Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56901.t1 | XP_013457766.1 | 81.7 | 361 | 21 | 2 | 1 | 316 | 1 | 361 | 1.10E-161 | 579.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56901.t1 | Q8LPH6 | 50.3 | 163 | 39 | 1 | 1 | 121 | 1 | 163 | 1.6e-36 | 154.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56901.t1 | A0A072UQ14 | 81.7 | 361 | 21 | 2 | 1 | 316 | 1 | 361 | 7.7e-162 | 579.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene56901.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56901.t1 | MTR_4g105130 | 82.271 | 361 | 19 | 2 | 1 | 316 | 1 | 361 | 0.0 | 576 |
| MS.gene56901.t1 | MTR_3g077110 | 45.103 | 388 | 102 | 16 | 1 | 316 | 1 | 349 | 1.84e-77 | 240 |
| MS.gene56901.t1 | MTR_1g112760 | 44.493 | 227 | 53 | 6 | 3 | 183 | 4 | 203 | 1.71e-47 | 159 |
| MS.gene56901.t1 | MTR_7g110830 | 57.576 | 132 | 14 | 1 | 1 | 90 | 1 | 132 | 7.86e-45 | 156 |
| MS.gene56901.t1 | MTR_1g085640 | 49.701 | 167 | 41 | 2 | 1 | 124 | 1 | 167 | 9.12e-44 | 154 |
| MS.gene56901.t1 | MTR_1g085040 | 53.191 | 141 | 24 | 1 | 1 | 99 | 1 | 141 | 5.36e-42 | 149 |
| MS.gene56901.t1 | MTR_7g111290 | 54.198 | 131 | 18 | 1 | 1 | 89 | 1 | 131 | 3.18e-34 | 129 |
| MS.gene56901.t1 | MTR_2g099740 | 48.000 | 125 | 23 | 1 | 1 | 83 | 1 | 125 | 1.47e-30 | 119 |
| MS.gene56901.t1 | MTR_2g064160 | 44.853 | 136 | 32 | 2 | 1 | 94 | 1 | 135 | 9.36e-30 | 115 |
| MS.gene56901.t1 | MTR_8g017440 | 46.774 | 124 | 24 | 1 | 1 | 82 | 1 | 124 | 2.36e-29 | 115 |
| MS.gene56901.t1 | MTR_8g017540 | 46.774 | 124 | 24 | 1 | 1 | 82 | 47 | 170 | 4.44e-29 | 115 |
| MS.gene56901.t1 | MTR_8g095390 | 44.186 | 129 | 30 | 1 | 1 | 87 | 1 | 129 | 1.57e-27 | 109 |
| MS.gene56901.t1 | MTR_8g017500 | 44.355 | 124 | 27 | 1 | 1 | 82 | 1 | 124 | 5.58e-27 | 108 |
| MS.gene56901.t1 | MTR_6g009430 | 37.121 | 132 | 41 | 1 | 1 | 90 | 1 | 132 | 5.79e-27 | 108 |
| MS.gene56901.t1 | MTR_4g478180 | 42.188 | 128 | 32 | 1 | 1 | 86 | 2 | 129 | 3.57e-26 | 106 |
| MS.gene56901.t1 | MTR_4g082230 | 41.406 | 128 | 33 | 1 | 1 | 86 | 1 | 128 | 5.51e-26 | 107 |
| MS.gene56901.t1 | MTR_3g074520 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 7.53e-26 | 105 |
| MS.gene56901.t1 | MTR_4g073420 | 39.394 | 132 | 38 | 1 | 1 | 90 | 1 | 132 | 8.24e-26 | 104 |
| MS.gene56901.t1 | MTR_8g098860 | 43.697 | 119 | 25 | 1 | 1 | 77 | 1 | 119 | 1.53e-25 | 104 |
| MS.gene56901.t1 | MTR_8g017340 | 40.816 | 147 | 44 | 2 | 1 | 104 | 1 | 147 | 2.02e-25 | 106 |
| MS.gene56901.t1 | MTR_3g097450 | 39.394 | 132 | 38 | 1 | 1 | 90 | 1 | 132 | 3.32e-25 | 102 |
| MS.gene56901.t1 | MTR_5g029840 | 40.458 | 131 | 36 | 1 | 1 | 89 | 1 | 131 | 3.58e-25 | 103 |
| MS.gene56901.t1 | MTR_8g017350 | 40.136 | 147 | 45 | 2 | 1 | 104 | 1 | 147 | 5.90e-25 | 105 |
| MS.gene56901.t1 | MTR_4g082290 | 41.406 | 128 | 33 | 1 | 1 | 86 | 1 | 128 | 8.78e-25 | 103 |
| MS.gene56901.t1 | MTR_8g017390 | 45.763 | 118 | 21 | 2 | 1 | 75 | 1 | 118 | 1.33e-24 | 103 |
