AlfalfaGEDB | MS.gene56333

M. sativa cultivar XinJiangDaYe / MS.gene56333

KEGG: -
GO: GO:0003674 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914
PFAM: PF03101.18 PF04434.20 PF10551.12

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56333.t1	XP_014518279.1	51.7	766	335	5	39	778	27	783	5.20E-226	794.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56333.t1	Q9SWG3	32.0	734	458	15	48	771	51	753	1.3e-103	379.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56333.t1	A0A1S3VJK5	51.7	766	335	5	39	778	27	783	3.8e-226	794.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene56333.t1	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56333.t1	MTR_6g051425	30.325	277	176	5	50	313	19	291	3.26e-35	137
MS.gene56333.t1	MTR_0856s0010	44.654	159	85	2	16	173	4	160	1.47e-34	129
MS.gene56333.t1	MTR_4g035820	29.831	295	183	12	9	288	39	324	1.06e-22	103
MS.gene56333.t1	MTR_0558s0020	26.000	250	161	5	52	277	40	289	6.97e-21	94.4
MS.gene56333.t1	MTR_1g070355	28.936	235	152	7	54	278	80	309	1.61e-20	96.7
MS.gene56333.t1	MTR_7g446060	51.220	82	40	0	130	211	7	88	6.25e-20	87.0
MS.gene56333.t1	MTR_7g446520	28.151	238	154	5	52	286	92	315	2.07e-18	87.8
MS.gene56333.t1	MTR_6g022070	29.655	145	98	2	338	482	1	141	8.90e-17	82.8
MS.gene56333.t1	MTR_3g450850	25.904	332	219	11	8	320	9	332	1.24e-16	82.8
MS.gene56333.t1	MTR_7g446480	31.395	172	117	1	122	292	143	314	1.96e-16	81.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56333.t1	AT4G15090	31.967	732	461	14	48	771	51	753	2.15e-120	382
MS.gene56333.t1	AT4G15090	31.967	732	461	14	48	771	51	753	2.15e-120	382
MS.gene56333.t1	AT4G15090	31.967	732	461	14	48	771	51	753	2.15e-120	382
MS.gene56333.t1	AT4G15090	31.967	732	461	14	48	771	51	753	2.15e-120	382
MS.gene56333.t1	AT4G15090	31.967	732	461	14	48	771	51	753	2.15e-120	382
MS.gene56333.t1	AT4G38180	35.511	597	364	10	48	631	73	661	1.61e-115	368
MS.gene56333.t1	AT3G22170	31.644	651	386	11	18	643	35	651	1.08e-107	349
MS.gene56333.t1	AT3G22170	31.644	651	386	11	18	643	35	651	1.08e-107	349
MS.gene56333.t1	AT1G76320	32.027	587	375	9	52	633	1	568	6.73e-102	329
MS.gene56333.t1	AT1G76320	32.027	587	375	9	52	633	1	568	1.68e-101	330

