AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57393

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57393.t1 MTR_8g093790 91.892 185 8 3 1 178 1 185 4.59e-119 334
MS.gene57393.t1 MTR_3g096400 51.220 205 67 9 7 178 4 208 2.58e-56 176
MS.gene57393.t1 MTR_4g094302 46.032 189 74 6 10 178 11 191 1.66e-48 156
MS.gene57393.t1 MTR_4g094302 49.167 120 51 3 10 119 11 130 2.73e-35 121
MS.gene57393.t1 MTR_4g081870 38.021 192 92 7 2 169 1 189 1.52e-30 112
MS.gene57393.t1 MTR_2g080010 37.017 181 81 5 10 157 15 195 1.18e-29 111
MS.gene57393.t1 MTR_8g094580 43.038 158 67 6 5 139 2 159 2.61e-29 109
MS.gene57393.t1 MTR_7g005280 40.854 164 72 7 8 150 13 172 2.00e-28 106
MS.gene57393.t1 MTR_5g041940 37.222 180 88 6 8 162 1 180 2.04e-28 106
MS.gene57393.t1 MTR_2157s0010 34.946 186 88 6 4 157 2 186 4.28e-28 105
MS.gene57393.t1 MTR_3g088110 37.430 179 90 6 6 162 3 181 1.88e-27 104
MS.gene57393.t1 MTR_2g093810 36.257 171 83 5 3 147 7 177 3.86e-26 102
MS.gene57393.t1 MTR_7g100990 38.728 173 79 7 6 152 7 178 5.40e-26 101
MS.gene57393.t1 MTR_8g059170 40.244 164 68 7 6 143 10 169 4.15e-25 99.4
MS.gene57393.t1 MTR_4g089135 37.288 177 66 6 10 141 15 191 4.23e-25 99.4
MS.gene57393.t1 MTR_3g096920 39.490 157 73 6 5 139 2 158 4.28e-25 98.6
MS.gene57393.t1 MTR_2g079990 38.608 158 77 5 6 143 12 169 5.84e-25 99.4
MS.gene57393.t1 MTR_3g435150 37.423 163 80 5 10 150 16 178 1.10e-24 97.8
MS.gene57393.t1 MTR_7g085220 37.647 170 72 7 2 138 1 169 1.33e-24 98.2
MS.gene57393.t1 MTR_6g084430 38.667 150 74 5 10 141 5 154 4.71e-24 96.3
MS.gene57393.t1 MTR_4g108760 39.073 151 70 5 10 138 17 167 7.13e-24 96.3
MS.gene57393.t1 MTR_4g035590 34.031 191 101 8 4 169 2 192 6.01e-23 94.0
MS.gene57393.t1 MTR_8g099750 35.443 158 77 5 9 141 13 170 3.29e-22 89.7
MS.gene57393.t1 MTR_3g098810 37.037 162 74 6 9 142 13 174 3.84e-22 89.7
MS.gene57393.t1 MTR_3g109340 36.310 168 87 5 10 157 22 189 4.86e-22 91.3
MS.gene57393.t1 MTR_2g062730 34.706 170 89 7 1 148 1 170 6.25e-22 90.9
MS.gene57393.t1 MTR_1g008740 39.103 156 73 5 5 138 22 177 6.58e-22 91.3
MS.gene57393.t1 MTR_3g109340 35.503 169 88 5 10 157 22 190 9.03e-22 90.5
MS.gene57393.t1 MTR_1g008740 39.103 156 73 5 5 138 8 163 9.71e-22 90.5
MS.gene57393.t1 MTR_3g064580 38.312 154 72 6 8 138 4 157 2.54e-21 90.5
MS.gene57393.t1 MTR_3g070030 37.059 170 81 7 8 154 17 183 3.09e-21 89.0
MS.gene57393.t1 MTR_7g085260 32.338 201 102 6 3 177 10 202 4.90e-21 89.0
MS.gene57393.t1 MTR_3g096920 39.394 132 65 4 5 121 2 133 4.91e-21 88.2
MS.gene57393.t1 MTR_1g069805 36.538 156 81 5 8 145 8 163 5.26e-21 87.4
MS.gene57393.t1 MTR_2g079990 32.796 186 77 5 6 143 12 197 8.62e-21 88.2
MS.gene57393.t1 MTR_2g064470 37.255 153 71 7 8 138 9 158 9.37e-21 87.0
MS.gene57393.t1 MTR_6g012670 33.728 169 90 5 4 150 14 182 1.50e-20 87.0
MS.gene57393.t1 MTR_7g097090 37.415 147 74 5 8 136 8 154 2.85e-20 85.9
MS.gene57393.t1 MTR_6g477900 36.774 155 76 6 6 138 2 156 6.39e-20 84.0
MS.gene57393.t1 MTR_5g021710 32.632 190 100 8 10 173 8 195 8.29e-20 85.1
MS.gene57393.t1 MTR_8g076110 35.758 165 81 8 10 151 6 168 1.25e-19 84.3
MS.gene57393.t1 MTR_2g064090 32.418 182 98 8 6 162 2 183 1.28e-19 84.7
