AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55223

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55223.t1 MTR_0558s0020 89.068 311 19 1 1 311 1 296 0.0 578
MS.gene55223.t1 MTR_1g083640 93.706 286 18 0 1 286 1 286 0.0 566
MS.gene55223.t1 MTR_8g446020 77.170 311 16 2 1 311 1 256 8.40e-169 473
MS.gene55223.t1 MTR_2g014100 88.142 253 21 2 1 252 1 245 4.22e-162 456
MS.gene55223.t1 MTR_6g090505 88.608 237 27 0 81 317 1 237 2.31e-154 441
MS.gene55223.t1 MTR_0337s0040 73.455 275 19 2 1 275 1 221 9.78e-137 391
MS.gene55223.t1 MTR_3g008240 83.945 218 20 1 1 218 1 203 9.02e-131 375
MS.gene55223.t1 MTR_4g040430 84.404 218 14 2 1 218 1 198 2.93e-130 374
MS.gene55223.t1 MTR_5g071590 86.885 183 21 1 1 183 1 180 4.41e-116 337
MS.gene55223.t1 MTR_1g055240 94.366 142 8 0 107 248 3 144 1.09e-96 286
MS.gene55223.t1 MTR_0019s0290 88.889 153 14 1 100 249 8 160 9.18e-96 284
MS.gene55223.t1 MTR_1281s0010 95.413 109 5 0 35 143 1 109 4.27e-71 219
MS.gene55223.t1 MTR_6g051425 30.818 318 189 6 56 357 22 324 3.68e-41 149
MS.gene55223.t1 MTR_8g046060 79.167 96 11 2 1 95 91 178 7.09e-39 139
MS.gene55223.t1 MTR_4g008820 76.842 95 7 1 1 95 125 204 1.38e-36 142
MS.gene55223.t1 MTR_1g070355 24.756 307 210 5 3 307 24 311 1.32e-21 97.4
MS.gene55223.t1 MTR_0337s0050 80.556 36 7 0 275 310 14 49 2.40e-14 68.2
MS.gene55223.t1 MTR_0402s0040 25.946 185 118 4 65 249 40 205 4.46e-14 74.3
MS.gene55223.t1 MTR_1g013060 30.093 216 132 8 56 269 29 227 6.18e-14 71.2
MS.gene55223.t1 MTR_4g035820 23.019 265 179 5 56 315 80 324 2.81e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55223.t1 AT4G38180 31.329 316 192 6 50 361 72 366 1.79e-41 157
MS.gene55223.t1 AT3G06250 27.607 326 172 7 39 361 178 442 1.03e-31 128
MS.gene55223.t1 AT3G06250 27.607 326 172 7 39 361 178 442 1.03e-31 128
MS.gene55223.t1 AT3G06250 27.607 326 172 7 39 361 178 442 1.03e-31 128
MS.gene55223.t1 AT5G28530 27.523 327 207 6 56 370 60 368 1.27e-30 125
MS.gene55223.t1 AT5G28530 27.523 327 207 6 56 370 60 368 1.27e-30 125
MS.gene55223.t1 AT5G28530 27.523 327 207 6 56 370 60 368 1.42e-30 124
MS.gene55223.t1 AT4G15090 28.763 299 192 6 66 363 66 344 7.54e-28 117
MS.gene55223.t1 AT4G15090 28.763 299 192 6 66 363 66 344 7.54e-28 117
MS.gene55223.t1 AT4G15090 28.763 299 192 6 66 363 66 344 7.54e-28 117
MS.gene55223.t1 AT4G15090 28.763 299 192 6 66 363 66 344 7.54e-28 117
MS.gene55223.t1 AT4G15090 28.763 299 192 6 66 363 66 344 7.54e-28 117
