AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57861

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57861.t1 MTR_5g017350 87.970 133 15 1 1 133 188 319 5.92e-80 249
MS.gene57861.t1 MTR_5g017350 87.970 133 15 1 1 133 188 319 4.42e-79 249
MS.gene57861.t1 MTR_5g017350 87.970 133 15 1 1 133 188 319 2.64e-78 249
MS.gene57861.t1 MTR_5g017350 87.970 133 15 1 1 133 188 319 5.21e-78 249
MS.gene57861.t1 MTR_4g022850 63.115 122 44 1 1 122 167 287 2.12e-49 169
MS.gene57861.t1 MTR_4g022850 63.115 122 44 1 1 122 167 287 2.98e-49 169
MS.gene57861.t1 MTR_4g022850 63.115 122 44 1 1 122 167 287 2.99e-49 169
MS.gene57861.t1 MTR_3g087940 72.059 68 16 1 131 198 55 119 9.14e-23 96.3
MS.gene57861.t1 MTR_5g017370 59.016 61 25 0 65 125 12 72 1.85e-16 72.4
MS.gene57861.t1 MTR_8g091650 57.746 71 26 2 129 198 48 115 6.13e-15 73.9
MS.gene57861.t1 MTR_2g086420 80.000 45 8 1 152 195 186 230 1.10e-13 70.9
MS.gene57861.t1 MTR_2g086420 80.000 45 8 1 152 195 187 231 1.14e-13 70.9
MS.gene57861.t1 MTR_7g089800 70.588 51 15 0 148 198 128 178 2.13e-13 69.7
MS.gene57861.t1 MTR_7g089800 70.588 51 15 0 148 198 157 207 2.27e-13 69.7
MS.gene57861.t1 MTR_7g092750 69.231 52 15 1 148 198 163 214 3.81e-13 68.9
MS.gene57861.t1 MTR_7g092750 69.231 52 15 1 148 198 163 214 4.20e-13 68.9
MS.gene57861.t1 MTR_7g092750 69.231 52 15 1 148 198 62 113 4.82e-13 68.6
MS.gene57861.t1 MTR_8g040120 68.627 51 16 0 148 198 171 221 8.73e-13 68.2
MS.gene57861.t1 MTR_0126s0080 69.231 52 15 1 148 198 205 256 9.55e-13 67.8
MS.gene57861.t1 MTR_5g054900 78.049 41 9 0 158 198 149 189 5.50e-12 65.5
MS.gene57861.t1 MTR_5g054900 78.049 41 9 0 158 198 179 219 5.78e-12 65.5
MS.gene57861.t1 MTR_1g111320 50.000 60 30 0 141 200 129 188 6.99e-12 63.5
MS.gene57861.t1 MTR_5g054900 78.049 41 9 0 158 198 68 108 7.70e-12 65.1
MS.gene57861.t1 MTR_5g054900 78.049 41 9 0 158 198 68 108 7.73e-12 64.7
MS.gene57861.t1 MTR_3g044820 50.000 60 30 0 141 200 28 87 1.32e-11 62.8
MS.gene57861.t1 MTR_5g060950 71.429 42 12 0 154 195 63 104 1.46e-11 63.9
MS.gene57861.t1 MTR_3g073560 62.500 56 21 0 143 198 50 105 1.54e-11 64.3
MS.gene57861.t1 MTR_8g091650 69.767 43 13 0 156 198 109 151 1.64e-11 63.9
MS.gene57861.t1 MTR_8g091650 68.293 41 13 0 158 198 2 42 5.52e-11 62.0
MS.gene57861.t1 MTR_4g053250 46.739 92 29 4 115 195 144 226 5.96e-11 62.8
MS.gene57861.t1 MTR_1g111310 50.000 60 30 0 141 200 404 463 7.05e-11 62.8
MS.gene57861.t1 MTR_1g111310 51.724 58 28 0 141 198 37 94 8.88e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57861.t1 AT5G67030 61.628 172 54 3 1 167 193 357 6.78e-63 207
MS.gene57861.t1 AT5G67030 61.628 172 54 3 1 167 193 357 1.39e-62 208
MS.gene57861.t1 AT5G65210 61.111 72 23 2 129 198 56 124 1.07e-16 79.3
MS.gene57861.t1 AT5G65210 61.111 72 23 2 129 198 56 124 1.07e-16 79.3
MS.gene57861.t1 AT5G65210 61.111 72 23 2 129 198 56 124 1.07e-16 79.3
MS.gene57861.t1 AT5G65210 61.111 72 23 2 129 198 56 124 1.07e-16 79.3
MS.gene57861.t1 AT5G65210 61.111 72 23 2 129 198 56 124 1.07e-16 79.3
MS.gene57861.t1 AT5G65210 61.111 72 23 2 129 198 56 124 1.07e-16 79.3
MS.gene57861.t1 AT5G10030 61.429 70 22 2 131 198 54 120 9.62e-16 76.3
MS.gene57861.t1 AT5G10030 61.429 70 22 2 131 198 54 120 9.62e-16 76.3
MS.gene57861.t1 AT5G10030 61.429 70 22 2 131 198 54 120 9.62e-16 76.3
MS.gene57861.t1 AT5G10030 61.429 70 22 2 131 198 54 120 9.62e-16 76.3
MS.gene57861.t1 AT5G10030 61.429 70 22 2 131 198 54 120 9.62e-16 76.3
MS.gene57861.t1 AT5G65210 73.077 52 12 1 149 198 3 54 6.75e-15 73.6
