AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56280.t1 MTR_5g015480 88.108 185 22 0 1 185 1 185 2.13e-113 332
MS.gene56280.t1 MTR_0402s0040 68.500 200 58 2 36 231 2 200 2.77e-88 276
MS.gene56280.t1 MTR_2g070590 68.908 119 37 0 40 158 1 119 4.61e-51 167
MS.gene56280.t1 MTR_6g017200 69.421 121 33 1 36 152 34 154 1.20e-50 167
MS.gene56280.t1 MTR_7g113010 55.625 160 53 2 26 181 423 568 1.60e-46 168
MS.gene56280.t1 MTR_1g057920 63.415 123 41 1 27 145 1 123 7.57e-45 151
MS.gene56280.t1 MTR_1g086270 80.282 71 14 0 250 320 1 71 3.03e-37 131
MS.gene56280.t1 MTR_0137s0040 67.045 88 25 1 25 108 1 88 4.24e-34 123
MS.gene56280.t1 MTR_7g074790 36.967 211 108 6 56 262 10 199 4.26e-27 108
MS.gene56280.t1 MTR_1g050985 78.333 60 13 0 126 185 39 98 8.30e-27 103
MS.gene56280.t1 MTR_4g102720 34.524 168 74 3 56 223 7 138 1.05e-22 95.9
MS.gene56280.t1 MTR_0291s0060 65.217 69 20 1 38 102 13 81 3.33e-22 90.5
MS.gene56280.t1 MTR_1g070355 30.806 211 131 6 23 223 37 242 9.07e-22 97.8
MS.gene56280.t1 MTR_4g067290 45.794 107 52 3 56 160 56 158 1.41e-20 90.5
MS.gene56280.t1 MTR_4g067290 45.794 107 52 3 56 160 56 158 1.60e-20 90.1
MS.gene56280.t1 MTR_4g067290 45.794 107 52 3 56 160 74 176 2.05e-20 90.1
MS.gene56280.t1 MTR_4g067290 45.794 107 52 3 56 160 76 178 2.12e-20 90.5
MS.gene56280.t1 MTR_4g067290 45.794 107 52 3 56 160 76 178 2.39e-20 90.1
MS.gene56280.t1 MTR_4g067290 45.370 108 53 3 56 161 116 219 4.17e-20 90.1
MS.gene56280.t1 MTR_2g098920 40.517 116 59 4 56 165 80 191 9.37e-19 85.9
MS.gene56280.t1 MTR_5g076070 30.380 158 107 2 62 217 59 215 1.99e-18 85.1
MS.gene56280.t1 MTR_1g091013 66.071 56 19 0 69 124 14 69 1.31e-17 77.0
MS.gene56280.t1 MTR_6g022080 30.380 158 107 2 62 217 91 247 2.21e-17 81.6
MS.gene56280.t1 MTR_7g061980 41.593 113 62 3 56 167 10 119 1.81e-16 75.5
MS.gene56280.t1 MTR_6g051425 27.429 175 118 4 63 231 24 195 3.89e-16 79.3
MS.gene56280.t1 MTR_2g099430 41.379 87 49 1 81 167 28 112 2.88e-14 69.3
MS.gene56280.t1 MTR_5g039760 34.091 176 87 6 60 233 14 162 3.31e-14 71.2
MS.gene56280.t1 MTR_3g006370 39.048 105 62 2 56 159 50 153 3.71e-14 72.0
MS.gene56280.t1 MTR_1g019850 30.488 164 104 3 34 194 45 201 2.46e-13 69.7
MS.gene56280.t1 MTR_0558s0020 25.137 183 119 4 70 234 50 232 4.57e-13 69.7
MS.gene56280.t1 MTR_7g446520 28.736 174 107 3 59 232 91 247 7.07e-13 69.7
MS.gene56280.t1 MTR_2g098880 32.414 145 92 4 42 182 47 189 7.22e-13 68.6
MS.gene56280.t1 MTR_2g098880 32.414 145 92 4 42 182 59 201 8.08e-13 68.6
MS.gene56280.t1 MTR_2g014100 23.560 191 128 4 62 234 50 240 1.05e-12 68.2
MS.gene56280.t1 MTR_1g083640 25.131 191 125 4 62 234 57 247 3.03e-12 67.0
MS.gene56280.t1 MTR_1g008290 32.000 125 80 2 108 232 3 122 5.85e-12 63.5
MS.gene56280.t1 MTR_4g035820 30.726 179 113 6 48 218 66 241 5.31e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56280.t1 AT4G38180 38.462 182 107 3 56 234 73 252 4.51e-30 123
MS.gene56280.t1 AT3G59470 46.226 106 55 2 56 161 70 173 9.17e-22 94.0
MS.gene56280.t1 AT3G59470 46.226 106 55 2 56 161 70 173 9.17e-22 94.0