MS.gene55223.t1 AT5G18960 25.758 330 180 9 37 361 197 466 5.02e-27 114
MS.gene55223.t1 AT5G18960 25.758 330 180 9 37 361 197 466 5.02e-27 114
MS.gene55223.t1 AT1G80010 27.950 322 191 10 57 366 101 393 1.80e-26 112
MS.gene55223.t1 AT2G27110 34.507 142 93 0 229 370 181 322 5.09e-26 111
MS.gene55223.t1 AT2G27110 34.507 142 93 0 229 370 181 322 5.09e-26 111
MS.gene55223.t1 AT2G27110 34.507 142 93 0 229 370 36 177 7.28e-26 110
MS.gene55223.t1 AT2G32250 26.901 342 182 9 24 363 13 288 6.37e-25 108
MS.gene55223.t1 AT2G32250 26.901 342 182 9 24 363 13 288 6.63e-25 108
MS.gene55223.t1 AT2G32250 26.901 342 182 9 24 363 13 288 6.63e-25 108
MS.gene55223.t1 AT2G32250 26.901 342 182 9 24 363 13 288 6.63e-25 108
MS.gene55223.t1 AT2G32250 26.901 342 182 9 24 363 13 288 6.86e-25 108
MS.gene55223.t1 AT2G32250 26.901 342 182 9 24 363 13 288 6.86e-25 108
MS.gene55223.t1 AT1G76320 24.919 309 200 6 55 361 1 279 7.76e-25 107
MS.gene55223.t1 AT1G76320 24.919 309 200 6 55 361 1 279 7.99e-25 107
MS.gene55223.t1 AT1G76320 24.919 309 200 6 55 361 1 279 7.99e-25 107
MS.gene55223.t1 AT1G76320 25.081 307 198 6 57 361 3 279 1.01e-24 107
MS.gene55223.t1 AT2G32250 27.508 309 158 8 57 363 3 247 1.67e-24 106
MS.gene55223.t1 AT2G32250 27.508 309 158 8 57 363 3 247 1.72e-24 106
MS.gene55223.t1 AT1G10240 25.000 304 200 8 65 361 62 344 2.76e-24 105
MS.gene55223.t1 AT1G10240 25.000 304 200 8 65 361 62 344 2.76e-24 105
MS.gene55223.t1 AT4G38170 30.168 179 94 4 251 402 24 198 9.89e-24 103
MS.gene55223.t1 AT3G22170 23.734 316 206 6 48 360 67 350 8.15e-22 98.6
MS.gene55223.t1 AT3G22170 23.734 316 206 6 48 360 67 350 8.15e-22 98.6
MS.gene55223.t1 AT1G52520 25.890 309 193 8 57 363 91 365 2.06e-21 97.4
MS.gene55223.t1 AT4G19990 23.410 393 228 11 57 422 27 373 7.26e-19 89.7
MS.gene55223.t1 AT4G19990 23.155 393 229 10 57 422 41 387 8.04e-19 89.4
MS.gene55223.t1 AT4G19990 23.410 393 228 11 57 422 27 373 8.39e-19 89.4
MS.gene55223.t1 AT4G19990 23.410 393 228 11 57 422 27 373 8.39e-19 89.4
MS.gene55223.t1 AT4G19990 23.410 393 228 11 57 422 27 373 9.02e-19 89.4
MS.gene55223.t1 AT4G19990 23.410 393 228 11 57 422 27 373 9.02e-19 89.4
MS.gene55223.t1 AT4G19990 27.907 215 124 5 235 422 106 316 5.27e-17 84.0

Find 52 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATTTCAGCTGCCTTTAT+TGG 0.156227 5.2:+30596414 MS.gene55223:intron
AATCGTCTGTGGTTTCTTAT+TGG 0.195298 5.2:-30596541 None:intergenic
