Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56131.t1 | AES77954.1 | 90 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 2.10E-117 | 431.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56131.t1 | Q39261 | 36.9 | 84 | 45 | 3 | 87 | 170 | 52 | 127 | 4.8e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56131.t1 | G7KUQ7 | 90.0 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 1.5e-117 | 431.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56131.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56131.t1 | MTR_7g022250 | 89.958 | 239 | 24 | 0 | 1 | 239 | 1 | 239 | 2.02e-162 | 449 |
| MS.gene56131.t1 | MTR_7g028890 | 69.038 | 239 | 55 | 3 | 1 | 239 | 1 | 220 | 1.17e-116 | 332 |
| MS.gene56131.t1 | MTR_7g028915 | 68.201 | 239 | 49 | 3 | 1 | 239 | 1 | 212 | 9.40e-111 | 317 |
| MS.gene56131.t1 | MTR_7g022270 | 67.005 | 197 | 28 | 3 | 24 | 219 | 1 | 161 | 1.92e-87 | 256 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATGACTGAGATTGAATT+TGG | 0.189345 | 7.4:+79663643 | MS.gene56131:CDS |
| TTGAATTCTTAGTTTCAAAA+TGG | 0.214469 | 7.4:-79663171 | None:intergenic |
| ATTCTTCATCATCTTCAAAT+TGG | 0.223485 | 7.4:-79663104 | None:intergenic |
| CTCCTTCCCCTTGTTCTCAT+TGG | 0.229008 | 7.4:-79663059 | None:intergenic |
| CAAATTTGTGAGAACTTTGA+TGG | 0.256763 | 7.4:-79663360 | None:intergenic |
| AAGAAATGGAGGAATTTCTT+TGG | 0.278435 | 7.4:-79663239 | None:intergenic |
| ACATATAGATGAAGTGAATA+AGG | 0.341762 | 7.4:+79663591 | MS.gene56131:CDS |
| CACTGCCACTCCTTTCCATA+TGG | 0.342482 | 7.4:+79663468 | MS.gene56131:CDS |
| AGAAATGGAGGAATTTCTTT+GGG | 0.353550 | 7.4:-79663238 | None:intergenic |
| CTTAAGGGTCAAATCAATCT+TGG | 0.361873 | 7.4:-79663740 | None:intergenic |
| AATATATAGATCAAAGCTTA+AGG | 0.366337 | 7.4:-79663756 | None:intergenic |
| TCCAAGCTTCGTTAAAAGCT+TGG | 0.373123 | 7.4:-79663408 | None:intergenic |
| AATTACTCAAATGGTAATCA+AGG | 0.414383 | 7.4:+79663433 | MS.gene56131:CDS |
| TGAATTCTTAGTTTCAAAAT+GGG | 0.419158 | 7.4:-79663170 | None:intergenic |
| ACTCCTTTCCATATGGGTTG+TGG | 0.424172 | 7.4:+79663475 | MS.gene56131:CDS |
| TTCACTACTCCACGTGCATT+AGG | 0.424339 | 7.4:+79663334 | MS.gene56131:CDS |
| AACAATATGATTCAGCATGC+AGG | 0.425567 | 7.4:+79663517 | MS.gene56131:CDS |
| ATTGATATCCACAACCCATA+TGG | 0.434960 | 7.4:-79663483 | None:intergenic |
| GCTTGGATAAATTACTCAAA+TGG | 0.446981 | 7.4:+79663424 | MS.gene56131:CDS |
| TGTTAGTGACAAAGTTGATC+AGG | 0.454456 | 7.4:+79663687 | MS.gene56131:CDS |
| TCACAAATTTGAGAGAAGTC+TGG | 0.482572 | 7.4:+79663372 | MS.gene56131:CDS |
