Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57606.t1 | XP_024637947.1 | 100 | 72 | 0 | 0 | 1 | 72 | 147 | 218 | 5.90E-34 | 152.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57606.t1 | Q9LVW2 | 86.0 | 50 | 7 | 0 | 1 | 50 | 117 | 166 | 1.0e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57606.t1 | A0A396IIY0 | 100.0 | 72 | 0 | 0 | 1 | 72 | 147 | 218 | 4.3e-34 | 152.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene57606.t1 | TF | HSF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57606.t1 | MTR_1g061170 | 55.556 | 81 | 31 | 1 | 1 | 76 | 95 | 175 | 1.06e-22 | 89.7 |
| MS.gene57606.t1 | MTR_1g071360 | 56.757 | 74 | 24 | 2 | 1 | 72 | 78 | 145 | 1.07e-22 | 89.0 |
| MS.gene57606.t1 | MTR_1g071360 | 56.757 | 74 | 24 | 2 | 1 | 72 | 75 | 142 | 1.54e-22 | 88.6 |
| MS.gene57606.t1 | MTR_5g029680 | 53.488 | 86 | 26 | 2 | 1 | 72 | 78 | 163 | 2.68e-22 | 88.6 |
| MS.gene57606.t1 | MTR_4g077970 | 58.824 | 85 | 22 | 3 | 1 | 72 | 58 | 142 | 3.51e-22 | 88.2 |
| MS.gene57606.t1 | MTR_3g104550 | 55.556 | 81 | 29 | 2 | 1 | 74 | 92 | 172 | 1.34e-21 | 86.3 |
| MS.gene57606.t1 | MTR_8g083100 | 56.000 | 75 | 30 | 1 | 1 | 72 | 68 | 142 | 6.72e-21 | 84.7 |
| MS.gene57606.t1 | MTR_8g105780 | 80.000 | 45 | 9 | 0 | 1 | 45 | 69 | 113 | 7.78e-21 | 84.0 |
| MS.gene57606.t1 | MTR_7g095680 | 80.000 | 45 | 9 | 0 | 1 | 45 | 95 | 139 | 9.06e-21 | 84.0 |
| MS.gene57606.t1 | MTR_6g086805 | 82.222 | 45 | 8 | 0 | 1 | 45 | 57 | 101 | 1.62e-20 | 82.8 |
| MS.gene57606.t1 | MTR_1g102860 | 53.488 | 86 | 26 | 4 | 1 | 76 | 86 | 167 | 2.57e-20 | 82.4 |
| MS.gene57606.t1 | MTR_2g100680 | 56.000 | 75 | 30 | 1 | 1 | 72 | 77 | 151 | 3.28e-20 | 82.8 |
| MS.gene57606.t1 | MTR_8g087540 | 53.086 | 81 | 31 | 1 | 1 | 74 | 58 | 138 | 4.00e-20 | 82.4 |
| MS.gene57606.t1 | MTR_6g043060 | 56.164 | 73 | 29 | 1 | 1 | 70 | 68 | 140 | 6.69e-20 | 81.3 |
| MS.gene57606.t1 | MTR_5g010680 | 75.556 | 45 | 11 | 0 | 1 | 45 | 70 | 114 | 7.94e-20 | 81.3 |
| MS.gene57606.t1 | MTR_3g101870 | 65.517 | 58 | 19 | 1 | 1 | 58 | 70 | 126 | 2.65e-19 | 79.7 |
| MS.gene57606.t1 | MTR_2g017890 | 50.685 | 73 | 33 | 1 | 1 | 70 | 61 | 133 | 1.35e-18 | 77.8 |
| MS.gene57606.t1 | MTR_5g089170 | 73.333 | 45 | 12 | 0 | 1 | 45 | 71 | 115 | 9.19e-18 | 74.3 |
| MS.gene57606.t1 | MTR_2g100670 | 53.947 | 76 | 30 | 3 | 1 | 72 | 187 | 261 | 3.16e-17 | 74.3 |
| MS.gene57606.t1 | MTR_7g091370 | 60.377 | 53 | 21 | 0 | 1 | 53 | 68 | 120 | 3.61e-17 | 72.4 |
| MS.gene57606.t1 | MTR_1g100777 | 68.889 | 45 | 14 | 0 | 1 | 45 | 64 | 108 | 8.55e-17 | 72.4 |
| MS.gene57606.t1 | MTR_0020s0020 | 73.333 | 45 | 12 | 0 | 1 | 45 | 79 | 123 | 1.15e-16 | 72.4 |
| MS.gene57606.t1 | MTR_5g017470 | 68.889 | 45 | 14 | 0 | 1 | 45 | 55 | 99 | 1.22e-16 | 72.0 |
