AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54983

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54983.t1 MTR_7g083330 92.763 152 10 1 1 152 1 151 6.80e-105 298
MS.gene54983.t1 MTR_7g083360 70.803 137 39 1 16 152 8 143 2.59e-70 209
MS.gene54983.t1 MTR_7g083370 74.803 127 31 1 18 144 10 135 8.09e-68 202
MS.gene54983.t1 MTR_6g477900 36.154 130 78 4 16 142 4 131 3.95e-21 86.3
MS.gene54983.t1 MTR_2g064090 35.385 130 79 4 16 142 4 131 4.07e-21 87.8
MS.gene54983.t1 MTR_5g090970 37.778 135 75 6 13 142 14 144 1.05e-19 83.6
MS.gene54983.t1 MTR_4g089135 36.296 135 75 6 18 146 15 144 1.84e-19 83.2
MS.gene54983.t1 MTR_2g068920 36.364 143 74 7 10 142 14 149 4.23e-19 81.6
MS.gene54983.t1 MTR_5g076850 34.615 130 80 4 16 142 4 131 4.33e-19 83.2
MS.gene54983.t1 MTR_2g068880 36.429 140 72 7 13 142 18 150 5.66e-19 81.6
MS.gene54983.t1 MTR_3g064580 34.848 132 77 5 16 142 4 131 8.33e-19 82.4
MS.gene54983.t1 MTR_5g041940 32.593 135 83 6 16 146 1 131 1.91e-18 79.3
MS.gene54983.t1 MTR_2157s0010 30.216 139 89 5 18 151 8 143 2.37e-18 79.3
MS.gene54983.t1 MTR_5g090970 36.296 135 76 6 13 142 14 143 3.34e-18 79.3
MS.gene54983.t1 MTR_7g085220 31.618 136 79 5 18 142 8 140 4.44e-18 79.3
MS.gene54983.t1 MTR_8g094580 30.216 139 89 5 12 147 1 134 4.90e-18 78.6
MS.gene54983.t1 MTR_6g011860 31.507 146 94 4 5 147 2 144 5.52e-18 79.7
MS.gene54983.t1 MTR_3g088110 31.852 135 84 4 16 147 5 134 9.70e-18 77.8
MS.gene54983.t1 MTR_2g080010 34.351 131 75 6 18 142 15 140 1.16e-17 78.6
MS.gene54983.t1 MTR_5g040420 33.094 139 85 6 14 148 11 145 1.29e-17 78.6
MS.gene54983.t1 MTR_3g096920 31.655 139 87 5 12 147 1 134 1.30e-17 77.8
MS.gene54983.t1 MTR_3g070030 35.135 148 84 8 1 142 1 142 1.66e-17 77.8
MS.gene54983.t1 MTR_3g096920 31.655 139 87 5 12 147 1 134 2.01e-17 77.4
MS.gene54983.t1 MTR_1g008740 33.333 132 80 6 18 144 27 155 4.89e-17 76.6
MS.gene54983.t1 MTR_1g008740 33.333 132 80 6 18 144 13 141 5.45e-17 76.6
MS.gene54983.t1 MTR_3g109340 32.877 146 88 7 6 144 7 149 6.51e-17 76.3
MS.gene54983.t1 MTR_3g109340 32.877 146 88 7 6 144 7 149 7.30e-17 76.3
MS.gene54983.t1 MTR_6g032770 32.090 134 84 5 13 142 13 143 9.72e-17 75.9
MS.gene54983.t1 MTR_1g069805 32.353 136 85 5 15 146 7 139 1.12e-16 74.7
MS.gene54983.t1 MTR_4g081870 30.597 134 85 6 17 146 8 137 1.29e-16 74.7
MS.gene54983.t1 MTR_7g005280 35.878 131 74 6 16 142 13 137 1.58e-16 74.3
MS.gene54983.t1 MTR_1g097300 32.192 146 94 4 2 144 3 146 3.02e-16 75.1
MS.gene54983.t1 MTR_5g069030 33.793 145 91 4 1 142 8 150 3.25e-16 74.7
