AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55256

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55256.t1 MTR_5g041530 84.974 386 43 2 1 376 1 381 0.0 620
MS.gene55256.t1 MTR_5g041380 61.461 397 106 10 1 376 1 371 1.42e-150 430
MS.gene55256.t1 MTR_5g041400 60.506 395 105 10 1 376 1 363 2.11e-147 421
MS.gene55256.t1 MTR_5g041420 69.836 305 70 5 1 287 1 301 7.44e-131 378
MS.gene55256.t1 MTR_4g082060 39.876 484 148 16 7 375 9 464 3.12e-85 266
MS.gene55256.t1 MTR_6g012450 35.867 421 143 12 80 374 74 493 7.80e-65 214
MS.gene55256.t1 MTR_7g010950 41.736 242 105 5 2 219 4 233 1.62e-42 155
MS.gene55256.t1 MTR_7g010950 39.306 173 75 5 230 374 314 484 2.19e-24 104
MS.gene55256.t1 MTR_3g435480 48.571 140 65 3 69 205 66 201 3.05e-32 126
MS.gene55256.t1 MTR_3g435480 45.578 147 56 5 242 374 327 463 6.00e-25 105
MS.gene55256.t1 MTR_6g027460 56.566 99 40 1 98 193 51 149 4.04e-32 124
MS.gene55256.t1 MTR_8g044220 46.218 119 58 1 117 229 95 213 8.57e-32 125
MS.gene55256.t1 MTR_8g044220 33.793 145 69 5 248 376 306 439 7.95e-12 66.6
MS.gene55256.t1 MTR_7g086780 41.420 169 71 3 77 217 35 203 8.84e-32 124
MS.gene55256.t1 MTR_6g027450 55.208 96 40 1 101 193 47 142 6.23e-30 117
MS.gene55256.t1 MTR_2g016030 77.966 59 13 0 135 193 42 100 1.74e-29 112
MS.gene55256.t1 MTR_8g015840 60.274 73 25 1 133 201 9 81 3.07e-24 99.8
MS.gene55256.t1 MTR_2g096740 41.538 130 69 2 92 221 31 153 1.02e-23 100
MS.gene55256.t1 MTR_2g096740 50.000 98 44 1 124 221 46 138 1.50e-23 99.4
MS.gene55256.t1 MTR_8g479350 52.083 96 41 2 142 233 70 164 4.97e-23 99.0
MS.gene55256.t1 MTR_4g022370 56.522 69 30 0 125 193 67 135 1.29e-22 98.2
MS.gene55256.t1 MTR_3g091820 58.209 67 28 0 127 193 30 96 3.25e-22 95.9
MS.gene55256.t1 MTR_4g063780 46.789 109 53 3 136 241 71 177 5.17e-22 95.9
MS.gene55256.t1 MTR_8g079060 69.811 53 16 0 140 192 29 81 6.71e-22 93.6
MS.gene55256.t1 MTR_2g059540 70.588 51 15 0 138 188 29 79 8.68e-22 92.4
MS.gene55256.t1 MTR_4g109980 69.231 52 16 0 139 190 39 90 1.12e-21 94.7
MS.gene55256.t1 MTR_8g068210 52.703 74 34 1 122 194 14 87 1.20e-21 95.1
MS.gene55256.t1 MTR_8g027295 62.712 59 22 0 135 193 19 77 1.39e-21 93.6
MS.gene55256.t1 MTR_7g024670 61.538 65 24 1 142 205 79 143 1.45e-21 95.1
MS.gene55256.t1 MTR_2g093220 58.571 70 28 1 125 193 24 93 2.04e-21 93.6
MS.gene55256.t1 MTR_3g090430 57.534 73 29 2 127 197 36 108 2.36e-21 94.0
MS.gene55256.t1 MTR_1g077600 55.072 69 31 0 127 195 30 98 2.59e-21 92.8
MS.gene55256.t1 MTR_1g077600 55.072 69 31 0 127 195 30 98 3.34e-21 92.8
MS.gene55256.t1 MTR_4g088580 71.154 52 15 0 142 193 57 108 1.70e-20 92.4
MS.gene55256.t1 MTR_1g056810 53.684 95 38 4 131 221 2 94 2.28e-20 90.5
MS.gene55256.t1 MTR_7g059400 60.656 61 23 1 133 193 70 129 4.24e-20 90.9
MS.gene55256.t1 MTR_4g461080 60.000 65 25 1 129 192 17 81 4.64e-20 89.4
MS.gene55256.t1 MTR_5g031440 63.636 55 20 0 136 190 39 93 5.44e-20 90.1
MS.gene55256.t1 MTR_4g089095 62.264 53 20 0 142 194 52 104 1.73e-19 88.2
MS.gene55256.t1 MTR_4g089095 62.264 53 20 0 142 194 18 70 2.13e-19 87.4
MS.gene55256.t1 MTR_2g013370 68.519 54 16 1 140 193 46 98 3.14e-19 87.0
