AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54982

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54982.t1 MTR_7g083360 86.014 143 20 0 1 143 1 143 4.10e-95 273
MS.gene54982.t1 MTR_7g083330 63.043 184 60 3 3 178 11 194 1.48e-80 237
MS.gene54982.t1 MTR_7g083370 79.259 135 28 0 1 135 1 135 3.33e-78 229
MS.gene54982.t1 MTR_3g088110 34.868 152 89 5 6 154 3 147 5.93e-22 90.1
MS.gene54982.t1 MTR_3g109340 35.338 133 80 5 7 135 19 149 2.67e-21 89.4
MS.gene54982.t1 MTR_3g109340 35.338 133 80 5 7 135 19 149 2.70e-21 89.4
MS.gene54982.t1 MTR_6g032770 37.692 130 75 4 8 133 16 143 4.47e-21 89.0
MS.gene54982.t1 MTR_7g085260 36.364 132 76 5 8 133 15 144 3.01e-20 86.7
MS.gene54982.t1 MTR_7g085220 31.544 149 89 4 7 144 5 151 1.56e-19 84.3
MS.gene54982.t1 MTR_2157s0010 33.088 136 84 4 3 133 1 134 4.73e-19 82.0
MS.gene54982.t1 MTR_4g081870 31.613 155 96 6 4 154 3 151 4.87e-19 82.0
MS.gene54982.t1 MTR_1g008740 35.821 134 79 5 7 135 10 141 8.29e-19 82.4
MS.gene54982.t1 MTR_1g008740 35.821 134 79 5 7 135 24 155 8.51e-19 82.4
MS.gene54982.t1 MTR_8g059170 31.206 141 88 5 6 143 10 144 9.50e-19 82.0
MS.gene54982.t1 MTR_2g080010 32.353 136 86 4 2 133 7 140 1.08e-18 82.0
MS.gene54982.t1 MTR_3g096920 33.088 136 84 4 6 138 3 134 1.46e-18 81.3
MS.gene54982.t1 MTR_4g089135 34.375 128 78 4 10 133 15 140 1.50e-18 81.6
MS.gene54982.t1 MTR_2g079990 31.206 141 88 5 6 143 12 146 1.75e-18 81.6
MS.gene54982.t1 MTR_2g079990 31.206 141 88 5 6 143 12 146 2.32e-18 81.6
MS.gene54982.t1 MTR_6g084430 35.385 130 78 5 7 133 2 128 2.67e-18 80.9
MS.gene54982.t1 MTR_3g116070 34.132 167 91 8 7 167 20 173 2.73e-18 80.9
MS.gene54982.t1 MTR_2g064090 28.804 184 108 5 6 168 2 183 2.83e-18 80.9
MS.gene54982.t1 MTR_3g096920 33.088 136 84 4 6 138 3 134 3.07e-18 80.5
MS.gene54982.t1 MTR_7g005280 34.586 133 78 6 5 133 10 137 3.70e-18 79.3
MS.gene54982.t1 MTR_8g094580 32.090 134 84 4 8 138 5 134 5.02e-18 79.7
MS.gene54982.t1 MTR_5g076850 33.588 131 83 3 6 133 2 131 1.10e-17 80.1
MS.gene54982.t1 MTR_2g078700 33.594 128 79 5 10 133 23 148 1.71e-17 79.0
MS.gene54982.t1 MTR_5g041940 31.126 151 94 6 8 154 1 145 2.08e-17 77.4
MS.gene54982.t1 MTR_6g012670 33.083 133 83 4 4 133 14 143 2.35e-17 78.2
MS.gene54982.t1 MTR_5g040420 32.374 139 87 5 7 141 12 147 4.25e-17 77.8
MS.gene54982.t1 MTR_3g064580 32.824 131 84 3 6 133 2 131 7.29e-17 77.8
MS.gene54982.t1 MTR_7g011120 32.692 156 99 5 3 155 1 153 1.35e-16 75.9
MS.gene54982.t1 MTR_2g068880 34.532 139 81 6 3 133 14 150 1.58e-16 75.9
MS.gene54982.t1 MTR_4g108760 32.308 130 82 5 7 133 14 140 1.58e-16 76.3
MS.gene54982.t1 MTR_6g477900 33.333 129 82 3 8 133 4 131 1.97e-16 74.7
MS.gene54982.t1 MTR_3g435150 32.308 130 82 5 7 133 13 139 2.13e-16 75.5
MS.gene54982.t1 MTR_3g070030 35.075 134 81 5 3 133 12 142 2.17e-16 75.9
MS.gene54982.t1 MTR_8g099750 30.370 135 86 4 3 133 7 137 3.57e-16 73.6
MS.gene54982.t1 MTR_6g011860 31.852 135 87 3 9 140 14 146 4.84e-16 75.1