| MS.gene56901.t1 | MTR_7g011170 | 39.706 | 136 | 40 | 1 | 1 | 94 | 1 | 136 | 1.54e-24 | 101 |
| MS.gene56901.t1 | MTR_5g078140 | 42.308 | 130 | 33 | 1 | 1 | 88 | 1 | 130 | 1.63e-24 | 101 |
| MS.gene56901.t1 | MTR_3g065440 | 40.441 | 136 | 39 | 1 | 1 | 94 | 1 | 136 | 2.09e-24 | 100 |
| MS.gene56901.t1 | MTR_6g012690 | 37.241 | 145 | 49 | 1 | 1 | 103 | 1 | 145 | 6.91e-24 | 100 |
| MS.gene56901.t1 | MTR_3g461490 | 38.776 | 147 | 44 | 3 | 1 | 105 | 1 | 143 | 1.58e-23 | 99.0 |
| MS.gene56901.t1 | MTR_2g089620 | 39.844 | 128 | 35 | 1 | 1 | 86 | 1 | 128 | 2.16e-23 | 98.6 |
| MS.gene56901.t1 | MTR_4g102380 | 40.310 | 129 | 35 | 1 | 1 | 87 | 1 | 129 | 2.18e-23 | 97.4 |
| MS.gene56901.t1 | MTR_2g011660 | 38.760 | 129 | 36 | 2 | 1 | 86 | 1 | 129 | 2.62e-23 | 98.6 |
| MS.gene56901.t1 | MTR_5g079670 | 38.400 | 125 | 35 | 1 | 3 | 85 | 2 | 126 | 3.58e-23 | 95.5 |
| MS.gene56901.t1 | MTR_5g010020 | 43.636 | 110 | 20 | 1 | 1 | 68 | 1 | 110 | 5.28e-23 | 97.8 |
| MS.gene56901.t1 | MTR_7g076740 | 41.221 | 131 | 34 | 2 | 1 | 88 | 1 | 131 | 7.17e-23 | 96.7 |
| MS.gene56901.t1 | MTR_5g007370 | 41.667 | 120 | 28 | 1 | 12 | 89 | 12 | 131 | 8.03e-23 | 96.7 |
| MS.gene56901.t1 | MTR_4g091490 | 37.984 | 129 | 37 | 2 | 1 | 86 | 1 | 129 | 8.95e-23 | 97.4 |
| MS.gene56901.t1 | MTR_3g083540 | 38.710 | 124 | 34 | 1 | 6 | 87 | 35 | 158 | 1.29e-21 | 93.2 |
| MS.gene56901.t1 | MTR_8g031360 | 40.000 | 135 | 37 | 3 | 1 | 91 | 1 | 135 | 1.64e-21 | 92.8 |
| MS.gene56901.t1 | MTR_2g097910 | 44.762 | 105 | 16 | 1 | 12 | 74 | 20 | 124 | 7.27e-21 | 91.3 |
| MS.gene56901.t1 | MTR_4g065017 | 44.340 | 106 | 17 | 1 | 11 | 74 | 16 | 121 | 1.45e-20 | 90.9 |
| MS.gene56901.t1 | MTR_4g485530 | 35.714 | 126 | 39 | 1 | 1 | 84 | 1 | 126 | 2.60e-20 | 87.4 |
| MS.gene56901.t1 | MTR_6g055910 | 44.545 | 110 | 19 | 1 | 9 | 76 | 11 | 120 | 5.64e-20 | 88.2 |
| MS.gene56901.t1 | MTR_3g028740 | 43.519 | 108 | 19 | 1 | 9 | 74 | 19 | 126 | 1.13e-19 | 88.6 |
| MS.gene56901.t1 | MTR_5g042030 | 37.607 | 117 | 31 | 1 | 12 | 86 | 7 | 123 | 1.95e-17 | 81.3 |
| MS.gene56901.t1 | MTR_8g017480 | 71.111 | 45 | 13 | 0 | 1 | 45 | 1 | 45 | 3.31e-17 | 74.7 |
| MS.gene56901.t1 | MTR_2g089450 | 35.593 | 118 | 34 | 1 | 1 | 76 | 1 | 118 | 2.40e-16 | 77.4 |
| MS.gene56901.t1 | MTR_3g045430 | 32.593 | 135 | 48 | 2 | 1 | 92 | 1 | 135 | 9.73e-15 | 73.9 |
| MS.gene56901.t1 | MTR_2g095520 | 36.937 | 111 | 27 | 2 | 1 | 68 | 1 | 111 | 1.49e-14 | 73.6 |
| MS.gene56901.t1 | MTR_7g111290 | 47.561 | 82 | 32 | 1 | 8 | 89 | 19 | 89 | 6.09e-13 | 68.9 |
| MS.gene56901.t1 | MTR_8g020490 | 27.363 | 201 | 98 | 3 | 10 | 162 | 6 | 206 | 6.27e-11 | 62.0 |
| MS.gene56901.t1 | MTR_6g055910 | 44.928 | 69 | 27 | 1 | 8 | 76 | 34 | 91 | 7.79e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56901.t1 | AT5G26660 | 50.307 | 163 | 39 | 1 | 1 | 121 | 1 | 163 | 2.63e-44 | 155 |
| MS.gene56901.t1 | AT4G01680 | 56.489 | 131 | 15 | 1 | 1 | 89 | 1 | 131 | 1.86e-42 | 147 |
| MS.gene56901.t1 | AT1G57560 | 48.780 | 164 | 37 | 3 | 1 | 122 | 1 | 159 | 3.86e-42 | 147 |
