Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56222.t1 | XP_013459206.1 | 79.6 | 269 | 49 | 1 | 1 | 263 | 1 | 269 | 6.30E-99 | 370.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56222.t1 | Q9M202 | 26.0 | 269 | 149 | 10 | 8 | 254 | 6 | 246 | 1.4e-09 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56222.t1 | A0A072V3Z9 | 79.6 | 269 | 49 | 1 | 1 | 263 | 1 | 269 | 4.5e-99 | 370.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56222.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56222.t1 | MTR_3g408380 | 91.078 | 269 | 18 | 1 | 1 | 263 | 1 | 269 | 0.0 | 505 |
| MS.gene56222.t1 | MTR_3g008310 | 84.000 | 275 | 32 | 1 | 1 | 263 | 1 | 275 | 1.02e-169 | 469 |
| MS.gene56222.t1 | MTR_3g408420 | 64.493 | 276 | 84 | 3 | 1 | 263 | 1 | 275 | 7.92e-114 | 328 |
| MS.gene56222.t1 | MTR_0160s0030 | 26.797 | 306 | 164 | 10 | 8 | 255 | 6 | 309 | 8.79e-14 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56222.t1 | AT3G60580 | 26.766 | 269 | 147 | 10 | 8 | 254 | 6 | 246 | 1.59e-13 | 69.3 |
| MS.gene56222.t1 | AT1G02030 | 26.482 | 253 | 152 | 8 | 8 | 254 | 7 | 231 | 1.58e-12 | 66.2 |
Find 43 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGTTTGGTGGGTTACTTCT+TGG | 0.249003 | 3.3:-4155823 | None:intergenic |
| CAGGTACTTGGTTGTTGATT+GGG | 0.275325 | 3.3:-4155732 | None:intergenic |
| TCAGGTACTTGGTTGTTGAT+TGG | 0.290946 | 3.3:-4155733 | None:intergenic |
| CTTCTTGGATTAACAATTGA+TGG | 0.303146 | 3.3:-4155808 | None:intergenic |
| GCCAACTCAGCTGAGTATTC+AGG | 0.304238 | 3.3:-4155751 | None:intergenic |
| TGCTTTGACATCTTTGAATC+TGG | 0.309104 | 3.3:+4156327 | MS.gene56222:CDS |
| TTCAAAGATTGAGGATTGTT+TGG | 0.332480 | 3.3:-4155838 | None:intergenic |
| CTTTGAATCTGGTCACGTAT+TGG | 0.390852 | 3.3:+4156338 | MS.gene56222:CDS |
| ACAATCAGCGTATAGCGATT+TGG | 0.398813 | 3.3:+4156038 | MS.gene56222:CDS |
| CAGCTGAGTATTCAGGTACT+TGG | 0.400249 | 3.3:-4155744 | None:intergenic |
| GAGTGTGTCTCATTTGTAAC+AGG | 0.418825 | 3.3:+4155635 | MS.gene56222:CDS |
| AGGTACTTGGTTGTTGATTG+GGG | 0.434003 | 3.3:-4155731 | None:intergenic |
| CAGAATCATAATAATTGTAT+AGG | 0.437191 | 3.3:-4156380 | None:intergenic |
| GTGTTATTCTTAAGAATGAA+TGG | 0.439493 | 3.3:+4156085 | MS.gene56222:CDS |
| AATTTCACTTTCAAAGATTG+AGG | 0.444853 | 3.3:-4155847 | None:intergenic |
| AAATGTACCCCGTCAAACTT+TGG | 0.446972 | 3.3:+4155871 | MS.gene56222:CDS |
| GATAGAGTGAGTTGGATGTT+TGG | 0.448812 | 3.3:-4155773 | None:intergenic |