MS.gene56280.t1 AT3G59470 46.226 106 55 2 56 161 82 185 1.05e-21 94.4
MS.gene56280.t1 AT1G10240 30.000 180 117 5 49 221 41 218 7.43e-17 83.2
MS.gene56280.t1 AT1G10240 30.000 180 117 5 49 221 41 218 7.43e-17 83.2
MS.gene56280.t1 AT3G07500 41.905 105 60 1 56 160 33 136 1.37e-15 75.9
MS.gene56280.t1 AT2G43280 37.121 132 74 4 52 181 18 142 2.12e-14 72.0
MS.gene56280.t1 AT4G12850 33.333 126 77 3 56 181 11 129 4.05e-13 66.6
MS.gene56280.t1 AT4G15090 28.571 154 108 2 56 209 51 202 1.23e-12 70.1
MS.gene56280.t1 AT4G15090 28.571 154 108 2 56 209 51 202 1.23e-12 70.1
MS.gene56280.t1 AT4G15090 28.571 154 108 2 56 209 51 202 1.23e-12 70.1
MS.gene56280.t1 AT4G15090 28.571 154 108 2 56 209 51 202 1.23e-12 70.1
MS.gene56280.t1 AT4G15090 28.571 154 108 2 56 209 51 202 1.23e-12 70.1
MS.gene56280.t1 AT4G12850 35.922 103 62 2 56 158 11 109 4.42e-12 63.9
MS.gene56280.t1 AT1G76320 34.586 133 76 4 60 192 1 122 4.58e-12 68.2
MS.gene56280.t1 AT1G76320 34.586 133 76 4 60 192 1 122 4.80e-12 68.2
MS.gene56280.t1 AT1G76320 34.586 133 76 4 60 192 1 122 4.80e-12 68.2
MS.gene56280.t1 AT1G76320 34.586 133 76 4 60 192 1 122 7.73e-12 67.4
MS.gene56280.t1 AT2G43280 40.659 91 50 3 71 159 2 90 7.75e-12 63.9
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 42 146 1.74e-11 66.6
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 42 146 1.74e-11 66.6
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 42 146 1.82e-11 66.6
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 42 146 1.82e-11 66.6
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 42 146 1.82e-11 66.6
MS.gene56280.t1 AT4G12850 35.922 103 62 2 56 158 11 109 1.88e-11 63.2
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 1 105 1.90e-11 66.2
MS.gene56280.t1 AT5G18960 33.051 118 65 2 41 158 28 131 1.92e-11 66.2
MS.gene56280.t1 AT5G18960 33.051 118 65 2 41 158 28 131 1.92e-11 66.2
MS.gene56280.t1 AT2G32250 35.455 110 65 3 60 168 42 146 1.94e-11 66.2
MS.gene56280.t1 AT2G32250 33.945 109 68 2 60 168 1 105 1.96e-11 66.2
MS.gene56280.t1 AT3G22170 35.593 118 62 2 60 164 74 190 8.23e-11 64.3
MS.gene56280.t1 AT3G22170 35.593 118 62 2 60 164 74 190 8.23e-11 64.3

Find 88 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCATCAGCCAATACAATT+TGG 0.204201 3.1:-5070840 None:intergenic
TTGAACTTCATCATTGCTTA+TGG 0.217146 3.1:+5071573 MS.gene56280:CDS
CACAATCCATGAAATGTTTC+TGG 0.237682 3.1:-5071381 None:intergenic
GAAATTTGCTTCGCACTAAA+AGG 0.249768 3.1:+5070950 MS.gene56280:CDS
GATTGTGCACTTGGCATATA+AGG 0.257928 3.1:+5071397 MS.gene56280:CDS
TGAACGAGAAAGGGCAGATT+TGG 0.279196 3.1:+5071858 MS.gene56280:CDS
ACAAATTCATGAAAATCTTT+TGG 0.298226 3.1:-5070874 None:intergenic
GACACCGCGTCTACCCTCTT+TGG 0.319795 3.1:-5070617 None:intergenic
ATTGTATTGGCTGATGAATC+TGG 0.322531 3.1:+5070844 MS.gene56280:CDS
CATTTCATGGATTGTGCACT+TGG 0.334162 3.1:+5071388 MS.gene56280:CDS
TAGTGCGAAGCAAATTTCTA+TGG 0.349224 3.1:-5070945 None:intergenic
TATAGTAGGAAATGTGGTTT+TGG 0.369598 3.1:+5070526 MS.gene56280:CDS
CGCGACGGTGGCAGTGGCGG+AGG 0.371954 3.1:+5071936 MS.gene56280:CDS
