AlfalfaGEDB | MS.gene56077

M. sativa cultivar XinJiangDaYe / MS.gene56077

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56077.t1	KEH30840.1	72.2	126	6	1	1	97	51	176	7.80E-39	169.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56077.t1	Q6X7K0	74.3	74	17	1	4	75	64	137	6.1e-24	111.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56077.t1	A0A072UYB2	72.2	126	6	1	1	97	51	176	5.6e-39	169.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene56077.t1	TF	HB-WOX

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56077.t1	MTR_4g084550	75.397	126	2	1	1	97	51	176	1.15e-61	186
MS.gene56077.t1	MTR_8g107210	82.812	64	11	0	12	75	94	157	1.26e-32	116
MS.gene56077.t1	MTR_7g060630	71.642	67	19	0	7	73	1	67	2.75e-30	106
MS.gene56077.t1	MTR_5g021930	65.672	67	22	1	10	75	26	92	8.75e-27	100
MS.gene56077.t1	MTR_1g019130	57.317	82	34	1	13	93	89	170	1.26e-26	97.8
MS.gene56077.t1	MTR_1g019130	57.317	82	34	1	13	93	89	170	2.41e-26	97.4
MS.gene56077.t1	MTR_4g063735	67.742	62	20	0	14	75	25	86	4.98e-26	97.1
MS.gene56077.t1	MTR_5g081990	66.129	62	21	0	14	75	33	94	5.49e-26	95.1
MS.gene56077.t1	MTR_8g095580	80.769	52	10	0	24	75	92	143	3.42e-25	92.8
MS.gene56077.t1	MTR_3g088485	82.353	51	9	0	25	75	91	141	3.51e-25	92.4
MS.gene56077.t1	MTR_1g115315	53.846	65	30	0	14	78	85	149	7.50e-20	80.9
MS.gene56077.t1	MTR_1g115315	53.846	65	30	0	14	78	85	149	1.09e-19	80.5
MS.gene56077.t1	MTR_7g025010	46.269	67	36	0	9	75	42	108	7.67e-18	77.0
MS.gene56077.t1	MTR_2g015000	44.615	65	36	0	9	73	50	114	7.12e-17	74.3
MS.gene56077.t1	MTR_4g088070	44.776	67	37	0	8	74	23	89	1.28e-16	73.6
MS.gene56077.t1	MTR_4g088080	38.356	73	45	0	2	74	28	100	3.58e-14	66.2
MS.gene56077.t1	MTR_3g115620	41.270	63	37	0	14	76	82	144	8.57e-14	64.3
MS.gene56077.t1	MTR_7g023810	43.750	64	36	0	14	77	68	131	1.91e-13	62.8
MS.gene56077.t1	MTR_6g027390	45.283	53	29	0	13	65	31	83	1.62e-12	61.6
MS.gene56077.t1	MTR_7g086940	45.098	51	28	0	13	63	27	77	2.15e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56077.t1	AT3G18010	81.250	64	12	0	12	75	74	137	2.21e-32	115
MS.gene56077.t1	AT2G28610	67.164	67	22	0	7	73	1	67	1.40e-29	105
MS.gene56077.t1	AT5G59340	67.188	64	21	0	12	75	12	75	2.05e-27	100
MS.gene56077.t1	AT2G17950	62.857	70	25	1	10	78	34	103	4.08e-26	98.2
MS.gene56077.t1	AT2G01500	74.545	55	14	0	14	68	61	115	5.59e-26	97.4
MS.gene56077.t1	AT1G46480	63.235	68	24	1	8	75	85	151	2.82e-25	93.6
MS.gene56077.t1	AT3G11260	62.903	62	23	0	14	75	24	85	3.26e-25	93.2
MS.gene56077.t1	AT1G46480	63.235	68	24	1	8	75	85	151	1.38e-24	93.2
MS.gene56077.t1	AT5G05770	57.353	68	29	0	14	81	29	96	1.67e-23	87.0
MS.gene56077.t1	AT2G33880	44.615	65	36	0	9	73	50	114	1.01e-16	73.9
MS.gene56077.t1	AT2G33880	44.615	65	36	0	9	73	50	114	1.38e-16	73.6
MS.gene56077.t1	AT5G45980	44.615	65	36	0	9	73	50	114	3.12e-16	72.0
MS.gene56077.t1	AT4G35550	48.387	62	32	0	14	75	99	160	7.82e-16	70.5
MS.gene56077.t1	AT1G20700	45.000	60	33	0	14	73	33	92	1.20e-14	65.1
MS.gene56077.t1	AT1G20700	45.000	60	33	0	14	73	95	154	4.47e-14	64.7
MS.gene56077.t1	AT1G20700	45.000	60	33	0	14	73	95	154	5.47e-14	64.7
MS.gene56077.t1	AT3G03660	49.057	53	27	0	13	65	32	84	1.12e-13	64.7
MS.gene56077.t1	AT3G03660	49.057	53	27	0	13	65	32	84	1.65e-13	64.3
MS.gene56077.t1	AT3G03660	49.057	53	27	0	13	65	32	84	2.40e-13	63.9
MS.gene56077.t1	AT3G03660	49.057	53	27	0	13	65	32	84	6.71e-13	62.8
MS.gene56077.t1	AT5G17810	43.396	53	30	0	13	65	20	72	8.71e-12	59.3
MS.gene56077.t1	AT5G17810	43.396	53	30	0	13	65	20	72	8.71e-12	59.3
MS.gene56077.t1	AT5G17810	43.396	53	30	0	13	65	20	72	8.92e-12	59.3