MS.gene55256.t1 MTR_3g077750 64.815 54 19 0 137 190 58 111 5.94e-19 87.4
MS.gene55256.t1 MTR_2g014060 66.000 50 17 0 142 191 27 76 8.80e-19 86.7
MS.gene55256.t1 MTR_2g014170 67.347 49 16 0 140 188 45 93 1.22e-18 85.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55256.t1 AT5G62430 43.403 288 124 11 112 376 27 298 5.16e-60 196
MS.gene55256.t1 AT5G39660 36.829 391 152 12 70 374 74 455 2.07e-56 191
MS.gene55256.t1 AT5G39660 36.829 391 152 12 70 374 74 455 2.07e-56 191
MS.gene55256.t1 AT5G39660 36.829 391 152 12 70 374 74 455 2.07e-56 191
MS.gene55256.t1 AT1G69570 36.458 288 120 13 135 376 127 397 1.24e-40 148
MS.gene55256.t1 AT1G26790 34.650 329 146 16 96 376 65 372 2.08e-35 133
MS.gene55256.t1 AT1G29160 73.913 69 15 1 125 193 50 115 1.36e-32 120
MS.gene55256.t1 AT2G34140 58.242 91 26 2 125 206 46 133 1.46e-31 117
MS.gene55256.t1 AT3G47500 43.713 167 54 6 89 228 72 225 9.28e-31 122
MS.gene55256.t1 AT3G47500 42.945 163 70 7 230 376 293 448 4.10e-21 94.7
MS.gene55256.t1 AT5G60200 59.459 74 27 1 133 203 48 121 5.25e-24 100
MS.gene55256.t1 AT2G28810 39.098 133 78 2 108 240 48 177 5.57e-24 101
MS.gene55256.t1 AT2G28810 39.098 133 78 2 108 240 65 194 9.55e-24 100
MS.gene55256.t1 AT1G51700 73.077 52 14 0 140 191 33 84 5.09e-23 95.5
MS.gene55256.t1 AT1G07640 54.545 77 29 1 123 199 10 80 6.02e-23 97.4
MS.gene55256.t1 AT1G07640 54.545 77 29 1 123 199 66 136 9.13e-23 98.2
MS.gene55256.t1 AT1G07640 54.545 77 29 1 123 199 74 144 1.05e-22 98.2
MS.gene55256.t1 AT5G60850 70.370 54 16 0 140 193 51 104 2.65e-22 96.3
MS.gene55256.t1 AT2G37590 54.412 68 31 0 126 193 75 142 2.74e-22 96.7
MS.gene55256.t1 AT3G21270 74.510 51 13 0 142 192 31 81 4.29e-22 93.6
MS.gene55256.t1 AT4G24060 60.000 65 26 0 129 193 42 106 1.56e-21 94.7
MS.gene55256.t1 AT3G52440 47.312 93 48 1 142 233 27 119 1.57e-21 92.8
MS.gene55256.t1 AT3G55370 60.317 63 24 1 131 193 68 129 1.63e-21 94.4
MS.gene55256.t1 AT3G55370 60.317 63 24 1 131 193 68 129 1.99e-21 94.7
MS.gene55256.t1 AT3G52440 47.312 93 48 1 142 233 46 138 2.16e-21 92.8
MS.gene55256.t1 AT3G55370 60.317 63 24 1 131 193 113 174 2.40e-21 94.7
MS.gene55256.t1 AT3G50410 69.231 52 16 0 140 191 30 81 3.19e-21 92.0
MS.gene55256.t1 AT3G61850 57.576 66 28 0 128 193 50 115 4.87e-21 92.4
MS.gene55256.t1 AT3G61850 57.576 66 28 0 128 193 50 115 4.87e-21 92.4
MS.gene55256.t1 AT4G38000 53.333 75 34 1 139 212 40 114 5.56e-21 91.3
MS.gene55256.t1 AT3G61850 57.576 66 28 0 128 193 62 127 5.72e-21 92.4
MS.gene55256.t1 AT3G45610 53.425 73 32 1 133 203 33 105 6.33e-21 91.3
MS.gene55256.t1 AT3G61850 57.576 66 28 0 128 193 50 115 6.83e-21 92.4
MS.gene55256.t1 AT3G61850 57.576 66 28 0 128 193 62 127 7.75e-21 92.4
MS.gene55256.t1 AT5G65590 66.667 54 18 0 140 193 41 94 1.50e-20 91.7
MS.gene55256.t1 AT5G02460 62.069 58 22 0 136 193 91 148 3.52e-20 91.7
MS.gene55256.t1 AT1G28310 66.667 54 18 0 140 193 27 80 4.52e-20 90.1
MS.gene55256.t1 AT2G28510 67.308 52 17 0 142 193 50 101 5.28e-20 89.4
MS.gene55256.t1 AT1G28310 66.667 54 18 0 140 193 51 104 6.20e-20 90.1
MS.gene55256.t1 AT5G66940 66.667 51 17 0 142 192 34 84 9.16e-20 87.4