TCTGTTGTTGGCCTGTTGTT+AGG 0.292589 5.2:-30595335 None:intergenic
TTACTTCTCGAAATGATCTA+AGG 0.311482 5.2:-30595526 None:intergenic
CAACTAGATTATTCTCTATT+CGG 0.323831 5.2:+30595842 MS.gene55223:CDS
AATAAGGTTTCTCTGTTAGT+TGG 0.336164 5.2:-30596818 None:intergenic
GCCGATATTTATTGTTTGTT+CGG 0.341758 5.2:-30595892 None:intergenic
ATGATTGTCAAAACCAATAA+AGG 0.357876 5.2:-30596427 None:intergenic
AACAATAAATATCGGCCATT+AGG 0.388497 5.2:+30595899 MS.gene55223:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 5.2:+30595891 MS.gene55223:CDS
GAGAACGTCTTATGAAGTTA+TGG 0.394356 5.2:+30595601 MS.gene55223:CDS
GAAGGCCTACCTCAGACTTT+AGG 0.402909 5.2:+30596787 MS.gene55223:CDS
GTCTTATGAAGTTATGGGTC+AGG 0.415385 5.2:+30595607 MS.gene55223:CDS
GAAGTTATGGGTCAGGGAGT+TGG 0.426260 5.2:+30595614 MS.gene55223:CDS
CGTTATGTTTGTAACAAAGA+AGG 0.427485 5.2:+30595287 MS.gene55223:CDS
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 5.2:+30595539 MS.gene55223:CDS
TCCATGATTCTTTATTGCTA+GGG 0.444266 5.2:-30595124 None:intergenic
TTCCATGATTCTTTATTGCT+AGG 0.445442 5.2:-30595125 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 5.2:+30595404 MS.gene55223:CDS
ATGTGTATAAGATATATAGA+TGG 0.452819 5.2:+30595456 MS.gene55223:CDS
ACATCAACTTCATCTGATAG+TGG 0.459514 5.2:+30594999 MS.gene55223:CDS
GCTCTTATAAATTCTAAAGC+TGG 0.462891 5.2:+30595569 MS.gene55223:CDS
ACCTGTGGACTGTCTGTTGT+TGG 0.465317 5.2:-30595347 None:intergenic
AGAACGTCTTATGAAGTTAT+GGG 0.466325 5.2:+30595602 MS.gene55223:CDS
GTTTACTTAATCCTAACAAC+AGG 0.470860 5.2:+30595324 MS.gene55223:CDS
TTTAAGTCTGAAAACCGAAA+AGG 0.483010 5.2:-30595653 None:intergenic
CCCCTAGCAATAAAGAATCA+TGG 0.497001 5.2:+30595123 MS.gene55223:CDS
TTACAACCAACCCTTTCCTC+AGG 0.505082 5.2:-30595398 None:intergenic
AGCTGGAATATATTTGAAGC+TGG 0.507410 5.2:+30595421 MS.gene55223:CDS
ACAAAACAAGAAATCTGATA+AGG 0.511435 5.2:+30595253 MS.gene55223:CDS
CTAAGGTAACACGAATGCTC+TGG 0.517941 5.2:-30595509 None:intergenic
GTCAGCATGAATCATTGCTA+GGG 0.522802 5.2:-30595084 None:intergenic
GGTAATTATGCATATAACAC+TGG 0.523547 5.2:+30595191 MS.gene55223:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.525236 5.2:+30595298 MS.gene55223:CDS
TAAGATATATAGATGGGATG+TGG 0.528498 5.2:+30595463 MS.gene55223:CDS
GAATGCTCTGGTTTATGTAA+TGG 0.534744 5.2:-30595497 None:intergenic
CCATGATTCTTTATTGCTAG+GGG 0.537011 5.2:-30595123 None:intergenic
