Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55721.t1 | XP_013447077.1 | 99.2 | 128 | 1 | 0 | 1 | 128 | 197 | 324 | 2.40E-64 | 254.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55721.t1 | Q940T9 | 46.2 | 52 | 28 | 0 | 25 | 76 | 291 | 342 | 9.9e-06 | 51.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55721.t1 | A0A072TU95 | 99.2 | 128 | 1 | 0 | 1 | 128 | 197 | 324 | 1.8e-64 | 254.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene55721.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55721.t1 | MTR_8g098725 | 99.219 | 128 | 1 | 0 | 1 | 128 | 201 | 328 | 3.50e-93 | 272 |
| MS.gene55721.t1 | MTR_8g098725 | 99.219 | 128 | 1 | 0 | 1 | 128 | 197 | 324 | 3.72e-93 | 271 |
| MS.gene55721.t1 | MTR_5g010120 | 65.217 | 92 | 30 | 2 | 5 | 95 | 150 | 240 | 4.33e-33 | 116 |
| MS.gene55721.t1 | MTR_2g068730 | 77.419 | 62 | 14 | 0 | 15 | 76 | 149 | 210 | 5.70e-25 | 95.9 |
| MS.gene55721.t1 | MTR_1g073350 | 64.706 | 68 | 23 | 1 | 6 | 73 | 294 | 360 | 3.60e-24 | 95.9 |
| MS.gene55721.t1 | MTR_1g044785 | 46.429 | 112 | 53 | 3 | 6 | 117 | 112 | 216 | 2.99e-23 | 90.5 |
| MS.gene55721.t1 | MTR_2g096080 | 51.685 | 89 | 34 | 2 | 1 | 83 | 121 | 206 | 2.36e-21 | 85.9 |
| MS.gene55721.t1 | MTR_4g061823 | 60.606 | 66 | 25 | 1 | 9 | 73 | 124 | 189 | 1.05e-20 | 84.3 |
| MS.gene55721.t1 | MTR_4g061910 | 60.606 | 66 | 25 | 1 | 9 | 73 | 124 | 189 | 1.05e-20 | 84.3 |
| MS.gene55721.t1 | MTR_1g008220 | 52.174 | 69 | 31 | 1 | 5 | 73 | 210 | 276 | 4.79e-18 | 77.8 |
| MS.gene55721.t1 | MTR_3g091340 | 60.345 | 58 | 23 | 0 | 16 | 73 | 188 | 245 | 5.57e-18 | 77.0 |
| MS.gene55721.t1 | MTR_3g091340 | 60.345 | 58 | 23 | 0 | 16 | 73 | 181 | 238 | 6.14e-18 | 77.0 |
| MS.gene55721.t1 | MTR_3g091340 | 60.345 | 58 | 23 | 0 | 16 | 73 | 217 | 274 | 1.17e-17 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55721.t1 | AT1G63820 | 58.197 | 122 | 39 | 2 | 7 | 116 | 170 | 291 | 8.89e-37 | 127 |
| MS.gene55721.t1 | AT5G41380 | 60.870 | 115 | 32 | 4 | 2 | 113 | 192 | 296 | 4.53e-36 | 125 |
| MS.gene55721.t1 | AT3G12890 | 67.647 | 68 | 22 | 0 | 9 | 76 | 119 | 186 | 5.29e-25 | 95.5 |
| MS.gene55721.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 6 | 73 | 295 | 363 | 1.73e-23 | 94.0 |
| MS.gene55721.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 6 | 73 | 286 | 354 | 3.14e-23 | 93.2 |
| MS.gene55721.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 6 | 73 | 275 | 343 | 3.65e-23 | 93.2 |
| MS.gene55721.t1 | AT2G33350 | 63.768 | 69 | 24 | 1 | 6 | 73 | 287 | 355 | 3.68e-23 | 93.2 |
