AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57419

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57419.t1 MTR_5g014640 94.408 304 15 1 19 322 1 302 0.0 566
MS.gene57419.t1 MTR_4g097940 46.707 334 118 15 19 322 1 304 1.52e-73 230
MS.gene57419.t1 MTR_4g097920 43.713 334 128 15 19 322 1 304 1.23e-67 215
MS.gene57419.t1 MTR_4g097920 43.260 319 122 14 33 322 1 289 1.39e-62 201
MS.gene57419.t1 MTR_2g010450 35.474 327 185 11 20 322 5 329 6.08e-48 164
MS.gene57419.t1 MTR_5g014600 42.396 217 104 6 122 322 102 313 4.73e-45 156
MS.gene57419.t1 MTR_5g014520 39.344 244 128 4 81 322 83 308 2.03e-44 155
MS.gene57419.t1 MTR_4g092700 34.323 303 169 10 33 322 24 309 2.48e-44 154
MS.gene57419.t1 MTR_2g104550 37.553 237 122 6 93 322 73 290 2.05e-40 144
MS.gene57419.t1 MTR_0250s0040 45.806 155 83 1 169 322 85 239 8.68e-40 140
MS.gene57419.t1 MTR_4g009540 40.488 205 103 4 134 322 108 309 2.49e-39 141
MS.gene57419.t1 MTR_4g009540 40.488 205 103 4 134 322 108 309 4.93e-39 140
MS.gene57419.t1 MTR_4g067010 44.375 160 83 2 165 322 140 295 6.82e-39 140
MS.gene57419.t1 MTR_4g009110 36.842 247 125 7 86 322 103 328 3.60e-38 139
MS.gene57419.t1 MTR_0011s0260 46.104 154 82 1 170 322 181 334 4.69e-38 139
MS.gene57419.t1 MTR_0011s0210 46.452 155 80 2 170 322 181 334 4.84e-38 139
MS.gene57419.t1 MTR_6g047570 43.949 157 87 1 167 322 154 310 4.02e-37 135
MS.gene57419.t1 MTR_0246s0020 43.258 178 85 6 156 322 181 353 1.61e-36 135
MS.gene57419.t1 MTR_0246s0020 43.258 178 85 6 156 322 181 353 1.75e-36 135
MS.gene57419.t1 MTR_2g104560 42.073 164 86 5 163 322 45 203 1.91e-36 131
MS.gene57419.t1 MTR_5g014560 41.011 178 100 3 149 322 106 282 2.08e-36 133
MS.gene57419.t1 MTR_4g066460 43.784 185 87 4 144 322 130 303 4.63e-36 132
MS.gene57419.t1 MTR_8g069740 34.914 232 130 6 97 322 49 265 3.71e-35 129
MS.gene57419.t1 MTR_8g009020 40.476 168 88 3 165 322 136 301 8.21e-35 129
MS.gene57419.t1 MTR_2g104490 35.498 231 123 6 105 322 69 286 1.23e-34 128
MS.gene57419.t1 MTR_2g104490 35.498 231 123 6 105 322 69 286 2.26e-34 128
MS.gene57419.t1 MTR_4g066380 41.111 180 98 4 144 322 128 300 3.05e-33 125
MS.gene57419.t1 MTR_7g080780 41.772 158 84 1 165 322 145 294 3.56e-33 125
MS.gene57419.t1 MTR_7g080780 41.772 158 84 1 165 322 158 307 3.78e-33 125
MS.gene57419.t1 MTR_4g097950 38.503 187 97 3 151 322 45 228 2.15e-31 118
MS.gene57419.t1 MTR_0246s0050 36.686 169 98 3 156 322 134 295 6.37e-31 119
MS.gene57419.t1 MTR_4g098035 36.686 169 98 3 156 322 155 316 1.15e-30 119
MS.gene57419.t1 MTR_2g104650 42.331 163 87 3 165 322 87 247 1.08e-29 114
MS.gene57419.t1 MTR_2g104590 35.838 173 84 4 155 322 52 202 4.91e-29 111
MS.gene57419.t1 MTR_8g009010 37.736 159 63 2 165 322 127 250 1.02e-27 110
MS.gene57419.t1 MTR_2g104530 35.176 199 106 4 129 322 72 252 1.05e-27 109
MS.gene57419.t1 MTR_2g104500 38.961 154 84 3 172 322 104 250 1.39e-27 108