TGTCAGCATGAATCATTGCT+AGG 0.540740 5.2:-30595085 None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 5.2:+30595299 MS.gene55223:CDS
GTTATGGGTCAGGGAGTTGG+AGG 0.547221 5.2:+30595617 MS.gene55223:CDS
AACACAATAACGCACTAGCC+TGG 0.552106 5.2:-30596572 None:intergenic
GGCCTACCTCAGACTTTAGG+TGG 0.565384 5.2:+30596790 MS.gene55223:CDS
TGTGTATAAGATATATAGAT+GGG 0.601002 5.2:+30595457 MS.gene55223:CDS
TCACTGATTCATGCTGAGAG+TGG 0.606950 5.2:+30595044 MS.gene55223:CDS
TCTTATGAAGTTATGGGTCA+GGG 0.609153 5.2:+30595608 MS.gene55223:CDS
GCCAACAACAGACAGTCCAC+AGG 0.619933 5.2:+30595346 MS.gene55223:CDS
TTCACAAACTCAATAACCTG+TGG 0.626760 5.2:-30595362 None:intergenic
TGGTCTGTATAAATCGTCTG+TGG 0.650424 5.2:-30596552 None:intergenic
GACAAGCCACCTAAAGTCTG+AGG 0.653221 5.2:-30596796 None:intergenic
TCAGCATGAATCATTGCTAG+GGG 0.681156 5.2:-30595083 None:intergenic
AGCCACCTAAAGTCTGAGGT+AGG 0.684957 5.2:-30596792 None:intergenic
ACAGACGATTTATACAGACC+AGG 0.720235 5.2:+30596554 MS.gene55223:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGATGAGTTAAATAATT+TGG + chr5.2:30596695-30596714 MS.gene55223:CDS 15.0%
!! ACATACTTATTAGAATACTT+AGG + chr5.2:30596190-30596209 MS.gene55223:intron 20.0%
!! ATGTGTATAAGATATATAGA+TGG + chr5.2:30595456-30595475 MS.gene55223:CDS 20.0%
!! TGAATATCATAGTAAAAAGA+TGG - chr5.2:30595770-30595789 None:intergenic 20.0%
!! TGTGTATAAGATATATAGAT+GGG + chr5.2:30595457-30595476 MS.gene55223:CDS 20.0%
!!! AAAGAGATTGAAGATTTTTA+TGG + chr5.2:30595170-30595189 MS.gene55223:CDS 20.0%
!!! AATCAGATTTTGACTATAAT+TGG + chr5.2:30596736-30596755 MS.gene55223:CDS 20.0%
!!! ATCAGATTTTGACTATAATT+GGG + chr5.2:30596737-30596756 MS.gene55223:CDS 20.0%
!!! ATGATGATAAATTGTTTTGA+TGG + chr5.2:30596765-30596784 MS.gene55223:CDS 20.0%
!!! TTAATTTCATTAAGTTGTTC+TGG + chr5.2:30595960-30595979 MS.gene55223:intron 20.0%
!!! TTCTTATAATTTGTGATTAC+AGG + chr5.2:30596253-30596272 MS.gene55223:intron 20.0%
! ACAAAACAAGAAATCTGATA+AGG + chr5.2:30595253-30595272 MS.gene55223:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG + chr5.2:30595891-30595910 MS.gene55223:CDS 25.0%
! AGTAAAAAACACTAACCTAA+TGG - chr5.2:30595917-30595936 None:intergenic 25.0%
! ATGATTGTCAAAACCAATAA+AGG - chr5.2:30596430-30596449 None:intergenic 25.0%
! CAAACTTAAATGATGTTGAT+TGG + chr5.2:30596114-30596133 MS.gene55223:intron 25.0%
! CAACTAGATTATTCTCTATT+CGG + chr5.2:30595842-30595861 MS.gene55223:CDS 25.0%
! TCTTACTTATTTGAACTACA+CGG + chr5.2:30596036-30596055 MS.gene55223:intron 25.0%