| ACTGCCACTCCTTTCCATAT+GGG | 0.486001 | 7.4:+79663469 | MS.gene56131:CDS |
| TTATTCACTTCATCTATATG+TGG | 0.494069 | 7.4:-79663589 | None:intergenic |
| TTAAGGGTCAAATCAATCTT+GGG | 0.500241 | 7.4:-79663739 | None:intergenic |
| ATATATAGATCAAAGCTTAA+GGG | 0.520066 | 7.4:-79663755 | None:intergenic |
| TGAATTTGGTTCAATTTCAG+AGG | 0.531449 | 7.4:+79663657 | MS.gene56131:CDS |
| TTGGATTCATTGTTGATCAG+AGG | 0.552579 | 7.4:+79663266 | MS.gene56131:CDS |
| GAAGTGAATAAGGCTGATCA+AGG | 0.555258 | 7.4:+79663601 | MS.gene56131:CDS |
| TTCATTGTTGATCAGAGGAA+AGG | 0.555662 | 7.4:+79663271 | MS.gene56131:CDS |
| ACAATATGATTCAGCATGCA+GGG | 0.561522 | 7.4:+79663518 | MS.gene56131:CDS |
| GTGCAACACCAATGAGAACA+AGG | 0.569603 | 7.4:+79663051 | MS.gene56131:CDS |
| CAAGCGAAAGGCCATCGTGA+TGG | 0.579476 | 7.4:+79663141 | MS.gene56131:CDS |
| GAATTTGGTTCAATTTCAGA+GGG | 0.586650 | 7.4:+79663658 | MS.gene56131:CDS |
| ATTACTCAAATGGTAATCAA+GGG | 0.589859 | 7.4:+79663434 | MS.gene56131:CDS |
| TGAGAACAAGGGGAAGGAGA+AGG | 0.594646 | 7.4:+79663063 | MS.gene56131:CDS |
| TTTGGTTCAATTTCAGAGGG+AGG | 0.596815 | 7.4:+79663661 | MS.gene56131:CDS |
| CACCAATGAGAACAAGGGGA+AGG | 0.620535 | 7.4:+79663057 | MS.gene56131:CDS |
| TATCCACAACCCATATGGAA+AGG | 0.633718 | 7.4:-79663478 | None:intergenic |
| GGAAGACAATGAGACTCAAG+AGG | 0.639538 | 7.4:+79663708 | MS.gene56131:CDS |
| ACTACTCCACGTGCATTAGG+TGG | 0.640042 | 7.4:+79663337 | MS.gene56131:CDS |
| TCATCTATATGTGGAATATG+CGG | 0.642794 | 7.4:-79663580 | None:intergenic |
| GCAACACCAATGAGAACAAG+GGG | 0.642885 | 7.4:+79663053 | MS.gene56131:CDS |
| TGCAACACCAATGAGAACAA+GGG | 0.650590 | 7.4:+79663052 | MS.gene56131:CDS |
| GAACAATGTGAACAAGCGAA+AGG | 0.655169 | 7.4:+79663129 | MS.gene56131:CDS |
| ACAACCCATATGGAAAGGAG+TGG | 0.680303 | 7.4:-79663473 | None:intergenic |
| AGACAATGAGACTCAAGAGG+AGG | 0.680897 | 7.4:+79663711 | MS.gene56131:CDS |
| ATGGGATTCTTCCATCACGA+TGG | 0.687731 | 7.4:-79663152 | None:intergenic |
| TGATGGCCACCTAATGCACG+TGG | 0.714086 | 7.4:-79663343 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTCTTAGTTTCAAAAT+GGG | - | chr7.4:79663173-79663192 | None:intergenic | 20.0% |
| !! | TTGAATTCTTAGTTTCAAAA+TGG | - | chr7.4:79663174-79663193 | None:intergenic | 20.0% |
| !!! | TTCTTCAACAATATCTTTTT+GGG | - | chr7.4:79663203-79663222 | None:intergenic | 20.0% |
| ! | AATATGACTGAGATTGAATT+TGG | + | chr7.4:79663643-79663662 | MS.gene56131:CDS | 25.0% |
| ! | AATTACTCAAATGGTAATCA+AGG | + | chr7.4:79663433-79663452 | MS.gene56131:CDS | 25.0% |