MS.gene54982.t1 MTR_2g062730 31.387 137 90 3 1 133 1 137 5.16e-16 74.7
MS.gene54982.t1 MTR_7g011130 33.582 134 83 5 3 133 12 142 5.62e-16 74.7
MS.gene54982.t1 MTR_2g093810 31.333 150 93 6 10 154 14 158 5.63e-16 74.3
MS.gene54982.t1 MTR_5g090970 34.091 132 79 5 7 133 16 144 6.19e-16 74.3
MS.gene54982.t1 MTR_5g090970 34.091 132 78 5 7 133 16 143 1.11e-15 73.6
MS.gene54982.t1 MTR_8g467490 31.884 138 87 5 7 140 12 146 1.39e-15 73.6
MS.gene54982.t1 MTR_2g068920 34.532 139 81 6 2 133 14 149 1.42e-15 72.8
MS.gene54982.t1 MTR_1g090720 32.283 127 82 3 10 133 42 167 1.93e-15 73.6
MS.gene54982.t1 MTR_3g096140 32.836 134 85 4 3 133 1 132 2.31e-15 72.0
MS.gene54982.t1 MTR_7g100990 30.827 133 86 4 5 133 6 136 3.60e-15 72.0
MS.gene54982.t1 MTR_3g096140 32.836 134 85 4 3 133 1 132 6.57e-15 71.6
MS.gene54982.t1 MTR_1g097300 31.746 126 82 3 13 135 22 146 6.85e-15 72.0
MS.gene54982.t1 MTR_1g069805 28.402 169 108 6 1 162 1 163 9.99e-15 70.5
MS.gene54982.t1 MTR_4g035590 29.630 189 110 8 1 172 1 183 3.47e-14 69.7
MS.gene54982.t1 MTR_5g021710 32.031 128 82 4 10 133 8 134 3.50e-14 69.7
MS.gene54982.t1 MTR_4g036030 31.250 128 83 4 10 133 7 133 3.59e-14 69.3
MS.gene54982.t1 MTR_7g097090 31.387 137 88 4 1 133 1 135 3.75e-14 68.9
MS.gene54982.t1 MTR_5g069030 34.646 127 79 3 10 133 25 150 7.26e-14 68.9
MS.gene54982.t1 MTR_8g076110 31.250 128 83 4 10 133 6 132 8.87e-14 68.2
MS.gene54982.t1 MTR_2g064470 28.472 144 97 4 3 144 4 143 1.01e-13 67.8
MS.gene54982.t1 MTR_8g023840 30.070 143 94 4 9 146 10 151 1.03e-13 68.6
MS.gene54982.t1 MTR_5g012080 29.688 128 86 3 10 133 7 134 1.46e-13 67.8
MS.gene54982.t1 MTR_4g134460 29.839 124 82 3 13 133 57 178 1.84e-13 67.8
MS.gene54982.t1 MTR_3g093050 33.071 127 81 3 10 133 22 147 2.05e-13 67.8
MS.gene54982.t1 MTR_3g093050 33.071 127 81 3 10 133 22 147 2.15e-13 67.8
MS.gene54982.t1 MTR_8g023900 29.518 166 109 6 10 168 12 176 4.43e-13 66.6
MS.gene54982.t1 MTR_1g090723 31.343 134 84 5 13 142 11 140 5.82e-13 66.2
MS.gene54982.t1 MTR_1g096430 29.323 133 89 4 5 133 2 133 7.09e-13 65.9
MS.gene54982.t1 MTR_8g063550 30.709 127 84 3 10 133 6 131 7.33e-13 65.9
MS.gene54982.t1 MTR_1g090723 30.370 135 84 5 13 142 11 140 7.43e-13 66.2
MS.gene54982.t1 MTR_8g063550 30.709 127 84 3 10 133 6 131 7.55e-13 65.9
MS.gene54982.t1 MTR_1g090723 30.370 135 84 5 13 142 11 140 7.65e-13 66.2
MS.gene54982.t1 MTR_8g023880 26.573 143 83 5 10 133 7 146 7.95e-13 65.1
MS.gene54982.t1 MTR_8g063550 30.709 127 84 3 10 133 6 131 8.21e-13 65.9
MS.gene54982.t1 MTR_2g086880 30.935 139 89 7 1 136 11 145 1.34e-12 65.5
MS.gene54982.t1 MTR_2g086880 30.935 139 89 7 1 136 11 145 1.35e-12 65.5
MS.gene54982.t1 MTR_3g093040 30.233 129 86 3 8 133 106 233 2.24e-12 64.3
MS.gene54982.t1 MTR_8g023860 29.452 146 89 5 10 146 12 152 4.63e-12 63.5
MS.gene54982.t1 MTR_2g086690 30.065 153 98 4 9 154 3 153 5.88e-12 62.4
MS.gene54982.t1 MTR_3g070040 31.387 137 79 4 3 133 1 128 9.57e-12 62.4
