AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54980.t1 MTR_7g083360 71.154 156 44 1 1 156 1 155 2.73e-80 234
MS.gene54980.t1 MTR_7g083330 70.779 154 45 0 3 156 11 164 1.76e-77 228
MS.gene54980.t1 MTR_7g083370 76.087 138 33 0 1 138 1 138 3.30e-77 226
MS.gene54980.t1 MTR_2157s0010 31.469 143 91 4 3 140 1 141 3.98e-20 84.0
MS.gene54980.t1 MTR_7g085220 31.884 138 81 4 10 136 8 143 2.68e-19 82.8
MS.gene54980.t1 MTR_4g081870 29.412 153 100 5 4 153 3 150 4.30e-19 81.6
MS.gene54980.t1 MTR_2g064090 32.716 162 90 7 6 155 2 156 6.20e-19 82.0
MS.gene54980.t1 MTR_3g088110 33.588 131 80 4 6 133 3 129 6.48e-19 81.3
MS.gene54980.t1 MTR_5g041940 30.201 149 96 5 8 153 1 144 8.02e-19 80.5
MS.gene54980.t1 MTR_7g005280 32.394 142 87 5 5 142 10 146 9.50e-19 80.1
MS.gene54980.t1 MTR_4g089135 33.333 144 88 5 10 147 15 156 1.51e-18 80.9
MS.gene54980.t1 MTR_8g094580 33.333 129 79 4 8 133 5 129 4.46e-18 79.0
MS.gene54980.t1 MTR_2g093810 30.714 140 91 4 4 139 8 145 1.15e-17 78.2
MS.gene54980.t1 MTR_7g100990 32.639 144 87 5 6 141 7 148 1.20e-17 78.2
MS.gene54980.t1 MTR_5g090970 35.338 133 76 6 7 133 16 144 2.26e-17 77.4
MS.gene54980.t1 MTR_6g011860 31.333 150 95 5 1 145 7 153 2.57e-17 77.8
MS.gene54980.t1 MTR_5g040420 32.847 137 85 5 7 139 12 145 2.64e-17 77.8
MS.gene54980.t1 MTR_5g090970 35.338 133 75 6 7 133 16 143 5.14e-17 76.3
MS.gene54980.t1 MTR_6g477900 34.646 127 79 3 10 133 6 131 5.76e-17 75.1
MS.gene54980.t1 MTR_2g080010 33.594 128 79 4 10 133 15 140 6.24e-17 76.6
MS.gene54980.t1 MTR_3g070030 31.293 147 92 5 3 143 12 155 8.32e-17 76.3
MS.gene54980.t1 MTR_3g096920 32.836 134 77 5 6 133 3 129 1.54e-16 74.7
MS.gene54980.t1 MTR_2g068920 32.867 143 88 5 3 139 15 155 1.74e-16 74.7
MS.gene54980.t1 MTR_8g059170 29.221 154 93 6 6 153 10 153 1.75e-16 75.1
MS.gene54980.t1 MTR_2g068880 32.394 142 88 5 4 139 17 156 1.76e-16 75.1
MS.gene54980.t1 MTR_8g467490 32.847 137 85 5 7 139 12 145 1.89e-16 75.5
MS.gene54980.t1 MTR_3g096920 32.836 134 77 5 6 133 3 129 2.09e-16 74.7
MS.gene54980.t1 MTR_6g012670 31.884 138 88 4 4 138 14 148 2.25e-16 74.7
MS.gene54980.t1 MTR_6g032770 29.932 147 94 5 4 143 12 156 2.77e-16 74.7
MS.gene54980.t1 MTR_8g099750 29.496 139 90 4 3 137 7 141 3.16e-16 73.2
MS.gene54980.t1 MTR_5g076850 33.588 131 83 3 6 133 2 131 3.39e-16 75.1
MS.gene54980.t1 MTR_3g064580 32.824 131 84 3 6 133 2 131 3.72e-16 74.7
MS.gene54980.t1 MTR_1g008740 32.117 137 84 5 7 137 10 143 7.79e-16 73.6
MS.gene54980.t1 MTR_1g008740 32.117 137 84 5 7 137 24 157 8.69e-16 73.6
MS.gene54980.t1 MTR_2g078700 31.250 128 82 5 10 133 23 148 9.10e-16 73.6
MS.gene54980.t1 MTR_6g084430 31.496 127 81 5 10 133 5 128 1.41e-15 72.4
MS.gene54980.t1 MTR_2g079990 30.147 136 89 4 6 138 12 144 1.98e-15 72.4
