Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56655.t1 | AES90030.2 | 86.5 | 156 | 18 | 1 | 10 | 162 | 38 | 193 | 5.40E-69 | 270.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56655.t1 | Q7XLP4 | 39.1 | 110 | 63 | 2 | 34 | 140 | 83 | 191 | 7.0e-17 | 88.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56655.t1 | G7JHL3 | 86.5 | 156 | 18 | 1 | 10 | 162 | 38 | 193 | 3.9e-69 | 270.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56655.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56655.t1 | MTR_4g083390 | 86.538 | 156 | 18 | 1 | 10 | 162 | 38 | 193 | 2.70e-95 | 274 |
| MS.gene56655.t1 | MTR_4g083390 | 90.977 | 133 | 12 | 0 | 30 | 162 | 1 | 133 | 3.90e-88 | 253 |
| MS.gene56655.t1 | MTR_4g083390 | 85.496 | 131 | 19 | 0 | 30 | 160 | 1 | 131 | 2.63e-80 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGAATTGAACCGCTTTC+AGG | 0.230161 | 4.4:+29425348 | MS.gene56655:CDS |
| TTCAAAGTTCAAATTCTTAG+AGG | 0.303256 | 4.4:+29425758 | MS.gene56655:CDS |
| AGTTGGGCCACGACTTATAA+TGG | 0.308961 | 4.4:+29425614 | MS.gene56655:CDS |
| ACGGGGACTAAGATGTGTTT+TGG | 0.312922 | 4.4:-29425397 | None:intergenic |
| TTCCGACTATTCTCAAATAT+CGG | 0.320685 | 4.4:+29425585 | MS.gene56655:CDS |
| GCATCCCTCTCACCGGTTCC+AGG | 0.356774 | 4.4:-29425812 | None:intergenic |
| TGAAATTACCAATCCATAAC+GGG | 0.384987 | 4.4:-29425415 | None:intergenic |
| GAGAATAGTCGGAACCGATT+TGG | 0.387243 | 4.4:-29425576 | None:intergenic |
| ACACATCTTAGTCCCCGTTA+TGG | 0.404304 | 4.4:+29425402 | MS.gene56655:CDS |
| GAAGATAATTATTTGAAGCT+TGG | 0.405476 | 4.4:+29425686 | MS.gene56655:CDS |
| TCCGACTATTCTCAAATATC+GGG | 0.408506 | 4.4:+29425586 | MS.gene56655:CDS |
| GCCAACGCTATCAAACAGTT+TGG | 0.414501 | 4.4:-29425648 | None:intergenic |
| AACTGTTTGATAGCGTTGGC+TGG | 0.415821 | 4.4:+29425651 | MS.gene56655:CDS |
| GAAAGGACCACATGAAAGTA+TGG | 0.437117 | 4.4:-29425375 | None:intergenic |
| CTTTACGAATTTCCTGGAAC+CGG | 0.450094 | 4.4:+29425800 | MS.gene56655:CDS |
| CAAATATCGGGGTAAGAGTT+GGG | 0.464634 | 4.4:+29425598 | MS.gene56655:CDS |
| GATTACTAATCTATGACAAA+TGG | 0.467072 | 4.4:-29425836 | None:intergenic |
| TCAAATATCGGGGTAAGAGT+TGG | 0.468409 | 4.4:+29425597 | MS.gene56655:CDS |
| TGAAGAACTTCCTTCCAAAT+CGG | 0.469161 | 4.4:+29425562 | MS.gene56655:CDS |
| GTTGGGCCACGACTTATAAT+GGG | 0.482324 | 4.4:+29425615 | MS.gene56655:CDS |
| ATGAAATTACCAATCCATAA+CGG | 0.499697 | 4.4:-29425416 | None:intergenic |
| ATGTCGTCTTCAAAATCAGC+TGG | 0.502794 | 4.4:+29425163 | MS.gene56655:CDS |
| ACCAAACTGTTTGATAGCGT+TGG | 0.508522 | 4.4:+29425647 | MS.gene56655:CDS |