MS.gene54983.t1 MTR_6g012670 34.247 146 88 6 1 142 2 143 5.35e-16 73.6
MS.gene54983.t1 MTR_8g023840 32.143 140 87 5 18 152 11 147 6.78e-16 73.6
MS.gene54983.t1 MTR_2g078700 33.333 129 79 6 18 142 23 148 1.01e-15 73.2
MS.gene54983.t1 MTR_8g023900 32.308 130 81 4 18 142 12 139 1.22e-15 73.2
MS.gene54983.t1 MTR_8g023900 30.303 132 77 5 20 142 195 320 5.88e-11 59.7
MS.gene54983.t1 MTR_8g467490 31.250 144 90 7 12 151 10 148 1.60e-15 72.8
MS.gene54983.t1 MTR_3g093040 32.117 137 88 4 9 142 99 233 1.78e-15 72.0
MS.gene54983.t1 MTR_3g093050 33.571 140 82 5 12 142 10 147 1.85e-15 72.8
MS.gene54983.t1 MTR_3g093050 33.571 140 82 5 12 142 10 147 1.88e-15 72.8
MS.gene54983.t1 MTR_7g097090 32.353 136 85 5 15 146 7 139 6.10e-15 70.5
MS.gene54983.t1 MTR_4g108760 30.827 133 85 6 13 142 12 140 6.57e-15 70.9
MS.gene54983.t1 MTR_7g100990 33.083 133 78 6 16 142 9 136 6.83e-15 70.5
MS.gene54983.t1 MTR_3g116070 33.077 130 79 6 18 142 23 149 7.15e-15 70.5
MS.gene54983.t1 MTR_8g059170 32.824 131 79 6 16 142 12 137 7.74e-15 70.5
MS.gene54983.t1 MTR_2g093810 31.008 129 82 5 18 142 14 139 9.49e-15 70.1
MS.gene54983.t1 MTR_8g023880 28.000 150 85 4 12 142 1 146 1.02e-14 69.3
MS.gene54983.t1 MTR_7g085260 33.835 133 79 6 16 142 15 144 1.28e-14 70.1
MS.gene54983.t1 MTR_6g084430 29.630 135 88 5 18 149 5 135 1.43e-14 69.7
MS.gene54983.t1 MTR_4g036030 30.075 133 87 5 18 146 7 137 1.54e-14 69.7
MS.gene54983.t1 MTR_8g023860 31.343 134 77 5 18 142 12 139 1.61e-14 69.7
MS.gene54983.t1 MTR_8g076110 30.597 134 87 5 18 147 6 137 2.27e-14 68.9
MS.gene54983.t1 MTR_3g435150 31.579 133 84 6 13 142 11 139 2.36e-14 68.9
MS.gene54983.t1 MTR_5g014300 30.935 139 82 5 15 142 3 138 4.58e-14 68.2
MS.gene54983.t1 MTR_8g023930 32.090 134 76 5 18 142 24 151 4.80e-14 68.6
MS.gene54983.t1 MTR_5g012080 27.820 133 91 4 18 146 7 138 5.21e-14 68.2
MS.gene54983.t1 MTR_2g079990 31.538 130 82 5 16 142 14 139 5.23e-14 68.2
MS.gene54983.t1 MTR_2g079990 31.538 130 82 5 16 142 14 139 6.59e-14 67.8
MS.gene54983.t1 MTR_3g096140 31.200 125 81 4 21 142 10 132 7.10e-14 67.4
MS.gene54983.t1 MTR_4g098630 31.618 136 83 4 14 142 2 134 8.49e-14 67.4
MS.gene54983.t1 MTR_2g086880 34.109 129 75 7 21 144 21 144 1.19e-13 67.4
MS.gene54983.t1 MTR_4g101680 30.075 133 87 5 18 146 7 137 1.42e-13 67.0
MS.gene54983.t1 MTR_3g096140 31.200 125 81 4 21 142 10 132 1.56e-13 67.0
MS.gene54983.t1 MTR_1g090720 33.077 130 78 5 18 142 42 167 1.61e-13 67.0
MS.gene54983.t1 MTR_1g090723 32.308 130 74 7 21 142 11 134 1.77e-13 67.0