MS.gene55256.t1 AT1G21340 61.404 57 22 0 142 198 40 96 1.62e-19 87.4
MS.gene55256.t1 AT4G00940 55.556 72 27 1 127 193 50 121 1.64e-19 88.2
MS.gene55256.t1 AT4G00940 55.556 72 27 1 127 193 50 121 1.64e-19 88.2
MS.gene55256.t1 AT4G00940 55.556 72 27 1 127 193 50 121 1.64e-19 88.2
MS.gene55256.t1 AT1G64620 56.667 60 26 0 129 188 38 97 1.84e-19 89.0
MS.gene55256.t1 AT1G47655 65.306 49 17 0 140 188 29 77 4.83e-19 85.1
MS.gene55256.t1 AT4G21050 67.308 52 17 0 142 193 26 77 8.34e-19 84.3
MS.gene55256.t1 AT2G46590 60.377 53 21 0 135 187 63 115 5.08e-18 84.7
MS.gene55256.t1 AT2G46590 60.377 53 21 0 135 187 75 127 5.28e-18 85.1
MS.gene55256.t1 AT5G62940 67.347 49 16 0 142 190 75 123 6.23e-18 84.7
MS.gene55256.t1 AT4G21040 61.538 52 20 0 142 193 27 78 6.81e-17 79.7
MS.gene55256.t1 AT4G21080 47.436 78 38 2 142 216 27 104 2.19e-16 78.6

Find 73 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACTATTATTATTTCTTTC+TGG 0.141171 5.2:-31086946 None:intergenic
TCATCAATTCTCAGTGTCTT+TGG 0.267736 5.2:-31086976 None:intergenic
ACCACCATAGTATGCTATAT+TGG 0.313954 5.2:-31086791 None:intergenic
CATCAATTCTCAGTGTCTTT+GGG 0.324243 5.2:-31086975 None:intergenic
ACAATTAGGTCTGGTTTCAT+TGG 0.366902 5.2:-31086445 None:intergenic
TGCCAGAGGTATTGGACTTC+CGG 0.368367 5.2:+31086556 MS.gene55256:CDS
TCTTCCTTAGAGTTAGAGTT+TGG 0.372291 5.2:+31085287 MS.gene55256:CDS
AGCATACTATGGTGGTTGTT+TGG 0.375936 5.2:+31086798 MS.gene55256:CDS
CAGTTCAGAAGTTTCTGTTT+CGG 0.385660 5.2:-31086374 None:intergenic
TCGGTTGGAGAATTCACTTC+AGG 0.387221 5.2:-31085371 None:intergenic
CCACCATAGTATGCTATATT+GGG 0.409801 5.2:-31086790 None:intergenic
ATGTTCCTCCACAGTTTCCT+TGG 0.411126 5.2:+31086734 MS.gene55256:CDS
TATCCCAATATAGCATACTA+TGG 0.411753 5.2:+31086787 MS.gene55256:CDS
GGGGAACATGAAGATGAATA+AGG 0.417962 5.2:-31085347 None:intergenic
AGTGAAGTTGAAGACGTAAT+AGG 0.418827 5.2:-31086685 None:intergenic
AGTACTACTATCTTGACCTT+TGG 0.421634 5.2:+31086652 MS.gene55256:CDS
AGAACCAAACTCTAACTCTA+AGG 0.422367 5.2:-31085291 None:intergenic
AAATGATGGAAAGAGATATA+AGG 0.429316 5.2:+31085394 MS.gene55256:CDS
TAACTCTAAGGAAGAATCAT+TGG 0.432927 5.2:-31085279 None:intergenic
TATTATTTCTTTCTGGTCCA+AGG 0.433279 5.2:-31086939 None:intergenic
GACACAGGATAACACATTGC+AGG 0.442835 5.2:-31086760 None:intergenic
CATATTTCTCGTGGCTCCTC+CGG 0.451801 5.2:-31086575 None:intergenic
GGACAAGGAACAATTAGGTC+TGG 0.461533 5.2:-31086454 None:intergenic
TTCATGGGATTTGCATCCAT+AGG 0.466445 5.2:+31087063 MS.gene55256:CDS
TCATGGGATTTGCATCCATA+GGG 0.470010 5.2:+31087064 MS.gene55256:CDS
CTCCGGAAGTCCAATACCTC+TGG 0.499281 5.2:-31086558 None:intergenic
GGTCAAAATTCAACTACAGT+AGG 0.501685 5.2:+31086862 MS.gene55256:CDS
AGAAGTTTCTGTTTCGGACG+AGG 0.503415 5.2:-31086368 None:intergenic
CTTGACAAGAAAGTGAATGT+TGG 0.504154 5.2:+31086706 MS.gene55256:CDS
TTCCCAATCAAATCCTCTCA+TGG 0.505431 5.2:-31087164 None:intergenic
ATCTTGGACAAGGAACAATT+AGG 0.509108 5.2:-31086459 None:intergenic
AATTCTCCAACCGAAAATGA+TGG 0.512006 5.2:+31085380 MS.gene55256:CDS
AAATTGCGAAGAGTTCTATA+TGG 0.520045 5.2:+31087007 MS.gene55256:CDS
CTAATCCTGTAGCTTTATCA+AGG 0.525653 5.2:+31087130 MS.gene55256:CDS