| CCCCGATATTTGAGAATAGT+CGG | 0.515602 | 4.4:-29425587 | None:intergenic |
| TCTTAGTCCCCGTTATGGAT+TGG | 0.515821 | 4.4:+29425407 | MS.gene56655:CDS |
| ACCTTCGACAAATATGCAAA+CGG | 0.530039 | 4.4:+29425326 | MS.gene56655:CDS |
| ACTGTTTGATAGCGTTGGCT+GGG | 0.539366 | 4.4:+29425652 | MS.gene56655:CDS |
| GAAGGTGATGATAACGAGGC+TGG | 0.539673 | 4.4:-29425309 | None:intergenic |
| TCCGTTTGCATATTTGTCGA+AGG | 0.549182 | 4.4:-29425327 | None:intergenic |
| GTTCTGCCCATTATAAGTCG+TGG | 0.557426 | 4.4:-29425621 | None:intergenic |
| TCTTCAAAATCAGCTGGAAG+AGG | 0.564849 | 4.4:+29425169 | MS.gene56655:CDS |
| AAATGGTGCATCCCTCTCAC+CGG | 0.578086 | 4.4:-29425819 | None:intergenic |
| AGGAGTGCCATACTTTCATG+TGG | 0.578309 | 4.4:+29425368 | MS.gene56655:CDS |
| TCGACAAATATGCAAACGGA+TGG | 0.583245 | 4.4:+29425330 | MS.gene56655:CDS |
| GAGTAGCAGTCAAGTTGCTG+TGG | 0.583739 | 4.4:+29425281 | MS.gene56655:CDS |
| TCTTCACATATGCTGCTGCT+CGG | 0.585483 | 4.4:-29425545 | None:intergenic |
| ATTTCCTGGAACCGGTGAGA+GGG | 0.587927 | 4.4:+29425808 | MS.gene56655:CDS |
| AGTAGCAGTCAAGTTGCTGT+GGG | 0.603556 | 4.4:+29425282 | MS.gene56655:CDS |
| AATTTCCTGGAACCGGTGAG+AGG | 0.633829 | 4.4:+29425807 | MS.gene56655:CDS |
| ATAGTCGGAACCGATTTGGA+AGG | 0.643344 | 4.4:-29425572 | None:intergenic |
| AAGTATGGCACTCCTGAAAG+CGG | 0.678549 | 4.4:-29425360 | None:intergenic |
| CCGACTATTCTCAAATATCG+GGG | 0.681942 | 4.4:+29425587 | MS.gene56655:CDS |
| GAAATTACCAATCCATAACG+GGG | 0.716232 | 4.4:-29425414 | None:intergenic |
| TGTCGAAGGTGATGATAACG+AGG | 0.728967 | 4.4:-29425313 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATGTGATATGAAATACTA+AGG | - | chr4.4:29425202-29425221 | None:intergenic | 20.0% |
| !! | TTATAGATCAAACTAAATGA+AGG | + | chr4.4:29425456-29425475 | MS.gene56655:intron | 20.0% |
| ! | AAGATTAACTTTCATGTACA+CGG | + | chr4.4:29425224-29425243 | MS.gene56655:intron | 25.0% |
| ! | ATGAAATTACCAATCCATAA+CGG | - | chr4.4:29425419-29425438 | None:intergenic | 25.0% |
| ! | CTTGTATTGTATTGTATTGT+TGG | + | chr4.4:29425512-29425531 | MS.gene56655:intron | 25.0% |
| ! | TATTGTATTGTTGGCATATA+GGG | + | chr4.4:29425521-29425540 | MS.gene56655:intron | 25.0% |
| ! | TTCAAAGTTCAAATTCTTAG+AGG | + | chr4.4:29425758-29425777 | MS.gene56655:CDS | 25.0% |
| !! | ATCAAACTAAATGAAGGTTT+TGG | + | chr4.4:29425462-29425481 | MS.gene56655:intron | 25.0% |
| !! | GAAGATAATTATTTGAAGCT+TGG | + | chr4.4:29425686-29425705 | MS.gene56655:CDS | 25.0% |
| ATTGTATTGTTGGCATATAG+GGG | + | chr4.4:29425522-29425541 | MS.gene56655:intron | 30.0% | |
