AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55225

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55225.t1 MTR_5g040740 78.959 461 45 4 1 460 1 410 0.0 707
MS.gene55225.t1 MTR_5g040880 63.740 524 113 9 1 456 1 515 0.0 617
MS.gene55225.t1 MTR_5g082140 61.325 468 153 7 1 450 1 458 0.0 544
MS.gene55225.t1 MTR_2g024430 64.833 418 135 7 1 415 9 417 0.0 516
MS.gene55225.t1 MTR_5g061220 50.000 536 172 13 1 456 8 527 5.00e-155 450
MS.gene55225.t1 MTR_2g006270 47.280 478 193 12 4 454 5 450 2.84e-129 382
MS.gene55225.t1 MTR_1g058210 48.172 465 201 15 1 448 1 442 2.13e-127 377
MS.gene55225.t1 MTR_2g006380 44.632 475 199 14 6 454 14 450 3.60e-116 348
MS.gene55225.t1 MTR_3g073420 42.857 455 211 10 2 415 9 455 2.25e-107 330
MS.gene55225.t1 MTR_5g060780 48.942 378 158 11 1 362 1 359 7.77e-104 315
MS.gene55225.t1 MTR_5g460920 48.942 378 158 11 1 362 1 359 5.74e-103 313
MS.gene55225.t1 MTR_5g061890 48.379 401 161 13 1 385 1 371 8.93e-101 307
MS.gene55225.t1 MTR_5g060630 43.621 486 188 16 1 411 1 475 1.67e-97 303
MS.gene55225.t1 MTR_5g460740 43.621 486 188 16 1 411 1 475 1.67e-97 303
MS.gene55225.t1 MTR_2g094570 38.865 458 198 13 9 405 8 444 2.35e-92 293
MS.gene55225.t1 MTR_2g094570 38.865 458 198 13 9 405 8 444 3.63e-92 294
MS.gene55225.t1 MTR_2g094570 38.865 458 198 13 9 405 64 500 1.21e-91 294
MS.gene55225.t1 MTR_1g064430 37.691 459 207 10 9 405 24 465 3.21e-88 283
MS.gene55225.t1 MTR_1g064430 37.691 459 207 10 9 405 37 478 3.67e-88 283
MS.gene55225.t1 MTR_4g058930 37.815 476 203 13 1 408 1 451 2.81e-87 280
MS.gene55225.t1 MTR_1g094960 38.528 462 203 13 9 408 16 458 4.52e-87 278
MS.gene55225.t1 MTR_4g058930 37.815 476 203 13 1 408 1 451 5.07e-87 280
MS.gene55225.t1 MTR_7g101280 34.839 465 221 14 9 408 28 475 5.75e-76 250
MS.gene55225.t1 MTR_0041s0110 34.839 465 221 14 9 408 28 475 5.75e-76 250
MS.gene55225.t1 MTR_5g062970 55.051 198 83 2 9 203 35 229 1.27e-65 211
MS.gene55225.t1 MTR_7g101275 38.941 321 157 9 23 321 3 306 3.19e-65 215
MS.gene55225.t1 MTR_0041s0120 36.890 328 166 8 23 321 3 318 9.15e-60 201
MS.gene55225.t1 MTR_2g093740 35.075 402 223 15 12 385 56 447 3.66e-55 195
MS.gene55225.t1 MTR_2g093740 35.075 402 223 15 12 385 56 447 5.85e-55 195
MS.gene55225.t1 MTR_2g093740 35.075 402 223 15 12 385 56 447 1.28e-54 195
MS.gene55225.t1 MTR_4g021580 32.249 369 214 8 10 356 17 371 1.29e-53 190
MS.gene55225.t1 MTR_4g021580 32.249 369 214 8 10 356 17 371 1.45e-53 190
MS.gene55225.t1 MTR_4g088210 33.149 362 183 11 12 337 38 376 1.80e-53 191
MS.gene55225.t1 MTR_4g088210 33.149 362 183 11 12 337 38 376 1.81e-53 191
MS.gene55225.t1 MTR_2g014770 33.051 354 190 11 12 337 53 387 2.28e-53 189
MS.gene55225.t1 MTR_2g014770 33.051 354 190 11 12 337 53 387 5.61e-53 189
MS.gene55225.t1 MTR_8g100050 33.333 360 218 9 12 356 59 411 4.89e-52 187
MS.gene55225.t1 MTR_2g093740 33.976 415 223 16 12 385 56 460 9.93e-52 186
MS.gene55225.t1 MTR_8g100050 33.333 360 218 9 12 356 59 411 1.43e-51 187
MS.gene55225.t1 MTR_5g060770 38.187 364 156 13 61 411 7 314 1.44e-51 178
MS.gene55225.t1 MTR_2g005240 35.262 363 206 10 12 356 23 374 1.53e-51 185
MS.gene55225.t1 MTR_2g093740 33.976 415 223 16 12 385 56 460 2.04e-51 186
MS.gene55225.t1 MTR_7g062540 31.166 446 249 16 12 425 14 433 2.29e-51 184
MS.gene55225.t1 MTR_2g093740 33.976 415 223 16 12 385 56 460 3.20e-51 186
MS.gene55225.t1 MTR_2g018690 32.718 379 204 14 9 359 20 375 3.35e-51 186
MS.gene55225.t1 MTR_8g079492 32.190 379 205 13 9 359 20 374 4.64e-50 183
MS.gene55225.t1 MTR_8g079492 32.190 379 205 13 9 359 20 374 4.98e-50 183
MS.gene55225.t1 MTR_5g076270 31.675 382 204 13 9 359 21 376 7.80e-49 179
MS.gene55225.t1 MTR_8g101360 32.507 363 192 12 9 340 28 368 1.02e-48 179
MS.gene55225.t1 MTR_3g064050 31.675 382 204 13 9 359 23 378 1.98e-48 178
MS.gene55225.t1 MTR_8g027440 34.302 344 194 11 12 337 29 358 2.63e-48 176
MS.gene55225.t1 MTR_4g124900 31.694 366 212 11 9 351 20 370 1.08e-47 176
