Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56224.t1 | XP_013459208.1 | 85.8 | 275 | 35 | 1 | 1 | 271 | 1 | 275 | 1.40E-117 | 432.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56224.t1 | Q9M202 | 26.9 | 279 | 144 | 10 | 7 | 262 | 5 | 246 | 9.0e-09 | 62.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56224.t1 | A0A072UTD1 | 85.8 | 275 | 35 | 1 | 1 | 271 | 1 | 275 | 1.0e-117 | 432.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56224.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56224.t1 | MTR_3g408420 | 90.909 | 275 | 21 | 1 | 1 | 271 | 1 | 275 | 3.52e-177 | 489 |
| MS.gene56224.t1 | MTR_3g008310 | 63.636 | 275 | 96 | 2 | 1 | 271 | 1 | 275 | 4.59e-116 | 334 |
| MS.gene56224.t1 | MTR_3g408380 | 62.230 | 278 | 89 | 4 | 1 | 271 | 1 | 269 | 4.95e-114 | 329 |
| MS.gene56224.t1 | MTR_0160s0030 | 25.392 | 319 | 168 | 11 | 4 | 263 | 2 | 309 | 1.07e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56224.t1 | AT1G02030 | 25.830 | 271 | 149 | 8 | 5 | 264 | 4 | 233 | 5.36e-12 | 64.7 |
| MS.gene56224.t1 | AT3G60580 | 26.354 | 277 | 148 | 10 | 7 | 262 | 5 | 246 | 1.23e-11 | 63.9 |
Find 26 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGGTCGTAGACAATTTAATT+TGG | 0.272723 | 3.3:+4182141 | MS.gene56224:CDS |
| AACAATCTATCTGAAGAAAT+TGG | 0.300908 | 3.3:+4182466 | MS.gene56224:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3.3:+4182240 | MS.gene56224:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3.3:+4182342 | MS.gene56224:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3.3:+4182255 | MS.gene56224:CDS |
| CTATTATTTGTGTGAATGAA+TGG | 0.399412 | 3.3:+4182302 | MS.gene56224:CDS |
| TCACGTGCTTGGTTGTTGAT+TGG | 0.399497 | 3.3:-4181926 | None:intergenic |
| TATCATACCCTTGTAAACTT+TGG | 0.406116 | 3.3:+4182061 | MS.gene56224:CDS |
| TCTGAAGAAATTGGTATAAG+TGG | 0.426032 | 3.3:+4182475 | MS.gene56224:CDS |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3.3:-4181966 | None:intergenic |
| TACAGAAGCTGCTGAAACAT+TGG | 0.473334 | 3.3:+4182279 | MS.gene56224:CDS |
| TATTCTTACCAAAGTTTACA+AGG | 0.477139 | 3.3:-4182069 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3.3:+4182528 | MS.gene56224:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3.3:-4181852 | None:intergenic |
| AAGATCGAAGATTATGCATA+GGG | 0.485747 | 3.3:-4182024 | None:intergenic |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3.3:+4182570 | MS.gene56224:CDS |
| CAACTGAGTGTTCACGTGCT+TGG | 0.533072 | 3.3:-4181937 | None:intergenic |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3.3:+4182558 | MS.gene56224:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3.3:+4182032 | MS.gene56224:CDS |
| AAAGATCGAAGATTATGCAT+AGG | 0.557994 | 3.3:-4182025 | None:intergenic |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3.3:+4182480 | MS.gene56224:CDS |
| AGAGAATACAATATTTAATG+TGG | 0.616169 | 3.3:+4182168 | MS.gene56224:CDS |
| TGTGGCTGAAGAAAATGAAG+AGG | 0.616323 | 3.3:+4182186 | MS.gene56224:CDS |
| ATTCTTACCAAAGTTTACAA+GGG | 0.625545 | 3.3:-4182068 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3.3:+4181857 | MS.gene56224:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3.3:+4182315 | MS.gene56224:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGATTGTTATTTTTCTTA+TGG | - | chr3.3:4182456-4182475 | None:intergenic | 15.0% |
| !! | AGAGAATACAATATTTAATG+TGG | + | chr3.3:4182168-4182187 | MS.gene56224:CDS | 20.0% |
| ! | AACAATCTATCTGAAGAAAT+TGG | + | chr3.3:4182466-4182485 | MS.gene56224:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3.3:4182355-4182374 | MS.gene56224:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3.3:4182342-4182361 | MS.gene56224:CDS | 25.0% |