| GGAAATTCGTAAAGCAAAAA+TGG | - | chr4.4:29425794-29425813 | None:intergenic | 30.0% | |
| GTATTGTATTGTTGGCATAT+AGG | + | chr4.4:29425520-29425539 | MS.gene56655:intron | 30.0% | |
| GTGTACATGAAAGTTAATCT+TGG | - | chr4.4:29425225-29425244 | None:intergenic | 30.0% | |
| TGAAATTACCAATCCATAAC+GGG | - | chr4.4:29425418-29425437 | None:intergenic | 30.0% | |
| TTCCGACTATTCTCAAATAT+CGG | + | chr4.4:29425585-29425604 | MS.gene56655:CDS | 30.0% | |
| ! | TTTTTGCTTTACGAATTTCC+TGG | + | chr4.4:29425794-29425813 | MS.gene56655:CDS | 30.0% |
| !!! | ACTAAATGAAGGTTTTGGAA+AGG | + | chr4.4:29425467-29425486 | MS.gene56655:intron | 30.0% |
| ACCTTCGACAAATATGCAAA+CGG | + | chr4.4:29425326-29425345 | MS.gene56655:CDS | 35.0% | |
| GAAATTACCAATCCATAACG+GGG | - | chr4.4:29425417-29425436 | None:intergenic | 35.0% | |
| TCCGACTATTCTCAAATATC+GGG | + | chr4.4:29425586-29425605 | MS.gene56655:CDS | 35.0% | |
| TGAAGAACTTCCTTCCAAAT+CGG | + | chr4.4:29425562-29425581 | MS.gene56655:CDS | 35.0% | |
| TTAACTTTCATGTACACGGT+CGG | + | chr4.4:29425228-29425247 | MS.gene56655:intron | 35.0% | |
| !!! | GACTAAGATGTGTTTTGGAA+AGG | - | chr4.4:29425395-29425414 | None:intergenic | 35.0% |
| ATGTCGTCTTCAAAATCAGC+TGG | + | chr4.4:29425163-29425182 | MS.gene56655:CDS | 40.0% | |
| CAAATATCGGGGTAAGAGTT+GGG | + | chr4.4:29425598-29425617 | MS.gene56655:CDS | 40.0% | |
| CCCCGATATTTGAGAATAGT+CGG | - | chr4.4:29425590-29425609 | None:intergenic | 40.0% | |
| CCGACTATTCTCAAATATCG+GGG | + | chr4.4:29425587-29425606 | MS.gene56655:CDS | 40.0% | |
| CTTTACGAATTTCCTGGAAC+CGG | + | chr4.4:29425800-29425819 | MS.gene56655:CDS | 40.0% | |
| GAAAGGACCACATGAAAGTA+TGG | - | chr4.4:29425378-29425397 | None:intergenic | 40.0% | |
| GCTCATGAAAAACAGTGAAG+AGG | + | chr4.4:29425727-29425746 | MS.gene56655:CDS | 40.0% | |
| TCAAATATCGGGGTAAGAGT+TGG | + | chr4.4:29425597-29425616 | MS.gene56655:CDS | 40.0% | |
| TCCGTTTGCATATTTGTCGA+AGG | - | chr4.4:29425330-29425349 | None:intergenic | 40.0% | |
| TCGACAAATATGCAAACGGA+TGG | + | chr4.4:29425330-29425349 | MS.gene56655:CDS | 40.0% | |
| TCTTCAAAATCAGCTGGAAG+AGG | + | chr4.4:29425169-29425188 | MS.gene56655:CDS | 40.0% | |
| !! | ACCAAACTGTTTGATAGCGT+TGG | + | chr4.4:29425647-29425666 | MS.gene56655:CDS | 40.0% |
| AAGTATGGCACTCCTGAAAG+CGG | - | chr4.4:29425363-29425382 | None:intergenic | 45.0% | |
| ACACATCTTAGTCCCCGTTA+TGG | + | chr4.4:29425402-29425421 | MS.gene56655:CDS | 45.0% | |
| AGTTGGGCCACGACTTATAA+TGG | + | chr4.4:29425614-29425633 | MS.gene56655:CDS | 45.0% | |
| GAGAATAGTCGGAACCGATT+TGG | - | chr4.4:29425579-29425598 | None:intergenic | 45.0% | |