MS.gene54980.t1 MTR_2g079990 30.147 136 89 4 6 138 12 144 2.10e-15 72.4
MS.gene54980.t1 MTR_3g116070 30.714 140 88 5 7 141 20 155 3.43e-15 71.6
MS.gene54980.t1 MTR_1g096430 29.677 155 100 5 7 155 4 155 3.60e-15 71.6
MS.gene54980.t1 MTR_3g435150 28.462 130 87 5 7 133 13 139 5.25e-15 70.9
MS.gene54980.t1 MTR_3g109340 31.111 135 87 4 7 137 19 151 5.52e-15 71.2
MS.gene54980.t1 MTR_3g109340 31.111 135 87 4 7 137 19 151 5.61e-15 71.2
MS.gene54980.t1 MTR_3g093050 33.071 127 81 3 10 133 22 147 6.82e-15 71.2
MS.gene54980.t1 MTR_1g090723 32.215 149 93 7 13 155 11 157 6.83e-15 71.2
MS.gene54980.t1 MTR_1g090723 32.215 149 93 7 13 155 11 157 7.29e-15 71.2
MS.gene54980.t1 MTR_3g093050 33.071 127 81 3 10 133 22 147 9.66e-15 70.9
MS.gene54980.t1 MTR_5g021710 30.519 154 100 5 7 155 5 156 9.92e-15 70.5
MS.gene54980.t1 MTR_7g085260 32.576 132 81 5 8 133 15 144 1.11e-14 70.5
MS.gene54980.t1 MTR_1g090723 33.333 129 79 6 13 136 11 137 1.24e-14 70.5
MS.gene54980.t1 MTR_4g036030 28.667 150 102 4 10 155 7 155 1.55e-14 69.7
MS.gene54980.t1 MTR_1g090720 32.031 128 81 4 10 133 42 167 2.58e-14 69.3
MS.gene54980.t1 MTR_2g086880 31.884 138 87 7 1 135 11 144 2.78e-14 69.3
MS.gene54980.t1 MTR_2g086880 31.884 138 87 7 1 135 11 144 3.07e-14 69.3
MS.gene54980.t1 MTR_5g069030 33.858 127 80 3 10 133 25 150 3.19e-14 69.3
MS.gene54980.t1 MTR_1g069805 31.333 150 94 6 1 145 1 146 3.73e-14 68.2
MS.gene54980.t1 MTR_3g098810 29.078 141 90 4 3 137 7 143 5.26e-14 66.2
MS.gene54980.t1 MTR_4g108760 28.462 130 87 5 7 133 14 140 5.32e-14 68.2
MS.gene54980.t1 MTR_1g097300 29.054 148 99 4 13 155 22 168 5.92e-14 68.6
MS.gene54980.t1 MTR_3g093040 31.008 129 85 3 8 133 106 233 1.39e-13 67.0
MS.gene54980.t1 MTR_3g098810 29.078 141 90 4 3 137 7 143 1.51e-13 66.2
MS.gene54980.t1 MTR_8g023880 28.671 143 80 4 10 133 7 146 2.05e-13 66.2
MS.gene54980.t1 MTR_8g076110 28.105 153 105 4 7 155 3 154 2.44e-13 66.2
MS.gene54980.t1 MTR_5g014300 27.564 156 100 4 10 156 6 157 2.66e-13 66.2
MS.gene54980.t1 MTR_0036s0150 30.882 136 73 6 13 136 8 134 3.86e-13 66.2
MS.gene54980.t1 MTR_5g012080 28.906 128 87 3 10 133 7 134 4.71e-13 65.5
MS.gene54980.t1 MTR_7g097090 31.126 151 93 6 1 145 1 146 4.93e-13 65.1
MS.gene54980.t1 MTR_4g101680 27.815 151 102 5 10 155 7 155 6.41e-13 65.5
MS.gene54980.t1 MTR_2g064470 29.787 141 81 6 3 135 4 134 8.62e-13 64.7
MS.gene54980.t1 MTR_2g062730 27.044 159 112 3 1 155 1 159 1.02e-12 64.7
MS.gene54980.t1 MTR_8g023900 28.966 145 97 4 9 148 11 154 1.27e-12 64.7
MS.gene54980.t1 MTR_7g011120 29.545 132 87 4 5 133 3 131 2.10e-12 63.5
MS.gene54980.t1 MTR_4g134460 26.316 152 106 4 7 155 52 200 2.98e-12 63.5
MS.gene54980.t1 MTR_7g011130 28.358 134 90 4 3 133 12 142 1.19e-11 61.6
MS.gene54980.t1 MTR_4g098630 25.490 153 107 4 10 155 6 158 1.41e-11 61.2