| MS.gene56901.t1 | AT1G57560 | 53.957 | 139 | 21 | 2 | 1 | 97 | 1 | 138 | 7.15e-42 | 147 |
| MS.gene56901.t1 | AT4G01680 | 56.489 | 131 | 15 | 1 | 1 | 89 | 1 | 131 | 1.08e-41 | 147 |
| MS.gene56901.t1 | AT4G01680 | 51.748 | 143 | 15 | 2 | 1 | 89 | 1 | 143 | 4.33e-38 | 138 |
| MS.gene56901.t1 | AT1G09540 | 52.273 | 132 | 21 | 1 | 1 | 90 | 1 | 132 | 5.01e-38 | 139 |
| MS.gene56901.t1 | AT3G12720 | 48.413 | 126 | 22 | 2 | 1 | 84 | 12 | 136 | 3.24e-30 | 116 |
| MS.gene56901.t1 | AT3G13890 | 47.863 | 117 | 19 | 1 | 1 | 75 | 1 | 117 | 9.65e-29 | 114 |
| MS.gene56901.t1 | AT3G02940 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 1.17e-28 | 112 |
| MS.gene56901.t1 | AT3G02940 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 2.36e-28 | 112 |
| MS.gene56901.t1 | AT3G02940 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 2.36e-28 | 112 |
| MS.gene56901.t1 | AT3G13890 | 44.444 | 126 | 19 | 1 | 1 | 75 | 1 | 126 | 2.30e-27 | 110 |
| MS.gene56901.t1 | AT5G16770 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 3.07e-27 | 108 |
| MS.gene56901.t1 | AT5G16770 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 3.07e-27 | 108 |
| MS.gene56901.t1 | AT3G61250 | 40.972 | 144 | 43 | 1 | 1 | 102 | 1 | 144 | 5.18e-27 | 107 |
| MS.gene56901.t1 | AT5G16770 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 6.38e-27 | 108 |
| MS.gene56901.t1 | AT5G16770 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 6.38e-27 | 108 |
| MS.gene56901.t1 | AT2G16720 | 33.511 | 188 | 74 | 3 | 1 | 140 | 1 | 185 | 8.78e-27 | 106 |
| MS.gene56901.t1 | AT4G34990 | 36.774 | 155 | 55 | 2 | 1 | 112 | 1 | 155 | 2.98e-26 | 105 |
| MS.gene56901.t1 | AT1G63910 | 42.188 | 128 | 32 | 1 | 1 | 86 | 1 | 128 | 4.59e-26 | 106 |
| MS.gene56901.t1 | AT4G38620 | 37.324 | 142 | 47 | 1 | 1 | 100 | 1 | 142 | 6.25e-26 | 104 |
| MS.gene56901.t1 | AT4G21440 | 40.625 | 128 | 34 | 1 | 1 | 86 | 1 | 128 | 1.47e-25 | 105 |
| MS.gene56901.t1 | AT4G05100 | 41.085 | 129 | 33 | 2 | 1 | 86 | 1 | 129 | 3.52e-25 | 103 |
| MS.gene56901.t1 | AT5G15310 | 40.299 | 134 | 38 | 1 | 1 | 92 | 1 | 134 | 5.56e-25 | 102 |
| MS.gene56901.t1 | AT5G15310 | 40.299 | 134 | 38 | 1 | 1 | 92 | 1 | 134 | 6.79e-25 | 102 |
| MS.gene56901.t1 | AT4G09460 | 35.570 | 149 | 49 | 2 | 1 | 107 | 1 | 144 | 7.71e-25 | 100 |
| MS.gene56901.t1 | AT4G22680 | 41.221 | 131 | 35 | 1 | 1 | 89 | 1 | 131 | 9.74e-25 | 100 |
| MS.gene56901.t1 | AT5G15310 | 40.299 | 134 | 38 | 1 | 1 | 92 | 1 | 134 | 1.19e-24 | 100 |
| MS.gene56901.t1 | AT4G12350 | 39.850 | 133 | 38 | 1 | 1 | 91 | 1 | 133 | 1.31e-24 | 101 |
| MS.gene56901.t1 | AT3G13540 | 32.447 | 188 | 80 | 4 | 6 | 149 | 17 | 201 | 1.82e-24 | 100 |
| MS.gene56901.t1 | AT1G22640 | 35.374 | 147 | 50 | 2 | 1 | 102 | 1 | 147 | 1.83e-24 | 100 |
| MS.gene56901.t1 | AT1G34670 | 39.098 | 133 | 39 | 1 | 1 | 91 | 1 | 133 | 1.98e-24 | 102 |
| MS.gene56901.t1 | AT1G66230 | 38.926 | 149 | 46 | 2 | 1 | 104 | 1 | 149 | 2.04e-24 | 100 |
