AlfalfaGEDB | MS.gene54947

M. sativa cultivar XinJiangDaYe / MS.gene54947

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54947.t1	XP_003624493.1	83.3	78	13	0	1	78	2	79	4.00E-28	133.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54947.t1	Q9LFQ3	56.9	65	26	2	10	74	51	113	1.1e-10	67.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54947.t1	G7KX87	83.3	78	13	0	1	78	2	79	2.9e-28	133.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene54947.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54947.t1	MTR_7g083950	83.333	78	13	0	1	78	2	79	6.96e-40	135
MS.gene54947.t1	MTR_5g073430	71.795	78	22	0	1	78	1	78	3.84e-35	115
MS.gene54947.t1	MTR_3g006125	56.522	69	27	2	11	79	89	154	7.78e-14	64.3
MS.gene54947.t1	MTR_3g006125	54.688	64	28	1	11	74	180	242	3.54e-12	59.7
MS.gene54947.t1	MTR_6g008970	56.923	65	26	2	10	74	62	124	8.58e-14	65.1
MS.gene54947.t1	MTR_3g006185	55.072	69	28	2	11	79	8	73	9.46e-14	62.8
MS.gene54947.t1	MTR_3g006185	54.688	64	28	1	11	74	98	160	1.32e-11	57.0
MS.gene54947.t1	MTR_8g062700	48.718	78	32	3	10	81	41	116	1.17e-13	64.7
MS.gene54947.t1	MTR_8g062700	48.718	78	32	3	10	81	41	116	1.17e-13	64.7
MS.gene54947.t1	MTR_5g036670	55.385	65	27	2	10	74	42	104	1.78e-13	63.9
MS.gene54947.t1	MTR_1g104940	47.368	76	36	3	3	78	6	77	2.39e-13	60.5
MS.gene54947.t1	MTR_3g006105	56.250	64	27	1	11	74	8	70	2.73e-13	61.6
MS.gene54947.t1	MTR_3g006065	56.250	64	27	1	11	74	90	152	3.66e-13	61.6
MS.gene54947.t1	MTR_3g006010	54.688	64	28	1	11	74	8	70	5.77e-13	62.4
MS.gene54947.t1	MTR_3g006150	56.250	64	27	1	11	74	90	152	6.39e-13	60.5
MS.gene54947.t1	MTR_7g013410	51.724	58	27	1	17	74	3	59	3.54e-12	58.2
MS.gene54947.t1	MTR_6g008995	47.945	73	36	2	2	74	28	98	7.59e-12	59.3
MS.gene54947.t1	MTR_1g093310	47.826	69	28	3	6	74	4	64	1.56e-11	54.7
MS.gene54947.t1	MTR_1g105960	51.724	58	27	1	17	74	6	62	2.31e-11	55.1
MS.gene54947.t1	MTR_1g091047	51.429	70	29	4	10	78	8	73	3.89e-11	53.5
MS.gene54947.t1	MTR_1g105955	50.909	55	26	1	20	74	103	156	9.91e-11	55.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene54947.t1	AT5G15020	56.923	65	26	2	10	74	51	113	4.95e-14	65.5
MS.gene54947.t1	AT5G15020	56.923	65	26	2	10	74	51	113	5.05e-14	65.5
MS.gene54947.t1	AT3G01320	53.846	65	28	2	10	74	56	118	1.46e-13	64.3
MS.gene54947.t1	AT3G01320	53.846	65	28	2	10	74	56	118	1.57e-13	64.3
MS.gene54947.t1	AT1G24190	50.769	65	30	2	10	74	13	75	5.46e-12	59.7
MS.gene54947.t1	AT1G24190	50.769	65	30	2	10	74	13	75	5.52e-12	59.7
MS.gene54947.t1	AT1G24190	50.769	65	30	2	10	74	13	75	5.57e-12	59.7
MS.gene54947.t1	AT1G59890	51.562	64	29	2	11	74	45	106	1.03e-11	58.9
MS.gene54947.t1	AT1G59890	51.562	64	29	2	11	74	45	106	1.05e-11	58.9
MS.gene54947.t1	AT1G59890	51.562	64	29	2	11	74	45	106	1.05e-11	58.9
MS.gene54947.t1	AT1G59890	51.562	64	29	2	11	74	45	106	1.05e-11	58.9
MS.gene54947.t1	AT1G70060	52.308	65	29	2	10	74	13	75	1.83e-11	58.5
MS.gene54947.t1	AT1G70060	52.308	65	29	2	10	74	13	75	1.83e-11	58.5
MS.gene54947.t1	AT5G15025	50.769	65	30	2	10	74	46	108	3.21e-11	55.1