| ! | ACATATAGATGAAGTGAATA+AGG | + | chr7.4:79663591-79663610 | MS.gene56131:CDS | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.4:79663256-79663275 | None:intergenic | 25.0% |
| ! | ATTACTCAAATGGTAATCAA+GGG | + | chr7.4:79663434-79663453 | MS.gene56131:CDS | 25.0% |
| ! | ATTCTTCATCATCTTCAAAT+TGG | - | chr7.4:79663107-79663126 | None:intergenic | 25.0% |
| ! | TTATTCACTTCATCTATATG+TGG | - | chr7.4:79663592-79663611 | None:intergenic | 25.0% |
| !! | CTTCTTCAACAATATCTTTT+TGG | - | chr7.4:79663204-79663223 | None:intergenic | 25.0% |
| !! | TCTTCAACAATATCTTTTTG+GGG | - | chr7.4:79663202-79663221 | None:intergenic | 25.0% |
| !!! | TTTTATGCAGGAAATTTGTT+TGG | + | chr7.4:79663550-79663569 | MS.gene56131:CDS | 25.0% |
| AAGAAATGGAGGAATTTCTT+TGG | - | chr7.4:79663242-79663261 | None:intergenic | 30.0% | |
| AATCCAAAAAGAAGAAATGG+AGG | - | chr7.4:79663253-79663272 | None:intergenic | 30.0% | |
| AGAAATGGAGGAATTTCTTT+GGG | - | chr7.4:79663241-79663260 | None:intergenic | 30.0% | |
| CAAATTTGTGAGAACTTTGA+TGG | - | chr7.4:79663363-79663382 | None:intergenic | 30.0% | |
| GCTTGGATAAATTACTCAAA+TGG | + | chr7.4:79663424-79663443 | MS.gene56131:CDS | 30.0% | |
| TCATCTATATGTGGAATATG+CGG | - | chr7.4:79663583-79663602 | None:intergenic | 30.0% | |
| TTAAGGGTCAAATCAATCTT+GGG | - | chr7.4:79663742-79663761 | None:intergenic | 30.0% | |
| ! | ATTCCTCCATTTCTTCTTTT+TGG | + | chr7.4:79663247-79663266 | MS.gene56131:CDS | 30.0% |
| ! | GAATTTGGTTCAATTTCAGA+GGG | + | chr7.4:79663658-79663677 | MS.gene56131:CDS | 30.0% |
| ! | TGAATTTGGTTCAATTTCAG+AGG | + | chr7.4:79663657-79663676 | MS.gene56131:CDS | 30.0% |
| AACAATATGATTCAGCATGC+AGG | + | chr7.4:79663517-79663536 | MS.gene56131:CDS | 35.0% | |
| ACAATATGATTCAGCATGCA+GGG | + | chr7.4:79663518-79663537 | MS.gene56131:CDS | 35.0% | |
| AGAGAAGTCTGGTCAAAAAA+AGG | + | chr7.4:79663383-79663402 | MS.gene56131:CDS | 35.0% | |
| ATTGATATCCACAACCCATA+TGG | - | chr7.4:79663486-79663505 | None:intergenic | 35.0% | |
| CTTAAGGGTCAAATCAATCT+TGG | - | chr7.4:79663743-79663762 | None:intergenic | 35.0% | |
| TCACAAATTTGAGAGAAGTC+TGG | + | chr7.4:79663372-79663391 | MS.gene56131:CDS | 35.0% | |
| TTCATTGTTGATCAGAGGAA+AGG | + | chr7.4:79663271-79663290 | MS.gene56131:CDS | 35.0% | |
| TTGGATTCATTGTTGATCAG+AGG | + | chr7.4:79663266-79663285 | MS.gene56131:CDS | 35.0% | |
| ! | TGTTAGTGACAAAGTTGATC+AGG | + | chr7.4:79663687-79663706 | MS.gene56131:CDS | 35.0% |
| GAACAATGTGAACAAGCGAA+AGG | + | chr7.4:79663129-79663148 | MS.gene56131:CDS | 40.0% | |
