AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58422

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58422.t1 MTR_4g108720 96.910 356 11 0 1 356 1 356 0.0 715
MS.gene58422.t1 MTR_5g041650 49.167 360 163 8 1 351 38 386 2.32e-107 320
MS.gene58422.t1 MTR_3g102570 36.872 179 102 5 1 173 1 174 1.37e-28 113
MS.gene58422.t1 MTR_4g084740 31.752 274 152 10 1 251 1 262 3.16e-26 107
MS.gene58422.t1 MTR_5g066180 34.302 172 79 5 1 169 1 141 7.43e-18 82.0
MS.gene58422.t1 MTR_1g029670 39.423 104 63 0 1 104 1 104 5.70e-17 78.2
MS.gene58422.t1 MTR_3g088615 62.264 53 20 0 1 53 1 53 3.24e-16 76.3
MS.gene58422.t1 MTR_1g053070 48.611 72 35 1 1 72 1 70 6.63e-16 77.0
MS.gene58422.t1 MTR_5g032520 48.571 70 34 1 1 70 1 68 7.81e-15 73.6
MS.gene58422.t1 MTR_5g032150 48.571 70 34 1 1 70 1 68 8.50e-15 73.2
MS.gene58422.t1 MTR_0003s0590 46.479 71 36 1 1 71 1 69 9.12e-15 73.6
MS.gene58422.t1 MTR_5g046790 45.570 79 42 1 1 79 1 78 1.07e-14 72.8
MS.gene58422.t1 MTR_2g017865 45.070 71 37 1 1 71 16 84 1.19e-14 73.6
MS.gene58422.t1 MTR_3g113030 56.604 53 23 0 1 53 1 53 1.28e-14 72.8
MS.gene58422.t1 MTR_8g066260 43.038 79 44 1 1 79 1 78 1.79e-14 72.8
MS.gene58422.t1 MTR_2g009890 46.479 71 36 1 1 71 1 69 1.82e-14 73.2
MS.gene58422.t1 MTR_5g066960 50.943 53 26 0 1 53 1 53 1.84e-14 67.8
MS.gene58422.t1 MTR_1g038300 45.070 71 39 0 1 71 1 71 2.16e-14 72.4
MS.gene58422.t1 MTR_7g075870 30.769 182 96 5 1 180 1 154 2.32e-14 71.6
MS.gene58422.t1 MTR_3g452380 54.717 53 24 0 1 53 16 68 2.93e-14 72.0
MS.gene58422.t1 MTR_4g109830 43.038 79 44 1 1 79 1 78 3.06e-14 71.6
MS.gene58422.t1 MTR_3g005530 54.717 53 24 0 1 53 1 53 3.55e-14 71.2
MS.gene58422.t1 MTR_2g461710 54.717 53 24 0 1 53 1 53 3.78e-14 67.0
MS.gene58422.t1 MTR_4g093970 48.571 70 34 1 1 70 27 94 5.04e-14 71.2
MS.gene58422.t1 MTR_5g031000 43.662 71 38 1 1 71 1 69 7.78e-14 70.5
MS.gene58422.t1 MTR_1g101970 46.575 73 37 1 1 73 1 71 8.91e-14 69.7
MS.gene58422.t1 MTR_8g087860 52.830 53 25 0 1 53 17 69 9.01e-14 70.5
MS.gene58422.t1 MTR_5g031000 43.662 71 38 1 1 71 1 69 9.15e-14 70.5
MS.gene58422.t1 MTR_7g075850 41.333 75 44 0 1 75 1 75 1.72e-13 70.1
MS.gene58422.t1 MTR_5g021270 38.710 124 71 4 1 120 1 123 2.70e-13 68.9
MS.gene58422.t1 MTR_7g075870 29.670 182 99 5 1 180 1 155 2.81e-13 68.6
MS.gene58422.t1 MTR_8g097090 32.057 209 106 10 1 188 1 194 2.95e-13 69.3
MS.gene58422.t1 MTR_5g021270 38.710 124 71 4 1 120 1 123 3.17e-13 68.9
MS.gene58422.t1 MTR_1g108510 29.762 168 104 6 2 167 9 164 3.22e-13 68.9
MS.gene58422.t1 MTR_1g054265 38.961 77 45 1 1 77 1 75 3.25e-13 67.4
MS.gene58422.t1 MTR_3g030770 32.558 129 82 4 2 128 9 134 5.16e-13 68.2
MS.gene58422.t1 MTR_4g102530 56.604 53 23 0 1 53 1 53 6.65e-13 67.4