MS.gene57861.t1 AT1G77920 78.571 42 9 0 157 198 92 133 6.28e-13 68.2
MS.gene57861.t1 AT1G22070 47.436 78 35 1 128 199 62 139 1.26e-12 67.4
MS.gene57861.t1 AT1G08320 56.716 67 25 1 158 224 54 116 2.41e-12 66.6
MS.gene57861.t1 AT1G08320 56.716 67 25 1 158 224 54 116 2.41e-12 66.6
MS.gene57861.t1 AT1G08320 56.716 67 25 1 158 224 178 240 3.62e-12 66.2
MS.gene57861.t1 AT1G08320 56.716 67 25 1 158 224 178 240 3.62e-12 66.2
MS.gene57861.t1 AT1G08320 56.716 67 25 1 158 224 178 240 3.62e-12 66.2
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 36 86 4.18e-12 65.5
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 36 86 4.18e-12 65.5
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 36 86 4.18e-12 65.5
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 36 86 4.18e-12 65.5
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 61 111 4.85e-12 65.5
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 9 59 5.07e-12 65.1
MS.gene57861.t1 AT3G12250 66.667 51 17 0 148 198 30 80 5.38e-12 65.1
MS.gene57861.t1 AT5G06839 50.000 88 34 3 117 203 128 206 5.99e-12 65.5
MS.gene57861.t1 AT5G06950 66.667 51 17 0 148 198 36 86 7.52e-12 64.7
MS.gene57861.t1 AT5G06950 66.667 51 17 0 148 198 36 86 7.52e-12 64.7
MS.gene57861.t1 AT5G06950 66.667 51 17 0 148 198 36 86 7.52e-12 64.7
MS.gene57861.t1 AT5G06950 66.667 51 17 0 148 198 36 86 7.52e-12 64.7
MS.gene57861.t1 AT5G06950 66.667 51 17 0 148 198 36 86 7.52e-12 64.7
MS.gene57861.t1 AT5G06839 71.739 46 13 0 158 203 118 163 1.04e-11 64.7
MS.gene57861.t1 AT5G06960 78.049 41 9 0 158 198 46 86 1.06e-11 64.3
MS.gene57861.t1 AT5G06960 78.049 41 9 0 158 198 46 86 1.06e-11 64.3
MS.gene57861.t1 AT5G06839 71.739 46 13 0 158 203 118 163 1.06e-11 64.7
MS.gene57861.t1 AT5G06960 78.049 41 9 0 158 198 26 66 1.21e-11 64.3
MS.gene57861.t1 AT1G68640 76.316 38 9 0 161 198 169 206 7.12e-11 62.4

Find 59 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGGACAAACTTTGCTCTT+AGG 0.236085 5.2:+68379490 None:intergenic
AGTACCTTTGTGCAGGATTA+TGG 0.243323 5.2:-68379554 MS.gene57861:intron
CTGAAGATGTGAAGTATTAT+TGG 0.268807 5.2:-68380113 MS.gene57861:CDS
TAGGTGCGGACAAAGTTATT+TGG 0.277008 5.2:-68380489 MS.gene57861:CDS
AGGTGCGGACAAAGTTATTT+GGG 0.289347 5.2:-68380488 MS.gene57861:CDS
TGAAGGTACCGATTATTCTT+AGG 0.297944 5.2:-68380285 MS.gene57861:intron
AGAGGAGAGCAACTCTAATT+TGG 0.306680 5.2:-68379530 MS.gene57861:CDS
CAGGACAAACTTTGCTCTTA+GGG 0.322230 5.2:+68379491 None:intergenic
CATGTTCTTACCTTATCAAT+AGG 0.325874 5.2:+68380042 None:intergenic
GTCTGATAAACCAGTGTAAC+AGG 0.330006 5.2:+68380437 None:intergenic
TTGGAGGAGCAGATGGGATT+TGG 0.337889 5.2:-68381002 MS.gene57861:CDS
CAACACCTCCTGCAGGTTCC+TGG 0.339854 5.2:+68380199 None:intergenic
CATTTGGAGCTGTTGTCATT+TGG 0.345326 5.2:-68378372 MS.gene57861:CDS
GGCAACAAAAGCTACATATT+CGG 0.361215 5.2:-68380466 MS.gene57861:CDS
AGATTCTTGCCCGTGCAGTC+TGG 0.364988 5.2:-68381232 MS.gene57861:CDS
GCAACAAAAGCTACATATTC+GGG 0.407896 5.2:-68380465 MS.gene57861:CDS
GTTTGAGTCCTAAGAATAAT+CGG 0.408090 5.2:+68380277 None:intergenic
TACATGCAAGTGTTGTCATT+TGG 0.414200 5.2:-68378388 MS.gene57861:CDS
CTCTTGGTTGGAGGAGCAGA+TGG 0.447503 5.2:-68381009 MS.gene57861:CDS
TTTGTAGGTAACAGTGGTGT+TGG 0.447827 5.2:-68381058 MS.gene57861:intron
TTTCGTTGATCATGAAACTA+AGG 0.452979 5.2:-68381174 MS.gene57861:intron
GTATCTTCAGACGTCGGTGC+TGG 0.463892 5.2:-68380246 MS.gene57861:CDS
AGCTAATAAACCTATTGATA+AGG 0.470778 5.2:-68380052 MS.gene57861:intron
TATGATGGAGATCTCTTGGT+TGG 0.476463 5.2:-68381021 MS.gene57861:CDS
TCGTTTGAAGTTAGTGCAGT+TGG 0.476876 5.2:-68379740 MS.gene57861:CDS