MS.gene57419.t1 MTR_6g047550 63.636 66 24 0 167 232 168 233 2.56e-21 92.0
MS.gene57419.t1 MTR_8g067280 36.196 163 70 5 165 302 451 604 2.49e-20 92.0
MS.gene57419.t1 MTR_4g009420 38.281 128 62 1 195 322 65 175 1.39e-19 85.5
MS.gene57419.t1 MTR_5g014550 36.585 123 75 2 202 322 54 175 1.44e-19 85.5
MS.gene57419.t1 MTR_4g009420 38.281 128 62 1 195 322 65 175 1.86e-19 85.1
MS.gene57419.t1 MTR_7g451650 35.938 128 73 1 195 322 57 175 9.79e-19 82.8
MS.gene57419.t1 MTR_7g451650 35.938 128 73 1 195 322 57 175 4.14e-18 81.6
MS.gene57419.t1 MTR_1g072320 34.532 139 82 2 173 302 471 609 4.61e-18 85.1
MS.gene57419.t1 MTR_5g030430 35.443 158 86 3 165 306 482 639 9.66e-18 84.3
MS.gene57419.t1 MTR_7g117670 37.705 122 51 3 173 284 403 509 1.26e-17 84.0
MS.gene57419.t1 MTR_4g009510 33.333 141 83 3 183 322 53 183 4.75e-17 78.6
MS.gene57419.t1 MTR_8g027495 41.071 112 53 3 173 281 459 560 1.08e-16 81.3
MS.gene57419.t1 MTR_7g050350 33.607 122 72 1 201 322 1 113 1.17e-15 73.2
MS.gene57419.t1 MTR_7g451650 33.607 122 72 1 201 322 1 113 1.17e-15 73.2
MS.gene57419.t1 MTR_7g083900 32.500 160 93 3 172 317 286 444 1.45e-15 77.4
MS.gene57419.t1 MTR_7g083900 32.500 160 93 3 172 317 286 444 1.48e-15 77.4
MS.gene57419.t1 MTR_8g024770 36.029 136 71 4 173 296 280 411 1.89e-15 77.0
MS.gene57419.t1 MTR_8g024790 32.117 137 91 1 173 307 292 428 2.91e-15 76.6
MS.gene57419.t1 MTR_8g064700 25.146 171 122 2 156 320 228 398 6.06e-15 75.5
MS.gene57419.t1 MTR_3g030700 30.052 193 111 7 148 322 166 352 3.11e-14 73.2
MS.gene57419.t1 MTR_1g112680 32.836 134 81 3 104 236 256 381 3.24e-13 70.5
MS.gene57419.t1 MTR_7g096350 34.146 123 54 3 163 281 342 441 4.55e-13 70.1
MS.gene57419.t1 MTR_3g498695 29.560 159 97 4 172 317 298 454 7.11e-13 69.3
MS.gene57419.t1 MTR_3g498695 29.560 159 97 4 172 317 298 454 7.45e-13 69.3
MS.gene57419.t1 MTR_4g057270 31.206 141 82 4 174 305 151 285 1.45e-12 67.8
MS.gene57419.t1 MTR_8g098275 27.647 170 87 4 169 305 398 564 2.37e-12 68.2
MS.gene57419.t1 MTR_8g098275 27.647 170 87 4 169 305 305 471 2.46e-12 67.8
MS.gene57419.t1 MTR_8g098275 28.313 166 83 4 173 305 245 407 2.85e-12 67.4
MS.gene57419.t1 MTR_2g084280 30.714 140 84 4 174 307 135 267 1.66e-11 64.7
MS.gene57419.t1 MTR_5g030770 28.112 249 146 8 86 310 47 286 3.23e-11 63.9
MS.gene57419.t1 MTR_2g038040 23.656 186 127 4 150 320 29 214 5.69e-11 62.0
MS.gene57419.t1 MTR_3g104100 28.395 162 91 5 174 317 197 351 8.15e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57419.t1 AT4G37850 36.050 319 173 13 20 322 1 304 1.57e-45 157
MS.gene57419.t1 AT4G37850 36.050 319 173 13 20 322 1 304 1.57e-45 157
MS.gene57419.t1 AT2G22760 46.626 163 83 1 164 322 111 273 2.51e-44 153
MS.gene57419.t1 AT2G22760 46.626 163 83 1 164 322 111 273 3.90e-44 153
MS.gene57419.t1 AT2G22750 34.018 341 151 14 20 322 5 309 5.26e-42 148
MS.gene57419.t1 AT2G22750 34.018 341 151 14 20 322 1 305 5.57e-42 148