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Find 156 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAAATGTTTCGGTCG+TGG	0.509616	3.1:+6394935	MS.gene56333:CDS
AAAACCACTAGTTGACTGTT+GGG	0.492621	3.1:-6395133	None:intergenic
AAAACTAGAAACTCTAAGAA+AGG	0.460549	3.1:+6392971	MS.gene56333:CDS
AAACGATCAGGCAGGTGTAC+GGG	0.512319	3.1:+6393252	MS.gene56333:CDS
AAACTAGAAACTCTAAGAAA+GGG	0.523644	3.1:+6392972	MS.gene56333:CDS
AAAGGATGATCGATCAATAT+AGG	0.415391	3.1:-6392761	None:intergenic
AAAGGATGCGCACAATACAA+AGG	0.625240	3.1:+6393844	MS.gene56333:CDS
AAATGCAACTGTTATCTCGA+AGG	0.615193	3.1:+6394297	MS.gene56333:CDS
AAATGGTCTAGCAAGAAGTT+GGG	0.529100	3.1:+6394717	MS.gene56333:CDS
AACATCACCAAAGTATTCAT+AGG	0.473828	3.1:-6393524	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGATGATCGATCAATAT+AGG	-	chr3.1:6392764-6392783	None:intergenic	30.0%
ACACAATACTTAGTTTCGTT+CGG	-	chr3.1:6394387-6394406	None:intergenic	30.0%
AGATAAGTCCAACTAAATCA+CGG	+	chr3.1:6393176-6393195	MS.gene56333:CDS	30.0%
AGATACAAGTAGAATTTCGT+GGG	+	chr3.1:6394274-6394293	MS.gene56333:CDS	30.0%
ATATTTGTTTGTCAAGTAGG+TGG	-	chr3.1:6393563-6393582	None:intergenic	30.0%
CATTTCTTCATACAAATCAG+AGG	-	chr3.1:6394694-6394713	None:intergenic	30.0%
GTCATATTTGTTTGTCAAGT+AGG	-	chr3.1:6393566-6393585	None:intergenic	30.0%
TTAAAAGACATACCGATAGT+AGG	-	chr3.1:6392890-6392909	None:intergenic	30.0%
TTATGAAATTGCAGAATGTG+CGG	+	chr3.1:6394650-6394669	MS.gene56333:CDS	30.0%
AAAACCACTAGTTGACTGTT+GGG	-	chr3.1:6395136-6395155	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	6392746	6395206	6392746	ID=MS.gene56333
chr3.1	mRNA	6392746	6395206	6392746	ID=MS.gene56333.t1;Parent=MS.gene56333
chr3.1	exon	6392746	6394959	6392746	ID=MS.gene56333.t1.exon1;Parent=MS.gene56333.t1
chr3.1	CDS	6392746	6394959	6392746	ID=cds.MS.gene56333.t1;Parent=MS.gene56333.t1
chr3.1	exon	6395069	6395206	6395069	ID=MS.gene56333.t1.exon2;Parent=MS.gene56333.t1
chr3.1	CDS	6395069	6395206	6395069	ID=cds.MS.gene56333.t1;Parent=MS.gene56333.t1

Gene Sequence

>MS.gene56333

ATGGCTGACTGTCCTCCTATATTGATCGATCATCCTTTATTAGCCCCTAATGGAGAGCTAGATGACGGTGCTGCTCGTGTTGACGTTTGGGATTCCGGGACCTTTGAAACAACAAATGATGTCGCCACAGATAAATTCACGCCTACTATCGGTATGTCTTTTAAATCGGTTGCAGATTGTAAGTCTTTTTACCGTGAATATGCCATGAAACAAGGGTTTGCGATCAAAACTAGAAACTCTAAGAAAGGGTCTGATGGCATGTTAAGATATTTCATTCTAACTTGTGCAAGAGAAGGTCACCATGAATCAAACATACCACCAACTTTGAAGACGAATCCAACGAAGAAACATGATTGCTCAGCTAAGATAACGGTTGCTTTAAAGAAGGGTCTATGGACTATTTTGTCTTTTAATTCATGTCATAATCATGAGATAAGTCCAACTAAATCACGGTTGTTTGCTGGCAATAAGAAGATTGATATGCATGCACAGCGGACAATTTTGATAAACGATCAGGCAGGTGTACGGGTGAACAAAAGTTTCCGTTCCATGGTATGTGATGCCGGTGGTTACGAAAATCTAACATTTGTGGAGAGAGATGTCAGAAACTTTGTGGCAAAAGAAAGACACCTTCTCGGGAAGGAAGGAGATGGCAGGGCACTTTTATCCTACTTTGCTAGAATGAGGGAATGCAACAAAGACTTTTTCTATGAGATTGACATGGACGAGGACTTTCATGTGCAGAATATTTTCTGGGCTGATGCACGTAGTAAGTCTGCCTATGAATACTTTGGTGATGTTGTCACGTTCGATACCACCTACTTGACAAACAAATATGACATGTCATTTGCCCCGTTTGTTGGTGTCAATCATCATGGGCAATCCATTTTACTAGGTTGCGGTCTTTTATCAGCTGAAGATACTAGGTCCTTTACCTGGTTGTTCGTAACATGGCTAAGGTGTATGTCTTCGAGGCCTCCAATGGGAATTGTAACCGATCAGTGCAAGGCTATGCAAAATGCAATTCAGGTTGTGTTTCCTAATACTAGGCATAGGTGGTGTCTATGGCATATAATGAGAAAAATTGCCGAAAAATTGAAAGGATGCGCACAATACAAAGGAATAAAAAATGATATGAAAAATTGTGTGTATGATTCTCTGCGGGTTGATGATTTTGTTGTTGGGTGGACAACATTCATTGAAAAATATGGTTTGGGTGAAAATGATTGGTTGAATACCATTTTCGAAGAGCGGGAAAGATGGGTGCCATGTTATTTAAAACATGATTTCTGGGCCGGAATGTCAACAACAAAGAGGAGTGAAAGTATGAATGCTTTTTTCGATGGTTACATTAATGCAAGAACTTCTCTCAGCGAATTTGTAAAGCAATACGACAACGCACTCCAAGACAAGGCTGAAAAGGAATACGAGGCAGATTTTCGGTCTTCAAGCACAATCATTCCATGCGGGTCAAATTCCACAATCGAGAGACAATTTCAAGCCGAGTACACCCATGAAAAGTATATGGAGATACAAGTAGAATTTCGTGGGAAATGCAACTGTTATCTCGAAGGTGTGACTACATTGGAAATTACATCTACATACACCGTTTTAGAGGAGAGTATTGTCTGTGGTAAACCGAACGAAACTAAGTATTGTGTTGATTTCAACGGGGAAGACAGTAATGTTACGTGCACTTGTTTGATGTTTGAGTTCAGAGGCATTTTGTGTAGACATTCGCTTTTCGTACTTGGTCATCTTAGAGTGAAAGAGGTTCCGTCTAAATTTATCTTGCCACGATGGAGTAAAAATGTGAAGAGAAAATATGCACACATGAAGACAAGTTACAATGCCAAAAAACTACAACCACACATAGAAAGATTTGAAGGTCTTTGCAAGACATTTTATGAAATTGCAGAATGTGCGGCTGAGACACCTAGTCAGACCTCTGATTTGTATGAAGAAATGATCAAATGGTCTAGCAAGAAGTTGGGCAAAATTAGTTTACAGCCACATTGTGGTGTGGGAGGGGATGATAGCTTAAACACGATGGAGGACTCATTATCACAGCAACCTGATCAACCAATTCTTAATCCGTTAAAAGTACCACGTACGGGACGTCTTCGAACTAATCGTCGGTTGTCCACCACTGAAAAGATTGTGAAAAAAAGAAGCAAAATGCAAAGAAAAAAACCAAATGTTTCGGTCGTGGAAGTAAGTTGGTGTTAACCTATTATTATTGTGATTACATTTGTTTTTTTTAATAGTAATACAGTGTTAATTATGTTAAATGTAATTACTATTTCACTCTGTAATACTACAGCCTACATTACTTCCTATGTCTGATGGGTTTCAAGCGTACGGTGATACGCTTAACTACTTCTCATCCCAACAGTCAACTAGTGGTTTTATGTCGTTACTTGCATCAATGGGGAAAGATGTGGACGCAGCTGAATTTTAA