| AACAATCTAGCAGGAGAGAT+TGG | 0.456742 | 3.3:+4156249 | MS.gene56222:CDS |
| GGATTGGTAGTTTGGCATAG+TGG | 0.460724 | 3.3:-4155699 | None:intergenic |
| TCTTATCCAACAAACCCAAC+TGG | 0.466226 | 3.3:+4155913 | MS.gene56222:CDS |
| TATTCCTACCAAAGTTTGAC+GGG | 0.471579 | 3.3:-4155879 | None:intergenic |
| AAGATTGAGGATTGTTTGGT+GGG | 0.486813 | 3.3:-4155834 | None:intergenic |
| TCAGCCATATGAAACTGTCT+TGG | 0.489788 | 3.3:-4155955 | None:intergenic |
| CTATTCCTACCAAAGTTTGA+CGG | 0.495952 | 3.3:-4155880 | None:intergenic |
| GATGTAGAGATAGAGTGAGT+TGG | 0.497788 | 3.3:-4155781 | None:intergenic |
| TGAATCTGGTCACGTATTGG+AGG | 0.509112 | 3.3:+4156341 | MS.gene56222:CDS |
| AAAGATTGAGGATTGTTTGG+TGG | 0.514150 | 3.3:-4155835 | None:intergenic |
| GATTGGTAGTTTGGCATAGT+GGG | 0.520357 | 3.3:-4155698 | None:intergenic |
| CAAACCAAGACAGTTTCATA+TGG | 0.521057 | 3.3:+4155951 | MS.gene56222:CDS |
| GCAGGAGAGATTGGTAGAAG+TGG | 0.524466 | 3.3:+4156258 | MS.gene56222:CDS |
| AGGAAAACTAACAATCTAGC+AGG | 0.531732 | 3.3:+4156240 | MS.gene56222:CDS |
| ACCTGAATACTCAGCTGAGT+TGG | 0.539000 | 3.3:+4155750 | MS.gene56222:CDS |
| CGTATTGGAGGAACACATGA+AGG | 0.564625 | 3.3:+4156353 | MS.gene56222:CDS |
| GGTACTTGGTTGTTGATTGG+GGG | 0.573211 | 3.3:-4155730 | None:intergenic |
| GAATGAATGGAAACAAATTG+AGG | 0.585752 | 3.3:+4156098 | MS.gene56222:CDS |
| TCCAATGGAAAAGCTTTGGG+AGG | 0.590411 | 3.3:+4155664 | MS.gene56222:CDS |
| TCGGACATGAAGATATCCAT+AGG | 0.592226 | 3.3:+4156220 | MS.gene56222:CDS |
| ATAGAAGCTGCTGAAACACT+AGG | 0.609115 | 3.3:+4156063 | MS.gene56222:CDS |
| GTACCCCGTCAAACTTTGGT+AGG | 0.631191 | 3.3:+4155875 | MS.gene56222:CDS |
| ACAAATATCACACTCAAACA+CGG | 0.640237 | 3.3:-4156166 | None:intergenic |
| TCTCCAACTATGGAGAACAA+TGG | 0.649885 | 3.3:+4155610 | None:intergenic |
| AGAGAATATAGAATTTAACA+TGG | 0.705093 | 3.3:+4155987 | MS.gene56222:CDS |
| ATTCCTACCAAAGTTTGACG+GGG | 0.741422 | 3.3:-4155878 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAGAATATAGAATTTAACA+TGG | + | chr3.3:4155987-4156006 | MS.gene56222:CDS | 20.0% |
| !! | CAGAATCATAATAATTGTAT+AGG | - | chr3.3:4156383-4156402 | None:intergenic | 20.0% |
| ! | AAGAAAAAAGCAAGTGAAAA+TGG | + | chr3.3:4156138-4156157 | MS.gene56222:CDS | 25.0% |
| ! | AATTTCACTTTCAAAGATTG+AGG | - | chr3.3:4155850-4155869 | None:intergenic | 25.0% |
| !! | GTGTTATTCTTAAGAATGAA+TGG | + | chr3.3:4156085-4156104 | MS.gene56222:CDS | 25.0% |