TTCATCAGCCAATACAATTT+GGG 0.375471 3.1:-5070839 None:intergenic
GGTGAATGATATCGTGCAAC+AGG 0.381657 3.1:+5070420 MS.gene56280:CDS
TCACCATTCATCACCACCTC+TGG 0.385515 3.1:+5070205 MS.gene56280:CDS
TTCATCATTGCTTATGGACT+TGG 0.386241 3.1:+5071579 MS.gene56280:CDS
ATTTCACTTGATCGGAAGAT+TGG 0.399360 3.1:+5070709 MS.gene56280:CDS
GTCATGCCAGAAACATTTCA+TGG 0.400517 3.1:+5071375 MS.gene56280:CDS
ATAGATGATTCACATGCCTT+AGG 0.411459 3.1:-5071428 None:intergenic
TTAGAAAACATGAACGAGAA+AGG 0.427796 3.1:+5071848 MS.gene56280:CDS
TAATGTACCACACTTGGAAA+TGG 0.430430 3.1:+5070462 MS.gene56280:CDS
TACACCTTCAACCATTGGAT+TGG 0.436578 3.1:-5070389 None:intergenic
ACGTGTGTCGTTTCTACCAT+TGG 0.448381 3.1:-5070164 None:intergenic
TAGATGATTCACATGCCTTA+GGG 0.455627 3.1:-5071427 None:intergenic
GAACGAGAAAGGGCAGATTT+GGG 0.457260 3.1:+5071859 MS.gene56280:CDS
TATGGTGCTGGCTTCTAGAA+AGG 0.460860 3.1:+5071896 MS.gene56280:CDS
AACAGGATGTTTAGCACGTA+TGG 0.468473 3.1:+5070684 MS.gene56280:CDS
TGCATCTTGATCTGTTAGAA+TGG 0.469730 3.1:-5071328 None:intergenic
GGAGTTTGAGTCTGAGGCCT+TGG 0.475074 3.1:+5070483 MS.gene56280:CDS
TAGAAAACATGAACGAGAAA+GGG 0.477194 3.1:+5071849 MS.gene56280:CDS
AAGAGGCGCGACGGTGGCAG+TGG 0.486772 3.1:+5071930 MS.gene56280:CDS
AGGCGCGACGGTGGCAGTGG+CGG 0.494164 3.1:+5071933 MS.gene56280:CDS
ATAAGCAATGATGAAGTTCA+AGG 0.495805 3.1:-5071571 None:intergenic
ACAAGCATCCCAAATTGTAT+TGG 0.495869 3.1:+5070831 MS.gene56280:CDS
TCACTAAGGTATGGTGAAGT+AGG 0.507546 3.1:+5070979 MS.gene56280:CDS
GCGACGGTGGCAGTGGCGGA+GGG 0.509515 3.1:+5071937 MS.gene56280:CDS
ATTGTGCACTTGGCATATAA+GGG 0.522000 3.1:+5071398 MS.gene56280:CDS
ATCATATAAACATACTCAGC+TGG 0.522562 3.1:-5070781 None:intergenic
CATACTCAGCTGGCAGAAGA+AGG 0.525063 3.1:-5070771 None:intergenic
GAGGGCTTTGCTTTGAGATG+CGG 0.525523 3.1:+5071955 MS.gene56280:CDS
ATTTAGTAATGTACCACACT+TGG 0.526810 3.1:+5070456 MS.gene56280:CDS
AAAAGACAACAAAGACATGA+AGG 0.528719 3.1:-5071827 None:intergenic
CACCATTCATCACCACCTCT+GGG 0.529997 3.1:+5070206 MS.gene56280:CDS
ACATCCAATCCAATGGTTGA+AGG 0.531051 3.1:+5070385 MS.gene56280:CDS
GTATGGTTATTTCACTTGAT+CGG 0.532614 3.1:+5070701 MS.gene56280:CDS
GCTCGAGCAGAAACTAGAAC+AGG 0.536024 3.1:+5070667 MS.gene56280:CDS
TTTGCTTTGAGATGCGGCGA+CGG 0.537102 3.1:+5071961 MS.gene56280:CDS
ATGGTGCTGGCTTCTAGAAA+GGG 0.539263 3.1:+5071897 MS.gene56280:CDS
TGCGGCGACGGCAGAAGTGA+CGG 0.540025 3.1:+5071973 MS.gene56280:CDS
TCAAGATGCAGCAATGGCTA+AGG 0.541612 3.1:+5071341 MS.gene56280:CDS
ACGTGATTACTTAACAAAAG+AGG 0.543276 3.1:+5070645 MS.gene56280:CDS
CTAAAAGGAACCACTCACTA+AGG 0.543419 3.1:+5070965 MS.gene56280:CDS
TGCCCAGAGGTGGTGATGAA+TGG 0.545146 3.1:-5070208 None:intergenic
GGAAATGGAGTTTGAGTCTG+AGG 0.549669 3.1:+5070477 MS.gene56280:CDS
AATGAGTATAGTAGGAAATG+TGG 0.549866 3.1:+5070520 MS.gene56280:CDS
ACCTTTCTCAAAGCAATGGG+TGG 0.552305 3.1:+5071291 MS.gene56280:intron
CAAGGTTATTGACTTTGTAA+CGG 0.554403 3.1:+5070738 MS.gene56280:CDS
TCGGAAGATTGGAAAATACA+AGG 0.556421 3.1:+5070720 MS.gene56280:CDS