| MS.gene55721.t1 | AT1G04500 | 68.254 | 63 | 20 | 0 | 20 | 82 | 279 | 341 | 5.52e-23 | 92.4 |
| MS.gene55721.t1 | AT1G04500 | 68.254 | 63 | 20 | 0 | 20 | 82 | 279 | 341 | 5.52e-23 | 92.4 |
| MS.gene55721.t1 | AT1G04500 | 68.254 | 63 | 20 | 0 | 20 | 82 | 270 | 332 | 3.54e-22 | 90.1 |
| MS.gene55721.t1 | AT5G59990 | 51.042 | 96 | 47 | 0 | 2 | 97 | 136 | 231 | 3.90e-18 | 77.4 |
| MS.gene55721.t1 | AT4G27900 | 49.206 | 63 | 32 | 0 | 11 | 73 | 196 | 258 | 1.17e-16 | 73.9 |
| MS.gene55721.t1 | AT4G27900 | 49.206 | 63 | 32 | 0 | 11 | 73 | 196 | 258 | 1.17e-16 | 73.9 |
| MS.gene55721.t1 | AT5G53420 | 59.184 | 49 | 20 | 0 | 25 | 73 | 135 | 183 | 5.82e-16 | 70.5 |
| MS.gene55721.t1 | AT5G53420 | 59.184 | 49 | 20 | 0 | 25 | 73 | 135 | 183 | 5.82e-16 | 70.5 |
| MS.gene55721.t1 | AT5G53420 | 59.184 | 49 | 20 | 0 | 25 | 73 | 135 | 183 | 5.82e-16 | 70.5 |
| MS.gene55721.t1 | AT5G53420 | 49.206 | 63 | 32 | 0 | 11 | 73 | 200 | 262 | 1.18e-15 | 71.2 |
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGAAGACGAAGTCGATTTC+TGG | 0.227779 | 4.2:+5967014 | MS.gene55721:CDS |
| GAACTTTAACAAGACTATTA+AGG | 0.296751 | 4.2:+5966811 | MS.gene55721:CDS |
| TGTTCTCTTGGCTCTGTACT+TGG | 0.332786 | 4.2:-5966783 | None:intergenic |
| AAAGTTCCTCTGTGTTCTCT+TGG | 0.348760 | 4.2:-5966795 | None:intergenic |
| GTTGGTCAATTTCAGTACTT+TGG | 0.368744 | 4.2:+5967478 | MS.gene55721:CDS |
| CTGATTAAACATGGAGCCTT+AGG | 0.397252 | 4.2:-5966989 | None:intergenic |
| TTGGTCAATTTCAGTACTTT+GGG | 0.401778 | 4.2:+5967479 | MS.gene55721:CDS |
| TCTTGCAAATTCCCCTCCGT+TGG | 0.447340 | 4.2:-5966704 | None:intergenic |
| TGAAGACGAAGTCGATTTCT+GGG | 0.449240 | 4.2:+5967015 | MS.gene55721:CDS |
| AAGAATAGTGGTTACGGAGT+TGG | 0.483748 | 4.2:+5967460 | MS.gene55721:CDS |
| GAACAATATCTGAAGAATAG+TGG | 0.497442 | 4.2:+5967448 | MS.gene55721:CDS |
| GAGGAACAAAATTTCAAAGT+AGG | 0.499993 | 4.2:+5966725 | MS.gene55721:CDS |
| ATCGACCACGCGTACGTGGC+AGG | 0.512738 | 4.2:+5966936 | MS.gene55721:CDS |
| TATCTGAAGAATAGTGGTTA+CGG | 0.515829 | 4.2:+5967454 | MS.gene55721:CDS |
| AATAATATGTCTCCAACGGA+GGG | 0.534384 | 4.2:+5966692 | MS.gene55721:CDS |
| AAATAATATGTCTCCAACGG+AGG | 0.560154 | 4.2:+5966691 | MS.gene55721:CDS |
| AGAAAATAATATGTCTCCAA+CGG | 0.563673 | 4.2:+5966688 | MS.gene55721:CDS |
| CGAGCCCACGGAGATTCCTA+AGG | 0.567771 | 4.2:+5966973 | MS.gene55721:CDS |
| TCTTCATCTCTGATTAAACA+TGG | 0.570013 | 4.2:-5966998 | None:intergenic |
| TGCACGCAATGACGAGCCCA+CGG | 0.603558 | 4.2:+5966961 | MS.gene55721:CDS |
