Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55077.t1 | XP_003624327.1 | 95.6 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 1.20E-54 | 222.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55077.t1 | Q8VZG8 | 52.9 | 87 | 36 | 2 | 1 | 86 | 955 | 1037 | 4.2e-16 | 85.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55077.t1 | G7KU95 | 95.6 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 8.6e-55 | 222.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55077.t1 | MTR_7g081780 | 95.614 | 114 | 5 | 0 | 1 | 114 | 947 | 1060 | 7.04e-72 | 231 |
| MS.gene55077.t1 | MTR_7g081480 | 85.714 | 112 | 16 | 0 | 1 | 112 | 949 | 1060 | 1.67e-62 | 205 |
| MS.gene55077.t1 | MTR_7g481630 | 76.106 | 113 | 26 | 1 | 1 | 112 | 80 | 192 | 1.45e-57 | 176 |
| MS.gene55077.t1 | MTR_7g481590 | 76.106 | 113 | 26 | 1 | 1 | 112 | 203 | 315 | 1.19e-55 | 175 |
| MS.gene55077.t1 | MTR_7g081570 | 76.991 | 113 | 25 | 1 | 1 | 112 | 939 | 1051 | 5.57e-54 | 181 |
| MS.gene55077.t1 | MTR_7g081410 | 74.336 | 113 | 28 | 1 | 1 | 112 | 946 | 1058 | 1.89e-52 | 176 |
| MS.gene55077.t1 | MTR_7g081410 | 74.336 | 113 | 28 | 1 | 1 | 112 | 826 | 938 | 2.18e-52 | 176 |
| MS.gene55077.t1 | MTR_7g081720 | 53.509 | 114 | 51 | 1 | 1 | 112 | 964 | 1077 | 3.51e-32 | 119 |
| MS.gene55077.t1 | MTR_5g085700 | 53.571 | 112 | 52 | 0 | 2 | 113 | 921 | 1032 | 2.35e-31 | 116 |
| MS.gene55077.t1 | MTR_1g039080 | 52.525 | 99 | 46 | 1 | 1 | 98 | 192 | 290 | 3.24e-28 | 104 |
| MS.gene55077.t1 | MTR_8g445960 | 48.000 | 100 | 51 | 1 | 1 | 99 | 63 | 162 | 3.74e-28 | 100 |
| MS.gene55077.t1 | MTR_8g044940 | 48.000 | 100 | 51 | 1 | 1 | 99 | 63 | 162 | 3.74e-28 | 100 |
| MS.gene55077.t1 | MTR_1g038890 | 52.525 | 99 | 46 | 1 | 1 | 98 | 1033 | 1131 | 1.39e-27 | 105 |
| MS.gene55077.t1 | MTR_1g039310 | 47.368 | 114 | 56 | 2 | 3 | 112 | 1051 | 1164 | 1.12e-26 | 103 |
| MS.gene55077.t1 | MTR_7g045510 | 54.348 | 92 | 40 | 1 | 2 | 93 | 1091 | 1180 | 3.39e-26 | 101 |
| MS.gene55077.t1 | MTR_7g039330 | 54.348 | 92 | 40 | 1 | 2 | 93 | 1189 | 1278 | 5.58e-26 | 101 |
| MS.gene55077.t1 | MTR_7g007540 | 44.737 | 114 | 58 | 2 | 1 | 113 | 1 | 110 | 8.53e-25 | 91.3 |
| MS.gene55077.t1 | MTR_7g007560 | 46.491 | 114 | 56 | 2 | 1 | 113 | 653 | 762 | 1.07e-24 | 97.4 |
| MS.gene55077.t1 | MTR_1g039240 | 48.454 | 97 | 46 | 1 | 1 | 93 | 1058 | 1154 | 6.28e-24 | 95.5 |
| MS.gene55077.t1 | MTR_7g009940 | 44.248 | 113 | 58 | 2 | 3 | 114 | 700 | 808 | 1.97e-23 | 94.0 |
| MS.gene55077.t1 | MTR_7g045710 | 50.000 | 94 | 42 | 1 | 2 | 95 | 972 | 1060 | 2.11e-23 | 94.0 |
