AlfalfaGEDB | MS.gene55378

M. sativa cultivar XinJiangDaYe / MS.gene55378

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55378.t1	XP_024639602.1	96.5	144	5	0	1	144	64	207	4.50E-70	273.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55378.t1	Q9SZL8	89.6	144	14	1	1	144	77	219	8.0e-68	257.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55378.t1	A0A396HY09	96.5	144	5	0	1	144	64	207	3.2e-70	273.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene55378.t1	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55378.t1	MTR_3g006370	50.485	103	48	2	1	103	54	153	1.81e-25	97.1
MS.gene55378.t1	MTR_4g067290	51.456	103	45	3	2	104	61	158	1.20e-24	95.1
MS.gene55378.t1	MTR_4g067290	51.456	103	45	3	2	104	61	158	1.28e-24	95.1
MS.gene55378.t1	MTR_4g067290	51.456	103	45	3	2	104	81	178	1.52e-24	95.1
MS.gene55378.t1	MTR_4g067290	51.456	103	45	3	2	104	81	178	1.54e-24	95.1
MS.gene55378.t1	MTR_4g067290	51.456	103	45	3	2	104	79	176	1.71e-24	95.1
MS.gene55378.t1	MTR_4g067290	51.456	103	45	3	2	104	121	218	3.12e-24	95.1
MS.gene55378.t1	MTR_2g098880	42.593	108	60	2	1	108	65	170	5.22e-24	93.6
MS.gene55378.t1	MTR_2g098880	42.593	108	60	2	1	108	77	182	6.23e-24	94.0
MS.gene55378.t1	MTR_2g098920	52.427	103	44	3	2	104	85	182	9.20e-24	93.2
MS.gene55378.t1	MTR_0402s0040	37.037	135	81	2	1	135	30	160	4.84e-23	94.0
MS.gene55378.t1	MTR_1g070355	34.483	145	93	2	2	146	79	221	3.04e-22	92.0
MS.gene55378.t1	MTR_5g015480	36.486	148	83	4	1	140	60	204	6.85e-19	80.5
MS.gene55378.t1	MTR_1g019850	32.331	133	80	2	1	133	74	196	3.74e-17	75.5
MS.gene55378.t1	MTR_7g028855	35.252	139	88	2	1	139	124	260	4.24e-17	77.0
MS.gene55378.t1	MTR_0853s0010	29.927	137	85	2	1	133	29	158	2.05e-16	72.0
MS.gene55378.t1	MTR_2g070590	35.294	102	63	1	1	102	21	119	2.51e-15	68.2
MS.gene55378.t1	MTR_4g035820	33.333	144	88	4	3	142	81	220	1.35e-14	70.1
MS.gene55378.t1	MTR_2g101460	39.496	119	66	3	3	119	61	175	1.92e-14	67.8
MS.gene55378.t1	MTR_2g101460	39.496	119	66	3	3	119	51	165	2.27e-14	67.4
MS.gene55378.t1	MTR_7g446520	34.965	143	73	3	1	143	92	214	5.16e-14	67.8
MS.gene55378.t1	MTR_7g113010	35.897	117	58	3	1	117	461	560	8.04e-14	67.8
MS.gene55378.t1	MTR_5g076070	29.771	131	90	2	3	133	59	187	2.09e-13	65.9
MS.gene55378.t1	MTR_6g017200	34.737	95	59	1	1	95	62	153	2.71e-13	63.5
MS.gene55378.t1	MTR_6g051425	26.897	145	94	3	4	142	24	162	1.51e-12	63.9
MS.gene55378.t1	MTR_1g008290	35.802	81	47	2	64	144	15	90	2.62e-12	60.8
MS.gene55378.t1	MTR_1g057920	37.805	82	48	1	1	82	38	116	1.90e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55378.t1	AT4G38180	89.583	144	14	1	1	144	77	219	1.16e-77	245
MS.gene55378.t1	AT3G07500	50.962	104	47	2	1	104	37	136	2.65e-28	103
MS.gene55378.t1	AT3G59470	53.398	103	43	3	2	104	75	172	1.92e-27	102
MS.gene55378.t1	AT3G59470	53.398	103	43	3	2	104	75	172	1.92e-27	102
MS.gene55378.t1	AT3G59470	53.398	103	43	3	2	104	87	184	2.30e-27	102
MS.gene55378.t1	AT2G43280	48.649	111	52	3	3	111	28	135	4.90e-25	95.5
MS.gene55378.t1	AT1G76320	45.217	115	55	4	1	115	1	107	8.35e-22	90.9
MS.gene55378.t1	AT1G76320	45.217	115	55	4	1	115	1	107	8.35e-22	90.9
MS.gene55378.t1	AT1G76320	45.217	115	55	4	1	115	1	107	8.60e-22	90.9
MS.gene55378.t1	AT1G76320	44.828	116	56	4	1	116	1	108	1.19e-21	90.5