Find 20 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTCTCTCGCCTTGTGATTC+TGG	0.194023	4.4:-28271101	None:intergenic
GCTTCGCAGCTTCGACAATT+TGG	0.206061	4.4:+28271045	MS.gene56077:CDS
CGACAATTTGGAAAGATTGA+AGG	0.396848	4.4:+28271057	MS.gene56077:CDS
GAAGCTGCGAAGCTATTTGT+TGG	0.428703	4.4:-28271035	None:intergenic
CTTGCCTATACAATTCTTCA+AGG	0.428836	4.4:-28270984	None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG	0.431151	4.4:+28270980	MS.gene56077:CDS
GTTGGACTCCACCTTGAACT+TGG	0.444756	4.4:-28270943	None:intergenic
TGCATCCTCAACCAAGTTCA+AGG	0.472729	4.4:+28270932	MS.gene56077:CDS
CTCCACCTTGAACTTGGTTG+AGG	0.502185	4.4:-28270937	None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG	0.503667	4.4:-28271018	None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG	0.506780	4.4:-28271764	None:intergenic
TCAAGGACTAGTAATTGAAC+TGG	0.509163	4.4:-28270967	None:intergenic
ACTAGTAATTGAACTGGTGT+TGG	0.537149	4.4:-28270961	None:intergenic
CTTGAAGAATTGTATAGGCA+AGG	0.540070	4.4:+28270985	MS.gene56077:CDS
TTGAAGAATTGTATAGGCAA+GGG	0.590766	4.4:+28270986	MS.gene56077:CDS
AAACTGCAGTGAACAAGCAG+AGG	0.632631	4.4:+28271771	MS.gene56077:CDS
AGTTAAAGAGACAAAGAAGT+GGG	0.667079	4.4:+28271738	MS.gene56077:CDS
CTATTGGTTCCAGAATCACA+AGG	0.685006	4.4:+28271092	MS.gene56077:CDS
GAGTTAAAGAGACAAAGAAG+TGG	0.689037	4.4:+28271737	MS.gene56077:CDS
ATCCTCAACCAAGTTCAAGG+TGG	0.762805	4.4:+28270935	MS.gene56077:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATTTTTTTCTTTCAAATA+TGG	-	chr4.4:28271564-28271583	None:intergenic	10.0%
!!!	ATTGCTATTTTTGAAATTTT+AGG	+	chr4.4:28271616-28271635	MS.gene56077:intron	15.0%
!!!	TTGTTGTTTATTCAATTTAA+TGG	+	chr4.4:28271585-28271604	MS.gene56077:intron	15.0%
!!	AAGAAAAAAAAATGACCAAA+TGG	-	chr4.4:28271425-28271444	None:intergenic	20.0%
!!	AGAAAAAAAAATGACCAAAT+GGG	-	chr4.4:28271424-28271443	None:intergenic	20.0%
!!	TCATGAGTGAAATATAATAT+AGG	-	chr4.4:28271301-28271320	None:intergenic	20.0%
!!!	AAAGATAAATAGCTTTTGTA+AGG	+	chr4.4:28271358-28271377	MS.gene56077:intron	20.0%
!!!	ACTATACTTTTAAAGATGTT+AGG	-	chr4.4:28271540-28271559	None:intergenic	20.0%
!!!	CTAAAATCTCTAAAATTGTA+GGG	-	chr4.4:28271457-28271476	None:intergenic	20.0%
!	AAATATCAATTTAGCCCATT+TGG	+	chr4.4:28271407-28271426	MS.gene56077:intron	25.0%
!	AAGGAAAAAATGTGTTCTAT+TGG	+	chr4.4:28271076-28271095	MS.gene56077:CDS	25.0%