MS.gene54980.t1 MTR_8g023840 31.008 129 83 5 10 133 11 138 4.37e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54980.t1 AT5G13180 37.037 135 76 5 3 133 7 136 1.38e-20 85.1
MS.gene54980.t1 AT2G33480 33.582 134 80 4 8 138 13 140 7.10e-20 83.6
MS.gene54980.t1 AT2G33480 33.582 134 80 4 8 138 13 140 7.47e-20 83.6
MS.gene54980.t1 AT2G43000 35.897 156 88 8 7 155 16 166 1.69e-19 82.8
MS.gene54980.t1 AT3G03200 33.333 162 89 5 6 155 2 156 8.16e-19 82.4
MS.gene54980.t1 AT1G61110 34.646 127 78 4 10 133 16 140 1.31e-18 80.9
MS.gene54980.t1 AT1G77450 33.088 136 84 4 1 133 1 132 1.99e-18 79.3
MS.gene54980.t1 AT5G39820 32.168 143 88 6 7 143 18 157 2.43e-18 80.5
MS.gene54980.t1 AT1G76420 31.788 151 95 5 10 156 22 168 4.31e-18 79.7
MS.gene54980.t1 AT5G63790 32.824 131 81 4 6 133 46 172 4.47e-18 79.3
MS.gene54980.t1 AT5G63790 32.824 131 81 4 6 133 56 182 5.27e-18 79.3
MS.gene54980.t1 AT3G10500 33.846 130 82 4 10 136 9 137 6.62e-18 79.7
MS.gene54980.t1 AT1G65910 32.824 131 84 3 6 133 2 131 8.86e-18 79.7
MS.gene54980.t1 AT5G08790 32.061 131 82 4 6 133 3 129 1.04e-17 77.8
MS.gene54980.t1 AT1G69490 32.090 134 83 5 4 133 3 132 1.85e-17 77.0
MS.gene54980.t1 AT5G18270 33.071 127 80 4 10 133 21 145 2.41e-17 77.4
MS.gene54980.t1 AT3G17730 34.615 130 83 2 6 133 2 131 2.71e-17 76.3
MS.gene54980.t1 AT1G26870 34.091 132 78 6 7 133 14 141 4.41e-17 77.4
MS.gene54980.t1 AT5G17260 31.481 162 92 5 6 155 2 156 4.96e-17 77.4
MS.gene54980.t1 AT3G04070 28.767 146 95 4 1 139 1 144 5.31e-17 76.6
MS.gene54980.t1 AT3G04070 29.927 137 87 4 10 139 10 144 7.66e-17 76.3
MS.gene54980.t1 AT3G15170 31.298 131 84 5 7 133 17 145 8.93e-17 75.9
MS.gene54980.t1 AT5G04410 30.709 127 84 3 10 133 9 134 1.07e-16 76.3
MS.gene54980.t1 AT2G24430 29.221 154 102 5 7 155 13 164 1.24e-16 75.5
MS.gene54980.t1 AT2G24430 29.221 154 102 5 7 155 13 164 1.24e-16 75.5
MS.gene54980.t1 AT5G18270 32.283 127 81 4 10 133 21 145 1.56e-16 75.5
MS.gene54980.t1 AT5G53950 32.812 128 80 5 10 133 17 142 1.83e-16 75.5
MS.gene54980.t1 AT3G12910 35.338 133 75 5 8 133 20 148 3.04e-16 74.3
MS.gene54980.t1 AT3G15500 32.836 134 84 4 6 136 10 140 3.48e-16 74.3
MS.gene54980.t1 AT3G04060 30.709 127 83 4 10 133 20 144 3.80e-16 74.3
MS.gene54980.t1 AT1G01720 32.576 132 81 5 6 133 2 129 4.38e-16 73.6
MS.gene54980.t1 AT3G15510 32.283 127 80 4 14 136 21 145 5.49e-16 73.9
MS.gene54980.t1 AT1G33060 33.858 127 80 4 10 133 24 149 6.10e-16 74.3
MS.gene54980.t1 AT1G33060 33.858 127 80 4 10 133 24 149 6.58e-16 74.3
MS.gene54980.t1 AT1G52890 31.034 145 90 5 6 147 10 147 9.42e-16 73.2
MS.gene54980.t1 AT5G07680 28.846 156 104 5 5 156 12 164 9.45e-16 73.2
MS.gene54980.t1 AT3G10490 30.935 139 90 4 2 136 19 155 9.98e-16 72.0
MS.gene54980.t1 AT5G46590 26.490 151 108 2 9 156 5 155 1.28e-15 72.4