!! ATAACACTGGATTTTCTATT+AGG + chr5.2:30595204-30595223 MS.gene55223:CDS 25.0%
!! TGTTGTATTTTGTTCTGATA+TGG + chr5.2:30596385-30596404 MS.gene55223:intron 25.0%
!! TTAACCATGTTGTTTGTTTA+GGG + chr5.2:30596061-30596080 MS.gene55223:intron 25.0%
!! TTTAACCATGTTGTTTGTTT+AGG + chr5.2:30596060-30596079 MS.gene55223:intron 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG + chr5.2:30595804-30595823 MS.gene55223:CDS 25.0%
!!! ATTTATATTTTGAACTGCCT+AGG + chr5.2:30596138-30596157 MS.gene55223:intron 25.0%
!!! TAGTGTTTTTTACTTCTTTC+AGG + chr5.2:30595923-30595942 MS.gene55223:intron 25.0%
!!! TGATAAATTGTTTTGATGGA+AGG + chr5.2:30596769-30596788 MS.gene55223:CDS 25.0%
AAAACACCTGGAAAACTATT+CGG - chr5.2:30596603-30596622 None:intergenic 30.0%
AAATCCCTAAACAAACAACA+TGG - chr5.2:30596068-30596087 None:intergenic 30.0%
AACAAAGAAGGTTTCAAGAA+GGG + chr5.2:30595299-30595318 MS.gene55223:CDS 30.0%
AACAATAAATATCGGCCATT+AGG + chr5.2:30595899-30595918 MS.gene55223:CDS 30.0%
AATAAGGTTTCTCTGTTAGT+TGG - chr5.2:30596821-30596840 None:intergenic 30.0%
AGAACGTCTTATGAAGTTAT+GGG + chr5.2:30595602-30595621 MS.gene55223:CDS 30.0%
CGTTATGTTTGTAACAAAGA+AGG + chr5.2:30595287-30595306 MS.gene55223:CDS 30.0%
CTTACTTATTTGAACTACAC+GGG + chr5.2:30596037-30596056 MS.gene55223:intron 30.0%
GAGAATGCAAAGAAAAATCT+TGG + chr5.2:30596651-30596670 MS.gene55223:intron 30.0%
GCCGATATTTATTGTTTGTT+CGG - chr5.2:30595895-30595914 None:intergenic 30.0%
GGTAATTATGCATATAACAC+TGG + chr5.2:30595191-30595210 MS.gene55223:CDS 30.0%
GTTTACTTAATCCTAACAAC+AGG + chr5.2:30595324-30595343 MS.gene55223:CDS 30.0%
TAAATTTCAGCTGCCTTTAT+TGG + chr5.2:30596414-30596433 MS.gene55223:intron 30.0%
TAACAAAGAAGGTTTCAAGA+AGG + chr5.2:30595298-30595317 MS.gene55223:CDS 30.0%
TAAGATATATAGATGGGATG+TGG + chr5.2:30595463-30595482 MS.gene55223:CDS 30.0%
TAAGTTTGTCTCAAACCAAA+TGG - chr5.2:30596102-30596121 None:intergenic 30.0%
TCCATGATTCTTTATTGCTA+GGG - chr5.2:30595127-30595146 None:intergenic 30.0%
TTACTTCTCGAAATGATCTA+AGG - chr5.2:30595529-30595548 None:intergenic 30.0%
TTCCATGATTCTTTATTGCT+AGG - chr5.2:30595128-30595147 None:intergenic 30.0%
TTTAAGTCTGAAAACCGAAA+AGG - chr5.2:30595656-30595675 None:intergenic 30.0%
!! GCTCTTATAAATTCTAAAGC+TGG + chr5.2:30595569-30595588 MS.gene55223:CDS 30.0%
AATCGTCTGTGGTTTCTTAT+TGG - chr5.2:30596544-30596563 None:intergenic 35.0%
AATTCGTCAAAAAGAGATGG+TGG + chr5.2:30595691-30595710 MS.gene55223:CDS 35.0%
