AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56686.t1 MTR_5g081860 98.893 271 3 0 1 271 1 271 0.0 564
MS.gene56686.t1 MTR_7g067080 62.150 214 72 2 3 209 11 222 9.46e-90 269
MS.gene56686.t1 MTR_5g069710 61.386 202 71 3 2 198 4 203 9.85e-85 256
MS.gene56686.t1 MTR_3g462790 64.796 196 56 4 16 200 27 220 2.21e-83 253
MS.gene56686.t1 MTR_1g111830 49.733 187 80 4 25 204 22 201 4.75e-55 178
MS.gene56686.t1 MTR_8g063600 48.634 183 92 1 20 202 3 183 1.33e-50 168
MS.gene56686.t1 MTR_4g111975 42.381 210 81 5 27 201 8 212 3.15e-47 159
MS.gene56686.t1 MTR_8g063870 46.111 180 79 3 25 202 6 169 3.71e-42 145
MS.gene56686.t1 MTR_2g100930 61.111 90 33 1 120 209 104 191 1.67e-29 114
MS.gene56686.t1 MTR_6g092540 69.863 73 20 1 119 191 91 161 8.70e-29 111
MS.gene56686.t1 MTR_4g100630 64.198 81 27 1 120 200 90 168 7.86e-28 109
MS.gene56686.t1 MTR_4g015130 71.429 70 18 1 134 203 2 69 1.05e-27 102
MS.gene56686.t1 MTR_8g101650 60.714 84 30 1 116 199 96 176 7.56e-27 105
MS.gene56686.t1 MTR_5g037080 60.000 90 34 1 112 201 89 176 8.38e-26 103
MS.gene56686.t1 MTR_4g107230 71.186 59 17 0 113 171 72 130 1.31e-24 100
MS.gene56686.t1 MTR_5g027570 49.524 105 49 1 92 192 55 159 1.02e-23 96.3
MS.gene56686.t1 MTR_5g088010 58.904 73 29 1 121 192 56 128 5.04e-23 95.1
MS.gene56686.t1 MTR_1g033600 39.695 131 65 3 25 142 10 139 6.06e-23 91.7
MS.gene56686.t1 MTR_3g104370 51.429 105 44 4 90 191 58 158 1.08e-22 93.6
MS.gene56686.t1 MTR_5g088060 58.025 81 32 2 113 192 261 340 1.08e-21 94.4
MS.gene56686.t1 MTR_5g488160 58.025 81 32 2 113 192 261 340 1.08e-21 94.4
MS.gene56686.t1 MTR_1g033620 70.968 62 16 1 141 202 21 80 2.57e-21 90.1
MS.gene56686.t1 MTR_1g022290 55.882 68 30 0 22 89 2 69 5.23e-21 85.1
MS.gene56686.t1 MTR_0036s0260 57.971 69 27 1 125 193 28 94 3.11e-20 86.7
MS.gene56686.t1 MTR_2g090305 57.971 69 27 1 125 193 28 94 8.88e-20 84.7
MS.gene56686.t1 MTR_3g111880 53.846 65 30 0 25 89 5 69 1.92e-19 81.3
MS.gene56686.t1 MTR_6g004250 52.941 68 32 0 22 89 5 72 1.08e-18 78.6
MS.gene56686.t1 MTR_5g027550 58.904 73 29 1 121 192 73 145 1.50e-18 82.4
MS.gene56686.t1 MTR_5g075760 33.000 200 119 7 26 218 12 203 2.72e-18 81.6
MS.gene56686.t1 MTR_3g070110 40.764 157 79 7 48 196 21 171 3.54e-18 81.3
MS.gene56686.t1 MTR_7g089210 52.941 68 32 0 30 97 15 82 5.62e-18 77.0