MS.gene55225.t1 MTR_4g124900 31.694 366 212 11 9 351 20 370 1.28e-47 176
MS.gene55225.t1 MTR_4g060460 34.783 368 212 11 12 356 54 416 1.64e-47 175
MS.gene55225.t1 MTR_4g060460 34.783 368 212 11 12 356 54 416 1.80e-47 175
MS.gene55225.t1 MTR_2g043250 32.022 356 199 11 9 339 20 357 2.24e-47 176
MS.gene55225.t1 MTR_1g024025 31.624 351 204 10 9 337 40 376 2.83e-47 175
MS.gene55225.t1 MTR_2g093740 36.842 285 160 10 114 385 40 317 2.47e-40 153
MS.gene55225.t1 MTR_8g079492 32.899 307 170 11 72 359 3 292 3.21e-40 154
MS.gene55225.t1 MTR_2g093740 36.842 285 160 10 114 385 40 317 5.30e-40 152
MS.gene55225.t1 MTR_2g093740 35.235 298 160 11 114 385 40 330 5.15e-37 144
MS.gene55225.t1 MTR_2g093740 35.235 298 160 11 114 385 40 330 8.18e-37 144
MS.gene55225.t1 MTR_5g062960 43.915 189 71 6 235 415 2 163 4.76e-35 129
MS.gene55225.t1 MTR_5g460910 33.245 376 157 16 51 411 13 309 5.08e-32 125
MS.gene55225.t1 MTR_8g446900 28.280 343 205 11 61 379 12 337 3.44e-31 124
MS.gene55225.t1 MTR_5g046120 41.228 114 58 4 53 161 15 124 3.34e-15 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55225.t1 AT1G77850 40.135 446 209 12 9 408 16 449 1.90e-101 315
MS.gene55225.t1 AT1G77850 40.135 446 209 12 9 408 16 449 4.17e-101 314
MS.gene55225.t1 AT4G30080 38.852 453 205 10 9 406 16 451 2.01e-89 286
MS.gene55225.t1 AT2G28350 40.952 420 167 13 4 359 2 404 9.07e-87 279
MS.gene55225.t1 AT2G28350 40.952 420 167 13 4 359 2 404 1.41e-86 279
MS.gene55225.t1 AT2G33860 35.328 351 186 10 12 337 54 388 2.51e-56 197
MS.gene55225.t1 AT4G23980 33.621 348 200 9 12 341 12 346 7.70e-55 193
MS.gene55225.t1 AT4G23980 33.621 348 200 9 12 341 12 346 7.85e-55 193
MS.gene55225.t1 AT1G35520 35.792 366 194 13 12 356 27 372 3.45e-52 185
MS.gene55225.t1 AT5G37020 31.903 373 215 12 9 359 19 374 1.45e-51 186
MS.gene55225.t1 AT5G37020 31.903 373 215 12 9 359 19 374 2.00e-51 186
MS.gene55225.t1 AT5G60450 36.441 354 190 12 9 337 38 381 2.07e-51 186
MS.gene55225.t1 AT5G60450 36.441 354 190 12 9 337 62 405 2.50e-51 186
MS.gene55225.t1 AT2G46530 34.571 350 191 12 12 340 21 353 2.84e-51 183
MS.gene55225.t1 AT2G46530 34.571 350 191 12 12 340 42 374 5.10e-51 182
MS.gene55225.t1 AT3G61830 33.048 351 199 10 7 337 20 354 4.46e-50 180
MS.gene55225.t1 AT1G59750 32.329 365 211 10 12 354 22 372 4.57e-50 181
MS.gene55225.t1 AT1G59750 32.329 365 211 10 12 354 22 372 5.23e-50 181
MS.gene55225.t1 AT1G59750 32.329 365 211 10 12 354 22 372 5.45e-50 181
MS.gene55225.t1 AT1G59750 32.329 365 211 10 12 354 22 372 5.45e-50 181
MS.gene55225.t1 AT5G62000 33.060 366 211 10 12 356 61 413 5.98e-50 182
MS.gene55225.t1 AT5G62000 33.060 366 211 10 12 356 61 413 5.98e-50 182
MS.gene55225.t1 AT5G62000 33.060 366 211 10 12 356 61 413 5.98e-50 182
MS.gene55225.t1 AT5G62000 33.060 366 211 10 12 356 61 413 5.98e-50 182
MS.gene55225.t1 AT5G62000 33.060 366 211 10 12 356 61 413 6.84e-50 182
MS.gene55225.t1 AT1G35240 33.880 366 201 12 12 356 27 372 3.89e-49 177
MS.gene55225.t1 AT1G30330 31.467 375 214 11 9 359 20 375 6.34e-49 180
MS.gene55225.t1 AT1G30330 31.467 375 214 11 9 359 22 377 6.89e-49 180
MS.gene55225.t1 AT1G30330 31.467 375 214 11 9 359 22 377 6.89e-49 180
MS.gene55225.t1 AT1G34410 34.153 366 200 13 12 356 27 372 1.80e-48 176
MS.gene55225.t1 AT1G19220 31.842 380 203 13 9 356 20 375 2.27e-48 178
MS.gene55225.t1 AT1G19850 33.333 348 203 11 9 337 51 388 3.81e-48 177
MS.gene55225.t1 AT1G19850 33.333 348 203 11 9 337 40 377 4.49e-48 177
MS.gene55225.t1 AT1G34390 33.333 366 201 13 12 356 27 370 1.89e-47 172
MS.gene55225.t1 AT1G34310 34.060 367 199 13 12 356 27 372 2.80e-47 172
MS.gene55225.t1 AT5G20730 31.536 371 211 11 9 353 21 374 4.51e-46 172
MS.gene55225.t1 AT1G35540 34.393 346 187 12 12 337 27 352 4.85e-46 169
MS.gene55225.t1 AT5G20730 31.536 371 211 11 9 353 21 374 5.25e-46 172
MS.gene55225.t1 AT5G20730 31.806 371 209 12 9 353 21 373 1.39e-45 170
MS.gene55225.t1 AT1G34170 32.665 349 191 12 12 337 25 352 8.09e-43 158