MS.gene54980.t1 AT5G07680 29.801 151 99 5 10 156 3 150 1.40e-15 72.4
MS.gene54980.t1 AT3G29035 30.065 153 95 6 10 154 24 172 1.52e-15 72.4
MS.gene54980.t1 AT1G54330 32.075 159 91 5 6 155 2 152 1.58e-15 72.4
MS.gene54980.t1 AT4G27410 30.597 134 87 4 6 136 10 140 1.89e-15 72.0
MS.gene54980.t1 AT5G39610 32.283 127 80 5 10 133 20 143 1.97e-15 71.6
MS.gene54980.t1 AT2G27300 32.278 158 95 8 1 155 7 155 3.00e-15 71.6
MS.gene54980.t1 AT5G62380 29.801 151 99 5 10 155 7 155 3.44e-15 71.6
MS.gene54980.t1 AT5G62380 29.801 151 99 5 10 155 7 155 3.44e-15 71.6
MS.gene54980.t1 AT4G36160 29.375 160 106 5 1 155 5 162 3.62e-15 71.6
MS.gene54980.t1 AT4G35580 34.646 127 79 4 10 133 9 134 3.88e-15 72.0
MS.gene54980.t1 AT4G35580 34.646 127 79 4 10 133 9 134 4.19e-15 72.0
MS.gene54980.t1 AT4G35580 34.646 127 79 4 10 133 9 134 4.24e-15 72.0
MS.gene54980.t1 AT4G36160 29.375 160 106 5 1 155 1 158 4.48e-15 71.6
MS.gene54980.t1 AT4G36160 29.375 160 106 5 1 155 1 158 4.48e-15 71.6
MS.gene54980.t1 AT3G18400 30.534 131 85 5 10 137 5 132 5.33e-15 70.9
MS.gene54980.t1 AT5G61430 30.769 130 84 5 7 133 13 139 7.15e-15 70.9
MS.gene54980.t1 AT3G10490 30.935 139 90 4 2 136 19 155 9.56e-15 70.9
MS.gene54980.t1 AT3G10480 30.216 139 91 4 2 136 19 155 1.40e-14 70.1
MS.gene54980.t1 AT3G10480 30.216 139 91 4 2 136 19 155 1.50e-14 70.1
MS.gene54980.t1 AT3G44290 32.283 127 81 4 13 136 17 141 1.70e-14 68.6
MS.gene54980.t1 AT4G17980 26.797 153 107 4 9 156 5 157 1.89e-14 68.9
MS.gene54980.t1 AT1G71930 28.667 150 102 4 10 155 9 157 2.04e-14 69.3
MS.gene54980.t1 AT1G71930 28.667 150 102 4 10 155 9 157 2.04e-14 69.3
MS.gene54980.t1 AT4G17980 26.797 153 107 4 9 156 5 157 3.24e-14 68.6
MS.gene54980.t1 AT5G46590 28.906 128 88 2 9 133 5 132 3.77e-14 68.2
MS.gene54980.t1 AT1G34180 32.308 130 72 4 13 133 19 141 4.09e-14 68.9
MS.gene54980.t1 AT3G44290 32.283 127 81 4 13 136 17 141 5.75e-14 68.2
MS.gene54980.t1 AT3G12977 28.472 144 95 5 2 141 10 149 6.85e-14 67.4
MS.gene54980.t1 AT1G56010 29.655 145 94 5 2 142 11 151 8.51e-14 67.8
MS.gene54980.t1 AT5G22290 30.657 137 89 5 2 135 14 147 1.13e-13 67.4
MS.gene54980.t1 AT1G79580 27.941 136 94 3 2 133 9 144 1.30e-13 67.4
MS.gene54980.t1 AT1G79580 27.941 136 94 3 2 133 9 144 1.30e-13 67.4
MS.gene54980.t1 AT1G79580 27.941 136 94 3 2 133 9 144 1.30e-13 67.4
MS.gene54980.t1 AT1G79580 27.941 136 94 3 2 133 9 144 1.30e-13 67.4
MS.gene54980.t1 AT1G79580 27.941 136 94 3 2 133 9 144 1.30e-13 67.4
MS.gene54980.t1 AT2G18060 29.801 151 99 5 10 155 9 157 1.37e-13 67.4
MS.gene54980.t1 AT2G18060 29.801 151 99 5 10 155 9 157 1.37e-13 67.4
MS.gene54980.t1 AT5G66300 29.801 151 100 5 10 155 12 161 1.68e-13 66.6
MS.gene54980.t1 AT1G34190 30.769 130 74 4 13 133 19 141 1.87e-13 67.0