MS.gene57393.t1 MTR_5g076850 37.179 156 74 7 6 138 2 156 1.90e-19 85.1
MS.gene57393.t1 MTR_4g036030 32.984 191 103 8 10 178 7 194 2.49e-19 84.0
MS.gene57393.t1 MTR_5g012080 34.839 155 78 6 10 141 7 161 2.50e-19 84.0
MS.gene57393.t1 MTR_1g090720 34.395 157 75 6 10 138 42 198 2.69e-19 84.7
MS.gene57393.t1 MTR_3g070040 37.908 153 74 6 8 139 6 158 3.77e-19 83.2
MS.gene57393.t1 MTR_3g098810 39.695 131 61 4 9 121 13 143 3.87e-19 80.5
MS.gene57393.t1 MTR_2g078700 37.908 153 71 6 10 138 23 175 4.71e-19 83.6
MS.gene57393.t1 MTR_6g011860 34.146 164 74 7 10 141 15 176 1.74e-18 82.0
MS.gene57393.t1 MTR_1g096430 36.478 159 76 8 2 138 1 156 1.21e-17 79.0
MS.gene57393.t1 MTR_7g011120 34.783 161 76 6 8 139 6 166 1.52e-17 78.6
MS.gene57393.t1 MTR_4g101680 33.333 153 81 6 10 141 7 159 1.70e-17 79.0
MS.gene57393.t1 MTR_2g068880 30.120 166 80 6 12 141 24 189 2.57e-17 77.8
MS.gene57393.t1 MTR_3g096140 37.333 150 72 8 12 139 9 158 3.03e-17 77.4
MS.gene57393.t1 MTR_8g024480 31.902 163 79 7 9 139 14 176 3.10e-17 78.6
MS.gene57393.t1 MTR_5g040420 33.125 160 77 6 12 141 16 175 3.29e-17 78.2
MS.gene57393.t1 MTR_8g467490 32.278 158 76 6 12 138 16 173 5.21e-17 77.8
MS.gene57393.t1 MTR_3g096140 37.333 150 72 8 12 139 9 158 5.92e-17 77.4
MS.gene57393.t1 MTR_4g134460 35.570 149 71 6 12 138 56 201 6.94e-17 77.4
MS.gene57393.t1 MTR_6g032770 34.591 159 80 5 4 138 12 170 8.19e-17 77.0
MS.gene57393.t1 MTR_3g116070 37.086 151 73 5 10 141 23 170 9.88e-17 76.6
MS.gene57393.t1 MTR_2g086880 36.111 144 66 8 12 135 20 157 1.16e-16 77.0
MS.gene57393.t1 MTR_5g090970 28.659 164 85 5 12 143 20 183 1.28e-16 75.9
MS.gene57393.t1 MTR_8g023840 34.932 146 74 6 10 135 11 155 2.13e-16 75.9
MS.gene57393.t1 MTR_7g011130 34.132 167 75 6 8 139 17 183 2.62e-16 75.5
MS.gene57393.t1 MTR_2g086880 37.594 133 61 7 12 124 20 150 2.99e-16 75.9
MS.gene57393.t1 MTR_4g075980 34.545 165 76 8 12 149 17 176 6.29e-16 73.9
MS.gene57393.t1 MTR_8g023930 30.952 168 92 8 10 157 24 187 7.91e-16 74.7
MS.gene57393.t1 MTR_1g090723 32.075 159 86 6 5 141 3 161 1.91e-15 73.6
MS.gene57393.t1 MTR_1g090723 32.075 159 86 6 5 141 3 161 2.11e-15 73.6
MS.gene57393.t1 MTR_1g090723 31.875 160 86 6 5 141 3 162 2.82e-15 73.2
MS.gene57393.t1 MTR_5g014300 31.847 157 81 6 8 138 4 160 4.32e-15 72.0
MS.gene57393.t1 MTR_4g098630 30.939 181 100 7 8 163 4 184 4.77e-15 71.6
MS.gene57393.t1 MTR_8g093580 32.680 153 81 7 9 139 5 157 8.07e-15 71.6
MS.gene57393.t1 MTR_8g063550 32.692 156 77 8 9 139 5 157 2.97e-14 70.1
MS.gene57393.t1 MTR_8g063550 32.692 156 77 8 9 139 5 157 4.02e-14 69.7
MS.gene57393.t1 MTR_8g063550 32.692 156 77 8 9 139 5 157 7.57e-14 68.6
MS.gene57393.t1 MTR_8g023860 31.507 146 79 6 10 135 12 156 8.93e-14 68.6
MS.gene57393.t1 MTR_8g023880 31.928 166 78 6 5 138 2 164 5.44e-13 65.5
MS.gene57393.t1 MTR_1g097300 34.127 126 65 5 12 119 21 146 1.56e-11 62.4
MS.gene57393.t1 MTR_4g052620 32.051 156 77 8 10 136 5 160 4.93e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57393.t1 AT5G64530 46.465 198 67 7 8 177 1 187 4.09e-50 160
MS.gene57393.t1 AT5G64530 51.261 119 50 3 8 119 1 118 5.34e-36 123
MS.gene57393.t1 AT1G77450 42.236 161 71 6 1 139 1 161 6.52e-29 107