MS.gene54983.t1 MTR_7g011130 30.137 146 94 7 1 142 1 142 2.17e-13 66.6
MS.gene54983.t1 MTR_1g090723 31.250 128 78 6 21 142 11 134 2.21e-13 66.6
MS.gene54983.t1 MTR_1g090723 31.250 128 78 6 21 142 11 134 2.23e-13 66.6
MS.gene54983.t1 MTR_2g086880 34.109 129 75 7 21 144 21 144 2.45e-13 66.6
MS.gene54983.t1 MTR_8g099750 30.000 130 82 5 17 142 13 137 4.09e-13 64.7
MS.gene54983.t1 MTR_2g062730 30.534 131 86 4 16 142 8 137 4.62e-13 65.5
MS.gene54983.t1 MTR_5g021710 30.233 129 84 5 18 142 8 134 5.88e-13 65.1
MS.gene54983.t1 MTR_1g096430 29.457 129 85 5 18 142 7 133 1.42e-12 63.9
MS.gene54983.t1 MTR_0036s0150 28.571 126 84 4 21 142 8 131 2.68e-12 63.5
MS.gene54983.t1 MTR_4g035590 32.824 131 82 6 18 144 8 136 5.69e-12 62.4
MS.gene54983.t1 MTR_8g063550 30.233 129 85 4 17 142 5 131 1.16e-11 61.6
MS.gene54983.t1 MTR_8g063550 30.233 129 85 4 17 142 5 131 1.20e-11 61.6
MS.gene54983.t1 MTR_8g063550 30.233 129 85 4 17 142 5 131 1.25e-11 61.6
MS.gene54983.t1 MTR_3g070040 32.836 134 72 6 16 142 6 128 2.16e-11 60.8
MS.gene54983.t1 MTR_4g134460 32.000 125 79 4 21 142 57 178 2.91e-11 60.5
MS.gene54983.t1 MTR_3g098810 30.827 133 79 6 17 142 13 139 4.47e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54983.t1 AT5G13180 36.957 138 77 5 9 142 5 136 2.08e-22 89.7
MS.gene54983.t1 AT3G10500 36.719 128 76 4 18 142 9 134 4.40e-22 91.7
MS.gene54983.t1 AT5G04410 35.156 128 78 4 18 142 9 134 4.98e-21 88.6
MS.gene54983.t1 AT3G17730 35.659 129 80 3 16 142 4 131 1.49e-20 84.7
MS.gene54983.t1 AT1G65910 33.846 130 81 4 16 142 4 131 1.13e-19 84.7
MS.gene54983.t1 AT5G17260 34.615 130 80 4 16 142 4 131 1.49e-19 84.3
MS.gene54983.t1 AT3G12910 36.879 141 77 6 13 146 17 152 1.50e-19 83.2
MS.gene54983.t1 AT5G39820 33.108 148 91 6 4 147 7 150 2.29e-19 82.8
MS.gene54983.t1 AT3G03200 35.385 130 79 4 16 142 4 131 3.33e-19 83.2
MS.gene54983.t1 AT2G24430 34.884 129 77 6 18 142 16 141 3.59e-19 82.0
MS.gene54983.t1 AT2G24430 34.884 129 77 6 18 142 16 141 3.59e-19 82.0
MS.gene54983.t1 AT1G54330 37.879 132 73 4 16 142 4 131 3.86e-19 82.0
MS.gene54983.t1 AT1G61110 34.615 130 75 5 18 142 16 140 4.45e-19 82.0
MS.gene54983.t1 AT1G76420 33.108 148 90 7 1 142 3 147 4.88e-19 82.0
MS.gene54983.t1 AT3G04070 32.593 135 81 5 18 145 10 141 6.79e-19 81.6
MS.gene54983.t1 AT2G33480 34.043 141 83 5 5 142 2 135 7.15e-19 80.9
MS.gene54983.t1 AT2G33480 34.043 141 83 5 5 142 2 135 7.59e-19 80.5
MS.gene54983.t1 AT3G04070 32.593 135 81 5 18 145 10 141 1.12e-18 81.3
MS.gene54983.t1 AT1G77450 32.593 135 83 4 16 147 8 137 4.95e-18 78.2