CACCCAACCGATTCAATGTC+AGG 0.483163 5.2:+68380405 None:intergenic
AGTTAGTGCAGTTGGACAGC+AGG 0.487464 5.2:-68379732 MS.gene57861:intron
GAAGTATGATGGAGATCTCT+TGG 0.487741 5.2:-68381025 MS.gene57861:CDS
GCTTTAAGAATATCAGCAGC+AGG 0.488568 5.2:-68378480 MS.gene57861:intron
GTAACAGTGGTGTTGGATAA+TGG 0.488993 5.2:-68381051 MS.gene57861:CDS
GATTCTTGCCCGTGCAGTCT+GGG 0.493844 5.2:-68381231 MS.gene57861:CDS
GATAATGGTCAGAAGTATGA+TGG 0.499455 5.2:-68381036 MS.gene57861:CDS
TCGGGCTATACCTGTTACAC+TGG 0.500901 5.2:-68380447 MS.gene57861:CDS
CTGCACGGGCAAGAATCTCT+TGG 0.506850 5.2:+68381237 None:intergenic
TTTGTGCAGGATTATGGAAG+AGG 0.521606 5.2:-68379548 MS.gene57861:intron
CGACCTGACATTGAATCGGT+TGG 0.526755 5.2:-68380408 MS.gene57861:CDS
ATGTTCACTCTAACAGCAAA+GGG 0.529692 5.2:-68378417 MS.gene57861:CDS
TGTGCGACCTGACATTGAAT+CGG 0.531421 5.2:-68380412 MS.gene57861:CDS
GGGGTATCAACACCTCCTGC+AGG 0.542175 5.2:+68380192 None:intergenic
AGCAGATGGGATTTGGTCAA+AGG 0.542351 5.2:-68380995 MS.gene57861:intron
GATGGAGATCTCTTGGTTGG+AGG 0.542419 5.2:-68381018 MS.gene57861:CDS
GACCTGACATTGAATCGGTT+GGG 0.548057 5.2:-68380407 MS.gene57861:intron
GCATTTCACCAGGAACCTGC+AGG 0.552426 5.2:-68380207 MS.gene57861:CDS
AATGTTCACTCTAACAGCAA+AGG 0.575318 5.2:-68378418 MS.gene57861:CDS
TGACATCATCCCAGACTGCA+CGG 0.575551 5.2:+68381222 None:intergenic
TGGGATGATGTCATTATGAA+TGG 0.583211 5.2:-68381212 MS.gene57861:CDS
GACATCATCCCAGACTGCAC+GGG 0.598139 5.2:+68381223 None:intergenic
GGTGCGGACAAAGTTATTTG+GGG 0.605778 5.2:-68380487 MS.gene57861:CDS
TCTTGGTTGGAGGAGCAGAT+GGG 0.610379 5.2:-68381008 MS.gene57861:CDS
ATAATACTTCACATCTTCAG+AGG 0.611011 5.2:+68380116 None:intergenic
TTTAGAAGCACAAACCATTG+GGG 0.619689 5.2:+68380172 None:intergenic
GATTGAATATGCAGATACAA+AGG 0.645585 5.2:-68379933 MS.gene57861:intron
TACTTTGTATCTTCAGACGT+CGG 0.646666 5.2:-68380252 MS.gene57861:CDS
TCGGTGCTGGAAAGATGCAA+TGG 0.648668 5.2:-68380233 MS.gene57861:CDS
ATATCATTTGTAGGTAACAG+TGG 0.652755 5.2:-68381064 MS.gene57861:intron
TTAGAAGCACAAACCATTGG+GGG 0.656786 5.2:+68380173 None:intergenic
AGGACAAACTTTGCTCTTAG+GGG 0.667666 5.2:+68379492 None:intergenic
GCAATGGTATGCATTTCACC+AGG 0.671096 5.2:-68380217 MS.gene57861:CDS
TTTCACCAGGAACCTGCAGG+AGG 0.680945 5.2:-68380204 MS.gene57861:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTATTAAAAAGTATTTATA+TGG + chr5.2:68380998-68381017 None:intergenic 10.0%
!! ATTTAAACATGCATTAAAAT+TGG - chr5.2:68380006-68380025 MS.gene57861:intron 15.0%
!!! AATTTTCTATATAGTAACTT+AGG - chr5.2:68378660-68378679 MS.gene57861:intron 15.0%
!!! ATTTTCTATATAGTAACTTA+GGG - chr5.2:68378661-68378680 MS.gene57861:intron 15.0%
!!! GTATAATATAGAAATTTTAG+AGG - chr5.2:68380215-68380234 MS.gene57861:CDS 15.0%
!! AAAAAGATTATTCAACAGTT+AGG - chr5.2:68380181-68380200 MS.gene57861:intron 20.0%
!! AATGTATATGCCTATAAATA+TGG - chr5.2:68381084-68381103 MS.gene57861:intron 20.0%
!! ACATTGCTAATAATTAACAT+AGG - chr5.2:68378488-68378507 MS.gene57861:intron 20.0%
!! ATAAAATGCATGACTAAATT+GGG + chr5.2:68380935-68380954 None:intergenic 20.0%
!! ATATTTGGTATATCATTTGT+AGG - chr5.2:68378532-68378551 MS.gene57861:intron 20.0%
!! ATCAAAATGAAAAAATAAGC+AGG + chr5.2:68380560-68380579 None:intergenic 20.0%
!! ATTAACAATCAGATTGATAT+TGG + chr5.2:68381020-68381039 None:intergenic 20.0%
!! TATAAAATGCATGACTAAAT+TGG + chr5.2:68380936-68380955 None:intergenic 20.0%
!! TGTTAATTATTAGCAATGTA+AGG + chr5.2:68378487-68378506 None:intergenic 20.0%
!!! ACAATTACTTGAGATTTTTT+AGG + chr5.2:68378864-68378883 None:intergenic 20.0%