MS.gene54982.t1 MTR_8g023930 27.168 173 112 5 10 173 24 191 1.66e-11 62.0
MS.gene54982.t1 MTR_0036s0150 28.000 125 85 3 13 133 8 131 3.18e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54982.t1 AT5G13180 38.519 135 74 5 3 133 7 136 6.19e-24 94.7
MS.gene54982.t1 AT5G18270 37.209 129 76 4 8 133 19 145 1.09e-21 90.1
MS.gene54982.t1 AT5G18270 36.434 129 77 4 8 133 19 145 1.82e-21 89.7
MS.gene54982.t1 AT3G10500 35.036 137 84 4 10 142 9 144 5.84e-21 89.4
MS.gene54982.t1 AT1G77450 33.758 157 94 5 1 154 1 150 6.50e-21 86.7
MS.gene54982.t1 AT5G04410 35.433 127 78 3 10 133 9 134 6.87e-21 89.4
MS.gene54982.t1 AT5G63790 33.824 136 83 4 6 138 46 177 2.43e-20 86.3
MS.gene54982.t1 AT5G63790 33.824 136 83 4 6 138 56 187 2.54e-20 86.3
MS.gene54982.t1 AT5G08790 33.088 136 84 4 6 138 3 134 3.57e-20 85.5
MS.gene54982.t1 AT1G76420 36.641 131 77 5 7 133 19 147 4.56e-20 85.9
MS.gene54982.t1 AT2G24430 35.878 131 78 5 7 133 13 141 7.67e-20 85.1
MS.gene54982.t1 AT2G24430 35.878 131 78 5 7 133 13 141 7.67e-20 85.1
MS.gene54982.t1 AT3G04070 31.690 142 88 4 10 144 10 149 1.20e-19 84.7
MS.gene54982.t1 AT3G04070 31.690 142 88 4 10 144 10 149 1.72e-19 84.3
MS.gene54982.t1 AT1G61110 33.858 127 79 3 10 133 16 140 4.76e-19 82.8
MS.gene54982.t1 AT1G01720 32.836 134 83 4 8 138 5 134 1.96e-18 80.9
MS.gene54982.t1 AT5G53950 31.013 158 84 6 10 144 17 172 2.24e-18 81.6
MS.gene54982.t1 AT3G29035 34.615 130 79 5 7 133 21 147 2.39e-18 80.9
MS.gene54982.t1 AT5G61430 30.857 175 88 7 7 151 13 184 3.55e-18 80.5
MS.gene54982.t1 AT3G15500 31.206 141 88 5 6 143 10 144 3.77e-18 80.5
MS.gene54982.t1 AT3G10490 34.074 135 85 4 2 133 19 152 8.64e-18 78.2
MS.gene54982.t1 AT5G07680 33.333 132 82 5 5 133 12 140 9.83e-18 79.3
MS.gene54982.t1 AT1G52890 31.206 141 88 5 6 143 10 144 1.01e-17 79.3
MS.gene54982.t1 AT4G27410 31.298 131 84 4 6 133 10 137 1.08e-17 79.0
MS.gene54982.t1 AT5G07680 34.109 129 79 5 8 133 1 126 1.46e-17 78.6
MS.gene54982.t1 AT3G04060 33.333 129 81 4 8 133 18 144 1.95e-17 78.6
MS.gene54982.t1 AT2G33480 34.615 130 74 5 8 133 13 135 2.29e-17 77.4
MS.gene54982.t1 AT2G33480 34.615 130 74 5 8 133 13 135 2.33e-17 77.4
MS.gene54982.t1 AT5G39610 33.088 136 85 5 1 133 11 143 2.46e-17 77.8
MS.gene54982.t1 AT5G17260 32.824 131 84 3 6 133 2 131 2.55e-17 79.0
MS.gene54982.t1 AT3G10480 34.074 135 85 4 2 133 19 152 4.91e-17 78.2
MS.gene54982.t1 AT3G10480 34.074 135 85 4 2 133 19 152 5.02e-17 78.2
MS.gene54982.t1 AT3G15170 32.812 128 80 5 10 133 20 145 5.30e-17 77.0
MS.gene54982.t1 AT3G03200 33.588 131 83 3 6 133 2 131 5.88e-17 77.8
MS.gene54982.t1 AT3G10490 34.074 135 85 4 2 133 19 152 6.57e-17 77.8
MS.gene54982.t1 AT3G17730 33.588 131 83 3 6 133 2 131 7.65e-17 75.9
MS.gene54982.t1 AT3G15510 33.065 124 77 4 14 133 21 142 8.78e-17 77.0
MS.gene54982.t1 AT1G65910 32.061 131 85 3 6 133 2 131 8.79e-17 77.4
MS.gene54982.t1 AT3G18400 31.056 161 103 6 7 162 2 159 1.24e-16 76.3