MS.gene58422.t1 MTR_7g016600 31.343 201 109 8 1 184 1 189 6.88e-13 68.2
MS.gene58422.t1 MTR_8g033270 52.830 53 25 0 1 53 1 53 6.99e-13 68.2
MS.gene58422.t1 MTR_4g093030 54.717 53 24 0 1 53 1 53 8.90e-13 65.9
MS.gene58422.t1 MTR_4g109810 50.943 53 26 0 1 53 1 53 8.93e-13 67.8
MS.gene58422.t1 MTR_5g046870 52.830 53 25 0 1 53 1 53 9.56e-13 64.7
MS.gene58422.t1 MTR_8g043650 28.977 176 103 7 2 167 9 172 1.26e-12 67.4
MS.gene58422.t1 MTR_8g086290 51.923 52 25 0 2 53 12 63 1.36e-12 65.9
MS.gene58422.t1 MTR_1g041615 37.363 91 51 2 16 104 20 106 1.62e-12 64.7
MS.gene58422.t1 MTR_8g033220 50.943 53 26 0 1 53 1 53 2.55e-12 66.2
MS.gene58422.t1 MTR_8g033220 50.943 53 26 0 1 53 1 53 2.64e-12 66.2
MS.gene58422.t1 MTR_6g015975 50.943 53 26 0 1 53 1 53 2.79e-12 66.2
MS.gene58422.t1 MTR_3g084980 50.943 53 26 0 1 53 1 53 3.22e-12 66.2
MS.gene58422.t1 MTR_7g016630 28.977 176 113 4 1 169 1 171 3.23e-12 66.2
MS.gene58422.t1 MTR_8g051580 30.247 162 80 5 1 156 1 135 3.45e-12 64.7
MS.gene58422.t1 MTR_4g094638 53.704 54 25 0 1 54 1 54 3.49e-12 64.7
MS.gene58422.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 3.50e-12 64.7
MS.gene58422.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 4.35e-12 64.7
MS.gene58422.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 4.61e-12 64.7
MS.gene58422.t1 MTR_4g036050 41.333 75 43 1 1 75 1 74 4.82e-12 64.7
MS.gene58422.t1 MTR_1g108580 38.636 88 51 2 2 89 9 93 5.89e-12 65.1
MS.gene58422.t1 MTR_3g052870 33.884 121 73 5 2 119 9 125 7.34e-12 64.7
MS.gene58422.t1 MTR_6g464720 43.243 74 39 2 1 73 1 72 7.52e-12 65.1
MS.gene58422.t1 MTR_1g105920 33.884 121 73 5 2 119 9 125 1.23e-11 64.3
MS.gene58422.t1 MTR_2g105290 34.146 123 56 2 1 98 11 133 1.27e-11 63.2
MS.gene58422.t1 MTR_4g131030 40.260 77 44 1 1 77 1 75 1.38e-11 63.9
MS.gene58422.t1 MTR_3g052920 39.326 89 49 3 2 89 9 93 1.78e-11 63.9
MS.gene58422.t1 MTR_0121s0100 41.333 75 42 1 2 76 9 81 2.66e-11 61.2
MS.gene58422.t1 MTR_1g063160 37.662 77 46 1 1 77 1 75 2.78e-11 62.0
MS.gene58422.t1 MTR_5g045560 39.744 78 45 1 1 78 8 83 3.32e-11 63.2
MS.gene58422.t1 MTR_1g105910 38.202 89 50 3 2 89 9 93 3.32e-11 63.2
MS.gene58422.t1 MTR_8g079502 46.296 54 29 0 1 54 1 54 3.33e-11 62.0
MS.gene58422.t1 MTR_2g085250 38.043 92 54 2 2 93 9 97 3.55e-11 63.2
MS.gene58422.t1 MTR_8g046350 30.075 133 66 3 1 133 1 106 3.58e-11 61.6
MS.gene58422.t1 MTR_2g085280 38.043 92 54 2 2 93 9 97 3.59e-11 63.2
MS.gene58422.t1 MTR_1g075600 29.293 198 113 10 2 195 9 183 3.76e-11 62.8
MS.gene58422.t1 MTR_2g030740 38.202 89 50 3 2 89 9 93 6.50e-11 62.4
MS.gene58422.t1 MTR_1g077360 42.857 77 41 2 2 77 9 83 7.28e-11 62.0
MS.gene58422.t1 MTR_4g094632 48.148 54 28 0 1 54 1 54 7.32e-11 60.5