| MS.gene55077.t1 | MTR_0400s0040 | 48.454 | 97 | 46 | 1 | 1 | 93 | 1058 | 1154 | 2.16e-23 | 94.0 |
| MS.gene55077.t1 | MTR_0365s0030 | 48.454 | 97 | 46 | 1 | 1 | 93 | 1058 | 1154 | 2.16e-23 | 94.0 |
| MS.gene55077.t1 | MTR_7g446160 | 53.684 | 95 | 41 | 2 | 1 | 93 | 887 | 980 | 2.45e-23 | 93.6 |
| MS.gene55077.t1 | MTR_7g051040 | 44.348 | 115 | 55 | 2 | 1 | 108 | 843 | 955 | 4.46e-23 | 92.8 |
| MS.gene55077.t1 | MTR_7g050990 | 51.546 | 97 | 43 | 2 | 1 | 93 | 819 | 915 | 6.19e-23 | 92.4 |
| MS.gene55077.t1 | MTR_7g045860 | 47.917 | 96 | 48 | 1 | 2 | 97 | 984 | 1077 | 1.19e-22 | 91.7 |
| MS.gene55077.t1 | MTR_0400s0030 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 1.32e-22 | 91.7 |
| MS.gene55077.t1 | MTR_0365s0020 | 48.485 | 99 | 50 | 1 | 1 | 98 | 791 | 889 | 1.32e-22 | 91.7 |
| MS.gene55077.t1 | MTR_7g007630 | 44.737 | 114 | 58 | 2 | 1 | 113 | 666 | 775 | 3.76e-22 | 90.1 |
| MS.gene55077.t1 | MTR_1g040525 | 45.263 | 95 | 48 | 2 | 1 | 91 | 767 | 861 | 4.93e-22 | 90.1 |
| MS.gene55077.t1 | MTR_7g009970 | 43.966 | 116 | 56 | 3 | 1 | 113 | 669 | 778 | 8.03e-22 | 89.4 |
| MS.gene55077.t1 | MTR_1g040545 | 46.316 | 95 | 47 | 2 | 1 | 91 | 907 | 1001 | 9.73e-22 | 89.0 |
| MS.gene55077.t1 | MTR_7g407130 | 44.248 | 113 | 58 | 2 | 1 | 112 | 696 | 804 | 2.82e-21 | 87.8 |
| MS.gene55077.t1 | MTR_1g040615 | 45.263 | 95 | 48 | 2 | 1 | 91 | 2024 | 2118 | 6.93e-21 | 86.7 |
| MS.gene55077.t1 | MTR_1g040615 | 47.872 | 94 | 41 | 3 | 1 | 88 | 1025 | 1116 | 2.89e-20 | 85.1 |
| MS.gene55077.t1 | MTR_1g039220 | 48.958 | 96 | 43 | 3 | 1 | 91 | 1032 | 1126 | 8.13e-21 | 86.7 |
| MS.gene55077.t1 | MTR_7g407090 | 45.614 | 114 | 57 | 2 | 1 | 113 | 651 | 760 | 6.22e-20 | 84.0 |
| MS.gene55077.t1 | MTR_7g010000 | 42.241 | 116 | 58 | 3 | 1 | 113 | 746 | 855 | 1.23e-19 | 83.2 |
| MS.gene55077.t1 | MTR_4g037015 | 47.312 | 93 | 45 | 2 | 1 | 93 | 777 | 865 | 1.38e-19 | 82.8 |
| MS.gene55077.t1 | MTR_7g013680 | 41.880 | 117 | 59 | 3 | 1 | 114 | 619 | 729 | 1.39e-19 | 82.8 |
| MS.gene55077.t1 | MTR_7g028432 | 47.312 | 93 | 45 | 2 | 1 | 93 | 853 | 941 | 1.57e-19 | 82.8 |
| MS.gene55077.t1 | MTR_7g007620 | 46.491 | 114 | 56 | 2 | 1 | 113 | 620 | 729 | 2.44e-19 | 82.4 |
| MS.gene55077.t1 | MTR_4g037720 | 44.086 | 93 | 48 | 1 | 1 | 93 | 848 | 936 | 2.53e-19 | 82.0 |
| MS.gene55077.t1 | MTR_7g096980 | 40.206 | 97 | 57 | 1 | 1 | 97 | 1108 | 1203 | 3.61e-18 | 79.0 |
| MS.gene55077.t1 | MTR_0491s0030 | 44.737 | 114 | 58 | 2 | 1 | 113 | 704 | 813 | 4.89e-17 | 75.5 |