ATTGAAGAATTAGAATGCCA+AGG 0.561345 3.1:+5071799 MS.gene56280:CDS
ATCTTACCGGTGCCCAGAGG+TGG 0.565589 3.1:-5070218 None:intergenic
ATTATAAAATTCATATGCCA+AGG 0.568982 3.1:-5070500 None:intergenic
TGGGTCGTCACAGTAACATA+TGG 0.569464 3.1:+5071878 MS.gene56280:CDS
AACAGATCAAGATGCAGCAA+TGG 0.573110 3.1:+5071335 MS.gene56280:CDS
AGGAACCACTCACTAAGGTA+TGG 0.576328 3.1:+5070970 MS.gene56280:CDS
GTCACAGTAACATATGGTGC+TGG 0.579456 3.1:+5071884 MS.gene56280:CDS
TTGAAGAATTAGAATGCCAA+GGG 0.582874 3.1:+5071800 MS.gene56280:CDS
AAAATTTGTGTGCTCCAAAG+AGG 0.589431 3.1:+5070603 MS.gene56280:CDS
TCATCACCACCTCTGGGCAC+CGG 0.597509 3.1:+5070212 MS.gene56280:CDS
CCTTTCTCAAAGCAATGGGT+GGG 0.612770 3.1:+5071292 MS.gene56280:intron
ATGCATTGATCTACACGAAG+AGG 0.614015 3.1:+5071488 MS.gene56280:CDS
GAATATTACATCCAATCCAA+TGG 0.614280 3.1:+5070378 MS.gene56280:CDS
AAATTTGTGTGCTCCAAAGA+GGG 0.614397 3.1:+5070604 MS.gene56280:CDS
TAAGCAATGATGAAGTTCAA+GGG 0.614643 3.1:-5071570 None:intergenic
GAATTTGTGTCAAAACAAGC+TGG 0.617409 3.1:+5070889 MS.gene56280:CDS
CTTCACCATACCTTAGTGAG+TGG 0.628280 3.1:-5070975 None:intergenic
AGGTTTGCAAAGAGGCGCGA+CGG 0.630884 3.1:+5071921 MS.gene56280:CDS
TGCTGGCTTCTAGAAAGGGT+AGG 0.631004 3.1:+5071901 MS.gene56280:CDS
GAAAGGGTAGGTTTGCAAAG+AGG 0.637487 3.1:+5071913 MS.gene56280:CDS
GCTGGTGGAATAAACACTGT+TGG 0.638577 3.1:+5070907 MS.gene56280:CDS
TTTGCAAAGAGGCGCGACGG+TGG 0.639189 3.1:+5071924 MS.gene56280:CDS
TCAAACTCCATTTCCAAGTG+TGG 0.642402 3.1:-5070469 None:intergenic
ATATAAGGGAAAATGCCCTA+AGG 0.647376 3.1:+5071412 MS.gene56280:CDS
TTTGTGTCAAAACAAGCTGG+TGG 0.647892 3.1:+5070892 MS.gene56280:CDS
TGCTCCAAAGAGGGTAGACG+CGG 0.660484 3.1:+5070613 MS.gene56280:CDS
GTGAATGATATCGTGCAACA+GGG 0.667513 3.1:+5070421 MS.gene56280:CDS
GGCGACGGCAGAAGTGACGG+AGG 0.729705 3.1:+5071976 MS.gene56280:CDS
AAAATCTTACCGGTGCCCAG+AGG 0.742952 3.1:-5070221 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATGATAATATCATAGT+TGG - chr3.1:5071618-5071637 None:intergenic 15.0%
!! TATATGATAATATCATAGTT+GGG - chr3.1:5071617-5071636 None:intergenic 15.0%
!!! AATTTTATAATGAGTATAGT+AGG + chr3.1:5070512-5070531 MS.gene56280:CDS 15.0%
!! AATTGTTCATATCAATTTAG+AGG - chr3.1:5071751-5071770 None:intergenic 20.0%
!! ATATGATAATATCATAGTTG+GGG - chr3.1:5071616-5071635 None:intergenic 20.0%
!! ATTATAAAATTCATATGCCA+AGG - chr3.1:5070503-5070522 None:intergenic 20.0%
!! CAAATAATCATTGAAAAGAA+GGG - chr3.1:5071269-5071288 None:intergenic 20.0%
!! CACAAATGATAAATGATTAT+GGG + chr3.1:5071106-5071125 MS.gene56280:intron 20.0%
!! TATTCACATTATCTAGAAAA+TGG - chr3.1:5070363-5070382 None:intergenic 20.0%
!! TCAAATAATCATTGAAAAGA+AGG - chr3.1:5071270-5071289 None:intergenic 20.0%
!!! ACAAATTCATGAAAATCTTT+TGG - chr3.1:5070877-5070896 None:intergenic 20.0%
!!! AGCAAATAACAAACATATTT+TGG + chr3.1:5071076-5071095 MS.gene56280:intron 20.0%
!!! CACTTTGTATTTTATTTTAC+TGG - chr3.1:5071724-5071743 None:intergenic 20.0%
!!! CATGAATATTTGAAAGTATT+TGG + chr3.1:5070275-5070294 MS.gene56280:intron 20.0%
!!! GCAAATAACAAACATATTTT+GGG + chr3.1:5071077-5071096 MS.gene56280:intron 20.0%