MS.gene54980.t1 AT5G50820 28.834 163 101 4 2 156 9 164 2.91e-13 64.7
MS.gene54980.t1 AT1G34180 31.818 132 74 4 13 135 19 143 3.64e-13 66.2
MS.gene54980.t1 AT1G12260 27.333 150 104 4 10 155 7 155 4.20e-13 65.9
MS.gene54980.t1 AT5G24590 31.496 127 84 2 10 133 13 139 5.16e-13 65.9
MS.gene54980.t1 AT1G12260 27.333 150 104 4 10 155 7 155 7.28e-13 65.1
MS.gene54980.t1 AT5G14000 28.058 139 88 6 3 135 8 140 8.98e-13 62.4
MS.gene54980.t1 AT5G04395 29.605 152 89 6 1 135 19 169 1.79e-12 62.4
MS.gene54980.t1 AT2G02450 25.828 151 106 4 8 155 50 197 1.94e-12 63.9
MS.gene54980.t1 AT2G02450 25.828 151 106 4 8 155 50 197 2.48e-12 63.9
MS.gene54980.t1 AT5G09330 33.858 127 80 4 10 133 6 131 2.74e-12 63.5
MS.gene54980.t1 AT5G09330 33.858 127 80 4 10 133 6 131 2.74e-12 63.5
MS.gene54980.t1 AT5G09330 33.858 127 80 4 10 133 6 131 2.74e-12 63.5
MS.gene54980.t1 AT5G09330 33.858 127 80 4 10 133 6 131 2.74e-12 63.5
MS.gene54980.t1 AT5G09330 33.858 127 80 4 10 133 6 131 2.74e-12 63.5
MS.gene54980.t1 AT5G14000 28.058 139 88 6 3 135 8 140 4.10e-12 61.6
MS.gene54980.t1 AT4G28530 28.758 153 87 4 1 133 1 151 5.94e-12 62.4
MS.gene54980.t1 AT3G49530 29.134 127 87 2 10 133 13 139 1.77e-11 61.2
MS.gene54980.t1 AT3G44350 29.630 135 89 3 10 138 5 139 2.76e-11 58.5
MS.gene54980.t1 AT1G32870 29.412 153 97 4 10 156 10 157 3.12e-11 60.8
MS.gene54980.t1 AT1G32870 29.412 153 97 4 10 156 46 193 3.31e-11 60.5
MS.gene54980.t1 AT1G32870 29.412 153 97 4 10 156 10 157 3.49e-11 60.5
MS.gene54980.t1 AT1G32510 28.358 134 87 3 9 133 5 138 7.26e-11 59.3
MS.gene54980.t1 AT1G52880 29.365 126 81 4 14 133 21 144 8.73e-11 59.3

Find 37 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGAACACATTTGTTTAC+TGG 0.271032 7.2:+23894039 MS.gene54980:CDS
AGTACTATTTCTTTGATATC+AGG 0.279804 7.2:+23893910 MS.gene54980:CDS
AGGTGCCTATCGGATATTTA+AGG 0.283056 7.2:+23894354 MS.gene54980:CDS
CTTTCACACAATCTATTGAT+TGG 0.327245 7.2:+23894014 MS.gene54980:CDS
GAACCGTAGATTCGAGAATA+TGG 0.360249 7.2:+23893932 MS.gene54980:CDS
TTTCACACAATCTATTGATT+GGG 0.415597 7.2:+23894015 MS.gene54980:CDS
ATTGTATTCATTACCCTAGC+TGG 0.431951 7.2:-23894137 None:intergenic
ACACATTTGTTTACTGGATA+GGG 0.438722 7.2:+23894045 MS.gene54980:CDS
GGATCAAACTTGTATCCAAC+AGG 0.462546 7.2:-23893380 None:intergenic
GCTGAGCTTCTTCTACCTGT+TGG 0.469612 7.2:+23893365 MS.gene54980:CDS
AACACATTTGTTTACTGGAT+AGG 0.480417 7.2:+23894044 MS.gene54980:CDS
AAACCTCTTGGCAGCCTCCA+TGG 0.486538 7.2:-23893500 None:intergenic
GAAGAGCTGGAGATGGTGAA+TGG 0.497231 7.2:+23893961 MS.gene54980:CDS
CATCCATATTCTCGAATCTA+CGG 0.497709 7.2:-23893935 None:intergenic
TCGAATTCAAACAAGTGAGT+TGG 0.514689 7.2:-23893461 None:intergenic
AATATGGATGCAAGAAGAGC+TGG 0.523452 7.2:+23893948 MS.gene54980:CDS
ATCGGATATTTAAGGTATGA+AGG 0.524239 7.2:+23894362 MS.gene54980:CDS