MS.gene57393.t1 AT1G61110 41.317 167 65 6 10 143 16 182 9.65e-29 108
MS.gene57393.t1 AT2G33480 41.667 156 70 6 8 145 13 165 1.59e-28 107
MS.gene57393.t1 AT2G33480 41.772 158 69 7 8 145 13 167 2.47e-28 106
MS.gene57393.t1 AT1G69490 37.766 188 85 7 2 165 1 180 1.40e-27 104
MS.gene57393.t1 AT1G01720 42.581 155 66 6 8 139 5 159 1.05e-26 102
MS.gene57393.t1 AT5G39610 39.130 161 76 5 1 139 11 171 1.82e-26 102
MS.gene57393.t1 AT3G04070 37.912 182 79 8 1 150 1 180 3.50e-26 102
MS.gene57393.t1 AT1G52890 37.278 169 82 6 6 154 10 174 1.15e-25 100
MS.gene57393.t1 AT3G18400 38.065 155 77 5 10 145 5 159 2.47e-25 99.8
MS.gene57393.t1 AT3G15500 35.673 171 90 5 6 156 10 180 2.64e-25 99.8
MS.gene57393.t1 AT5G13180 39.241 158 66 7 8 141 12 163 3.23e-25 98.2
MS.gene57393.t1 AT5G63790 39.623 159 72 6 5 139 45 203 7.15e-25 98.6
MS.gene57393.t1 AT5G63790 39.623 159 72 6 5 139 55 213 1.09e-24 98.2
MS.gene57393.t1 AT3G12977 33.721 172 91 6 8 158 16 185 2.58e-24 96.3
MS.gene57393.t1 AT4G27410 36.585 205 97 10 6 178 10 213 4.27e-24 95.9
MS.gene57393.t1 AT2G24430 40.000 150 69 5 10 138 16 165 6.18e-24 95.9
MS.gene57393.t1 AT2G24430 40.000 150 69 5 10 138 16 165 6.18e-24 95.9
MS.gene57393.t1 AT5G61430 37.821 156 75 5 5 138 11 166 7.62e-24 95.9
MS.gene57393.t1 AT3G04070 34.848 198 79 8 1 150 1 196 8.36e-24 96.3
MS.gene57393.t1 AT5G07680 39.073 151 70 5 10 138 17 167 1.40e-23 95.1
MS.gene57393.t1 AT5G07680 39.073 151 70 5 10 138 3 153 1.43e-23 95.1
MS.gene57393.t1 AT5G08790 39.623 159 72 6 5 139 2 160 3.39e-23 93.6
MS.gene57393.t1 AT3G04060 33.838 198 101 8 3 177 13 203 6.77e-23 93.6
MS.gene57393.t1 AT5G18270 37.736 159 76 5 3 138 14 172 7.39e-23 93.2
MS.gene57393.t1 AT3G15510 32.308 195 93 6 14 175 21 209 1.50e-22 92.8
MS.gene57393.t1 AT5G18270 37.107 159 77 5 3 138 14 172 1.77e-22 92.4
MS.gene57393.t1 AT1G56010 35.484 155 76 6 8 138 17 171 3.04e-22 91.7
MS.gene57393.t1 AT4G10350 37.888 161 77 7 2 139 1 161 4.48e-22 91.3
MS.gene57393.t1 AT3G17730 38.065 155 74 6 6 138 2 156 2.08e-21 87.8
MS.gene57393.t1 AT1G33280 37.179 156 78 6 4 139 2 157 2.39e-21 89.0
MS.gene57393.t1 AT5G53950 36.782 174 85 7 10 158 17 190 2.94e-21 89.4
MS.gene57393.t1 AT4G27410 34.234 222 96 11 6 178 10 230 2.97e-21 88.6
MS.gene57393.t1 AT1G79580 36.471 170 81 8 2 144 3 172 3.77e-21 89.4
MS.gene57393.t1 AT1G79580 36.471 170 81 8 2 144 3 172 3.77e-21 89.4
MS.gene57393.t1 AT1G79580 36.471 170 81 8 2 144 3 172 3.77e-21 89.4
MS.gene57393.t1 AT1G79580 36.471 170 81 8 2 144 3 172 3.77e-21 89.4
MS.gene57393.t1 AT1G79580 36.471 170 81 8 2 144 3 172 3.77e-21 89.4
MS.gene57393.t1 AT3G10480 37.419 155 75 7 6 138 23 177 4.23e-21 89.7
MS.gene57393.t1 AT3G10480 37.179 156 75 7 6 138 23 178 5.77e-21 89.4
MS.gene57393.t1 AT3G29035 42.188 128 58 4 8 119 22 149 6.88e-21 87.8
MS.gene57393.t1 AT5G62380 34.591 159 81 7 5 141 2 159 1.05e-20 87.8
MS.gene57393.t1 AT5G62380 34.591 159 81 7 5 141 2 159 1.05e-20 87.8
MS.gene57393.t1 AT3G10490 34.839 155 78 7 7 138 24 178 2.36e-20 85.1
MS.gene57393.t1 AT3G15170 36.601 153 73 5 10 138 20 172 4.21e-20 85.5
MS.gene57393.t1 AT5G17260 35.897 156 77 6 6 138 2 157 8.73e-20 85.9