MS.gene56686.t1 MTR_5g020170 45.333 75 41 0 15 89 7 81 1.69e-17 76.3
MS.gene56686.t1 MTR_3g116720 52.381 63 30 0 27 89 16 78 2.00e-17 75.5
MS.gene56686.t1 MTR_3g013440 47.945 73 37 1 18 89 1 73 1.28e-16 73.6
MS.gene56686.t1 MTR_5g075790 30.000 200 124 5 26 218 21 211 1.40e-16 77.0
MS.gene56686.t1 MTR_1g048660 53.030 66 29 1 126 191 13 76 1.67e-16 73.2
MS.gene56686.t1 MTR_8g077360 49.231 65 32 1 25 89 3 66 2.80e-15 69.3
MS.gene56686.t1 MTR_8g077380 49.231 65 32 1 25 89 3 66 2.93e-15 69.3
MS.gene56686.t1 MTR_8g077390 49.231 65 32 1 25 89 3 66 2.93e-15 69.3
MS.gene56686.t1 MTR_8g077420 50.820 61 29 1 27 87 5 64 6.15e-14 65.9
MS.gene56686.t1 MTR_5g020540 50.820 61 29 1 27 87 4 63 3.14e-13 63.5
MS.gene56686.t1 MTR_1g083180 55.714 70 29 2 127 195 63 131 3.37e-13 67.0
MS.gene56686.t1 MTR_3g111920 41.270 63 37 0 27 89 3 65 9.51e-13 62.8
MS.gene56686.t1 MTR_6g043490 41.026 78 41 2 125 197 107 184 5.93e-12 64.3
MS.gene56686.t1 MTR_6g043480 53.191 47 22 0 125 171 107 153 6.41e-12 63.9
MS.gene56686.t1 MTR_8g072990 50.000 50 25 0 122 171 97 146 9.91e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56686.t1 AT2G38090 59.716 211 69 2 3 199 5 213 4.20e-86 259
MS.gene56686.t1 AT3G11280 59.910 222 70 6 3 212 1 215 1.18e-80 244
MS.gene56686.t1 AT3G11280 59.910 222 70 6 3 212 1 215 1.18e-80 244
MS.gene56686.t1 AT5G05790 56.828 227 88 6 3 222 4 227 1.41e-79 241
MS.gene56686.t1 AT5G05790 56.828 227 88 6 3 222 4 227 1.41e-79 241
MS.gene56686.t1 AT5G58900 58.019 212 70 4 16 210 15 224 4.01e-77 236
MS.gene56686.t1 AT5G01200 54.067 209 73 6 6 196 12 215 4.05e-67 209
MS.gene56686.t1 AT5G08520 51.087 184 80 2 27 202 12 193 9.10e-54 176
MS.gene56686.t1 AT1G49010 45.050 202 81 4 21 194 2 201 1.76e-49 166
MS.gene56686.t1 AT5G04760 43.333 210 89 7 25 231 4 186 2.94e-46 154
MS.gene56686.t1 AT5G23650 42.654 211 89 5 20 215 6 199 5.51e-44 152
MS.gene56686.t1 AT3G10580 38.164 207 98 7 27 231 8 186 2.20e-35 127
MS.gene56686.t1 AT3G10580 38.164 207 98 7 27 231 8 186 4.51e-35 127
MS.gene56686.t1 AT4G09450 42.515 167 80 4 26 192 6 156 4.31e-32 117
MS.gene56686.t1 AT5G56840 63.415 82 29 1 120 201 86 166 1.11e-27 107
MS.gene56686.t1 AT5G47390 66.250 80 25 1 112 191 83 160 1.26e-27 109
MS.gene56686.t1 AT5G61620 52.941 102 46 1 91 192 74 173 7.05e-27 106
MS.gene56686.t1 AT1G19000 51.327 113 48 3 112 219 89 199 3.36e-25 101
