Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56385.t1 | AES63738.2 | 81.7 | 120 | 22 | 0 | 1 | 120 | 1 | 120 | 3.20E-50 | 207.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56385.t1 | G7INX6 | 81.7 | 120 | 22 | 0 | 1 | 120 | 1 | 120 | 2.3e-50 | 207.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56385.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56385.t1 | MTR_2g012700 | 81.667 | 120 | 22 | 0 | 1 | 120 | 1 | 120 | 1.25e-68 | 202 |
| MS.gene56385.t1 | MTR_2g062330 | 79.661 | 118 | 23 | 1 | 3 | 120 | 23 | 139 | 3.64e-64 | 191 |
| MS.gene56385.t1 | MTR_7g117590 | 33.846 | 130 | 67 | 5 | 7 | 119 | 44 | 171 | 9.63e-18 | 74.7 |
| MS.gene56385.t1 | MTR_7g117585 | 39.744 | 78 | 47 | 0 | 38 | 115 | 64 | 141 | 2.40e-17 | 73.2 |
| MS.gene56385.t1 | MTR_5g071410 | 38.824 | 85 | 52 | 0 | 31 | 115 | 180 | 264 | 1.62e-16 | 73.2 |
| MS.gene56385.t1 | MTR_0010s0310 | 37.755 | 98 | 59 | 1 | 19 | 114 | 147 | 244 | 1.32e-15 | 70.5 |
| MS.gene56385.t1 | MTR_3g008190 | 27.835 | 97 | 70 | 0 | 24 | 120 | 6 | 102 | 1.95e-14 | 64.3 |
| MS.gene56385.t1 | MTR_6g477950 | 33.333 | 81 | 54 | 0 | 39 | 119 | 64 | 144 | 2.15e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTCAACGGTCCTGAATT+AGG | 0.221297 | 3.1:+7523007 | MS.gene56385:CDS |
| AAAATGCCGATGTTCATTAT+TGG | 0.252563 | 3.1:+7523038 | MS.gene56385:CDS |
| TACCAGTTTCTTCGTCAAAA+AGG | 0.277417 | 3.1:-7523259 | None:intergenic |
| TAATGTTATTGCTTCTACTT+TGG | 0.311699 | 3.1:+7523066 | MS.gene56385:CDS |
| GTAGCATACATCCTATTAAA+AGG | 0.335252 | 3.1:-7523413 | None:intergenic |
| TTTGGATGGAAACAATGTTT+TGG | 0.360997 | 3.1:+7523084 | MS.gene56385:CDS |
| GTACGGCTTTGTTCTTGAAA+AGG | 0.418940 | 3.1:+7523349 | MS.gene56385:CDS |
| AAGTCAACGGTCCTGAATTA+GGG | 0.429565 | 3.1:+7523008 | MS.gene56385:CDS |
| GTTATTGCTTCTACTTTGGA+TGG | 0.442931 | 3.1:+7523070 | MS.gene56385:CDS |
| TACGGCTTTGTTCTTGAAAA+GGG | 0.445793 | 3.1:+7523350 | MS.gene56385:CDS |
| CTTGAAAAGGGCCTAAAAGT+TGG | 0.503487 | 3.1:+7523362 | MS.gene56385:CDS |
| AGCATGCATCAGAATTACAT+AGG | 0.573184 | 3.1:+7523317 | MS.gene56385:CDS |
| AGAATTACATAGGGAGAGGA+TGG | 0.573439 | 3.1:+7523327 | MS.gene56385:CDS |
| CATCAGAATTACATAGGGAG+AGG | 0.577691 | 3.1:+7523323 | MS.gene56385:CDS |
| GCATGCATCAGAATTACATA+GGG | 0.596673 | 3.1:+7523318 | MS.gene56385:CDS |
| TACATAGGGAGAGGATGGTA+CGG | 0.597869 | 3.1:+7523332 | MS.gene56385:CDS |
| ATACTTCCAATAATGAACAT+CGG | 0.656056 | 3.1:-7523044 | None:intergenic |
| ACATTCTCAGCAACACCTCG+AGG | 0.684927 | 3.1:-7523209 | None:intergenic |
| GAGACTGGAGTTGAAGTCAA+CGG | 0.686388 | 3.1:+7522995 | MS.gene56385:CDS |
| GAATTAGTCTCAGTTAACGA+CGG | 0.726011 | 3.1:-7523435 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACTTCCAATAATGAACAT+CGG | - | chr3.1:7523047-7523066 | None:intergenic | 25.0% |
