Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56226.t1 | XP_013459208.1 | 85.8 | 275 | 35 | 1 | 1 | 271 | 1 | 275 | 1.30E-118 | 436 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56226.t1 | Q9M202 | 24.7 | 279 | 150 | 10 | 7 | 262 | 5 | 246 | 3.4e-08 | 60.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56226.t1 | A0A072UTD1 | 85.8 | 275 | 35 | 1 | 1 | 271 | 1 | 275 | 9.0e-119 | 436.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56226.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56226.t1 | MTR_3g408420 | 90.909 | 275 | 21 | 1 | 1 | 271 | 1 | 275 | 1.10e-178 | 493 |
| MS.gene56226.t1 | MTR_3g008310 | 62.909 | 275 | 98 | 2 | 1 | 271 | 1 | 275 | 1.23e-114 | 330 |
| MS.gene56226.t1 | MTR_3g408380 | 61.511 | 278 | 91 | 4 | 1 | 271 | 1 | 269 | 2.74e-112 | 324 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56226.t1 | AT1G02030 | 25.830 | 271 | 149 | 8 | 5 | 264 | 4 | 233 | 1.71e-12 | 66.2 |
Find 27 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTCGTAGACAATTTAATT+TGG | 0.296849 | 3.3:+4201106 | MS.gene56226:CDS |
| AACAATCTATCTGAAGAAAT+TGG | 0.300908 | 3.3:+4201431 | MS.gene56226:CDS |
| TGAGAATACACAAGTTAAAT+TGG | 0.302912 | 3.3:+4201205 | MS.gene56226:CDS |
| ATACTATAGTGAGAAGAAAA+AGG | 0.312924 | 3.3:+4201307 | MS.gene56226:CDS |
| TAAATTGGTGTATAGTGATT+TGG | 0.323391 | 3.3:+4201220 | MS.gene56226:CDS |
| TCAGGTGCTTGGTTGTTGAT+TGG | 0.373001 | 3.3:-4200891 | None:intergenic |
| TATCATACCCTTGTAAACTT+TGG | 0.403944 | 3.3:+4201026 | MS.gene56226:CDS |
| CAACTGAGTGTTCAGGTGCT+TGG | 0.424891 | 3.3:-4200902 | None:intergenic |
| TCTGAAGAAATTGGTATAAG+TGG | 0.426032 | 3.3:+4201440 | MS.gene56226:CDS |
| CTATTATTTGTGTGAATGAA+TGG | 0.435873 | 3.3:+4201267 | MS.gene56226:CDS |
| AAGATCGAAGATTATGCATC+GGG | 0.436213 | 3.3:-4200989 | None:intergenic |
| GTTGACTCAACTGAGTGTTC+AGG | 0.442669 | 3.3:-4200909 | None:intergenic |
| AATAGAGAGAGTTTGATGTT+TGG | 0.448411 | 3.3:-4200931 | None:intergenic |
| TATTCTTACCAAAGTTTACA+AGG | 0.477139 | 3.3:-4201034 | None:intergenic |
| TCACAAGTGCACATATTGCT+TGG | 0.480968 | 3.3:+4201493 | MS.gene56226:CDS |
| CAATGCTTTCCCATTAGAAA+AGG | 0.484168 | 3.3:-4200817 | None:intergenic |
| TACAGAAGCTGCTGAAACAA+TGG | 0.521106 | 3.3:+4201244 | MS.gene56226:CDS |
| TGTACTTGAGGAACACAAAA+CGG | 0.523616 | 3.3:+4201535 | MS.gene56226:CDS |
| TGAATCTGATCATGTACTTG+AGG | 0.537940 | 3.3:+4201523 | MS.gene56226:CDS |
| ATAATCTTCGATCTTTGAAG+AGG | 0.557615 | 3.3:+4200997 | MS.gene56226:CDS |