AGAAATATGATTGTAGGTGC+AGG 0.532367 5.2:+31086589 MS.gene55256:CDS
GAAGTTTCTGTTTCGGACGA+GGG 0.536733 5.2:-31086367 None:intergenic
GCCACGAGAAATATGATTGT+AGG 0.539882 5.2:+31086583 MS.gene55256:CDS
AGAGACCTTGATAAAGCTAC+AGG 0.544478 5.2:-31087135 None:intergenic
AACAAACTCAATGCAGCCAA+TGG 0.545923 5.2:+31086622 MS.gene55256:CDS
GTCAAAATTCAACTACAGTA+GGG 0.548037 5.2:+31086863 MS.gene55256:CDS
GTAGGGAAACATTCAAGAGA+TGG 0.550990 5.2:+31086880 MS.gene55256:CDS
GGTCTCTTGTGTTCCATGAG+AGG 0.551572 5.2:+31087151 MS.gene55256:CDS
ACCAATTACAGGAAACATCA+AGG 0.556284 5.2:+31086245 MS.gene55256:intron
GTGGTTGTTTGGCACCATCA+TGG 0.563906 5.2:+31086809 MS.gene55256:CDS
AGTAGTACTACGCAATCCAT+TGG 0.564593 5.2:-31086638 None:intergenic
TAACACATTGCAGGGATCCA+AGG 0.564846 5.2:-31086751 None:intergenic
GTTGGAGAATTCACTTCAGG+TGG 0.567909 5.2:-31085368 None:intergenic
TAGGGAAACATTCAAGAGAT+GGG 0.573118 5.2:+31086881 MS.gene55256:CDS
ATCCTCCGTAGTTTGGAATG+AGG 0.574482 5.2:-31086299 None:intergenic
TGAATAAGGACTATGATCAA+AGG 0.577518 5.2:-31085333 None:intergenic
CACATCGATTCAAGAGGATG+AGG 0.588520 5.2:+31086276 MS.gene55256:CDS
TGAGGCCTCATTCCAAACTA+CGG 0.592028 5.2:+31086294 MS.gene55256:CDS
ATGGGATTTGCATCCATAGG+GGG 0.596431 5.2:+31087066 MS.gene55256:CDS
TTGGAGAATTCACTTCAGGT+GGG 0.604331 5.2:-31085367 None:intergenic
AACTCAGAGACACAAAACCT+TGG 0.606456 5.2:+31086922 MS.gene55256:CDS
TTGTCCAAGATGCAAAAGCA+TGG 0.611424 5.2:+31086471 MS.gene55256:CDS
AATAGGAGAATCTGATCCAA+AGG 0.611763 5.2:-31086668 None:intergenic
ACACAGGATAACACATTGCA+GGG 0.613977 5.2:-31086759 None:intergenic
ACCTACAATCATATTTCTCG+TGG 0.624478 5.2:-31086584 None:intergenic
AGTTCTATATGGTCAACACT+AGG 0.631297 5.2:+31087018 MS.gene55256:CDS
AGAACTCACATCGATTCAAG+AGG 0.638205 5.2:+31086270 MS.gene55256:CDS
CCCAATATAGCATACTATGG+TGG 0.644675 5.2:+31086790 MS.gene55256:CDS
TGGAGAATTCACTTCAGGTG+GGG 0.648645 5.2:-31085366 None:intergenic
TCTATCTCAACACAATCTTG+TGG 0.657997 5.2:+31086841 MS.gene55256:CDS
ATATTGGGATAGAATGACAC+AGG 0.661691 5.2:-31086775 None:intergenic
CATGGGATTTGCATCCATAG+GGG 0.662122 5.2:+31087065 MS.gene55256:CDS
GGGATCCAAGGAAACTGTGG+AGG 0.662521 5.2:-31086739 None:intergenic
ATAGATTGTACATTCCATGA+TGG 0.678301 5.2:-31086823 None:intergenic
GCAGGGATCCAAGGAAACTG+TGG 0.679101 5.2:-31086742 None:intergenic
CAGAGGTATTGGACTTCCGG+AGG 0.686773 5.2:+31086559 MS.gene55256:CDS
GGCCTCATTCCAAACTACGG+AGG 0.721138 5.2:+31086297 MS.gene55256:CDS
TCAACACTAGGAATCAAGAA+CGG 0.728427 5.2:+31087030 MS.gene55256:CDS
ACACTAGGAATCAAGAACGG+AGG 0.738940 5.2:+31087033 MS.gene55256:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATTAAAATATCAAGA+AGG + chr5.2:31085938-31085957 MS.gene55256:intron 10.0%
!! AGAAATTAGAAATTGTTAAT+GGG + chr5.2:31085527-31085546 MS.gene55256:intron 15.0%
!! ATATACAACAGAATATTATA+TGG - chr5.2:31085965-31085984 None:intergenic 15.0%
!! CAGAAATTAGAAATTGTTAA+TGG + chr5.2:31085526-31085545 MS.gene55256:intron 20.0%
!! TTATTGTTAATGATCTTACA+TGG + chr5.2:31085818-31085837 MS.gene55256:intron 20.0%
!!! ACACATTTAATCTAAAGTAT+TGG - chr5.2:31086033-31086052 None:intergenic 20.0%
