AlfalfaGEDB | MS.gene55080

M. sativa cultivar XinJiangDaYe / MS.gene55080

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004672 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55080.t1	RHN47205.1	91.2	114	10	0	1	114	487	600	1.00E-50	209.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55080.t1	Q8VZG8	46.7	90	45	1	1	90	955	1041	3.6e-15	82.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55080.t1	G7KTF7	91.2	114	10	0	1	114	939	1052	7.5e-51	209.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55080.t1	MTR_7g481630	90.351	114	11	0	1	114	80	193	2.71e-72	213
MS.gene55080.t1	MTR_7g481590	90.351	114	11	0	1	114	203	316	1.83e-70	213
MS.gene55080.t1	MTR_7g081570	91.228	114	10	0	1	114	939	1052	6.90e-67	217
MS.gene55080.t1	MTR_7g081410	87.719	114	14	0	1	114	946	1059	7.51e-64	209
MS.gene55080.t1	MTR_7g081410	87.719	114	14	0	1	114	826	939	8.16e-64	208
MS.gene55080.t1	MTR_7g081480	83.333	114	18	1	1	114	949	1061	7.03e-60	197
MS.gene55080.t1	MTR_7g081780	77.876	113	24	1	1	113	947	1058	4.15e-54	181
MS.gene55080.t1	MTR_7g081720	56.897	116	47	2	1	114	964	1078	1.41e-35	128
MS.gene55080.t1	MTR_5g085700	60.204	98	39	0	2	99	921	1018	2.33e-32	119
MS.gene55080.t1	MTR_1g039080	55.340	103	45	1	1	102	192	294	1.46e-31	113
MS.gene55080.t1	MTR_8g445960	53.535	99	45	1	1	98	63	161	6.80e-31	107
MS.gene55080.t1	MTR_8g044940	53.535	99	45	1	1	98	63	161	6.80e-31	107
MS.gene55080.t1	MTR_1g038890	57.576	99	41	1	1	98	1033	1131	7.57e-31	115
MS.gene55080.t1	MTR_7g045510	58.696	92	36	1	2	93	1091	1180	1.83e-29	111
MS.gene55080.t1	MTR_7g039330	58.696	92	36	1	2	93	1189	1278	4.03e-29	110
MS.gene55080.t1	MTR_1g039310	56.701	97	41	1	3	98	1051	1147	5.17e-29	109
MS.gene55080.t1	MTR_0400s0030	54.545	99	44	1	1	98	791	889	6.98e-27	103
MS.gene55080.t1	MTR_0365s0020	54.545	99	44	1	1	98	791	889	6.98e-27	103
MS.gene55080.t1	MTR_7g045710	54.839	93	37	1	2	94	972	1059	9.01e-27	103
MS.gene55080.t1	MTR_7g007540	50.495	101	45	2	1	100	1	97	3.91e-26	94.7
MS.gene55080.t1	MTR_1g040545	52.525	99	43	2	1	95	907	1005	1.60e-25	99.8
MS.gene55080.t1	MTR_1g040525	53.684	95	40	2	1	91	767	861	1.72e-25	99.8
MS.gene55080.t1	MTR_7g446160	55.319	94	41	1	1	93	887	980	2.48e-25	99.4
MS.gene55080.t1	MTR_1g039240	50.505	99	45	1	1	95	1058	1156	2.63e-25	99.4
MS.gene55080.t1	MTR_7g007560	52.475	101	43	2	1	100	653	749	3.08e-25	99.0
MS.gene55080.t1	MTR_0400s0040	50.505	99	45	1	1	95	1058	1156	3.87e-25	99.0
MS.gene55080.t1	MTR_0365s0030	50.505	99	45	1	1	95	1058	1156	3.87e-25	99.0
MS.gene55080.t1	MTR_1g040615	55.319	94	34	3	1	88	1025	1116	4.67e-25	98.6
MS.gene55080.t1	MTR_1g040615	50.505	99	45	2	1	95	2024	2122	5.40e-24	95.5
