Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56218.t1 | XP_003598191.1 | 86.2 | 275 | 38 | 0 | 1 | 275 | 1 | 275 | 3.80E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56218.t1 | G7IVM3 | 86.2 | 275 | 38 | 0 | 1 | 275 | 1 | 275 | 2.8e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56218.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56218.t1 | MTR_3g008310 | 83.636 | 275 | 45 | 0 | 1 | 275 | 1 | 275 | 1.30e-170 | 473 |
| MS.gene56218.t1 | MTR_3g408380 | 79.359 | 281 | 40 | 3 | 1 | 275 | 1 | 269 | 6.30e-160 | 445 |
| MS.gene56218.t1 | MTR_3g408420 | 63.799 | 279 | 93 | 3 | 1 | 275 | 1 | 275 | 4.25e-113 | 327 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56218.t1 | AT3G60580 | 23.656 | 279 | 155 | 9 | 8 | 266 | 6 | 246 | 2.00e-11 | 63.5 |
Find 40 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATACTTGGTTGGTGATT+GGG | 0.258868 | 3.3:-4083911 | None:intergenic |
| TTTCTTCGATTAACAATTGA+TGG | 0.305624 | 3.3:-4083987 | None:intergenic |
| AAAATTAGTGTATAGTGATT+TGG | 0.310655 | 3.3:+4084253 | MS.gene56218:CDS |
| TGCTTTGAAATCTTTGAATC+TGG | 0.324623 | 3.3:+4084542 | MS.gene56218:CDS |
| CTTTGAATCTGGTGAGGTAT+TGG | 0.325399 | 3.3:+4084553 | MS.gene56218:CDS |
| AACAATTTAGCAAGAGAGTT+TGG | 0.341571 | 3.3:+4084464 | MS.gene56218:CDS |
| TCAGATACTTGGTTGGTGAT+TGG | 0.346299 | 3.3:-4083912 | None:intergenic |
| TGTTATGAAGTGTTTCAATC+TGG | 0.395099 | 3.3:+4084401 | MS.gene56218:CDS |
| GCAAGAGAGTTTGGTAGAAG+TGG | 0.402120 | 3.3:+4084473 | MS.gene56218:CDS |
| CAACCGAGTATTCAGATACT+TGG | 0.408432 | 3.3:-4083923 | None:intergenic |
| AGATACTTGGTTGGTGATTG+GGG | 0.424806 | 3.3:-4083910 | None:intergenic |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3.3:-4083952 | None:intergenic |
| TATTCCTACCAAAGTTTGAC+AGG | 0.460181 | 3.3:-4084058 | None:intergenic |
| CACAATCTTAAATCTTTGAA+AGG | 0.463545 | 3.3:+4084020 | MS.gene56218:CDS |
| AACACACAATTTAATGTGGC+TGG | 0.473785 | 3.3:+4084206 | MS.gene56218:CDS |
| AATTGTGCCCTGTCAAACTT+TGG | 0.474964 | 3.3:+4084050 | MS.gene56218:CDS |
| TCTTATCCAACAAACCCAAC+TGG | 0.484776 | 3.3:+4084092 | MS.gene56218:CDS |
| ATATAATGTTGTAAATGAAA+AGG | 0.485017 | 3.3:+4084502 | MS.gene56218:CDS |
| GGATTCGTAATTTGGCATAG+TGG | 0.490151 | 3.3:-4083878 | None:intergenic |
| CAAACCAAGACAATTTCATA+TGG | 0.492782 | 3.3:+4084130 | MS.gene56218:CDS |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3.3:-4083960 | None:intergenic |
| ACAATCTTAAATCTTTGAAA+GGG | 0.498765 | 3.3:+4084021 | MS.gene56218:CDS |
| TTAGCCATATGAAATTGTCT+TGG | 0.500224 | 3.3:-4084134 | None:intergenic |
| GTGTTATGCGTAAGAAAGAA+TGG | 0.501519 | 3.3:+4084300 | MS.gene56218:CDS |
| TGAATCTGGTGAGGTATTGG+AGG | 0.506890 | 3.3:+4084556 | MS.gene56218:CDS |
| CAACCAAGTATCTGAATACT+CGG | 0.520019 | 3.3:+4083920 | MS.gene56218:CDS |
| GATTCGTAATTTGGCATAGT+GGG | 0.532810 | 3.3:-4083877 | None:intergenic |
| TCCTCCCAAAGCTTTCCCAT+TGG | 0.533471 | 3.3:-4083844 | None:intergenic |
| ATCTGAATACTCGGTTGAGT+TGG | 0.542063 | 3.3:+4083929 | MS.gene56218:CDS |
