Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57245.t1 | AES59248.1 | 89.1 | 55 | 6 | 0 | 1 | 55 | 50 | 104 | 3.60E-20 | 107.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57245.t1 | G7I9Y7 | 89.1 | 55 | 6 | 0 | 1 | 55 | 50 | 104 | 2.6e-20 | 107.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene57245.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57245.t1 | MTR_1g016200 | 89.091 | 55 | 6 | 0 | 1 | 55 | 50 | 104 | 3.77e-32 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATGAGAGTAAGCAATATT+TGG | 0.256438 | 1.4:+6144242 | None:intergenic |
| TTGAGTATCAAGACCCTTAT+CGG | 0.292091 | 1.4:-6144513 | None:intergenic |
| ATACAAATACTACTTCTCTT+TGG | 0.332697 | 1.4:+6144293 | MS.gene57245:CDS |
| TCTTGATACTCAAAAGGAAA+AGG | 0.439670 | 1.4:+6144523 | MS.gene57245:CDS |
| TTTCCTCTTCCCTTCTTCGT+CGG | 0.486411 | 1.4:-6144487 | None:intergenic |
| ACTCAAAAGGAAAAGGGAAA+TGG | 0.486925 | 1.4:+6144530 | MS.gene57245:CDS |
| CAAATACTACTTCTCTTTGG+AGG | 0.495243 | 1.4:+6144296 | MS.gene57245:CDS |
| TAAGGGTCTTGATACTCAAA+AGG | 0.499945 | 1.4:+6144517 | MS.gene57245:CDS |
| CTTGATACTCAAAAGGAAAA+GGG | 0.500087 | 1.4:+6144524 | MS.gene57245:CDS |
| TCTTTGGAGGAGTGATCCTA+CGG | 0.509509 | 1.4:+6144309 | MS.gene57245:CDS |
| ATGTGGACTCCGACGAAGAA+GGG | 0.537059 | 1.4:+6144478 | MS.gene57245:CDS |
| GAGTGATCCTACGGGTTCCA+AGG | 0.561497 | 1.4:+6144318 | MS.gene57245:CDS |
| CTTTGGAGGAGTGATCCTAC+GGG | 0.578542 | 1.4:+6144310 | MS.gene57245:CDS |
| CATGTGGACTCCGACGAAGA+AGG | 0.582374 | 1.4:+6144477 | MS.gene57245:CDS |
| TGTATCGCAATCGAAACAAA+CGG | 0.590406 | 1.4:-6144275 | None:intergenic |
| ACTCCGACGAAGAAGGGAAG+AGG | 0.605566 | 1.4:+6144484 | MS.gene57245:CDS |
| TGGCGAAAGCTCATCATCTG+AGG | 0.622295 | 1.4:+6144550 | MS.gene57245:CDS |
| CAGTGGTTGTGTAACGCATG+TGG | 0.661654 | 1.4:+6144461 | MS.gene57245:intron |
| CTAATTACCTTGGAACCCGT+AGG | 0.693638 | 1.4:-6144325 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGTTAACAAGAAATATGT+TGG | + | chr1.4:6144436-6144455 | MS.gene57245:intron | 20.0% |
| ! | ATACAAATACTACTTCTCTT+TGG | + | chr1.4:6144293-6144312 | MS.gene57245:CDS | 25.0% |
| !!! | CTACTATCAACTTTAACTTT+TGG | + | chr1.4:6144361-6144380 | MS.gene57245:intron | 25.0% |
| AAGAGATGAACTAATTACCT+TGG | - | chr1.4:6144338-6144357 | None:intergenic | 30.0% | |
| CTTGATACTCAAAAGGAAAA+GGG | + | chr1.4:6144524-6144543 | MS.gene57245:CDS | 30.0% | |
| TCTTGATACTCAAAAGGAAA+AGG | + | chr1.4:6144523-6144542 | MS.gene57245:CDS | 30.0% | |
| ACTCAAAAGGAAAAGGGAAA+TGG | + | chr1.4:6144530-6144549 | MS.gene57245:CDS | 35.0% | |
| CAAATACTACTTCTCTTTGG+AGG | + | chr1.4:6144296-6144315 | MS.gene57245:CDS | 35.0% | |
| TAAGGGTCTTGATACTCAAA+AGG | + | chr1.4:6144517-6144536 | MS.gene57245:CDS | 35.0% | |
| TGTATCGCAATCGAAACAAA+CGG | - | chr1.4:6144278-6144297 | None:intergenic | 35.0% | |
| TTGAGTATCAAGACCCTTAT+CGG | - | chr1.4:6144516-6144535 | None:intergenic | 35.0% | |
| CAAGAAATATGTTGGTGCAG+TGG | + | chr1.4:6144444-6144463 | MS.gene57245:intron | 40.0% | |
| GAAGAGGAAAAAGCCGATAA+GGG | + | chr1.4:6144500-6144519 | MS.gene57245:CDS | 40.0% | |
| CTAATTACCTTGGAACCCGT+AGG | - | chr1.4:6144328-6144347 | None:intergenic | 45.0% | |
| GGAAGAGGAAAAAGCCGATA+AGG | + | chr1.4:6144499-6144518 | MS.gene57245:CDS | 45.0% | |
| TTTCCTCTTCCCTTCTTCGT+CGG | - | chr1.4:6144490-6144509 | None:intergenic | 45.0% | |
| ! | TCTTTGGAGGAGTGATCCTA+CGG | + | chr1.4:6144309-6144328 | MS.gene57245:CDS | 45.0% |
| ATGTGGACTCCGACGAAGAA+GGG | + | chr1.4:6144478-6144497 | MS.gene57245:CDS | 50.0% | |
| CAGTGGTTGTGTAACGCATG+TGG | + | chr1.4:6144461-6144480 | MS.gene57245:intron | 50.0% | |
| TGGCGAAAGCTCATCATCTG+AGG | + | chr1.4:6144550-6144569 | MS.gene57245:CDS | 50.0% | |
| ! | CTTTGGAGGAGTGATCCTAC+GGG | + | chr1.4:6144310-6144329 | MS.gene57245:CDS | 50.0% |
| ACTCCGACGAAGAAGGGAAG+AGG | + | chr1.4:6144484-6144503 | MS.gene57245:CDS | 55.0% | |
| CATGTGGACTCCGACGAAGA+AGG | + | chr1.4:6144477-6144496 | MS.gene57245:CDS | 55.0% | |
| GAGTGATCCTACGGGTTCCA+AGG | + | chr1.4:6144318-6144337 | MS.gene57245:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 6144244 | 6144589 | 6144244 | ID=MS.gene57245 |
| chr1.4 | mRNA | 6144244 | 6144589 | 6144244 | ID=MS.gene57245.t1;Parent=MS.gene57245 |
| chr1.4 | exon | 6144244 | 6144339 | 6144244 | ID=MS.gene57245.t1.exon1;Parent=MS.gene57245.t1 |
| chr1.4 | CDS | 6144244 | 6144339 | 6144244 | ID=cds.MS.gene57245.t1;Parent=MS.gene57245.t1 |
| chr1.4 | exon | 6144464 | 6144589 | 6144464 | ID=MS.gene57245.t1.exon2;Parent=MS.gene57245.t1 |
| chr1.4 | CDS | 6144464 | 6144589 | 6144464 | ID=cds.MS.gene57245.t1;Parent=MS.gene57245.t1 |
| Gene Sequence |
| Protein sequence |
