
| ! | TGAGTATTAGTTTCAAGCTT+TGG | - | chr4.3:63401170-63401189 | None:intergenic | 30.0% |
| !! | AAGCAAATTGATTTTGACGA+TGG | - | chr4.3:63401220-63401239 | None:intergenic | 30.0% |
| !! | AGCAAATTGATTTTGACGAT+GGG | - | chr4.3:63401219-63401238 | None:intergenic | 30.0% |
| !! | TTCTGTGTTTGTTGTATTTC+GGG | - | chr4.3:63401260-63401279 | None:intergenic | 30.0% |
| CAAAAAATGAGGGAAAACGA+TGG | + | chr4.3:63401497-63401516 | MS.gene54941:CDS | 35.0% | |
| TACTCAAGCTGTAGACAATT+TGG | + | chr4.3:63401184-63401203 | MS.gene54941:CDS | 35.0% | |
| TACTGCAAAAGAGACAATGA+TGG | + | chr4.3:63401632-63401651 | MS.gene54941:CDS | 35.0% | |
| !! | ACTTTGAAATTCCTTAGCAC+TGG | - | chr4.3:63401753-63401772 | None:intergenic | 35.0% |
| !!! | ATCCAGGTGTTTTGATGTTA+TGG | + | chr4.3:63401432-63401451 | MS.gene54941:CDS | 35.0% |
| !!! | ATTTTGACGATGGGTTGAAT+CGG | - | chr4.3:63401210-63401229 | None:intergenic | 35.0% |
| !!! | CGAAGTTTTGTTTCATGGAT+AGG | - | chr4.3:63401286-63401305 | None:intergenic | 35.0% |
| !!! | TCCAGGTGTTTTGATGTTAT+GGG | + | chr4.3:63401433-63401452 | MS.gene54941:CDS | 35.0% |
| AAAGATGCGCAAAAAAGGGA+AGG | + | chr4.3:63401464-63401483 | MS.gene54941:CDS | 40.0% | |
| AAGATGCGCAAAAAAGGGAA+GGG | + | chr4.3:63401465-63401484 | MS.gene54941:CDS | 40.0% | |
| GAAGATATGTCAAGAGATCC+AGG | + | chr4.3:63401416-63401435 | MS.gene54941:CDS | 40.0% | |
| TTGCAATTTGGGCAGTGAAA+AGG | - | chr4.3:63401677-63401696 | None:intergenic | 40.0% | |
| ! | TTCTCTGTTTGAGTTTGCAG+AGG | - | chr4.3:63401315-63401334 | None:intergenic | 40.0% |
| !! | GTGTTTGTTGTATTTCGGGA+AGG | - | chr4.3:63401256-63401275 | None:intergenic | 40.0% |
| !! | GTTTCAAGCTTTGGAGCAAT+CGG | - | chr4.3:63401161-63401180 | None:intergenic | 40.0% |
| !!! | CCAGGTGTTTTGATGTTATG+GGG | + | chr4.3:63401434-63401453 | MS.gene54941:CDS | 40.0% |
| AATTGGATCTCTTGCGGGTT+GGG | - | chr4.3:63401364-63401383 | None:intergenic | 45.0% | |
| CACCAAGAGCCTGATAAGAT+CGG | - | chr4.3:63401601-63401620 | None:intergenic | 45.0% | |
| CCCCATAACATCAAAACACC+TGG | - | chr4.3:63401437-63401456 | None:intergenic | 45.0% | |
| GATCTTATCAGGCTCTTGGT+GGG | + | chr4.3:63401600-63401619 | MS.gene54941:CDS | 45.0% | |
| TTCCGATCTTATCAGGCTCT+TGG | + | chr4.3:63401596-63401615 | MS.gene54941:CDS | 45.0% | |
| ! | CAAAGTGTTCAAGTCTGCTC+AGG | + | chr4.3:63401697-63401716 | MS.gene54941:CDS | 45.0% |
| ! | TCTGTTTGAGTTTGCAGAGG+AGG | - | chr4.3:63401312-63401331 | None:intergenic | 45.0% |
