Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56450.t1 | AES90030.2 | 86.5 | 156 | 18 | 1 | 10 | 162 | 38 | 193 | 5.40E-69 | 270.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56450.t1 | Q7XLP4 | 39.1 | 110 | 63 | 2 | 34 | 140 | 83 | 191 | 7.0e-17 | 88.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56450.t1 | G7JHL3 | 86.5 | 156 | 18 | 1 | 10 | 162 | 38 | 193 | 3.9e-69 | 270.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56450.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56450.t1 | MTR_4g083390 | 86.538 | 156 | 18 | 1 | 10 | 162 | 38 | 193 | 2.70e-95 | 274 |
| MS.gene56450.t1 | MTR_4g083390 | 90.977 | 133 | 12 | 0 | 30 | 162 | 1 | 133 | 3.90e-88 | 253 |
| MS.gene56450.t1 | MTR_4g083390 | 85.496 | 131 | 19 | 0 | 30 | 160 | 1 | 131 | 2.63e-80 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAATTGAACCGCTTTC+AGG | 0.230161 | 4.4:+29413281 | MS.gene56450:CDS |
| TTCAAAGTTCAAATTCTTAG+AGG | 0.303256 | 4.4:+29413691 | MS.gene56450:CDS |
| AGTTGGGCCACGACTTATAA+TGG | 0.308961 | 4.4:+29413547 | MS.gene56450:CDS |
| ACGGGGACTAAGATGTGTTT+TGG | 0.312922 | 4.4:-29413330 | None:intergenic |
| TTCCGACTATTCTCAAATAT+CGG | 0.320685 | 4.4:+29413518 | MS.gene56450:CDS |
| GCATCCCTCTCACCGGTTCC+AGG | 0.356774 | 4.4:-29413745 | None:intergenic |
| TGAAATTACCAATCCATAAC+GGG | 0.384987 | 4.4:-29413348 | None:intergenic |
| GAGAATAGTCGGAACCGATT+TGG | 0.387243 | 4.4:-29413509 | None:intergenic |
| ACACATCTTAGTCCCCGTTA+TGG | 0.404304 | 4.4:+29413335 | MS.gene56450:CDS |
| GAAGATAATTATTTGAAGCT+TGG | 0.405476 | 4.4:+29413619 | MS.gene56450:CDS |
| TCCGACTATTCTCAAATATC+GGG | 0.408506 | 4.4:+29413519 | MS.gene56450:CDS |
| GCCAACGCTATCAAACAGTT+TGG | 0.414501 | 4.4:-29413581 | None:intergenic |
| AACTGTTTGATAGCGTTGGC+TGG | 0.415821 | 4.4:+29413584 | MS.gene56450:CDS |
| GAAAGGACCACATGAAAGTA+TGG | 0.437117 | 4.4:-29413308 | None:intergenic |
| CTTTACGAATTTCCTGGAAC+CGG | 0.450094 | 4.4:+29413733 | MS.gene56450:CDS |
| CAAATATCGGGGTAAGAGTT+GGG | 0.464634 | 4.4:+29413531 | MS.gene56450:CDS |
| GATTACTAATCTATGACAAA+TGG | 0.467072 | 4.4:-29413769 | None:intergenic |
| TCAAATATCGGGGTAAGAGT+TGG | 0.468409 | 4.4:+29413530 | MS.gene56450:CDS |
| TGAAGAACTTCCTTCCAAAT+CGG | 0.469161 | 4.4:+29413495 | MS.gene56450:CDS |
| GTTGGGCCACGACTTATAAT+GGG | 0.482324 | 4.4:+29413548 | MS.gene56450:CDS |
| ATGAAATTACCAATCCATAA+CGG | 0.499697 | 4.4:-29413349 | None:intergenic |
| ATGTCGTCTTCAAAATCAGC+TGG | 0.502794 | 4.4:+29413096 | MS.gene56450:CDS |
| ACCAAACTGTTTGATAGCGT+TGG | 0.508522 | 4.4:+29413580 | MS.gene56450:CDS |