! ACAATTGAACATGTATTCAA+AGG + chr3.1:5071765-5071784 MS.gene56280:intron 25.0%
! ACAGACAAACAATTGTTTAT+GGG + chr3.1:5071217-5071236 MS.gene56280:intron 25.0%
! ATAAATGATTATGGGTACTT+TGG + chr3.1:5071114-5071133 MS.gene56280:intron 25.0%
! ATCATTTATCATTTGTGCAT+CGG - chr3.1:5071103-5071122 None:intergenic 25.0%
! GCAAATAAAAGCAAAAAAGA+TGG + chr3.1:5070565-5070584 MS.gene56280:CDS 25.0%
! GCACAAATGATAAATGATTA+TGG + chr3.1:5071105-5071124 MS.gene56280:intron 25.0%
! TATGATAATATCATAGTTGG+GGG - chr3.1:5071615-5071634 None:intergenic 25.0%
! TGAAACTCATAAAAGAATGA+AGG - chr3.1:5071042-5071061 None:intergenic 25.0%
!! ATTCTTATTTGTCTTGAATG+TGG - chr3.1:5071160-5071179 None:intergenic 25.0%
!! ATTCTTTTATGAGTTTCAGA+TGG + chr3.1:5071044-5071063 MS.gene56280:intron 25.0%
!!! TTTTATTTGCATATTCTCGA+CGG - chr3.1:5070555-5070574 None:intergenic 25.0%
AAAAGACAACAAAGACATGA+AGG - chr3.1:5071830-5071849 None:intergenic 30.0%
AAATAAGAATTATCGTCCGT+TGG + chr3.1:5071170-5071189 MS.gene56280:intron 30.0%
AATAAGAATTATCGTCCGTT+GGG + chr3.1:5071171-5071190 MS.gene56280:intron 30.0%
AATGAGTATAGTAGGAAATG+TGG + chr3.1:5070520-5070539 MS.gene56280:CDS 30.0%
ACGTGATTACTTAACAAAAG+AGG + chr3.1:5070645-5070664 MS.gene56280:CDS 30.0%
ATAAGCAATGATGAAGTTCA+AGG - chr3.1:5071574-5071593 None:intergenic 30.0%
ATCATATAAACATACTCAGC+TGG - chr3.1:5070784-5070803 None:intergenic 30.0%
ATGATAATATCATAGTTGGG+GGG - chr3.1:5071614-5071633 None:intergenic 30.0%
ATTCATCAGCCAATACAATT+TGG - chr3.1:5070843-5070862 None:intergenic 30.0%
ATTGAAGAATTAGAATGCCA+AGG + chr3.1:5071799-5071818 MS.gene56280:CDS 30.0%
ATTTAGTAATGTACCACACT+TGG + chr3.1:5070456-5070475 MS.gene56280:CDS 30.0%
CACAGACAAACAATTGTTTA+TGG + chr3.1:5071216-5071235 MS.gene56280:intron 30.0%
CAGACAAACAATTGTTTATG+GGG + chr3.1:5071218-5071237 MS.gene56280:intron 30.0%
CATAAACAATTGTTTGTCTG+TGG - chr3.1:5071218-5071237 None:intergenic 30.0%
GAATATTACATCCAATCCAA+TGG + chr3.1:5070378-5070397 MS.gene56280:CDS 30.0%
GACAAAGAACAAAATCTTAC+CGG - chr3.1:5070234-5070253 None:intergenic 30.0%
TAAGCAATGATGAAGTTCAA+GGG - chr3.1:5071573-5071592 None:intergenic 30.0%
TAGAAAACATGAACGAGAAA+GGG + chr3.1:5071849-5071868 MS.gene56280:CDS 30.0%
TCATAATTTGAGCAAGACTT+TGG - chr3.1:5071666-5071685 None:intergenic 30.0%
TTAGAAAACATGAACGAGAA+AGG + chr3.1:5071848-5071867 MS.gene56280:CDS 30.0%
TTCATCAGCCAATACAATTT+GGG - chr3.1:5070842-5070861 None:intergenic 30.0%
TTGAACTTCATCATTGCTTA+TGG + chr3.1:5071573-5071592 MS.gene56280:CDS 30.0%
TTGAAGAATTAGAATGCCAA+GGG + chr3.1:5071800-5071819 MS.gene56280:CDS 30.0%
! GTATGGTTATTTCACTTGAT+CGG + chr3.1:5070701-5070720 MS.gene56280:CDS 30.0%
! TATAGTAGGAAATGTGGTTT+TGG + chr3.1:5070526-5070545 MS.gene56280:CDS 30.0%
! TGATCTGTTAGAATGGTTTT+AGG - chr3.1:5071324-5071343 None:intergenic 30.0%
!! CAAGGTTATTGACTTTGTAA+CGG + chr3.1:5070738-5070757 MS.gene56280:CDS 30.0%
!!! AAGAAGAATTTTTGAGTGGA+TGG + chr3.1:5071511-5071530 MS.gene56280:CDS 30.0%
!!! AGAAGAATTTTTGAGTGGAT+GGG + chr3.1:5071512-5071531 MS.gene56280:CDS 30.0%