| AGAAATTGGTATAAGTGGAA+AGG | 0.574832 | 3.3:+4201445 | MS.gene56226:CDS |
| AAAGATCGAAGATTATGCAT+CGG | 0.580003 | 3.3:-4200990 | None:intergenic |
| AGAGAATACAATATTTAATG+TGG | 0.616169 | 3.3:+4201133 | MS.gene56226:CDS |
| TGTGGCTGAAGAAAATGAAG+AGG | 0.616323 | 3.3:+4201151 | MS.gene56226:CDS |
| ATTCTTACCAAAGTTTACAA+GGG | 0.625545 | 3.3:-4201033 | None:intergenic |
| TCTAATGGGAAAGCATTGGG+AGG | 0.669450 | 3.3:+4200822 | MS.gene56226:CDS |
| GAATGAATGGAAACAAGTTG+AGG | 0.698163 | 3.3:+4201280 | MS.gene56226:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAGATTGTTATTTTTCTTA+TGG | - | chr3.3:4201421-4201440 | None:intergenic | 15.0% |
| !! | AGAGAATACAATATTTAATG+TGG | + | chr3.3:4201133-4201152 | MS.gene56226:CDS | 20.0% |
| ! | AACAATCTATCTGAAGAAAT+TGG | + | chr3.3:4201431-4201450 | MS.gene56226:CDS | 25.0% |
| ! | AAGAAAAAGGTTAGTGAAAA+TGG | + | chr3.3:4201320-4201339 | MS.gene56226:CDS | 25.0% |
| ! | ATACTATAGTGAGAAGAAAA+AGG | + | chr3.3:4201307-4201326 | MS.gene56226:CDS | 25.0% |
| ! | ATTCTTACCAAAGTTTACAA+GGG | - | chr3.3:4201036-4201055 | None:intergenic | 25.0% |
| ! | CTATTATTTGTGTGAATGAA+TGG | + | chr3.3:4201267-4201286 | MS.gene56226:CDS | 25.0% |
| ! | TAAATTGGTGTATAGTGATT+TGG | + | chr3.3:4201220-4201239 | MS.gene56226:CDS | 25.0% |
| ! | TATTCTTACCAAAGTTTACA+AGG | - | chr3.3:4201037-4201056 | None:intergenic | 25.0% |
| ! | TGAGAATACACAAGTTAAAT+TGG | + | chr3.3:4201205-4201224 | MS.gene56226:CDS | 25.0% |
| AAAGATCGAAGATTATGCAT+CGG | - | chr3.3:4200993-4201012 | None:intergenic | 30.0% | |
| AATAGAGAGAGTTTGATGTT+TGG | - | chr3.3:4200934-4200953 | None:intergenic | 30.0% | |
| AGAAATTGGTATAAGTGGAA+AGG | + | chr3.3:4201445-4201464 | MS.gene56226:CDS | 30.0% | |
| CAGTCGTAGACAATTTAATT+TGG | + | chr3.3:4201106-4201125 | MS.gene56226:CDS | 30.0% | |
| TATCATACCCTTGTAAACTT+TGG | + | chr3.3:4201026-4201045 | MS.gene56226:CDS | 30.0% | |
| TCTGAAGAAATTGGTATAAG+TGG | + | chr3.3:4201440-4201459 | MS.gene56226:CDS | 30.0% | |
| ! | ATAATCTTCGATCTTTGAAG+AGG | + | chr3.3:4200997-4201016 | MS.gene56226:CDS | 30.0% |
| ! | GTAACAAGTCCTTTTCTAAT+GGG | + | chr3.3:4200808-4200827 | MS.gene56226:CDS | 30.0% |
| ! | TGGGTTAGAATCAGAAAAAT+TGG | - | chr3.3:4201570-4201589 | None:intergenic | 30.0% |
| ! | TGTAACAAGTCCTTTTCTAA+TGG | + | chr3.3:4200807-4200826 | MS.gene56226:CDS | 30.0% |
| !! | TGTTGATTGGAGATTTTAGA+GGG | - | chr3.3:4200881-4200900 | None:intergenic | 30.0% |
| !! | TTGTTGATTGGAGATTTTAG+AGG | - | chr3.3:4200882-4200901 | None:intergenic | 30.0% |