ACATCAACTTCATCTGATAG+TGG + chr5.2:30594999-30595018 MS.gene55223:CDS 35.0%
AGTAAAAAGATGGTTTCGAG+AGG - chr5.2:30595760-30595779 None:intergenic 35.0%
CCATGATTCTTTATTGCTAG+GGG - chr5.2:30595126-30595145 None:intergenic 35.0%
CGAGAAGTAAATGAAGTTCA+AGG + chr5.2:30595539-30595558 MS.gene55223:CDS 35.0%
GAAAGAAAAACCTGAGGAAA+GGG + chr5.2:30595388-30595407 MS.gene55223:CDS 35.0%
GAAAGTGAAAGAAAAACCTG+AGG + chr5.2:30595382-30595401 MS.gene55223:CDS 35.0%
GACAATTCGTCAAAAAGAGA+TGG + chr5.2:30595688-30595707 MS.gene55223:CDS 35.0%
GAGAACGTCTTATGAAGTTA+TGG + chr5.2:30595601-30595620 MS.gene55223:CDS 35.0%
GTAAAAAGATGGTTTCGAGA+GGG - chr5.2:30595759-30595778 None:intergenic 35.0%
TAAGACCTATAGTCAATCCT+AGG - chr5.2:30596021-30596040 None:intergenic 35.0%
TCTTATGAAGTTATGGGTCA+GGG + chr5.2:30595608-30595627 MS.gene55223:CDS 35.0%
TGAAAGAAAAACCTGAGGAA+AGG + chr5.2:30595387-30595406 MS.gene55223:CDS 35.0%
TTCACAAACTCAATAACCTG+TGG - chr5.2:30595365-30595384 None:intergenic 35.0%
! AGCTGGAATATATTTGAAGC+TGG + chr5.2:30595421-30595440 MS.gene55223:CDS 35.0%
! AGTTTTCCAGGTGTTTTTCA+TGG + chr5.2:30596606-30596625 MS.gene55223:intron 35.0%
! ATTTAGTTTACGCATTGCTG+AGG + chr5.2:30596224-30596243 MS.gene55223:intron 35.0%
! GAATGCTCTGGTTTATGTAA+TGG - chr5.2:30595500-30595519 None:intergenic 35.0%
! GTTTTCCAGGTGTTTTTCAT+GGG + chr5.2:30596607-30596626 MS.gene55223:intron 35.0%
! TCAATTCCGAATAGTTTTCC+AGG + chr5.2:30596594-30596613 MS.gene55223:intron 35.0%
!!! CATCGAAAGAGTGTTTTGTT+TGG + chr5.2:30596447-30596466 MS.gene55223:CDS 35.0%
!!! GCTAGTATTTTTTGGTCAGA+TGG + chr5.2:30595812-30595831 MS.gene55223:CDS 35.0%
!!! TTTTTCATGGGCTTTGTTCA+TGG + chr5.2:30596619-30596638 MS.gene55223:intron 35.0%
AACTGCCTAGGATTGACTAT+AGG + chr5.2:30596013-30596032 MS.gene55223:intron 40.0%
ACAGACGATTTATACAGACC+AGG + chr5.2:30596554-30596573 MS.gene55223:CDS 40.0%
CCCCTAGCAATAAAGAATCA+TGG + chr5.2:30595123-30595142 MS.gene55223:CDS 40.0%
GTCAGCATGAATCATTGCTA+GGG - chr5.2:30595087-30595106 None:intergenic 40.0%
GTCTTATGAAGTTATGGGTC+AGG + chr5.2:30595607-30595626 MS.gene55223:CDS 40.0%
TCACACAAGACAAAATGCCT+AGG - chr5.2:30596158-30596177 None:intergenic 40.0%
TCAGCATGAATCATTGCTAG+GGG - chr5.2:30595086-30595105 None:intergenic 40.0%
TGGTCTGTATAAATCGTCTG+TGG - chr5.2:30596555-30596574 None:intergenic 40.0%
TGTCAGCATGAATCATTGCT+AGG - chr5.2:30595088-30595107 None:intergenic 40.0%