MS.gene58422.t1 MTR_1g047550 46.296 54 29 0 1 54 1 54 7.43e-11 60.8
MS.gene58422.t1 MTR_2g093190 40.000 75 43 1 2 76 9 81 8.72e-11 61.6
MS.gene58422.t1 MTR_5g055100 42.857 77 41 2 2 77 9 83 9.39e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58422.t1 AT1G18750 52.492 301 124 7 1 296 1 287 4.07e-96 291
MS.gene58422.t1 AT1G18750 52.492 301 124 7 1 296 1 287 7.20e-96 290
MS.gene58422.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.00e-89 273
MS.gene58422.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.00e-89 273
MS.gene58422.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.00e-89 273
MS.gene58422.t1 AT2G03060 51.930 285 113 5 1 276 1 270 3.23e-89 273
MS.gene58422.t1 AT1G18750 47.305 334 124 8 1 296 1 320 5.89e-89 273
MS.gene58422.t1 AT1G69540 43.322 307 144 8 1 292 1 292 1.74e-70 224
MS.gene58422.t1 AT1G69540 45.421 273 138 5 1 264 1 271 4.59e-70 224
MS.gene58422.t1 AT1G18750 45.882 255 119 7 47 296 1 241 8.98e-65 209
MS.gene58422.t1 AT2G03060 43.627 204 91 5 82 276 24 212 1.40e-42 150
MS.gene58422.t1 AT2G03060 43.627 204 91 5 82 276 24 212 1.40e-42 150
MS.gene58422.t1 AT2G03060 43.627 204 91 5 82 276 24 212 1.40e-42 150
MS.gene58422.t1 AT1G77980 36.735 196 108 6 1 181 1 195 3.55e-28 112
MS.gene58422.t1 AT1G18750 35.897 195 106 6 107 296 5 185 8.10e-28 110
MS.gene58422.t1 AT1G18750 35.979 189 102 7 113 296 23 197 1.31e-25 104
MS.gene58422.t1 AT1G22130 33.500 200 118 5 1 186 1 199 1.74e-25 105
MS.gene58422.t1 AT1G77950 34.031 191 101 6 1 171 1 186 7.39e-24 99.4
MS.gene58422.t1 AT1G77950 34.031 191 101 6 1 171 1 186 7.39e-24 99.4
MS.gene58422.t1 AT1G77950 34.031 191 101 6 1 171 1 186 7.39e-24 99.4
MS.gene58422.t1 AT1G77950 34.031 191 101 6 1 171 1 186 7.39e-24 99.4
MS.gene58422.t1 AT1G69540 32.584 178 110 4 96 264 2 178 2.21e-21 92.4
MS.gene58422.t1 AT2G26320 47.561 82 43 0 1 82 17 98 4.68e-19 82.0
MS.gene58422.t1 AT2G03710 36.747 166 70 6 1 159 1 138 1.88e-17 81.6
MS.gene58422.t1 AT2G03710 36.747 166 70 6 1 159 1 138 2.12e-17 81.3
MS.gene58422.t1 AT2G03710 36.747 166 70 6 1 159 1 138 7.22e-17 78.2
MS.gene58422.t1 AT4G24540 32.787 183 91 6 1 180 1 154 4.84e-16 76.6
MS.gene58422.t1 AT5G13790 38.384 99 57 2 1 97 1 97 6.51e-16 77.4
MS.gene58422.t1 AT5G23260 47.887 71 37 0 1 71 1 71 1.91e-15 75.5
MS.gene58422.t1 AT5G23260 47.887 71 37 0 1 71 1 71 2.15e-15 75.5
MS.gene58422.t1 AT5G23260 47.887 71 37 0 1 71 17 87 2.79e-15 75.5
MS.gene58422.t1 AT5G23260 47.887 71 37 0 1 71 17 87 2.87e-15 75.5
MS.gene58422.t1 AT3G57390 42.353 85 47 1 1 85 1 83 3.16e-15 74.7
MS.gene58422.t1 AT3G57390 42.353 85 47 1 1 85 1 83 3.94e-15 74.7
MS.gene58422.t1 AT5G13790 43.056 72 39 1 1 72 1 70 5.05e-15 74.7
MS.gene58422.t1 AT3G57230 44.444 72 38 1 1 72 1 70 6.67e-15 71.6