MS.gene55225.t1 AT2G33860 36.820 239 125 7 114 337 4 231 2.06e-41 153
MS.gene55225.t1 AT3G61830 34.122 296 162 9 59 337 24 303 9.29e-40 150
MS.gene55225.t1 AT5G37020 33.721 258 148 8 114 359 33 279 7.40e-39 150
MS.gene55225.t1 AT2G46530 36.777 242 124 9 114 340 39 266 4.55e-36 140
MS.gene55225.t1 AT1G34170 31.034 348 172 12 12 337 25 326 5.42e-36 139
MS.gene55225.t1 AT1G34170 31.034 348 172 12 12 337 25 326 1.68e-35 138
MS.gene55225.t1 AT1G43950 35.514 214 113 6 12 218 27 222 3.35e-30 117

Find 113 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCAATATCATAGGTTAAT+TGG 0.114277 5.2:+30626112 MS.gene55225:intron
CCTGGTTCAATTATGTTATT+TGG 0.201738 5.2:+30626447 MS.gene55225:CDS
GGGAGAGTGTGCTAGGGTTT+GGG 0.238185 5.2:-30624758 None:intergenic
CATGTTTACTACTTCCCATT+AGG 0.255048 5.2:+30624711 MS.gene55225:CDS
GAAGAATGAAACTCTATCTT+TGG 0.260394 5.2:-30626300 None:intergenic
GGAAGTGATTTACACGATTC+AGG 0.271828 5.2:+30626489 MS.gene55225:CDS
GGAACCAGCTGCGAAACTTT+AGG 0.293485 5.2:-30626141 None:intergenic
AGGGAGAGTGTGCTAGGGTT+TGG 0.295398 5.2:-30624759 None:intergenic
AGAATTCCATTTAATCTTCA+TGG 0.301340 5.2:-30625432 None:intergenic
ATGTGTTCTAAGTGCCCTAA+TGG 0.313804 5.2:-30624726 None:intergenic
TAAACATGAGAATGTAGTTT+TGG 0.329887 5.2:-30624696 None:intergenic
ATGAAGATTAAATGGAATTC+TGG 0.341022 5.2:+30625434 MS.gene55225:CDS
GGTCGTTAGTGACGGGAGTT+AGG 0.352092 5.2:-30624875 None:intergenic
GGGGTTGAGGTTGTTTCCTC+TGG 0.358289 5.2:+30624939 MS.gene55225:CDS
ATGTTTACTACTTCCCATTA+GGG 0.360944 5.2:+30624712 MS.gene55225:CDS
AGGATGCCATGAAGATTAAA+TGG 0.366991 5.2:+30625426 MS.gene55225:CDS
GTCAATATCATAGGTTAATT+GGG 0.372441 5.2:+30626113 MS.gene55225:intron
GGAATTGGCGGAGAAGAATA+TGG 0.390857 5.2:+30625334 MS.gene55225:CDS
GGAATCCGCCGGCAGAAGTT+TGG 0.393817 5.2:+30625269 MS.gene55225:CDS
TGTTACCGAGGCTGTGGAAT+TGG 0.396051 5.2:+30625319 MS.gene55225:CDS
AAGAATGAAACTCTATCTTT+GGG 0.410608 5.2:-30626299 None:intergenic
ATTCAACGATGTTTCATTGA+AGG 0.425033 5.2:-30626334 None:intergenic
AAAATCTGGCAGTGCTGCGC+CGG 0.426459 5.2:+30624657 MS.gene55225:CDS
CACTCCAGTTAGTGGTGATC+AGG 0.431295 5.2:-30625148 None:intergenic
TTCCAGAGCGAGTCAGTCCT+TGG 0.433985 5.2:+30626160 MS.gene55225:CDS
AGCATGCGCCATGGACGATT+AGG 0.436142 5.2:-30625542 None:intergenic
GACACCTGATCACCACTAAC+TGG 0.440049 5.2:+30625144 MS.gene55225:CDS
TGCTTTGCTCGATGACATGC+AGG 0.446126 5.2:+30626362 MS.gene55225:CDS
GCAGTTGGAAGAAGTTGATT+CGG 0.450852 5.2:-30626406 None:intergenic
TGCCTTACACAATGATATAG+AGG 0.453622 5.2:+30626263 MS.gene55225:CDS
TCCCAAGGACTGACTCGCTC+TGG 0.456814 5.2:-30626162 None:intergenic
TTCGTGAAGACGAAGATGAC+GGG 0.459706 5.2:+30624919 MS.gene55225:CDS
TGTAGTTAATGCTAAGGTTG+TGG 0.462109 5.2:+30625403 MS.gene55225:CDS
AACAAACTCACTCCAGTTAG+TGG 0.468954 5.2:-30625156 None:intergenic
TCGATGACATGCAGGGAGCC+AGG 0.473164 5.2:+30626370 MS.gene55225:CDS
AGGACATTCACGGCAAAGAA+TGG 0.478604 5.2:+30625087 MS.gene55225:CDS
TGGAGAAGGGAGAGTGTGCT+AGG 0.478757 5.2:-30624765 None:intergenic
GGAGAGTGTGCTAGGGTTTG+GGG 0.481560 5.2:-30624757 None:intergenic
ATCAACCGCGGAGATGGTGC+AGG 0.489672 5.2:-30624808 None:intergenic
TCTTCGTCTTCACGAACCTC+AGG 0.495857 5.2:-30624912 None:intergenic
GAGATGGTGCAGGGAATGAA+TGG 0.500260 5.2:-30624798 None:intergenic
TTTACACGATTCAGGTATCA+AGG 0.500760 5.2:+30626497 MS.gene55225:CDS
TGTGTTCTAAGTGCCCTAAT+GGG 0.500812 5.2:-30624725 None:intergenic
TCCAGAGCGAGTCAGTCCTT+GGG 0.507376 5.2:+30626161 MS.gene55225:CDS
AAATCTGGCAGTGCTGCGCC+GGG 0.508255 5.2:+30624658 MS.gene55225:CDS
TCGTGTAAATCACTTCCAAC+AGG 0.511200 5.2:-30626483 None:intergenic
TCCCTCTATATCATTGTGTA+AGG 0.511256 5.2:-30626265 None:intergenic
TACCGAGGCTGTGGAATTGG+CGG 0.515488 5.2:+30625322 MS.gene55225:CDS
AAAATCTGTTACCGAGGCTG+TGG 0.517941 5.2:+30625313 MS.gene55225:CDS
GAGTAGATCAACCGCGGAGA+TGG 0.518018 5.2:-30624814 None:intergenic
TGAACCTAAAGTTTCGCAGC+TGG 0.518797 5.2:+30626137 MS.gene55225:CDS