Find 17 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCACTTGAGTTTCTTCTTA+AGG	0.341291	7.2:+23499920	MS.gene54947:CDS
AAAACTCATAAAAGATTTCA+AGG	0.352278	7.2:+23499995	MS.gene54947:CDS
ACCTTCCTCATCATGCCAAC+TGG	0.443787	7.2:+23500101	MS.gene54947:CDS
GCAGCTCCCTCACTCTTCCT+TGG	0.477319	7.2:-23500043	None:intergenic
GCTGAAAGAATTGATATAAG+AGG	0.511724	7.2:+23500017	MS.gene54947:CDS
AGAGTGAGGGAGCTGCTTCA+AGG	0.529263	7.2:+23500050	MS.gene54947:CDS
CATCATGGCCATTGTTTCGA+GGG	0.532022	7.2:-23499896	None:intergenic
GAGCTGCTTCAAGGACATCC+AGG	0.532286	7.2:+23500059	MS.gene54947:CDS
TCATCATGGCCATTGTTTCG+AGG	0.533555	7.2:-23499897	None:intergenic
GAAACTCAAGTGCATCATCA+TGG	0.574862	7.2:-23499911	None:intergenic
ATGAACATCCCTCGAAACAA+TGG	0.578173	7.2:+23499888	MS.gene54947:CDS
ACGAACCAGTTGGCATGATG+AGG	0.598544	7.2:-23500106	None:intergenic
ATTGATATAAGAGGTGTCCA+AGG	0.601739	7.2:+23500026	MS.gene54947:CDS
AGGTGTCCAAGGAAGAGTGA+GGG	0.605251	7.2:+23500037	MS.gene54947:CDS
TTGAATCTGAATATTAAACC+TGG	0.621609	7.2:-23500077	None:intergenic
ACCAGTTGGCATGATGAGGA+AGG	0.622788	7.2:-23500102	None:intergenic
GAGGTGTCCAAGGAAGAGTG+AGG	0.685556	7.2:+23500036	MS.gene54947:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAACTCATAAAAGATTTCA+AGG	+	chr7.2:23499995-23500014	MS.gene54947:CDS	20.0%
!	AACAGATAAAGCAAAAGTAA+AGG	-	chr7.2:23500188-23500207	None:intergenic	25.0%
!	ATAGATCGTAGAATTATTGA+AGG	-	chr7.2:23500221-23500240	None:intergenic	25.0%
!	TAGATCGTAGAATTATTGAA+GGG	-	chr7.2:23500220-23500239	None:intergenic	25.0%
!	TTGAATCTGAATATTAAACC+TGG	-	chr7.2:23500080-23500099	None:intergenic	25.0%
	GCTGAAAGAATTGATATAAG+AGG	+	chr7.2:23500017-23500036	MS.gene54947:CDS	30.0%
	TCAAGATATGTGAAGTGCAA+TGG	-	chr7.2:23500143-23500162	None:intergenic	35.0%
	TGAAGTGCAATGGAAAAAGT+AGG	-	chr7.2:23500133-23500152	None:intergenic	35.0%
	TGCACTTGAGTTTCTTCTTA+AGG	+	chr7.2:23499920-23499939	MS.gene54947:CDS	35.0%
!!	ATTGATATAAGAGGTGTCCA+AGG	+	chr7.2:23500026-23500045	MS.gene54947:CDS	35.0%