| !! | TTTTGGATTGGAAATTGATG+AGG | - | chr3.3:4200967-4200986 | None:intergenic | 30.0% |
| !!! | TTTTCTAATGGGAAAGCATT+GGG | + | chr3.3:4200819-4200838 | MS.gene56226:CDS | 30.0% |
| AAGATCGAAGATTATGCATC+GGG | - | chr3.3:4200992-4201011 | None:intergenic | 35.0% | |
| CAATGCTTTCCCATTAGAAA+AGG | - | chr3.3:4200820-4200839 | None:intergenic | 35.0% | |
| GAATGAATGGAAACAAGTTG+AGG | + | chr3.3:4201280-4201299 | MS.gene56226:CDS | 35.0% | |
| TGAATCTGATCATGTACTTG+AGG | + | chr3.3:4201523-4201542 | MS.gene56226:CDS | 35.0% | |
| TGTACTTGAGGAACACAAAA+CGG | + | chr3.3:4201535-4201554 | MS.gene56226:CDS | 35.0% | |
| ! | CTTTTTCCAGTTGGGTATTT+TGG | - | chr3.3:4201077-4201096 | None:intergenic | 35.0% |
| ! | GTTTCGATCTTTTTCCAGTT+GGG | - | chr3.3:4201085-4201104 | None:intergenic | 35.0% |
| ! | TAGCTAAGTGAGTTTTCATG+TGG | - | chr3.3:4200848-4200867 | None:intergenic | 35.0% |
| ! | TCTTTTCCAAAATACCCAAC+TGG | + | chr3.3:4201068-4201087 | MS.gene56226:CDS | 35.0% |
| !! | ATTGGAGATTTTAGAGGGAT+TGG | - | chr3.3:4200876-4200895 | None:intergenic | 35.0% |
| !! | CTTTTCTAATGGGAAAGCAT+TGG | + | chr3.3:4200818-4200837 | MS.gene56226:CDS | 35.0% |
| !!! | AGATTATGCATCGGGTTTTT+TGG | - | chr3.3:4200984-4201003 | None:intergenic | 35.0% |
| TACAGAAGCTGCTGAAACAA+TGG | + | chr3.3:4201244-4201263 | MS.gene56226:CDS | 40.0% | |
| TCACAAGTGCACATATTGCT+TGG | + | chr3.3:4201493-4201512 | MS.gene56226:CDS | 40.0% | |
| TGTGGCTGAAGAAAATGAAG+AGG | + | chr3.3:4201151-4201170 | MS.gene56226:CDS | 40.0% | |
| ! | CGTTTCGATCTTTTTCCAGT+TGG | - | chr3.3:4201086-4201105 | None:intergenic | 40.0% |
| !!! | ATGCATCGGGTTTTTTGGAT+TGG | - | chr3.3:4200979-4200998 | None:intergenic | 40.0% |
| TCAGGTGCTTGGTTGTTGAT+TGG | - | chr3.3:4200894-4200913 | None:intergenic | 45.0% | |
| ! | GTTGACTCAACTGAGTGTTC+AGG | - | chr3.3:4200912-4200931 | None:intergenic | 45.0% |
| !! | TCTAATGGGAAAGCATTGGG+AGG | + | chr3.3:4200822-4200841 | MS.gene56226:CDS | 45.0% |
| CAACTGAGTGTTCAGGTGCT+TGG | - | chr3.3:4200905-4200924 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4200777 | 4201592 | 4200777 | ID=MS.gene56226 |
| chr3.3 | mRNA | 4200777 | 4201592 | 4200777 | ID=MS.gene56226.t1;Parent=MS.gene56226 |
| chr3.3 | exon | 4200777 | 4201592 | 4200777 | ID=MS.gene56226.t1.exon1;Parent=MS.gene56226.t1 |
| chr3.3 | CDS | 4200777 | 4201592 | 4200777 | ID=cds.MS.gene56226.t1;Parent=MS.gene56226.t1 |
| Gene Sequence |
| Protein sequence |