| ! | TAATGTTATTGCTTCTACTT+TGG | + | chr3.1:7523066-7523085 | MS.gene56385:CDS | 25.0% |
| !! | AAATAATGTCACCAACTTTT+AGG | - | chr3.1:7523376-7523395 | None:intergenic | 25.0% |
| !! | CAGTCAATAATCCTTTTAAT+AGG | + | chr3.1:7523402-7523421 | MS.gene56385:CDS | 25.0% |
| AAAATGCCGATGTTCATTAT+TGG | + | chr3.1:7523038-7523057 | MS.gene56385:CDS | 30.0% | |
| GTAGCATACATCCTATTAAA+AGG | - | chr3.1:7523416-7523435 | None:intergenic | 30.0% | |
| ! | TTTGGATGGAAACAATGTTT+TGG | + | chr3.1:7523084-7523103 | MS.gene56385:CDS | 30.0% |
| ! | TTTTGAACATTCCCTAATTC+AGG | - | chr3.1:7523022-7523041 | None:intergenic | 30.0% |
| AGCATGCATCAGAATTACAT+AGG | + | chr3.1:7523317-7523336 | MS.gene56385:CDS | 35.0% | |
| GCATGCATCAGAATTACATA+GGG | + | chr3.1:7523318-7523337 | MS.gene56385:CDS | 35.0% | |
| GTTATTGCTTCTACTTTGGA+TGG | + | chr3.1:7523070-7523089 | MS.gene56385:CDS | 35.0% | |
| TACCAGTTTCTTCGTCAAAA+AGG | - | chr3.1:7523262-7523281 | None:intergenic | 35.0% | |
| !! | TACGGCTTTGTTCTTGAAAA+GGG | + | chr3.1:7523350-7523369 | MS.gene56385:CDS | 35.0% |
| AAGTCAACGGTCCTGAATTA+GGG | + | chr3.1:7523008-7523027 | MS.gene56385:CDS | 40.0% | |
| AGAATTACATAGGGAGAGGA+TGG | + | chr3.1:7523327-7523346 | MS.gene56385:CDS | 40.0% | |
| CATCAGAATTACATAGGGAG+AGG | + | chr3.1:7523323-7523342 | MS.gene56385:CDS | 40.0% | |
| CTTGAAAAGGGCCTAAAAGT+TGG | + | chr3.1:7523362-7523381 | MS.gene56385:CDS | 40.0% | |
| ! | CTATTGTAGCATTTTCCTCG+AGG | + | chr3.1:7523194-7523213 | MS.gene56385:intron | 40.0% |
| ! | CTCCTTTTTGACGAAGAAAC+TGG | + | chr3.1:7523257-7523276 | MS.gene56385:CDS | 40.0% |
| !! | GTACGGCTTTGTTCTTGAAA+AGG | + | chr3.1:7523349-7523368 | MS.gene56385:CDS | 40.0% |
| GAAGTCAACGGTCCTGAATT+AGG | + | chr3.1:7523007-7523026 | MS.gene56385:CDS | 45.0% | |
| GAGACTGGAGTTGAAGTCAA+CGG | + | chr3.1:7522995-7523014 | MS.gene56385:CDS | 45.0% | |
| TACATAGGGAGAGGATGGTA+CGG | + | chr3.1:7523332-7523351 | MS.gene56385:CDS | 45.0% | |
| ACATTCTCAGCAACACCTCG+AGG | - | chr3.1:7523212-7523231 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 7522989 | 7523448 | 7522989 | ID=MS.gene56385 |
| chr3.1 | mRNA | 7522989 | 7523448 | 7522989 | ID=MS.gene56385.t1;Parent=MS.gene56385 |
| chr3.1 | exon | 7522989 | 7523105 | 7522989 | ID=MS.gene56385.t1.exon1;Parent=MS.gene56385.t1 |
| chr3.1 | CDS | 7522989 | 7523105 | 7522989 | ID=cds.MS.gene56385.t1;Parent=MS.gene56385.t1 |
| chr3.1 | exon | 7523203 | 7523448 | 7523203 | ID=MS.gene56385.t1.exon2;Parent=MS.gene56385.t1 |
| chr3.1 | CDS | 7523203 | 7523448 | 7523203 | ID=cds.MS.gene56385.t1;Parent=MS.gene56385.t1 |
| Gene Sequence |
| Protein sequence |