MS.gene58422.t1 AT4G37435 26.531 196 108 6 1 178 1 178 1.01e-14 72.4
MS.gene58422.t1 AT2G22540 32.927 164 84 6 1 164 1 138 1.90e-14 72.8
MS.gene58422.t1 AT2G22540 32.927 164 84 6 1 164 1 138 1.90e-14 72.8
MS.gene58422.t1 AT1G26310 43.038 79 44 1 1 79 1 78 1.95e-14 72.8
MS.gene58422.t1 AT2G14210 43.590 78 43 1 1 78 1 77 2.10e-14 72.4
MS.gene58422.t1 AT3G57230 44.444 72 38 1 1 72 1 70 2.13e-14 72.4
MS.gene58422.t1 AT3G57230 44.444 72 38 1 1 72 1 70 2.13e-14 72.4
MS.gene58422.t1 AT2G14210 43.590 78 43 1 1 78 1 77 2.15e-14 72.4
MS.gene58422.t1 AT4G37940 45.070 71 37 1 1 71 1 69 2.86e-14 70.9
MS.gene58422.t1 AT2G45650 30.000 190 114 6 1 180 1 181 3.15e-14 72.0
MS.gene58422.t1 AT4G37940 45.070 71 37 1 1 71 1 69 3.36e-14 71.2
MS.gene58422.t1 AT5G51870 45.333 75 39 1 1 75 1 73 4.13e-14 70.1
MS.gene58422.t1 AT4G37940 45.070 71 37 1 1 71 1 69 4.14e-14 71.2
MS.gene58422.t1 AT1G69120 43.038 79 44 1 1 79 1 78 5.15e-14 71.2
MS.gene58422.t1 AT4G09960 43.662 71 38 1 1 71 1 69 6.10e-14 70.9
MS.gene58422.t1 AT2G45660 46.479 71 36 1 1 71 1 69 6.48e-14 69.3
MS.gene58422.t1 AT1G69120 43.038 79 44 1 1 79 1 78 7.01e-14 71.2
MS.gene58422.t1 AT2G22540 52.830 53 25 0 1 53 1 53 7.60e-14 70.9
MS.gene58422.t1 AT5G51870 45.333 75 39 1 1 75 1 73 8.42e-14 70.1
MS.gene58422.t1 AT4G09960 43.662 71 38 1 1 71 1 69 8.89e-14 70.5
MS.gene58422.t1 AT4G09960 43.662 71 38 1 1 71 2 70 9.20e-14 70.5
MS.gene58422.t1 AT5G51870 45.333 75 39 1 1 75 24 96 1.06e-13 69.7
MS.gene58422.t1 AT4G09960 43.662 71 38 1 1 71 11 79 1.09e-13 70.5
MS.gene58422.t1 AT2G45660 46.479 71 36 1 1 71 1 69 1.10e-13 70.1
MS.gene58422.t1 AT1G71692 42.500 80 45 1 1 80 1 79 1.14e-13 69.7
MS.gene58422.t1 AT3G58780 52.830 53 25 0 1 53 16 68 1.30e-13 70.1
MS.gene58422.t1 AT5G51870 45.333 75 39 1 1 75 1 73 1.39e-13 69.7
MS.gene58422.t1 AT3G58780 52.830 53 25 0 1 53 16 68 1.39e-13 70.1
MS.gene58422.t1 AT2G42830 52.830 53 25 0 1 53 16 68 1.48e-13 70.1
MS.gene58422.t1 AT2G42830 52.830 53 25 0 1 53 16 68 1.52e-13 70.1
MS.gene58422.t1 AT5G51870 45.333 75 39 1 1 75 11 83 1.59e-13 69.7
MS.gene58422.t1 AT3G58780 52.830 53 25 0 1 53 22 74 1.60e-13 70.1
MS.gene58422.t1 AT4G09960 43.662 71 38 1 1 71 27 95 1.94e-13 70.1
MS.gene58422.t1 AT3G58780 52.830 53 25 0 1 53 16 68 2.25e-13 70.1
MS.gene58422.t1 AT4G18960 53.846 52 24 0 2 53 18 69 2.32e-13 69.7
MS.gene58422.t1 AT5G20240 36.782 87 55 0 1 87 1 87 2.42e-13 67.4
MS.gene58422.t1 AT2G22630 43.750 80 41 2 1 78 1 78 2.88e-13 68.9
MS.gene58422.t1 AT2G22630 43.750 80 41 2 1 78 1 78 2.88e-13 68.9
MS.gene58422.t1 AT3G58780 52.830 53 25 0 1 53 16 68 3.20e-13 69.7
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.98e-13 65.5