AAAATTTGTGTGCTCCAAAG+AGG + chr3.1:5070603-5070622 MS.gene56280:CDS 35.0%
AAATTTGTGTGCTCCAAAGA+GGG + chr3.1:5070604-5070623 MS.gene56280:CDS 35.0%
ACAAGCATCCCAAATTGTAT+TGG + chr3.1:5070831-5070850 MS.gene56280:CDS 35.0%
ATAGATGATTCACATGCCTT+AGG - chr3.1:5071431-5071450 None:intergenic 35.0%
ATATAAGGGAAAATGCCCTA+AGG + chr3.1:5071412-5071431 MS.gene56280:CDS 35.0%
ATTGTGCACTTGGCATATAA+GGG + chr3.1:5071398-5071417 MS.gene56280:CDS 35.0%
ATTTCACTTGATCGGAAGAT+TGG + chr3.1:5070709-5070728 MS.gene56280:CDS 35.0%
CACAATCCATGAAATGTTTC+TGG - chr3.1:5071384-5071403 None:intergenic 35.0%
GAAACCTTTCTCAAAGCAAT+GGG + chr3.1:5071288-5071307 MS.gene56280:intron 35.0%
GAATTTGTGTCAAAACAAGC+TGG + chr3.1:5070889-5070908 MS.gene56280:CDS 35.0%
TAATGTACCACACTTGGAAA+TGG + chr3.1:5070462-5070481 MS.gene56280:CDS 35.0%
TAGATGATTCACATGCCTTA+GGG - chr3.1:5071430-5071449 None:intergenic 35.0%
TAGTGCGAAGCAAATTTCTA+TGG - chr3.1:5070948-5070967 None:intergenic 35.0%
TCGGAAGATTGGAAAATACA+AGG + chr3.1:5070720-5070739 MS.gene56280:CDS 35.0%
TGAAACCTTTCTCAAAGCAA+TGG + chr3.1:5071287-5071306 MS.gene56280:intron 35.0%
TGATAATATCATAGTTGGGG+GGG - chr3.1:5071613-5071632 None:intergenic 35.0%
TGCATCTTGATCTGTTAGAA+TGG - chr3.1:5071331-5071350 None:intergenic 35.0%
TTCATCATTGCTTATGGACT+TGG + chr3.1:5071579-5071598 MS.gene56280:CDS 35.0%
TTGTTTGTCTGTGGCTATTA+AGG - chr3.1:5071209-5071228 None:intergenic 35.0%
! GAAATTTGCTTCGCACTAAA+AGG + chr3.1:5070950-5070969 MS.gene56280:CDS 35.0%
! GGTTGAAGGTGTAGAAAAAT+TGG + chr3.1:5070399-5070418 MS.gene56280:CDS 35.0%
! TTTTCTACACCTTCAACCAT+TGG - chr3.1:5070397-5070416 None:intergenic 35.0%
!! ATTGTATTGGCTGATGAATC+TGG + chr3.1:5070844-5070863 MS.gene56280:CDS 35.0%
!! GAGGAAGAAGAATTTTTGAG+TGG + chr3.1:5071507-5071526 MS.gene56280:CDS 35.0%
AACAGATCAAGATGCAGCAA+TGG + chr3.1:5071335-5071354 MS.gene56280:CDS 40.0%
AACAGGATGTTTAGCACGTA+TGG + chr3.1:5070684-5070703 MS.gene56280:CDS 40.0%
ACATCCAATCCAATGGTTGA+AGG + chr3.1:5070385-5070404 MS.gene56280:CDS 40.0%
CTAAAAGGAACCACTCACTA+AGG + chr3.1:5070965-5070984 MS.gene56280:CDS 40.0%
GATTGTGCACTTGGCATATA+AGG + chr3.1:5071397-5071416 MS.gene56280:CDS 40.0%
GTCATGCCAGAAACATTTCA+TGG + chr3.1:5071375-5071394 MS.gene56280:CDS 40.0%
GTGAATGATATCGTGCAACA+GGG + chr3.1:5070421-5070440 MS.gene56280:CDS 40.0%
TACACCTTCAACCATTGGAT+TGG - chr3.1:5070392-5070411 None:intergenic 40.0%
TCAAACTCCATTTCCAAGTG+TGG - chr3.1:5070472-5070491 None:intergenic 40.0%
TCACTAAGGTATGGTGAAGT+AGG + chr3.1:5070979-5070998 MS.gene56280:CDS 40.0%
TTTGTGTCAAAACAAGCTGG+TGG + chr3.1:5070892-5070911 MS.gene56280:CDS 40.0%
! AAGACATGAAGGTTTTCCCT+TGG - chr3.1:5071819-5071838 None:intergenic 40.0%
! ATGCATTGATCTACACGAAG+AGG + chr3.1:5071488-5071507 MS.gene56280:CDS 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr3.1:5071388-5071407 MS.gene56280:CDS 40.0%
!!! AGAATTTTTGAGTGGATGGG+AGG + chr3.1:5071515-5071534 MS.gene56280:CDS 40.0%
ACCTTTCTCAAAGCAATGGG+TGG + chr3.1:5071291-5071310 MS.gene56280:intron 45.0%
AGGAACCACTCACTAAGGTA+TGG + chr3.1:5070970-5070989 MS.gene56280:CDS 45.0%