| GCAAACCTGCCACGTACGCG+TGG | 0.603581 | 4.2:-5966941 | None:intergenic |
| GACAATCGACCACGCGTACG+TGG | 0.616500 | 4.2:+5966932 | MS.gene55721:CDS |
| TGATGTAGATGGAAGAATTG+CGG | 0.651165 | 4.2:+5967392 | MS.gene55721:intron |
| ATAATATGTCTCCAACGGAG+GGG | 0.654626 | 4.2:+5966693 | MS.gene55721:CDS |
| CAAAGTAGGTCGTTATAGCG+CGG | 0.657198 | 4.2:+5966739 | MS.gene55721:CDS |
| AACGGAGGGGAATTTGCAAG+AGG | 0.665906 | 4.2:+5966706 | MS.gene55721:CDS |
| CATGAAGATGATGTAACAGT+GGG | 0.675111 | 4.2:+5967418 | MS.gene55721:CDS |
| GGAGCCTTAGGAATCTCCGT+GGG | 0.684622 | 4.2:-5966977 | None:intergenic |
| GCATGAAGATGATGTAACAG+TGG | 0.695685 | 4.2:+5967417 | MS.gene55721:CDS |
| TGGAGCCTTAGGAATCTCCG+TGG | 0.702219 | 4.2:-5966978 | None:intergenic |
| ACAGAGCCAAGAGAACACAG+AGG | 0.782868 | 4.2:+5966789 | MS.gene55721:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAAAATAATATGTCTCCAA+CGG | + | chr4.2:5966688-5966707 | MS.gene55721:CDS | 25.0% |
| ! | GAACTTTAACAAGACTATTA+AGG | + | chr4.2:5966811-5966830 | MS.gene55721:CDS | 25.0% |
| !! | TTTGAAATAGTAAGCATGTA+AGG | + | chr4.2:5967101-5967120 | MS.gene55721:intron | 25.0% |
| GAACAATATCTGAAGAATAG+TGG | + | chr4.2:5967448-5967467 | MS.gene55721:CDS | 30.0% | |
| GAGGAACAAAATTTCAAAGT+AGG | + | chr4.2:5966725-5966744 | MS.gene55721:CDS | 30.0% | |
| TATCTGAAGAATAGTGGTTA+CGG | + | chr4.2:5967454-5967473 | MS.gene55721:CDS | 30.0% | |
| TCTTCATCTCTGATTAAACA+TGG | - | chr4.2:5967001-5967020 | None:intergenic | 30.0% | |
| TTGGTCAATTTCAGTACTTT+GGG | + | chr4.2:5967479-5967498 | MS.gene55721:CDS | 30.0% | |
| ! | GATTTTACTTGTACAAGCTA+TGG | - | chr4.2:5967160-5967179 | None:intergenic | 30.0% |
| !! | GACTATTGATTGAGTTGAAA+GGG | + | chr4.2:5967349-5967368 | MS.gene55721:intron | 30.0% |
| !! | TGACTATTGATTGAGTTGAA+AGG | + | chr4.2:5967348-5967367 | MS.gene55721:intron | 30.0% |
| !!! | AAAGGGTTTTGATTCTTATG+TGG | + | chr4.2:5967366-5967385 | MS.gene55721:intron | 30.0% |
| AAATAATATGTCTCCAACGG+AGG | + | chr4.2:5966691-5966710 | MS.gene55721:CDS | 35.0% | |
| AATAATATGTCTCCAACGGA+GGG | + | chr4.2:5966692-5966711 | MS.gene55721:CDS | 35.0% | |
| CATGAAGATGATGTAACAGT+GGG | + | chr4.2:5967418-5967437 | MS.gene55721:CDS | 35.0% | |
| GCATGTAAGGATTGTACATT+TGG | + | chr4.2:5967114-5967133 | MS.gene55721:intron | 35.0% | |
| GTTGGTCAATTTCAGTACTT+TGG | + | chr4.2:5967478-5967497 | MS.gene55721:CDS | 35.0% | |
| TGATGTAGATGGAAGAATTG+CGG | + | chr4.2:5967392-5967411 | MS.gene55721:intron | 35.0% | |