| GATGGAATTGAACCGCTTTC+AGG | + | chr4.4:29425348-29425367 | MS.gene56655:CDS | 45.0% | |
| GCCAACGCTATCAAACAGTT+TGG | - | chr4.4:29425651-29425670 | None:intergenic | 45.0% | |
| GTTCTGCCCATTATAAGTCG+TGG | - | chr4.4:29425624-29425643 | None:intergenic | 45.0% | |
| GTTGGGCCACGACTTATAAT+GGG | + | chr4.4:29425615-29425634 | MS.gene56655:CDS | 45.0% | |
| TCTTAGTCCCCGTTATGGAT+TGG | + | chr4.4:29425407-29425426 | MS.gene56655:CDS | 45.0% | |
| TCTTCACATATGCTGCTGCT+CGG | - | chr4.4:29425548-29425567 | None:intergenic | 45.0% | |
| ! | ACGGGGACTAAGATGTGTTT+TGG | - | chr4.4:29425400-29425419 | None:intergenic | 45.0% |
| ! | AGTAGCAGTCAAGTTGCTGT+GGG | + | chr4.4:29425282-29425301 | MS.gene56655:CDS | 45.0% |
| ! | TGTCGAAGGTGATGATAACG+AGG | - | chr4.4:29425316-29425335 | None:intergenic | 45.0% |
| !! | AACTGTTTGATAGCGTTGGC+TGG | + | chr4.4:29425651-29425670 | MS.gene56655:CDS | 45.0% |
| !! | ACTGTTTGATAGCGTTGGCT+GGG | + | chr4.4:29425652-29425671 | MS.gene56655:CDS | 45.0% |
| !! | AGGAGTGCCATACTTTCATG+TGG | + | chr4.4:29425368-29425387 | MS.gene56655:CDS | 45.0% |
| !! | ATAGTCGGAACCGATTTGGA+AGG | - | chr4.4:29425575-29425594 | None:intergenic | 45.0% |
| AAATGGTGCATCCCTCTCAC+CGG | - | chr4.4:29425822-29425841 | None:intergenic | 50.0% | |
| AATTTCCTGGAACCGGTGAG+AGG | + | chr4.4:29425807-29425826 | MS.gene56655:CDS | 50.0% | |
| ATTTCCTGGAACCGGTGAGA+GGG | + | chr4.4:29425808-29425827 | MS.gene56655:CDS | 50.0% | |
| ! | GAAGGTGATGATAACGAGGC+TGG | - | chr4.4:29425312-29425331 | None:intergenic | 50.0% |
| ! | GAGTAGCAGTCAAGTTGCTG+TGG | + | chr4.4:29425281-29425300 | MS.gene56655:CDS | 50.0% |
| !! | GCATCCCTCTCACCGGTTCC+AGG | - | chr4.4:29425815-29425834 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 29425163 | 29425853 | 29425163 | ID=MS.gene56655 |
| chr4.4 | mRNA | 29425163 | 29425853 | 29425163 | ID=MS.gene56655.t1;Parent=MS.gene56655 |
| chr4.4 | exon | 29425163 | 29425190 | 29425163 | ID=MS.gene56655.t1.exon1;Parent=MS.gene56655.t1 |
| chr4.4 | CDS | 29425163 | 29425190 | 29425163 | ID=cds.MS.gene56655.t1;Parent=MS.gene56655.t1 |
| chr4.4 | exon | 29425280 | 29425428 | 29425280 | ID=MS.gene56655.t1.exon2;Parent=MS.gene56655.t1 |
| chr4.4 | CDS | 29425280 | 29425428 | 29425280 | ID=cds.MS.gene56655.t1;Parent=MS.gene56655.t1 |
| chr4.4 | exon | 29425542 | 29425853 | 29425542 | ID=MS.gene56655.t1.exon3;Parent=MS.gene56655.t1 |
| chr4.4 | CDS | 29425542 | 29425853 | 29425542 | ID=cds.MS.gene56655.t1;Parent=MS.gene56655.t1 |
| Gene Sequence |
| Protein sequence |