MS.gene56686.t1 AT1G19000 51.327 113 48 3 112 219 89 199 3.36e-25 101
MS.gene56686.t1 AT1G70000 72.131 61 17 0 112 172 85 145 6.02e-25 100
MS.gene56686.t1 AT1G70000 72.131 61 17 0 112 172 85 145 6.02e-25 100
MS.gene56686.t1 AT3G16350 55.238 105 42 2 116 217 125 227 6.11e-25 102
MS.gene56686.t1 AT3G10590 40.196 204 96 12 27 216 7 198 5.08e-23 94.0
MS.gene56686.t1 AT1G74840 75.000 52 13 0 120 171 91 142 5.57e-22 92.0
MS.gene56686.t1 AT1G74840 75.000 52 13 0 120 171 91 142 7.96e-22 92.0
MS.gene56686.t1 AT1G75250 55.556 63 28 0 27 89 12 74 6.82e-18 77.0
MS.gene56686.t1 AT1G75250 55.556 63 28 0 27 89 12 74 6.95e-18 78.2
MS.gene56686.t1 AT4G39250 48.485 66 34 0 27 92 14 79 1.56e-17 76.3
MS.gene56686.t1 AT2G21650 47.761 67 35 0 27 93 14 80 3.82e-16 72.8
MS.gene56686.t1 AT2G18328 54.098 61 27 1 29 89 14 73 4.12e-14 66.6
MS.gene56686.t1 AT1G19510 51.724 58 28 0 32 89 17 74 4.71e-13 64.3
MS.gene56686.t1 AT3G10595 29.054 148 81 5 24 171 3 126 2.62e-12 64.3

Find 52 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCCTATGCAGCAGAAAA+TGG 0.222210 5.1:+63940970 MS.gene56686:CDS
TATGCTAAGCTGAAAGTTTC+GGG 0.251091 5.1:+63941067 MS.gene56686:CDS
ACCAGGAATCGGAACATTTC+CGG 0.268577 5.1:-63939864 None:intergenic
GCCGGAAATGTTCCGATTCC+TGG 0.317971 5.1:+63939863 MS.gene56686:CDS
TTATGCTAAGCTGAAAGTTT+CGG 0.326332 5.1:+63941066 MS.gene56686:CDS
CTTCTCTACTCCATTCTGTT+TGG 0.332735 5.1:-63939691 None:intergenic
AGGAGACACATTGACTAATA+AGG 0.335368 5.1:-63940954 None:intergenic
ACATAAAATAAGGAGAATAA+AGG 0.337256 5.1:-63939637 None:intergenic
TGTGATTTAAGGTATGGATT+TGG 0.345223 5.1:+63939610 None:intergenic
TGATGTGATTAAGCAGTATA+GGG 0.364968 5.1:+63939811 MS.gene56686:CDS
AGAAGGCATGTGACTGTTAG+AGG 0.384719 5.1:+63939947 MS.gene56686:CDS
AAGATTGCTGAGATGATTCC+TGG 0.391655 5.1:+63939776 MS.gene56686:CDS
TTCGTATCCAACATAAAATA+AGG 0.394704 5.1:-63939647 None:intergenic
TTGATGTGATTAAGCAGTAT+AGG 0.399334 5.1:+63939810 MS.gene56686:CDS
ATAAGGCAAAAGGTTTCTTC+TGG 0.415949 5.1:+63940848 MS.gene56686:CDS
GCAATCTTAAGCCATCTATC+TGG 0.417904 5.1:-63939761 None:intergenic
TAAAGTATGGCAAAGGCGAC+TGG 0.445487 5.1:+63940753 MS.gene56686:CDS
CATAAAATAAGGAGAATAAA+GGG 0.445828 5.1:-63939636 None:intergenic
GATTAAGCAGTATAGGGAAT+TGG 0.449715 5.1:+63939817 MS.gene56686:CDS
GCATGACTTGCTACTTGTGT+TGG 0.455619 5.1:-63940812 None:intergenic