| MS.gene57606.t1 | MTR_2g100670 | 52.000 | 75 | 33 | 1 | 1 | 72 | 79 | 153 | 2.12e-16 | 71.6 |
| MS.gene57606.t1 | MTR_4g088900 | 66.667 | 45 | 15 | 0 | 1 | 45 | 83 | 127 | 8.50e-16 | 68.6 |
| MS.gene57606.t1 | MTR_1g061170 | 45.714 | 70 | 33 | 1 | 12 | 76 | 11 | 80 | 1.21e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57606.t1 | AT5G54070 | 86.000 | 50 | 7 | 0 | 1 | 50 | 117 | 166 | 4.00e-25 | 95.1 |
| MS.gene57606.t1 | AT2G26150 | 60.494 | 81 | 21 | 3 | 1 | 72 | 90 | 168 | 7.12e-24 | 92.0 |
| MS.gene57606.t1 | AT2G26150 | 60.494 | 81 | 21 | 3 | 1 | 72 | 90 | 168 | 7.12e-24 | 92.0 |
| MS.gene57606.t1 | AT3G22830 | 81.633 | 49 | 9 | 0 | 1 | 49 | 107 | 155 | 3.06e-22 | 88.2 |
| MS.gene57606.t1 | AT5G03720 | 58.228 | 79 | 28 | 2 | 1 | 74 | 101 | 179 | 3.57e-22 | 88.2 |
| MS.gene57606.t1 | AT5G03720 | 58.228 | 79 | 28 | 2 | 1 | 74 | 101 | 179 | 3.57e-22 | 88.2 |
| MS.gene57606.t1 | AT4G17750 | 84.444 | 45 | 7 | 0 | 1 | 45 | 98 | 142 | 4.62e-22 | 88.2 |
| MS.gene57606.t1 | AT1G32330 | 55.682 | 88 | 23 | 3 | 1 | 72 | 83 | 170 | 8.23e-22 | 87.4 |
| MS.gene57606.t1 | AT1G32330 | 55.682 | 88 | 23 | 3 | 1 | 72 | 83 | 170 | 9.14e-22 | 87.4 |
| MS.gene57606.t1 | AT3G24520 | 59.722 | 72 | 27 | 1 | 1 | 72 | 63 | 132 | 1.53e-21 | 85.5 |
| MS.gene57606.t1 | AT5G16820 | 82.222 | 45 | 8 | 0 | 1 | 45 | 73 | 117 | 1.69e-20 | 83.6 |
| MS.gene57606.t1 | AT5G16820 | 82.222 | 45 | 8 | 0 | 1 | 45 | 73 | 117 | 1.69e-20 | 83.6 |
| MS.gene57606.t1 | AT3G02990 | 80.000 | 45 | 9 | 0 | 1 | 45 | 69 | 113 | 4.18e-20 | 82.4 |
| MS.gene57606.t1 | AT5G43840 | 50.000 | 76 | 31 | 1 | 1 | 76 | 65 | 133 | 4.19e-20 | 81.3 |
| MS.gene57606.t1 | AT3G51910 | 67.347 | 49 | 16 | 0 | 1 | 49 | 75 | 123 | 3.51e-19 | 78.6 |
| MS.gene57606.t1 | AT1G67970 | 67.925 | 53 | 17 | 0 | 1 | 53 | 66 | 118 | 3.82e-19 | 79.3 |
| MS.gene57606.t1 | AT1G46264 | 66.038 | 53 | 18 | 0 | 1 | 53 | 80 | 132 | 7.35e-19 | 78.6 |
| MS.gene57606.t1 | AT4G18880 | 55.224 | 67 | 29 | 1 | 1 | 66 | 61 | 127 | 7.83e-19 | 79.0 |
| MS.gene57606.t1 | AT3G63350 | 69.565 | 46 | 14 | 0 | 1 | 46 | 74 | 119 | 1.77e-18 | 76.6 |
| MS.gene57606.t1 | AT4G13980 | 75.556 | 45 | 11 | 0 | 1 | 45 | 69 | 113 | 1.87e-18 | 77.8 |
| MS.gene57606.t1 | AT5G45710 | 50.000 | 78 | 32 | 1 | 1 | 71 | 59 | 136 | 2.13e-18 | 77.4 |
| MS.gene57606.t1 | AT5G45710 | 50.000 | 78 | 32 | 1 | 1 | 71 | 59 | 136 | 2.13e-18 | 77.4 |
| MS.gene57606.t1 | AT5G62020 | 64.151 | 53 | 18 | 1 | 1 | 53 | 69 | 120 | 5.69e-17 | 72.8 |
| MS.gene57606.t1 | AT4G11660 | 71.111 | 45 | 13 | 0 | 1 | 45 | 105 | 149 | 5.84e-17 | 73.6 |
| MS.gene57606.t1 | AT2G41690 | 68.889 | 45 | 14 | 0 | 1 | 45 | 86 | 130 | 2.67e-16 | 70.5 |