MS.gene54982.t1 AT1G54330 32.824 131 84 3 6 133 2 131 2.38e-16 75.1
MS.gene54982.t1 AT1G69490 30.827 133 86 4 4 133 3 132 2.92e-16 74.7
MS.gene54982.t1 AT5G39820 29.545 132 87 4 13 140 23 152 5.82e-16 74.3
MS.gene54982.t1 AT1G34180 31.746 126 82 3 13 135 19 143 6.01e-16 75.1
MS.gene54982.t1 AT4G36160 30.657 137 90 4 1 133 5 140 1.05e-15 73.9
MS.gene54982.t1 AT1G34180 31.746 126 82 3 13 135 19 143 1.06e-15 74.3
MS.gene54982.t1 AT2G43000 33.846 130 79 5 7 133 16 141 1.11e-15 73.2
MS.gene54982.t1 AT3G12910 36.090 133 74 5 8 133 20 148 1.13e-15 73.6
MS.gene54982.t1 AT4G36160 30.657 137 90 4 1 133 1 136 1.20e-15 73.9
MS.gene54982.t1 AT4G36160 30.657 137 90 4 1 133 1 136 1.20e-15 73.9
MS.gene54982.t1 AT1G34190 31.387 137 85 4 13 141 19 154 1.24e-15 74.3
MS.gene54982.t1 AT1G26870 31.008 129 81 5 10 133 16 141 1.71e-15 73.6
MS.gene54982.t1 AT4G27410 28.767 146 85 5 6 133 10 154 3.43e-15 72.0
MS.gene54982.t1 AT5G66300 32.812 128 81 4 10 133 12 138 5.38e-15 71.2
MS.gene54982.t1 AT2G27300 34.307 137 85 5 1 135 7 140 9.50e-15 71.2
MS.gene54982.t1 AT4G17980 28.986 138 94 3 10 143 6 143 9.59e-15 70.5
MS.gene54982.t1 AT1G33060 33.858 127 80 4 10 133 24 149 1.12e-14 71.6
MS.gene54982.t1 AT1G33060 33.858 127 80 4 10 133 24 149 1.15e-14 71.2
MS.gene54982.t1 AT5G62380 29.771 131 81 5 10 133 7 133 1.78e-14 70.5
MS.gene54982.t1 AT5G62380 29.771 131 81 5 10 133 7 133 1.78e-14 70.5
MS.gene54982.t1 AT4G17980 28.986 138 94 3 10 143 6 143 2.21e-14 69.7
MS.gene54982.t1 AT1G79580 28.676 136 93 3 2 133 9 144 3.18e-14 69.7
MS.gene54982.t1 AT1G79580 28.676 136 93 3 2 133 9 144 3.18e-14 69.7
MS.gene54982.t1 AT1G79580 28.676 136 93 3 2 133 9 144 3.18e-14 69.7
MS.gene54982.t1 AT1G79580 28.676 136 93 3 2 133 9 144 3.18e-14 69.7
MS.gene54982.t1 AT1G79580 28.676 136 93 3 2 133 9 144 3.18e-14 69.7
MS.gene54982.t1 AT4G35580 33.071 127 81 3 10 133 9 134 5.16e-14 69.3
MS.gene54982.t1 AT5G46590 29.134 127 87 2 10 133 6 132 5.78e-14 68.6
MS.gene54982.t1 AT5G46590 29.134 127 87 2 10 133 6 132 5.95e-14 68.6
MS.gene54982.t1 AT1G34180 29.710 138 81 4 13 135 19 155 7.03e-14 68.9
MS.gene54982.t1 AT2G18060 31.250 128 83 4 10 133 9 135 7.20e-14 68.9
MS.gene54982.t1 AT2G18060 31.250 128 83 4 10 133 9 135 7.20e-14 68.9
MS.gene54982.t1 AT4G35580 33.071 127 81 3 10 133 9 134 7.30e-14 68.9
MS.gene54982.t1 AT4G35580 33.071 127 81 3 10 133 9 134 7.38e-14 68.9
MS.gene54982.t1 AT4G10350 32.353 136 87 4 10 141 9 143 1.05e-13 68.2
MS.gene54982.t1 AT1G34180 29.710 138 81 4 13 135 19 155 1.25e-13 68.2
MS.gene54982.t1 AT1G33280 32.308 130 83 4 10 135 8 136 1.40e-13 67.8
MS.gene54982.t1 AT3G12977 28.148 135 91 4 2 133 10 141 1.91e-13 67.0
MS.gene54982.t1 AT5G24590 31.496 127 84 2 10 133 13 139 3.08e-13 67.0
MS.gene54982.t1 AT5G14000 27.737 137 93 4 3 136 8 141 5.05e-13 63.9
MS.gene54982.t1 AT1G56010 29.412 136 89 4 2 133 11 143 5.39e-13 66.2
MS.gene54982.t1 AT2G02450 28.226 124 84 3 13 133 54 175 7.98e-13 65.9