CTTGTTGAGTACTACCTGAC+GGG 0.535841 7.2:+23893416 MS.gene54980:CDS
TTTGTTTACTGGATAGGGCA+AGG 0.546149 7.2:+23894050 MS.gene54980:CDS
GTGAATGGAGAATAGTTGAA+TGG 0.546179 7.2:+23893976 MS.gene54980:CDS
GTACAATTCATCATCCAGCT+AGG 0.552549 7.2:+23894123 MS.gene54980:CDS
TCAACAAGCTCGTGATCAGT+TGG 0.555838 7.2:-23893401 None:intergenic
TCAAACAAGTGAGTTGGAAG+AGG 0.558737 7.2:-23893455 None:intergenic
GAGAATAGTTGAATGGAATG+AGG 0.563671 7.2:+23893983 MS.gene54980:CDS
ATGTGTTTCAAACTGAGCCA+TGG 0.569635 7.2:+23893483 MS.gene54980:CDS
TACAATTCATCATCCAGCTA+GGG 0.576583 7.2:+23894124 MS.gene54980:CDS
AGACAGCTGTAGGTGCCTAT+CGG 0.582547 7.2:+23894344 MS.gene54980:CDS
GCTTGTTGAGTACTACCTGA+CGG 0.620029 7.2:+23893415 MS.gene54980:CDS
GATGCAAGAAGAGCTGGAGA+TGG 0.639988 7.2:+23893954 MS.gene54980:CDS
TCATACCTTAAATATCCGAT+AGG 0.640099 7.2:-23894359 None:intergenic
TCAATGTAGGAGACAGCTGT+AGG 0.645111 7.2:+23894334 MS.gene54980:intron
ACAAGTGAGTTGGAAGAGGT+AGG 0.647632 7.2:-23893451 None:intergenic
ATCAAGCTCTGATGACACAA+TGG 0.656016 7.2:+23894075 MS.gene54980:CDS
GAGCCATGGAGGCTGCCAAG+AGG 0.670912 7.2:+23893497 MS.gene54980:CDS
TTGTTGAGTACTACCTGACG+GGG 0.672691 7.2:+23893417 MS.gene54980:CDS
AAGCTCTGATGACACAATGG+TGG 0.675163 7.2:+23894078 MS.gene54980:CDS
TGTTTCAAACTGAGCCATGG+AGG 0.716472 7.2:+23893486 MS.gene54980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACAATTTGATTTATTCATTT+TGG + chr7.2:23893815-23893834 MS.gene54980:intron 15.0%
!!! CAATTTGATTTATTCATTTT+GGG + chr7.2:23893816-23893835 MS.gene54980:intron 15.0%
!!! TATCTTGTTTATTTATAATC+AGG + chr7.2:23894278-23894297 MS.gene54980:intron 15.0%
!!! TTTTTTTAAGTAATGTGTTT+TGG + chr7.2:23894199-23894218 MS.gene54980:intron 15.0%
!! AACATCATATAAACATTTCA+TGG - chr7.2:23893777-23893796 None:intergenic 20.0%
!! ACATCATATAAACATTTCAT+GGG - chr7.2:23893776-23893795 None:intergenic 20.0%
!! CCATCAAAAGATTAATTAAA+TGG - chr7.2:23893670-23893689 None:intergenic 20.0%
!!! CCATTTAATTAATCTTTTGA+TGG + chr7.2:23893667-23893686 MS.gene54980:intron 20.0%
!!! TTTATATGATGTTTTCATGT+GGG + chr7.2:23893784-23893803 MS.gene54980:intron 20.0%
!!! TTTTTTTTTTTGCAGATGAT+AGG + chr7.2:23893868-23893887 MS.gene54980:intron 20.0%
! TTTCTATTGATAAGACACAA+TGG - chr7.2:23893594-23893613 None:intergenic 25.0%
!! AGTACTATTTCTTTGATATC+AGG + chr7.2:23893910-23893929 MS.gene54980:CDS 25.0%
!! GTTTATATGATGTTTTCATG+TGG + chr7.2:23893783-23893802 MS.gene54980:intron 25.0%
!! TTTCACACAATCTATTGATT+GGG + chr7.2:23894015-23894034 MS.gene54980:CDS 25.0%
!! TTTTGAACTCAAATCAATGT+AGG + chr7.2:23894321-23894340 MS.gene54980:intron 25.0%
!!! AATCCTCAAAAGTTTTTTGA+TGG - chr7.2:23894177-23894196 None:intergenic 25.0%
!!! TGCTAGTTTTTATGTAAACT+TGG + chr7.2:23894247-23894266 MS.gene54980:intron 25.0%