AGCACACTCTCCCTTCTCCA+TGG 0.523233 5.2:+30624768 MS.gene55225:CDS
AGACGAAGATGACGGGGTTG+AGG 0.523787 5.2:+30624926 MS.gene55225:CDS
CAACTTGGAGGTCAAGTGGC+GGG 0.528362 5.2:-30625037 None:intergenic
CCTCAGGAGGTTCGTGGACA+CGG 0.532546 5.2:-30624896 None:intergenic
TGGCAGCACCAAACTTCTGC+CGG 0.534995 5.2:-30625277 None:intergenic
CACGAACCTCAGGAGGTTCG+TGG 0.537961 5.2:-30624902 None:intergenic
GATTCCAACGGAGATCGTTA+CGG 0.541776 5.2:-30625252 None:intergenic
CTCCGCCAATTCCACAGCCT+CGG 0.546075 5.2:-30625324 None:intergenic
GTCTCAAGAACTCTCAGTAA+AGG 0.546981 5.2:+30625067 MS.gene55225:CDS
GTTGACGAGAAGAAACTGAT+TGG 0.549713 5.2:+30625176 MS.gene55225:CDS
TGGACGAGAACCATGGAGAA+GGG 0.549731 5.2:-30624778 None:intergenic
TCGTCCATGGCGCATGCTTG+AGG 0.553392 5.2:+30625547 MS.gene55225:CDS
AGCTTCGCCACGTCTACCGT+GGG 0.564056 5.2:+30625111 MS.gene55225:CDS
ATGAAACTCTATCTTTGGGA+TGG 0.564911 5.2:-30626295 None:intergenic
GTGGACACGGTCGTTAGTGA+CGG 0.566445 5.2:-30624883 None:intergenic
AGTGTGCTAGGGTTTGGGGA+TGG 0.567118 5.2:-30624753 None:intergenic
AAACTCTATCTTTGGGATGG+AGG 0.569440 5.2:-30626292 None:intergenic
AGTGGTGATCAGGTGTCGCA+AGG 0.570426 5.2:-30625138 None:intergenic
GTGTATTATCCTACTGCTGA+AGG 0.576265 5.2:+30625368 MS.gene55225:CDS
TATCTGTTCCTAATCGTCCA+TGG 0.580012 5.2:+30625534 MS.gene55225:CDS
CGATCTCCGTTGGAATCCGC+CGG 0.580469 5.2:+30625258 MS.gene55225:CDS
TCACACCAACCTTCAGCAGT+AGG 0.580736 5.2:-30625377 None:intergenic
ATGGACGAGAACCATGGAGA+AGG 0.582820 5.2:-30624779 None:intergenic
GTTAGGTTGCTGAGGAGACA+TGG 0.585884 5.2:-30624622 None:intergenic
GAAATAAGCTCAACCTCCCA+AGG 0.586576 5.2:-30626177 None:intergenic
CTCTCAGTAAAGGACATTCA+CGG 0.586911 5.2:+30625077 MS.gene55225:CDS
GGTCAGATCATACGTCCTGT+TGG 0.588139 5.2:+30626468 MS.gene55225:CDS
ATTATCCTACTGCTGAAGGT+TGG 0.588623 5.2:+30625372 MS.gene55225:CDS
TGGACACGGTCGTTAGTGAC+GGG 0.588676 5.2:-30624882 None:intergenic
TCGTCTTCACGAACCTCAGG+AGG 0.589597 5.2:-30624909 None:intergenic
CCAAATAACATAATTGAACC+AGG 0.590972 5.2:-30626447 None:intergenic
TTACACGATTCAGGTATCAA+GGG 0.593091 5.2:+30626498 MS.gene55225:CDS
ACTCCGTCTGATGCTAATAA+CGG 0.598328 5.2:+30624972 MS.gene55225:CDS
TGGATGTAGTGCATGTATGT+TGG 0.599102 5.2:-30626199 None:intergenic
GTTCGTGAAGACGAAGATGA+CGG 0.606081 5.2:+30624918 MS.gene55225:CDS
GTGGTGATCAGGTGTCGCAA+GGG 0.611088 5.2:-30625137 None:intergenic
TACACGATTCAGGTATCAAG+GGG 0.611714 5.2:+30626499 MS.gene55225:CDS
GCCTTACACAATGATATAGA+GGG 0.612818 5.2:+30626264 MS.gene55225:CDS
ACTGCCGTAACGATCTCCGT+TGG 0.613324 5.2:+30625248 MS.gene55225:CDS
GAATGAATGGACGAGAACCA+TGG 0.626500 5.2:-30624785 None:intergenic
AGGGAGTCCCACGGTAGACG+TGG 0.627277 5.2:-30625118 None:intergenic
CTTCTGCCGGCGGATTCCAA+CGG 0.627932 5.2:-30625264 None:intergenic
AGAGCGAGTCAGTCCTTGGG+AGG 0.629409 5.2:+30626164 MS.gene55225:CDS
TATACCTCAAGCATGCGCCA+TGG 0.631418 5.2:-30625551 None:intergenic
CAGCACCAAACTTCTGCCGG+CGG 0.635134 5.2:-30625274 None:intergenic
TTTACTGAGAGTTCTTGAGA+CGG 0.640719 5.2:-30625065 None:intergenic
TCAATACGGTTAGGTTGCTG+AGG 0.643017 5.2:-30624630 None:intergenic
AAGCTTCGCCACGTCTACCG+TGG 0.643601 5.2:+30625110 MS.gene55225:CDS
CCGTGTCCACGAACCTCCTG+AGG 0.646741 5.2:+30624896 MS.gene55225:CDS
GGAGAAGGGAGAGTGTGCTA+GGG 0.651561 5.2:-30624764 None:intergenic
TCAACTTGGAGGTCAAGTGG+CGG 0.652917 5.2:-30625038 None:intergenic
TCAACCGCGGAGATGGTGCA+GGG 0.652975 5.2:-30624807 None:intergenic
GACGAGAAGAAACTGATTGG+TGG 0.658536 5.2:+30625179 MS.gene55225:CDS
GCTTTGCTCGATGACATGCA+GGG 0.669755 5.2:+30626363 MS.gene55225:CDS
AGTTAATGCTAAGGTTGTGG+AGG 0.669949 5.2:+30625406 MS.gene55225:CDS
CCACCGTTATTAGCATCAGA+CGG 0.670947 5.2:-30624975 None:intergenic
GTCAGCGAGTAGATCAACCG+CGG 0.673456 5.2:-30624820 None:intergenic
CATTCCCTGCACCATCTCCG+CGG 0.674145 5.2:+30624803 MS.gene55225:CDS
GGTGTCGCAAGGGAGTCCCA+CGG 0.699974 5.2:-30625127 None:intergenic
TCGTGAAGACGAAGATGACG+GGG 0.700053 5.2:+30624920 MS.gene55225:CDS
CCGTCTGATGCTAATAACGG+TGG 0.711385 5.2:+30624975 MS.gene55225:CDS