TGCTCTTGCTATATATGATA+AGG 0.457525 5.1:+63939733 MS.gene56686:CDS
ATAAGGATACACCAGATAGA+TGG 0.475384 5.1:+63939750 MS.gene56686:CDS
ATGCACAAAAGTACTACATA+AGG 0.480834 5.1:+63940831 MS.gene56686:CDS
AGAGGAAGAAAGGTGTTCCT+TGG 0.489408 5.1:+63939981 MS.gene56686:CDS
AAAGTACTACATAAGGCAAA+AGG 0.510073 5.1:+63940838 MS.gene56686:CDS
AATAAGGACTTGTTATCTGA+TGG 0.512003 5.1:-63940938 None:intergenic
GAGGCAAGATAACCAGGAAT+CGG 0.520347 5.1:-63939875 None:intergenic
TGATGGATCACCACAAGGGC+AGG 0.524353 5.1:+63941015 MS.gene56686:CDS
TCAGCTTAGCATAAGCCTCA+TGG 0.535139 5.1:-63941057 None:intergenic
TCTCCTATGCAGCAGAAAAT+GGG 0.535185 5.1:+63940971 MS.gene56686:CDS
AGTACTAGCCACTACAATGA+TGG 0.540673 5.1:+63940998 MS.gene56686:CDS
TCTGATCATGAGAGGAAGAA+AGG 0.544690 5.1:+63939971 MS.gene56686:CDS
AAAGAAGAGGCAAGATAACC+AGG 0.552310 5.1:-63939881 None:intergenic
AAGAGAGTTACCAAACAGAA+TGG 0.556624 5.1:+63939681 MS.gene56686:CDS
AGGCAAAAGGTTTCTTCTGG+AGG 0.582472 5.1:+63940851 MS.gene56686:CDS
ACTATGATGGAAACAGAAGA+AGG 0.582942 5.1:+63939930 MS.gene56686:CDS
TCATAGAGATCCTGCCCTTG+TGG 0.585420 5.1:-63941025 None:intergenic
CTTGGACTGAAGAGGAACAT+AGG 0.585629 5.1:+63939999 MS.gene56686:CDS
CTATGTTCCTCTTCAGTCCA+AGG 0.586053 5.1:-63939998 None:intergenic
CTATGCAGCAGAAAATGGGT+TGG 0.587490 5.1:+63940975 MS.gene56686:CDS
AGGTGTTCCTTGGACTGAAG+AGG 0.592742 5.1:+63939991 MS.gene56686:CDS
GTTGTGATATCATGAATGCT+TGG 0.600782 5.1:-63940890 None:intergenic
CTATGACTGCTCTTTCCATG+AGG 0.601527 5.1:+63941042 MS.gene56686:CDS
GCTTCTAGAGACTTCAACAA+AGG 0.612158 5.1:+63941100 MS.gene56686:CDS
GATGTGAGTGAAATTGAGGC+CGG 0.613302 5.1:+63939845 MS.gene56686:CDS
TACAATGATGGATCACCACA+AGG 0.622159 5.1:+63941010 MS.gene56686:CDS
AACTTCAAAGGTGAAAGAAG+AGG 0.626616 5.1:-63939894 None:intergenic
ACATCAATAACAGTCTTACC+AGG 0.630015 5.1:-63939794 None:intergenic
TTAGAGGTTCTGATCATGAG+AGG 0.633143 5.1:+63939963 MS.gene56686:CDS
GTGGTGATCCATCATTGTAG+TGG 0.633599 5.1:-63941006 None:intergenic
AGAAGATGTGAGTGAAATTG+AGG 0.635385 5.1:+63939841 MS.gene56686:CDS
ACAATGATGGATCACCACAA+GGG 0.720384 5.1:+63941011 MS.gene56686:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAACCTAAAATCT+AGG - chr5.1:63940368-63940387 None:intergenic 15.0%
!!! AATAACAAGTAACTTTTAAA+AGG - chr5.1:63940543-63940562 None:intergenic 15.0%
