Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57608.t1 | XP_003610006.1 | 75 | 100 | 21 | 2 | 1 | 100 | 43 | 138 | 8.70E-17 | 96.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57608.t1 | P46602 | 71.7 | 46 | 13 | 0 | 55 | 100 | 167 | 212 | 1.2e-11 | 70.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57608.t1 | G7JT79 | 75.0 | 100 | 21 | 2 | 1 | 100 | 43 | 138 | 6.2e-17 | 96.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene57608.t1 | TF | HB-other |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57608.t1 | MTR_4g126900 | 91.000 | 100 | 5 | 2 | 1 | 100 | 43 | 138 | 9.23e-51 | 159 |
| MS.gene57608.t1 | MTR_5g013010 | 54.878 | 82 | 30 | 1 | 19 | 100 | 112 | 186 | 2.06e-22 | 86.7 |
| MS.gene57608.t1 | MTR_5g013010 | 54.878 | 82 | 30 | 1 | 19 | 100 | 112 | 186 | 9.97e-22 | 86.7 |
| MS.gene57608.t1 | MTR_5g014890 | 69.841 | 63 | 15 | 1 | 38 | 100 | 115 | 173 | 5.62e-21 | 84.3 |
| MS.gene57608.t1 | MTR_4g100550 | 62.319 | 69 | 20 | 1 | 38 | 100 | 102 | 170 | 9.95e-21 | 84.0 |
| MS.gene57608.t1 | MTR_8g006705 | 78.846 | 52 | 11 | 0 | 49 | 100 | 145 | 196 | 2.05e-20 | 83.2 |
| MS.gene57608.t1 | MTR_7g093430 | 51.613 | 93 | 40 | 2 | 8 | 100 | 123 | 210 | 2.38e-20 | 83.2 |
| MS.gene57608.t1 | MTR_3g103590 | 67.188 | 64 | 20 | 1 | 37 | 100 | 133 | 195 | 3.03e-20 | 82.8 |
| MS.gene57608.t1 | MTR_1g054285 | 50.538 | 93 | 40 | 2 | 8 | 100 | 118 | 204 | 7.78e-20 | 81.6 |
| MS.gene57608.t1 | MTR_2g038625 | 67.213 | 61 | 20 | 0 | 40 | 100 | 72 | 132 | 1.04e-19 | 79.7 |
| MS.gene57608.t1 | MTR_2g038580 | 67.213 | 61 | 20 | 0 | 40 | 100 | 72 | 132 | 1.04e-19 | 79.7 |
| MS.gene57608.t1 | MTR_2g061030 | 60.938 | 64 | 25 | 0 | 37 | 100 | 187 | 250 | 1.51e-19 | 81.3 |
| MS.gene57608.t1 | MTR_4g097600 | 73.077 | 52 | 14 | 0 | 49 | 100 | 126 | 177 | 4.62e-19 | 79.3 |
| MS.gene57608.t1 | MTR_1g017090 | 52.439 | 82 | 34 | 1 | 19 | 100 | 104 | 180 | 2.38e-18 | 77.4 |
| MS.gene57608.t1 | MTR_4g084750 | 61.667 | 60 | 23 | 0 | 41 | 100 | 56 | 115 | 3.54e-18 | 75.5 |
| MS.gene57608.t1 | MTR_8g095670 | 77.049 | 61 | 10 | 2 | 1 | 61 | 125 | 181 | 9.42e-17 | 71.6 |
| MS.gene57608.t1 | MTR_8g469430 | 54.902 | 51 | 23 | 0 | 50 | 100 | 89 | 139 | 6.11e-12 | 60.1 |
| MS.gene57608.t1 | MTR_5g038280 | 50.980 | 51 | 25 | 0 | 50 | 100 | 87 | 137 | 1.67e-11 | 58.9 |
| MS.gene57608.t1 | MTR_6g007647 | 56.863 | 51 | 22 | 0 | 50 | 100 | 33 | 83 | 1.69e-11 | 58.5 |
| MS.gene57608.t1 | MTR_6g007647 | 56.863 | 51 | 22 | 0 | 50 | 100 | 67 | 117 | 1.90e-11 | 58.9 |
| MS.gene57608.t1 | MTR_7g103340 | 44.286 | 70 | 39 | 0 | 31 | 100 | 44 | 113 | 3.44e-11 | 57.8 |
| MS.gene57608.t1 | MTR_7g010020 | 50.000 | 52 | 26 | 0 | 49 | 100 | 79 | 130 | 5.55e-11 | 57.4 |
| MS.gene57608.t1 | MTR_3g080100 | 50.980 | 51 | 25 | 0 | 50 | 100 | 20 | 70 | 8.69e-11 | 56.2 |
| MS.gene57608.t1 | MTR_3g080100 | 50.980 | 51 | 25 | 0 | 50 | 100 | 59 | 109 | 9.90e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57608.t1 | AT3G60390 | 66.667 | 66 | 21 | 1 | 36 | 100 | 147 | 212 | 1.56e-22 | 89.0 |