| MS.gene56901.t1 | AT5G61420 | 37.879 | 132 | 40 | 1 | 1 | 90 | 1 | 132 | 3.02e-24 | 101 |
| MS.gene56901.t1 | AT5G56110 | 38.776 | 147 | 44 | 3 | 1 | 105 | 1 | 143 | 3.82e-24 | 100 |
| MS.gene56901.t1 | AT5G16600 | 37.415 | 147 | 50 | 1 | 1 | 105 | 1 | 147 | 6.68e-24 | 100 |
| MS.gene56901.t1 | AT1G18570 | 38.129 | 139 | 43 | 2 | 1 | 96 | 1 | 139 | 6.86e-24 | 100 |
| MS.gene56901.t1 | AT5G10280 | 38.346 | 133 | 40 | 1 | 1 | 91 | 1 | 133 | 1.02e-23 | 99.8 |
| MS.gene56901.t1 | AT4G28110 | 38.281 | 128 | 37 | 1 | 1 | 86 | 1 | 128 | 1.28e-23 | 98.2 |
| MS.gene56901.t1 | AT3G01140 | 40.157 | 127 | 34 | 1 | 1 | 85 | 1 | 127 | 1.86e-23 | 99.0 |
| MS.gene56901.t1 | AT5G07690 | 37.692 | 130 | 39 | 1 | 1 | 88 | 1 | 130 | 3.71e-23 | 98.2 |
| MS.gene56901.t1 | AT4G17785 | 40.458 | 131 | 35 | 2 | 1 | 88 | 1 | 131 | 6.85e-23 | 97.8 |
| MS.gene56901.t1 | AT4G17785 | 40.458 | 131 | 35 | 2 | 1 | 88 | 1 | 131 | 7.02e-23 | 97.4 |
| MS.gene56901.t1 | AT5G65230 | 39.695 | 131 | 37 | 1 | 1 | 89 | 1 | 131 | 8.67e-23 | 96.7 |
| MS.gene56901.t1 | AT1G35515 | 37.405 | 131 | 40 | 1 | 1 | 89 | 1 | 131 | 8.87e-23 | 94.4 |
| MS.gene56901.t1 | AT1G74430 | 39.552 | 134 | 39 | 1 | 1 | 92 | 1 | 134 | 2.32e-22 | 94.7 |
| MS.gene56901.t1 | AT5G60890 | 36.434 | 129 | 40 | 1 | 1 | 87 | 1 | 129 | 8.41e-22 | 93.6 |
| MS.gene56901.t1 | AT5G54230 | 39.062 | 128 | 36 | 1 | 1 | 86 | 1 | 128 | 2.15e-21 | 92.8 |
| MS.gene56901.t1 | AT3G01140 | 39.344 | 122 | 32 | 1 | 6 | 85 | 49 | 170 | 3.88e-21 | 93.2 |
| MS.gene56901.t1 | AT3G28470 | 35.583 | 163 | 52 | 2 | 1 | 121 | 1 | 152 | 5.03e-21 | 92.0 |
| MS.gene56901.t1 | AT5G07700 | 35.385 | 130 | 42 | 1 | 1 | 88 | 1 | 130 | 5.23e-21 | 92.0 |
| MS.gene56901.t1 | AT5G07700 | 35.385 | 130 | 42 | 1 | 1 | 88 | 1 | 130 | 5.23e-21 | 92.0 |
| MS.gene56901.t1 | AT5G26660 | 42.982 | 114 | 54 | 1 | 8 | 121 | 30 | 132 | 5.52e-21 | 91.7 |
| MS.gene56901.t1 | AT1G74080 | 35.556 | 135 | 45 | 1 | 1 | 93 | 1 | 135 | 2.13e-20 | 90.5 |
| MS.gene56901.t1 | AT5G14340 | 35.616 | 146 | 44 | 2 | 1 | 104 | 13 | 150 | 2.61e-20 | 89.4 |
| MS.gene56901.t1 | AT5G62320 | 32.468 | 154 | 55 | 2 | 2 | 106 | 3 | 156 | 5.73e-20 | 87.4 |
| MS.gene56901.t1 | AT3G48920 | 42.857 | 112 | 21 | 2 | 9 | 77 | 15 | 126 | 4.00e-19 | 85.5 |
| MS.gene56901.t1 | AT3G08500 | 41.667 | 108 | 21 | 1 | 9 | 74 | 27 | 134 | 1.98e-18 | 85.1 |
| MS.gene56901.t1 | AT5G12870 | 41.284 | 109 | 22 | 1 | 10 | 76 | 16 | 124 | 5.40e-18 | 82.8 |
| MS.gene56901.t1 | AT2G31180 | 30.055 | 183 | 77 | 3 | 1 | 132 | 1 | 183 | 7.25e-18 | 82.0 |
| MS.gene56901.t1 | AT1G18710 | 34.351 | 131 | 44 | 1 | 1 | 89 | 1 | 131 | 1.03e-17 | 81.6 |
| MS.gene56901.t1 | AT5G65790 | 36.232 | 138 | 40 | 3 | 1 | 95 | 1 | 133 | 1.63e-17 | 82.8 |
| MS.gene56901.t1 | AT5G57620 | 38.136 | 118 | 30 | 2 | 1 | 75 | 1 | 118 | 2.03e-17 | 82.0 |
| MS.gene56901.t1 | AT5G23000 | 38.136 | 118 | 30 | 2 | 1 | 75 | 1 | 118 | 2.33e-17 | 81.6 |