!!! ACACTATTATTATTTCTTTC+TGG - chr5.2:31086949-31086968 None:intergenic 20.0%
!!! ACTTTGTTCATCAATTTTAT+GGG - chr5.2:31086404-31086423 None:intergenic 20.0%
! GTTATTCATTGTGATTAAAC+CGG - chr5.2:31085773-31085792 None:intergenic 25.0%
! TCATGATTTATTGACGATTT+CGG - chr5.2:31085726-31085745 None:intergenic 25.0%
!! AAATGATGGAAAGAGATATA+AGG + chr5.2:31085394-31085413 MS.gene55256:CDS 25.0%
!! AATTCAACCATAAAGCTTTT+TGG + chr5.2:31085218-31085237 MS.gene55256:CDS 25.0%
!! ATATCTCTTTCCATCATTTT+CGG - chr5.2:31085393-31085412 None:intergenic 25.0%
!! ATTCAACCATAAAGCTTTTT+GGG + chr5.2:31085219-31085238 MS.gene55256:CDS 25.0%
!! CACTTTGTTCATCAATTTTA+TGG - chr5.2:31086405-31086424 None:intergenic 25.0%
!!! TTAGTTTTCATGATGATAGA+TGG + chr5.2:31085658-31085677 MS.gene55256:intron 25.0%
AAATTGCGAAGAGTTCTATA+TGG + chr5.2:31087007-31087026 MS.gene55256:CDS 30.0%
ACAAACTTCATCTTAATCAG+TGG + chr5.2:31085847-31085866 MS.gene55256:intron 30.0%
AGTTCTCAAATTAATATCGG+TGG + chr5.2:31086106-31086125 MS.gene55256:intron 30.0%
ATAGATTGTACATTCCATGA+TGG - chr5.2:31086826-31086845 None:intergenic 30.0%
CATATGTATATCCGTAAAAC+CGG + chr5.2:31085751-31085770 MS.gene55256:intron 30.0%
CATGAAAACTAACCAAAAAG+TGG - chr5.2:31085650-31085669 None:intergenic 30.0%
CGCAGTTCTCAAATTAATAT+CGG + chr5.2:31086103-31086122 MS.gene55256:intron 30.0%
GTCAAAATTCAACTACAGTA+GGG + chr5.2:31086863-31086882 MS.gene55256:CDS 30.0%
GTTCTCAAATTAATATCGGT+GGG + chr5.2:31086107-31086126 MS.gene55256:intron 30.0%
TATCCCAATATAGCATACTA+TGG + chr5.2:31086787-31086806 MS.gene55256:CDS 30.0%
TGAATAAGGACTATGATCAA+AGG - chr5.2:31085336-31085355 None:intergenic 30.0%
TTCCTGTAATTGGTATATAG+CGG - chr5.2:31086239-31086258 None:intergenic 30.0%
! AACTCTTCGCAATTTTGTTA+GGG - chr5.2:31087002-31087021 None:intergenic 30.0%
! AAGGAATTTTCATGAGGAAA+AGG - chr5.2:31085317-31085336 None:intergenic 30.0%
! ATTGCTTCTAGTTAAGACTT+AGG + chr5.2:31085576-31085595 MS.gene55256:intron 30.0%
! TAACTCTAAGGAAGAATCAT+TGG - chr5.2:31085282-31085301 None:intergenic 30.0%
! TATTATTTCTTTCTGGTCCA+AGG - chr5.2:31086942-31086961 None:intergenic 30.0%
! TGAATGAACTTTTATCCAGA+AGG - chr5.2:31085881-31085900 None:intergenic 30.0%
! TGATCAAAGGAATTTTCATG+AGG - chr5.2:31085323-31085342 None:intergenic 30.0%
!! ATGAAGTATTGACATAGACA+CGG - chr5.2:31086149-31086168 None:intergenic 30.0%
!! TTGTGATTAAACCGGTTTTA+CGG - chr5.2:31085765-31085784 None:intergenic 30.0%
AACCGCTATATACCAATTAC+AGG + chr5.2:31086234-31086253 MS.gene55256:intron 35.0%
AATAGGAGAATCTGATCCAA+AGG - chr5.2:31086671-31086690 None:intergenic 35.0%
AATTCTCCAACCGAAAATGA+TGG + chr5.2:31085380-31085399 MS.gene55256:CDS 35.0%
ACCAATTACAGGAAACATCA+AGG + chr5.2:31086245-31086264 MS.gene55256:intron 35.0%
ACCACCATAGTATGCTATAT+TGG - chr5.2:31086794-31086813 None:intergenic 35.0%
ACCTACAATCATATTTCTCG+TGG - chr5.2:31086587-31086606 None:intergenic 35.0%
AGAAATATGATTGTAGGTGC+AGG + chr5.2:31086589-31086608 MS.gene55256:CDS 35.0%
AGAACCAAACTCTAACTCTA+AGG - chr5.2:31085294-31085313 None:intergenic 35.0%
AGTACTACTATCTTGACCTT+TGG + chr5.2:31086652-31086671 MS.gene55256:CDS 35.0%