MS.gene57393.t1 AT1G76420 35.583 163 77 7 1 138 12 171 9.66e-20 84.7
MS.gene57393.t1 AT3G10490 34.839 155 78 7 7 138 24 178 1.33e-19 85.5
MS.gene57393.t1 AT2G18060 33.129 163 88 6 7 148 6 168 1.52e-19 84.7
MS.gene57393.t1 AT2G18060 33.129 163 88 6 7 148 6 168 1.52e-19 84.7
MS.gene57393.t1 AT4G36160 34.118 170 90 7 1 148 5 174 2.22e-19 84.3
MS.gene57393.t1 AT4G36160 34.118 170 90 7 1 148 1 170 2.28e-19 84.3
MS.gene57393.t1 AT4G36160 34.118 170 90 7 1 148 1 170 2.28e-19 84.3
MS.gene57393.t1 AT3G03200 35.714 154 76 6 8 138 4 157 2.42e-19 84.7
MS.gene57393.t1 AT2G43000 31.410 156 86 5 12 146 20 175 4.04e-19 82.4
MS.gene57393.t1 AT1G65910 37.255 153 74 6 8 138 4 156 8.38e-19 83.2
MS.gene57393.t1 AT1G52880 36.250 160 70 6 14 141 21 180 1.27e-18 81.6
MS.gene57393.t1 AT5G04410 35.802 162 76 7 2 138 1 159 2.05e-18 82.0
MS.gene57393.t1 AT1G32770 31.098 164 80 6 9 139 15 178 3.41e-18 80.9
MS.gene57393.t1 AT1G62700 35.484 155 77 7 9 141 6 159 4.25e-18 80.9
MS.gene57393.t1 AT1G62700 35.484 155 77 7 9 141 6 159 4.25e-18 80.9
MS.gene57393.t1 AT1G54330 34.868 152 80 6 8 140 4 155 5.42e-18 79.7
MS.gene57393.t1 AT3G61910 32.530 166 76 7 9 138 10 175 6.52e-18 79.7
MS.gene57393.t1 AT1G12260 34.615 156 75 8 10 141 7 159 1.50e-17 79.0
MS.gene57393.t1 AT5G66300 33.333 159 80 7 7 141 9 165 1.96e-17 78.2
MS.gene57393.t1 AT3G10500 35.152 165 79 7 2 141 1 162 1.99e-17 79.3
MS.gene57393.t1 AT1G12260 34.615 156 75 8 10 141 7 159 2.17e-17 79.0
MS.gene57393.t1 AT3G12910 30.682 176 83 9 5 143 17 190 4.05e-17 77.4
MS.gene57393.t1 AT4G28530 33.333 165 74 6 9 138 9 172 4.25e-17 77.8
MS.gene57393.t1 AT4G35580 34.161 161 80 7 2 138 1 159 5.12e-17 78.2
MS.gene57393.t1 AT4G35580 34.161 161 80 7 2 138 1 159 6.74e-17 77.8
MS.gene57393.t1 AT1G71930 32.903 155 83 6 10 143 9 163 6.76e-17 77.0
MS.gene57393.t1 AT1G71930 32.903 155 83 6 10 143 9 163 6.76e-17 77.0
MS.gene57393.t1 AT4G35580 34.161 161 80 7 2 138 1 159 7.01e-17 77.8
MS.gene57393.t1 AT1G33060 33.333 153 76 7 10 138 24 174 1.16e-16 77.0
MS.gene57393.t1 AT1G33060 33.333 153 76 7 10 138 24 174 1.19e-16 77.0
MS.gene57393.t1 AT3G10480 31.746 189 73 7 6 138 23 211 1.46e-16 76.6
MS.gene57393.t1 AT4G17980 34.356 163 78 7 2 138 1 160 2.10e-16 74.7
MS.gene57393.t1 AT5G39820 31.395 172 71 7 12 138 22 191 2.11e-16 75.9
MS.gene57393.t1 AT4G17980 34.356 163 78 7 2 138 1 160 2.34e-16 75.1
MS.gene57393.t1 AT5G46590 34.161 161 79 7 2 138 1 158 3.04e-16 74.7
MS.gene57393.t1 AT1G26870 33.125 160 76 6 10 138 16 175 3.34e-16 75.5
MS.gene57393.t1 AT2G17040 35.714 154 69 7 12 141 8 155 7.45e-16 73.6
MS.gene57393.t1 AT2G02450 33.557 149 74 6 12 138 53 198 1.03e-15 73.9
MS.gene57393.t1 AT2G02450 33.557 149 74 6 12 138 53 198 1.35e-15 73.9
MS.gene57393.t1 AT1G02230 32.414 145 78 5 11 135 4 148 2.47e-15 72.8
MS.gene57393.t1 AT5G46590 35.507 138 67 6 2 120 1 135 6.44e-15 71.2
MS.gene57393.t1 AT5G50820 34.058 138 73 4 6 128 13 147 1.53e-14 68.6
MS.gene57393.t1 AT5G14000 30.058 173 100 6 8 161 13 183 2.79e-14 68.2
MS.gene57393.t1 AT4G01520 32.468 154 80 6 8 138 2 154 9.47e-14 68.2
MS.gene57393.t1 AT1G02250 31.034 145 80 4 11 135 4 148 1.70e-13 67.8