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	42	154	2.68e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	1	113	2.96e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	42	154	3.00e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	42	154	3.00e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	42	154	3.00e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	42	154	3.03e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	42	154	3.03e-21	89.4
MS.gene55378.t1	AT2G32250	40.833	120	64	3	1	120	1	113	3.07e-21	89.4
MS.gene55378.t1	AT2G43280	46.078	102	50	3	12	111	2	100	2.31e-20	82.4
MS.gene55378.t1	AT4G15090	35.878	131	79	3	1	131	55	180	9.90e-20	84.7
MS.gene55378.t1	AT4G15090	35.878	131	79	3	1	131	55	180	9.90e-20	84.7
MS.gene55378.t1	AT4G15090	35.878	131	79	3	1	131	55	180	9.90e-20	84.7
MS.gene55378.t1	AT4G15090	35.878	131	79	3	1	131	55	180	9.90e-20	84.7
MS.gene55378.t1	AT4G15090	35.878	131	79	3	1	131	55	180	9.90e-20	84.7
MS.gene55378.t1	AT3G22170	38.211	123	55	3	1	108	74	190	3.80e-19	83.2
MS.gene55378.t1	AT3G22170	38.211	123	55	3	1	108	74	190	3.80e-19	83.2
MS.gene55378.t1	AT4G12850	40.196	102	54	2	1	102	15	109	1.54e-17	74.3
MS.gene55378.t1	AT5G28530	37.063	143	83	4	3	142	61	199	2.30e-17	78.2
MS.gene55378.t1	AT5G28530	37.063	143	83	4	3	142	61	199	2.38e-17	77.8
MS.gene55378.t1	AT5G28530	37.063	143	83	4	3	142	61	199	2.49e-17	77.8
MS.gene55378.t1	AT5G28530	37.063	143	83	4	3	142	61	199	2.53e-17	77.8
MS.gene55378.t1	AT5G28530	37.063	143	83	4	3	142	61	199	2.53e-17	77.8
MS.gene55378.t1	AT4G12850	40.196	102	54	2	1	102	15	109	3.10e-17	73.6
MS.gene55378.t1	AT4G12850	40.196	102	54	2	1	102	15	109	5.68e-17	73.9
MS.gene55378.t1	AT5G18960	39.216	102	45	3	1	102	47	131	2.40e-15	72.4
MS.gene55378.t1	AT5G18960	36.538	104	49	2	1	104	215	301	2.46e-14	69.3
MS.gene55378.t1	AT5G18960	39.216	102	45	3	1	102	47	131	2.40e-15	72.4
MS.gene55378.t1	AT5G18960	36.538	104	49	2	1	104	215	301	2.46e-14	69.3
MS.gene55378.t1	AT3G06250	37.500	104	48	2	1	104	194	280	4.44e-15	71.6
MS.gene55378.t1	AT3G06250	38.235	102	46	3	1	102	32	116	6.10e-15	71.2
MS.gene55378.t1	AT3G06250	37.500	104	48	2	1	104	194	280	4.44e-15	71.6
MS.gene55378.t1	AT3G06250	38.235	102	46	3	1	102	32	116	6.10e-15	71.2
MS.gene55378.t1	AT3G06250	37.500	104	48	2	1	104	194	280	4.44e-15	71.6
MS.gene55378.t1	AT3G06250	38.235	102	46	3	1	102	32	116	6.10e-15	71.2
MS.gene55378.t1	AT1G10240	32.867	143	91	4	3	142	54	194	4.58e-15	71.6
MS.gene55378.t1	AT1G10240	32.867	143	91	4	3	142	54	194	4.58e-15	71.6
MS.gene55378.t1	AT4G19990	37.719	114	54	3	2	102	26	135	5.58e-15	71.2
MS.gene55378.t1	AT4G19990	37.719	114	54	3	2	102	26	135	5.58e-15	71.2
MS.gene55378.t1	AT4G19990	37.719	114	54	3	2	102	40	149	5.87e-15	71.2
MS.gene55378.t1	AT4G19990	37.719	114	54	3	2	102	26	135	6.05e-15	71.2
MS.gene55378.t1	AT4G19990	37.719	114	54	3	2	102	26	135	6.05e-15	71.2
MS.gene55378.t1	AT4G19990	37.719	114	54	3	2	102	26	135	6.33e-15	71.2
MS.gene55378.t1	AT2G27110	31.707	123	65	3	1	123	53	156	8.99e-13	65.1
MS.gene55378.t1	AT2G27110	31.707	123	65	3	1	123	53	156	8.99e-13	65.1
MS.gene55378.t1	AT1G52520	38.679	106	49	5	1	102	89	182	3.05e-12	63.2
MS.gene55378.t1	AT1G80010	36.111	108	53	4	1	104	99	194	4.50e-12	62.8