| MS.gene55077.t1 | MTR_8g047160 | 52.874 | 87 | 36 | 2 | 1 | 86 | 531 | 613 | 7.06e-17 | 75.1 |
| MS.gene55077.t1 | MTR_8g047230 | 44.737 | 114 | 58 | 2 | 1 | 113 | 875 | 984 | 8.79e-17 | 75.1 |
| MS.gene55077.t1 | MTR_7g013350 | 43.689 | 103 | 49 | 3 | 1 | 100 | 295 | 391 | 1.28e-16 | 74.3 |
| MS.gene55077.t1 | MTR_8g047210 | 44.737 | 114 | 58 | 2 | 1 | 113 | 768 | 877 | 1.01e-15 | 72.0 |
| MS.gene55077.t1 | MTR_8g047220 | 44.737 | 114 | 58 | 2 | 1 | 113 | 831 | 940 | 1.05e-15 | 72.0 |
| MS.gene55077.t1 | MTR_7g446190 | 45.238 | 84 | 39 | 2 | 1 | 80 | 815 | 895 | 2.88e-15 | 70.5 |
| MS.gene55077.t1 | MTR_7g010010 | 37.069 | 116 | 45 | 3 | 1 | 113 | 616 | 706 | 1.45e-13 | 65.9 |
| MS.gene55077.t1 | MTR_7g022160 | 36.449 | 107 | 63 | 2 | 1 | 104 | 909 | 1013 | 1.46e-13 | 65.9 |
| MS.gene55077.t1 | MTR_2g070020 | 38.000 | 100 | 51 | 2 | 1 | 93 | 987 | 1082 | 1.70e-13 | 65.5 |
| MS.gene55077.t1 | MTR_7g022050 | 34.545 | 110 | 67 | 2 | 1 | 107 | 534 | 641 | 5.21e-12 | 61.2 |
| MS.gene55077.t1 | MTR_3g093930 | 35.294 | 102 | 61 | 3 | 1 | 99 | 879 | 978 | 5.78e-12 | 61.2 |
| MS.gene55077.t1 | MTR_3g047890 | 34.906 | 106 | 59 | 2 | 3 | 102 | 360 | 461 | 1.19e-11 | 60.1 |
| MS.gene55077.t1 | MTR_4g058710 | 38.384 | 99 | 51 | 3 | 8 | 98 | 419 | 515 | 3.01e-11 | 59.3 |
| MS.gene55077.t1 | MTR_2g073630 | 37.037 | 108 | 59 | 4 | 3 | 105 | 807 | 910 | 3.19e-11 | 58.9 |
| MS.gene55077.t1 | MTR_7g007580 | 38.318 | 107 | 57 | 3 | 10 | 113 | 3 | 103 | 7.18e-11 | 55.5 |
| MS.gene55077.t1 | MTR_1g079520 | 34.211 | 114 | 60 | 4 | 1 | 104 | 946 | 1054 | 9.42e-11 | 57.8 |
| MS.gene55077.t1 | MTR_1g047670 | 35.000 | 100 | 56 | 3 | 1 | 93 | 989 | 1086 | 9.82e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55077.t1 | AT4G08850 | 51.163 | 86 | 39 | 1 | 1 | 86 | 955 | 1037 | 1.90e-20 | 85.5 |
| MS.gene55077.t1 | AT1G35710 | 47.253 | 91 | 34 | 4 | 1 | 86 | 1031 | 1112 | 4.30e-16 | 73.2 |
| MS.gene55077.t1 | AT3G28040 | 33.645 | 107 | 66 | 2 | 1 | 104 | 908 | 1012 | 2.87e-12 | 62.0 |
| MS.gene55077.t1 | AT1G17230 | 38.000 | 100 | 51 | 3 | 1 | 93 | 977 | 1072 | 5.02e-12 | 61.6 |
| MS.gene55077.t1 | AT1G17230 | 38.000 | 100 | 51 | 3 | 1 | 93 | 977 | 1072 | 6.15e-12 | 61.2 |
| MS.gene55077.t1 | AT5G48940 | 36.458 | 96 | 54 | 2 | 1 | 93 | 972 | 1063 | 6.34e-12 | 61.2 |
| MS.gene55077.t1 | AT2G33170 | 37.624 | 101 | 51 | 3 | 1 | 93 | 1003 | 1099 | 1.04e-11 | 60.5 |
| MS.gene55077.t1 | AT2G33170 | 37.624 | 101 | 51 | 3 | 1 | 93 | 1003 | 1099 | 1.04e-11 | 60.5 |