MS.gene57393.t1 AT3G49530 29.167 168 92 7 10 152 13 178 2.48e-13 67.4
MS.gene57393.t1 AT3G49530 29.167 168 92 7 10 152 13 178 3.94e-13 66.6
MS.gene57393.t1 AT2G33480 39.604 101 42 5 64 145 10 110 1.27e-11 61.2
MS.gene57393.t1 AT1G34180 34.127 126 65 5 12 119 18 143 3.93e-11 61.2
MS.gene57393.t1 AT1G34180 34.127 126 65 5 12 119 18 143 4.85e-11 60.8
MS.gene57393.t1 AT1G32870 33.766 154 80 6 10 141 10 163 5.24e-11 60.8
MS.gene57393.t1 AT1G32870 33.766 154 80 6 10 141 10 163 5.33e-11 60.8
MS.gene57393.t1 AT1G32870 33.766 154 80 6 10 141 46 199 5.97e-11 60.5
MS.gene57393.t1 AT5G61430 38.542 96 38 4 64 138 8 103 6.65e-11 59.7
MS.gene57393.t1 AT2G43000 31.522 92 51 3 67 146 6 97 8.10e-11 58.5
MS.gene57393.t1 AT1G56010 36.957 92 44 4 61 138 13 104 9.93e-11 59.3

Find 47 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAAAACCTATAAATAAAT+TGG 0.146052 4.2:+9038330 MS.gene57393:CDS
TTCATTCCCAATTTATTTAT+AGG 0.164515 4.2:-9038337 None:intergenic
AATGGATATTGGAAGGAAAT+TGG 0.199332 4.2:+9038304 MS.gene57393:CDS
AAGCTAATTATTAACTAATT+AGG 0.204594 4.2:-9038758 None:intergenic
GAAAATAGAAGCACTGAAAA+TGG 0.301344 4.2:+9038286 MS.gene57393:CDS
ATGGATATTGGAAGGAAATT+GGG 0.330470 4.2:+9038305 MS.gene57393:CDS
CATGATCCTTGGGGAATATA+CGG 0.342520 4.2:+9037611 MS.gene57393:CDS
ACTTGTCTCACCATGATCCT+TGG 0.355757 4.2:+9037600 MS.gene57393:CDS
CTTGTCTCACCATGATCCTT+GGG 0.361857 4.2:+9037601 MS.gene57393:CDS
GTGTGAGACAGGATCATAAT+TGG 0.363529 4.2:+9038588 MS.gene57393:intron
GAAGCACTGAAAATGGATAT+TGG 0.365813 4.2:+9038293 MS.gene57393:CDS
TTGATCAAGATCATCATCTA+AGG 0.370976 4.2:-9038726 None:intergenic
TGGTGAGACAAGTGAAGTTC+AGG 0.398752 4.2:-9037590 None:intergenic
AGTACCTTGTATTCAACCTT+GGG 0.405124 4.2:+9038362 MS.gene57393:CDS
GGTATGATGCTAGGATGATA+TGG 0.434256 4.2:-9037569 None:intergenic
GATGAGGATATTGATGATAA+TGG 0.434866 4.2:+9038668 MS.gene57393:CDS
CTTCCCCAAGGTTGAATACA+AGG 0.441845 4.2:-9038366 None:intergenic
ACAAAACCTATAAATAAATT+GGG 0.452946 4.2:+9038331 MS.gene57393:CDS
AGCAGCGTGAAGCTTCCTCC+TGG 0.455992 4.2:+9037482 MS.gene57393:CDS
AATTGAAACAAGTTGGGTAA+TGG 0.457936 4.2:+9038390 MS.gene57393:CDS
CGTATATTCCCCAAGGATCA+TGG 0.467427 4.2:-9037610 None:intergenic
GGGAAGGAATTGAAACAAGT+TGG 0.476317 4.2:+9038383 MS.gene57393:CDS
ATGATATGGTAAAGAAGCCT+TGG 0.489756 4.2:-9037555 None:intergenic
GGAAGGAATTGAAACAAGTT+GGG 0.495348 4.2:+9038384 MS.gene57393:CDS
ATATCATATTTCCTCATCTC+CGG 0.517814 4.2:+9038417 MS.gene57393:CDS
AGGACTAGTTCTTCATCCGT+TGG 0.520052 4.2:-9037518 None:intergenic
AGGATCATAATTGGAGCAGA+TGG 0.520438 4.2:+9038597 MS.gene57393:intron
TAATTATTAACTAATTAGGT+AGG 0.520877 4.2:-9038754 None:intergenic
TGGATATTGGAAGGAAATTG+GGG 0.532640 4.2:+9038306 MS.gene57393:CDS
AAGTACCTTGTATTCAACCT+TGG 0.541631 4.2:+9038361 MS.gene57393:CDS
CTTGTATTCAACCTTGGGGA+AGG 0.542660 4.2:+9038367 MS.gene57393:CDS
GCAGCGTGAAGCTTCCTCCT+GGG 0.544030 4.2:+9037483 MS.gene57393:CDS
TGAAGTTCAGGTATGATGCT+AGG 0.561302 4.2:-9037578 None:intergenic
TTGTTTCAATTCCTTCCCCA+AGG 0.571160 4.2:-9038378 None:intergenic
GGATCATAATTGGAGCAGAT+GGG 0.571776 4.2:+9038598 MS.gene57393:intron