!!! CAATTACTTGAGATTTTTTA+GGG + chr5.2:68378863-68378882 None:intergenic 20.0%
!!! CATTGAGTTCATTTTATTTA+TGG - chr5.2:68380420-68380439 MS.gene57861:CDS 20.0%
!!! TGAAGCTTTTAAACAATATT+TGG - chr5.2:68378517-68378536 MS.gene57861:intron 20.0%
!!! TTTTCTATATAGTAACTTAG+GGG - chr5.2:68378662-68378681 MS.gene57861:intron 20.0%
! AACATGACATGATAAAATGA+TGG + chr5.2:68379274-68379293 None:intergenic 25.0%
! AATTACATCCATATTGTTAG+AGG + chr5.2:68380682-68380701 None:intergenic 25.0%
! AGCTAATAAACCTATTGATA+AGG - chr5.2:68379553-68379572 MS.gene57861:intron 25.0%
! ATGAGAAAAAGGAATTTGAA+TGG + chr5.2:68380832-68380851 None:intergenic 25.0%
! ATTAGAAAATGACCTGTAAT+TGG + chr5.2:68379059-68379078 None:intergenic 25.0%
! CATTGAATTATTCTGTGAAT+AGG - chr5.2:68379097-68379116 MS.gene57861:intron 25.0%
! GGAATTAACAAAACTGTTTA+TGG - chr5.2:68378923-68378942 MS.gene57861:intron 25.0%
! TTACTCTATGAATAAGTTTC+CGG + chr5.2:68380144-68380163 None:intergenic 25.0%
!! AAGTTAAAATGTCATTTGAG+TGG - chr5.2:68380768-68380787 MS.gene57861:intron 25.0%
!! AGTTAAAATGTCATTTGAGT+GGG - chr5.2:68380769-68380788 MS.gene57861:intron 25.0%
!! CTTGTGTCTTGATTTTATAA+TGG + chr5.2:68379522-68379541 None:intergenic 25.0%
!! GCAATTCTAATTTTGCAATA+TGG - chr5.2:68380710-68380729 MS.gene57861:intron 25.0%
!!! ATAGTTCTGAATTGTGATTA+CGG + chr5.2:68380322-68380341 None:intergenic 25.0%
!!! CATTTGTGATTTTGAAGAAA+AGG - chr5.2:68381164-68381183 MS.gene57861:intron 25.0%
!!! CATTTTGATACTTGATATCT+TGG - chr5.2:68380571-68380590 MS.gene57861:intron 25.0%
AACACAAAATGTTGTATCAG+AGG - chr5.2:68378902-68378921 MS.gene57861:intron 30.0%
AATATGCAGATTGATGTATG+AGG - chr5.2:68380362-68380381 MS.gene57861:intron 30.0%
AATTATTCTGTGAATAGGTG+CGG - chr5.2:68379102-68379121 MS.gene57861:intron 30.0%
ACATCCAAAATTTGAAAGCA+TGG + chr5.2:68379629-68379648 None:intergenic 30.0%
AGGGATTAAAGTTAGAGAAA+TGG + chr5.2:68379232-68379251 None:intergenic 30.0%
ATAATACTTCACATCTTCAG+AGG + chr5.2:68379492-68379511 None:intergenic 30.0%
ATATCATTTGTAGGTAACAG+TGG - chr5.2:68378541-68378560 MS.gene57861:intron 30.0%
ATTTAGGGAAGCTTCAATAA+AGG + chr5.2:68378736-68378755 None:intergenic 30.0%
CATGTTCTTACCTTATCAAT+AGG + chr5.2:68379566-68379585 None:intergenic 30.0%
GATTGAATATGCAGATACAA+AGG - chr5.2:68379672-68379691 MS.gene57861:intron 30.0%
GTAATCACAATTCAGAACTA+TGG - chr5.2:68380321-68380340 MS.gene57861:intron 30.0%
GTCATTGAAGCCATATTTAT+AGG + chr5.2:68381097-68381116 None:intergenic 30.0%
GTCCAAAACCTTACAATTTA+GGG + chr5.2:68378751-68378770 None:intergenic 30.0%
GTTTGAGTCCTAAGAATAAT+CGG + chr5.2:68379331-68379350 None:intergenic 30.0%
TGTCCAAAACCTTACAATTT+AGG + chr5.2:68378752-68378771 None:intergenic 30.0%
TTCAATAAAGGAGTAAAGCA+AGG + chr5.2:68378724-68378743 None:intergenic 30.0%
TTTCGTTGATCATGAAACTA+AGG - chr5.2:68378431-68378450 MS.gene57861:CDS 30.0%
TTTGGACATTAAAACAAGGT+GGG - chr5.2:68378764-68378783 MS.gene57861:intron 30.0%
! CCAGGAAGTATAGTTAAATA+GGG + chr5.2:68379251-68379270 None:intergenic 30.0%
! CTGAAGATGTGAAGTATTAT+TGG - chr5.2:68379492-68379511 MS.gene57861:CDS 30.0%
! GATACCATGCTTTCAAATTT+TGG - chr5.2:68379622-68379641 MS.gene57861:intron 30.0%
! GTTAAAATGTCATTTGAGTG+GGG - chr5.2:68380770-68380789 MS.gene57861:intron 30.0%
! TTCCCTAAATTGTAAGGTTT+TGG - chr5.2:68378746-68378765 MS.gene57861:intron 30.0%
! TTTTGGACATTAAAACAAGG+TGG - chr5.2:68378763-68378782 MS.gene57861:intron 30.0%
!! ACTGTGATTTTGACACAATT+TGG - chr5.2:68379933-68379952 MS.gene57861:intron 30.0%
!! ATGTGTTATTTACACATGAG+AGG + chr5.2:68380899-68380918 None:intergenic 30.0%