ACACGATTCAGGTATCAAGG+GGG 0.772617 5.2:+30626500 MS.gene55225:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGTTTAATAAATGTTAT+CGG + chr5.2:30625639-30625658 MS.gene55225:intron 10.0%
!! ATTTGTACAATATACTTTAT+TGG + chr5.2:30625982-30626001 MS.gene55225:intron 15.0%
!!! ACATCTCATTTTATTTTTTA+AGG - chr5.2:30626057-30626076 None:intergenic 15.0%
!! AGAAACTTTATACTTGAAAT+AGG + chr5.2:30625889-30625908 MS.gene55225:intron 20.0%
!! CAAAATTCGCTATATTTATA+GGG - chr5.2:30626026-30626045 None:intergenic 20.0%
!! TCAAAATTCGCTATATTTAT+AGG - chr5.2:30626027-30626046 None:intergenic 20.0%
!!! AATGTTTAAGATTAACACAT+TGG - chr5.2:30625756-30625775 None:intergenic 20.0%
!!! ATATCCTATATTTTTCACAT+TGG - chr5.2:30625730-30625749 None:intergenic 20.0%
!!! ATGTAGTTTTGGAATTTTAA+AGG - chr5.2:30624688-30624707 None:intergenic 20.0%
!!! ATTTTTCATGTCAATATCAT+AGG + chr5.2:30626104-30626123 MS.gene55225:intron 20.0%
!!! GATGTTTTAAATGATTGATA+CGG + chr5.2:30626072-30626091 MS.gene55225:intron 20.0%
!!! TGATTTTTTCATGAAAACTA+AGG - chr5.2:30625210-30625229 None:intergenic 20.0%
!!! TTTTTCTTTTTTTGAGCAAA+TGG + chr5.2:30625923-30625942 MS.gene55225:intron 20.0%
! AAGAATGAAACTCTATCTTT+GGG - chr5.2:30626302-30626321 None:intergenic 25.0%
! AATGTTATCGGTCAAATTTA+TGG + chr5.2:30625651-30625670 MS.gene55225:intron 25.0%
! AGAATTCCATTTAATCTTCA+TGG - chr5.2:30625435-30625454 None:intergenic 25.0%
! ATGAAGATTAAATGGAATTC+TGG + chr5.2:30625434-30625453 MS.gene55225:CDS 25.0%
! ATTGTAGTTAATTCATGATG+TGG - chr5.2:30625835-30625854 None:intergenic 25.0%
! GTCAATATCATAGGTTAATT+GGG + chr5.2:30626113-30626132 MS.gene55225:intron 25.0%
! GTTGTGCAATTAAATTTAAG+TGG + chr5.2:30625779-30625798 MS.gene55225:intron 25.0%
! TACGATATGACTCATAAAAA+AGG - chr5.2:30625812-30625831 None:intergenic 25.0%
! TCAAATTTATGGTTCATGTT+AGG + chr5.2:30625662-30625681 MS.gene55225:intron 25.0%
! TGTCAATATCATAGGTTAAT+TGG + chr5.2:30626112-30626131 MS.gene55225:intron 25.0%
! TGTTCCAATGTGAAAAATAT+AGG + chr5.2:30625723-30625742 MS.gene55225:intron 25.0%
!! AGATTTTTCTGTTATCTTAG+TGG - chr5.2:30625300-30625319 None:intergenic 25.0%
!! TAAACATGAGAATGTAGTTT+TGG - chr5.2:30624699-30624718 None:intergenic 25.0%
!! TGATTTTGTAGTTAATGCTA+AGG + chr5.2:30625397-30625416 MS.gene55225:CDS 25.0%
!!! AGTCATTGTAGTTTTTTTTG+AGG - chr5.2:30625867-30625886 None:intergenic 25.0%
!!! TTTTTTGATCAGTTCATGTT+AGG + chr5.2:30625693-30625712 MS.gene55225:intron 25.0%
ATGTTTACTACTTCCCATTA+GGG + chr5.2:30624712-30624731 MS.gene55225:CDS 30.0%
ATTCAACGATGTTTCATTGA+AGG - chr5.2:30626337-30626356 None:intergenic 30.0%
CCAAATAACATAATTGAACC+AGG - chr5.2:30626450-30626469 None:intergenic 30.0%
CCTGGTTCAATTATGTTATT+TGG + chr5.2:30626447-30626466 MS.gene55225:CDS 30.0%
GAAGAATGAAACTCTATCTT+TGG - chr5.2:30626303-30626322 None:intergenic 30.0%
! AAAGAGATGCTCAAATTTTG+AGG + chr5.2:30625496-30625515 MS.gene55225:CDS 30.0%
! AAGAGATGCTCAAATTTTGA+GGG + chr5.2:30625497-30625516 MS.gene55225:CDS 30.0%
! GTTTTCATGAAAAAATCAGC+TGG + chr5.2:30625212-30625231 MS.gene55225:CDS 30.0%
! TATTTTCATTGCATCCTCTA+CGG - chr5.2:30626533-30626552 None:intergenic 30.0%
!! ATTTTTGGATCAATACGGTT+AGG - chr5.2:30624642-30624661 None:intergenic 30.0%
AACACCAAAAAACTTAGTCC+TGG + chr5.2:30626429-30626448 MS.gene55225:CDS 35.0%
AACAGAAAAATCTGTTACCG+AGG + chr5.2:30625307-30625326 MS.gene55225:CDS 35.0%
AGGATGCCATGAAGATTAAA+TGG + chr5.2:30625426-30625445 MS.gene55225:CDS 35.0%
ATGAAAAAATCAGCTGGAAC+TGG + chr5.2:30625218-30625237 MS.gene55225:CDS 35.0%
CATGTTTACTACTTCCCATT+AGG + chr5.2:30624711-30624730 MS.gene55225:CDS 35.0%
GCCTTACACAATGATATAGA+GGG + chr5.2:30626264-30626283 MS.gene55225:CDS 35.0%
TCCCTCTATATCATTGTGTA+AGG - chr5.2:30626268-30626287 None:intergenic 35.0%
TCGAACAAAAAAGCTTAGGA+TGG + chr5.2:30626230-30626249 MS.gene55225:CDS 35.0%
TGCCTTACACAATGATATAG+AGG + chr5.2:30626263-30626282 MS.gene55225:CDS 35.0%