MS.gene54983.t1 AT1G01720 32.593 135 83 4 16 147 5 134 7.79e-18 78.2
MS.gene54983.t1 AT5G08790 31.111 135 85 5 16 147 5 134 9.79e-18 77.8
MS.gene54983.t1 AT5G63790 31.111 135 85 5 16 147 58 187 1.61e-17 77.8
MS.gene54983.t1 AT5G63790 31.111 135 85 5 16 147 48 177 1.61e-17 77.8
MS.gene54983.t1 AT1G26870 35.556 135 78 7 12 142 12 141 2.77e-17 77.8
MS.gene54983.t1 AT5G18270 35.338 133 80 5 13 142 16 145 3.40e-17 77.0
MS.gene54983.t1 AT2G43000 35.338 133 78 6 13 142 14 141 4.38e-17 75.9
MS.gene54983.t1 AT5G18270 34.586 133 81 5 13 142 16 145 8.22e-17 75.9
MS.gene54983.t1 AT3G10490 36.434 129 75 6 18 142 27 152 1.02e-16 74.3
MS.gene54983.t1 AT3G15510 33.600 125 76 5 22 142 21 142 1.02e-16 75.9
MS.gene54983.t1 AT3G15170 32.593 135 84 6 12 142 14 145 1.19e-16 75.1
MS.gene54983.t1 AT2G27300 32.394 142 89 7 6 144 3 140 1.85e-16 75.1
MS.gene54983.t1 AT1G34180 33.071 127 80 4 21 144 19 143 1.94e-16 75.5
MS.gene54983.t1 AT5G53950 33.083 133 82 6 14 142 13 142 2.46e-16 74.7
MS.gene54983.t1 AT3G10480 36.434 129 75 6 18 142 27 152 2.50e-16 75.1
MS.gene54983.t1 AT1G69490 32.031 128 80 5 18 142 9 132 2.53e-16 73.9
MS.gene54983.t1 AT3G10480 36.434 129 75 6 18 142 27 152 2.60e-16 75.1
MS.gene54983.t1 AT5G61430 33.835 133 81 6 13 142 11 139 2.65e-16 74.7
MS.gene54983.t1 AT5G07680 33.846 130 79 6 16 142 1 126 2.87e-16 74.3
MS.gene54983.t1 AT5G14000 28.276 145 95 4 5 145 2 141 2.98e-16 71.6
MS.gene54983.t1 AT1G34180 33.071 127 80 4 21 144 19 143 3.16e-16 74.7
MS.gene54983.t1 AT5G07680 33.083 133 82 6 13 142 12 140 3.25e-16 74.3
MS.gene54983.t1 AT3G29035 33.077 130 80 6 16 142 22 147 3.49e-16 73.9
MS.gene54983.t1 AT1G34190 33.858 127 79 4 21 144 19 143 4.00e-16 74.7
MS.gene54983.t1 AT5G39610 32.414 145 83 7 1 142 11 143 6.17e-16 73.2
MS.gene54983.t1 AT5G46590 30.469 128 85 3 18 142 6 132 7.77e-16 72.8
MS.gene54983.t1 AT5G46590 30.469 128 85 3 18 142 6 132 8.55e-16 72.8
MS.gene54983.t1 AT5G14000 28.276 145 95 4 5 145 2 141 1.13e-15 71.2
MS.gene54983.t1 AT3G10490 35.606 132 78 6 15 142 24 152 1.25e-15 73.2
MS.gene54983.t1 AT5G66300 31.818 132 84 5 15 142 9 138 1.80e-15 72.0
MS.gene54983.t1 AT1G79580 30.534 131 86 4 16 142 15 144 4.53e-15 71.2
MS.gene54983.t1 AT1G79580 30.534 131 86 4 16 142 15 144 4.53e-15 71.2
MS.gene54983.t1 AT1G79580 30.534 131 86 4 16 142 15 144 4.53e-15 71.2
MS.gene54983.t1 AT1G79580 30.534 131 86 4 16 142 15 144 4.53e-15 71.2
MS.gene54983.t1 AT1G79580 30.534 131 86 4 16 142 15 144 4.53e-15 71.2