CCTTGGAATAGAGAAAATGA+AGG 0.572117 4.2:-9037538 None:intergenic
CACTGAAAATGGATATTGGA+AGG 0.575605 4.2:+9038297 MS.gene57393:CDS
GAAAAGAAGATGTCACATCA+AGG 0.579347 4.2:+9038638 MS.gene57393:CDS
ACAATCACTTACCGGAGATG+AGG 0.598757 4.2:-9038428 None:intergenic
ACAGGTAAAGCATTGTCAAG+TGG 0.622513 4.2:+9038232 MS.gene57393:intron
GACATACCGTATATTCCCCA+AGG 0.643710 4.2:-9037617 None:intergenic
ACATCAAGGTGCAAGCGATG+AGG 0.654589 4.2:+9038652 MS.gene57393:CDS
GTACCTTGTATTCAACCTTG+GGG 0.661844 4.2:+9038363 MS.gene57393:CDS
TTGTCTCACCATGATCCTTG+GGG 0.667985 4.2:+9037602 MS.gene57393:CDS
ATAATGGAACAGATGTGTCA+TGG 0.669182 4.2:+9038684 MS.gene57393:CDS
GTTGGATCAAAGTAAAACCC+AGG 0.688035 4.2:-9037500 None:intergenic
GGATCAAAGTAAAACCCAGG+AGG 0.734981 4.2:-9037497 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTATTTTATTTTTTACC+CGG - chr4.2:9038096-9038115 None:intergenic 10.0%
!!! TTTTCTTTTAAAATAGAATA+TGG + chr4.2:9037989-9038008 MS.gene57393:intron 10.0%
!! ACAAAACCTATAAATAAATT+GGG + chr4.2:9038331-9038350 MS.gene57393:CDS 15.0%
!! ATTCTAAATACGATTTAAAT+TGG + chr4.2:9037861-9037880 MS.gene57393:intron 15.0%
!! TATAATTAATCTATAAGTGA+TGG - chr4.2:9038052-9038071 None:intergenic 15.0%
!! TTCTAAATACGATTTAAATT+GGG + chr4.2:9037862-9037881 MS.gene57393:intron 15.0%
!!! TAATTTTTTTCCACATTAAT+TGG - chr4.2:9038519-9038538 None:intergenic 15.0%
!! AAATTTATGTAAAACCTATC+TGG + chr4.2:9038141-9038160 MS.gene57393:intron 20.0%
!! ATTAGCAATAACATATGTTA+AGG - chr4.2:9037907-9037926 None:intergenic 20.0%
!! CACAAAACCTATAAATAAAT+TGG + chr4.2:9038330-9038349 MS.gene57393:CDS 20.0%
!! GTGTTAAAGACAAAATAAAT+TGG - chr4.2:9037764-9037783 None:intergenic 20.0%
!! TAAAGACAAAATAAATTGGT+AGG - chr4.2:9037760-9037779 None:intergenic 20.0%
!! TTCATTCCCAATTTATTTAT+AGG - chr4.2:9038340-9038359 None:intergenic 20.0%
!!! ATTTGTTGTGATTTATGAAT+AGG - chr4.2:9038472-9038491 None:intergenic 20.0%
!!! CATAGCATATAATTGAATTT+TGG - chr4.2:9037964-9037983 None:intergenic 20.0%
!!! GATAGGTTTTACATAAATTT+TGG - chr4.2:9038141-9038160 None:intergenic 20.0%
!!! TCAAAGTTTTATCTAAAATC+CGG + chr4.2:9038074-9038093 MS.gene57393:intron 20.0%
! TCTATATATGCATGCATATA+TGG - chr4.2:9037725-9037744 None:intergenic 25.0%
!! ATTAATCTATAAGTGATGGA+TGG - chr4.2:9038048-9038067 None:intergenic 25.0%
!!! CAAAGTTTTATCTAAAATCC+GGG + chr4.2:9038075-9038094 MS.gene57393:intron 25.0%
AACAAAACACAATCACTTAC+CGG - chr4.2:9038439-9038458 None:intergenic 30.0%
AATCGTACTCTTTATAGTTG+TGG + chr4.2:9038166-9038185 MS.gene57393:intron 30.0%
AATGCTTTACCTGTTTACAA+AGG - chr4.2:9038226-9038245 None:intergenic 30.0%
AATGGATATTGGAAGGAAAT+TGG + chr4.2:9038304-9038323 MS.gene57393:CDS 30.0%
ATATACATGCCTTTGTAAAC+AGG + chr4.2:9038214-9038233 MS.gene57393:intron 30.0%
ATATCATATTTCCTCATCTC+CGG + chr4.2:9038417-9038436 MS.gene57393:CDS 30.0%
ATGGATATTGGAAGGAAATT+GGG + chr4.2:9038305-9038324 MS.gene57393:CDS 30.0%
ATTCAATGATCGATTAATCC+CGG + chr4.2:9038012-9038031 MS.gene57393:intron 30.0%
ATTGATCAAGTGACATATAC+TGG + chr4.2:9037687-9037706 MS.gene57393:intron 30.0%
CAAATCATGACCAATTAATG+TGG + chr4.2:9038506-9038525 MS.gene57393:intron 30.0%
TAACATATGTTAAGGTCGAA+AGG - chr4.2:9037899-9037918 None:intergenic 30.0%