| !!! | GGCAGCGAAGTTTTGTTTCA+TGG | - | chr4.3:63401291-63401310 | None:intergenic | 45.0% |
| AACGATGGCGAAAGCAGTAC+AGG | + | chr4.3:63401512-63401531 | MS.gene54941:CDS | 50.0% | |
| AAGCCAGGTTCAACTGTGAG+AGG | + | chr4.3:63401561-63401580 | MS.gene54941:CDS | 50.0% | |
| ACCCGCAAGAGATCCAATTG+CGG | + | chr4.3:63401365-63401384 | MS.gene54941:CDS | 50.0% | |
| AGGTTCAACTGTGAGAGGTG+CGG | + | chr4.3:63401566-63401585 | MS.gene54941:CDS | 50.0% | |
| ATTAAGTTCGCGGCCGCAAT+TGG | - | chr4.3:63401381-63401400 | None:intergenic | 50.0% | |
| CAATTGGATCTCTTGCGGGT+TGG | - | chr4.3:63401365-63401384 | None:intergenic | 50.0% | |
| CGATCTTATCAGGCTCTTGG+TGG | + | chr4.3:63401599-63401618 | MS.gene54941:CDS | 50.0% | |
| GGTACACTCTTCCAGTGCTA+AGG | + | chr4.3:63401739-63401758 | MS.gene54941:CDS | 50.0% | |
| TTCAAGTCTGCTCAGGCACT+CGG | + | chr4.3:63401704-63401723 | MS.gene54941:CDS | 50.0% | |
| !!! | TCTTGCGGGTTGGGTTTTTC+AGG | - | chr4.3:63401355-63401374 | None:intergenic | 50.0% |
| CAGCCGCAGCATTAAGTTCG+CGG | - | chr4.3:63401391-63401410 | None:intergenic | 55.0% | |
| GCACCTCTCACAGTTGAACC+TGG | - | chr4.3:63401567-63401586 | None:intergenic | 55.0% | |
| GCCGCAATTGGATCTCTTGC+GGG | - | chr4.3:63401369-63401388 | None:intergenic | 55.0% | |
| GGCCGCAATTGGATCTCTTG+CGG | - | chr4.3:63401370-63401389 | None:intergenic | 55.0% | |
| TTCGGGAAGGCAAGTCTGTG+AGG | - | chr4.3:63401243-63401262 | None:intergenic | 55.0% | |
| !! | CACAAGCAGACTCTGAAGCC+AGG | + | chr4.3:63401546-63401565 | MS.gene54941:CDS | 55.0% |
| !!! | GCGGGTTGGGTTTTTCAGGT+TGG | - | chr4.3:63401351-63401370 | None:intergenic | 55.0% |
| AAGTCTGCTCAGGCACTCGG+TGG | + | chr4.3:63401707-63401726 | MS.gene54941:CDS | 60.0% | |
| CGGCCGCGAACTTAATGCTG+CGG | + | chr4.3:63401385-63401404 | MS.gene54941:CDS | 60.0% | |
| ! | AGGCACTCGGTGGACACAAG+AGG | + | chr4.3:63401717-63401736 | MS.gene54941:CDS | 60.0% |
| ! | GGCACTCGGTGGACACAAGA+GGG | + | chr4.3:63401718-63401737 | MS.gene54941:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 63401146 | 63401790 | 63401146 | ID=MS.gene54941 |
| chr4.3 | mRNA | 63401146 | 63401790 | 63401146 | ID=MS.gene54941.t1;Parent=MS.gene54941 |
| chr4.3 | exon | 63401146 | 63401790 | 63401146 | ID=MS.gene54941.t1.exon1;Parent=MS.gene54941.t1 |
| chr4.3 | CDS | 63401146 | 63401790 | 63401146 | ID=cds.MS.gene54941.t1;Parent=MS.gene54941.t1 |
| Gene Sequence |
| Protein sequence |