| CCCCGATATTTGAGAATAGT+CGG | 0.515602 | 4.4:-29413520 | None:intergenic |
| TCTTAGTCCCCGTTATGGAT+TGG | 0.515821 | 4.4:+29413340 | MS.gene56450:CDS |
| ACCTTCGACAAATATGCAAA+CGG | 0.530039 | 4.4:+29413259 | MS.gene56450:CDS |
| ACTGTTTGATAGCGTTGGCT+GGG | 0.539366 | 4.4:+29413585 | MS.gene56450:CDS |
| GAAGGTGATGATAACGAGGC+TGG | 0.539673 | 4.4:-29413242 | None:intergenic |
| TCCGTTTGCATATTTGTCGA+AGG | 0.549182 | 4.4:-29413260 | None:intergenic |
| GTTCTGCCCATTATAAGTCG+TGG | 0.557426 | 4.4:-29413554 | None:intergenic |
| TCTTCAAAATCAGCTGGAAG+AGG | 0.564849 | 4.4:+29413102 | MS.gene56450:CDS |
| AAATGGTGCATCCCTCTCAC+CGG | 0.578086 | 4.4:-29413752 | None:intergenic |
| AGGAGTGCCATACTTTCATG+TGG | 0.578309 | 4.4:+29413301 | MS.gene56450:CDS |
| TCGACAAATATGCAAACGGA+TGG | 0.583245 | 4.4:+29413263 | MS.gene56450:CDS |
| GAGTAGCAGTCAAGTTGCTG+TGG | 0.583739 | 4.4:+29413214 | MS.gene56450:CDS |
| TCTTCACATATGCTGCTGCT+CGG | 0.585483 | 4.4:-29413478 | None:intergenic |
| ATTTCCTGGAACCGGTGAGA+GGG | 0.587927 | 4.4:+29413741 | MS.gene56450:CDS |
| AGTAGCAGTCAAGTTGCTGT+GGG | 0.603556 | 4.4:+29413215 | MS.gene56450:CDS |
| AATTTCCTGGAACCGGTGAG+AGG | 0.633829 | 4.4:+29413740 | MS.gene56450:CDS |
| ATAGTCGGAACCGATTTGGA+AGG | 0.643344 | 4.4:-29413505 | None:intergenic |
| AAGTATGGCACTCCTGAAAG+CGG | 0.678549 | 4.4:-29413293 | None:intergenic |
| CCGACTATTCTCAAATATCG+GGG | 0.681942 | 4.4:+29413520 | MS.gene56450:CDS |
| GAAATTACCAATCCATAACG+GGG | 0.716232 | 4.4:-29413347 | None:intergenic |
| TGTCGAAGGTGATGATAACG+AGG | 0.728967 | 4.4:-29413246 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGTGATATGAAATACTA+AGG | - | chr4.4:29413135-29413154 | None:intergenic | 20.0% |
| !! | TTATAGATCAAACTAAATGA+AGG | + | chr4.4:29413389-29413408 | MS.gene56450:intron | 20.0% |
| ! | AAGATTAACTTTCATGTACA+CGG | + | chr4.4:29413157-29413176 | MS.gene56450:intron | 25.0% |
| ! | ATGAAATTACCAATCCATAA+CGG | - | chr4.4:29413352-29413371 | None:intergenic | 25.0% |
| ! | CTTGTATTGTATTGTATTGT+TGG | + | chr4.4:29413445-29413464 | MS.gene56450:intron | 25.0% |
| ! | TATTGTATTGTTGGCATATA+GGG | + | chr4.4:29413454-29413473 | MS.gene56450:intron | 25.0% |
| ! | TTCAAAGTTCAAATTCTTAG+AGG | + | chr4.4:29413691-29413710 | MS.gene56450:CDS | 25.0% |
| !! | ATCAAACTAAATGAAGGTTT+TGG | + | chr4.4:29413395-29413414 | MS.gene56450:intron | 25.0% |
| !! | GAAGATAATTATTTGAAGCT+TGG | + | chr4.4:29413619-29413638 | MS.gene56450:CDS | 25.0% |
| ATTGTATTGTTGGCATATAG+GGG | + | chr4.4:29413455-29413474 | MS.gene56450:intron | 30.0% | |