MS.gene54982.t1 AT2G02450 28.226 124 84 3 13 133 54 175 1.37e-12 65.1
MS.gene54982.t1 AT1G71930 29.688 128 85 4 10 133 9 135 1.48e-12 64.7
MS.gene54982.t1 AT1G71930 29.688 128 85 4 10 133 9 135 1.48e-12 64.7
MS.gene54982.t1 AT5G64060 29.771 131 88 4 6 133 2 131 1.54e-12 65.1
MS.gene54982.t1 AT5G14000 27.737 137 93 4 3 136 8 141 3.26e-12 62.8
MS.gene54982.t1 AT5G04395 28.758 153 93 5 1 137 19 171 3.86e-12 62.0
MS.gene54982.t1 AT1G32870 30.709 127 86 2 10 135 10 135 4.72e-12 63.9
MS.gene54982.t1 AT1G32870 30.709 127 86 2 10 135 10 135 4.89e-12 63.9
MS.gene54982.t1 AT1G32870 30.709 127 86 2 10 135 46 171 5.04e-12 63.5
MS.gene54982.t1 AT1G02230 26.708 161 108 4 11 164 4 161 9.56e-12 62.8
MS.gene54982.t1 AT3G49530 28.346 127 88 2 10 133 13 139 1.78e-11 62.0
MS.gene54982.t1 AT3G49530 28.346 127 88 2 10 133 13 139 1.93e-11 62.0
MS.gene54982.t1 AT5G64530 29.231 130 78 5 8 135 1 118 6.12e-11 58.2
MS.gene54982.t1 AT3G44350 28.244 131 88 3 9 133 4 134 6.27e-11 58.2
MS.gene54982.t1 AT2G46770 25.676 148 104 5 4 146 10 156 8.52e-11 60.1

Find 47 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATCTACCTGCTGGATTC+AGG 0.237662 7.2:+23908852 MS.gene54982:CDS
GAACAGTCGGTTCGAAAATA+TGG 0.258410 7.2:+23909359 MS.gene54982:CDS
GAAAGAGCACATTTGTTTAC+TGG 0.275802 7.2:+23909466 MS.gene54982:CDS
TGCAGGTAATAGGTACTCTT+TGG 0.340271 7.2:+23909305 MS.gene54982:intron
TCAAATTCAGACATGACATT+TGG 0.359524 7.2:-23908941 None:intergenic
ATTGTATTCCTTACCCTAGT+TGG 0.360335 7.2:-23909561 None:intergenic
AGTATTATTTCTTTGATCTC+AGG 0.394952 7.2:+23909337 MS.gene54982:CDS
AATATGGACGCAAGACGAGC+TGG 0.462643 7.2:+23909375 MS.gene54982:CDS
GACGCAAGACGAGCTGGAAA+TGG 0.464716 7.2:+23909381 MS.gene54982:CDS
GCACATTTGTTTACTGGAGT+GGG 0.466777 7.2:+23909472 MS.gene54982:CDS
TGAGTTCATGGAGAGGGAGG+AGG 0.476558 7.2:-23909830 None:intergenic
TCAGACATGACATTTGGAAA+AGG 0.488819 7.2:-23908935 None:intergenic
GAAGAAGGCCACCACACTAA+TGG 0.491907 7.2:+23909787 MS.gene54982:CDS
ACTGAGCCATGGAGGCTGCC+AGG 0.492712 7.2:+23908974 MS.gene54982:CDS
ACATGACATTTGGAAAAGGT+TGG 0.502196 7.2:-23908931 None:intergenic
AAACCTCCTGGCAGCCTCCA+TGG 0.506870 7.2:-23908980 None:intergenic
AGCACATTTGTTTACTGGAG+TGG 0.509000 7.2:+23909471 MS.gene54982:CDS
TGTCAGCTGTTGGTGCCTAT+CGG 0.513489 7.2:+23909734 MS.gene54982:intron
AAATTCATGCATCCACCATC+GGG 0.513722 7.2:-23909517 None:intergenic
TTTGTTTACTGGAGTGGGCA+AGG 0.516244 7.2:+23909477 MS.gene54982:CDS
GAGTTCATGGAGAGGGAGGA+GGG 0.516664 7.2:-23909829 None:intergenic
CAAATTCATGCATCCACCAT+CGG 0.517794 7.2:-23909518 None:intergenic
GCTGAGCTTGATCTACCTGC+TGG 0.518168 7.2:+23908845 MS.gene54982:CDS
GACGAGCTGGAAATGGTGAA+TGG 0.521079 7.2:+23909388 MS.gene54982:CDS
ATCGGATCTTTAAGATATGA+AGG 0.521757 7.2:+23909752 MS.gene54982:CDS
ATAGGTACTCTTTGGAGCAT+AGG 0.529909 7.2:+23909313 MS.gene54982:CDS
AGGTGGCAAAACTGAGAAGA+AGG 0.540768 7.2:+23909772 MS.gene54982:CDS