| ACAAATATCACACTCAAACA+CGG | - | chr3.3:4156169-4156188 | None:intergenic | 30.0% | |
| CTTCTTGGATTAACAATTGA+TGG | - | chr3.3:4155811-4155830 | None:intergenic | 30.0% | |
| GAATGAATGGAAACAAATTG+AGG | + | chr3.3:4156098-4156117 | MS.gene56222:CDS | 30.0% | |
| TTCAAAGATTGAGGATTGTT+TGG | - | chr3.3:4155841-4155860 | None:intergenic | 30.0% | |
| ! | ATTTTCCAATGGAAAAGCTT+TGG | + | chr3.3:4155660-4155679 | MS.gene56222:CDS | 30.0% |
| ! | CAATCTAGCAAAGATCTTTT+CGG | + | chr3.3:4156201-4156220 | MS.gene56222:CDS | 30.0% |
| ! | TTTTCCAATGGAAAAGCTTT+GGG | + | chr3.3:4155661-4155680 | MS.gene56222:CDS | 30.0% |
| !! | GTTTTGATCTTTTTCCAGTT+GGG | - | chr3.3:4155930-4155949 | None:intergenic | 30.0% |
| !!! | CTAGATTGTTAGTTTTCCTA+TGG | - | chr3.3:4156239-4156258 | None:intergenic | 30.0% |
| AAAGATTGAGGATTGTTTGG+TGG | - | chr3.3:4155838-4155857 | None:intergenic | 35.0% | |
| AAGATTGAGGATTGTTTGGT+GGG | - | chr3.3:4155837-4155856 | None:intergenic | 35.0% | |
| AGGAAAACTAACAATCTAGC+AGG | + | chr3.3:4156240-4156259 | MS.gene56222:CDS | 35.0% | |
| CAAACCAAGACAGTTTCATA+TGG | + | chr3.3:4155951-4155970 | MS.gene56222:CDS | 35.0% | |
| CTATTCCTACCAAAGTTTGA+CGG | - | chr3.3:4155883-4155902 | None:intergenic | 35.0% | |
| TATTCCTACCAAAGTTTGAC+GGG | - | chr3.3:4155882-4155901 | None:intergenic | 35.0% | |
| TGCTTTGACATCTTTGAATC+TGG | + | chr3.3:4156327-4156346 | MS.gene56222:CDS | 35.0% | |
| !! | CGTTTTGATCTTTTTCCAGT+TGG | - | chr3.3:4155931-4155950 | None:intergenic | 35.0% |
| !! | TGTAACAGGTCATTTTCCAA+TGG | + | chr3.3:4155649-4155668 | MS.gene56222:CDS | 35.0% |
| !!! | TTTTGGATGGATTGGTAGTT+TGG | - | chr3.3:4155710-4155729 | None:intergenic | 35.0% |
| AAATGTACCCCGTCAAACTT+TGG | + | chr3.3:4155871-4155890 | MS.gene56222:CDS | 40.0% | |
| AACAATCTAGCAGGAGAGAT+TGG | + | chr3.3:4156249-4156268 | MS.gene56222:CDS | 40.0% | |
| ACAATCAGCGTATAGCGATT+TGG | + | chr3.3:4156038-4156057 | MS.gene56222:CDS | 40.0% | |
| ATAGAAGCTGCTGAAACACT+AGG | + | chr3.3:4156063-4156082 | MS.gene56222:CDS | 40.0% | |
| ATTCCTACCAAAGTTTGACG+GGG | - | chr3.3:4155881-4155900 | None:intergenic | 40.0% | |
| CTTTGAATCTGGTCACGTAT+TGG | + | chr3.3:4156338-4156357 | MS.gene56222:CDS | 40.0% | |
| GAGTGTGTCTCATTTGTAAC+AGG | + | chr3.3:4155635-4155654 | MS.gene56222:CDS | 40.0% | |
| GATGTAGAGATAGAGTGAGT+TGG | - | chr3.3:4155784-4155803 | None:intergenic | 40.0% | |
| TCAGCCATATGAAACTGTCT+TGG | - | chr3.3:4155958-4155977 | None:intergenic | 40.0% | |