AACACATTTGTTTACTGGAT+AGG + chr7.2:23894044-23894063 MS.gene54980:CDS 30.0%
ACACATTTGTTTACTGGATA+GGG + chr7.2:23894045-23894064 MS.gene54980:CDS 30.0%
CTTTCACACAATCTATTGAT+TGG + chr7.2:23894014-23894033 MS.gene54980:CDS 30.0%
GAAAGAACACATTTGTTTAC+TGG + chr7.2:23894039-23894058 MS.gene54980:CDS 30.0%
TCATACCTTAAATATCCGAT+AGG - chr7.2:23894362-23894381 None:intergenic 30.0%
! TCACCATCAAAAAACTTTTG+AGG + chr7.2:23894171-23894190 MS.gene54980:intron 30.0%
!! AGTAATGTGTTTTGGACTTA+TGG + chr7.2:23894207-23894226 MS.gene54980:intron 30.0%
!!! AAGTTTTTTGATGGTGATAG+TGG - chr7.2:23894168-23894187 None:intergenic 30.0%
ATTGTATTCATTACCCTAGC+TGG - chr7.2:23894140-23894159 None:intergenic 35.0%
CATCCATATTCTCGAATCTA+CGG - chr7.2:23893938-23893957 None:intergenic 35.0%
CTGAAATTTCACAAACCTCT+TGG - chr7.2:23893515-23893534 None:intergenic 35.0%
GAGAATAGTTGAATGGAATG+AGG + chr7.2:23893983-23894002 MS.gene54980:CDS 35.0%
GTGAATGGAGAATAGTTGAA+TGG + chr7.2:23893976-23893995 MS.gene54980:CDS 35.0%
TACAATTCATCATCCAGCTA+GGG + chr7.2:23894124-23894143 MS.gene54980:CDS 35.0%
TCGAATTCAAACAAGTGAGT+TGG - chr7.2:23893464-23893483 None:intergenic 35.0%
! TGCAGATGATAGGTTTTCTT+TGG + chr7.2:23893878-23893897 MS.gene54980:intron 35.0%
!!! ATAGGTTTTCTTTGGAGCAT+AGG + chr7.2:23893886-23893905 MS.gene54980:CDS 35.0%
AATATGGATGCAAGAAGAGC+TGG + chr7.2:23893948-23893967 MS.gene54980:CDS 40.0%
AGGTGCCTATCGGATATTTA+AGG + chr7.2:23894354-23894373 MS.gene54980:CDS 40.0%
ATGTGTTTCAAACTGAGCCA+TGG + chr7.2:23893483-23893502 MS.gene54980:CDS 40.0%
GAACCGTAGATTCGAGAATA+TGG + chr7.2:23893932-23893951 MS.gene54980:CDS 40.0%
GGATCAAACTTGTATCCAAC+AGG - chr7.2:23893383-23893402 None:intergenic 40.0%
GTACAATTCATCATCCAGCT+AGG + chr7.2:23894123-23894142 MS.gene54980:CDS 40.0%
TCTTAGACATGCAGTTGGAA+GGG + chr7.2:23893720-23893739 MS.gene54980:intron 40.0%
TTTGTTTACTGGATAGGGCA+AGG + chr7.2:23894050-23894069 MS.gene54980:CDS 40.0%
! ATCAAGCTCTGATGACACAA+TGG + chr7.2:23894075-23894094 MS.gene54980:CDS 40.0%
! TCAAACAAGTGAGTTGGAAG+AGG - chr7.2:23893458-23893477 None:intergenic 40.0%
CTTGTTGAGTACTACCTGAC+GGG + chr7.2:23893416-23893435 MS.gene54980:CDS 45.0%
GTCTTAGACATGCAGTTGGA+AGG + chr7.2:23893719-23893738 MS.gene54980:intron 45.0%
GTGTGTCTTAGACATGCAGT+TGG + chr7.2:23893715-23893734 MS.gene54980:intron 45.0%
TCAACAAGCTCGTGATCAGT+TGG - chr7.2:23893404-23893423 None:intergenic 45.0%
TCAATGTAGGAGACAGCTGT+AGG + chr7.2:23894334-23894353 MS.gene54980:intron 45.0%
TGTTTCAAACTGAGCCATGG+AGG + chr7.2:23893486-23893505 MS.gene54980:CDS 45.0%
TTGTTGAGTACTACCTGACG+GGG + chr7.2:23893417-23893436 MS.gene54980:CDS 45.0%
! AAGCTCTGATGACACAATGG+TGG + chr7.2:23894078-23894097 MS.gene54980:CDS 45.0%
! ACAAGTGAGTTGGAAGAGGT+AGG - chr7.2:23893454-23893473 None:intergenic 45.0%