MS.gene57419.t1 AT2G22750 34.018 341 151 14 20 322 5 309 5.98e-42 148
MS.gene57419.t1 AT2G22750 46.286 175 67 4 164 322 118 281 1.90e-39 141
MS.gene57419.t1 AT2G22750 46.286 175 67 4 164 322 118 281 2.11e-39 141
MS.gene57419.t1 AT2G22770 41.361 191 94 3 150 322 108 298 1.05e-35 131
MS.gene57419.t1 AT4G17880 30.556 216 116 6 109 307 354 552 2.71e-20 92.0
MS.gene57419.t1 AT5G46760 34.356 163 80 4 163 307 402 555 3.10e-19 88.6
MS.gene57419.t1 AT1G32640 35.099 151 92 3 163 307 439 589 4.02e-17 82.4
MS.gene57419.t1 AT1G01260 35.099 151 64 4 168 308 429 555 4.03e-16 79.3
MS.gene57419.t1 AT1G01260 35.099 151 64 4 168 308 429 555 4.03e-16 79.3
MS.gene57419.t1 AT1G01260 35.099 151 64 4 168 308 429 555 4.03e-16 79.3
MS.gene57419.t1 AT2G46510 31.250 144 78 3 163 302 382 508 4.39e-16 79.3
MS.gene57419.t1 AT5G57150 26.344 186 117 3 150 320 32 212 1.57e-15 74.7
MS.gene57419.t1 AT5G57150 26.344 186 117 3 150 320 33 213 2.41e-15 74.3
MS.gene57419.t1 AT5G57150 26.344 186 117 3 150 320 33 213 2.64e-15 74.3
MS.gene57419.t1 AT5G57150 26.344 186 117 3 150 320 33 213 3.28e-15 74.7
MS.gene57419.t1 AT5G57150 26.344 186 117 3 150 320 32 212 4.52e-15 73.9
MS.gene57419.t1 AT1G12860 31.373 153 98 3 172 317 267 419 7.51e-15 75.1
MS.gene57419.t1 AT1G12860 31.373 153 98 3 172 317 267 419 8.53e-15 75.1
MS.gene57419.t1 AT4G09820 34.884 129 63 2 173 300 364 472 1.22e-14 74.7
MS.gene57419.t1 AT2G16910 28.070 171 97 5 173 317 315 485 6.61e-14 72.8
MS.gene57419.t1 AT4G29930 30.137 146 89 3 172 314 54 189 1.56e-13 69.3
MS.gene57419.t1 AT4G29930 30.345 145 88 3 173 314 55 189 2.19e-13 68.9
MS.gene57419.t1 AT4G29930 30.822 146 89 3 173 314 55 192 2.45e-13 68.9
MS.gene57419.t1 AT1G63650 29.480 173 88 6 173 319 406 570 6.65e-13 69.7
MS.gene57419.t1 AT1G63650 29.480 173 88 6 173 319 406 570 6.65e-13 69.7
MS.gene57419.t1 AT1G63650 29.480 173 88 6 173 319 406 570 6.65e-13 69.7
MS.gene57419.t1 AT3G26744 30.573 157 98 3 172 317 307 463 3.32e-12 67.4
MS.gene57419.t1 AT3G26744 30.573 157 98 3 172 317 307 463 4.05e-12 67.0
MS.gene57419.t1 AT3G26744 30.573 157 98 3 172 317 307 463 4.05e-12 67.0
MS.gene57419.t1 AT3G26744 30.573 157 98 3 172 317 307 463 4.05e-12 67.0
MS.gene57419.t1 AT4G16430 45.333 75 37 1 163 233 307 381 4.50e-12 67.0
MS.gene57419.t1 AT4G00870 29.927 137 92 1 173 305 250 386 5.26e-12 66.6
MS.gene57419.t1 AT4G29930 26.667 165 99 3 172 314 54 218 1.30e-11 64.3
MS.gene57419.t1 AT4G29930 26.667 165 99 3 172 314 54 218 1.30e-11 64.3
MS.gene57419.t1 AT4G29930 26.829 164 98 3 173 314 55 218 1.81e-11 63.9
MS.gene57419.t1 AT5G65640 31.293 147 89 4 168 310 174 312 7.25e-11 62.8
MS.gene57419.t1 AT5G41315 29.143 175 91 7 173 319 442 611 7.73e-11 63.5
MS.gene57419.t1 AT5G41315 29.143 175 91 7 173 319 447 616 7.90e-11 63.5
MS.gene57419.t1 AT5G41315 29.143 175 91 7 173 319 450 619 8.51e-11 63.2