AGTTCATGGAGAGGGAGGAG+GGG 0.541559 7.2:-23909828 None:intergenic
ATATATTTCAAACTGAGCCA+TGG 0.550284 7.2:+23908963 MS.gene54982:CDS
TGCATCCTCCATTAGTGTGG+TGG 0.554338 7.2:-23909795 None:intergenic
TGAAACATGAGTTCATGGAG+AGG 0.563520 7.2:-23909837 None:intergenic
GAGAGTAGTTGAAAAGAACA+AGG 0.579351 7.2:+23909410 MS.gene54982:CDS
TCTTTGATCTCAGGAACAGT+CGG 0.584323 7.2:+23909346 MS.gene54982:CDS
GGATCAAACCTGAATCCAGC+AGG 0.586690 7.2:-23908860 None:intergenic
ATCGAGCTCTGATGACCCGA+TGG 0.606095 7.2:+23909502 MS.gene54982:CDS
ACATGAGTTCATGGAGAGGG+AGG 0.614894 7.2:-23909833 None:intergenic
GAAACATGAGTTCATGGAGA+GGG 0.632009 7.2:-23909836 None:intergenic
TCAACAAGTTGACGATCGGT+CGG 0.636753 7.2:-23908881 None:intergenic
GAAGGCCACCACACTAATGG+AGG 0.652157 7.2:+23909790 MS.gene54982:CDS
TCATATCTTAAAGATCCGAT+AGG 0.652337 7.2:-23909749 None:intergenic
GAGCTCTGATGACCCGATGG+TGG 0.653753 7.2:+23909505 MS.gene54982:CDS
GCACTCAACAAGTTGACGAT+CGG 0.655422 7.2:-23908885 None:intergenic
TATTTCAAACTGAGCCATGG+AGG 0.675314 7.2:+23908966 MS.gene54982:CDS
ACTTGCATCCTCCATTAGTG+TGG 0.678198 7.2:-23909798 None:intergenic
GAGCCATGGAGGCTGCCAGG+AGG 0.693460 7.2:+23908977 MS.gene54982:CDS
TGGATGCATGAATTTGTCAT+CGG 0.708626 7.2:+23909525 MS.gene54982:CDS
GGATCTTTAAGATATGAAGG+TGG 0.724453 7.2:+23909755 MS.gene54982:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCAATATCAAAAAATTTG+AGG + chr7.2:23909593-23909612 MS.gene54982:intron 15.0%
!!! AAATTTTTTGATATTGATAG+TGG - chr7.2:23909592-23909611 None:intergenic 15.0%
!!! ATATGATGTTTAATTTTATG+TGG + chr7.2:23909217-23909236 MS.gene54982:intron 15.0%
!!! TATGATGTTTAATTTTATGT+GGG + chr7.2:23909218-23909237 MS.gene54982:intron 15.0%
!! TCATAAAGAAAGAGAATAAA+GGG - chr7.2:23909047-23909066 None:intergenic 20.0%
!! TTTATTGATAAAACACACAA+TGG - chr7.2:23909070-23909089 None:intergenic 20.0%
!!! AATTTGTTTTTATATGTTGC+AGG + chr7.2:23909288-23909307 MS.gene54982:intron 20.0%
!!! TTGTCTTGTTTATATTATTC+AGG + chr7.2:23909666-23909685 MS.gene54982:intron 20.0%
! AACATCATATAACCATTTCA+TGG - chr7.2:23909207-23909226 None:intergenic 25.0%
! ACATCATATAACCATTTCAT+GGG - chr7.2:23909206-23909225 None:intergenic 25.0%
! GTCATAAAGAAAGAGAATAA+AGG - chr7.2:23909048-23909067 None:intergenic 25.0%
! TTGAACTCTCAAATTAATGT+AGG + chr7.2:23909711-23909730 MS.gene54982:intron 25.0%
!! AGTATTATTTCTTTGATCTC+AGG + chr7.2:23909337-23909356 MS.gene54982:CDS 25.0%
!! ATTTTCTTAGTGTTCTTTGA+TGG + chr7.2:23909096-23909115 MS.gene54982:intron 25.0%
!! TAGAATTTTTGATCCAACTA+GGG + chr7.2:23909548-23909567 MS.gene54982:CDS 25.0%
!! TTTTATATGTTGCAGGTAAT+AGG + chr7.2:23909295-23909314 MS.gene54982:intron 25.0%
!!! TATTTTTATGTCCCATGAAA+TGG + chr7.2:23909192-23909211 MS.gene54982:intron 25.0%
ATATATTTCAAACTGAGCCA+TGG + chr7.2:23908963-23908982 MS.gene54982:CDS 30.0%
TCAAATTCAGACATGACATT+TGG - chr7.2:23908944-23908963 None:intergenic 30.0%
TCATATCTTAAAGATCCGAT+AGG - chr7.2:23909752-23909771 None:intergenic 30.0%