| CGAGTATTCAGATACTTGGT+TGG | 0.556849 | 3.3:-4083919 | None:intergenic |
| GAAAGAATGGAAACAAATTG+AGG | 0.561440 | 3.3:+4084313 | MS.gene56218:CDS |
| GATACTTGGTTGGTGATTGG+GGG | 0.574290 | 3.3:-4083909 | None:intergenic |
| GTGCCCTGTCAAACTTTGGT+AGG | 0.575115 | 3.3:+4084054 | MS.gene56218:CDS |
| GAGTGTGTCTCATTTGTCAC+AGG | 0.578392 | 3.3:+4083814 | MS.gene56218:CDS |
| TGAAATCTTTGAATCTGGTG+AGG | 0.581368 | 3.3:+4084547 | MS.gene56218:CDS |
| GGTATTGGAGGAACACATGA+AGG | 0.590503 | 3.3:+4084568 | MS.gene56218:CDS |
| TCCAATGGGAAAGCTTTGGG+AGG | 0.618334 | 3.3:+4083843 | MS.gene56218:CDS |
| AGAGAACACACAATTTAATG+TGG | 0.642332 | 3.3:+4084202 | MS.gene56218:CDS |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3.3:+4083789 | None:intergenic |
| ATTCCTACCAAAGTTTGACA+GGG | 0.694573 | 3.3:-4084057 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAATGTTGTAAATGAAA+AGG | + | chr3.3:4084502-4084521 | MS.gene56218:CDS | 15.0% |
| !!! | CTAAATTGTTAGTTTTATTA+TGG | - | chr3.3:4084454-4084473 | None:intergenic | 15.0% |
| !! | AAAATTAGTGTATAGTGATT+TGG | + | chr3.3:4084253-4084272 | MS.gene56218:CDS | 20.0% |
| !!! | ACAATCTTAAATCTTTGAAA+GGG | + | chr3.3:4084021-4084040 | MS.gene56218:CDS | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3.3:4084353-4084372 | MS.gene56218:CDS | 25.0% |
| ! | CACAATCTTAAATCTTTGAA+AGG | + | chr3.3:4084020-4084039 | MS.gene56218:CDS | 25.0% |
| ! | TTTCTTCGATTAACAATTGA+TGG | - | chr3.3:4083990-4084009 | None:intergenic | 25.0% |
| AACAATTTAGCAAGAGAGTT+TGG | + | chr3.3:4084464-4084483 | MS.gene56218:CDS | 30.0% | |
| AGAGAACACACAATTTAATG+TGG | + | chr3.3:4084202-4084221 | MS.gene56218:CDS | 30.0% | |
| CAAACCAAGACAATTTCATA+TGG | + | chr3.3:4084130-4084149 | MS.gene56218:CDS | 30.0% | |
| GAAAGAATGGAAACAAATTG+AGG | + | chr3.3:4084313-4084332 | MS.gene56218:CDS | 30.0% | |
| TTAGCCATATGAAATTGTCT+TGG | - | chr3.3:4084137-4084156 | None:intergenic | 30.0% | |
| ! | CAATCTGGTAAAGATCTTTT+CGG | + | chr3.3:4084416-4084435 | MS.gene56218:CDS | 30.0% |
| ! | TGTTATGAAGTGTTTCAATC+TGG | + | chr3.3:4084401-4084420 | MS.gene56218:CDS | 30.0% |
| !! | GTTTTGATCTTTTTCCAGTT+GGG | - | chr3.3:4084109-4084128 | None:intergenic | 30.0% |
| !! | TGCTTTGAAATCTTTGAATC+TGG | + | chr3.3:4084542-4084561 | MS.gene56218:CDS | 30.0% |
| AACACACAATTTAATGTGGC+TGG | + | chr3.3:4084206-4084225 | MS.gene56218:CDS | 35.0% | |
| ATTCCTACCAAAGTTTGACA+GGG | - | chr3.3:4084060-4084079 | None:intergenic | 35.0% | |
| CAACCAAGTATCTGAATACT+CGG | + | chr3.3:4083920-4083939 | MS.gene56218:CDS | 35.0% | |
| GATTCGTAATTTGGCATAGT+GGG | - | chr3.3:4083880-4083899 | None:intergenic | 35.0% | |
| GTGTTATGCGTAAGAAAGAA+TGG | + | chr3.3:4084300-4084319 | MS.gene56218:CDS | 35.0% | |
| TATTCCTACCAAAGTTTGAC+AGG | - | chr3.3:4084061-4084080 | None:intergenic | 35.0% | |
| TGAAATCTTTGAATCTGGTG+AGG | + | chr3.3:4084547-4084566 | MS.gene56218:CDS | 35.0% | |
| ! | ATTTTCCAATGGGAAAGCTT+TGG | + | chr3.3:4083839-4083858 | MS.gene56218:CDS | 35.0% |