! GCTTGTTGAGTACTACCTGA+CGG + chr7.2:23893415-23893434 MS.gene54980:CDS 45.0%
!!! ACAAAAATTTAAAATTTAAA+TGG - chr7.2:23893634-23893653 None:intergenic 5.0%
AGACAGCTGTAGGTGCCTAT+CGG + chr7.2:23894344-23894363 MS.gene54980:CDS 50.0%
GAAGAGCTGGAGATGGTGAA+TGG + chr7.2:23893961-23893980 MS.gene54980:CDS 50.0%
GATGCAAGAAGAGCTGGAGA+TGG + chr7.2:23893954-23893973 MS.gene54980:CDS 50.0%
GCTGAGCTTCTTCTACCTGT+TGG + chr7.2:23893365-23893384 MS.gene54980:CDS 50.0%
TAGACATGCAGTTGGAAGGG+AGG + chr7.2:23893723-23893742 MS.gene54980:intron 50.0%
! GGTTGAGAATTTTCCCCGTC+AGG - chr7.2:23893433-23893452 None:intergenic 50.0%
AAACCTCTTGGCAGCCTCCA+TGG - chr7.2:23893503-23893522 None:intergenic 55.0%
GAGCCATGGAGGCTGCCAAG+AGG + chr7.2:23893497-23893516 MS.gene54980:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 23893350 23894381 23893350 ID=MS.gene54980
chr7.2 mRNA 23893350 23894381 23893350 ID=MS.gene54980.t1;Parent=MS.gene54980
chr7.2 exon 23893350 23893518 23893350 ID=MS.gene54980.t1.exon1;Parent=MS.gene54980.t1
chr7.2 CDS 23893350 23893518 23893350 ID=cds.MS.gene54980.t1;Parent=MS.gene54980.t1
chr7.2 exon 23893883 23894145 23893883 ID=MS.gene54980.t1.exon2;Parent=MS.gene54980.t1
chr7.2 CDS 23893883 23894145 23893883 ID=cds.MS.gene54980.t1;Parent=MS.gene54980.t1
chr7.2 exon 23894343 23894381 23894343 ID=MS.gene54980.t1.exon3;Parent=MS.gene54980.t1
chr7.2 CDS 23894343 23894381 23894343 ID=cds.MS.gene54980.t1;Parent=MS.gene54980.t1
Gene Sequence

>MS.gene54980

ATGGCTGTAGCTAATGCTGAGCTTCTTCTACCTGTTGGATACAAGTTTGATCCAACTGATCACGAGCTTGTTGAGTACTACCTGACGGGGAAAATTCTCAACCTACCTCTTCCAACTCACTTGTTTGAATTCGATGTGTTTCAAACTGAGCCATGGAGGCTGCCAAGAGGTTTGTGAAATTTCAGTTTTTGATAGAATAATAAATGCTCTTTTTTTAATGTTTATATTCTTTTTCTTACGACCATTGTGTCTTATCAATAGAAATCAAATTTTTACAAGAGCCATTTAAATTTTAAATTTTTGTAGTGTTCTTTGAACCATTTAATTAATCTTTTGATGGATTTTCATTAATCTTGAGAATGAACGTGTGTCTTAGACATGCAGTTGGAAGGGAGGAGACTTAAAAAGTCATTTTTTTTATGTCCCATGAAATGTTTATATGATGTTTTCATGTGGGTAATTAACACAATTTGATTTATTCATTTTGGGTCAATTCAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAGATGATAGGTTTTCTTTGGAGCATAGGAAGTACTATTTCTTTGATATCAGGAACCGTAGATTCGAGAATATGGATGCAAGAAGAGCTGGAGATGGTGAATGGAGAATAGTTGAATGGAATGAGGATTTTGCTCTTTCACACAATCTATTGATTGGGAGAAAGAACACATTTGTTTACTGGATAGGGCAAGGAAATCAAGCTCTGATGACACAATGGTGGATGCACGAATTCTTCATCATCAGTACAATTCATCATCCAGCTAGGGTAATGAATACAATATTGACCACTATCACCATCAAAAAACTTTTGAGGATTTTTTTTTTTAAGTAATGTGTTTTGGACTTATGGTATTTTCAGCTAAAAATTGCTAGTTTTTATGTAAACTTGGTTCGTTTTTATCTTGTTTATTTATAATCAGGAACTATATATAGTCACTGAATTTTGAACTCAAATCAATGTAGGAGACAGCTGTAGGTGCCTATCGGATATTTAAGGTATGA
Protein sequence

>MS.gene54980.t1

MAVANAELLLPVGYKFDPTDHELVEYYLTGKILNLPLPTHLFEFDVFQTEPWRLPRDDRFSLEHRKYYFFDIRNRRFENMDARRAGDGEWRIVEWNEDFALSHNLLIGRKNTFVYWIGQGNQALMTQWWMHEFFIISTIHHPARETAVGAYRIFKV