| !! | TTATGTGGCTTTGATGTAGA+TGG | + | chr4.2:5967381-5967400 | MS.gene55721:intron | 35.0% |
| AAAGTTCCTCTGTGTTCTCT+TGG | - | chr4.2:5966798-5966817 | None:intergenic | 40.0% | |
| AAGAATAGTGGTTACGGAGT+TGG | + | chr4.2:5967460-5967479 | MS.gene55721:CDS | 40.0% | |
| ATAATATGTCTCCAACGGAG+GGG | + | chr4.2:5966693-5966712 | MS.gene55721:CDS | 40.0% | |
| CTGATTAAACATGGAGCCTT+AGG | - | chr4.2:5966992-5967011 | None:intergenic | 40.0% | |
| GCATGAAGATGATGTAACAG+TGG | + | chr4.2:5967417-5967436 | MS.gene55721:CDS | 40.0% | |
| ! | ATGAAGACGAAGTCGATTTC+TGG | + | chr4.2:5967014-5967033 | MS.gene55721:CDS | 40.0% |
| ! | TGAAGACGAAGTCGATTTCT+GGG | + | chr4.2:5967015-5967034 | MS.gene55721:CDS | 40.0% |
| CAAAGTAGGTCGTTATAGCG+CGG | + | chr4.2:5966739-5966758 | MS.gene55721:CDS | 45.0% | |
| !! | TGTTCTCTTGGCTCTGTACT+TGG | - | chr4.2:5966786-5966805 | None:intergenic | 45.0% |
| AACGGAGGGGAATTTGCAAG+AGG | + | chr4.2:5966706-5966725 | MS.gene55721:CDS | 50.0% | |
| ACAGAGCCAAGAGAACACAG+AGG | + | chr4.2:5966789-5966808 | MS.gene55721:CDS | 50.0% | |
| TCTTGCAAATTCCCCTCCGT+TGG | - | chr4.2:5966707-5966726 | None:intergenic | 50.0% | |
| GGAGCCTTAGGAATCTCCGT+GGG | - | chr4.2:5966980-5966999 | None:intergenic | 55.0% | |
| TGGAGCCTTAGGAATCTCCG+TGG | - | chr4.2:5966981-5967000 | None:intergenic | 55.0% | |
| CGAGCCCACGGAGATTCCTA+AGG | + | chr4.2:5966973-5966992 | MS.gene55721:CDS | 60.0% | |
| GACAATCGACCACGCGTACG+TGG | + | chr4.2:5966932-5966951 | MS.gene55721:CDS | 60.0% | |
| TGCACGCAATGACGAGCCCA+CGG | + | chr4.2:5966961-5966980 | MS.gene55721:CDS | 60.0% | |
| ATCGACCACGCGTACGTGGC+AGG | + | chr4.2:5966936-5966955 | MS.gene55721:CDS | 65.0% | |
| GCAAACCTGCCACGTACGCG+TGG | - | chr4.2:5966944-5966963 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 5966683 | 5967507 | 5966683 | ID=MS.gene55721 |
| chr4.2 | mRNA | 5966683 | 5967507 | 5966683 | ID=MS.gene55721.t1;Parent=MS.gene55721 |
| chr4.2 | exon | 5966683 | 5966832 | 5966683 | ID=MS.gene55721.t1.exon1;Parent=MS.gene55721.t1 |
| chr4.2 | CDS | 5966683 | 5966832 | 5966683 | ID=cds.MS.gene55721.t1;Parent=MS.gene55721.t1 |
| chr4.2 | exon | 5966908 | 5967036 | 5966908 | ID=MS.gene55721.t1.exon2;Parent=MS.gene55721.t1 |
| chr4.2 | CDS | 5966908 | 5967036 | 5966908 | ID=cds.MS.gene55721.t1;Parent=MS.gene55721.t1 |
| chr4.2 | exon | 5967400 | 5967507 | 5967400 | ID=MS.gene55721.t1.exon3;Parent=MS.gene55721.t1 |
| chr4.2 | CDS | 5967400 | 5967507 | 5967400 | ID=cds.MS.gene55721.t1;Parent=MS.gene55721.t1 |
| Gene Sequence |
| Protein sequence |