!!! ATAACAAGTAACTTTTAAAA+GGG - chr5.1:63940542-63940561 None:intergenic 15.0%
!!! ATAACAAGTAACTTTTATAA+CGG - chr5.1:63940672-63940691 None:intergenic 15.0%
!! ACATAAAATAAGGAGAATAA+AGG - chr5.1:63939640-63939659 None:intergenic 20.0%
!! AGTAAAAGAACAAAAAGAAA+AGG - chr5.1:63940056-63940075 None:intergenic 20.0%
!! CATAAAATAAGGAGAATAAA+GGG - chr5.1:63939639-63939658 None:intergenic 20.0%
!!! ATTTCATTTTCCTAGATTTT+AGG + chr5.1:63940355-63940374 MS.gene56686:intron 20.0%
!!! TAACAAGTAACTTTTAAAAG+GGG - chr5.1:63940541-63940560 None:intergenic 20.0%
!!! TAACAAGTAACTTTTATAAC+GGG - chr5.1:63940671-63940690 None:intergenic 20.0%
!!! TTATTCTCCTTATTTTATGT+TGG + chr5.1:63939640-63939659 MS.gene56686:CDS 20.0%
!!! TTGTTATTGAACTTGATTAT+TGG + chr5.1:63940555-63940574 MS.gene56686:intron 20.0%
! AGAAAAATTGAAGTAAGACA+CGG + chr5.1:63940136-63940155 MS.gene56686:intron 25.0%
! AGAGACGGAAAAAATAAAAT+CGG + chr5.1:63940164-63940183 MS.gene56686:intron 25.0%
! TAGCTAATGAATCATATTCA+TGG + chr5.1:63940304-63940323 MS.gene56686:intron 25.0%
! TTCGTATCCAACATAAAATA+AGG - chr5.1:63939650-63939669 None:intergenic 25.0%
!! AACAAGTAACTTTTATAACG+GGG - chr5.1:63940670-63940689 None:intergenic 25.0%
!! ATTGAACTTGATTATTGGTT+TGG + chr5.1:63940560-63940579 MS.gene56686:intron 25.0%
!! ATTTTATGTTGGATACGAAT+TGG + chr5.1:63939651-63939670 MS.gene56686:CDS 25.0%
!!! ATGTTAAATTGAGTTGGTTT+AGG + chr5.1:63940632-63940651 MS.gene56686:intron 25.0%
!!! TTTTTCTAGTAGTATACCAT+AGG - chr5.1:63940123-63940142 None:intergenic 25.0%
ATCGACATGTTAAATTGAGT+TGG + chr5.1:63940626-63940645 MS.gene56686:intron 30.0%
ATTTACTCTTTGTGCATGTT+TGG + chr5.1:63940695-63940714 MS.gene56686:intron 30.0%
GAAAAAACTTCCATAGAGAA+AGG - chr5.1:63940079-63940098 None:intergenic 30.0%
GTCGAAAAACAAAACTATGA+TGG + chr5.1:63939917-63939936 MS.gene56686:CDS 30.0%
TGATGTGATTAAGCAGTATA+GGG + chr5.1:63939811-63939830 MS.gene56686:CDS 30.0%
TTATGCTAAGCTGAAAGTTT+CGG + chr5.1:63941066-63941085 MS.gene56686:CDS 30.0%
TTGATGTGATTAAGCAGTAT+AGG + chr5.1:63939810-63939829 MS.gene56686:CDS 30.0%
! AAAGTACTACATAAGGCAAA+AGG + chr5.1:63940838-63940857 MS.gene56686:CDS 30.0%
! TGCTCTTGCTATATATGATA+AGG + chr5.1:63939733-63939752 MS.gene56686:CDS 30.0%
! TTCTTTTACTCCTTTCTCTA+TGG + chr5.1:63940066-63940085 MS.gene56686:intron 30.0%
!! AATAAGGACTTGTTATCTGA+TGG - chr5.1:63940941-63940960 None:intergenic 30.0%