| MS.gene57608.t1 | AT5G06710 | 63.077 | 65 | 24 | 0 | 36 | 100 | 176 | 240 | 2.92e-22 | 87.8 |
| MS.gene57608.t1 | AT5G06710 | 63.077 | 65 | 24 | 0 | 36 | 100 | 176 | 240 | 8.88e-22 | 87.4 |
| MS.gene57608.t1 | AT4G37790 | 62.857 | 70 | 24 | 1 | 33 | 100 | 107 | 176 | 3.49e-21 | 85.1 |
| MS.gene57608.t1 | AT4G16780 | 66.129 | 62 | 20 | 1 | 39 | 100 | 119 | 179 | 3.65e-20 | 82.4 |
| MS.gene57608.t1 | AT4G17460 | 45.833 | 96 | 36 | 2 | 5 | 100 | 106 | 185 | 4.76e-20 | 82.0 |
| MS.gene57608.t1 | AT5G47370 | 60.000 | 65 | 26 | 0 | 36 | 100 | 116 | 180 | 4.83e-20 | 82.0 |
| MS.gene57608.t1 | AT2G44910 | 71.154 | 52 | 15 | 0 | 49 | 100 | 162 | 213 | 6.50e-19 | 79.3 |
| MS.gene57608.t1 | AT2G22800 | 71.154 | 52 | 15 | 0 | 49 | 100 | 112 | 163 | 2.39e-18 | 77.4 |
| MS.gene57608.t1 | AT1G70920 | 51.316 | 76 | 37 | 0 | 25 | 100 | 14 | 89 | 4.37e-18 | 74.7 |
| MS.gene57608.t1 | AT1G70920 | 51.316 | 76 | 37 | 0 | 25 | 100 | 44 | 119 | 6.03e-18 | 75.1 |
| MS.gene57608.t1 | AT2G01430 | 55.714 | 70 | 26 | 1 | 36 | 100 | 120 | 189 | 1.19e-16 | 72.0 |
| MS.gene57608.t1 | AT2G01430 | 55.714 | 70 | 26 | 1 | 36 | 100 | 120 | 189 | 1.93e-16 | 72.4 |
| MS.gene57608.t1 | AT3G01470 | 54.902 | 51 | 23 | 0 | 50 | 100 | 68 | 118 | 5.93e-11 | 57.4 |
| MS.gene57608.t1 | AT3G01220 | 48.387 | 62 | 32 | 0 | 39 | 100 | 76 | 137 | 9.82e-11 | 57.0 |
Find 17 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAGGTGAAAGATTCAATT+TGG | 0.267895 | 8.4:+8059549 | MS.gene57608:CDS |
| ACGTTCATAGGTTCTCCTTT+TGG | 0.352374 | 8.4:-8059505 | None:intergenic |
| TTGAGGTTTGGTTTCAGAAC+AGG | 0.362659 | 8.4:+8060082 | MS.gene57608:CDS |
| GTCTTGTCTTTAACCTCAAT+TGG | 0.365222 | 8.4:-8060056 | None:intergenic |
| TTGTTGTTGTGTACGTTCAT+AGG | 0.371543 | 8.4:-8059517 | None:intergenic |
| TCTACGATCGTTGTTGTTGT+TGG | 0.376794 | 8.4:-8059623 | None:intergenic |
| AGCTTTGAGCTTTGTCCAAA+AGG | 0.402369 | 8.4:+8059490 | None:intergenic |
| AGACAAGACAAATTGAGGTT+TGG | 0.409339 | 8.4:+8060070 | MS.gene57608:CDS |
| TAGCACTTGCTGACCAATTG+AGG | 0.458161 | 8.4:+8060043 | MS.gene57608:CDS |
| GTTAAAGACAAGACAAATTG+AGG | 0.472426 | 8.4:+8060065 | MS.gene57608:CDS |
| ATACCGGATTAAGAGTGTTA+TGG | 0.475608 | 8.4:-8059702 | None:intergenic |
| GCTCCATAACACTCTTAATC+CGG | 0.495490 | 8.4:+8059699 | MS.gene57608:CDS |
| AAGAGTGTTATGGAGCTTGA+AGG | 0.507942 | 8.4:-8059692 | None:intergenic |
| CTTGAAGGTGCTCTCAAGCA+TGG | 0.539476 | 8.4:-8059677 | None:intergenic |
| GGTTTCAGAACAGGAGAGCA+AGG | 0.572572 | 8.4:+8060091 | MS.gene57608:CDS |
| AAAGAAACTGAGGCTAACAA+AGG | 0.656758 | 8.4:+8059645 | MS.gene57608:CDS |
| ACGATCGTAGAAAGAAACTG+AGG | 0.720750 | 8.4:+8059635 | MS.gene57608:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTATTATTATTTTTAA+TGG | + | chr8.4:8059888-8059907 | MS.gene57608:intron | 0.0% |