Find 58 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATACTCTTACTTCGATTTC+AGG 0.236620 5.1:-7476373 None:intergenic
GTTTCAACTGGGAAACAATT+TGG 0.250112 5.1:-7475158 None:intergenic
TACTTTGGACAGTGAGATTA+AGG 0.260165 5.1:-7476474 None:intergenic
TTCAGGAAGTGGTTGGTTAT+TGG 0.276191 5.1:-7476356 None:intergenic
GCTCTTTCAGCCATTGTTCC+TGG 0.288584 5.1:+7475527 MS.gene57419:CDS
CCAAAGTTGTCGAATAATAT+TGG 0.304765 5.1:-7475107 None:intergenic
TCGAGCTTGAGCAGTGTTCC+TGG 0.329696 5.1:-7475450 None:intergenic
CGATTTGATTAGATGTGGTA+TGG 0.375743 5.1:-7474750 None:intergenic
AATGAATTGAGGTGATGAAT+AGG 0.381227 5.1:-7475240 None:intergenic
CATTGTTCCTGGCCTCAAAA+AGG 0.384959 5.1:+7475538 MS.gene57419:CDS
TCATCAAGATGAGTTTCAAC+TGG 0.389714 5.1:-7475170 None:intergenic
CTTCCAGCTTTGGTTTCTCA+AGG 0.393979 5.1:+7475374 MS.gene57419:CDS
TAAAATTGCTTAGAAGCAAT+TGG 0.399867 5.1:-7475287 None:intergenic
GTTGTTTCCAAAGGGCAAAA+AGG 0.400410 5.1:-7476500 None:intergenic
CCAATATTATTCGACAACTT+TGG 0.407380 5.1:+7475107 MS.gene57419:CDS
AGTAGTATGCTTACAAGTTC+AGG 0.414356 5.1:-7474826 None:intergenic
AGAAGCAATTGGTGTTGAAC+TGG 0.425456 5.1:-7475276 None:intergenic
ATGGACAAAGCTTCTGTGTT+GGG 0.439391 5.1:+7476184 MS.gene57419:CDS
CAAGAGAATCCATCGGGTAT+TGG 0.448559 5.1:-7475079 None:intergenic
TGAAAGAGCGATGAAACTTC+GGG 0.456547 5.1:-7475513 None:intergenic
GGACGTGCCGCTACGGTGCT+TGG 0.467945 5.1:+7476439 MS.gene57419:CDS
ATCAACAATGTTGTTTCCAA+AGG 0.469768 5.1:-7476509 None:intergenic
TCAACAATGTTGTTTCCAAA+GGG 0.475298 5.1:-7476508 None:intergenic
TGATGTAACCATTGTTGCTC+AGG 0.479291 5.1:+7476528 MS.gene57419:CDS
CTGAAAGAGCGATGAAACTT+CGG 0.488970 5.1:-7475514 None:intergenic
TGAGGTGATGAATAGGAAGA+AGG 0.490585 5.1:-7475233 None:intergenic
CTTCGATTTCAGGAAGTGGT+TGG 0.508259 5.1:-7476363 None:intergenic
TAAAACCTAAAGTCGAGAAA+AGG 0.508413 5.1:+7475330 MS.gene57419:CDS
TGTTCCTGGTCTGATTCACT+TGG 0.510038 5.1:-7475436 None:intergenic
GAGGTGATGAATAGGAAGAA+GGG 0.522927 5.1:-7475232 None:intergenic
CTTACTTCGATTTCAGGAAG+TGG 0.529255 5.1:-7476367 None:intergenic
GATTTGATTAGATGTGGTAT+GGG 0.531705 5.1:-7474749 None:intergenic
TTTATTTACCTGAGCAACAA+TGG 0.540344 5.1:-7476536 None:intergenic
GAACCTTGAGAAACCAAAGC+TGG 0.540922 5.1:-7475377 None:intergenic
ACAAGTTCAGGCACATAGGT+GGG 0.548457 5.1:-7474814 None:intergenic
ACAAGCACGTTTGCAGACAC+TGG 0.553923 5.1:+7476234 MS.gene57419:CDS
AACTTCGATTTGATTAGATG+TGG 0.560569 5.1:-7474755 None:intergenic
ACACAGTGGACGTGCCGCTA+CGG 0.562208 5.1:+7476432 MS.gene57419:CDS
CAAGTTCAGGCACATAGGTG+GGG 0.574321 5.1:-7474813 None:intergenic
CTTCTTTAACCAATACCCGA+TGG 0.574424 5.1:+7475070 MS.gene57419:CDS
TGTTCAAAAGAAATGAATTG+AGG 0.577900 5.1:-7475251 None:intergenic
AACTGGGAAACAATTTGGTG+TGG 0.582222 5.1:-7475153 None:intergenic
TCAAGCTCGAGAACATGTAA+TGG 0.585662 5.1:+7475463 MS.gene57419:CDS
TACAAGTTCAGGCACATAGG+TGG 0.586428 5.1:-7474815 None:intergenic