| ! | TTTTCCAATGGGAAAGCTTT+GGG | + | chr3.3:4083840-4083859 | MS.gene56218:CDS | 35.0% |
| !! | CGTTTTGATCTTTTTCCAGT+TGG | - | chr3.3:4084110-4084129 | None:intergenic | 35.0% |
| !! | TTTTGGAGGGATTCGTAATT+TGG | - | chr3.3:4083889-4083908 | None:intergenic | 35.0% |
| AATTGTGCCCTGTCAAACTT+TGG | + | chr3.3:4084050-4084069 | MS.gene56218:CDS | 40.0% | |
| ATCTGAATACTCGGTTGAGT+TGG | + | chr3.3:4083929-4083948 | MS.gene56218:CDS | 40.0% | |
| CAACCGAGTATTCAGATACT+TGG | - | chr3.3:4083926-4083945 | None:intergenic | 40.0% | |
| CGAGTATTCAGATACTTGGT+TGG | - | chr3.3:4083922-4083941 | None:intergenic | 40.0% | |
| CTTTGAATCTGGTGAGGTAT+TGG | + | chr3.3:4084553-4084572 | MS.gene56218:CDS | 40.0% | |
| GATGTAGAGATAGAGTGAGT+TGG | - | chr3.3:4083963-4083982 | None:intergenic | 40.0% | |
| GGATTCGTAATTTGGCATAG+TGG | - | chr3.3:4083881-4083900 | None:intergenic | 40.0% | |
| TCTTATCCAACAAACCCAAC+TGG | + | chr3.3:4084092-4084111 | MS.gene56218:CDS | 40.0% | |
| ! | CTTTTTCCAGTTGGGTTTGT+TGG | - | chr3.3:4084101-4084120 | None:intergenic | 40.0% |
| ! | GATAGAGTGAGTTGGATGTT+TGG | - | chr3.3:4083955-4083974 | None:intergenic | 40.0% |
| !! | AGATACTTGGTTGGTGATTG+GGG | - | chr3.3:4083913-4083932 | None:intergenic | 40.0% |
| !! | CAGATACTTGGTTGGTGATT+GGG | - | chr3.3:4083914-4083933 | None:intergenic | 40.0% |
| !! | GTCACAGGTCATTTTCCAAT+GGG | + | chr3.3:4083829-4083848 | MS.gene56218:CDS | 40.0% |
| !! | TCAGATACTTGGTTGGTGAT+TGG | - | chr3.3:4083915-4083934 | None:intergenic | 40.0% |
| !! | TGTCACAGGTCATTTTCCAA+TGG | + | chr3.3:4083828-4083847 | MS.gene56218:CDS | 40.0% |
| GAGTGTGTCTCATTTGTCAC+AGG | + | chr3.3:4083814-4083833 | MS.gene56218:CDS | 45.0% | |
| GCAAGAGAGTTTGGTAGAAG+TGG | + | chr3.3:4084473-4084492 | MS.gene56218:CDS | 45.0% | |
| ! | GGTATTGGAGGAACACATGA+AGG | + | chr3.3:4084568-4084587 | MS.gene56218:CDS | 45.0% |
| !! | GATACTTGGTTGGTGATTGG+GGG | - | chr3.3:4083912-4083931 | None:intergenic | 45.0% |
| !! | TGAATCTGGTGAGGTATTGG+AGG | + | chr3.3:4084556-4084575 | MS.gene56218:CDS | 45.0% |
| GTGCCCTGTCAAACTTTGGT+AGG | + | chr3.3:4084054-4084073 | MS.gene56218:CDS | 50.0% | |
| TCCTCCCAAAGCTTTCCCAT+TGG | - | chr3.3:4083847-4083866 | None:intergenic | 50.0% | |
| !! | TCCAATGGGAAAGCTTTGGG+AGG | + | chr3.3:4083843-4083862 | MS.gene56218:CDS | 50.0% |
| !! | TTGGTGATTGGGGGTTTTGG+AGG | - | chr3.3:4083903-4083922 | None:intergenic | 50.0% |
| !!! | TGGTGATTGGGGGTTTTGGA+GGG | - | chr3.3:4083902-4083921 | None:intergenic | 50.0% |
| !!! | TGGTTGGTGATTGGGGGTTT+TGG | - | chr3.3:4083906-4083925 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4083798 | 4084625 | 4083798 | ID=MS.gene56218 |
| chr3.3 | mRNA | 4083798 | 4084625 | 4083798 | ID=MS.gene56218.t1;Parent=MS.gene56218 |
| chr3.3 | exon | 4083798 | 4084625 | 4083798 | ID=MS.gene56218.t1.exon1;Parent=MS.gene56218.t1 |
| chr3.3 | CDS | 4083798 | 4084625 | 4083798 | ID=cds.MS.gene56218.t1;Parent=MS.gene56218.t1 |
| Gene Sequence |
| Protein sequence |