AGGCCAACAAATACTCCCAA+CGG - chr3.1:5071189-5071208 None:intergenic 45.0%
CCTTTCTCAAAGCAATGGGT+GGG + chr3.1:5071292-5071311 MS.gene56280:intron 45.0%
CTTCACCATACCTTAGTGAG+TGG - chr3.1:5070978-5070997 None:intergenic 45.0%
GAAAGGGTAGGTTTGCAAAG+AGG + chr3.1:5071913-5071932 MS.gene56280:CDS 45.0%
GAACGAGAAAGGGCAGATTT+GGG + chr3.1:5071859-5071878 MS.gene56280:CDS 45.0%
GCTGGTGGAATAAACACTGT+TGG + chr3.1:5070907-5070926 MS.gene56280:CDS 45.0%
GGAAATGGAGTTTGAGTCTG+AGG + chr3.1:5070477-5070496 MS.gene56280:CDS 45.0%
GGTGAATGATATCGTGCAAC+AGG + chr3.1:5070420-5070439 MS.gene56280:CDS 45.0%
GTCACAGTAACATATGGTGC+TGG + chr3.1:5071884-5071903 MS.gene56280:CDS 45.0%
TCAAGATGCAGCAATGGCTA+AGG + chr3.1:5071341-5071360 MS.gene56280:CDS 45.0%
TGAACGAGAAAGGGCAGATT+TGG + chr3.1:5071858-5071877 MS.gene56280:CDS 45.0%
TGGGTCGTCACAGTAACATA+TGG + chr3.1:5071878-5071897 MS.gene56280:CDS 45.0%
!! ATGGTGCTGGCTTCTAGAAA+GGG + chr3.1:5071897-5071916 MS.gene56280:CDS 45.0%
!! TATGGTGCTGGCTTCTAGAA+AGG + chr3.1:5071896-5071915 MS.gene56280:CDS 45.0%
AAAATCTTACCGGTGCCCAG+AGG - chr3.1:5070224-5070243 None:intergenic 50.0%
CACCATTCATCACCACCTCT+GGG + chr3.1:5070206-5070225 MS.gene56280:CDS 50.0%
CATACTCAGCTGGCAGAAGA+AGG - chr3.1:5070774-5070793 None:intergenic 50.0%
GCTCGAGCAGAAACTAGAAC+AGG + chr3.1:5070667-5070686 MS.gene56280:CDS 50.0%
TCACCATTCATCACCACCTC+TGG + chr3.1:5070205-5070224 MS.gene56280:CDS 50.0%
!! CCCACCCATTGCTTTGAGAA+AGG - chr3.1:5071295-5071314 None:intergenic 50.0%
!! CGTCCGTTGGGAGTATTTGT+TGG + chr3.1:5071183-5071202 MS.gene56280:intron 50.0%
!! GAGGGCTTTGCTTTGAGATG+CGG + chr3.1:5071955-5071974 MS.gene56280:CDS 50.0%
!! TGCTGGCTTCTAGAAAGGGT+AGG + chr3.1:5071901-5071920 MS.gene56280:CDS 50.0%
!! TTTGCTTTGAGATGCGGCGA+CGG + chr3.1:5071961-5071980 MS.gene56280:CDS 50.0%
TGCTCCAAAGAGGGTAGACG+CGG + chr3.1:5070613-5070632 MS.gene56280:CDS 55.0%
! AGGTTTGCAAAGAGGCGCGA+CGG + chr3.1:5071921-5071940 MS.gene56280:CDS 55.0%
! GGAGTTTGAGTCTGAGGCCT+TGG + chr3.1:5070483-5070502 MS.gene56280:CDS 55.0%
! TGCCCAGAGGTGGTGATGAA+TGG - chr3.1:5070211-5070230 None:intergenic 55.0%
ATCTTACCGGTGCCCAGAGG+TGG - chr3.1:5070221-5070240 None:intergenic 60.0%
GACACCGCGTCTACCCTCTT+TGG - chr3.1:5070620-5070639 None:intergenic 60.0%
TCATCACCACCTCTGGGCAC+CGG + chr3.1:5070212-5070231 MS.gene56280:CDS 60.0%
! TTTGCAAAGAGGCGCGACGG+TGG + chr3.1:5071924-5071943 MS.gene56280:CDS 60.0%
TGCGGCGACGGCAGAAGTGA+CGG + chr3.1:5071973-5071992 MS.gene56280:CDS 65.0%
GGCGACGGCAGAAGTGACGG+AGG + chr3.1:5071976-5071995 MS.gene56280:CDS 70.0%
! AAGAGGCGCGACGGTGGCAG+TGG + chr3.1:5071930-5071949 MS.gene56280:CDS 70.0%
AGGCGCGACGGTGGCAGTGG+CGG + chr3.1:5071933-5071952 MS.gene56280:CDS 75.0%
! GCGACGGTGGCAGTGGCGGA+GGG + chr3.1:5071937-5071956 MS.gene56280:CDS 75.0%
! CGCGACGGTGGCAGTGGCGG+AGG + chr3.1:5071936-5071955 MS.gene56280:CDS 80.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 5070167 5072005 5070167 ID=MS.gene56280
chr3.1 mRNA 5070167 5072005 5070167 ID=MS.gene56280.t1;Parent=MS.gene56280