| MS.gene57606.t1 | AT4G36990 | 71.111 | 45 | 13 | 0 | 1 | 45 | 60 | 104 | 3.01e-16 | 70.9 |
| MS.gene57606.t1 | AT2G26150 | 53.731 | 67 | 20 | 3 | 15 | 72 | 49 | 113 | 1.92e-14 | 65.9 |
| MS.gene57606.t1 | AT2G26150 | 53.731 | 67 | 20 | 3 | 15 | 72 | 49 | 113 | 1.92e-14 | 65.9 |
Find 14 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGTCACATGCAGGGGTTT+AGG | 0.362833 | 8.4:-9060478 | MS.gene57606:intron |
| CGTTGGGAGTTTGCAAATGA+AGG | 0.392464 | 8.4:-9060440 | MS.gene57606:CDS |
| GAAGGTTGATTCAGACCGTT+GGG | 0.482934 | 8.4:-9060456 | MS.gene57606:CDS |
| CAAATGAAGGATTTCAAGCA+GGG | 0.484602 | 8.4:-9060427 | MS.gene57606:CDS |
| GACTTACATAGGTGTTGAGC+TGG | 0.485810 | 8.4:+9061277 | None:intergenic |
| ATTTGTTGAAGAACATAAGA+AGG | 0.488953 | 8.4:-9060397 | MS.gene57606:CDS |
| GCAAATGAAGGATTTCAAGC+AGG | 0.502811 | 8.4:-9060428 | MS.gene57606:CDS |
| GGAAGGTTGATTCAGACCGT+TGG | 0.515312 | 8.4:-9060457 | MS.gene57606:CDS |
| TCCACTTCAGATTCAACGCA+AGG | 0.559576 | 8.4:+9060326 | None:intergenic |
| CTTCATTTGCAAACTCCCAA+CGG | 0.561522 | 8.4:+9060441 | None:intergenic |
| TCACATGCAGGGGTTTAGGA+AGG | 0.572288 | 8.4:-9060474 | MS.gene57606:intron |
| AAGTATAACAAACTGCATCA+AGG | 0.578236 | 8.4:-9060368 | MS.gene57606:CDS |
| TCCTTGCGTTGAATCTGAAG+TGG | 0.604555 | 8.4:-9060327 | MS.gene57606:CDS |
| TATAACAAACTGCATCAAGG+AGG | 0.715319 | 8.4:-9060365 | MS.gene57606:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATGATTCATATTTAT+AGG | - | chr8.4:9060512-9060531 | MS.gene57606:intron | 10.0% |
| !! | TAAAATGATTCATATTTATA+GGG | - | chr8.4:9060513-9060532 | MS.gene57606:intron | 10.0% |
| !!! | TTTTTTCATGTTTTTTTACA+TGG | + | chr8.4:9060814-9060833 | None:intergenic | 15.0% |
| !! | AAATCCAGAATAAATTCAAA+TGG | + | chr8.4:9060867-9060886 | None:intergenic | 20.0% |
| !!! | GAAATTTTTTTGACTTACAT+AGG | + | chr8.4:9060338-9060357 | None:intergenic | 20.0% |
| !!! | GCAAAAAACAAAATAGATTT+TGG | + | chr8.4:9060946-9060965 | None:intergenic | 20.0% |
| !!! | TGTACAATTTAAATCAGTTT+TGG | - | chr8.4:9060403-9060422 | MS.gene57606:CDS | 20.0% |
| ! | ACATTAAATTCGAACTTCAT+TGG | - | chr8.4:9060759-9060778 | MS.gene57606:intron | 25.0% |
| !! | ATTTGTTGAAGAACATAAGA+AGG | - | chr8.4:9061204-9061223 | MS.gene57606:intron | 25.0% |
| !!! | TTACATGAACTCACTTTTAT+AGG | - | chr8.4:9060718-9060737 | MS.gene57606:intron | 25.0% |
| !!! | TTTTTTGCAACCACAAAAAT+AGG | - | chr8.4:9060958-9060977 | MS.gene57606:intron | 25.0% |
| AAATCAACTCAATGCATGTT+TGG | + | chr8.4:9060457-9060476 | None:intergenic | 30.0% | |
| AAGTATAACAAACTGCATCA+AGG | - | chr8.4:9061233-9061252 | MS.gene57606:intron | 30.0% | |
| AATGCATGTTTGGTATAATG+TGG | + | chr8.4:9060447-9060466 | None:intergenic | 30.0% | |