!! TGTGTTATTTACACATGAGA+GGG + chr5.2:68380898-68380917 None:intergenic 30.0%
!! TTGCAGGTTTATTGGTTTAT+AGG - chr5.2:68381051-68381070 MS.gene57861:CDS 30.0%
!!! AATTGCGATTTTAGCTGTAT+TGG - chr5.2:68380256-68380275 MS.gene57861:CDS 30.0%
!!! AGGTTTTGGACATTAAAACA+AGG - chr5.2:68378760-68378779 MS.gene57861:intron 30.0%
!!! TTAAAACCTTTTTTCGCAGA+CGG + chr5.2:68379733-68379752 None:intergenic 30.0%
AAAGGAGTAAAGCAAGGTTT+GGG + chr5.2:68378718-68378737 None:intergenic 35.0%
AACCAATAAACCTGCAAGAT+AGG + chr5.2:68381048-68381067 None:intergenic 35.0%
AATGTTCACTCTAACAGCAA+AGG - chr5.2:68381187-68381206 MS.gene57861:CDS 35.0%
ACATCCATATTGTTAGAGGA+AGG + chr5.2:68380678-68380697 None:intergenic 35.0%
ACATTGCGGACAATTTATCA+CGG + chr5.2:68380292-68380311 None:intergenic 35.0%
ACTTGAACTTCTTTGGTTGA+AGG - chr5.2:68379303-68379322 MS.gene57861:intron 35.0%
AGAAAATGACCTGTAATTGG+AGG + chr5.2:68379056-68379075 None:intergenic 35.0%
ATCCTATCTTGCAGGTTTAT+TGG - chr5.2:68381043-68381062 MS.gene57861:CDS 35.0%
ATGTTCACTCTAACAGCAAA+GGG - chr5.2:68381188-68381207 MS.gene57861:CDS 35.0%
CATATTGTTAGAGGAAGGTT+AGG + chr5.2:68380673-68380692 None:intergenic 35.0%
CCCTATTTAACTATACTTCC+TGG - chr5.2:68379248-68379267 MS.gene57861:intron 35.0%
CTAACCTTCCTCTAACAATA+TGG - chr5.2:68380671-68380690 MS.gene57861:intron 35.0%
GAGAGAAACACATGAGAAAA+AGG + chr5.2:68380843-68380862 None:intergenic 35.0%
GCAACAAAAGCTACATATTC+GGG - chr5.2:68379140-68379159 MS.gene57861:intron 35.0%
GGCAACAAAAGCTACATATT+CGG - chr5.2:68379139-68379158 MS.gene57861:intron 35.0%
TAAAGGAGTAAAGCAAGGTT+TGG + chr5.2:68378719-68378738 None:intergenic 35.0%
TACTTTGTATCTTCAGACGT+CGG - chr5.2:68379353-68379372 MS.gene57861:intron 35.0%
TGAAGCTTCCCTAAATTGTA+AGG - chr5.2:68378740-68378759 MS.gene57861:intron 35.0%
TGGGATGATGTCATTATGAA+TGG - chr5.2:68378393-68378412 MS.gene57861:CDS 35.0%
TGTTTCTCTCTCTTGATGAA+TGG - chr5.2:68380853-68380872 MS.gene57861:intron 35.0%
TTAATCACATCCTATCTTGC+AGG - chr5.2:68381035-68381054 MS.gene57861:CDS 35.0%
! ACATGTGCTATTGATTATGC+TGG + chr5.2:68379005-68379024 None:intergenic 35.0%
! AGTTTAGAAGCACAAACCAT+TGG + chr5.2:68379438-68379457 None:intergenic 35.0%
! CATGTGCTATTGATTATGCT+GGG + chr5.2:68379004-68379023 None:intergenic 35.0%
! GATAATGGTCAGAAGTATGA+TGG - chr5.2:68378569-68378588 MS.gene57861:intron 35.0%
! GCCAGGAAGTATAGTTAAAT+AGG + chr5.2:68379252-68379271 None:intergenic 35.0%
! GTTTAGAAGCACAAACCATT+GGG + chr5.2:68379437-68379456 None:intergenic 35.0%
! TAATTAGAGTACCTTTGTGC+AGG - chr5.2:68380044-68380063 MS.gene57861:intron 35.0%
! TACATGCAAGTGTTGTCATT+TGG - chr5.2:68381217-68381236 MS.gene57861:CDS 35.0%
! TTTAGAAGCACAAACCATTG+GGG + chr5.2:68379436-68379455 None:intergenic 35.0%
!! AGCTTTATTCCTCCAATTAC+AGG - chr5.2:68379044-68379063 MS.gene57861:intron 35.0%
!! GACTAGTCTTTCAAGTTTAG+AGG + chr5.2:68380476-68380495 None:intergenic 35.0%
!! TGAAGGTACCGATTATTCTT+AGG - chr5.2:68379320-68379339 MS.gene57861:intron 35.0%
AAATTGTCCGCAATGTCACT+AGG - chr5.2:68380296-68380315 MS.gene57861:intron 40.0%
AACTTAGGGGAAGAGTTTGA+AGG - chr5.2:68378675-68378694 MS.gene57861:intron 40.0%
AAGTGTACACAAAGTGGCTT+AGG + chr5.2:68380973-68380992 None:intergenic 40.0%
ACAGGACAAACTTTGCTCTT+AGG + chr5.2:68380118-68380137 None:intergenic 40.0%
AGAGGAGAGCAACTCTAATT+TGG - chr5.2:68380075-68380094 MS.gene57861:CDS 40.0%
AGGACAAACTTTGCTCTTAG+GGG + chr5.2:68380116-68380135 None:intergenic 40.0%
AGGTGCGGACAAAGTTATTT+GGG - chr5.2:68379117-68379136 MS.gene57861:intron 40.0%
AGTACCTTTGTGCAGGATTA+TGG - chr5.2:68380051-68380070 MS.gene57861:intron 40.0%