| MS.gene55077.t1 | AT4G25390 | 37.755 | 98 | 51 | 2 | 3 | 93 | 530 | 624 | 2.05e-11 | 59.7 |
| MS.gene55077.t1 | AT4G31110 | 32.710 | 107 | 64 | 3 | 5 | 105 | 612 | 716 | 4.75e-11 | 58.5 |
| MS.gene55077.t1 | AT4G31110 | 32.710 | 107 | 64 | 3 | 5 | 105 | 634 | 738 | 4.76e-11 | 58.5 |
| MS.gene55077.t1 | AT1G09440 | 35.354 | 99 | 50 | 3 | 3 | 93 | 336 | 428 | 7.30e-11 | 58.2 |
| MS.gene55077.t1 | AT1G09440 | 35.354 | 99 | 50 | 3 | 3 | 93 | 336 | 428 | 7.30e-11 | 58.2 |
| MS.gene55077.t1 | AT4G31100 | 35.556 | 90 | 51 | 2 | 5 | 89 | 625 | 712 | 8.36e-11 | 57.8 |
Find 23 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGGCTGATGATACATTAT+TGG | 0.288645 | 7.2:-25346107 | MS.gene55077:CDS |
| ACTTGCTTTGGAAATCATAA+AGG | 0.335311 | 7.2:-25346182 | MS.gene55077:CDS |
| AAATGTGATGTATACAGTTT+TGG | 0.382117 | 7.2:-25346208 | MS.gene55077:CDS |
| CTTGCTTTGGAAATCATAAA+GGG | 0.419654 | 7.2:-25346181 | MS.gene55077:CDS |
| CAACTTCACGCGATCAAACT+AGG | 0.448143 | 7.2:-25345917 | MS.gene55077:CDS |
| TCAGCCAATGGCCTTGTAGA+TGG | 0.448395 | 7.2:+25346121 | None:intergenic |
| TGGAGCAGGAAAATCACCTT+TGG | 0.448518 | 7.2:-25345945 | MS.gene55077:CDS |
| GTTGGACGAGAACGTGGCTC+TGG | 0.460252 | 7.2:+25345992 | None:intergenic |
| ATCATAAAGGGAGAACATCC+TGG | 0.483511 | 7.2:-25346169 | MS.gene55077:CDS |
| TATAATGAAATGAGATCTCC+AGG | 0.486322 | 7.2:+25346151 | None:intergenic |
| GATCAAGTATGTAAGATGCT+TGG | 0.514611 | 7.2:-25345965 | MS.gene55077:CDS |
| AAGATAACCTCCTCATCGAT+TGG | 0.516703 | 7.2:+25346046 | None:intergenic |
| GTCACCATCTACAAGGCCAT+TGG | 0.519981 | 7.2:-25346125 | MS.gene55077:CDS |
| GTATGTAAGATGCTTGGAGC+AGG | 0.525016 | 7.2:-25345959 | MS.gene55077:CDS |
| CATCTTACATACTTGATCCA+TGG | 0.528171 | 7.2:+25345970 | None:intergenic |
| TATATTTGTCACCATCTACA+AGG | 0.550499 | 7.2:-25346132 | MS.gene55077:CDS |
| TTACATACTTGATCCATGGT+TGG | 0.555375 | 7.2:+25345974 | None:intergenic |
| GCCACGTTCTCGTCCAACCA+TGG | 0.605254 | 7.2:-25345987 | MS.gene55077:CDS |
| TCCATGGTTGGACGAGAACG+TGG | 0.627661 | 7.2:+25345986 | None:intergenic |
| TATGAAACCAATCGATGAGG+AGG | 0.628111 | 7.2:-25346053 | MS.gene55077:CDS |
| AATAATGTATCATCAGCCAA+TGG | 0.642314 | 7.2:+25346109 | None:intergenic |
| GCGTGAAGTTGATCTTCCAA+AGG | 0.654089 | 7.2:+25345929 | None:intergenic |
| AGTTATGAAACCAATCGATG+AGG | 0.678211 | 7.2:-25346056 | MS.gene55077:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGTGATGTATACAGTTT+TGG | - | chr7.2:25345916-25345935 | MS.gene55077:CDS | 25.0% |
| !!! | ATTGGTTTCATAACTTTTTG+AGG | + | chr7.2:25346063-25346082 | None:intergenic | 25.0% |