TGTAGTTAATGCTAAGGTTG+TGG + chr5.2:30625403-30625422 MS.gene55225:CDS 35.0%
TTACACGATTCAGGTATCAA+GGG + chr5.2:30626498-30626517 MS.gene55225:CDS 35.0%
TTTACACGATTCAGGTATCA+AGG + chr5.2:30626497-30626516 MS.gene55225:CDS 35.0%
TTTACTGAGAGTTCTTGAGA+CGG - chr5.2:30625068-30625087 None:intergenic 35.0%
! ACCGTATTGATCCAAAAATC+TGG + chr5.2:30624643-30624662 MS.gene55225:CDS 35.0%
! ATGAAACTCTATCTTTGGGA+TGG - chr5.2:30626298-30626317 None:intergenic 35.0%
! ATTAATTTTGCGCATTCGCT+AGG - chr5.2:30625014-30625033 None:intergenic 35.0%
!! GCCAGATTTTTGGATCAATA+CGG - chr5.2:30624647-30624666 None:intergenic 35.0%
!!! AAGTTTTTTGGTGTTGCAGT+TGG - chr5.2:30626424-30626443 None:intergenic 35.0%
!!! ATCCTAAGCTTTTTTGTTCG+AGG - chr5.2:30626231-30626250 None:intergenic 35.0%
!!! TCCTAAGCTTTTTTGTTCGA+GGG - chr5.2:30626230-30626249 None:intergenic 35.0%
!!! TGAACCAGGACTAAGTTTTT+TGG - chr5.2:30626436-30626455 None:intergenic 35.0%
AACAAACTCACTCCAGTTAG+TGG - chr5.2:30625159-30625178 None:intergenic 40.0%
ACTCCGTCTGATGCTAATAA+CGG + chr5.2:30624972-30624991 MS.gene55225:CDS 40.0%
AGTTAATGCTAAGGTTGTGG+AGG + chr5.2:30625406-30625425 MS.gene55225:CDS 40.0%
ATTATCCTACTGCTGAAGGT+TGG + chr5.2:30625372-30625391 MS.gene55225:CDS 40.0%
CTCTCAGTAAAGGACATTCA+CGG + chr5.2:30625077-30625096 MS.gene55225:CDS 40.0%
GGAAGTGATTTACACGATTC+AGG + chr5.2:30626489-30626508 MS.gene55225:CDS 40.0%
GTCTCAAGAACTCTCAGTAA+AGG + chr5.2:30625067-30625086 MS.gene55225:CDS 40.0%
GTGTATTATCCTACTGCTGA+AGG + chr5.2:30625368-30625387 MS.gene55225:CDS 40.0%
GTTGACGAGAAGAAACTGAT+TGG + chr5.2:30625176-30625195 MS.gene55225:CDS 40.0%
TACACGATTCAGGTATCAAG+GGG + chr5.2:30626499-30626518 MS.gene55225:CDS 40.0%
TATCTGTTCCTAATCGTCCA+TGG + chr5.2:30625534-30625553 MS.gene55225:CDS 40.0%
TCCCTCGAACAAAAAAGCTT+AGG + chr5.2:30626226-30626245 MS.gene55225:CDS 40.0%
TCGTGTAAATCACTTCCAAC+AGG - chr5.2:30626486-30626505 None:intergenic 40.0%
TGGATGTAGTGCATGTATGT+TGG - chr5.2:30626202-30626221 None:intergenic 40.0%
TTCGGAAAAAAGATTGTGCC+TGG - chr5.2:30626391-30626410 None:intergenic 40.0%
! ATGTGTTCTAAGTGCCCTAA+TGG - chr5.2:30624729-30624748 None:intergenic 40.0%
! GCAGTTGGAAGAAGTTGATT+CGG - chr5.2:30626409-30626428 None:intergenic 40.0%
! TGTGTTCTAAGTGCCCTAAT+GGG - chr5.2:30624728-30624747 None:intergenic 40.0%
! TTTTCAACTTGGAGGTCAAG+TGG - chr5.2:30625044-30625063 None:intergenic 40.0%
!! AAACTCTATCTTTGGGATGG+AGG - chr5.2:30626295-30626314 None:intergenic 40.0%
!! TGGAATTTTAAAGGCAGACC+CGG - chr5.2:30624679-30624698 None:intergenic 40.0%
!!! TTTTTTGTTCGAGGGAACTG+TGG - chr5.2:30626222-30626241 None:intergenic 40.0%
AAAATCTGTTACCGAGGCTG+TGG + chr5.2:30625313-30625332 MS.gene55225:CDS 45.0%
ACACGATTCAGGTATCAAGG+GGG + chr5.2:30626500-30626519 MS.gene55225:CDS 45.0%
AGGACATTCACGGCAAAGAA+TGG + chr5.2:30625087-30625106 MS.gene55225:CDS 45.0%
CCACCGTTATTAGCATCAGA+CGG - chr5.2:30624978-30624997 None:intergenic 45.0%
GAAATAAGCTCAACCTCCCA+AGG - chr5.2:30626180-30626199 None:intergenic 45.0%
GAATGAATGGACGAGAACCA+TGG - chr5.2:30624788-30624807 None:intergenic 45.0%
GACGAGAAGAAACTGATTGG+TGG + chr5.2:30625179-30625198 MS.gene55225:CDS 45.0%
GATTCCAACGGAGATCGTTA+CGG - chr5.2:30625255-30625274 None:intergenic 45.0%
GGAATTGGCGGAGAAGAATA+TGG + chr5.2:30625334-30625353 MS.gene55225:CDS 45.0%
GTTCGTGAAGACGAAGATGA+CGG + chr5.2:30624918-30624937 MS.gene55225:CDS 45.0%
TGAACCTAAAGTTTCGCAGC+TGG + chr5.2:30626137-30626156 MS.gene55225:CDS 45.0%
TTCGTGAAGACGAAGATGAC+GGG + chr5.2:30624919-30624938 MS.gene55225:CDS 45.0%
! CTTGAGACGGCTTTTCAACT+TGG - chr5.2:30625055-30625074 None:intergenic 45.0%
! TCAATACGGTTAGGTTGCTG+AGG - chr5.2:30624633-30624652 None:intergenic 45.0%
! TTTTGCGCATTCGCTAGGTA+CGG - chr5.2:30625009-30625028 None:intergenic 45.0%
ATGGACGAGAACCATGGAGA+AGG - chr5.2:30624782-30624801 None:intergenic 50.0%
CACTCCAGTTAGTGGTGATC+AGG - chr5.2:30625151-30625170 None:intergenic 50.0%
CCGTCTGATGCTAATAACGG+TGG + chr5.2:30624975-30624994 MS.gene55225:CDS 50.0%