| MS.gene56901.t1 | AT5G52260 | 40.000 | 110 | 24 | 1 | 9 | 76 | 9 | 118 | 1.82e-16 | 78.2 |
| MS.gene56901.t1 | AT1G22640 | 39.175 | 97 | 45 | 2 | 8 | 101 | 4 | 89 | 5.49e-16 | 75.5 |
| MS.gene56901.t1 | AT1G74430 | 57.895 | 57 | 24 | 0 | 36 | 92 | 9 | 65 | 7.85e-16 | 75.1 |
| MS.gene56901.t1 | AT4G37780 | 37.705 | 122 | 32 | 3 | 1 | 78 | 1 | 122 | 1.76e-15 | 75.9 |
| MS.gene56901.t1 | AT5G61420 | 62.500 | 48 | 18 | 0 | 43 | 90 | 6 | 53 | 2.49e-15 | 75.5 |
| MS.gene56901.t1 | AT5G15310 | 63.830 | 47 | 17 | 0 | 46 | 92 | 9 | 55 | 3.62e-14 | 71.2 |
| MS.gene56901.t1 | AT4G28110 | 63.415 | 41 | 15 | 0 | 46 | 86 | 4 | 44 | 4.01e-14 | 70.5 |
| MS.gene56901.t1 | AT1G22640 | 49.153 | 59 | 27 | 1 | 46 | 101 | 16 | 74 | 4.87e-14 | 69.7 |
| MS.gene56901.t1 | AT4G26930 | 44.444 | 72 | 28 | 2 | 8 | 79 | 68 | 127 | 3.27e-11 | 63.9 |
| MS.gene56901.t1 | AT5G14340 | 49.153 | 59 | 22 | 1 | 46 | 104 | 10 | 60 | 5.71e-11 | 61.2 |
| MS.gene56901.t1 | AT1G71030 | 57.500 | 40 | 17 | 0 | 46 | 85 | 42 | 81 | 6.55e-11 | 60.8 |
Find 45 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTGAAAGTGATGTTAA+TGG | 0.241227 | 8.1:-18301696 | None:intergenic |
| GCTATAAAATCTGAAGATTA+TGG | 0.250747 | 8.1:+18301609 | MS.gene56901:CDS |
| TTCAATCACATAACTAGATT+TGG | 0.285364 | 8.1:+18300510 | MS.gene56901:CDS |
| CTAGATTTGGTGTTGGTTGC+TGG | 0.291906 | 8.1:+18300523 | MS.gene56901:CDS |
| TTCTGCTATGATTTCCAATC+TGG | 0.306161 | 8.1:+18301243 | MS.gene56901:CDS |
| TCGAGTTACTATGATGATAA+TGG | 0.318372 | 8.1:+18301165 | MS.gene56901:CDS |
| TTAAGATATGCTTCTGTAAG+AGG | 0.318967 | 8.1:-18301063 | None:intergenic |
| AATTACTTACCAGCTTGTTT+AGG | 0.360613 | 8.1:-18300555 | None:intergenic |
| GTAAGAGGCTTGTGTGTTGT+TGG | 0.393471 | 8.1:-18301048 | None:intergenic |
| GACTCTGATCACATGGTTTG+TGG | 0.425354 | 8.1:-18301299 | None:intergenic |
| CCATGTGATCAGAGTCAATT+CGG | 0.429235 | 8.1:+18301306 | MS.gene56901:CDS |
| AAGATTATGGAACTAGTTCA+TGG | 0.437284 | 8.1:+18301622 | MS.gene56901:CDS |
| AATTTACAATTCATATATGA+TGG | 0.446483 | 8.1:-18301746 | None:intergenic |
| TCATTGTTCATTAATGATCA+AGG | 0.452544 | 8.1:+18301438 | MS.gene56901:CDS |
| TTTCACCAACAAACAAGCAT+TGG | 0.454876 | 8.1:+18301212 | MS.gene56901:CDS |
| TAGGTAAGTTATAACCAGAT+TGG | 0.455802 | 8.1:-18301257 | None:intergenic |
| CAAATAGCAGCACAATTACC+AGG | 0.477311 | 8.1:+18300952 | MS.gene56901:CDS |
| ATGATGGAAAATAATAATGC+AGG | 0.477586 | 8.1:+18301528 | MS.gene56901:CDS |
| CACATAACTAGATTTGGTGT+TGG | 0.484451 | 8.1:+18300516 | MS.gene56901:CDS |
| TCTTACAGAAGCATATCTTA+AGG | 0.488204 | 8.1:+18301065 | MS.gene56901:CDS |
| TCAAATGAGAAGTACTATGA+TGG | 0.493294 | 8.1:+18301512 | MS.gene56901:CDS |
| AGGGTCCAATGCTTGTTTGT+TGG | 0.508884 | 8.1:-18301217 | None:intergenic |
| TTTAATTGGAACTGAAACAA+AGG | 0.509478 | 8.1:-18301585 | None:intergenic |
| ATCATCATAGTAACTCGAAT+CGG | 0.515891 | 8.1:-18301160 | None:intergenic |
| AAGAAGAAGCTTCTAAAGCA+AGG | 0.517547 | 8.1:+18301018 | MS.gene56901:CDS |