MS.gene55080.t1	MTR_7g045860	51.042	96	45	1	2	97	984	1077	4.69e-25	98.6
MS.gene55080.t1	MTR_1g039220	52.525	99	43	2	1	95	1032	1130	1.00e-24	97.8
MS.gene55080.t1	MTR_7g050990	51.515	99	44	2	1	95	819	917	4.73e-24	95.5
MS.gene55080.t1	MTR_7g051040	44.444	117	59	2	1	112	843	958	1.03e-23	94.7
MS.gene55080.t1	MTR_7g009970	50.485	103	42	3	1	100	669	765	1.57e-23	94.4
MS.gene55080.t1	MTR_7g007630	50.495	101	45	2	1	100	666	762	5.19e-23	92.8
MS.gene55080.t1	MTR_7g010000	49.515	103	43	3	1	100	746	842	1.42e-22	91.7
MS.gene55080.t1	MTR_7g407130	49.505	101	46	2	1	100	696	792	1.46e-22	91.3
MS.gene55080.t1	MTR_7g009940	50.000	102	40	3	3	100	700	794	2.23e-22	90.9
MS.gene55080.t1	MTR_7g013680	47.573	103	45	3	1	100	619	715	1.56e-20	85.5
MS.gene55080.t1	MTR_7g407090	48.515	101	47	2	1	100	651	747	1.71e-20	85.5
MS.gene55080.t1	MTR_4g037720	48.421	95	41	2	1	93	848	936	3.07e-20	84.7
MS.gene55080.t1	MTR_4g037015	46.392	97	44	2	1	95	777	867	6.27e-20	84.0
MS.gene55080.t1	MTR_7g028432	46.392	97	44	2	1	95	853	943	8.04e-20	83.6
MS.gene55080.t1	MTR_7g096980	37.168	113	70	1	1	113	1108	1219	8.99e-20	83.6
MS.gene55080.t1	MTR_7g007620	50.495	101	45	2	1	100	620	716	1.73e-19	82.8
MS.gene55080.t1	MTR_7g013350	46.602	103	46	3	1	100	295	391	5.08e-19	80.9
MS.gene55080.t1	MTR_0491s0030	55.319	94	33	3	1	91	704	791	1.11e-18	80.5
MS.gene55080.t1	MTR_8g047230	55.319	94	33	3	1	91	875	962	1.99e-18	79.7
MS.gene55080.t1	MTR_8g047160	56.180	89	30	3	1	86	531	613	3.72e-18	79.0
MS.gene55080.t1	MTR_8g047220	54.348	92	37	2	1	91	831	918	5.56e-18	78.6
MS.gene55080.t1	MTR_8g047210	54.348	92	37	2	1	91	768	855	5.59e-18	78.2
MS.gene55080.t1	MTR_7g446190	48.148	81	41	1	1	80	815	895	5.48e-16	72.8
MS.gene55080.t1	MTR_7g010010	41.748	103	32	3	1	100	616	693	1.30e-15	71.6
MS.gene55080.t1	MTR_7g007580	41.489	94	46	3	10	100	3	90	5.33e-13	60.8
MS.gene55080.t1	MTR_7g022160	36.893	103	60	2	1	100	909	1009	5.36e-13	64.3
MS.gene55080.t1	MTR_7g022050	37.624	101	58	2	1	98	534	632	1.31e-12	63.2
MS.gene55080.t1	MTR_2g070020	36.000	100	53	2	1	93	987	1082	3.31e-12	62.0
MS.gene55080.t1	MTR_3g093930	34.314	102	62	3	1	99	879	978	3.88e-12	61.6
MS.gene55080.t1	MTR_0007s0390	40.594	101	49	4	2	94	559	656	9.40e-12	60.5
MS.gene55080.t1	MTR_1g080440	31.000	100	66	1	1	97	888	987	6.19e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55080.t1	AT4G08850	46.667	90	45	1	1	90	955	1041	1.26e-19	83.2
MS.gene55080.t1	AT1G35710	44.681	94	48	2	1	94	1031	1120	1.57e-17	77.0
MS.gene55080.t1	AT1G17230	38.000	100	51	2	1	93	977	1072	8.90e-13	63.5
MS.gene55080.t1	AT1G17230	38.000	100	51	2	1	93	977	1072	8.91e-13	63.5
MS.gene55080.t1	AT3G28040	34.951	103	62	2	1	100	908	1008	1.91e-12	62.8