Find 37 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGTACCCACCGTCAAATTTC+GGG	0.215520	5.2:-33749741	MS.gene55378:CDS
TCGTACCCACCGTCAAATTT+CGG	0.222221	5.2:-33749742	MS.gene55378:CDS
CAGCCTGTAGAGTGTCAATT+AGG	0.261268	5.2:+33749706	None:intergenic
TCTGCCAAGTGGATCGTATC+TGG	0.285428	5.2:-33749819	MS.gene55378:CDS
CACTCTACAGGCTGCTGGTA+TGG	0.289290	5.2:-33749697	MS.gene55378:CDS
GCGTTTGATCTCTCTGTCTT+TGG	0.332960	5.2:+33749902	None:intergenic
AGCCTGTAGAGTGTCAATTA+GGG	0.418252	5.2:+33749707	None:intergenic
CGAAGGCAGTGAACCTGATC+GGG	0.450486	5.2:+33749762	None:intergenic
CGAACTTGTTCCACCCGATC+AGG	0.450679	5.2:-33749775	MS.gene55378:CDS
ACTCTACAGGCTGCTGGTAT+GGG	0.465336	5.2:-33749696	MS.gene55378:CDS
CCAACCCTAGTGACTGTTCG+TGG	0.474439	5.2:+33749879	None:intergenic
TTACATCATCATCATCGGCT+TGG	0.483630	5.2:+33749642	None:intergenic
AATTCCTATGCTCGTTGTGT+TGG	0.484218	5.2:-33750035	MS.gene55378:CDS
TTGCAGATCCCGAAATTTGA+CGG	0.485726	5.2:+33749733	None:intergenic
CGAGTCTGAAGAGGCTGCTA+AGG	0.500073	5.2:-33750066	MS.gene55378:CDS
AACCCTAATTGACACTCTAC+AGG	0.506142	5.2:-33749709	MS.gene55378:CDS
GAGACAATTCGTTTGTGCTA+AGG	0.519061	5.2:-33749955	MS.gene55378:CDS
CAATTCGTTTGTGCTAAGGA+AGG	0.537258	5.2:-33749951	MS.gene55378:CDS
ACGAAGGCAGTGAACCTGAT+CGG	0.540515	5.2:+33749761	None:intergenic
TCTCGTCGTTCTCGCCGCTA+CGG	0.548020	5.2:-33749990	MS.gene55378:CDS
ATTGACACTCTACAGGCTGC+TGG	0.555898	5.2:-33749702	MS.gene55378:CDS
AATTCGTTTGTGCTAAGGAA+GGG	0.559501	5.2:-33749950	MS.gene55378:CDS
GAAACCAACACAACGAGCAT+AGG	0.573090	5.2:+33750031	None:intergenic
AACGCCCACGAACAGTCACT+AGG	0.593060	5.2:-33749884	MS.gene55378:CDS
CCACGAACAGTCACTAGGGT+TGG	0.604393	5.2:-33749879	MS.gene55378:CDS
AGATCCCGAAATTTGACGGT+GGG	0.604477	5.2:+33749737	None:intergenic
AACGACGAGAAGAACTGACT+CGG	0.618808	5.2:+33750003	None:intergenic
AAATTTGACGGTGGGTACGA+AGG	0.620020	5.2:+33749745	None:intergenic
ACGACGAGAAGAACTGACTC+GGG	0.622470	5.2:+33750004	None:intergenic
TATGGAATTCGAGTCTGAAG+AGG	0.624053	5.2:-33750075	MS.gene55378:CDS
CAACCCTAGTGACTGTTCGT+GGG	0.628389	5.2:+33749880	None:intergenic
AGGCAGTGAACCTGATCGGG+TGG	0.632274	5.2:+33749765	None:intergenic
ACGCCCACGAACAGTCACTA+GGG	0.639492	5.2:-33749883	MS.gene55378:CDS
CAGATCCCGAAATTTGACGG+TGG	0.641830	5.2:+33749736	None:intergenic
GAAGCCAGATACGATCCACT+TGG	0.669193	5.2:+33749815	None:intergenic
TCTGAATGATAGCTCCGTAG+CGG	0.723190	5.2:+33749976	None:intergenic
AGTGCAGACATAATTCTGTG+AGG	0.753924	5.2:+33749672	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTCGTTTGTGCTAAGGAA+GGG	-	chr5.2:33749777-33749796	MS.gene55378:CDS	35.0%
!!	GTGCATTTTGACAGATAAAG+AGG	+	chr5.2:33749882-33749901	None:intergenic	35.0%
	AACCCTAATTGACACTCTAC+AGG	-	chr5.2:33750018-33750037	MS.gene55378:CDS	40.0%
	AATTCCTATGCTCGTTGTGT+TGG	-	chr5.2:33749692-33749711	MS.gene55378:CDS	40.0%