| GGAAATTCGTAAAGCAAAAA+TGG | - | chr4.4:29413727-29413746 | None:intergenic | 30.0% | |
| GTATTGTATTGTTGGCATAT+AGG | + | chr4.4:29413453-29413472 | MS.gene56450:intron | 30.0% | |
| GTGTACATGAAAGTTAATCT+TGG | - | chr4.4:29413158-29413177 | None:intergenic | 30.0% | |
| TGAAATTACCAATCCATAAC+GGG | - | chr4.4:29413351-29413370 | None:intergenic | 30.0% | |
| TTCCGACTATTCTCAAATAT+CGG | + | chr4.4:29413518-29413537 | MS.gene56450:CDS | 30.0% | |
| ! | TTTTTGCTTTACGAATTTCC+TGG | + | chr4.4:29413727-29413746 | MS.gene56450:CDS | 30.0% |
| !!! | ACTAAATGAAGGTTTTGGAA+AGG | + | chr4.4:29413400-29413419 | MS.gene56450:intron | 30.0% |
| ACCTTCGACAAATATGCAAA+CGG | + | chr4.4:29413259-29413278 | MS.gene56450:CDS | 35.0% | |
| GAAATTACCAATCCATAACG+GGG | - | chr4.4:29413350-29413369 | None:intergenic | 35.0% | |
| TCCGACTATTCTCAAATATC+GGG | + | chr4.4:29413519-29413538 | MS.gene56450:CDS | 35.0% | |
| TGAAGAACTTCCTTCCAAAT+CGG | + | chr4.4:29413495-29413514 | MS.gene56450:CDS | 35.0% | |
| TTAACTTTCATGTACACGGT+CGG | + | chr4.4:29413161-29413180 | MS.gene56450:intron | 35.0% | |
| !!! | GACTAAGATGTGTTTTGGAA+AGG | - | chr4.4:29413328-29413347 | None:intergenic | 35.0% |
| ATGTCGTCTTCAAAATCAGC+TGG | + | chr4.4:29413096-29413115 | MS.gene56450:CDS | 40.0% | |
| CAAATATCGGGGTAAGAGTT+GGG | + | chr4.4:29413531-29413550 | MS.gene56450:CDS | 40.0% | |
| CCCCGATATTTGAGAATAGT+CGG | - | chr4.4:29413523-29413542 | None:intergenic | 40.0% | |
| CCGACTATTCTCAAATATCG+GGG | + | chr4.4:29413520-29413539 | MS.gene56450:CDS | 40.0% | |
| CTTTACGAATTTCCTGGAAC+CGG | + | chr4.4:29413733-29413752 | MS.gene56450:CDS | 40.0% | |
| GAAAGGACCACATGAAAGTA+TGG | - | chr4.4:29413311-29413330 | None:intergenic | 40.0% | |
| GCTCATGAAAAACAGTGAAG+AGG | + | chr4.4:29413660-29413679 | MS.gene56450:CDS | 40.0% | |
| TCAAATATCGGGGTAAGAGT+TGG | + | chr4.4:29413530-29413549 | MS.gene56450:CDS | 40.0% | |
| TCCGTTTGCATATTTGTCGA+AGG | - | chr4.4:29413263-29413282 | None:intergenic | 40.0% | |
| TCGACAAATATGCAAACGGA+TGG | + | chr4.4:29413263-29413282 | MS.gene56450:CDS | 40.0% | |
| TCTTCAAAATCAGCTGGAAG+AGG | + | chr4.4:29413102-29413121 | MS.gene56450:CDS | 40.0% | |
| !! | ACCAAACTGTTTGATAGCGT+TGG | + | chr4.4:29413580-29413599 | MS.gene56450:CDS | 40.0% |
| AAGTATGGCACTCCTGAAAG+CGG | - | chr4.4:29413296-29413315 | None:intergenic | 45.0% | |
| ACACATCTTAGTCCCCGTTA+TGG | + | chr4.4:29413335-29413354 | MS.gene56450:CDS | 45.0% | |
| AGTTGGGCCACGACTTATAA+TGG | + | chr4.4:29413547-29413566 | MS.gene56450:CDS | 45.0% | |
| GAGAATAGTCGGAACCGATT+TGG | - | chr4.4:29413512-29413531 | None:intergenic | 45.0% | |