| ! | ATTCTTACCAAAGTTTACAA+GGG | - | chr3.3:4182071-4182090 | None:intergenic | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3.3:4182302-4182321 | MS.gene56224:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3.3:4182255-4182274 | MS.gene56224:CDS | 25.0% |
| ! | TATTCTTACCAAAGTTTACA+AGG | - | chr3.3:4182072-4182091 | None:intergenic | 25.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3.3:4182240-4182259 | MS.gene56224:CDS | 25.0% |
| !! | TGGGTTAGAATCATAAAAAT+TGG | - | chr3.3:4182605-4182624 | None:intergenic | 25.0% |
| AAAGATCGAAGATTATGCAT+AGG | - | chr3.3:4182028-4182047 | None:intergenic | 30.0% | |
| AAGATCGAAGATTATGCATA+GGG | - | chr3.3:4182027-4182046 | None:intergenic | 30.0% | |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3.3:4181969-4181988 | None:intergenic | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3.3:4182480-4182499 | MS.gene56224:CDS | 30.0% | |
| TATCATACCCTTGTAAACTT+TGG | + | chr3.3:4182061-4182080 | MS.gene56224:CDS | 30.0% | |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3.3:4182475-4182494 | MS.gene56224:CDS | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3.3:4182032-4182051 | MS.gene56224:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3.3:4181843-4181862 | MS.gene56224:CDS | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3.3:4181842-4181861 | MS.gene56224:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3.3:4181916-4181935 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3.3:4181917-4181936 | None:intergenic | 30.0% |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3.3:4182002-4182021 | None:intergenic | 30.0% |
| !!! | AGATTATGCATAGGGTTTTT+TGG | - | chr3.3:4182019-4182038 | None:intergenic | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3.3:4181854-4181873 | MS.gene56224:CDS | 30.0% |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3.3:4181855-4181874 | None:intergenic | 35.0% | |
| CGGTCGTAGACAATTTAATT+TGG | + | chr3.3:4182141-4182160 | MS.gene56224:CDS | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3.3:4182315-4182334 | MS.gene56224:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3.3:4182558-4182577 | MS.gene56224:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3.3:4182570-4182589 | MS.gene56224:CDS | 35.0% | |
| ! | CTTTTTCCAGTTGGGTTTTT+TGG | - | chr3.3:4182112-4182131 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3.3:4182120-4182139 | None:intergenic | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3.3:4181883-4181902 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAAAACCCAAC+TGG | + | chr3.3:4182103-4182122 | MS.gene56224:CDS | 35.0% |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3.3:4181853-4181872 | MS.gene56224:CDS | 35.0% |
| !!! | ATGCATAGGGTTTTTTGGAT+TGG | - | chr3.3:4182014-4182033 | None:intergenic | 35.0% |
| ACTGGAAAAAGATCGAAACG+CGG | + | chr3.3:4182121-4182140 | MS.gene56224:CDS | 40.0% | |
| TACAGAAGCTGCTGAAACAT+TGG | + | chr3.3:4182279-4182298 | MS.gene56224:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3.3:4182528-4182547 | MS.gene56224:CDS | 40.0% | |
| TGTGGCTGAAGAAAATGAAG+AGG | + | chr3.3:4182186-4182205 | MS.gene56224:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3.3:4182121-4182140 | None:intergenic | 40.0% |
| TCACGTGCTTGGTTGTTGAT+TGG | - | chr3.3:4181929-4181948 | None:intergenic | 45.0% | |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3.3:4181857-4181876 | MS.gene56224:CDS | 45.0% |
| CAACTGAGTGTTCACGTGCT+TGG | - | chr3.3:4181940-4181959 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4181812 | 4182627 | 4181812 | ID=MS.gene56224 |
| chr3.3 | mRNA | 4181812 | 4182627 | 4181812 | ID=MS.gene56224.t1;Parent=MS.gene56224 |
| chr3.3 | exon | 4181812 | 4182627 | 4181812 | ID=MS.gene56224.t1.exon1;Parent=MS.gene56224.t1 |
| chr3.3 | CDS | 4181812 | 4182627 | 4181812 | ID=cds.MS.gene56224.t1;Parent=MS.gene56224.t1 |
| Gene Sequence |
| Protein sequence |