MS.gene54983.t1 AT3G04060 33.077 130 81 5 16 142 18 144 5.13e-15 70.9
MS.gene54983.t1 AT4G17980 30.147 136 86 5 18 147 6 138 6.22e-15 70.1
MS.gene54983.t1 AT4G17980 29.104 134 90 4 18 147 6 138 1.17e-14 69.7
MS.gene54983.t1 AT3G12977 31.884 138 86 6 18 151 18 151 1.18e-14 69.3
MS.gene54983.t1 AT4G35580 33.594 128 80 4 18 142 9 134 1.27e-14 70.5
MS.gene54983.t1 AT4G35580 33.594 128 80 4 18 142 9 134 1.42e-14 70.1
MS.gene54983.t1 AT4G35580 33.594 128 80 4 18 142 9 134 1.48e-14 70.1
MS.gene54983.t1 AT4G27410 31.538 130 82 5 16 142 12 137 1.65e-14 69.3
MS.gene54983.t1 AT5G62380 28.777 139 93 5 12 146 1 137 1.73e-14 69.7
MS.gene54983.t1 AT5G62380 28.777 139 93 5 12 146 1 137 1.73e-14 69.7
MS.gene54983.t1 AT1G52890 31.538 130 82 5 16 142 12 137 2.42e-14 68.9
MS.gene54983.t1 AT1G33060 31.915 141 91 4 5 142 11 149 4.02e-14 68.9
MS.gene54983.t1 AT1G33060 31.915 141 91 4 5 142 11 149 4.03e-14 68.9
MS.gene54983.t1 AT4G36160 29.630 135 89 5 12 142 8 140 5.51e-14 68.2
MS.gene54983.t1 AT4G36160 29.630 135 89 5 12 142 4 136 5.57e-14 68.2
MS.gene54983.t1 AT4G36160 29.630 135 89 5 12 142 4 136 5.57e-14 68.2
MS.gene54983.t1 AT1G34180 30.216 139 80 5 21 144 19 155 5.80e-14 68.6
MS.gene54983.t1 AT3G15500 30.769 130 83 4 16 142 12 137 8.42e-14 67.4
MS.gene54983.t1 AT1G34180 30.216 139 80 5 21 144 19 155 9.06e-14 67.8
MS.gene54983.t1 AT1G02230 30.714 140 86 4 16 148 1 136 9.23e-14 67.4
MS.gene54983.t1 AT3G18400 32.812 128 79 6 18 142 5 128 1.28e-13 67.0
MS.gene54983.t1 AT1G71930 30.769 130 84 5 17 142 8 135 1.51e-13 66.6
MS.gene54983.t1 AT1G71930 30.769 130 84 5 17 142 8 135 1.51e-13 66.6
MS.gene54983.t1 AT5G09330 33.333 129 81 5 17 142 5 131 2.72e-13 66.6
MS.gene54983.t1 AT5G09330 33.333 129 81 5 17 142 5 131 2.72e-13 66.6
MS.gene54983.t1 AT5G09330 33.333 129 81 5 17 142 5 131 2.72e-13 66.6
MS.gene54983.t1 AT5G09330 33.333 129 81 5 17 142 5 131 2.72e-13 66.6
MS.gene54983.t1 AT5G09330 33.333 129 81 5 17 142 5 131 2.72e-13 66.6
MS.gene54983.t1 AT5G24590 31.250 128 84 3 18 142 13 139 3.19e-13 66.2
MS.gene54983.t1 AT2G18060 28.571 133 89 5 14 142 5 135 4.54e-13 65.5
MS.gene54983.t1 AT2G18060 28.571 133 89 5 14 142 5 135 4.54e-13 65.5
MS.gene54983.t1 AT1G12260 29.323 133 88 5 18 146 7 137 5.68e-13 65.5
MS.gene54983.t1 AT1G12260 29.323 133 88 5 18 146 7 137 8.38e-13 65.1
MS.gene54983.t1 AT1G32510 30.216 139 83 5 15 142 3 138 1.07e-12 64.3
MS.gene54983.t1 AT4G28530 30.405 148 80 6 15 142 7 151 2.07e-12 63.5
MS.gene54983.t1 AT1G62700 27.612 134 91 5 17 146 6 137 3.04e-12 63.5