TTGATCAAGATCATCATCTA+AGG - chr4.2:9038729-9038748 None:intergenic 30.0%
! AAAGGCATGTATATTTCTTG+GGG - chr4.2:9038208-9038227 None:intergenic 30.0%
! AATTGAAACAAGTTGGGTAA+TGG + chr4.2:9038390-9038409 MS.gene57393:CDS 30.0%
! ACAAAGGCATGTATATTTCT+TGG - chr4.2:9038210-9038229 None:intergenic 30.0%
! CAAAGGCATGTATATTTCTT+GGG - chr4.2:9038209-9038228 None:intergenic 30.0%
! GATGAGGATATTGATGATAA+TGG + chr4.2:9038668-9038687 MS.gene57393:CDS 30.0%
!! GAAAATAGAAGCACTGAAAA+TGG + chr4.2:9038286-9038305 MS.gene57393:CDS 30.0%
!! TATTTTTGATTGTGTGAGAC+AGG + chr4.2:9038577-9038596 MS.gene57393:intron 30.0%
!!! CTTACTTTCTAGCTGAATTT+TGG - chr4.2:9037818-9037837 None:intergenic 30.0%
AAGGCATGTATATTTCTTGG+GGG - chr4.2:9038207-9038226 None:intergenic 35.0%
AAGTACCTTGTATTCAACCT+TGG + chr4.2:9038361-9038380 MS.gene57393:CDS 35.0%
ACGGTATGTCTTATAGTACT+AGG + chr4.2:9037630-9037649 MS.gene57393:intron 35.0%
AGTACCTTGTATTCAACCTT+GGG + chr4.2:9038362-9038381 MS.gene57393:CDS 35.0%
ATGATATGGTAAAGAAGCCT+TGG - chr4.2:9037558-9037577 None:intergenic 35.0%
CACTGAAAATGGATATTGGA+AGG + chr4.2:9038297-9038316 MS.gene57393:CDS 35.0%
CCTTGGAATAGAGAAAATGA+AGG - chr4.2:9037541-9037560 None:intergenic 35.0%
GAAAAGAAGATGTCACATCA+AGG + chr4.2:9038638-9038657 MS.gene57393:CDS 35.0%
GGAAGGAATTGAAACAAGTT+GGG + chr4.2:9038384-9038403 MS.gene57393:CDS 35.0%
TGGATATTGGAAGGAAATTG+GGG + chr4.2:9038306-9038325 MS.gene57393:CDS 35.0%
! ATAATGGAACAGATGTGTCA+TGG + chr4.2:9038684-9038703 MS.gene57393:CDS 35.0%
! CCTTCATTTTCTCTATTCCA+AGG + chr4.2:9037538-9037557 MS.gene57393:CDS 35.0%
! GCTTCTATTTTCCTTCACTT+CGG - chr4.2:9038278-9038297 None:intergenic 35.0%
! TGGGTTTTACTTTGATCCAA+CGG + chr4.2:9037502-9037521 MS.gene57393:CDS 35.0%
!! AAGAGTACGATTTTCCAGAT+AGG - chr4.2:9038158-9038177 None:intergenic 35.0%
!! GAAGCACTGAAAATGGATAT+TGG + chr4.2:9038293-9038312 MS.gene57393:CDS 35.0%
AGGATCATAATTGGAGCAGA+TGG + chr4.2:9038597-9038616 MS.gene57393:intron 40.0%
CATGATCCTTGGGGAATATA+CGG + chr4.2:9037611-9037630 MS.gene57393:CDS 40.0%
GGATCATAATTGGAGCAGAT+GGG + chr4.2:9038598-9038617 MS.gene57393:intron 40.0%
GGGAAGGAATTGAAACAAGT+TGG + chr4.2:9038383-9038402 MS.gene57393:CDS 40.0%
GGTATGATGCTAGGATGATA+TGG - chr4.2:9037572-9037591 None:intergenic 40.0%
GTACCTTGTATTCAACCTTG+GGG + chr4.2:9038363-9038382 MS.gene57393:CDS 40.0%
GTGTGAGACAGGATCATAAT+TGG + chr4.2:9038588-9038607 MS.gene57393:intron 40.0%
GTTGGATCAAAGTAAAACCC+AGG - chr4.2:9037503-9037522 None:intergenic 40.0%
TGAAGTTCAGGTATGATGCT+AGG - chr4.2:9037581-9037600 None:intergenic 40.0%
TTGTTTCAATTCCTTCCCCA+AGG - chr4.2:9038381-9038400 None:intergenic 40.0%
!! ACAGGTAAAGCATTGTCAAG+TGG + chr4.2:9038232-9038251 MS.gene57393:intron 40.0%
!! TAAGTGATGGATGGTGAGAT+TGG - chr4.2:9038039-9038058 None:intergenic 40.0%
!!! CTACTTTTTCACCGAAGTGA+AGG + chr4.2:9038264-9038283 MS.gene57393:CDS 40.0%
ACAATCACTTACCGGAGATG+AGG - chr4.2:9038431-9038450 None:intergenic 45.0%
ACTTGTCTCACCATGATCCT+TGG + chr4.2:9037600-9037619 MS.gene57393:CDS 45.0%
CGTATATTCCCCAAGGATCA+TGG - chr4.2:9037613-9037632 None:intergenic 45.0%
CTTCCCCAAGGTTGAATACA+AGG - chr4.2:9038369-9038388 None:intergenic 45.0%