!! ATGCACAAAAGTACTACATA+AGG + chr5.1:63940831-63940850 MS.gene56686:CDS 30.0%
!! GTAACTTTTAAAAGGGGTAA+AGG - chr5.1:63940535-63940554 None:intergenic 30.0%
!! TAACTTTTATAACGGGGTAA+AGG - chr5.1:63940664-63940683 None:intergenic 30.0%
!! TTGTTTTTCGACAACTTCAA+AGG - chr5.1:63939909-63939928 None:intergenic 30.0%
!!! GGAAGTTTTTTCTTAGATTG+TGG + chr5.1:63940087-63940106 MS.gene56686:intron 30.0%
AACTTCAAAGGTGAAAGAAG+AGG - chr5.1:63939897-63939916 None:intergenic 35.0%
AAGAGAGTTACCAAACAGAA+TGG + chr5.1:63939681-63939700 MS.gene56686:CDS 35.0%
ACATCAATAACAGTCTTACC+AGG - chr5.1:63939797-63939816 None:intergenic 35.0%
AGAAGATGTGAGTGAAATTG+AGG + chr5.1:63939841-63939860 MS.gene56686:CDS 35.0%
ATAAGGATACACCAGATAGA+TGG + chr5.1:63939750-63939769 MS.gene56686:CDS 35.0%
ATAAGGCAAAAGGTTTCTTC+TGG + chr5.1:63940848-63940867 MS.gene56686:CDS 35.0%
GAGTCTGTAACACTTAATGA+TGG + chr5.1:63940198-63940217 MS.gene56686:intron 35.0%
GATTAAGCAGTATAGGGAAT+TGG + chr5.1:63939817-63939836 MS.gene56686:CDS 35.0%
GTTGTGATATCATGAATGCT+TGG - chr5.1:63940893-63940912 None:intergenic 35.0%
TATGCTAAGCTGAAAGTTTC+GGG + chr5.1:63941067-63941086 MS.gene56686:CDS 35.0%
TTGTGGAGAAAGAAAACCTA+TGG + chr5.1:63940104-63940123 MS.gene56686:intron 35.0%
! ACTATGATGGAAACAGAAGA+AGG + chr5.1:63939930-63939949 MS.gene56686:CDS 35.0%
! AGGAGACACATTGACTAATA+AGG - chr5.1:63940957-63940976 None:intergenic 35.0%
! TTTGTGCATGTTTGGAATCA+CGG + chr5.1:63940703-63940722 MS.gene56686:intron 35.0%
!! TTCAACAAAGGAGCTGTTTT+TGG + chr5.1:63941112-63941131 MS.gene56686:CDS 35.0%
AAAGAAGAGGCAAGATAACC+AGG - chr5.1:63939884-63939903 None:intergenic 40.0%
AAGATTGCTGAGATGATTCC+TGG + chr5.1:63939776-63939795 MS.gene56686:CDS 40.0%
AGTACTAGCCACTACAATGA+TGG + chr5.1:63940998-63941017 MS.gene56686:CDS 40.0%
CTTCTCTACTCCATTCTGTT+TGG - chr5.1:63939694-63939713 None:intergenic 40.0%
GCAATCTTAAGCCATCTATC+TGG - chr5.1:63939764-63939783 None:intergenic 40.0%
GTAAAGGCAAACTCCAATTG+AGG - chr5.1:63940519-63940538 None:intergenic 40.0%
TCTCCTATGCAGCAGAAAAT+GGG + chr5.1:63940971-63940990 MS.gene56686:CDS 40.0%
TCTGATCATGAGAGGAAGAA+AGG + chr5.1:63939971-63939990 MS.gene56686:CDS 40.0%
! GCTTCTAGAGACTTCAACAA+AGG + chr5.1:63941100-63941119 MS.gene56686:CDS 40.0%
! TTAGAGGTTCTGATCATGAG+AGG + chr5.1:63939963-63939982 MS.gene56686:CDS 40.0%