GAGATGCAAGAGAATCCATC+GGG 0.590902 5.1:-7475085 None:intergenic
GCTTACAAGTTCAGGCACAT+AGG 0.592149 5.1:-7474818 None:intergenic
CTCATCTTGATGATCAAACA+AGG 0.597867 5.1:+7475180 MS.gene57419:CDS
CATCAAGATGAGTTTCAACT+GGG 0.598938 5.1:-7475169 None:intergenic
GATGGACAAAGCTTCTGTGT+TGG 0.607222 5.1:+7476183 MS.gene57419:intron
AGATCTCTTCACTTGAACTG+TGG 0.609751 5.1:-7476290 None:intergenic
GGAGATGCAAGAGAATCCAT+CGG 0.627162 5.1:-7475086 None:intergenic
AAGTTCAGGCACATAGGTGG+GGG 0.650925 5.1:-7474812 None:intergenic
AATGGCCGAAAGAAAACGAA+GGG 0.657033 5.1:+7475481 MS.gene57419:CDS
TAATTGGCCAAGCACCGTAG+CGG 0.678187 5.1:-7476446 None:intergenic
AGCACGTTTGCAGACACTGG+AGG 0.686426 5.1:+7476237 MS.gene57419:CDS
ATAACCAAGTGAATCAGACC+AGG 0.687464 5.1:+7475432 MS.gene57419:CDS
TAATGGCCGAAAGAAAACGA+AGG 0.695210 5.1:+7475480 MS.gene57419:CDS
ATTCAATGTGACAAACACAG+TGG 0.762327 5.1:+7476418 MS.gene57419:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATGTATTCAATATTA+TGG + chr5.1:7476152-7476171 MS.gene57419:intron 10.0%
!!! TCAATAATTTATTTTTCTTT+TGG + chr5.1:7475736-7475755 MS.gene57419:intron 10.0%
!! TACAATATAAATATTGCAAT+TGG - chr5.1:7476115-7476134 None:intergenic 15.0%
!!! AAGTTTATGAATTTTAAGAT+AGG - chr5.1:7474859-7474878 None:intergenic 15.0%
!!! TTGTTTTTATTTCATTTTCT+AGG + chr5.1:7475938-7475957 MS.gene57419:intron 15.0%
!! ACATTAATGATCCATATTAA+AGG + chr5.1:7475646-7475665 MS.gene57419:intron 20.0%
!!! TATTTTCTTATGATCAGATT+AGG - chr5.1:7475801-7475820 None:intergenic 20.0%
!!! TCTTTAATTACTTTTGAACA+GGG + chr5.1:7475032-7475051 MS.gene57419:intron 20.0%
! ATTATAACATGTTTGTGAGT+TGG - chr5.1:7475577-7475596 None:intergenic 25.0%
! GTAGTATGTTGATATAGAAA+TGG - chr5.1:7475615-7475634 None:intergenic 25.0%
! TAAAATTGCTTAGAAGCAAT+TGG - chr5.1:7475290-7475309 None:intergenic 25.0%
! TATTGACGAAAAACATGAAA+TGG - chr5.1:7475722-7475741 None:intergenic 25.0%
! TGATATAGAAATGGTTTGAA+TGG - chr5.1:7475606-7475625 None:intergenic 25.0%
! TGTTCAAAAGAAATGAATTG+AGG - chr5.1:7475254-7475273 None:intergenic 25.0%
! TTGGTAAGTTCAACTTAATT+AGG - chr5.1:7476096-7476115 None:intergenic 25.0%
!! TCTAATCAAATCGAAGTTTT+AGG + chr5.1:7474760-7474779 MS.gene57419:CDS 25.0%
!! TGAGATTAAGGTTTTCTAAT+TGG - chr5.1:7476465-7476484 None:intergenic 25.0%
!! TTAGTCTTCGAGAAAAAAAT+AGG + chr5.1:7475831-7475850 MS.gene57419:intron 25.0%
!!! CTCTTTAATTACTTTTGAAC+AGG + chr5.1:7475031-7475050 MS.gene57419:intron 25.0%
AAATGGTCACTAATATTGCT+AGG - chr5.1:7475705-7475724 None:intergenic 30.0%
AAGAGTAGACATCAAAAAGT+TGG + chr5.1:7475877-7475896 MS.gene57419:intron 30.0%
AATATTATGGTATGTGCAGA+TGG + chr5.1:7476165-7476184 MS.gene57419:intron 30.0%
AATGAATTGAGGTGATGAAT+AGG - chr5.1:7475243-7475262 None:intergenic 30.0%
AATGGTCACTAATATTGCTA+GGG - chr5.1:7475704-7475723 None:intergenic 30.0%
AGAGTAGACATCAAAAAGTT+GGG + chr5.1:7475878-7475897 MS.gene57419:intron 30.0%