MS.gene58422.t1 AT3G61120 56.604 53 23 0 1 53 1 53 4.11e-13 68.9
MS.gene58422.t1 AT5G60910 45.070 71 38 1 1 71 1 70 4.16e-13 68.9
MS.gene58422.t1 AT5G20240 50.943 53 26 0 1 53 1 53 4.74e-13 67.8
MS.gene58422.t1 AT4G09960 52.830 53 25 0 1 53 96 148 5.26e-13 69.7
MS.gene58422.t1 AT5G10140 52.830 53 25 0 1 53 1 53 5.28e-13 67.0
MS.gene58422.t1 AT3G54340 31.731 104 71 0 1 104 1 104 5.35e-13 68.2
MS.gene58422.t1 AT5G10140 52.830 53 25 0 1 53 1 53 5.97e-13 67.4
MS.gene58422.t1 AT3G30260 40.845 71 41 1 1 71 1 70 6.07e-13 68.6
MS.gene58422.t1 AT5G10140 52.830 53 25 0 1 53 1 53 6.10e-13 67.0
MS.gene58422.t1 AT5G10140 52.830 53 25 0 1 53 1 53 6.68e-13 67.0
MS.gene58422.t1 AT4G11880 52.830 53 25 0 1 53 1 53 1.31e-12 66.2
MS.gene58422.t1 AT4G11880 52.830 53 25 0 1 53 1 53 1.31e-12 66.2
MS.gene58422.t1 AT4G11880 52.830 53 25 0 1 53 1 53 2.55e-12 66.2
MS.gene58422.t1 AT4G11880 52.830 53 25 0 1 53 1 53 2.55e-12 66.2
MS.gene58422.t1 AT1G24260 50.943 53 26 0 1 53 1 53 2.62e-12 66.2
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.12e-12 64.3
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.12e-12 64.3
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.12e-12 64.3
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.12e-12 64.3
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 3.12e-12 64.3
MS.gene58422.t1 AT1G24260 50.943 53 26 0 1 53 1 53 3.15e-12 66.2
MS.gene58422.t1 AT5G51860 28.723 188 98 6 1 168 1 172 3.16e-12 65.5
MS.gene58422.t1 AT1G24260 50.943 53 26 0 1 53 1 53 3.19e-12 66.2
MS.gene58422.t1 AT5G62165 43.662 71 38 1 1 71 1 69 3.33e-12 64.3
MS.gene58422.t1 AT5G62165 43.662 71 38 1 1 71 1 69 3.33e-12 64.3
MS.gene58422.t1 AT5G62165 43.662 71 38 1 1 71 1 69 3.33e-12 64.3
MS.gene58422.t1 AT4G22950 26.519 181 106 3 1 181 1 154 3.74e-12 65.5
MS.gene58422.t1 AT4G22950 26.519 181 106 3 1 181 1 154 3.74e-12 65.5
MS.gene58422.t1 AT5G15800 50.943 53 26 0 1 53 1 53 3.89e-12 66.2
MS.gene58422.t1 AT5G62165 28.729 181 103 4 1 181 1 155 3.91e-12 65.5
MS.gene58422.t1 AT5G62165 28.729 181 103 4 1 181 1 155 3.91e-12 65.5
MS.gene58422.t1 AT5G62165 28.729 181 103 4 1 181 1 155 3.91e-12 65.5
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 4.27e-12 65.1
MS.gene58422.t1 AT5G62165 40.789 76 43 1 1 76 1 74 4.27e-12 65.1
MS.gene58422.t1 AT5G15800 50.943 53 26 0 1 53 1 53 4.67e-12 65.9
MS.gene58422.t1 AT3G02310 50.943 53 26 0 1 53 1 53 5.33e-12 64.7
MS.gene58422.t1 AT5G65050 34.211 152 84 5 1 147 1 141 6.61e-12 64.3
MS.gene58422.t1 AT5G51860 42.466 73 40 1 1 73 1 71 6.67e-12 64.7
MS.gene58422.t1 AT3G02310 50.943 53 26 0 1 53 1 53 7.44e-12 65.1
MS.gene58422.t1 AT5G65050 43.662 71 39 1 1 71 1 70 8.24e-12 63.9
MS.gene58422.t1 AT4G22950 50.943 53 26 0 1 53 1 53 9.75e-12 63.5