| GAAGTGAATAAGGCTGATCA+AGG | + | chr7.4:79663601-79663620 | MS.gene56131:CDS | 40.0% | |
| TATCCACAACCCATATGGAA+AGG | - | chr7.4:79663481-79663500 | None:intergenic | 40.0% | |
| TCCAAGCTTCGTTAAAAGCT+TGG | - | chr7.4:79663411-79663430 | None:intergenic | 40.0% | |
| TGCAACACCAATGAGAACAA+GGG | + | chr7.4:79663052-79663071 | MS.gene56131:CDS | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7.4:79663538-79663557 | MS.gene56131:CDS | 40.0% |
| ! | TCCAAGCTTTTAACGAAGCT+TGG | + | chr7.4:79663407-79663426 | MS.gene56131:CDS | 40.0% |
| ! | TTTGGTTCAATTTCAGAGGG+AGG | + | chr7.4:79663661-79663680 | MS.gene56131:CDS | 40.0% |
| ACAACCCATATGGAAAGGAG+TGG | - | chr7.4:79663476-79663495 | None:intergenic | 45.0% | |
| ACTGCCACTCCTTTCCATAT+GGG | + | chr7.4:79663469-79663488 | MS.gene56131:CDS | 45.0% | |
| AGACAATGAGACTCAAGAGG+AGG | + | chr7.4:79663711-79663730 | MS.gene56131:CDS | 45.0% | |
| ATGGGATTCTTCCATCACGA+TGG | - | chr7.4:79663155-79663174 | None:intergenic | 45.0% | |
| GCAACACCAATGAGAACAAG+GGG | + | chr7.4:79663053-79663072 | MS.gene56131:CDS | 45.0% | |
| GGAAGACAATGAGACTCAAG+AGG | + | chr7.4:79663708-79663727 | MS.gene56131:CDS | 45.0% | |
| GTGCAACACCAATGAGAACA+AGG | + | chr7.4:79663051-79663070 | MS.gene56131:CDS | 45.0% | |
| TTCACTACTCCACGTGCATT+AGG | + | chr7.4:79663334-79663353 | MS.gene56131:CDS | 45.0% | |
| ! | ACTCCTTTCCATATGGGTTG+TGG | + | chr7.4:79663475-79663494 | MS.gene56131:CDS | 45.0% |
| ACTACTCCACGTGCATTAGG+TGG | + | chr7.4:79663337-79663356 | MS.gene56131:CDS | 50.0% | |
| CACCAATGAGAACAAGGGGA+AGG | + | chr7.4:79663057-79663076 | MS.gene56131:CDS | 50.0% | |
| CACTGCCACTCCTTTCCATA+TGG | + | chr7.4:79663468-79663487 | MS.gene56131:CDS | 50.0% | |
| CTCCTTCCCCTTGTTCTCAT+TGG | - | chr7.4:79663062-79663081 | None:intergenic | 50.0% | |
| TGAGAACAAGGGGAAGGAGA+AGG | + | chr7.4:79663063-79663082 | MS.gene56131:CDS | 50.0% | |
| CAAGCGAAAGGCCATCGTGA+TGG | + | chr7.4:79663141-79663160 | MS.gene56131:CDS | 55.0% | |
| TGATGGCCACCTAATGCACG+TGG | - | chr7.4:79663346-79663365 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 79663049 | 79663768 | 79663049 | ID=MS.gene56131 |
| chr7.4 | mRNA | 79663049 | 79663768 | 79663049 | ID=MS.gene56131.t1;Parent=MS.gene56131 |
| chr7.4 | exon | 79663049 | 79663768 | 79663049 | ID=MS.gene56131.t1.exon1;Parent=MS.gene56131.t1 |
| chr7.4 | CDS | 79663049 | 79663768 | 79663049 | ID=cds.MS.gene56131.t1;Parent=MS.gene56131.t1 |
| Gene Sequence |
| Protein sequence |