| TTAATTGGAACTGAAACAAA+GGG | 0.517731 | 8.1:-18301584 | None:intergenic |
| ATAGTACTTCTCATTTGAGA+TGG | 0.517884 | 8.1:-18301507 | None:intergenic |
| GGAAATCATAGCAGAACAAA+GGG | 0.529276 | 8.1:-18301236 | None:intergenic |
| ATTTCATTATCTGTTCTCCC+TGG | 0.534130 | 8.1:-18300970 | None:intergenic |
| GGAACTAGTTCATGGGAAGA+TGG | 0.535936 | 8.1:+18301630 | MS.gene56901:CDS |
| CCGAATTGACTCTGATCACA+TGG | 0.550295 | 8.1:-18301306 | None:intergenic |
| TGATCTGAATTTGTTAGACT+CGG | 0.559414 | 8.1:-18301360 | None:intergenic |
| CTTGTGTGATCATTACTCAT+AGG | 0.561692 | 8.1:-18301276 | None:intergenic |
| AGAAGAAGCTTCTAAAGCAA+GGG | 0.563543 | 8.1:+18301019 | MS.gene56901:CDS |
| CATTGTTCATTAATGATCAA+GGG | 0.568836 | 8.1:+18301439 | MS.gene56901:CDS |
| TGATGGAAAATAATAATGCA+GGG | 0.573481 | 8.1:+18301529 | MS.gene56901:CDS |
| TGGAAATCATAGCAGAACAA+AGG | 0.583893 | 8.1:-18301237 | None:intergenic |
| AGTTCTGTTCCTAAACAAGC+TGG | 0.588563 | 8.1:+18300546 | MS.gene56901:CDS |
| TGTGATGGTATTTGCATAGG+TGG | 0.620021 | 8.1:-18301117 | None:intergenic |
| CCATGTGATGGTATTTGCAT+AGG | 0.627759 | 8.1:-18301120 | None:intergenic |
| AGATTATGGAACTAGTTCAT+GGG | 0.627814 | 8.1:+18301623 | MS.gene56901:CDS |
| GTTGTCCGAAAACTCTGTCA+CGG | 0.647107 | 8.1:-18301394 | None:intergenic |
| CCTATGCAAATACCATCACA+TGG | 0.666893 | 8.1:+18301120 | MS.gene56901:CDS |
| AGAAATGCTGAACCATGTGA+TGG | 0.672145 | 8.1:-18301132 | None:intergenic |
| AAATAGCAGCACAATTACCA+GGG | 0.695212 | 8.1:+18300953 | MS.gene56901:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATGAAATTAAAAACTTT+TGG | + | chr8.1:18300983-18301002 | MS.gene56901:CDS | 10.0% |
| !! | GTAAATTTGATAAAATCACA+AGG | - | chr8.1:18300866-18300885 | None:intergenic | 20.0% |
| !!! | CAGATTTTATAGCATTTAAT+TGG | - | chr8.1:18301602-18301621 | None:intergenic | 20.0% |
| !!! | TTTCTTTTTTCGAACATTAT+AGG | + | chr8.1:18300923-18300942 | MS.gene56901:intron | 20.0% |
| ! | AAAAATTGAGGAAAGGTTTA+TGG | + | chr8.1:18300463-18300482 | MS.gene56901:CDS | 25.0% |
| ! | CATTGTTCATTAATGATCAA+GGG | + | chr8.1:18301439-18301458 | MS.gene56901:CDS | 25.0% |
| ! | GATTCAATATATATCGTGTT+TGG | + | chr8.1:18300809-18300828 | MS.gene56901:intron | 25.0% |
| ! | GCTATAAAATCTGAAGATTA+TGG | + | chr8.1:18301609-18301628 | MS.gene56901:CDS | 25.0% |
| ! | TCATTGTTCATTAATGATCA+AGG | + | chr8.1:18301438-18301457 | MS.gene56901:CDS | 25.0% |
| ! | TGATGGAAAATAATAATGCA+GGG | + | chr8.1:18301529-18301548 | MS.gene56901:CDS | 25.0% |
| ! | TTAAAGCAAAAATTGAGGAA+AGG | + | chr8.1:18300456-18300475 | MS.gene56901:CDS | 25.0% |
| ! | TTAATTGGAACTGAAACAAA+GGG | - | chr8.1:18301587-18301606 | None:intergenic | 25.0% |
| ! | TTCAATCACATAACTAGATT+TGG | + | chr8.1:18300510-18300529 | MS.gene56901:CDS | 25.0% |
| ! | TTTAATTGGAACTGAAACAA+AGG | - | chr8.1:18301588-18301607 | None:intergenic | 25.0% |
| !! | ATGATGGAAAATAATAATGC+AGG | + | chr8.1:18301528-18301547 | MS.gene56901:CDS | 25.0% |
| !! | GTTGTTTAAAGCAAAAATTG+AGG | + | chr8.1:18300451-18300470 | MS.gene56901:CDS | 25.0% |