MS.gene54983.t1 AT1G62700 27.612 134 91 5 17 146 6 137 3.04e-12 63.5
MS.gene54983.t1 AT4G27410 27.586 145 85 5 16 142 12 154 3.20e-12 63.2
MS.gene54983.t1 AT3G49530 28.906 128 87 3 18 142 13 139 4.45e-12 62.8
MS.gene54983.t1 AT1G33280 31.298 131 84 5 18 144 8 136 1.04e-11 61.6
MS.gene54983.t1 AT3G49530 28.906 128 87 3 18 142 13 139 1.37e-11 61.6
MS.gene54983.t1 AT1G52880 29.365 126 82 4 22 142 21 144 1.46e-11 61.2
MS.gene54983.t1 AT4G10350 31.783 129 82 5 18 142 9 135 1.54e-11 61.2
MS.gene54983.t1 AT1G32770 29.197 137 91 5 10 142 8 142 1.95e-11 60.8
MS.gene54983.t1 AT5G64530 30.534 131 76 6 16 144 1 118 2.73e-11 58.5
MS.gene54983.t1 AT3G44350 29.851 134 87 4 15 142 2 134 2.89e-11 58.5
MS.gene54983.t1 AT1G32870 30.282 142 95 4 4 144 33 171 3.70e-11 60.5
MS.gene54983.t1 AT5G64530 30.534 131 76 6 16 144 1 118 3.81e-11 58.5
MS.gene54983.t1 AT1G32870 30.657 137 91 4 9 144 2 135 5.40e-11 59.7
MS.gene54983.t1 AT1G32870 30.657 137 91 4 9 144 2 135 5.79e-11 59.7
MS.gene54983.t1 AT4G01540 29.861 144 93 6 16 152 2 144 7.69e-11 59.3

Find 28 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATTACCTGTTGGATTC+AGG 0.250804 7.2:+23928606 MS.gene54983:CDS
GAAAGAACACATTTGTTTAC+TGG 0.255170 7.2:+23929101 MS.gene54983:CDS
GGTACTATTTCTTTGATATC+AGG 0.295817 7.2:+23928972 MS.gene54983:CDS
GAACCGTAGGTTCGAAAATA+TGG 0.299363 7.2:+23928994 MS.gene54983:CDS
AAAGCATAGTGAGCTAGTTT+TGG 0.338409 7.2:+23928574 MS.gene54983:CDS
TCGAATTCTGACAGGACATT+TGG 0.373254 7.2:-23928695 None:intergenic
GGATCAAACCTGAATCCAAC+AGG 0.478056 7.2:-23928614 None:intergenic
ACAGGACATTTGGAAGAGGT+TGG 0.514459 7.2:-23928685 None:intergenic
AATATGGATGCAAGAAGAGC+TGG 0.518416 7.2:+23929010 MS.gene54983:CDS
GAGCCATGGAAGCTGCCAAA+AGG 0.533677 7.2:+23928731 MS.gene54983:CDS
GAAGAGCTGGAAATGGTGAA+TGG 0.539470 7.2:+23929023 MS.gene54983:CDS
TTTGTTTACTGGAGAATGCA+AGG 0.541628 7.2:+23929112 MS.gene54983:CDS
TCTACAAGCTCATGATCAGT+TGG 0.547810 7.2:-23928635 None:intergenic
GAAATACATCGAATTCTGAC+AGG 0.551455 7.2:-23928703 None:intergenic
GGACCTCTTCAAAGCATATG+AGG 0.554252 7.2:+23928951 MS.gene54983:CDS
TCTGACAGGACATTTGGAAG+AGG 0.561916 7.2:-23928689 None:intergenic
ATGTATTTCAAACTGAGCCA+TGG 0.561975 7.2:+23928717 MS.gene54983:CDS
GATGGTATGAAATTACCTGT+TGG 0.565216 7.2:+23928599 MS.gene54983:CDS
GATGCAAGAAGAGCTGGAAA+TGG 0.566075 7.2:+23929016 MS.gene54983:CDS
GTACCTCATATGCTTTGAAG+AGG 0.594155 7.2:-23928954 None:intergenic
TCTTTGATATCAGGAACCGT+AGG 0.597083 7.2:+23928981 MS.gene54983:CDS