ATGGCTAAGTGTACACAAAG+TGG + chr5.2:68380979-68380998 None:intergenic 40.0%
CAGCCAAAAATGCAGAAACA+GGG + chr5.2:68380603-68380622 None:intergenic 40.0%
CAGGACAAACTTTGCTCTTA+GGG + chr5.2:68380117-68380136 None:intergenic 40.0%
CATTTCTGACATTGCCTTAC+AGG - chr5.2:68379817-68379836 MS.gene57861:intron 40.0%
GACATGATAAAATGATGGCC+AGG + chr5.2:68379269-68379288 None:intergenic 40.0%
GTCTGATAAACCAGTGTAAC+AGG + chr5.2:68379171-68379190 None:intergenic 40.0%
TAGGTGCGGACAAAGTTATT+TGG - chr5.2:68379116-68379135 MS.gene57861:intron 40.0%
TCTTCCATAATCCTGCACAA+AGG + chr5.2:68380058-68380077 None:intergenic 40.0%
TGAATAAGTTTCCGGCATAC+AGG + chr5.2:68380136-68380155 None:intergenic 40.0%
TTTGTAGGTAACAGTGGTGT+TGG - chr5.2:68378547-68378566 MS.gene57861:intron 40.0%
TTTGTGCAGGATTATGGAAG+AGG - chr5.2:68380057-68380076 MS.gene57861:CDS 40.0%
! CATTTGGAGCTGTTGTCATT+TGG - chr5.2:68381233-68381252 MS.gene57861:CDS 40.0%
! GAAGTATGATGGAGATCTCT+TGG - chr5.2:68378580-68378599 MS.gene57861:intron 40.0%
! GTAACAGTGGTGTTGGATAA+TGG - chr5.2:68378554-68378573 MS.gene57861:intron 40.0%
! TATGATGGAGATCTCTTGGT+TGG - chr5.2:68378584-68378603 MS.gene57861:intron 40.0%
! TCGTTTGAAGTTAGTGCAGT+TGG - chr5.2:68379865-68379884 MS.gene57861:intron 40.0%
! TTAGAAGCACAAACCATTGG+GGG + chr5.2:68379435-68379454 None:intergenic 40.0%
!! GAAGCTGACTTGAACTTCTT+TGG - chr5.2:68379296-68379315 MS.gene57861:intron 40.0%
!! GCTTTAAGAATATCAGCAGC+AGG - chr5.2:68381125-68381144 MS.gene57861:intron 40.0%
!! TAGAGGTCTCTAAAACTGCA+CGG - chr5.2:68380232-68380251 MS.gene57861:CDS 40.0%
!!! TCACCCTGTTTCTGCATTTT+TGG - chr5.2:68380597-68380616 MS.gene57861:intron 40.0%
AATGCGCAACTTTGTACAGG+AGG + chr5.2:68378823-68378842 None:intergenic 45.0%
ACATGAGAGGGTAAAGTGTG+TGG + chr5.2:68380886-68380905 None:intergenic 45.0%
ATGCGCAACTTTGTACAGGA+GGG + chr5.2:68378822-68378841 None:intergenic 45.0%
ATTACGGCCTAGTGACATTG+CGG + chr5.2:68380306-68380325 None:intergenic 45.0%
ATTGAATCGGTTGGGTGAGT+TGG - chr5.2:68379206-68379225 MS.gene57861:intron 45.0%
GACCTGACATTGAATCGGTT+GGG - chr5.2:68379198-68379217 MS.gene57861:intron 45.0%
GCAAAGTTTGTCCTGTATGC+CGG - chr5.2:68380122-68380141 MS.gene57861:intron 45.0%
GCAATGGTATGCATTTCACC+AGG - chr5.2:68379388-68379407 MS.gene57861:intron 45.0%
GCAGCCAAAAATGCAGAAAC+AGG + chr5.2:68380604-68380623 None:intergenic 45.0%
GGTGCGGACAAAGTTATTTG+GGG - chr5.2:68379118-68379137 MS.gene57861:intron 45.0%
TAAGAGCCGTCTGCGAAAAA+AGG - chr5.2:68379724-68379743 MS.gene57861:intron 45.0%
TGTGCGACCTGACATTGAAT+CGG - chr5.2:68379193-68379212 MS.gene57861:intron 45.0%
TTAGGGAGCCTAGAGAGAAA+GGG + chr5.2:68378846-68378865 None:intergenic 45.0%
TTTAGGGAGCCTAGAGAGAA+AGG + chr5.2:68378847-68378866 None:intergenic 45.0%
! AGAGGAAGGTTAGGCTTTGT+TGG + chr5.2:68380664-68380683 None:intergenic 45.0%
! AGCAGATGGGATTTGGTCAA+AGG - chr5.2:68378610-68378629 MS.gene57861:intron 45.0%
AACTTTGTACAGGAGGGTGG+GGG + chr5.2:68378816-68378835 None:intergenic 50.0%
AGTTAGTGCAGTTGGACAGC+AGG - chr5.2:68379873-68379892 MS.gene57861:intron 50.0%
CAACTTTGTACAGGAGGGTG+GGG + chr5.2:68378817-68378836 None:intergenic 50.0%
CGACCTGACATTGAATCGGT+TGG - chr5.2:68379197-68379216 MS.gene57861:intron 50.0%
CTGCTGCACATAAGCCTGTA+AGG + chr5.2:68379834-68379853 None:intergenic 50.0%
GATGGAGATCTCTTGGTTGG+AGG - chr5.2:68378587-68378606 MS.gene57861:intron 50.0%
GCAACTTTGTACAGGAGGGT+GGG + chr5.2:68378818-68378837 None:intergenic 50.0%
GGGAATGCGCAACTTTGTAC+AGG + chr5.2:68378826-68378845 None:intergenic 50.0%