| AATAATGTATCATCAGCCAA+TGG | + | chr7.2:25346018-25346037 | None:intergenic | 30.0% | |
| ATTGGCTGATGATACATTAT+TGG | - | chr7.2:25346017-25346036 | MS.gene55077:CDS | 30.0% | |
| TATAATGAAATGAGATCTCC+AGG | + | chr7.2:25345976-25345995 | None:intergenic | 30.0% | |
| TATATTTGTCACCATCTACA+AGG | - | chr7.2:25345992-25346011 | MS.gene55077:CDS | 30.0% | |
| !! | ACTTGCTTTGGAAATCATAA+AGG | - | chr7.2:25345942-25345961 | MS.gene55077:CDS | 30.0% |
| !! | CTTGCTTTGGAAATCATAAA+GGG | - | chr7.2:25345943-25345962 | MS.gene55077:CDS | 30.0% |
| AGTTATGAAACCAATCGATG+AGG | - | chr7.2:25346068-25346087 | MS.gene55077:CDS | 35.0% | |
| CATCTTACATACTTGATCCA+TGG | + | chr7.2:25346157-25346176 | None:intergenic | 35.0% | |
| GATCAAGTATGTAAGATGCT+TGG | - | chr7.2:25346159-25346178 | MS.gene55077:CDS | 35.0% | |
| TTACATACTTGATCCATGGT+TGG | + | chr7.2:25346153-25346172 | None:intergenic | 35.0% | |
| AAGATAACCTCCTCATCGAT+TGG | + | chr7.2:25346081-25346100 | None:intergenic | 40.0% | |
| ATCATAAAGGGAGAACATCC+TGG | - | chr7.2:25345955-25345974 | MS.gene55077:CDS | 40.0% | |
| TATGAAACCAATCGATGAGG+AGG | - | chr7.2:25346071-25346090 | MS.gene55077:CDS | 40.0% | |
| !!! | CAGTTTTGGAGTACTTGCTT+TGG | - | chr7.2:25345930-25345949 | MS.gene55077:CDS | 40.0% |
| CAACTTCACGCGATCAAACT+AGG | - | chr7.2:25346207-25346226 | MS.gene55077:CDS | 45.0% | |
| GTATGTAAGATGCTTGGAGC+AGG | - | chr7.2:25346165-25346184 | MS.gene55077:CDS | 45.0% | |
| TGGAGCAGGAAAATCACCTT+TGG | - | chr7.2:25346179-25346198 | MS.gene55077:CDS | 45.0% | |
| ! | GCGTGAAGTTGATCTTCCAA+AGG | + | chr7.2:25346198-25346217 | None:intergenic | 45.0% |
| GTCACCATCTACAAGGCCAT+TGG | - | chr7.2:25345999-25346018 | MS.gene55077:CDS | 50.0% | |
| !! | TCAGCCAATGGCCTTGTAGA+TGG | + | chr7.2:25346006-25346025 | None:intergenic | 50.0% |
| TCCATGGTTGGACGAGAACG+TGG | + | chr7.2:25346141-25346160 | None:intergenic | 55.0% | |
| GCCACGTTCTCGTCCAACCA+TGG | - | chr7.2:25346137-25346156 | MS.gene55077:CDS | 60.0% | |
| GTTGGACGAGAACGTGGCTC+TGG | + | chr7.2:25346135-25346154 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 25345901 | 25346245 | 25345901 | ID=MS.gene55077 |
| chr7.2 | mRNA | 25345901 | 25346245 | 25345901 | ID=MS.gene55077.t1;Parent=MS.gene55077 |
| chr7.2 | exon | 25345901 | 25346245 | 25345901 | ID=MS.gene55077.t1.exon1;Parent=MS.gene55077.t1 |
| chr7.2 | CDS | 25345901 | 25346245 | 25345901 | ID=cds.MS.gene55077.t1;Parent=MS.gene55077.t1 |
| Gene Sequence |
| Protein sequence |