chr3.1 exon 5070167 5070233 5070167 ID=MS.gene56280.t1.exon1;Parent=MS.gene56280.t1
chr3.1 CDS 5070167 5070233 5070167 ID=cds.MS.gene56280.t1;Parent=MS.gene56280.t1
chr3.1 exon 5070371 5071000 5070371 ID=MS.gene56280.t1.exon2;Parent=MS.gene56280.t1
chr3.1 CDS 5070371 5071000 5070371 ID=cds.MS.gene56280.t1;Parent=MS.gene56280.t1
chr3.1 exon 5071304 5071605 5071304 ID=MS.gene56280.t1.exon3;Parent=MS.gene56280.t1
chr3.1 CDS 5071304 5071605 5071304 ID=cds.MS.gene56280.t1;Parent=MS.gene56280.t1
chr3.1 exon 5071787 5072005 5071787 ID=MS.gene56280.t1.exon4;Parent=MS.gene56280.t1
chr3.1 CDS 5071787 5072005 5071787 ID=cds.MS.gene56280.t1;Parent=MS.gene56280.t1
Gene Sequence

>MS.gene56280

ATGGTAGAAACGACACACGTAGAACATGATGCAAATCTTCACCATTCATCACCACCTCTGGGCACCGGTAAGATTTTGTTCTTTGTCGATGAATATACACACGTAGAACATGAATATTTGAAAGTATTTGGACAATATAAAATTAATATAAAATGTCTATGAATATTAACATGTTGACTTTATATATTTTTTTCCATTTTCTAGATAATGTGAATATTACATCCAATCCAATGGTTGAAGGTGTAGAAAAATTGGTGAATGATATCGTGCAACAGGGAAAAGAGAGTACATTTAGTAATGTACCACACTTGGAAATGGAGTTTGAGTCTGAGGCCTTGGCATATGAATTTTATAATGAGTATAGTAGGAAATGTGGTTTTGGTATCCGTCGAGAATATGCAAATAAAAGCAAAAAAGATGGAGTTCTTACTTCAAGAAAATTTGTGTGCTCCAAAGAGGGTAGACGCGGTGTCAACAAACGTGATTACTTAACAAAAGAGGCTCGAGCAGAAACTAGAACAGGATGTTTAGCACGTATGGTTATTTCACTTGATCGGAAGATTGGAAAATACAAGGTTATTGACTTTGTAACGGAACATAATCACCTTCTTCTGCCAGCTGAGTATGTTTATATGATTCGCTCTCATCGACACATATCTGAGTCACAAGCATCCCAAATTGTATTGGCTGATGAATCTGGATTGAAACCAAAAGATTTTCATGAATTTGTGTCAAAACAAGCTGGTGGAATAAACACTGTTGGTTACACAAGACAAGACCATAGAAATTTGCTTCGCACTAAAAGGAACCACTCACTAAGGTATGGTGAAGTAGGTGCATTACTAATACATTTCAAAAAGCAAAGTGAAAATCCTTCATTCTTTTATGAGTTTCAGATGGATGTTGAAGAGCAAATAACAAACATATTTTGGGCCGATGCACAAATGATAAATGATTATGGGTACTTTGGTGATGTTGTCACTTTTGACACCACATTCAAGACAAATAAGAATTATCGTCCGTTGGGAGTATTTGTTGGCCTTAATAGCCACAGACAAACAATTGTTTATGGGGCAGCATTGTTATATGATGAAACAATCCCTTCTTTTCAATGATTATTTGAAACCTTTCTCAAAGCAATGGGTGGGAAAAAGCCTAAAACCATTCTAACAGATCAAGATGCAGCAATGGCTAAGGCTCTTTCTATAGTCATGCCAGAAACATTTCATGGATTGTGCACTTGGCATATAAGGGAAAATGCCCTAAGGCATGTGAATCATCTATATCAACAGAGCTCACAATTCTGCTCAGATTTTGAAGCATGCATTGATCTACACGAAGAGGAAGAAGAATTTTTGAGTGGATGGGAGGCTTTACTAGTTGAGCATAATGTTTCTAACTATCCCTTGAACTTCATCATTGCTTATGGACTTGGCTCAGTAACCCCCCCAACTATGATATTATCATATATGTATCAGCATTACATGTAACAGCAACCAAAGTCTTGCTCAAATTATGAATATCATCACTAGCAAAATCTGCAAAAAGAATAAGCCAGTAAAATAAAATACAAAGTGAGTTCCTCTAAATTGATATGAACAATTGAACATGTATTCAAAGGTATCTGTCAAAATTGAAGAATTAGAATGCCAAGGGAAAACCTTCATGTCTTTGTTGTCTTTTAGAAAACATGAACGAGAAAGGGCAGATTTGGGTCGTCACAGTAACATATGGTGCTGGCTTCTAGAAAGGGTAGGTTTGCAAAGAGGCGCGACGGTGGCAGTGGCGGAGGGCTTTGCTTTGAGATGCGGCGACGGCAGAAGTGACGGAGGTCGTTAA
Protein sequence

>MS.gene56280.t1

MVETTHVEHDANLHHSSPPLGTDNVNITSNPMVEGVEKLVNDIVQQGKESTFSNVPHLEMEFESEALAYEFYNEYSRKCGFGIRREYANKSKKDGVLTSRKFVCSKEGRRGVNKRDYLTKEARAETRTGCLARMVISLDRKIGKYKVIDFVTEHNHLLLPAEYVYMIRSHRHISESQASQIVLADESGLKPKDFHEFVSKQAGGINTVGYTRQDHRNLLRTKRNHSLRYGEVAMGGKKPKTILTDQDAAMAKALSIVMPETFHGLCTWHIRENALRHVNHLYQQSSQFCSDFEACIDLHEEEEEFLSGWEALLVEHNVSNYPLNFIIAYGLGSVSVKIEELECQGKTFMSLLSFRKHERERADLGRHSNIWCWLLERVGLQRGATVAVAEGFALRCGDGRSDGGR