AGTGAAGTTGAAGACGTAAT+AGG - chr5.2:31086688-31086707 None:intergenic 35.0%
AGTTCTATATGGTCAACACT+AGG + chr5.2:31087018-31087037 MS.gene55256:CDS 35.0%
ATATTGGGATAGAATGACAC+AGG - chr5.2:31086778-31086797 None:intergenic 35.0%
ATCTTGGACAAGGAACAATT+AGG - chr5.2:31086462-31086481 None:intergenic 35.0%
CAGTTCAGAAGTTTCTGTTT+CGG - chr5.2:31086377-31086396 None:intergenic 35.0%
CATCAATTCTCAGTGTCTTT+GGG - chr5.2:31086978-31086997 None:intergenic 35.0%
CCACCATAGTATGCTATATT+GGG - chr5.2:31086793-31086812 None:intergenic 35.0%
CTTGACAAGAAAGTGAATGT+TGG + chr5.2:31086706-31086725 MS.gene55256:CDS 35.0%
GGTCAAAATTCAACTACAGT+AGG + chr5.2:31086862-31086881 MS.gene55256:CDS 35.0%
TACATGTATTAGTGTCGTGT+CGG - chr5.2:31086065-31086084 None:intergenic 35.0%
TAGGGAAACATTCAAGAGAT+GGG + chr5.2:31086881-31086900 MS.gene55256:CDS 35.0%
TCAACACTAGGAATCAAGAA+CGG + chr5.2:31087030-31087049 MS.gene55256:CDS 35.0%
TCATCAATTCTCAGTGTCTT+TGG - chr5.2:31086979-31086998 None:intergenic 35.0%
TCCAATAAATAGAACCACCA+TGG - chr5.2:31085439-31085458 None:intergenic 35.0%
TCCTTGATGTTTCCTGTAAT+TGG - chr5.2:31086249-31086268 None:intergenic 35.0%
TCTATCTCAACACAATCTTG+TGG + chr5.2:31086841-31086860 MS.gene55256:CDS 35.0%
TCTTCCTTAGAGTTAGAGTT+TGG + chr5.2:31085287-31085306 MS.gene55256:CDS 35.0%
TGTTCTCCCAAAAAGCTTTA+TGG - chr5.2:31085228-31085247 None:intergenic 35.0%
! ACAATTAGGTCTGGTTTCAT+TGG - chr5.2:31086448-31086467 None:intergenic 35.0%
! ATTTTGAATCCTCCGTAGTT+TGG - chr5.2:31086309-31086328 None:intergenic 35.0%
! GAACTCTTCGCAATTTTGTT+AGG - chr5.2:31087003-31087022 None:intergenic 35.0%
!! CTAATCCTGTAGCTTTATCA+AGG + chr5.2:31087130-31087149 MS.gene55256:CDS 35.0%
!! TGAAGTATTGACATAGACAC+GGG - chr5.2:31086148-31086167 None:intergenic 35.0%
!!! TTGCTGTTTAGACCACTTTT+TGG + chr5.2:31085635-31085654 MS.gene55256:intron 35.0%
AACAAACTCAATGCAGCCAA+TGG + chr5.2:31086622-31086641 MS.gene55256:CDS 40.0%
AACTCAGAGACACAAAACCT+TGG + chr5.2:31086922-31086941 MS.gene55256:CDS 40.0%
ACACAGGATAACACATTGCA+GGG - chr5.2:31086762-31086781 None:intergenic 40.0%
AGAGACCTTGATAAAGCTAC+AGG - chr5.2:31087138-31087157 None:intergenic 40.0%
AGCATACTATGGTGGTTGTT+TGG + chr5.2:31086798-31086817 MS.gene55256:CDS 40.0%
AGTAGTACTACGCAATCCAT+TGG - chr5.2:31086641-31086660 None:intergenic 40.0%
CCCAATATAGCATACTATGG+TGG + chr5.2:31086790-31086809 MS.gene55256:CDS 40.0%
GCCACGAGAAATATGATTGT+AGG + chr5.2:31086583-31086602 MS.gene55256:CDS 40.0%
GGGGAACATGAAGATGAATA+AGG - chr5.2:31085350-31085369 None:intergenic 40.0%
GTAGGGAAACATTCAAGAGA+TGG + chr5.2:31086880-31086899 MS.gene55256:CDS 40.0%
TAAATAGAACCACCATGGCA+TGG - chr5.2:31085434-31085453 None:intergenic 40.0%
TCATGGGATTTGCATCCATA+GGG + chr5.2:31087064-31087083 MS.gene55256:CDS 40.0%
TTCATGGGATTTGCATCCAT+AGG + chr5.2:31087063-31087082 MS.gene55256:CDS 40.0%
TTGGAGAATTCACTTCAGGT+GGG - chr5.2:31085370-31085389 None:intergenic 40.0%
TTGTCCAAGATGCAAAAGCA+TGG + chr5.2:31086471-31086490 MS.gene55256:CDS 40.0%
! ATGCTTTTGCATCTTGGACA+AGG - chr5.2:31086472-31086491 None:intergenic 40.0%
! CTCTTTCCATCATTTTCGGT+TGG - chr5.2:31085389-31085408 None:intergenic 40.0%