MS.gene58422.t1 AT2G34440 46.296 54 29 0 1 54 1 54 1.68e-11 62.8
MS.gene58422.t1 AT5G65050 43.662 71 39 1 1 71 1 70 1.71e-11 63.2
MS.gene58422.t1 AT5G65050 43.662 71 39 1 1 71 1 70 1.81e-11 62.8
MS.gene58422.t1 AT3G18650 26.667 225 152 5 14 231 29 247 2.29e-11 65.1
MS.gene58422.t1 AT5G65070 47.170 53 28 0 1 53 1 53 2.58e-11 61.6
MS.gene58422.t1 AT3G66656 46.296 54 29 0 1 54 1 54 4.25e-11 61.6
MS.gene58422.t1 AT1G01530 49.057 53 27 0 1 53 6 58 4.35e-11 62.8
MS.gene58422.t1 AT5G65070 47.170 53 28 0 1 53 1 53 4.79e-11 62.0
MS.gene58422.t1 AT4G36590 43.662 71 38 1 2 72 7 75 5.73e-11 62.8
MS.gene58422.t1 AT5G65070 47.170 53 28 0 1 53 1 53 7.93e-11 61.6
MS.gene58422.t1 AT5G65070 47.170 53 28 0 1 53 1 53 8.42e-11 62.0
MS.gene58422.t1 AT5G65070 47.170 53 28 0 1 53 1 53 8.63e-11 62.0

Find 87 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAATTGAAGATAAAGAAAT+TGG 0.209657 8.3:-15835981 MS.gene58422:CDS
GTTCTAATTGAGCAGGAAAA+TGG 0.237423 8.3:-15835314 MS.gene58422:intron
CCAAGCTATTCTGGTTATAC+TGG 0.263985 8.3:-15833594 MS.gene58422:CDS
TGCACATTTAAGTAAGGATT+TGG 0.267580 8.3:+15833486 None:intergenic
CCAAAATCACTATTTGAAAA+TGG 0.281681 8.3:-15833345 MS.gene58422:CDS
TTGAAAATGGACACCAGTTC+TGG 0.282537 8.3:-15833332 MS.gene58422:CDS
AACCAAAGTTTATGTCATTC+AGG 0.285801 8.3:-15833886 MS.gene58422:intron
TTGTAGACCAGACATCATTA+AGG 0.306825 8.3:+15833970 None:intergenic
ATTTCACTCCCAAGCTATTC+TGG 0.310454 8.3:-15833603 MS.gene58422:CDS
GTCCACAAGAAAGGGCTAAA+AGG 0.330693 8.3:-15835405 MS.gene58422:intron
GGTACCTTGAATGAGTTAAA+TGG 0.339481 8.3:-15833513 MS.gene58422:CDS
CGCATTCAAGTGACATTAGT+TGG 0.358372 8.3:+15834107 None:intergenic
TGTAAAGATACAAGATTTCC+TGG 0.370430 8.3:-15835127 MS.gene58422:CDS
CCCTTTCTTGTGGACTAAGT+TGG 0.379881 8.3:+15835413 None:intergenic
ACATGTCACTACTTTGGGTC+AGG 0.387955 8.3:-15833541 MS.gene58422:CDS
CCTTTCTTGTGGACTAAGTT+GGG 0.389274 8.3:+15835414 None:intergenic
AAGGGTAAAGTCATCCCTTC+TGG 0.392867 8.3:+15833989 None:intergenic
AGGAAAGCCTACGTTATTAC+AGG 0.394098 8.3:-15835840 MS.gene58422:CDS
CACTGTTGCACATTTAAGTA+AGG 0.404816 8.3:+15833480 None:intergenic
CTCTCCACATGTCACTACTT+TGG 0.406741 8.3:-15833547 MS.gene58422:CDS
TCTCAGTTATTGGAGTAATC+TGG 0.414099 8.3:-15834355 MS.gene58422:intron
TCTCCACATGTCACTACTTT+GGG 0.420528 8.3:-15833546 MS.gene58422:CDS
ACTTTACCCTTAATGATGTC+TGG 0.424560 8.3:-15833977 MS.gene58422:CDS
CCCAACTTAGTCCACAAGAA+AGG 0.426307 8.3:-15835414 MS.gene58422:CDS
ATCATGATCCAATTTCTTGA+AGG 0.431577 8.3:+15835149 None:intergenic
CTTGGAACATCTTAGACAAA+TGG 0.436675 8.3:-15834319 MS.gene58422:CDS
TTATTTGATTTGATATTGAT+TGG 0.438851 8.3:+15833402 None:intergenic
GTTCACTAGGTAACATCAAC+TGG 0.439721 8.3:+15833906 None:intergenic