	AGTGCAGACATAATTCTGTG+AGG	+	chr5.2:33750058-33750077	None:intergenic	40.0%
	CAATTCGTTTGTGCTAAGGA+AGG	-	chr5.2:33749776-33749795	MS.gene55378:CDS	40.0%
	GAGACAATTCGTTTGTGCTA+AGG	-	chr5.2:33749772-33749791	MS.gene55378:CDS	40.0%
	TTGCAGATCCCGAAATTTGA+CGG	+	chr5.2:33749997-33750016	None:intergenic	40.0%
!	AGCCTGTAGAGTGTCAATTA+GGG	+	chr5.2:33750023-33750042	None:intergenic	40.0%
	AAATTTGACGGTGGGTACGA+AGG	+	chr5.2:33749985-33750004	None:intergenic	45.0%
	AACGACGAGAAGAACTGACT+CGG	+	chr5.2:33749727-33749746	None:intergenic	45.0%
	AGATCCCGAAATTTGACGGT+GGG	+	chr5.2:33749993-33750012	None:intergenic	45.0%
	GAAACCAACACAACGAGCAT+AGG	+	chr5.2:33749699-33749718	None:intergenic	45.0%
	GCGTTTGATCTCTCTGTCTT+TGG	+	chr5.2:33749828-33749847	None:intergenic	45.0%
	TCGTACCCACCGTCAAATTT+CGG	-	chr5.2:33749985-33750004	MS.gene55378:CDS	45.0%
	TCTGAATGATAGCTCCGTAG+CGG	+	chr5.2:33749754-33749773	None:intergenic	45.0%
!	CAGCCTGTAGAGTGTCAATT+AGG	+	chr5.2:33750024-33750043	None:intergenic	45.0%
!	TGCACGATTTTTCTGCCAAG+TGG	-	chr5.2:33749897-33749916	MS.gene55378:CDS	45.0%
	ACGAAGGCAGTGAACCTGAT+CGG	+	chr5.2:33749969-33749988	None:intergenic	50.0%
	ACTCTACAGGCTGCTGGTAT+GGG	-	chr5.2:33750031-33750050	MS.gene55378:CDS	50.0%
	ATTGACACTCTACAGGCTGC+TGG	-	chr5.2:33750025-33750044	MS.gene55378:CDS	50.0%
	CAACCCTAGTGACTGTTCGT+GGG	+	chr5.2:33749850-33749869	None:intergenic	50.0%
	CAGATCCCGAAATTTGACGG+TGG	+	chr5.2:33749994-33750013	None:intergenic	50.0%
	CGTACCCACCGTCAAATTTC+GGG	-	chr5.2:33749986-33750005	MS.gene55378:CDS	50.0%
	GAAGCCAGATACGATCCACT+TGG	+	chr5.2:33749915-33749934	None:intergenic	50.0%
	TCTGCCAAGTGGATCGTATC+TGG	-	chr5.2:33749908-33749927	MS.gene55378:CDS	50.0%
!	ACGACGAGAAGAACTGACTC+GGG	+	chr5.2:33749726-33749745	None:intergenic	50.0%
	AACGCCCACGAACAGTCACT+AGG	-	chr5.2:33749843-33749862	MS.gene55378:CDS	55.0%
	ACGCCCACGAACAGTCACTA+GGG	-	chr5.2:33749844-33749863	MS.gene55378:CDS	55.0%
	CACTCTACAGGCTGCTGGTA+TGG	-	chr5.2:33750030-33750049	MS.gene55378:CDS	55.0%
	CCAACCCTAGTGACTGTTCG+TGG	+	chr5.2:33749851-33749870	None:intergenic	55.0%
	CCACGAACAGTCACTAGGGT+TGG	-	chr5.2:33749848-33749867	MS.gene55378:CDS	55.0%
	CGAAGGCAGTGAACCTGATC+GGG	+	chr5.2:33749968-33749987	None:intergenic	55.0%
	CGAGTCTGAAGAGGCTGCTA+AGG	-	chr5.2:33749661-33749680	MS.gene55378:CDS	55.0%
!	CGAACTTGTTCCACCCGATC+AGG	-	chr5.2:33749952-33749971	MS.gene55378:CDS	55.0%
	AGGCAGTGAACCTGATCGGG+TGG	+	chr5.2:33749965-33749984	None:intergenic	60.0%
	TCTCGTCGTTCTCGCCGCTA+CGG	-	chr5.2:33749737-33749756	MS.gene55378:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	33749653	33750096	33749653	ID=MS.gene55378
chr5.2	mRNA	33749653	33750096	33749653	ID=MS.gene55378.t1;Parent=MS.gene55378
chr5.2	exon	33749653	33750096	33749653	ID=MS.gene55378.t1.exon1;Parent=MS.gene55378.t1
chr5.2	CDS	33749653	33750096	33749653	ID=cds.MS.gene55378.t1;Parent=MS.gene55378.t1