! CTTTTATCCAGAAGGACTTC+AGG - chr5.2:31085873-31085892 None:intergenic 40.0%
! GCCATGGTGGTTCTATTTAT+TGG + chr5.2:31085435-31085454 MS.gene55256:intron 40.0%
!! AAGGTTTTGTGTCTCTGAGT+TGG - chr5.2:31086923-31086942 None:intergenic 40.0%
!! AGAACTCACATCGATTCAAG+AGG + chr5.2:31086270-31086289 MS.gene55256:CDS 40.0%
!!! GAGTTTTCAGGTTCTCAACA+AGG - chr5.2:31086348-31086367 None:intergenic 40.0%
ACACTAGGAATCAAGAACGG+AGG + chr5.2:31087033-31087052 MS.gene55256:CDS 45.0%
ACTTCTGCAAAAACTGCCAG+AGG + chr5.2:31086542-31086561 MS.gene55256:CDS 45.0%
AGAAGTTTCTGTTTCGGACG+AGG - chr5.2:31086371-31086390 None:intergenic 45.0%
ATGATTCTTGACTCCCCCTA+TGG - chr5.2:31087082-31087101 None:intergenic 45.0%
ATGGGATTTGCATCCATAGG+GGG + chr5.2:31087066-31087085 MS.gene55256:CDS 45.0%
ATGTTCCTCCACAGTTTCCT+TGG + chr5.2:31086734-31086753 MS.gene55256:CDS 45.0%
CATGGGATTTGCATCCATAG+GGG + chr5.2:31087065-31087084 MS.gene55256:CDS 45.0%
GAAGTTTCTGTTTCGGACGA+GGG - chr5.2:31086370-31086389 None:intergenic 45.0%
GACACAGGATAACACATTGC+AGG - chr5.2:31086763-31086782 None:intergenic 45.0%
GGACAAGGAACAATTAGGTC+TGG - chr5.2:31086457-31086476 None:intergenic 45.0%
GTTGGAGAATTCACTTCAGG+TGG - chr5.2:31085371-31085390 None:intergenic 45.0%
TAACACATTGCAGGGATCCA+AGG - chr5.2:31086754-31086773 None:intergenic 45.0%
TCGGTTGGAGAATTCACTTC+AGG - chr5.2:31085374-31085393 None:intergenic 45.0%
TGAGGCCTCATTCCAAACTA+CGG + chr5.2:31086294-31086313 MS.gene55256:CDS 45.0%
TGGAGAATTCACTTCAGGTG+GGG - chr5.2:31085369-31085388 None:intergenic 45.0%
! AACGGAGGAGAGCTTTTCAT+GGG + chr5.2:31087048-31087067 MS.gene55256:CDS 45.0%
! ATCCTCCGTAGTTTGGAATG+AGG - chr5.2:31086302-31086321 None:intergenic 45.0%
! GCAAAAACTGCCAGAGGTAT+TGG + chr5.2:31086548-31086567 MS.gene55256:CDS 45.0%
! GTGTCCATGCTTTTGCATCT+TGG - chr5.2:31086478-31086497 None:intergenic 45.0%
!! CACATCGATTCAAGAGGATG+AGG + chr5.2:31086276-31086295 MS.gene55256:CDS 45.0%
!! GGCAGTTTTTGCAGAAGTGT+CGG - chr5.2:31086540-31086559 None:intergenic 45.0%
!!! CAGTTTTTGCAGAAGTGTCG+GGG - chr5.2:31086538-31086557 None:intergenic 45.0%
!!! GCAGTTTTTGCAGAAGTGTC+GGG - chr5.2:31086539-31086558 None:intergenic 45.0%
CATATTTCTCGTGGCTCCTC+CGG - chr5.2:31086578-31086597 None:intergenic 50.0%
GGTCTCTTGTGTTCCATGAG+AGG + chr5.2:31087151-31087170 MS.gene55256:CDS 50.0%
GTGGTTGTTTGGCACCATCA+TGG + chr5.2:31086809-31086828 MS.gene55256:CDS 50.0%
TGAGTTCTTGTGCCATGCCA+TGG + chr5.2:31085419-31085438 MS.gene55256:intron 50.0%
! GAACGGAGGAGAGCTTTTCA+TGG + chr5.2:31087047-31087066 MS.gene55256:CDS 50.0%
! TTTCGGACGAGGGAGTTTTC+AGG - chr5.2:31086360-31086379 None:intergenic 50.0%
!! TGCCAGAGGTATTGGACTTC+CGG + chr5.2:31086556-31086575 MS.gene55256:CDS 50.0%
CTCCGGAAGTCCAATACCTC+TGG - chr5.2:31086561-31086580 None:intergenic 55.0%
GCAGGGATCCAAGGAAACTG+TGG - chr5.2:31086745-31086764 None:intergenic 55.0%
GGCCTCATTCCAAACTACGG+AGG + chr5.2:31086297-31086316 MS.gene55256:CDS 55.0%
GGGATCCAAGGAAACTGTGG+AGG - chr5.2:31086742-31086761 None:intergenic 55.0%
GTTCTTGTGCCATGCCATGG+TGG + chr5.2:31085422-31085441 MS.gene55256:intron 55.0%