!	ACAGCTAGATATTATCTTTA+GGG	+	chr4.4:28271243-28271262	MS.gene56077:intron	25.0%
!	TACAGCTAGATATTATCTTT+AGG	+	chr4.4:28271242-28271261	MS.gene56077:intron	25.0%
!	TCAATATGATATTCTTGCAT+AGG	+	chr4.4:28271509-28271528	MS.gene56077:intron	25.0%
!!	ATGTATATGAGTTGAATTTG+TGG	+	chr4.4:28271685-28271704	MS.gene56077:intron	25.0%
!!!	ATTATCTTTAGGGTTATTCA+AGG	+	chr4.4:28271253-28271272	MS.gene56077:intron	25.0%
!!!	CCTAAAATCTCTAAAATTGT+AGG	-	chr4.4:28271458-28271477	None:intergenic	25.0%
!!!	CCTACAATTTTAGAGATTTT+AGG	+	chr4.4:28271455-28271474	MS.gene56077:intron	25.0%
	ACACACACTTCAAATTGTAA+GGG	-	chr4.4:28271655-28271674	None:intergenic	30.0%
	AGTTAAAGAGACAAAGAAGT+GGG	+	chr4.4:28271738-28271757	MS.gene56077:CDS	30.0%
	TAGTCCTTGAAGAATTGTAT+AGG	+	chr4.4:28270980-28270999	MS.gene56077:CDS	30.0%
	TTGAAGAATTGTATAGGCAA+GGG	+	chr4.4:28270986-28271005	MS.gene56077:CDS	30.0%
!	AATAGCTTTTGTAAGGATCT+CGG	+	chr4.4:28271365-28271384	MS.gene56077:intron	30.0%
!	GTTATTCAAGGTTCTTTTAG+TGG	+	chr4.4:28271265-28271284	MS.gene56077:intron	30.0%
	ACTAGTAATTGAACTGGTGT+TGG	-	chr4.4:28270964-28270983	None:intergenic	35.0%
	CACACACACTTCAAATTGTA+AGG	-	chr4.4:28271656-28271675	None:intergenic	35.0%
	CGACAATTTGGAAAGATTGA+AGG	+	chr4.4:28271057-28271076	MS.gene56077:CDS	35.0%
	CTTGAAGAATTGTATAGGCA+AGG	+	chr4.4:28270985-28271004	MS.gene56077:CDS	35.0%
	CTTGCCTATACAATTCTTCA+AGG	-	chr4.4:28270987-28271006	None:intergenic	35.0%
	GAGTTAAAGAGACAAAGAAG+TGG	+	chr4.4:28271737-28271756	MS.gene56077:CDS	35.0%
!	AGAGAAAGAGAAGTACGTTA+TGG	+	chr4.4:28271189-28271208	MS.gene56077:intron	35.0%
!	GAGAAAGAGAAGTACGTTAT+GGG	+	chr4.4:28271190-28271209	MS.gene56077:intron	35.0%
!	TCAAGGACTAGTAATTGAAC+TGG	-	chr4.4:28270970-28270989	None:intergenic	35.0%
!	TGAGTTGAATTTGTGGTGAT+TGG	+	chr4.4:28271692-28271711	MS.gene56077:intron	35.0%
!!	GATTGGATTTTGAAAGAGAC+AGG	+	chr4.4:28271709-28271728	MS.gene56077:intron	35.0%
	AATTGTTTCCCTCCATCTCA+AGG	-	chr4.4:28271143-28271162	None:intergenic	40.0%
	TTGAGATGGAGGGAAACAAT+TGG	+	chr4.4:28271142-28271161	MS.gene56077:intron	40.0%
	TTGTTCACTGCAGTTTGAAG+TGG	-	chr4.4:28271767-28271786	None:intergenic	40.0%
!	TGTTGGATTTGTTCAGCTGA+TGG	-	chr4.4:28271021-28271040	None:intergenic	40.0%
!!	ATTTTGAAAGAGACAGGTGG+TGG	+	chr4.4:28271715-28271734	MS.gene56077:intron	40.0%
!!	CTATTGGTTCCAGAATCACA+AGG	+	chr4.4:28271092-28271111	MS.gene56077:CDS	40.0%