TCGGGCTATACCTGTTACAC+TGG - chr5.2:68379158-68379177 MS.gene57861:intron 50.0%
TCTTGGTTGGAGGAGCAGAT+GGG - chr5.2:68378597-68378616 MS.gene57861:intron 50.0%
TGACATCATCCCAGACTGCA+CGG + chr5.2:68378386-68378405 None:intergenic 50.0%
TGCGCATTCCCTTTCTCTCT+AGG - chr5.2:68378835-68378854 MS.gene57861:intron 50.0%
TTGGAGGAGCAGATGGGATT+TGG - chr5.2:68378603-68378622 MS.gene57861:intron 50.0%
! CACCCAACCGATTCAATGTC+AGG + chr5.2:68379203-68379222 None:intergenic 50.0%
!! TCGGTGCTGGAAAGATGCAA+TGG - chr5.2:68379372-68379391 MS.gene57861:intron 50.0%
ACTTTGTACAGGAGGGTGGG+GGG + chr5.2:68378815-68378834 None:intergenic 55.0%
AGATTCTTGCCCGTGCAGTC+TGG - chr5.2:68378373-68378392 MS.gene57861:CDS 55.0%
CGCAACTTTGTACAGGAGGG+TGG + chr5.2:68378819-68378838 None:intergenic 55.0%
CTCTTGGTTGGAGGAGCAGA+TGG - chr5.2:68378596-68378615 MS.gene57861:intron 55.0%
CTGCACGGGCAAGAATCTCT+TGG + chr5.2:68378371-68378390 None:intergenic 55.0%
GACATCATCCCAGACTGCAC+GGG + chr5.2:68378385-68378404 None:intergenic 55.0%
GATTCTTGCCCGTGCAGTCT+GGG - chr5.2:68378374-68378393 MS.gene57861:CDS 55.0%
GCATTTCACCAGGAACCTGC+AGG - chr5.2:68379398-68379417 MS.gene57861:intron 55.0%
GTATCTTCAGACGTCGGTGC+TGG - chr5.2:68379359-68379378 MS.gene57861:intron 55.0%
TGCAGTTGGACAGCAGGTGT+GGG - chr5.2:68379879-68379898 MS.gene57861:intron 55.0%
TTTCACCAGGAACCTGCAGG+AGG - chr5.2:68379401-68379420 MS.gene57861:intron 55.0%
!! GGAGGTGTTGATACCCCCAA+TGG - chr5.2:68379419-68379438 MS.gene57861:intron 55.0%
CAACACCTCCTGCAGGTTCC+TGG + chr5.2:68379409-68379428 None:intergenic 60.0%
GCAGTTGGACAGCAGGTGTG+GGG - chr5.2:68379880-68379899 MS.gene57861:intron 60.0%
GTGCAGTTGGACAGCAGGTG+TGG - chr5.2:68379878-68379897 MS.gene57861:intron 60.0%
! GGGGTATCAACACCTCCTGC+AGG + chr5.2:68379416-68379435 None:intergenic 60.0%
GTGGGGGGAGGGAGGAGAAA+AGG + chr5.2:68378800-68378819 None:intergenic 65.0%
TGTACAGGAGGGTGGGGGGA+GGG + chr5.2:68378811-68378830 None:intergenic 65.0%
TTGTACAGGAGGGTGGGGGG+AGG + chr5.2:68378812-68378831 None:intergenic 65.0%
ACAGGAGGGTGGGGGGAGGG+AGG + chr5.2:68378808-68378827 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 68378360 68381267 68378360 ID=MS.gene57861
chr5.2 mRNA 68378360 68381267 68378360 ID=MS.gene57861.t1;Parent=MS.gene57861
chr5.2 exon 68381175 68381267 68381175 ID=MS.gene57861.t1.exon1;Parent=MS.gene57861.t1
chr5.2 CDS 68381175 68381267 68381175 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68380996 68381073 68380996 ID=MS.gene57861.t1.exon2;Parent=MS.gene57861.t1
chr5.2 CDS 68380996 68381073 68380996 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68380408 68380508 68380408 ID=MS.gene57861.t1.exon3;Parent=MS.gene57861.t1
chr5.2 CDS 68380408 68380508 68380408 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68380187 68380302 68380187 ID=MS.gene57861.t1.exon4;Parent=MS.gene57861.t1
chr5.2 CDS 68380187 68380302 68380187 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68380053 68380138 68380053 ID=MS.gene57861.t1.exon5;Parent=MS.gene57861.t1
chr5.2 CDS 68380053 68380138 68380053 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68379882 68379941 68379882 ID=MS.gene57861.t1.exon6;Parent=MS.gene57861.t1
chr5.2 CDS 68379882 68379941 68379882 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68379733 68379788 68379733 ID=MS.gene57861.t1.exon7;Parent=MS.gene57861.t1
chr5.2 CDS 68379733 68379788 68379733 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68379492 68379561 68379492 ID=MS.gene57861.t1.exon8;Parent=MS.gene57861.t1