! ATTTTCTCGCTTCTGCCATT+TGG + chr5.2:30596084-30596103 MS.gene55223:intron 40.0%
! CAGTACATTTTGAACTGCCT+AGG + chr5.2:30596001-30596020 MS.gene55223:intron 40.0%
! GCACAGAGAATCTTCCTTTT+CGG + chr5.2:30595639-30595658 MS.gene55223:CDS 40.0%
!!! AGACAGCAGCTACTTTTGAT+TGG + chr5.2:30596490-30596509 MS.gene55223:CDS 40.0%
AACACAATAACGCACTAGCC+TGG - chr5.2:30596575-30596594 None:intergenic 45.0%
CAAAGCCCATGAAAAACACC+TGG - chr5.2:30596615-30596634 None:intergenic 45.0%
CTAAGGTAACACGAATGCTC+TGG - chr5.2:30595512-30595531 None:intergenic 45.0%
GAAAAACCTGAGGAAAGGGT+TGG + chr5.2:30595392-30595411 MS.gene55223:CDS 45.0%
TTACAACCAACCCTTTCCTC+AGG - chr5.2:30595401-30595420 None:intergenic 45.0%
! CGTCAAAAAGAGATGGTGGT+TGG + chr5.2:30595695-30595714 MS.gene55223:CDS 45.0%
! TCTGTTGTTGGCCTGTTGTT+AGG - chr5.2:30595338-30595357 None:intergenic 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG + chr5.2:30595404-30595423 MS.gene55223:CDS 45.0%
!! TCACTGATTCATGCTGAGAG+TGG + chr5.2:30595044-30595063 MS.gene55223:CDS 45.0%
GAAGGCCTACCTCAGACTTT+AGG + chr5.2:30596787-30596806 MS.gene55223:CDS 50.0%
GAAGTTATGGGTCAGGGAGT+TGG + chr5.2:30595614-30595633 MS.gene55223:CDS 50.0%
GACAAGCCACCTAAAGTCTG+AGG - chr5.2:30596799-30596818 None:intergenic 50.0%
! ACCTGTGGACTGTCTGTTGT+TGG - chr5.2:30595350-30595369 None:intergenic 50.0%
! AGCCACCTAAAGTCTGAGGT+AGG - chr5.2:30596795-30596814 None:intergenic 50.0%
!! TGGGCTTTGTTCATGGCACA+TGG + chr5.2:30596626-30596645 MS.gene55223:intron 50.0%
GCCAACAACAGACAGTCCAC+AGG + chr5.2:30595346-30595365 MS.gene55223:CDS 55.0%
GGCCTACCTCAGACTTTAGG+TGG + chr5.2:30596790-30596809 MS.gene55223:CDS 55.0%
GTTATGGGTCAGGGAGTTGG+AGG + chr5.2:30595617-30595636 MS.gene55223:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 30594990 30596842 30594990 ID=MS.gene55223
chr5.2 mRNA 30594990 30596842 30594990 ID=MS.gene55223.t1;Parent=MS.gene55223
chr5.2 exon 30594990 30595920 30594990 ID=MS.gene55223.t1.exon1;Parent=MS.gene55223.t1
chr5.2 CDS 30594990 30595920 30594990 ID=cds.MS.gene55223.t1;Parent=MS.gene55223.t1
chr5.2 exon 30596424 30596579 30596424 ID=MS.gene55223.t1.exon2;Parent=MS.gene55223.t1
chr5.2 CDS 30596424 30596579 30596424 ID=cds.MS.gene55223.t1;Parent=MS.gene55223.t1
chr5.2 exon 30596652 30596842 30596652 ID=MS.gene55223.t1.exon3;Parent=MS.gene55223.t1
chr5.2 CDS 30596652 30596842 30596652 ID=cds.MS.gene55223.t1;Parent=MS.gene55223.t1
Gene Sequence

>MS.gene55223