AGTAATGTTCTACTTACACT+AGG + chr5.1:7476009-7476028 MS.gene57419:intron 30.0%
ATCCATATTAAAGGCTGAAA+TGG + chr5.1:7475655-7475674 MS.gene57419:intron 30.0%
CCAAAGTTGTCGAATAATAT+TGG - chr5.1:7475110-7475129 None:intergenic 30.0%
CCAATATTATTCGACAACTT+TGG + chr5.1:7475107-7475126 MS.gene57419:CDS 30.0%
GATTTGATTAGATGTGGTAT+GGG - chr5.1:7474752-7474771 None:intergenic 30.0%
TAAAACCTAAAGTCGAGAAA+AGG + chr5.1:7475330-7475349 MS.gene57419:CDS 30.0%
! AACTTCGATTTGATTAGATG+TGG - chr5.1:7474758-7474777 None:intergenic 30.0%
! GATAAGATCATACCTTTTTG+AGG - chr5.1:7475553-7475572 None:intergenic 30.0%
! GTTTTACATCGGAATAATCA+AGG - chr5.1:7475316-7475335 None:intergenic 30.0%
!! AATTACTTTTGAACAGGGAA+TGG + chr5.1:7475037-7475056 MS.gene57419:intron 30.0%
!! ATCAACAATGTTGTTTCCAA+AGG - chr5.1:7476512-7476531 None:intergenic 30.0%
!! TCAACAATGTTGTTTCCAAA+GGG - chr5.1:7476511-7476530 None:intergenic 30.0%
!! TTATTTTTCTGGTCGAACTT+TGG + chr5.1:7474964-7474983 MS.gene57419:intron 30.0%
!!! TATCGATGTTTTGATCTTCT+TGG - chr5.1:7475210-7475229 None:intergenic 30.0%
!!! TCATTTTCTAGGATCAAATC+AGG + chr5.1:7475949-7475968 MS.gene57419:intron 30.0%
!!! TTGACCAAGCTTTATTTTTC+TGG + chr5.1:7474953-7474972 MS.gene57419:intron 30.0%
AGTAGTATGCTTACAAGTTC+AGG - chr5.1:7474829-7474848 None:intergenic 35.0%
ATTCAATGTGACAAACACAG+TGG + chr5.1:7476418-7476437 MS.gene57419:CDS 35.0%
CACCATTTCAGCCTTTAATA+TGG - chr5.1:7475660-7475679 None:intergenic 35.0%
CATCAAGATGAGTTTCAACT+GGG - chr5.1:7475172-7475191 None:intergenic 35.0%
CGATTTGATTAGATGTGGTA+TGG - chr5.1:7474753-7474772 None:intergenic 35.0%
GAGTAGACATCAAAAAGTTG+GGG + chr5.1:7475879-7475898 MS.gene57419:intron 35.0%
GATACTCTTACTTCGATTTC+AGG - chr5.1:7476376-7476395 None:intergenic 35.0%
GCTGAAGACAACAAAAAAAC+TGG + chr5.1:7476265-7476284 MS.gene57419:CDS 35.0%
GTTTCAACTGGGAAACAATT+TGG - chr5.1:7475161-7475180 None:intergenic 35.0%
TACTTTGGACAGTGAGATTA+AGG - chr5.1:7476477-7476496 None:intergenic 35.0%
TCATCAAGATGAGTTTCAAC+TGG - chr5.1:7475173-7475192 None:intergenic 35.0%
TCGACCAGAAAAATAAAGCT+TGG - chr5.1:7474960-7474979 None:intergenic 35.0%
! CAAAGATTTTCTTCCAGCTT+TGG + chr5.1:7475364-7475383 MS.gene57419:CDS 35.0%
! CTCATCTTGATGATCAAACA+AGG + chr5.1:7475180-7475199 MS.gene57419:CDS 35.0%
! CTTTTCCCTTCGTTTTCTTT+CGG - chr5.1:7475489-7475508 None:intergenic 35.0%
! TTACACCTTTTCTCGACTTT+AGG - chr5.1:7475338-7475357 None:intergenic 35.0%
!! CTCGACTTTAGGTTTTACAT+CGG - chr5.1:7475327-7475346 None:intergenic 35.0%
!!! GATGTTTTGATCTTCTTGGT+TGG - chr5.1:7475206-7475225 None:intergenic 35.0%
AATGGCCGAAAGAAAACGAA+GGG + chr5.1:7475481-7475500 MS.gene57419:CDS 40.0%
AGATCTCTTCACTTGAACTG+TGG - chr5.1:7476293-7476312 None:intergenic 40.0%
ATAACCAAGTGAATCAGACC+AGG + chr5.1:7475432-7475451 MS.gene57419:CDS 40.0%
ATGGACAAAGCTTCTGTGTT+GGG + chr5.1:7476184-7476203 MS.gene57419:CDS 40.0%