| !!! | TTTTTTCGAACATTATAGGT+GGG | + | chr8.1:18300927-18300946 | MS.gene56901:intron | 25.0% |
| AACATTCCTCTCTTTAAATC+AGG | - | chr8.1:18300696-18300715 | None:intergenic | 30.0% | |
| AAGAAATATGTTTGGATTCG+CGG | - | chr8.1:18300888-18300907 | None:intergenic | 30.0% | |
| AAGATTATGGAACTAGTTCA+TGG | + | chr8.1:18301622-18301641 | MS.gene56901:CDS | 30.0% | |
| AGATTATGGAACTAGTTCAT+GGG | + | chr8.1:18301623-18301642 | MS.gene56901:CDS | 30.0% | |
| ATAGTACTTCTCATTTGAGA+TGG | - | chr8.1:18301510-18301529 | None:intergenic | 30.0% | |
| ATCATCATAGTAACTCGAAT+CGG | - | chr8.1:18301163-18301182 | None:intergenic | 30.0% | |
| CGTAACTGAAGAAATATGTT+TGG | - | chr8.1:18300896-18300915 | None:intergenic | 30.0% | |
| TAGGTAAGTTATAACCAGAT+TGG | - | chr8.1:18301260-18301279 | None:intergenic | 30.0% | |
| TCAAAACTAAGAGTCTAACA+TGG | + | chr8.1:18300599-18300618 | MS.gene56901:intron | 30.0% | |
| TCGAGTTACTATGATGATAA+TGG | + | chr8.1:18301165-18301184 | MS.gene56901:CDS | 30.0% | |
| TGAGATGGATAAACTATTTG+AGG | + | chr8.1:18300670-18300689 | MS.gene56901:intron | 30.0% | |
| TTAAGATATGCTTCTGTAAG+AGG | - | chr8.1:18301066-18301085 | None:intergenic | 30.0% | |
| ! | AAAAGCTTTTCATCTTCTTC+AGG | - | chr8.1:18300492-18300511 | None:intergenic | 30.0% |
| ! | AATTACTTACCAGCTTGTTT+AGG | - | chr8.1:18300558-18300577 | None:intergenic | 30.0% |
| ! | ATATATCGTGTTTGGAATCA+CGG | + | chr8.1:18300817-18300836 | MS.gene56901:intron | 30.0% |
| ! | TCTTACAGAAGCATATCTTA+AGG | + | chr8.1:18301065-18301084 | MS.gene56901:CDS | 30.0% |
| ! | TCTTCTGAAAGTGATGTTAA+TGG | - | chr8.1:18301699-18301718 | None:intergenic | 30.0% |
| !! | ATAATAATGCAGGGTTTTGT+TGG | + | chr8.1:18301538-18301557 | MS.gene56901:CDS | 30.0% |
| !! | ATGGTTGATGAAATTCTTTC+AGG | + | chr8.1:18300618-18300637 | MS.gene56901:intron | 30.0% |
| !! | TCAAATGAGAAGTACTATGA+TGG | + | chr8.1:18301512-18301531 | MS.gene56901:CDS | 30.0% |
| !! | TGATCTGAATTTGTTAGACT+CGG | - | chr8.1:18301363-18301382 | None:intergenic | 30.0% |
| !!! | CTTTTTTCGAACATTATAGG+TGG | + | chr8.1:18300926-18300945 | MS.gene56901:intron | 30.0% |
| !!! | TAATAATGCAGGGTTTTGTT+GGG | + | chr8.1:18301539-18301558 | MS.gene56901:CDS | 30.0% |
| AAATAGCAGCACAATTACCA+GGG | + | chr8.1:18300953-18300972 | MS.gene56901:CDS | 35.0% | |
| ATTTCATTATCTGTTCTCCC+TGG | - | chr8.1:18300973-18300992 | None:intergenic | 35.0% | |
| CCTTATGTTACCTATTTCCA+AGG | - | chr8.1:18300761-18300780 | None:intergenic | 35.0% | |
| CCTTGGAAATAGGTAACATA+AGG | + | chr8.1:18300758-18300777 | MS.gene56901:intron | 35.0% | |
| CTTGTGTGATCATTACTCAT+AGG | - | chr8.1:18301279-18301298 | None:intergenic | 35.0% | |
| GAAAGAGTTGTAGATTGAGA+TGG | + | chr8.1:18300655-18300674 | MS.gene56901:intron | 35.0% | |
| GGAAATCATAGCAGAACAAA+GGG | - | chr8.1:18301239-18301258 | None:intergenic | 35.0% | |
| TGGAAATCATAGCAGAACAA+AGG | - | chr8.1:18301240-18301259 | None:intergenic | 35.0% | |