!! ACAATGATGGATCACCACAA+GGG + chr5.1:63941011-63941030 MS.gene56686:CDS 40.0%
!! TACAATGATGGATCACCACA+AGG + chr5.1:63941010-63941029 MS.gene56686:CDS 40.0%
!!! CTGATGGGGCTTTTAAAGTA+TGG + chr5.1:63940740-63940759 MS.gene56686:CDS 40.0%
!!! GGGCTTTTAAAGTATGGCAA+AGG + chr5.1:63940746-63940765 MS.gene56686:CDS 40.0%
ACCAGGAATCGGAACATTTC+CGG - chr5.1:63939867-63939886 None:intergenic 45.0%
AGGCAAAAGGTTTCTTCTGG+AGG + chr5.1:63940851-63940870 MS.gene56686:CDS 45.0%
CTATGACTGCTCTTTCCATG+AGG + chr5.1:63941042-63941061 MS.gene56686:CDS 45.0%
CTATGCAGCAGAAAATGGGT+TGG + chr5.1:63940975-63940994 MS.gene56686:CDS 45.0%
CTATGTTCCTCTTCAGTCCA+AGG - chr5.1:63940001-63940020 None:intergenic 45.0%
CTTGGACTGAAGAGGAACAT+AGG + chr5.1:63939999-63940018 MS.gene56686:CDS 45.0%
GAGGCAAGATAACCAGGAAT+CGG - chr5.1:63939878-63939897 None:intergenic 45.0%
GATGTGAGTGAAATTGAGGC+CGG + chr5.1:63939845-63939864 MS.gene56686:CDS 45.0%
GCAGATAATGCAGCCTCAAT+TGG + chr5.1:63940503-63940522 MS.gene56686:intron 45.0%
GTCTCCTATGCAGCAGAAAA+TGG + chr5.1:63940970-63940989 MS.gene56686:CDS 45.0%
GTGGTGATCCATCATTGTAG+TGG - chr5.1:63941009-63941028 None:intergenic 45.0%
TAAAGTATGGCAAAGGCGAC+TGG + chr5.1:63940753-63940772 MS.gene56686:CDS 45.0%
TAAGACACGGAAGCTAGAGA+CGG + chr5.1:63940149-63940168 MS.gene56686:intron 45.0%
TCAGCTTAGCATAAGCCTCA+TGG - chr5.1:63941060-63941079 None:intergenic 45.0%
! AGAAGGCATGTGACTGTTAG+AGG + chr5.1:63939947-63939966 MS.gene56686:CDS 45.0%
! CAACCCATTTTCTGCTGCAT+AGG - chr5.1:63940977-63940996 None:intergenic 45.0%
! CGTACAGACGTTTTCTGATG+GGG + chr5.1:63940726-63940745 MS.gene56686:intron 45.0%
! GCGTACAGACGTTTTCTGAT+GGG + chr5.1:63940725-63940744 MS.gene56686:intron 45.0%
!! AGAGGAAGAAAGGTGTTCCT+TGG + chr5.1:63939981-63940000 MS.gene56686:CDS 45.0%
!! GCATGACTTGCTACTTGTGT+TGG - chr5.1:63940815-63940834 None:intergenic 45.0%
AGGTGTTCCTTGGACTGAAG+AGG + chr5.1:63939991-63940010 MS.gene56686:CDS 50.0%
TCATAGAGATCCTGCCCTTG+TGG - chr5.1:63941028-63941047 None:intergenic 50.0%
! GGCGTACAGACGTTTTCTGA+TGG + chr5.1:63940724-63940743 MS.gene56686:intron 50.0%
GCCGGAAATGTTCCGATTCC+TGG + chr5.1:63939863-63939882 MS.gene56686:CDS 55.0%
! TGATGGATCACCACAAGGGC+AGG + chr5.1:63941015-63941034 MS.gene56686:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 63939623 63941150 63939623 ID=MS.gene56686