	AAAAGTAGGAACGAACCAGT+TGG	-	chr7.2:23500119-23500138	None:intergenic	40.0%
	ATGAACATCCCTCGAAACAA+TGG	+	chr7.2:23499888-23499907	MS.gene54947:CDS	40.0%
	GAAACTCAAGTGCATCATCA+TGG	-	chr7.2:23499914-23499933	None:intergenic	40.0%
	CATCATGGCCATTGTTTCGA+GGG	-	chr7.2:23499899-23499918	None:intergenic	45.0%
	TCATCATGGCCATTGTTTCG+AGG	-	chr7.2:23499900-23499919	None:intergenic	45.0%
	ACCAGTTGGCATGATGAGGA+AGG	-	chr7.2:23500105-23500124	None:intergenic	50.0%
	ACCTTCCTCATCATGCCAAC+TGG	+	chr7.2:23500101-23500120	MS.gene54947:CDS	50.0%
	ACGAACCAGTTGGCATGATG+AGG	-	chr7.2:23500109-23500128	None:intergenic	50.0%
	AGGTGTCCAAGGAAGAGTGA+GGG	+	chr7.2:23500037-23500056	MS.gene54947:CDS	50.0%
	AGAGTGAGGGAGCTGCTTCA+AGG	+	chr7.2:23500050-23500069	MS.gene54947:CDS	55.0%
	GAGCTGCTTCAAGGACATCC+AGG	+	chr7.2:23500059-23500078	MS.gene54947:CDS	55.0%
!	GAGGTGTCCAAGGAAGAGTG+AGG	+	chr7.2:23500036-23500055	MS.gene54947:CDS	55.0%
	GCAGCTCCCTCACTCTTCCT+TGG	-	chr7.2:23500046-23500065	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	23499888	23500275	23499888	ID=MS.gene54947
chr7.2	mRNA	23499888	23500275	23499888	ID=MS.gene54947.t1;Parent=MS.gene54947
chr7.2	exon	23499888	23500122	23499888	ID=MS.gene54947.t1.exon1;Parent=MS.gene54947.t1
chr7.2	CDS	23499888	23500122	23499888	ID=cds.MS.gene54947.t1;Parent=MS.gene54947.t1
chr7.2	exon	23500265	23500275	23500265	ID=MS.gene54947.t1.exon2;Parent=MS.gene54947.t1
chr7.2	CDS	23500265	23500275	23500265	ID=cds.MS.gene54947.t1;Parent=MS.gene54947.t1

Gene Sequence

>MS.gene54947

ATGAACATCCCTCGAAACAATGGCCATGATGATGCACTTGAGTTTCTTCTTAAGGAAGTCAAACTTGCATGTAAGTATGATAACAAGTTAAAGTATGATGATTTTTTAAAACTCATAAAAGATTTCAAGGCTGAAAGAATTGATATAAGAGGTGTCCAAGGAAGAGTGAGGGAGCTGCTTCAAGGACATCCAGGTTTAATATTCAGATTCAACACCTTCCTCATCATGCCAACTGGTTCGTTCCTACTTTTTCCATTGCACTTCACATATCTTGATACTATATATGTTTACTATATGCCTTTACTTTTGCTTTATCTGTTTGTATTCAACCCTTCAATAATTCTACGATCTATTGTTTTTCTTCGTATTATTGTCAGATAAACAATAA

Protein sequence

>MS.gene54947.t1

MNIPRNNGHDDALEFLLKEVKLACKYDNKLKYDDFLKLIKDFKAERIDIRGVQGRVRELLQGHPGLIFRFNTFLIMPTDKQ