| TTCATGAAGTCCTTGGAAAT+AGG | + | chr8.1:18300748-18300767 | MS.gene56901:intron | 35.0% | |
| TTCTGCTATGATTTCCAATC+TGG | + | chr8.1:18301243-18301262 | MS.gene56901:CDS | 35.0% | |
| TTTCACCAACAAACAAGCAT+TGG | + | chr8.1:18301212-18301231 | MS.gene56901:CDS | 35.0% | |
| !! | AAGAAGAAGCTTCTAAAGCA+AGG | + | chr8.1:18301018-18301037 | MS.gene56901:CDS | 35.0% |
| !! | AGAAGAAGCTTCTAAAGCAA+GGG | + | chr8.1:18301019-18301038 | MS.gene56901:CDS | 35.0% |
| !! | CACATAACTAGATTTGGTGT+TGG | + | chr8.1:18300516-18300535 | MS.gene56901:CDS | 35.0% |
| AATGTTCTCACAGCAAGAAG+AGG | + | chr8.1:18300710-18300729 | MS.gene56901:intron | 40.0% | |
| AGAAATGCTGAACCATGTGA+TGG | - | chr8.1:18301135-18301154 | None:intergenic | 40.0% | |
| AGTTCTGTTCCTAAACAAGC+TGG | + | chr8.1:18300546-18300565 | MS.gene56901:CDS | 40.0% | |
| ATCAGTCTTCATGAAGTCCT+TGG | + | chr8.1:18300741-18300760 | MS.gene56901:intron | 40.0% | |
| CAAATAGCAGCACAATTACC+AGG | + | chr8.1:18300952-18300971 | MS.gene56901:CDS | 40.0% | |
| CCATGTGATCAGAGTCAATT+CGG | + | chr8.1:18301306-18301325 | MS.gene56901:CDS | 40.0% | |
| CCATGTGATGGTATTTGCAT+AGG | - | chr8.1:18301123-18301142 | None:intergenic | 40.0% | |
| CCTATGCAAATACCATCACA+TGG | + | chr8.1:18301120-18301139 | MS.gene56901:CDS | 40.0% | |
| CTTTCAGGACTTCAAAGATG+TGG | + | chr8.1:18300633-18300652 | MS.gene56901:intron | 40.0% | |
| TGTGATGGTATTTGCATAGG+TGG | - | chr8.1:18301120-18301139 | None:intergenic | 40.0% | |
| TTGAGGCCTGATTTAAAGAG+AGG | + | chr8.1:18300687-18300706 | MS.gene56901:intron | 40.0% | |
| AGGGTCCAATGCTTGTTTGT+TGG | - | chr8.1:18301220-18301239 | None:intergenic | 45.0% | |
| GACTCTGATCACATGGTTTG+TGG | - | chr8.1:18301302-18301321 | None:intergenic | 45.0% | |
| GGAACTAGTTCATGGGAAGA+TGG | + | chr8.1:18301630-18301649 | MS.gene56901:CDS | 45.0% | |
| GTAAGAGGCTTGTGTGTTGT+TGG | - | chr8.1:18301051-18301070 | None:intergenic | 45.0% | |
| GTTGTCCGAAAACTCTGTCA+CGG | - | chr8.1:18301397-18301416 | None:intergenic | 45.0% | |
| ! | CCGAATTGACTCTGATCACA+TGG | - | chr8.1:18301309-18301328 | None:intergenic | 45.0% |
| ! | TGTGTCCGTGACAGAGTTTT+CGG | + | chr8.1:18301389-18301408 | MS.gene56901:CDS | 45.0% |
| !! | CTAGATTTGGTGTTGGTTGC+TGG | + | chr8.1:18300523-18300542 | MS.gene56901:CDS | 45.0% |
| !!! | AATGCAGGGTTTTGTTGGGA+TGG | + | chr8.1:18301543-18301562 | MS.gene56901:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 18300435 | 18301758 | 18300435 | ID=MS.gene56901 |
| chr8.1 | mRNA | 18300435 | 18301758 | 18300435 | ID=MS.gene56901.t1;Parent=MS.gene56901 |
| chr8.1 | exon | 18300435 | 18300571 | 18300435 | ID=MS.gene56901.t1.exon1;Parent=MS.gene56901.t1 |
| chr8.1 | CDS | 18300435 | 18300571 | 18300435 | ID=cds.MS.gene56901.t1;Parent=MS.gene56901.t1 |
| chr8.1 | exon | 18300945 | 18301758 | 18300945 | ID=MS.gene56901.t1.exon2;Parent=MS.gene56901.t1 |
| chr8.1 | CDS | 18300945 | 18301758 | 18300945 | ID=cds.MS.gene56901.t1;Parent=MS.gene56901.t1 |
| Gene Sequence |
| Protein sequence |