GTGAATGGAGAATATATGAG+AGG 0.620604 7.2:+23929038 MS.gene54983:CDS
TTGTAGATTATTATCTGACG+AGG 0.656467 7.2:+23928651 MS.gene54983:CDS
AGGATGCATGAATTTGTCAT+CGG 0.657194 7.2:+23929163 MS.gene54983:CDS
GTCAAGCTCTGATGACACAA+TGG 0.657620 7.2:+23929140 MS.gene54983:CDS
GAGAATATATGAGAGGAACG+AGG 0.682363 7.2:+23929045 MS.gene54983:CDS
AAGCTCTGATGACACAATGG+AGG 0.685488 7.2:+23929143 MS.gene54983:CDS
GTTTACTGGAGAATGCAAGG+AGG 0.781160 7.2:+23929115 MS.gene54983:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATAAGAAAAAGAATGAA+GGG - chr7.2:23928772-23928791 None:intergenic 20.0%
!! CCATCATAAGATTAATTAAA+GGG - chr7.2:23928865-23928884 None:intergenic 20.0%
!! TCCATCATAAGATTAATTAA+AGG - chr7.2:23928866-23928885 None:intergenic 20.0%
!!! AACACAAGGAAAATTTTAAA+AGG - chr7.2:23928834-23928853 None:intergenic 20.0%
!!! ACACAAGGAAAATTTTAAAA+GGG - chr7.2:23928833-23928852 None:intergenic 20.0%
! CCCTTTAATTAATCTTATGA+TGG + chr7.2:23928862-23928881 MS.gene54983:intron 25.0%
! GATCATAAGAAAAAGAATGA+AGG - chr7.2:23928773-23928792 None:intergenic 25.0%
!! CTATAATTTCACAAACCTTT+TGG - chr7.2:23928749-23928768 None:intergenic 25.0%
!!! ATTTGTTTTCTGCAGATAAT+AGG + chr7.2:23928930-23928949 MS.gene54983:intron 25.0%
GAAAGAACACATTTGTTTAC+TGG + chr7.2:23929101-23929120 MS.gene54983:CDS 30.0%
TTGTAGATTATTATCTGACG+AGG + chr7.2:23928651-23928670 MS.gene54983:CDS 30.0%
! GGTACTATTTCTTTGATATC+AGG + chr7.2:23928972-23928991 MS.gene54983:CDS 30.0%
AAAGGGTTTCAAAGAACACA+AGG - chr7.2:23928848-23928867 None:intergenic 35.0%
AGGATGCATGAATTTGTCAT+CGG + chr7.2:23929163-23929182 MS.gene54983:CDS 35.0%
ATGTATTTCAAACTGAGCCA+TGG + chr7.2:23928717-23928736 MS.gene54983:CDS 35.0%
GAAATACATCGAATTCTGAC+AGG - chr7.2:23928706-23928725 None:intergenic 35.0%
GATGGTATGAAATTACCTGT+TGG + chr7.2:23928599-23928618 MS.gene54983:CDS 35.0%
GTGAATGGAGAATATATGAG+AGG + chr7.2:23929038-23929057 MS.gene54983:CDS 35.0%
TGAAATTACCTGTTGGATTC+AGG + chr7.2:23928606-23928625 MS.gene54983:CDS 35.0%
TTTGTTTACTGGAGAATGCA+AGG + chr7.2:23929112-23929131 MS.gene54983:CDS 35.0%
! CATCCATATTTTCGAACCTA+CGG - chr7.2:23929000-23929019 None:intergenic 35.0%
!! AAAGCATAGTGAGCTAGTTT+TGG + chr7.2:23928574-23928593 MS.gene54983:CDS 35.0%
AATATGGATGCAAGAAGAGC+TGG + chr7.2:23929010-23929029 MS.gene54983:CDS 40.0%
GAACCGTAGGTTCGAAAATA+TGG + chr7.2:23928994-23929013 MS.gene54983:CDS 40.0%
GAGAATATATGAGAGGAACG+AGG + chr7.2:23929045-23929064 MS.gene54983:CDS 40.0%
TCGAATTCTGACAGGACATT+TGG - chr7.2:23928698-23928717 None:intergenic 40.0%