GACACCTGATCACCACTAAC+TGG + chr5.2:30625144-30625163 MS.gene55225:CDS 50.0%
GAGATGGTGCAGGGAATGAA+TGG - chr5.2:30624801-30624820 None:intergenic 50.0%
GCTTTGCTCGATGACATGCA+GGG + chr5.2:30626363-30626382 MS.gene55225:CDS 50.0%
GGAACCAGCTGCGAAACTTT+AGG - chr5.2:30626144-30626163 None:intergenic 50.0%
GGTCAGATCATACGTCCTGT+TGG + chr5.2:30626468-30626487 MS.gene55225:CDS 50.0%
TATACCTCAAGCATGCGCCA+TGG - chr5.2:30625554-30625573 None:intergenic 50.0%
TCAACTTGGAGGTCAAGTGG+CGG - chr5.2:30625041-30625060 None:intergenic 50.0%
TCACACCAACCTTCAGCAGT+AGG - chr5.2:30625380-30625399 None:intergenic 50.0%
TCGTGAAGACGAAGATGACG+GGG + chr5.2:30624920-30624939 MS.gene55225:CDS 50.0%
TCTTCGTCTTCACGAACCTC+AGG - chr5.2:30624915-30624934 None:intergenic 50.0%
TGCTTTGCTCGATGACATGC+AGG + chr5.2:30626362-30626381 MS.gene55225:CDS 50.0%
TGGACGAGAACCATGGAGAA+GGG - chr5.2:30624781-30624800 None:intergenic 50.0%
TGTTACCGAGGCTGTGGAAT+TGG + chr5.2:30625319-30625338 MS.gene55225:CDS 50.0%
! CGGAGTTAGAGTTTTGCCAG+AGG - chr5.2:30624958-30624977 None:intergenic 50.0%
! GAGACGGCTTTTCAACTTGG+AGG - chr5.2:30625052-30625071 None:intergenic 50.0%
AAAATCTGGCAGTGCTGCGC+CGG + chr5.2:30624657-30624676 MS.gene55225:CDS 55.0%
ACTGCCGTAACGATCTCCGT+TGG + chr5.2:30625248-30625267 MS.gene55225:CDS 55.0%
AGCACACTCTCCCTTCTCCA+TGG + chr5.2:30624768-30624787 MS.gene55225:CDS 55.0%
AGCATGCGCCATGGACGATT+AGG - chr5.2:30625545-30625564 None:intergenic 55.0%
AGTGTGCTAGGGTTTGGGGA+TGG - chr5.2:30624756-30624775 None:intergenic 55.0%
CAACTTGGAGGTCAAGTGGC+GGG - chr5.2:30625040-30625059 None:intergenic 55.0%
CGATTCAGGTATCAAGGGGG+AGG + chr5.2:30626503-30626522 MS.gene55225:CDS 55.0%
GAGTAGATCAACCGCGGAGA+TGG - chr5.2:30624817-30624836 None:intergenic 55.0%
GTCAGCGAGTAGATCAACCG+CGG - chr5.2:30624823-30624842 None:intergenic 55.0%
GTGGACACGGTCGTTAGTGA+CGG - chr5.2:30624886-30624905 None:intergenic 55.0%
TACCGAGGCTGTGGAATTGG+CGG + chr5.2:30625322-30625341 MS.gene55225:CDS 55.0%
TCAGGTATCAAGGGGGAGGA+TGG + chr5.2:30626507-30626526 MS.gene55225:CDS 55.0%
TCCAGAGCGAGTCAGTCCTT+GGG + chr5.2:30626161-30626180 MS.gene55225:CDS 55.0%
TCGTCTTCACGAACCTCAGG+AGG - chr5.2:30624912-30624931 None:intergenic 55.0%
TGGACACGGTCGTTAGTGAC+GGG - chr5.2:30624885-30624904 None:intergenic 55.0%
TGGCAGCACCAAACTTCTGC+CGG - chr5.2:30625280-30625299 None:intergenic 55.0%
TTCCAGAGCGAGTCAGTCCT+TGG + chr5.2:30626160-30626179 MS.gene55225:CDS 55.0%
! AGGGAGAGTGTGCTAGGGTT+TGG - chr5.2:30624762-30624781 None:intergenic 55.0%
! AGTGGTGATCAGGTGTCGCA+AGG - chr5.2:30625141-30625160 None:intergenic 55.0%
! GCGCAGCACTGCCAGATTTT+TGG - chr5.2:30624657-30624676 None:intergenic 55.0%
! GGAGAAGGGAGAGTGTGCTA+GGG - chr5.2:30624767-30624786 None:intergenic 55.0%
! GGAGAGTGTGCTAGGGTTTG+GGG - chr5.2:30624760-30624779 None:intergenic 55.0%
! GGGAGAGTGTGCTAGGGTTT+GGG - chr5.2:30624761-30624780 None:intergenic 55.0%
! GGGGTTGAGGTTGTTTCCTC+TGG + chr5.2:30624939-30624958 MS.gene55225:CDS 55.0%
! GGTCGTTAGTGACGGGAGTT+AGG - chr5.2:30624878-30624897 None:intergenic 55.0%
! GTGGTGATCAGGTGTCGCAA+GGG - chr5.2:30625140-30625159 None:intergenic 55.0%
! TGGAGAAGGGAGAGTGTGCT+AGG - chr5.2:30624768-30624787 None:intergenic 55.0%
!! AGACGAAGATGACGGGGTTG+AGG + chr5.2:30624926-30624945 MS.gene55225:CDS 55.0%
AAATCTGGCAGTGCTGCGCC+GGG + chr5.2:30624658-30624677 MS.gene55225:CDS 60.0%
AAGCTTCGCCACGTCTACCG+TGG + chr5.2:30625110-30625129 MS.gene55225:CDS 60.0%
AGAGCGAGTCAGTCCTTGGG+AGG + chr5.2:30626164-30626183 MS.gene55225:CDS 60.0%
AGCTTCGCCACGTCTACCGT+GGG + chr5.2:30625111-30625130 MS.gene55225:CDS 60.0%
ATCAACCGCGGAGATGGTGC+AGG - chr5.2:30624811-30624830 None:intergenic 60.0%
CACGAACCTCAGGAGGTTCG+TGG - chr5.2:30624905-30624924 None:intergenic 60.0%
CAGCACCAAACTTCTGCCGG+CGG - chr5.2:30625277-30625296 None:intergenic 60.0%
CATTCCCTGCACCATCTCCG+CGG + chr5.2:30624803-30624822 MS.gene55225:CDS 60.0%
CCTCAGGAGGTTCGTGGACA+CGG - chr5.2:30624899-30624918 None:intergenic 60.0%