MS.gene55080.t1	AT4G25390	36.735	98	52	2	3	93	530	624	7.68e-12	60.8
MS.gene55080.t1	AT5G62710	39.583	96	49	3	3	93	491	582	3.97e-11	58.9
MS.gene55080.t1	AT5G62710	39.583	96	49	3	3	93	457	548	4.19e-11	58.9
MS.gene55080.t1	AT1G07650	35.780	109	49	4	3	100	645	743	4.90e-11	58.5
MS.gene55080.t1	AT1G07650	35.780	109	49	4	3	100	864	962	7.08e-11	58.2
MS.gene55080.t1	AT1G07650	35.780	109	49	4	3	100	832	930	7.20e-11	58.2
MS.gene55080.t1	AT1G07650	35.780	109	49	4	3	100	858	956	7.21e-11	58.2
MS.gene55080.t1	AT3G56370	34.314	102	62	3	1	99	855	954	7.55e-11	58.2
MS.gene55080.t1	AT5G56790	34.375	96	57	2	2	93	567	660	9.08e-11	57.8

Find 25 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTGCTTTGGAAATCATAA+TGG	0.280746	7.2:-25406996	MS.gene55080:CDS
CTTGCTTTGGAAATCATAAT+GGG	0.363369	7.2:-25406995	MS.gene55080:CDS
AAATGTGATGTATACAGTTT+TGG	0.382117	7.2:-25407022	MS.gene55080:CDS
GGTTATCTTGATTGCAAGGT+TGG	0.406732	7.2:-25406846	MS.gene55080:CDS
TTAGCCGTTGGTCTTGTAGA+TGG	0.411133	7.2:+25406935	None:intergenic
AAGATAACCTCCTCATCAAT+TGG	0.413575	7.2:+25406860	None:intergenic
GTATCTAAGATGCTTGCAAT+AGG	0.435731	7.2:-25406773	MS.gene55080:CDS
ATCATAATGGGAAAGCATCC+TGG	0.452740	7.2:-25406983	MS.gene55080:CDS
TGCAATAGGAAAATCACCTT+TGG	0.457045	7.2:-25406759	MS.gene55080:CDS
AATAATGAAATGAGATCTCC+AGG	0.486322	7.2:+25406965	None:intergenic
AATAGGAAAATCACCTTTGG+TGG	0.490438	7.2:-25406756	MS.gene55080:CDS
CAACTTCACATGGTCAGACT+AGG	0.524082	7.2:-25406728	MS.gene55080:CDS
ATTGGTTTCATAACATGTTG+AGG	0.526760	7.2:+25406878	None:intergenic
AGGAGGTTATCTTGATTGCA+AGG	0.533682	7.2:-25406850	MS.gene55080:CDS
CATCTTAGATACTTGATCCA+TGG	0.535258	7.2:+25406784	None:intergenic
TGAAGTTGCTTTCCCACCAA+AGG	0.564942	7.2:+25406743	None:intergenic
TCCACGTTCTCGACCAACCA+TGG	0.573192	7.2:-25406801	MS.gene55080:CDS
TCCATGGTTGGTCGAGAACG+TGG	0.573752	7.2:+25406800	None:intergenic
TTAGATACTTGATCCATGGT+TGG	0.575363	7.2:+25406788	None:intergenic
TATGAAACCAATTGATGAGG+AGG	0.601236	7.2:-25406867	MS.gene55080:CDS
GGTGGGAAAGCAACTTCACA+TGG	0.603086	7.2:-25406738	MS.gene55080:CDS
GTCACCATCTACAAGACCAA+CGG	0.611431	7.2:-25406939	MS.gene55080:CDS
AACAACATATCATTAGCCGT+TGG	0.627701	7.2:+25406923	None:intergenic
ATAGGAAAATCACCTTTGGT+GGG	0.631051	7.2:-25406755	MS.gene55080:CDS
TGTTATGAAACCAATTGATG+AGG	0.646181	7.2:-25406870	MS.gene55080:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATGTGATGTATACAGTTT+TGG	-	chr7.2:25406730-25406749	MS.gene55080:CDS	25.0%
	AATAATGAAATGAGATCTCC+AGG	+	chr7.2:25406790-25406809	None:intergenic	30.0%
	TGTTATGAAACCAATTGATG+AGG	-	chr7.2:25406882-25406901	MS.gene55080:CDS	30.0%