| !!! | TACATATTATAGAGAATTTT+TGG | - | chr8.4:8059745-8059764 | None:intergenic | 15.0% |
| !!! | ACGTTACATTTTGATAATAT+TGG | - | chr8.4:8059582-8059601 | None:intergenic | 20.0% |
| ! | TGTCATATTGTGTATGTATA+AGG | + | chr8.4:8059787-8059806 | MS.gene57608:intron | 25.0% |
| !! | TTTTGGTATGAATGAATGAA+TGG | - | chr8.4:8059728-8059747 | None:intergenic | 25.0% |
| !!! | ATTAGTTTTTCTAGATGACA+AGG | + | chr8.4:8059965-8059984 | MS.gene57608:intron | 25.0% |
| !!! | TTAGTTTTTCTAGATGACAA+GGG | + | chr8.4:8059966-8059985 | MS.gene57608:intron | 25.0% |
| !!! | TTTTGGTTTCATATATATGC+AGG | + | chr8.4:8060011-8060030 | MS.gene57608:intron | 25.0% |
| ATGAATGAATGAATGGATAC+CGG | - | chr8.4:8059721-8059740 | None:intergenic | 30.0% | |
| CAAAGGTGAAAGATTCAATT+TGG | + | chr8.4:8059549-8059568 | MS.gene57608:CDS | 30.0% | |
| GTTAAAGACAAGACAAATTG+AGG | + | chr8.4:8060065-8060084 | MS.gene57608:CDS | 30.0% | |
| AAAGAAACTGAGGCTAACAA+AGG | + | chr8.4:8059645-8059664 | MS.gene57608:CDS | 35.0% | |
| AACAACAACAACAACAGCAA+AGG | + | chr8.4:8059532-8059551 | MS.gene57608:CDS | 35.0% | |
| AGACAAGACAAATTGAGGTT+TGG | + | chr8.4:8060070-8060089 | MS.gene57608:CDS | 35.0% | |
| GTAGAAATGAGTTTATGCGT+TGG | + | chr8.4:8059988-8060007 | MS.gene57608:intron | 35.0% | |
| GTCTTGTCTTTAACCTCAAT+TGG | - | chr8.4:8060059-8060078 | None:intergenic | 35.0% | |
| ! | TTGTTGTTGTGTACGTTCAT+AGG | - | chr8.4:8059520-8059539 | None:intergenic | 35.0% |
| !! | ATACCGGATTAAGAGTGTTA+TGG | - | chr8.4:8059705-8059724 | None:intergenic | 35.0% |
| !!! | ATGAGTTTATGCGTTGGTTT+TGG | + | chr8.4:8059994-8060013 | MS.gene57608:intron | 35.0% |
| ACGATCGTAGAAAGAAACTG+AGG | + | chr8.4:8059635-8059654 | MS.gene57608:CDS | 40.0% | |
| GCTCCATAACACTCTTAATC+CGG | + | chr8.4:8059699-8059718 | MS.gene57608:CDS | 40.0% | |
| ! | ACGTTCATAGGTTCTCCTTT+TGG | - | chr8.4:8059508-8059527 | None:intergenic | 40.0% |
| ! | TCTACGATCGTTGTTGTTGT+TGG | - | chr8.4:8059626-8059645 | None:intergenic | 40.0% |
| ! | TTGAGGTTTGGTTTCAGAAC+AGG | + | chr8.4:8060082-8060101 | MS.gene57608:CDS | 40.0% |
| !! | AAGAGTGTTATGGAGCTTGA+AGG | - | chr8.4:8059695-8059714 | None:intergenic | 40.0% |
| ! | TAGCACTTGCTGACCAATTG+AGG | + | chr8.4:8060043-8060062 | MS.gene57608:CDS | 45.0% |
| !! | CTTGAAGGTGCTCTCAAGCA+TGG | - | chr8.4:8059680-8059699 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 8059499 | 8060112 | 8059499 | ID=MS.gene57608 |
| chr8.4 | mRNA | 8059499 | 8060112 | 8059499 | ID=MS.gene57608.t1;Parent=MS.gene57608 |
| chr8.4 | exon | 8059499 | 8059720 | 8059499 | ID=MS.gene57608.t1.exon1;Parent=MS.gene57608.t1 |
| chr8.4 | CDS | 8059499 | 8059720 | 8059499 | ID=cds.MS.gene57608.t1;Parent=MS.gene57608.t1 |
| chr8.4 | exon | 8060033 | 8060112 | 8060033 | ID=MS.gene57608.t1.exon2;Parent=MS.gene57608.t1 |
| chr8.4 | CDS | 8060033 | 8060112 | 8060033 | ID=cds.MS.gene57608.t1;Parent=MS.gene57608.t1 |
| Gene Sequence |
| Protein sequence |