chr5.2 CDS 68379492 68379561 68379492 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
chr5.2 exon 68378360 68378494 68378360 ID=MS.gene57861.t1.exon9;Parent=MS.gene57861.t1
chr5.2 CDS 68378360 68378494 68378360 ID=cds.MS.gene57861.t1;Parent=MS.gene57861.t1
Gene Sequence

>MS.gene57861

ATGGCTTTCCAAGAGATTCTTGCCCGTGCAGTCTGGGATGATGTCATTATGAATGGTAGTAAAGTTGTCGATTTCGTTGATCATGAAACTAAGGTAATCAAAGAAAGCTTTTATTGAAGATTCTCCTTACATTGCTAATAATTAACATAGGTATTTTTGAAGCTTTTAAACAATATTTGGTATATCATTTGTAGGTAACAGTGGTGTTGGATAATGGTCAGAAGTATGATGGAGATCTCTTGGTTGGAGGAGCAGATGGGATTTGGTCAAAGGTCTTCTTAAATTCTTTCTTTTATTCATAATTTTCTATATAGTAACTTAGGGGAAGAGTTTGAAGGAAATTTTAAAATACTCTCCCAAACCTTGCTTTACTCCTTTATTGAAGCTTCCCTAAATTGTAAGGTTTTGGACATTAAAACAAGGTGGGAGCTTATCATCCTTTTCTCCTCCCTCCCCCCACCCTCCTGTACAAAGTTGCGCATTCCCTTTCTCTCTAGGCTCCCTAAAAAATCTCAAGTAATTGTATACTCAGCATAATCAATAACACAAAATGTTGTATCAGAGGAATTAACAAAACTGTTTATGGTATAGATATCTTCTAGAAAAGTTAGTCTTTTATACTTGAAAACACATAATGTATACCCAGCATAATCAATAGCACATGTGTATGTAAATTTATTTTTCAGCTTTATTCCTCCAATTACAGGTCATTTTCTAATTTAATGTCTTCATTATATCATTGAATTATTCTGTGAATAGGTGCGGACAAAGTTATTTGGGGCAACAAAAGCTACATATTCGGGCTATACCTGTTACACTGGTTTATCAGACTTTGTGCGACCTGACATTGAATCGGTTGGGTGAGTTGGCCATTTCTCTAACTTTAATCCCTATTTAACTATACTTCCTGGCCATCATTTTATCATGTCATGTTGAGAAGCTGACTTGAACTTCTTTGGTTGAAGGTACCGATTATTCTTAGGACTCAAACAATACTTTGTATCTTCAGACGTCGGTGCTGGAAAGATGCAATGGTATGCATTTCACCAGGAACCTGCAGGAGGTGTTGATACCCCCAATGGTTTGTGCTTCTAAACTAAATGTATGTGTAACATCTGTCAGAGAATAGCCTCTGAAGATGTGAAGTATTATTGGCACTCCATTATAAAATCAAGACACAAGTCTGAGCAAGAAGCTAATAAACCTATTGATAAGGTAAGAACATGAAAAATCTGTATTAAGTGCACATTAAGTCATTTACTGATACCATGCTTTCAAATTTTGGATGTTGTACATTAAGTCATTCTGATATGATTGAATATGCAGATACAAAGGCGTCTCGCACATAATCGAGAAGCTGCACGTAAGAGCCGTCTGCGAAAAAAGGTTTTAATGTTGTTTACAAATTGATGAATATGTCAGACGAGTAATTATTAGTGTCAATGTACTGATCAAGTCATTTCTGACATTGCCTTACAGGCTTATGTGCAGCAGCTAGAATCGAGTCGTTTGAAGTTAGTGCAGTTGGACAGCAGGTGTGGGGAAGATGAGTTATTATTTTACTAAACACGACACTGTGATTTTGACACAATTTGGAACTTCAGTGAAATGATAGTGTCACATTGATTTTACTAAACTCGATGTCAATTTAAACATGCATTAAAATTGGAATTATATGAGATTCTAATTAGAGTACCTTTGTGCAGGATTATGGAAGAGGAGAGCAACTCTAATTTGGCGAATCAAAGCACTACCCCTAAGAGCAAAGTTTGTCCTGTATGCCGGAAACTTATTCATAGAGTAAAAAGATTATTCTAGAGTAAAAAGATTATTCAACAGTTAGGTTCATACTGCTGTATAATATAGAAATTTTAGAGGTCTCTAAAACTGCACGGTAATTGCGATTTTAGCTGTATTGGATGTATTTTGCCGTGATAAATTGTCCGCAATGTCACTAGGCCGTAATCACAATTCAGAACTATGGTTACTGAATATGCTCACAAATATGCAGATTGATGTATGAGGTTTACATATAGTCAATAATTAGATTATGATTATGACATTGAGTTCATTTTATTTATGGCAGCAAGCAATATATGTCAATATTTTGTCACCTCTAAACTTGAAAGACTAGTCTTAGAAAATTGTCTTCTACTTTTGACAGTACAATTAGCACTTTGTTTTAGAATTTGTTGATCCTGCTTATTTTTTCATTTTGATACTTGATATCTTGGTAATCACCCTGTTTCTGCATTTTTGGCTGCAAATTTTGCAATTCTAATTTGACAAATTATGTTGTTTCCAACAAAGCCTAACCTTCCTCTAACAATATGGATGTAATTCAAATTTTGCAATTCTAATTTTGCAATATGGATGTACATGTTTGACACAATAGTTCATTTACAGTTAAGTTAAAATGTCATTTGAGTGGGGAATCATGCTTCTGAGAGATTAAAGTGTGATATATCACCATTCAAATTCCTTTTTCTCATGTGTTTCTCTCTCTTGATGAATGGCGATAGACCACACACTTTACCCTCTCATGTGTAAATAACACATGAGAGAATTCATTCCCAATTTAGTCATGCATTTTATAGTCATGAGCATATTCCTAAGCCACTTTGTGTACACTTAGCCATATAAATACTTTTTAATAACCAATATCAATCTGATTGTTAATCACATCCTATCTTGCAGGTTTATTGGTTTATAGGCATATTATTCAATGTATATGCCTATAAATATGGCTTCAATGACATGATTTTGCTTTAAGAATATCAGCAGCAGGATCGACAAGTTTTCGACATTTGTGATTTTGAAGAAAAGGAATGTTCACTCTAACAGCAAAGGGATGTCATACATGCAAGTGTTGTCATTTGGAGCTGTTGTCATTTGGAGCAGCATTTGA
Protein sequence

>MS.gene57861.t1

MAFQEILARAVWDDVIMNGSKVVDFVDHETKVTVVLDNGQKYDGDLLVGGADGIWSKVRTKLFGATKATYSGYTCYTGLSDFVRPDIESVGYRLFLGLKQYFVSSDVGAGKMQWYAFHQEPAGGVDTPNASEDVKYYWHSIIKSRHKSEQEANKPIDKIQRRLAHNREAARKSRLRKKAYVQQLESSRLKLVQLDSRIMEEESNSNLANQSTTPKSKVCPNISSRIDKFSTFVILKKRNVHSNSKGMSYMQVLSFGAVVIWSSI