CTGAAAGAGCGATGAAACTT+CGG - chr5.1:7475517-7475536 None:intergenic 40.0%
CTTACTTCGATTTCAGGAAG+TGG - chr5.1:7476370-7476389 None:intergenic 40.0%
CTTCTTTAACCAATACCCGA+TGG + chr5.1:7475070-7475089 MS.gene57419:CDS 40.0%
GAGGTGATGAATAGGAAGAA+GGG - chr5.1:7475235-7475254 None:intergenic 40.0%
TAATGGCCGAAAGAAAACGA+AGG + chr5.1:7475480-7475499 MS.gene57419:CDS 40.0%
TCAAGCTCGAGAACATGTAA+TGG + chr5.1:7475463-7475482 MS.gene57419:CDS 40.0%
TGAAAGAGCGATGAAACTTC+GGG - chr5.1:7475516-7475535 None:intergenic 40.0%
TGAGGTGATGAATAGGAAGA+AGG - chr5.1:7475236-7475255 None:intergenic 40.0%
! AACTGGGAAACAATTTGGTG+TGG - chr5.1:7475156-7475175 None:intergenic 40.0%
! AGTAGTACCTTTTTGCCCTT+TGG + chr5.1:7476493-7476512 MS.gene57419:CDS 40.0%
! GTTGTTTCCAAAGGGCAAAA+AGG - chr5.1:7476503-7476522 None:intergenic 40.0%
! TACTTTTGAACAGGGAATGG+AGG + chr5.1:7475040-7475059 MS.gene57419:intron 40.0%
! TTCAGGAAGTGGTTGGTTAT+TGG - chr5.1:7476359-7476378 None:intergenic 40.0%
!! AGAAGCAATTGGTGTTGAAC+TGG - chr5.1:7475279-7475298 None:intergenic 40.0%
!! AGGGCAAAAAGGTACTACTT+TGG - chr5.1:7476492-7476511 None:intergenic 40.0%
ACAAGTTCAGGCACATAGGT+GGG - chr5.1:7474817-7474836 None:intergenic 45.0%
CAAGAGAATCCATCGGGTAT+TGG - chr5.1:7475082-7475101 None:intergenic 45.0%
CATTGTTCCTGGCCTCAAAA+AGG + chr5.1:7475538-7475557 MS.gene57419:CDS 45.0%
CTTCGATTTCAGGAAGTGGT+TGG - chr5.1:7476366-7476385 None:intergenic 45.0%
GAACCTTGAGAAACCAAAGC+TGG - chr5.1:7475380-7475399 None:intergenic 45.0%
GAGATGCAAGAGAATCCATC+GGG - chr5.1:7475088-7475107 None:intergenic 45.0%
GATGGACAAAGCTTCTGTGT+TGG + chr5.1:7476183-7476202 MS.gene57419:intron 45.0%
GCTTACAAGTTCAGGCACAT+AGG - chr5.1:7474821-7474840 None:intergenic 45.0%
GGAGATGCAAGAGAATCCAT+CGG - chr5.1:7475089-7475108 None:intergenic 45.0%
TACAAGTTCAGGCACATAGG+TGG - chr5.1:7474818-7474837 None:intergenic 45.0%
TGTTCCTGGTCTGATTCACT+TGG - chr5.1:7475439-7475458 None:intergenic 45.0%
! CTTCCAGCTTTGGTTTCTCA+AGG + chr5.1:7475374-7475393 MS.gene57419:CDS 45.0%
!!! CTTTTTGAGGCCAGGAACAA+TGG - chr5.1:7475540-7475559 None:intergenic 45.0%
!!! GATCATACCTTTTTGAGGCC+AGG - chr5.1:7475548-7475567 None:intergenic 45.0%
AAGTTCAGGCACATAGGTGG+GGG - chr5.1:7474815-7474834 None:intergenic 50.0%
ACAAGCACGTTTGCAGACAC+TGG + chr5.1:7476234-7476253 MS.gene57419:CDS 50.0%
CAAGTTCAGGCACATAGGTG+GGG - chr5.1:7474816-7474835 None:intergenic 50.0%
GCTCTTTCAGCCATTGTTCC+TGG + chr5.1:7475527-7475546 MS.gene57419:CDS 50.0%
! TAATTGGCCAAGCACCGTAG+CGG - chr5.1:7476449-7476468 None:intergenic 50.0%
AGCACGTTTGCAGACACTGG+AGG + chr5.1:7476237-7476256 MS.gene57419:CDS 55.0%
TCGAGCTTGAGCAGTGTTCC+TGG - chr5.1:7475453-7475472 None:intergenic 55.0%
! ACACAGTGGACGTGCCGCTA+CGG + chr5.1:7476432-7476451 MS.gene57419:CDS 60.0%
! GGACGTGCCGCTACGGTGCT+TGG + chr5.1:7476439-7476458 MS.gene57419:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 7474742 7476549 7474742 ID=MS.gene57419