! GTAGAATTTTTGATCCAACT+AGG + chr7.2:23909547-23909566 MS.gene54982:CDS 30.0%
!! ATCGGATCTTTAAGATATGA+AGG + chr7.2:23909752-23909771 MS.gene54982:CDS 30.0%
!!! TGCTAGTTTTATGTACACTT+TGG + chr7.2:23909636-23909655 MS.gene54982:intron 30.0%
ACATGACATTTGGAAAAGGT+TGG - chr7.2:23908934-23908953 None:intergenic 35.0%
ATTGTATTCCTTACCCTAGT+TGG - chr7.2:23909564-23909583 None:intergenic 35.0%
GAGAGTAGTTGAAAAGAACA+AGG + chr7.2:23909410-23909429 MS.gene54982:CDS 35.0%
TCAGACATGACATTTGGAAA+AGG - chr7.2:23908938-23908957 None:intergenic 35.0%
TGGATGCATGAATTTGTCAT+CGG + chr7.2:23909525-23909544 MS.gene54982:CDS 35.0%
TTGTGTCTTAGACATGCAAT+TGG + chr7.2:23909145-23909164 MS.gene54982:intron 35.0%
! GAAAGAGCACATTTGTTTAC+TGG + chr7.2:23909466-23909485 MS.gene54982:CDS 35.0%
! TTTTTGATCCAACTAGGGTA+AGG + chr7.2:23909553-23909572 MS.gene54982:intron 35.0%
!! GGATCTTTAAGATATGAAGG+TGG + chr7.2:23909755-23909774 MS.gene54982:CDS 35.0%
AAATTCATGCATCCACCATC+GGG - chr7.2:23909520-23909539 None:intergenic 40.0%
AGCACATTTGTTTACTGGAG+TGG + chr7.2:23909471-23909490 MS.gene54982:CDS 40.0%
CAAATTCATGCATCCACCAT+CGG - chr7.2:23909521-23909540 None:intergenic 40.0%
GCACATTTGTTTACTGGAGT+GGG + chr7.2:23909472-23909491 MS.gene54982:CDS 40.0%
GTCTTAGACATGCAATTGGA+GGG + chr7.2:23909149-23909168 MS.gene54982:intron 40.0%
TATTTCAAACTGAGCCATGG+AGG + chr7.2:23908966-23908985 MS.gene54982:CDS 40.0%
TCTTAGACATGCAATTGGAG+GGG + chr7.2:23909150-23909169 MS.gene54982:intron 40.0%
TCTTTGATCTCAGGAACAGT+CGG + chr7.2:23909346-23909365 MS.gene54982:CDS 40.0%
TGCAGGTAATAGGTACTCTT+TGG + chr7.2:23909305-23909324 MS.gene54982:intron 40.0%
TGTCTTAGACATGCAATTGG+AGG + chr7.2:23909148-23909167 MS.gene54982:intron 40.0%
TTAATGTAGGTGTCAGCTGT+TGG + chr7.2:23909724-23909743 MS.gene54982:intron 40.0%
! GTTTTCTTTCACAAACCTCC+TGG - chr7.2:23908995-23909014 None:intergenic 40.0%
!! ATAGGTACTCTTTGGAGCAT+AGG + chr7.2:23909313-23909332 MS.gene54982:CDS 40.0%
!! GAACAGTCGGTTCGAAAATA+TGG + chr7.2:23909359-23909378 MS.gene54982:CDS 40.0%
ACTTGCATCCTCCATTAGTG+TGG - chr7.2:23909801-23909820 None:intergenic 45.0%
AGGTGGCAAAACTGAGAAGA+AGG + chr7.2:23909772-23909791 MS.gene54982:CDS 45.0%
TTGATCTACCTGCTGGATTC+AGG + chr7.2:23908852-23908871 MS.gene54982:CDS 45.0%
TTTGTTTACTGGAGTGGGCA+AGG + chr7.2:23909477-23909496 MS.gene54982:CDS 45.0%
! GCACTCAACAAGTTGACGAT+CGG - chr7.2:23908888-23908907 None:intergenic 45.0%
! GGTTGCAGATTTTCTTCGTC+AGG - chr7.2:23908913-23908932 None:intergenic 45.0%
! TCAACAAGTTGACGATCGGT+CGG - chr7.2:23908884-23908903 None:intergenic 45.0%
!!! AAAATTTAATTTATTCATTT+TGG + chr7.2:23909257-23909276 MS.gene54982:intron 5.0%
!!! AAATTTAATTTATTCATTTT+GGG + chr7.2:23909258-23909277 MS.gene54982:intron 5.0%
AATATGGACGCAAGACGAGC+TGG + chr7.2:23909375-23909394 MS.gene54982:CDS 50.0%
GAAGAAGGCCACCACACTAA+TGG + chr7.2:23909787-23909806 MS.gene54982:CDS 50.0%
GACGAGCTGGAAATGGTGAA+TGG + chr7.2:23909388-23909407 MS.gene54982:CDS 50.0%