Protein sequence

>MS.gene56333.t1

MADCPPILIDHPLLAPNGELDDGAARVDVWDSGTFETTNDVATDKFTPTIGMSFKSVADCKSFYREYAMKQGFAIKTRNSKKGSDGMLRYFILTCAREGHHESNIPPTLKTNPTKKHDCSAKITVALKKGLWTILSFNSCHNHEISPTKSRLFAGNKKIDMHAQRTILINDQAGVRVNKSFRSMVCDAGGYENLTFVERDVRNFVAKERHLLGKEGDGRALLSYFARMRECNKDFFYEIDMDEDFHVQNIFWADARSKSAYEYFGDVVTFDTTYLTNKYDMSFAPFVGVNHHGQSILLGCGLLSAEDTRSFTWLFVTWLRCMSSRPPMGIVTDQCKAMQNAIQVVFPNTRHRWCLWHIMRKIAEKLKGCAQYKGIKNDMKNCVYDSLRVDDFVVGWTTFIEKYGLGENDWLNTIFEERERWVPCYLKHDFWAGMSTTKRSESMNAFFDGYINARTSLSEFVKQYDNALQDKAEKEYEADFRSSSTIIPCGSNSTIERQFQAEYTHEKYMEIQVEFRGKCNCYLEGVTTLEITSTYTVLEESIVCGKPNETKYCVDFNGEDSNVTCTCLMFEFRGILCRHSLFVLGHLRVKEVPSKFILPRWSKNVKRKYAHMKTSYNAKKLQPHIERFEGLCKTFYEIAECAAETPSQTSDLYEEMIKWSSKKLGKISLQPHCGVGGDDSLNTMEDSLSQQPDQPILNPLKVPRTGRLRTNRRLSTTEKIVKKRSKMQRKKPNVSVVEPTLLPMSDGFQAYGDTLNYFSSQQSTSGFMSLLASMGKDVDAAEF