CGATCTCCGTTGGAATCCGC+CGG + chr5.2:30625258-30625277 MS.gene55225:CDS 60.0%
CTCCGCCAATTCCACAGCCT+CGG - chr5.2:30625327-30625346 None:intergenic 60.0%
GGAATCCGCCGGCAGAAGTT+TGG + chr5.2:30625269-30625288 MS.gene55225:CDS 60.0%
TCAACCGCGGAGATGGTGCA+GGG - chr5.2:30624810-30624829 None:intergenic 60.0%
TCGATGACATGCAGGGAGCC+AGG + chr5.2:30626370-30626389 MS.gene55225:CDS 60.0%
TCGTCCATGGCGCATGCTTG+AGG + chr5.2:30625547-30625566 MS.gene55225:CDS 60.0%
! CTTCTGCCGGCGGATTCCAA+CGG - chr5.2:30625267-30625286 None:intergenic 60.0%
!! TCCCAAGGACTGACTCGCTC+TGG - chr5.2:30626165-30626184 None:intergenic 60.0%
AAGGGGGAGGATGGCCGTAG+AGG + chr5.2:30626516-30626535 MS.gene55225:CDS 65.0%
AGGGAGTCCCACGGTAGACG+TGG - chr5.2:30625121-30625140 None:intergenic 65.0%
CCGTGTCCACGAACCTCCTG+AGG + chr5.2:30624896-30624915 MS.gene55225:CDS 65.0%
! GGTGTCGCAAGGGAGTCCCA+CGG - chr5.2:30625130-30625149 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 30624624 30626563 30624624 ID=MS.gene55225
chr5.2 mRNA 30624624 30626563 30624624 ID=MS.gene55225.t1;Parent=MS.gene55225
chr5.2 exon 30624624 30625568 30624624 ID=MS.gene55225.t1.exon1;Parent=MS.gene55225.t1
chr5.2 CDS 30624624 30625568 30624624 ID=cds.MS.gene55225.t1;Parent=MS.gene55225.t1
chr5.2 exon 30626126 30626563 30626126 ID=MS.gene55225.t1.exon2;Parent=MS.gene55225.t1
chr5.2 CDS 30626126 30626563 30626126 ID=cds.MS.gene55225.t1;Parent=MS.gene55225.t1
Gene Sequence

>MS.gene55225

ATGTCTCCTCAGCAACCTAACCGTATTGATCCAAAAATCTGGCAGTGCTGCGCCGGGTCTGCCTTTAAAATTCCAAAACTACATTCTCATGTTTACTACTTCCCATTAGGGCACTTAGAACACATATGTCCATCCCCAAACCCTAGCACACTCTCCCTTCTCCATGGTTCTCGTCCATTCATTCCCTGCACCATCTCCGCGGTTGATCTACTCGCTGACTCTCGTACTGACGAAGTCTTCGTCAAACTACTCCTAACTCCCGTCACTAACGACCGTGTCCACGAACCTCCTGAGGTTCGTGAAGACGAAGATGACGGGGTTGAGGTTGTTTCCTCTGGCAAAACTCTAACTCCGTCTGATGCTAATAACGGTGGTGCATTCTCCGTACCTAGCGAATGCGCAAAATTAATCTTCCCGCCACTTGACCTCCAAGTTGAAAAGCCGTCTCAAGAACTCTCAGTAAAGGACATTCACGGCAAAGAATGGAAGCTTCGCCACGTCTACCGTGGGACTCCCTTGCGACACCTGATCACCACTAACTGGAGTGAGTTTGTTGACGAGAAGAAACTGATTGGTGGTGATTCCTTAGTTTTCATGAAAAAATCAGCTGGAACTGGAACTGAAACTGCCGTAACGATCTCCGTTGGAATCCGCCGGCAGAAGTTTGGTGCTGCCACTAAGATAACAGAAAAATCTGTTACCGAGGCTGTGGAATTGGCGGAGAAGAATATGGCTTTCGAAGTTGTGTATTATCCTACTGCTGAAGGTTGGTGTGATTTTGTAGTTAATGCTAAGGTTGTGGAGGATGCCATGAAGATTAAATGGAATTCTGGATTGAGAATTAAACTTTCTTTGAAGAAAGACAATTCTTCAAAGAGATGCTCAAATTTTGAGGGAACAATATCTGCTCTATCTGTTCCTAATCGTCCATGGCGCATGCTTGAGGTATATATTTATATATGCTCTCTTCTTTTAGTCTTGTTATATTTCAACTGCACAACGTTATATGTAGTATTTTTGTTTAATAAATGTTATCGGTCAAATTTATGGTTCATGTTAGGAACTATATTTTTTTGATCAGTTCATGTTAGGAACTATATGTTCCAATGTGAAAAATATAGGATATGTTCCAATGTGTTAATCTTAAACATTAATGTTGTGCAATTAAATTTAAGTGGCTTAATTCCTTTTTTATGAGTCATATCGTACCACATCATGAATTAACTACAATGACTTTCTTCCTCAAAAAAAACTACAATGACTTTAGAAACTTTATACTTGAAATAGGTCGACTAAGATTTTTTCTTTTTTTGAGCAAATGGTCGAATAAGTTGTCTCACTTGTGTGTTTACATATGTATTTGTACAATATACTTTATTGGATTAAATAAGTTTTTCGTCCCTATAAATATAGCGAATTTTGATTTTAATCCTTAAAAAATAAAATGAGATGTTTTAAATGATTGATACGGATACTTATCATTTTTCATGTCAATATCATAGGTTAATTGGGATGAACCTAAAGTTTCGCAGCTGGTTCCAGAGCGAGTCAGTCCTTGGGAGGTTGAGCTTATTTCCAACATACATGCACTACATCCACAGTTCCCTCGAACAAAAAAGCTTAGGATGGCTAAAGATTCTGCCTTACACAATGATATAGAGGGAGACTCCTCCATCCCAAAGATAGAGTTTCATTCTTCAGCGACGAGTTCCTTCAATGAAACATCGTTGAATAGCAATGCTTTGCTCGATGACATGCAGGGAGCCAGGCACAATCTTTTTTCCGAATCAACTTCTTCCAACTGCAACACCAAAAAACTTAGTCCTGGTTCAATTATGTTATTTGGTCAGATCATACGTCCTGTTGGAAGTGATTTACACGATTCAGGTATCAAGGGGGAGGATGGCCGTAGAGGATGCAATGAAAATAGTCTTGTGTAG
Protein sequence

>MS.gene55225.t1

MSPQQPNRIDPKIWQCCAGSAFKIPKLHSHVYYFPLGHLEHICPSPNPSTLSLLHGSRPFIPCTISAVDLLADSRTDEVFVKLLLTPVTNDRVHEPPEVREDEDDGVEVVSSGKTLTPSDANNGGAFSVPSECAKLIFPPLDLQVEKPSQELSVKDIHGKEWKLRHVYRGTPLRHLITTNWSEFVDEKKLIGGDSLVFMKKSAGTGTETAVTISVGIRRQKFGAATKITEKSVTEAVELAEKNMAFEVVYYPTAEGWCDFVVNAKVVEDAMKIKWNSGLRIKLSLKKDNSSKRCSNFEGTISALSVPNRPWRMLEVNWDEPKVSQLVPERVSPWEVELISNIHALHPQFPRTKKLRMAKDSALHNDIEGDSSIPKIEFHSSATSSFNETSLNSNALLDDMQGARHNLFSESTSSNCNTKKLSPGSIMLFGQIIRPVGSDLHDSGIKGEDGRRGCNENSLV