TCAGTGGCCTGAAGTCCTTC+TGG + chr5.2:31085863-31085882 MS.gene55256:intron 55.0%
!! CAGAGGTATTGGACTTCCGG+AGG + chr5.2:31086559-31086578 MS.gene55256:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 31085209 31087179 31085209 ID=MS.gene55256
chr5.2 mRNA 31085209 31087179 31085209 ID=MS.gene55256.t1;Parent=MS.gene55256
chr5.2 exon 31085209 31085415 31085209 ID=MS.gene55256.t1.exon1;Parent=MS.gene55256.t1
chr5.2 CDS 31085209 31085415 31085209 ID=cds.MS.gene55256.t1;Parent=MS.gene55256.t1
chr5.2 exon 31086256 31087179 31086256 ID=MS.gene55256.t1.exon2;Parent=MS.gene55256.t1
chr5.2 CDS 31086256 31087179 31086256 ID=cds.MS.gene55256.t1;Parent=MS.gene55256.t1
Gene Sequence

>MS.gene55256

ATGACTAATAATTCAACCATAAAGCTTTTTGGGAGAACAATTTTTCAAACATACAACACAGATGTCTCTACCAATGATTCTTCCTTAGAGTTAGAGTTTGGTTCTCCTTTTCCTCATGAAAATTCCTTTGATCATAGTCCTTATTCATCTTCATGTTCCCCACCTGAAGTGAATTCTCCAACCGAAAATGATGGAAAGAGATATAAGGTATGAGTTCTTGTGCCATGCCATGGTGGTTCTATTTATTGGATACATTTATTATTTAAAATTATGTTGCTAACTAGTGTGTTTTGAAATTATAACAGTGCAACTTTTTTCAGAAATTAGAAATTGTTAATGGGTTGATACAAATTTTATTTTGAGAAAAATTGCTTCTAGTTAAGACTTAGGAGTATATAATTATAATTAAGTGTCAGCAATTTTTCGTTGCTGTTTAGACCACTTTTTGGTTAGTTTTCATGATGATAGATGGTTGATGAGATTGTTATCTTTTCTCATTAGTAGATTTATTTAACCGAAATCGTCAATAAATCATGATCAATCATATGTATATCCGTAAAACCGGTTTAATCACAATGAATAACACGACTCAATCATGATTAAAGTCAATTATTGTTAATGATCTTACATGGTTTTATACAAACTTCATCTTAATCAGTGGCCTGAAGTCCTTCTGGATAAAAGTTCATTCAAGAATTTCGTGTTCTAAGACATGAGATTGCAAACTGAAATAAATTAAAATATCAAGAAGGACCATATAATATTCTGTTGTATATTTATGTCACATCGAATAAATCAGTTAGTCGAAGTCTAATGTAACACCAATACTTTAGATTAAATGTGTGTTTGATGTCCGACACGACACTAATACATGTACTTACAGTCAATATTTCACGCAGTTCTCAAATTAATATCGGTGGGAGTGTTGTATTCGATCCCGTGTCTATGTCAATACTTCATAGCTTTGCTTTATAATGTCAATTCATAAATCAGTATAATTGTGTTGCTCGAATTTTATTCAATCTAACCGCTATATACCAATTACAGGAAACATCAAGGAAAGAACTCACATCGATTCAAGAGGATGAGGCCTCATTCCAAACTACGGAGGATTCAAAATCTCACACATCTTCAAGCCTTGTTGAGAACCTGAAAACTCCCTCGTCCGAAACAGAAACTTCTGAACTGAATTCCCATAAAATTGATGAACAAAGTGATATGTCACAAGAGAAATCTCCAATGAAACCAGACCTAATTGTTCCTTGTCCAAGATGCAAAAGCATGGACACAAAATTTTGTTACTATAACAACTACAACGTTAAACAGCCCCGACACTTCTGCAAAAACTGCCAGAGGTATTGGACTTCCGGAGGAGCCACGAGAAATATGATTGTAGGTGCAGGTCGTCGCAAGAACAAACTCAATGCAGCCAATGGATTGCGTAGTACTACTATCTTGACCTTTGGATCAGATTCTCCTATTACGTCTTCAACTTCACTTGACAAGAAAGTGAATGTTGGATCACATGTTCCTCCACAGTTTCCTTGGATCCCTGCAATGTGTTATCCTGTGTCATTCTATCCCAATATAGCATACTATGGTGGTTGTTTGGCACCATCATGGAATGTACAATCTATCTCAACACAATCTTGTGGTCAAAATTCAACTACAGTAGGGAAACATTCAAGAGATGGGAACATTCTCGTTCATTCCAACTCAGAGACACAAAACCTTGGACCAGAAAGAAATAATAATAGTGTCTTGATCCCAAAGACACTGAGAATTGATGACCCTAACAAAATTGCGAAGAGTTCTATATGGTCAACACTAGGAATCAAGAACGGAGGAGAGCTTTTCATGGGATTTGCATCCATAGGGGGAGTCAAGAATCATGTTGAAACATCATCATCAGTTTTGCAAGCTAATCCTGTAGCTTTATCAAGGTCTCTTGTGTTCCATGAGAGGATTTGA
Protein sequence

>MS.gene55256.t1

MTNNSTIKLFGRTIFQTYNTDVSTNDSSLELEFGSPFPHENSFDHSPYSSSCSPPEVNSPTENDGKRYKETSRKELTSIQEDEASFQTTEDSKSHTSSSLVENLKTPSSETETSELNSHKIDEQSDMSQEKSPMKPDLIVPCPRCKSMDTKFCYYNNYNVKQPRHFCKNCQRYWTSGGATRNMIVGAGRRKNKLNAANGLRSTTILTFGSDSPITSSTSLDKKVNVGSHVPPQFPWIPAMCYPVSFYPNIAYYGGCLAPSWNVQSISTQSCGQNSTTVGKHSRDGNILVHSNSETQNLGPERNNNSVLIPKTLRIDDPNKIAKSSIWSTLGIKNGGELFMGFASIGGVKNHVETSSSVLQANPVALSRSLVFHERI