chr5.1 mRNA 7474742 7476549 7474742 ID=MS.gene57419.t1;Parent=MS.gene57419
chr5.1 exon 7474742 7474834 7474742 ID=MS.gene57419.t1.exon1;Parent=MS.gene57419.t1
chr5.1 CDS 7474742 7474834 7474742 ID=cds.MS.gene57419.t1;Parent=MS.gene57419.t1
chr5.1 exon 7475053 7475559 7475053 ID=MS.gene57419.t1.exon2;Parent=MS.gene57419.t1
chr5.1 CDS 7475053 7475559 7475053 ID=cds.MS.gene57419.t1;Parent=MS.gene57419.t1
chr5.1 exon 7476184 7476549 7476184 ID=MS.gene57419.t1.exon3;Parent=MS.gene57419.t1
chr5.1 CDS 7476184 7476549 7476184 ID=cds.MS.gene57419.t1;Parent=MS.gene57419.t1
Gene Sequence

>MS.gene57419

TGCATGTCCCATACCACATCTAATCAAATCGAAGTTTTAGGAAAGAAACTGAAGATGATGCAAATTATGTCCCCCACCTATGTGCCTGAACTTGTAAGCATACTACTATCATCACCTATCTTAAAATTCATAAACTTTAAGTAATCACAATTATAGCTGAACTTGAAGTATTGAAGTGATTCATCTTAAAATCTTAGCTCTCGAATAATTTTTGACCAAGCTTTATTTTTCTGGTCGAACTTTGGATTTGAAGAAATGAAGTTGCATGTGTATAAAAGAAGAAAGCTTACTCTTTAATTACTTTTGAACAGGGAATGGAGGATCTCAACTTCTTTAACCAATACCCGATGGATTCTCTTGCATCTCCAATATTATTCGACAACTTTGGCTTTCACTTTGATGACAACGAAACCACACCAAATTGTTTCCCAGTTGAAACTCATCTTGATGATCAAACAAGGCCAACCAAGAAGATCAAAACATCGATAAACCCTTCTTCCTATTCATCACCTCAATTCATTTCTTTTGAACACTCCAGTTCAACACCAATTGCTTCTAAGCAATTTTATAACCTTGATTATTCCGATGTAAAACCTAAAGTCGAGAAAAGGTGTAATGATAACAAAGATTTTCTTCCAGCTTTGGTTTCTCAAGGTTCTTATGAAGATCAAAAAATATTTTCAAATTATGATAACCAAGTGAATCAGACCAGGAACACTGCTCAAGCTCGAGAACATGTAATGGCCGAAAGAAAACGAAGGGAAAAGCTTACCCGAAGTTTCATCGCTCTTTCAGCCATTGTTCCTGGCCTCAAAAAGGTATGATCTTATCTCCAACTCACAAACATGTTATAATGTAACTCCATTCAAACCATTTCTATATCAACATACTACTAGAAAAAGAAACATTAATGATCCATATTAAAGGCTGAAATGGTGTGATCTCTCTAATTCTCTAGTCCCTAGCAATATTAGTGACCATTTCATGTTTTTCGTCAATAATTTATTTTTCTTTTGGAATGAAGACTTGTTTTAATTTCTGAGAAAAACAGTATAACCTAATCTGATCATAAGAAAATAAAATCAATTTTTAGTCTTCGAGAAAAAAATAGGACAGCTTTTACTACTCAGACACAAAGAGTAGACATCAAAAAGTTGGGGATTAAATTGTTCTAGTTTCTTTTCTCAGAAACTAAATTGTTTTTATTTCATTTTCTAGGATCAAATCAGGTATCTAATTTTTTTATAATTTAAATTTTATAATTGTGAGTAATGTTCTACTTACACTAGGTGCAACTAATTTCAGTAGTACTAAATTACTGATTAGTGAATAAACACACATGCAAGCTTAACCTAATTAAGTTGAACTTACCAATTGCAATATTTATATTGTACTAGATTTGAATGAATAAATTAATGTATTCAATATTATGGTATGTGCAGATGGACAAAGCTTCTGTGTTGGGAGATGCTATCAAGTACATGAAACAACTACAAGCACGTTTGCAGACACTGGAGGAACAAGCTGAAGACAACAAAAAAACTGGATCCACAGTTCAAGTGAAGAGATCTATTATCTTCACTAATGATGACGATGATGATGATGATAGCAACTCCAATAACCAACCACTTCCTGAAATCGAAGTAAGAGTATCAAGCAAAGATGTACTCATCAAGATTCAATGTGACAAACACAGTGGACGTGCCGCTACGGTGCTTGGCCAATTAGAAAACCTTAATCTCACTGTCCAAAGTAGTACCTTTTTGCCCTTTGGAAACAACATTGTTGATGTAACCATTGTTGCTCAG
Protein sequence

>MS.gene57419.t1

CMSHTTSNQIEVLGKKLKMMQIMSPTYVPELGMEDLNFFNQYPMDSLASPILFDNFGFHFDDNETTPNCFPVETHLDDQTRPTKKIKTSINPSSYSSPQFISFEHSSSTPIASKQFYNLDYSDVKPKVEKRCNDNKDFLPALVSQGSYEDQKIFSNYDNQVNQTRNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKMDKASVLGDAIKYMKQLQARLQTLEEQAEDNKKTGSTVQVKRSIIFTNDDDDDDDSNSNNQPLPEIEVRVSSKDVLIKIQCDKHSGRAATVLGQLENLNLTVQSSTFLPFGNNIVDVTIVAQ