!!	TGGATTTTGAAAGAGACAGG+TGG	+	chr4.4:28271712-28271731	MS.gene56077:intron	40.0%
	ATCCTCAACCAAGTTCAAGG+TGG	+	chr4.4:28270935-28270954	MS.gene56077:CDS	45.0%
	GAAGCTGCGAAGCTATTTGT+TGG	-	chr4.4:28271038-28271057	None:intergenic	45.0%
	TCAGAAAAGCAAGAAGCTGC+AGG	+	chr4.4:28271217-28271236	MS.gene56077:intron	45.0%
	TGCATCCTCAACCAAGTTCA+AGG	+	chr4.4:28270932-28270951	MS.gene56077:CDS	45.0%
	CTCCACCTTGAACTTGGTTG+AGG	-	chr4.4:28270940-28270959	None:intergenic	50.0%
	GCTTCGCAGCTTCGACAATT+TGG	+	chr4.4:28271045-28271064	MS.gene56077:CDS	50.0%
	GTTGGACTCCACCTTGAACT+TGG	-	chr4.4:28270946-28270965	None:intergenic	50.0%
	CTGTCTCTCTCTCTCTCTGC+TGG	-	chr4.4:28271336-28271355	None:intergenic	55.0%
	GCTCTCTCGCCTTGTGATTC+TGG	-	chr4.4:28271104-28271123	None:intergenic	55.0%
	CGTCGTCGCCTTGAGATGGA+GGG	+	chr4.4:28271132-28271151	MS.gene56077:intron	60.0%
!!	GAAGCGTCGTCGCCTTGAGA+TGG	+	chr4.4:28271128-28271147	MS.gene56077:intron	60.0%
	GCGTCGTCGCCTTGAGATGG+AGG	+	chr4.4:28271131-28271150	MS.gene56077:intron	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	28270913	28271792	28270913	ID=MS.gene56077
chr4.4	mRNA	28270913	28271792	28270913	ID=MS.gene56077.t1;Parent=MS.gene56077
chr4.4	exon	28270913	28271135	28270913	ID=MS.gene56077.t1.exon1;Parent=MS.gene56077.t1
chr4.4	CDS	28270913	28271135	28270913	ID=cds.MS.gene56077.t1;Parent=MS.gene56077.t1
chr4.4	exon	28271725	28271792	28271725	ID=MS.gene56077.t1.exon2;Parent=MS.gene56077.t1
chr4.4	CDS	28271725	28271792	28271725	ID=cds.MS.gene56077.t1;Parent=MS.gene56077.t1

Gene Sequence

>MS.gene56077

CAAGGTACAAGCAATACTATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGGTATACAGCTAGATATTATCTTTAGGGTTATTCAAGGTTCTTTTAGTGGTCAAAAGATTCCTATATTATATTTCACTCATGACATTACAAGTTACCAGCAGAGAGAGAGAGAGACAGAAAAAGATAAATAGCTTTTGTAAGGATCTCGGTAAATTTAACAAATTTACAAAATATCAATTTAGCCCATTTGGTCATTTTTTTTTCTTCAAATATAGCCCTACAATTTTAGAGATTTTAGGTTTATTCTGATGCAACAAGAAACTATAGAAGTCAATATGATATTCTTGCATAGGATAAACCTAACATCTTTAAAAGTATAGTGCCATATTTGAAAGAAAAAAATTTATTGTTGTTTATTCAATTTAATGGACTAAATTATTGCTATTTTTGAAATTTTAGGCATTAAATTAACACCCTTACAATTTGAAGTGTGTGTGAATATGTATATGTATATGAGTTGAATTTGTGGTGATTGGATTTTGAAAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAG

Protein sequence

>MS.gene56077.t1

QGTSNTMHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRQTGGGVKETKKWATTSNCSEQAE