Gene Sequence

>MS.gene55378

ATGGAATTCGAGTCTGAAGAGGCTGCTAAGGCTTTCTATAATTCCTATGCTCGTTGTGTTGGTTTCAGTACCCGAGTCAGTTCTTCTCGTCGTTCTCGCCGCTACGGAGCTATCATTCAGAGACAATTCGTTTGTGCTAAGGAAGGGTTTCGCAATTTGAACGAGAAACGTACCAAAGACAGAGAGATCAAACGCCCACGAACAGTCACTAGGGTTGGATGCAAAGCCTCTTTATCTGTCAAAATGCACGATTTTTCTGCCAAGTGGATCGTATCTGGCTTCGTCAGAGAACACAATCACGAACTTGTTCCACCCGATCAGGTTCACTGCCTTCGTACCCACCGTCAAATTTCGGGATCTGCAAAAACCCTAATTGACACTCTACAGGCTGCTGGTATGGGTCCTCACAGAATTATGTCTGCACTCATCAAGCCAAGCCGATGA

Protein sequence

>MS.gene55378.t1

MEFESEEAAKAFYNSYARCVGFSTRVSSSRRSRRYGAIIQRQFVCAKEGFRNLNEKRTKDREIKRPRTVTRVGCKASLSVKMHDFSAKWIVSGFVREHNHELVPPDQVHCLRTHRQISGSAKTLIDTLQAAGMGPHRIMSALIKPSR