chr5.1 mRNA 63939623 63941150 63939623 ID=MS.gene56686.t1;Parent=MS.gene56686
chr5.1 exon 63939623 63940020 63939623 ID=MS.gene56686.t1.exon1;Parent=MS.gene56686.t1
chr5.1 CDS 63939623 63940020 63939623 ID=cds.MS.gene56686.t1;Parent=MS.gene56686.t1
chr5.1 exon 63940733 63941150 63940733 ID=MS.gene56686.t1.exon2;Parent=MS.gene56686.t1
chr5.1 CDS 63940733 63941150 63940733 ID=cds.MS.gene56686.t1;Parent=MS.gene56686.t1
Gene Sequence

>MS.gene56686

ATGGATTTGGAAACCCTTTATTCTCCTTATTTTATGTTGGATACGAATTGGCTTGTTGAAGAGAGTTACCAAACAGAATGGAGTAGAGAAGATAACAAGAAATTTGAAAGTGCTCTTGCTATATATGATAAGGATACACCAGATAGATGGCTTAAGATTGCTGAGATGATTCCTGGTAAGACTGTTATTGATGTGATTAAGCAGTATAGGGAATTGGTAGAAGATGTGAGTGAAATTGAGGCCGGAAATGTTCCGATTCCTGGTTATCTTGCCTCTTCTTTCACCTTTGAAGTTGTCGAAAAACAAAACTATGATGGAAACAGAAGAAGGCATGTGACTGTTAGAGGTTCTGATCATGAGAGGAAGAAAGGTGTTCCTTGGACTGAAGAGGAACATAGGTGAGCTTCATCAATCTAGAATCTTCAATTGTCCTTTTCTTTTTGTTCTTTTACTCCTTTCTCTATGGAAGTTTTTTCTTAGATTGTGGAGAAAGAAAACCTATGGTATACTACTAGAAAAATTGAAGTAAGACACGGAAGCTAGAGACGGAAAAAATAAAATCGGTCACTAAATTTGAGTCTGTAACACTTAATGATGGAGTTTGAGACGAATTTCTCATTTTTTTCGTCTCAAGATTTCTGTCGCTATTTCAATTTTTCAAGTAGTATTGATATTGAAATTTAGCTAATGAATCATATTCATGGATATACTGCAATAGCAACTCAATTTTTCATTTCATTTTCCTAGATTTTAGGTTTTTTATTTCTCATACAATTCTAATCGACTTTTATAATAGCTGATTTCTTAATATAGTGAAATAATTTGATTTTAGTAGCAGTCATGTTGCGATCTTTGATATTGCGAAAAATTACAATCACATGCAGATAATGCAGCCTCAATTGGAGTTTGCCTTTACCCCTTTTAAAAGTTACTTGTTATTGAACTTGATTATTGGTTTGGATAAGTATATTAAATTTTGAACTTCATGCAAAAGCACAAATCAATCGACATGTTAAATTGAGTTGGTTTAGGAGTTTGCCTTTACCCCGTTATAAAAGTTACTTGTTATTGAATTTACTCTTTGTGCATGTTTGGAATCACGGCGTACAGACGTTTTCTGATGGGGCTTTTAAAGTATGGCAAAGGCGACTGGAGAAACATCTCGCGCAATTTCGTCGTGACAAAGACTCCAACACAAGTAGCAAGTCATGCACAAAAGTACTACATAAGGCAAAAGGTTTCTTCTGGAGGCAAAGATAAAAGAAGACCAAGCATTCATGATATCACAACTGTTACTCTCACAGAAACTTCTTCTCCATCAGATAACAAGTCCTTATTAGTCAATGTGTCTCCTATGCAGCAGAAAATGGGTTGGAGTACTAGCCACTACAATGATGGATCACCACAAGGGCAGGATCTCTATGACTGCTCTTTCCATGAGGCTTATGCTAAGCTGAAAGTTTCGGGTTTCGCGACAGCTTCTAGAGACTTCAACAAAGGAGCTGTTTTTGGTATTCATGCACTGTAA
Protein sequence

>MS.gene56686.t1

MDLETLYSPYFMLDTNWLVEESYQTEWSREDNKKFESALAIYDKDTPDRWLKIAEMIPGKTVIDVIKQYRELVEDVSEIEAGNVPIPGYLASSFTFEVVEKQNYDGNRRRHVTVRGSDHERKKGVPWTEEEHRRFLMGLLKYGKGDWRNISRNFVVTKTPTQVASHAQKYYIRQKVSSGGKDKRRPSIHDITTVTLTETSSPSDNKSLLVNVSPMQQKMGWSTSHYNDGSPQGQDLYDCSFHEAYAKLKVSGFATASRDFNKGAVFGIHAL