TCTACAAGCTCATGATCAGT+TGG - chr7.2:23928638-23928657 None:intergenic 40.0%
TCTTTGATATCAGGAACCGT+AGG + chr7.2:23928981-23929000 MS.gene54983:CDS 40.0%
!! GTACCTCATATGCTTTGAAG+AGG - chr7.2:23928957-23928976 None:intergenic 40.0%
!!! AGTGAGCTAGTTTTGGAAGA+TGG + chr7.2:23928581-23928600 MS.gene54983:CDS 40.0%
ACAGGACATTTGGAAGAGGT+TGG - chr7.2:23928688-23928707 None:intergenic 45.0%
GAAGAGCTGGAAATGGTGAA+TGG + chr7.2:23929023-23929042 MS.gene54983:CDS 45.0%
GATGCAAGAAGAGCTGGAAA+TGG + chr7.2:23929016-23929035 MS.gene54983:CDS 45.0%
GGACCTCTTCAAAGCATATG+AGG + chr7.2:23928951-23928970 MS.gene54983:CDS 45.0%
GGATCAAACCTGAATCCAAC+AGG - chr7.2:23928617-23928636 None:intergenic 45.0%
GTTTACTGGAGAATGCAAGG+AGG + chr7.2:23929115-23929134 MS.gene54983:CDS 45.0%
TCTGACAGGACATTTGGAAG+AGG - chr7.2:23928692-23928711 None:intergenic 45.0%
! AAACCTTTTGGCAGCTTCCA+TGG - chr7.2:23928737-23928756 None:intergenic 45.0%
! AAGCTCTGATGACACAATGG+AGG + chr7.2:23929143-23929162 MS.gene54983:CDS 45.0%
! GTCAAGCTCTGATGACACAA+TGG + chr7.2:23929140-23929159 MS.gene54983:CDS 45.0%
GAGCCATGGAAGCTGCCAAA+AGG + chr7.2:23928731-23928750 MS.gene54983:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 23928560 23929207 23928560 ID=MS.gene54983
chr7.2 mRNA 23928560 23929207 23928560 ID=MS.gene54983.t1;Parent=MS.gene54983
chr7.2 exon 23928560 23928752 23928560 ID=MS.gene54983.t1.exon1;Parent=MS.gene54983.t1
chr7.2 CDS 23928560 23928752 23928560 ID=cds.MS.gene54983.t1;Parent=MS.gene54983.t1
chr7.2 exon 23928945 23929207 23928945 ID=MS.gene54983.t1.exon2;Parent=MS.gene54983.t1
chr7.2 CDS 23928945 23929207 23928945 ID=cds.MS.gene54983.t1;Parent=MS.gene54983.t1
Gene Sequence

>MS.gene54983

ATGGCTGCTGATGAAAAGCATAGTGAGCTAGTTTTGGAAGATGGTATGAAATTACCTGTTGGATTCAGGTTTGATCCAACTGATCATGAGCTTGTAGATTATTATCTGACGAGGAAAGTCTGTAACCAACCTCTTCCAAATGTCCTGTCAGAATTCGATGTATTTCAAACTGAGCCATGGAAGCTGCCAAAAGGTTTGTGAAATTATAGCCCTTCATTCTTTTTCTTATGATCATAGTGTTTTATCAATAAAAATCAAATTTTTACATGACCCTTTTAAAATTTTCCTTGTGTTCTTTGAAACCCTTTAATTAATCTTATGATGGATTTTAATTAATCTTGTGAATGAACATTGTGTTTTAGACAATCTAATTTGTTTTCTGCAGATAATAGGACCTCTTCAAAGCATATGAGGTACTATTTCTTTGATATCAGGAACCGTAGGTTCGAAAATATGGATGCAAGAAGAGCTGGAAATGGTGAATGGAGAATATATGAGAGGAACGAGGAGTTTGCTCTTTCAAACAATCAATTGATTGCGAGAAAGAACACATTTGTTTACTGGAGAATGCAAGGAGGAAGTCAAGCTCTGATGACACAATGGAGGATGCATGAATTTGTCATCGGTACAATTTTTCATCAAACTAAG
Protein sequence

>MS.gene54983.t1

MAADEKHSELVLEDGMKLPVGFRFDPTDHELVDYYLTRKVCNQPLPNVLSEFDVFQTEPWKLPKDNRTSSKHMRYYFFDIRNRRFENMDARRAGNGEWRIYERNEEFALSNNQLIARKNTFVYWRMQGGSQALMTQWRMHEFVIGTIFHQTK