!	ATTGGTTTCATAACATGTTG+AGG	+	chr7.2:25406877-25406896	None:intergenic	30.0%
!!	ACTTGCTTTGGAAATCATAA+TGG	-	chr7.2:25406756-25406775	MS.gene55080:CDS	30.0%
!!	CTTGCTTTGGAAATCATAAT+GGG	-	chr7.2:25406757-25406776	MS.gene55080:CDS	30.0%
	AACAACATATCATTAGCCGT+TGG	+	chr7.2:25406832-25406851	None:intergenic	35.0%
	AAGATAACCTCCTCATCAAT+TGG	+	chr7.2:25406895-25406914	None:intergenic	35.0%
	AATAGGAAAATCACCTTTGG+TGG	-	chr7.2:25406996-25407015	MS.gene55080:CDS	35.0%
	ATAGGAAAATCACCTTTGGT+GGG	-	chr7.2:25406997-25407016	MS.gene55080:CDS	35.0%
	CATCTTAGATACTTGATCCA+TGG	+	chr7.2:25406971-25406990	None:intergenic	35.0%
	TATGAAACCAATTGATGAGG+AGG	-	chr7.2:25406885-25406904	MS.gene55080:CDS	35.0%
	TGCAATAGGAAAATCACCTT+TGG	-	chr7.2:25406993-25407012	MS.gene55080:CDS	35.0%
	TTAGATACTTGATCCATGGT+TGG	+	chr7.2:25406967-25406986	None:intergenic	35.0%
!	GTATCTAAGATGCTTGCAAT+AGG	-	chr7.2:25406979-25406998	MS.gene55080:CDS	35.0%
	AGGAGGTTATCTTGATTGCA+AGG	-	chr7.2:25406902-25406921	MS.gene55080:CDS	40.0%
	GGTTATCTTGATTGCAAGGT+TGG	-	chr7.2:25406906-25406925	MS.gene55080:CDS	40.0%
!!	ATCATAATGGGAAAGCATCC+TGG	-	chr7.2:25406769-25406788	MS.gene55080:CDS	40.0%
!!!	CAGTTTTGGAGTACTTGCTT+TGG	-	chr7.2:25406744-25406763	MS.gene55080:CDS	40.0%
	CAACTTCACATGGTCAGACT+AGG	-	chr7.2:25407024-25407043	MS.gene55080:CDS	45.0%
	GTCACCATCTACAAGACCAA+CGG	-	chr7.2:25406813-25406832	MS.gene55080:CDS	45.0%
!	TGAAGTTGCTTTCCCACCAA+AGG	+	chr7.2:25407012-25407031	None:intergenic	45.0%
!!	TTAGCCGTTGGTCTTGTAGA+TGG	+	chr7.2:25406820-25406839	None:intergenic	45.0%
	GGTGGGAAAGCAACTTCACA+TGG	-	chr7.2:25407014-25407033	MS.gene55080:CDS	50.0%
	TCCACGTTCTCGACCAACCA+TGG	-	chr7.2:25406951-25406970	MS.gene55080:CDS	55.0%
!	TCCATGGTTGGTCGAGAACG+TGG	+	chr7.2:25406955-25406974	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	25406715	25407059	25406715	ID=MS.gene55080
chr7.2	mRNA	25406715	25407059	25406715	ID=MS.gene55080.t1;Parent=MS.gene55080
chr7.2	exon	25406715	25407059	25406715	ID=MS.gene55080.t1.exon1;Parent=MS.gene55080.t1
chr7.2	CDS	25406715	25407059	25406715	ID=cds.MS.gene55080.t1;Parent=MS.gene55080.t1

Gene Sequence	>MS.gene55080 ATGGAAGTGAATGAGAAATGTGATGTATACAGTTTTGGAGTACTTGCTTTGGAAATCATAATGGGAAAGCATCCTGGAGATCTCATTTCATTATTTTTGTCACCATCTACAAGACCAACGGCTAATGATATGTTGTTGATAGATGTTTTAGATCAAAGACCTCAACATGTTATGAAACCAATTGATGAGGAGGTTATCTTGATTGCAAGGTTGGCATTTGCTTGCTTGAATCAAAATCCACGTTCTCGACCAACCATGGATCAAGTATCTAAGATGCTTGCAATAGGAAAATCACCTTTGGTGGGAAAGCAACTTCACATGGTCAGACTAGGACATCTCCACTAA
Protein sequence	>MS.gene55080.t1 MEVNEKCDVYSFGVLALEIIMGKHPGDLISLFLSPSTRPTANDMLLIDVLDQRPQHVMKPIDEEVILIARLAFACLNQNPRSRPTMDQVSKMLAIGKSPLVGKQLHMVRLGHLH