ATGGATTTGACATCAACTTCATCTGATAGTGGACAACTGAGTGTTGAAACTTCATCACTGATTCATGCTGAGAGTGGACAATTGAGTGTTACTCCCCTAGCAATGATTCATGCTGACAGTTCTCAATGTTACTCCCCTAGCAATAAAGAATCATGGAAGTCTTTAGTCTTTCAATCTATTAAAGAGATTGAAGATTTTTATGGTAATTATGCATATAACACTGGATTTTCTATTAGGACTATGTTGAAGTCGAAGAATAATTCACAAAACAAGAAATCTGATAAGGTACATTATTTGCGTTATGTTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTTTACTTAATCCTAACAACAGGCCAACAACAGACAGTCCACAGGTTATTGAGTTTGTGAAAGTGAAAGAAAAACCTGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGCTGGATGAAGTTTTGAATGTGTATAAGATATATAGATGGGATGTGGATCACTGTCATCCATTACATAAACCAGAGCATTCGTGTTACCTTAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTAGCTCTTATAAATTCTAAAGCTGGAATGTCGATGAGAACGTCTTATGAAGTTATGGGTCAGGGAGTTGGAGGCACAGAGAATCTTCCTTTTCGGTTTTCAGACTTAAAGAACTATCTAATGACAATTCGTCAAAAAGAGATGGTGGTTGGTGAAGCAACTGTAATTCAAGAATTCTTTAGAAATGAAGCCCTCTCGAAACCATCTTTTTACTATGATATTCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGTCAGATGGAATTATGCAACTAGATTATTCTCTATTCGGCGATGTCATCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGGTTAGTGTTTTTTACTTCTTTCAGGTTTTAAATTATTGTTTAATTTCATTAAGTTGTTCTGGCATGTGTATTATAAGTAGCAGTACATTTTGAACTGCCTAGGATTGACTATAGGTCTTACTTATTTGAACTACACGGGTTTAACCATGTTGTTTGTTTAGGGATTTTCTCGCTTCTGCCATTTGGTTTGAGACAAACTTAAATGATGTTGATTGGAATTTATATTTTGAACTGCCTAGGCATTTTGTCTTGTGTGATCGAACTTTAGTACATACTTATTAGAATACTTAGGTGTGTTTTATCATTTAGTTTACGCATTGCTGAGGCATGATTTCTTATAATTTGTGATTACAGGCTAGCAATGACTTAAATTAGTGATTTCTCTAATTATTTACTGTAAGCTATATATATATATATATATATATATATATATATATATATATATATATATGTGTGTGTGTGTGTGTTGTATTTTGTTCTGATATGGATAATTTAAATTTCAGCTGCCTTTATTGGTTTTGACAATCATCGAAAGAGTGTTTTGTTTGGTGCTGCGCTATTGTACGATGAGACAGCAGCTACTTTTGATTGGTTGTTTAAAACATTCTTGAAGTGTATGTCCAATAAGAAACCACAGACGATTTATACAGACCAGGCTAGTGCGTTATTGTGTTCAATTCCGAATAGTTTTCCAGGTGTTTTTCATGGGCTTTGTTCATGGCACATGGCAGAGAATGCAAAGAAAAATCTTGGCTCTCGTGCAAACAGTGCATTTTTTGATGAGTTAAATAATTTGGTTTCAAATGTAGATGATGAATCAGATTTTGACTATAATTGGGATCAGATGATGATAAATTGTTTTGATGGAAGGCCTACCTCAGACTTTAGGTGGCTTGTCCAACTAACAGAGAAACCTTATTGA
Protein sequence

>MS.gene55223.t1

MDLTSTSSDSGQLSVETSSLIHAESGQLSVTPLAMIHADSSQCYSPSNKESWKSLVFQSIKEIEDFYGNYAYNTGFSIRTMLKSKNNSQNKKSDKVHYLRYVCNKEGFKKGSLLNPNNRPTTDSPQVIEFVKVKEKPEERVGCKAGIYLKLDEVLNVYKIYRWDVDHCHPLHKPEHSCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTIRQKEMVVGEATVIQEFFRNEALSKPSFYYDIQVDAAEDIASIFWSDGIMQLDYSLFGDVISFDTTYRTNNKYRPLAAFIGFDNHRKSVLFGAALLYDETAATFDWLFKTFLKCMSNKKPQTIYTDQAKNAKKNLGSRANSAFFDELNNLVSNVDDESDFDYNWDQMMINCFDGRPTSDFRWLVQLTEKPY