| GATGGAATTGAACCGCTTTC+AGG | + | chr4.4:29413281-29413300 | MS.gene56450:CDS | 45.0% | |
| GCCAACGCTATCAAACAGTT+TGG | - | chr4.4:29413584-29413603 | None:intergenic | 45.0% | |
| GTTCTGCCCATTATAAGTCG+TGG | - | chr4.4:29413557-29413576 | None:intergenic | 45.0% | |
| GTTGGGCCACGACTTATAAT+GGG | + | chr4.4:29413548-29413567 | MS.gene56450:CDS | 45.0% | |
| TCTTAGTCCCCGTTATGGAT+TGG | + | chr4.4:29413340-29413359 | MS.gene56450:CDS | 45.0% | |
| TCTTCACATATGCTGCTGCT+CGG | - | chr4.4:29413481-29413500 | None:intergenic | 45.0% | |
| ! | ACGGGGACTAAGATGTGTTT+TGG | - | chr4.4:29413333-29413352 | None:intergenic | 45.0% |
| ! | AGTAGCAGTCAAGTTGCTGT+GGG | + | chr4.4:29413215-29413234 | MS.gene56450:CDS | 45.0% |
| ! | TGTCGAAGGTGATGATAACG+AGG | - | chr4.4:29413249-29413268 | None:intergenic | 45.0% |
| !! | AACTGTTTGATAGCGTTGGC+TGG | + | chr4.4:29413584-29413603 | MS.gene56450:CDS | 45.0% |
| !! | ACTGTTTGATAGCGTTGGCT+GGG | + | chr4.4:29413585-29413604 | MS.gene56450:CDS | 45.0% |
| !! | AGGAGTGCCATACTTTCATG+TGG | + | chr4.4:29413301-29413320 | MS.gene56450:CDS | 45.0% |
| !! | ATAGTCGGAACCGATTTGGA+AGG | - | chr4.4:29413508-29413527 | None:intergenic | 45.0% |
| AAATGGTGCATCCCTCTCAC+CGG | - | chr4.4:29413755-29413774 | None:intergenic | 50.0% | |
| AATTTCCTGGAACCGGTGAG+AGG | + | chr4.4:29413740-29413759 | MS.gene56450:CDS | 50.0% | |
| ATTTCCTGGAACCGGTGAGA+GGG | + | chr4.4:29413741-29413760 | MS.gene56450:CDS | 50.0% | |
| ! | GAAGGTGATGATAACGAGGC+TGG | - | chr4.4:29413245-29413264 | None:intergenic | 50.0% |
| ! | GAGTAGCAGTCAAGTTGCTG+TGG | + | chr4.4:29413214-29413233 | MS.gene56450:CDS | 50.0% |
| !! | GCATCCCTCTCACCGGTTCC+AGG | - | chr4.4:29413748-29413767 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29413096 | 29413786 | 29413096 | ID=MS.gene56450 |
| chr4.4 | mRNA | 29413096 | 29413786 | 29413096 | ID=MS.gene56450.t1;Parent=MS.gene56450 |
| chr4.4 | exon | 29413096 | 29413123 | 29413096 | ID=MS.gene56450.t1.exon1;Parent=MS.gene56450.t1 |
| chr4.4 | CDS | 29413096 | 29413123 | 29413096 | ID=cds.MS.gene56450.t1;Parent=MS.gene56450.t1 |
| chr4.4 | exon | 29413213 | 29413361 | 29413213 | ID=MS.gene56450.t1.exon2;Parent=MS.gene56450.t1 |
| chr4.4 | CDS | 29413213 | 29413361 | 29413213 | ID=cds.MS.gene56450.t1;Parent=MS.gene56450.t1 |
| chr4.4 | exon | 29413475 | 29413786 | 29413475 | ID=MS.gene56450.t1.exon3;Parent=MS.gene56450.t1 |
| chr4.4 | CDS | 29413475 | 29413786 | 29413475 | ID=cds.MS.gene56450.t1;Parent=MS.gene56450.t1 |
| Gene Sequence |
| Protein sequence |