CACCTGAATGACATAAACTT+TGG 0.443645 8.3:+15833884 None:intergenic
CAATCGATTGTCTATACAAA+AGG 0.445203 8.3:-15834274 MS.gene58422:intron
TGTAGACCAGACATCATTAA+GGG 0.446718 8.3:+15833971 None:intergenic
TTCACTTCCTCGAGATCTTG+TGG 0.468977 8.3:+15833435 None:intergenic
GATCATAATTGTTATTAACT+TGG 0.470376 8.3:+15833370 None:intergenic
AAGAATCTCAACCTTTGTCT+TGG 0.471192 8.3:-15833949 MS.gene58422:CDS
TACAAGATTTCCTGGGTTCA+AGG 0.476006 8.3:-15835119 MS.gene58422:intron
ATTTCCATTTAACTCATTCA+AGG 0.477610 8.3:+15833509 None:intergenic
TTATACTGGGAACTGCAAAC+TGG 0.478109 8.3:-15833580 MS.gene58422:CDS
ATTGCTATGTATAACGAGAA+TGG 0.479596 8.3:-15833285 MS.gene58422:CDS
TGGGAACTGCAAACTGGAGG+TGG 0.480619 8.3:-15833574 MS.gene58422:CDS
AGATTCTCACTTGATGACTT+AGG 0.481817 8.3:+15834890 None:intergenic
CAGTATAACCAGAATAGCTT+GGG 0.488834 8.3:+15833595 None:intergenic
TTGATTTGATATTGATTGGT+TGG 0.490496 8.3:+15833406 None:intergenic
TCCTCGAGATCTTGTGGAGG+AGG 0.496372 8.3:+15833441 None:intergenic
AAAACTACATGCTTACCCTA+TGG 0.497229 8.3:+15833259 None:intergenic
ACTACTTTGGGTCAGGGTGG+TGG 0.504351 8.3:-15833534 MS.gene58422:CDS
TCTGGAGAAGATCAATAACT+TGG 0.508786 8.3:-15834337 MS.gene58422:CDS
CATTTGTAGAATTCCAGAAC+TGG 0.509091 8.3:+15833319 None:intergenic
CCAGTATAACCAGAATAGCT+TGG 0.510057 8.3:+15833594 None:intergenic
GTCACTTGAATGCGTTAACC+AGG 0.524046 8.3:-15834098 MS.gene58422:intron
ACTTATTCGAAGCGGAAAAG+TGG 0.527998 8.3:-15835935 MS.gene58422:CDS
CCAACTTAGTCCACAAGAAA+GGG 0.532263 8.3:-15835413 MS.gene58422:CDS
CAAGCTATTCTGGTTATACT+GGG 0.535943 8.3:-15833593 MS.gene58422:CDS
GACAAATGGAAGACTCACTC+AGG 0.540615 8.3:-15834305 MS.gene58422:CDS
CCTTTGTCTTGGCTTCTAAG+TGG 0.542337 8.3:-15833938 MS.gene58422:CDS
AAGTGAGAATCTTACAAGCT+CGG 0.547873 8.3:-15834879 MS.gene58422:CDS
ATAACGAGAATGGTTTCCAT+AGG 0.556985 8.3:-15833275 MS.gene58422:CDS
ATTGATTCATAGTTACCAGA+AGG 0.565773 8.3:-15834004 MS.gene58422:intron
CATGTCACTACTTTGGGTCA+GGG 0.574709 8.3:-15833540 MS.gene58422:CDS
CTGTTGGAGAAAACATGAGA+AGG 0.576749 8.3:+15835861 None:intergenic
CTTATTCGAAGCGGAAAAGT+GGG 0.585417 8.3:-15835934 MS.gene58422:CDS
CAATCACTTACCTTGAACCC+AGG 0.589742 8.3:+15835109 None:intergenic
ATTACGTTACATAGTGACAA+TGG 0.595952 8.3:-15833654 MS.gene58422:intron
CGTTCTCCCTGTAATAACGT+AGG 0.597125 8.3:+15835833 None:intergenic
GTAAAGATACAAGATTTCCT+GGG 0.597719 8.3:-15835126 MS.gene58422:CDS
TAACGAGAATGGTTTCCATA+GGG 0.598896 8.3:-15833274 MS.gene58422:CDS
CCCTCCTCCACAAGATCTCG+AGG 0.601305 8.3:-15833442 MS.gene58422:CDS
CCTCGAGATCTTGTGGAGGA+GGG 0.602901 8.3:+15833442 None:intergenic
CAATATCAAATCAAATAACG+TGG 0.608707 8.3:-15833397 MS.gene58422:CDS
ATAGCTTGGGAGTGAAATGT+TGG 0.609782 8.3:+15833608 None:intergenic