| TCAGGTACTTGGTTGTTGAT+TGG | - | chr3.3:4155736-4155755 | None:intergenic | 40.0% | |
| TCGGACATGAAGATATCCAT+AGG | + | chr3.3:4156220-4156239 | MS.gene56222:CDS | 40.0% | |
| TCTTATCCAACAAACCCAAC+TGG | + | chr3.3:4155913-4155932 | MS.gene56222:CDS | 40.0% | |
| ! | AGGTACTTGGTTGTTGATTG+GGG | - | chr3.3:4155734-4155753 | None:intergenic | 40.0% |
| ! | CAGGTACTTGGTTGTTGATT+GGG | - | chr3.3:4155735-4155754 | None:intergenic | 40.0% |
| ! | CTTTTTCCAGTTGGGTTTGT+TGG | - | chr3.3:4155922-4155941 | None:intergenic | 40.0% |
| ! | GATAGAGTGAGTTGGATGTT+TGG | - | chr3.3:4155776-4155795 | None:intergenic | 40.0% |
| ! | GATTGGTAGTTTGGCATAGT+GGG | - | chr3.3:4155701-4155720 | None:intergenic | 40.0% |
| !! | TTGTTTGGTGGGTTACTTCT+TGG | - | chr3.3:4155826-4155845 | None:intergenic | 40.0% |
| ACCTGAATACTCAGCTGAGT+TGG | + | chr3.3:4155750-4155769 | MS.gene56222:CDS | 45.0% | |
| CAGCTGAGTATTCAGGTACT+TGG | - | chr3.3:4155747-4155766 | None:intergenic | 45.0% | |
| CGTATTGGAGGAACACATGA+AGG | + | chr3.3:4156353-4156372 | MS.gene56222:CDS | 45.0% | |
| TGAATCTGGTCACGTATTGG+AGG | + | chr3.3:4156341-4156360 | MS.gene56222:CDS | 45.0% | |
| ! | GGATTGGTAGTTTGGCATAG+TGG | - | chr3.3:4155702-4155721 | None:intergenic | 45.0% |
| ! | GGTACTTGGTTGTTGATTGG+GGG | - | chr3.3:4155733-4155752 | None:intergenic | 45.0% |
| ! | TCCTCCCAAAGCTTTTCCAT+TGG | - | chr3.3:4155668-4155687 | None:intergenic | 45.0% |
| !! | TCCAATGGAAAAGCTTTGGG+AGG | + | chr3.3:4155664-4155683 | MS.gene56222:CDS | 45.0% |
| !! | TGGTTGTTGATTGGGGGTTT+TGG | - | chr3.3:4155727-4155746 | None:intergenic | 45.0% |
| !!! | ATTGGGGGTTTTGGATGGAT+TGG | - | chr3.3:4155718-4155737 | None:intergenic | 45.0% |
| !!! | TGTTGATTGGGGGTTTTGGA+TGG | - | chr3.3:4155723-4155742 | None:intergenic | 45.0% |
| GCAGGAGAGATTGGTAGAAG+TGG | + | chr3.3:4156258-4156277 | MS.gene56222:CDS | 50.0% | |
| GCCAACTCAGCTGAGTATTC+AGG | - | chr3.3:4155754-4155773 | None:intergenic | 50.0% | |
| GTACCCCGTCAAACTTTGGT+AGG | + | chr3.3:4155875-4155894 | MS.gene56222:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4155619 | 4156410 | 4155619 | ID=MS.gene56222 |
| chr3.3 | mRNA | 4155619 | 4156410 | 4155619 | ID=MS.gene56222.t1;Parent=MS.gene56222 |
| chr3.3 | exon | 4155619 | 4156410 | 4155619 | ID=MS.gene56222.t1.exon1;Parent=MS.gene56222.t1 |
| chr3.3 | CDS | 4155619 | 4156410 | 4155619 | ID=cds.MS.gene56222.t1;Parent=MS.gene56222.t1 |
| Gene Sequence |
| Protein sequence |