GGATCAAACCTGAATCCAGC+AGG - chr7.2:23908863-23908882 None:intergenic 50.0%
TAGACATGCAATTGGAGGGG+AGG + chr7.2:23909153-23909172 MS.gene54982:intron 50.0%
TGCATCCTCCATTAGTGTGG+TGG - chr7.2:23909798-23909817 None:intergenic 50.0%
! TGTCAGCTGTTGGTGCCTAT+CGG + chr7.2:23909734-23909753 MS.gene54982:intron 50.0%
GAAGGCCACCACACTAATGG+AGG + chr7.2:23909790-23909809 MS.gene54982:CDS 55.0%
GACGCAAGACGAGCTGGAAA+TGG + chr7.2:23909381-23909400 MS.gene54982:CDS 55.0%
GCTGAGCTTGATCTACCTGC+TGG + chr7.2:23908845-23908864 MS.gene54982:CDS 55.0%
! ATCGAGCTCTGATGACCCGA+TGG + chr7.2:23909502-23909521 MS.gene54982:CDS 55.0%
AAACCTCCTGGCAGCCTCCA+TGG - chr7.2:23908983-23909002 None:intergenic 60.0%
! GAGCTCTGATGACCCGATGG+TGG + chr7.2:23909505-23909524 MS.gene54982:CDS 60.0%
ACTGAGCCATGGAGGCTGCC+AGG + chr7.2:23908974-23908993 MS.gene54982:CDS 65.0%
GAGCCATGGAGGCTGCCAGG+AGG + chr7.2:23908977-23908996 MS.gene54982:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 23908830 23909847 23908830 ID=MS.gene54982
chr7.2 mRNA 23908830 23909847 23908830 ID=MS.gene54982.t1;Parent=MS.gene54982
chr7.2 exon 23908830 23908998 23908830 ID=MS.gene54982.t1.exon1;Parent=MS.gene54982.t1
chr7.2 CDS 23908830 23908998 23908830 ID=cds.MS.gene54982.t1;Parent=MS.gene54982.t1
chr7.2 exon 23909310 23909569 23909310 ID=MS.gene54982.t1.exon2;Parent=MS.gene54982.t1
chr7.2 CDS 23909310 23909569 23909310 ID=cds.MS.gene54982.t1;Parent=MS.gene54982.t1
chr7.2 exon 23909740 23909847 23909740 ID=MS.gene54982.t1.exon3;Parent=MS.gene54982.t1
chr7.2 CDS 23909740 23909847 23909740 ID=cds.MS.gene54982.t1;Parent=MS.gene54982.t1
Gene Sequence

>MS.gene54982

ATGGCTGTAGCTAATGCTGAGCTTGATCTACCTGCTGGATTCAGGTTTGATCCGACCGATCGTCAACTTGTTGAGTGCTACCTGACGAAGAAAATCTGCAACCAACCTTTTCCAAATGTCATGTCTGAATTTGATATATTTCAAACTGAGCCATGGAGGCTGCCAGGAGGTTTGTGAAAGAAAACAAAAGTTATTTTTCTAATGTTTTTTTTAGCCCTTTATTCTCTTTCTTTATGACCATTGTGTGTTTTATCAATAAAAATCAAATTTTCTTAGTGTTCTTTGATGGATTTTCATCGATCTTGAGAATGAACGTTGTGTCTTAGACATGCAATTGGAGGGGAGGAGACTTAAAAATTCAATATTTTTATGTCCCATGAAATGGTTATATGATGTTTAATTTTATGTGGGTAATTAACAATTTAACAAAATTTAATTTATTCATTTTGGGTCATTTCAATTTGTTTTTATATGTTGCAGGTAATAGGTACTCTTTGGAGCATAGGAAGTATTATTTCTTTGATCTCAGGAACAGTCGGTTCGAAAATATGGACGCAAGACGAGCTGGAAATGGTGAATGGAGAGTAGTTGAAAAGAACAAGGAGTATGCTCATTCAAACAATCAATTGATTGTGAGAAAGAGCACATTTGTTTACTGGAGTGGGCAAGGAAATCGAGCTCTGATGACCCGATGGTGGATGCATGAATTTGTCATCGGTAGAATTTTTGATCCAACTAGGGTAAGGAATACAATATTGACCACTATCAATATCAAAAAATTTGAGGACTTTTTCAGCTGAAAATCATGCTAGTTTTATGTACACTTTGGTTCATTTTTGTCTTGTTTATATTATTCAGGAACTATATATATTCACTGAATTTTGAACTCTCAAATTAATGTAGGTGTCAGCTGTTGGTGCCTATCGGATCTTTAAGATATGAAGGTGGCAAAACTGAGAAGAAGGCCACCACACTAATGGAGGATGCAAGTGTTTCTGCCCCTCCTCCCTCTCCATGA
Protein sequence

>MS.gene54982.t1

MAVANAELDLPAGFRFDPTDRQLVECYLTKKICNQPFPNVMSEFDIFQTEPWRLPGGNRYSLEHRKYYFFDLRNSRFENMDARRAGNGEWRVVEKNKEYAHSNNQLIVRKSTFVYWSGQGNRALMTRWWMHEFVIGRIFDPTRLLVPIGSLRYEGGKTEKKATTLMEDASVSAPPPSP