CTTGTATTCAACCTTGGGGA+AGG + chr4.2:9038367-9038386 MS.gene57393:CDS 45.0%
CTTGTCTCACCATGATCCTT+GGG + chr4.2:9037601-9037620 MS.gene57393:CDS 45.0%
GACATACCGTATATTCCCCA+AGG - chr4.2:9037620-9037639 None:intergenic 45.0%
GGATCAAAGTAAAACCCAGG+AGG - chr4.2:9037500-9037519 None:intergenic 45.0%
TGGTGAGACAAGTGAAGTTC+AGG - chr4.2:9037593-9037612 None:intergenic 45.0%
TTGTCTCACCATGATCCTTG+GGG + chr4.2:9037602-9037621 MS.gene57393:CDS 45.0%
!! AGGACTAGTTCTTCATCCGT+TGG - chr4.2:9037521-9037540 None:intergenic 45.0%
ACATCAAGGTGCAAGCGATG+AGG + chr4.2:9038652-9038671 MS.gene57393:CDS 50.0%
! ATGGATGGTGAGATTGGTCC+GGG - chr4.2:9038033-9038052 None:intergenic 50.0%
! GATGGATGGTGAGATTGGTC+CGG - chr4.2:9038034-9038053 None:intergenic 50.0%
AGCAGCGTGAAGCTTCCTCC+TGG + chr4.2:9037482-9037501 MS.gene57393:CDS 60.0%
GCAGCGTGAAGCTTCCTCCT+GGG + chr4.2:9037483-9037502 MS.gene57393:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 9037467 9038766 9037467 ID=MS.gene57393
chr4.2 mRNA 9037467 9038766 9037467 ID=MS.gene57393.t1;Parent=MS.gene57393
chr4.2 exon 9037467 9037632 9037467 ID=MS.gene57393.t1.exon1;Parent=MS.gene57393.t1
chr4.2 CDS 9037467 9037632 9037467 ID=cds.MS.gene57393.t1;Parent=MS.gene57393.t1
chr4.2 exon 9038236 9038438 9038236 ID=MS.gene57393.t1.exon2;Parent=MS.gene57393.t1
chr4.2 CDS 9038236 9038438 9038236 ID=cds.MS.gene57393.t1;Parent=MS.gene57393.t1
chr4.2 exon 9038599 9038766 9038599 ID=MS.gene57393.t1.exon3;Parent=MS.gene57393.t1
chr4.2 CDS 9038599 9038766 9038599 ID=cds.MS.gene57393.t1;Parent=MS.gene57393.t1
Gene Sequence

>MS.gene57393

ATGATGGGGAGTGAAAGCAGCGTGAAGCTTCCTCCTGGGTTTTACTTTGATCCAACGGATGAAGAACTAGTCCTTCATTTTCTCTATTCCAAGGCTTCTTTACCATATCATCCTAGCATCATACCTGAACTTCACTTGTCTCACCATGATCCTTGGGGAATATACGGTATGTCTTATAGTACTAGGTATATATATTTCATATAATATTTTCAATTTCAATATTGATCAAGTGACATATACTGGATATTTAGTTGACCATATATGCATGCATATATAGATAAAACTTCATTCCTACCAATTTATTTTGTCTTTAACACTTTGATTCTCATTAAAATTAGCTCTCATAGTCCAAAATTCAGCTAGAAAGTAAGTAAGTAAAATCTAACAAAAAGATATTCTAAATACGATTTAAATTGGGATACTCTCAATCCTTTCGACCTTAACATATGTTATTGCTAATTAAAAATATAGTTTGTGATATATGTAATCTTCAGCCAAAATTCAATTATATGCTATGAGATTTTTTCTTTTAAAATAGAATATGGATTCAATGATCGATTAATCCCGGACCAATCTCACCATCCATCACTTATAGATTAATTATATATCAAAGTTTTATCTAAAATCCGGGTAAAAAATAAAATAAATATACGTTATTTGTAGAAAATTATCCAAAATTTATGTAAAACCTATCTGGAAAATCGTACTCTTTATAGTTGTGGATGTGTATACTAACACCCCCAAGAAATATACATGCCTTTGTAAACAGGTAAAGCATTGTCAAGTGGAAATGAGCACTACTTTTTCACCGAAGTGAAGGAAAATAGAAGCACTGAAAATGGATATTGGAAGGAAATTGGGGTCACAAAACCTATAAATAAATTGGGAATGAAGAAGTACCTTGTATTCAACCTTGGGGAAGGAATTGAAACAAGTTGGGTAATGGAAGAATATCATATTTCCTCATCTCCGGTAAGTGATTGTGTTTTGTTTTGTTAACTACCTATTCATAAATCACAACAAATTACTTACTAAGAAACAAATCATGACCAATTAATGTGGAAAAAAATTAACATGAAGAACATAAAATTAAATTGATTTGTATCAATTTATTTTTGATTGTGTGAGACAGGATCATAATTGGAGCAGATGGGTTTTATGCAAAGTTTATGAAAAGAAGATGTCACATCAAGGTGCAAGCGATGAGGATATTGATGATAATGGAACAGATGTGTCATGGCTAGATGAAGTGTTTATGTCCTTAGATGATGATCTTGATCAAACAAGCCTACCTAATTAG
Protein sequence

>MS.gene57393.t1

MMGSESSVKLPPGFYFDPTDEELVLHFLYSKASLPYHPSIIPELHLSHHDPWGIYGKALSSGNEHYFFTEVKENRSTENGYWKEIGVTKPINKLGMKKYLVFNLGEGIETSWVMEEYHISSSPDHNWSRWVLCKVYEKKMSHQGASDEDIDDNGTDVSWLDEVFMSLDDDLDQTSLPN