| TTCGCCATTTGAATTTATTC+TGG | - | chr8.4:9060860-9060879 | MS.gene57606:intron | 30.0% | |
| !! | TTAAATCAGTTTTGGCATCA+CGG | - | chr8.4:9060411-9060430 | MS.gene57606:CDS | 30.0% |
| CAAATGAAGGATTTCAAGCA+GGG | - | chr8.4:9061174-9061193 | MS.gene57606:intron | 35.0% | |
| TATAACAAACTGCATCAAGG+AGG | - | chr8.4:9061236-9061255 | MS.gene57606:intron | 35.0% | |
| TATAGGGAGCTATGAATGTT+TGG | - | chr8.4:9060529-9060548 | MS.gene57606:intron | 35.0% | |
| TTATGAATCTGTCACATGCA+GGG | - | chr8.4:9061116-9061135 | MS.gene57606:intron | 35.0% | |
| TTTATGAATCTGTCACATGC+AGG | - | chr8.4:9061115-9061134 | MS.gene57606:intron | 35.0% | |
| TTTGCAACCACAAAAATAGG+TGG | - | chr8.4:9060961-9060980 | MS.gene57606:intron | 35.0% | |
| ! | TTCAACATCTCCTCTAAAAG+AGG | + | chr8.4:9060557-9060576 | None:intergenic | 35.0% |
| !! | ATGTTTGGTTCCTCTTTTAG+AGG | - | chr8.4:9060544-9060563 | MS.gene57606:intron | 35.0% |
| !!! | TTAGTCTCCACCTATTTTTG+TGG | + | chr8.4:9060971-9060990 | None:intergenic | 35.0% |
| CTTCATTTGCAAACTCCCAA+CGG | + | chr8.4:9061163-9061182 | None:intergenic | 40.0% | |
| GCAAATGAAGGATTTCAAGC+AGG | - | chr8.4:9061173-9061192 | MS.gene57606:intron | 40.0% | |
| GGTGGAGACTAAAAATCAAG+AGG | - | chr8.4:9060979-9060998 | MS.gene57606:intron | 40.0% | |
| TATGAATCTGTCACATGCAG+GGG | - | chr8.4:9061117-9061136 | MS.gene57606:intron | 40.0% | |
| CGTTGGGAGTTTGCAAATGA+AGG | - | chr8.4:9061161-9061180 | MS.gene57606:intron | 45.0% | |
| TCCACTTCAGATTCAACGCA+AGG | + | chr8.4:9061278-9061297 | None:intergenic | 45.0% | |
| TCCTTGCGTTGAATCTGAAG+TGG | - | chr8.4:9061274-9061293 | MS.gene57606:intron | 45.0% | |
| ! | GAAGGTTGATTCAGACCGTT+GGG | - | chr8.4:9061145-9061164 | MS.gene57606:intron | 45.0% |
| !! | GACTTACATAGGTGTTGAGC+TGG | + | chr8.4:9060327-9060346 | None:intergenic | 45.0% |
| TCACATGCAGGGGTTTAGGA+AGG | - | chr8.4:9061127-9061146 | MS.gene57606:intron | 50.0% | |
| TCTGTCACATGCAGGGGTTT+AGG | - | chr8.4:9061123-9061142 | MS.gene57606:intron | 50.0% | |
| ! | GGAAGGTTGATTCAGACCGT+TGG | - | chr8.4:9061144-9061163 | MS.gene57606:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9060298 | 9061325 | 9060298 | ID=MS.gene57606 |
| chr8.4 | mRNA | 9060298 | 9061325 | 9060298 | ID=MS.gene57606.t1;Parent=MS.gene57606 |
| chr8.4 | exon | 9061284 | 9061325 | 9061284 | ID=MS.gene57606.t1.exon1;Parent=MS.gene57606.t1 |
| chr8.4 | CDS | 9061284 | 9061325 | 9061284 | ID=cds.MS.gene57606.t1;Parent=MS.gene57606.t1 |
| chr8.4 | exon | 9060298 | 9060486 | 9060298 | ID=MS.gene57606.t1.exon2;Parent=MS.gene57606.t1 |
| chr8.4 | CDS | 9060298 | 9060486 | 9060298 | ID=cds.MS.gene57606.t1;Parent=MS.gene57606.t1 |
| Gene Sequence |
| Protein sequence |