GTCACTACTTTGGGTCAGGG+TGG 0.612222 8.3:-15833537 MS.gene58422:CDS
GACATAAACTTTGGTTCACT+AGG 0.613858 8.3:+15833893 None:intergenic
GGGAACTGCAAACTGGAGGT+GGG 0.623032 8.3:-15833573 MS.gene58422:CDS
AATAACGTAGGCTTTCCTGT+TGG 0.623309 8.3:+15835845 None:intergenic
AATTCTACAAATGACTCTTG+TGG 0.631946 8.3:-15833309 MS.gene58422:CDS
TTGATTCATAGTTACCAGAA+GGG 0.636080 8.3:-15834003 MS.gene58422:intron
ACTTCCTCGAGATCTTGTGG+AGG 0.636550 8.3:+15833438 None:intergenic
CAGAAATACAACAGAGACTG+AGG 0.637183 8.3:-15834852 MS.gene58422:intron
ATATTGCAGCAGTCAAACAG+TGG 0.648674 8.3:-15835020 MS.gene58422:intron
TACTGGGAACTGCAAACTGG+AGG 0.648889 8.3:-15833577 MS.gene58422:CDS
GCTTCGAATAAGTCACATGT+CGG 0.667125 8.3:+15835945 None:intergenic
ACAAATGGAAGACTCACTCA+GGG 0.670210 8.3:-15834304 MS.gene58422:CDS
TGACCCAAAGTAGTGACATG+TGG 0.672596 8.3:+15833543 None:intergenic
GGAAAGCCTACGTTATTACA+GGG 0.679926 8.3:-15835839 MS.gene58422:CDS
GACATGTGACTTATTCGAAG+CGG 0.683369 8.3:-15835943 MS.gene58422:CDS
CCACTTAGAAGCCAAGACAA+AGG 0.694579 8.3:+15833938 None:intergenic
TTGCAGCAGTCAAACAGTGG+AGG 0.697141 8.3:-15835017 MS.gene58422:intron
CGTTATTACAGGGAGAACGA+AGG 0.718082 8.3:-15835829 MS.gene58422:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATAGATAGAAAAAA+GGG + chr8.3:15835201-15835220 None:intergenic 10.0%
!! TATAATAATAGATAGAAAAA+AGG + chr8.3:15835202-15835221 None:intergenic 10.0%
!!! GTTCAAATATTTTAAAAAAA+TGG - chr8.3:15833628-15833647 MS.gene58422:CDS 10.0%
!!! TAGTATATCTATTTTTTTTA+AGG + chr8.3:15833691-15833710 None:intergenic 10.0%
!! TAAATTGAAGATAAAGAAAT+TGG - chr8.3:15833274-15833293 MS.gene58422:CDS 15.0%
!! TTATTCACAAATTATCAAAT+GGG - chr8.3:15834267-15834286 MS.gene58422:intron 15.0%
!! TTTATTCACAAATTATCAAA+TGG - chr8.3:15834266-15834285 MS.gene58422:intron 15.0%
!!! TATTGATATTTTGATTTCAT+CGG - chr8.3:15835105-15835124 MS.gene58422:intron 15.0%
!!! TTATTTGATTTGATATTGAT+TGG + chr8.3:15835856-15835875 None:intergenic 15.0%
!! AAAATACACTATTATTTACC+TGG + chr8.3:15835178-15835197 None:intergenic 20.0%
!! AAAGTAATATATGAATTACG+AGG + chr8.3:15833996-15834015 None:intergenic 20.0%
!! AATCTTCAAAATCATTTACA+AGG + chr8.3:15834196-15834215 None:intergenic 20.0%
!! ATATGTAATATCTCAGTTAT+TGG - chr8.3:15834890-15834909 MS.gene58422:CDS 20.0%
!! CGAAAAATTTAAATTGAACA+AGG + chr8.3:15834597-15834616 None:intergenic 20.0%
!! GTCAAATACTATCATAATTT+GGG - chr8.3:15834842-15834861 MS.gene58422:intron 20.0%
!! TCTATACAAAAGGTAATATT+TGG - chr8.3:15834991-15835010 MS.gene58422:intron 20.0%
!! TGATAGTATTTGACATAATT+TGG + chr8.3:15834836-15834855 None:intergenic 20.0%
!! TGCAATATGAAAATTAAAAC+AGG + chr8.3:15834223-15834242 None:intergenic 20.0%
!! TGTCAAATACTATCATAATT+TGG - chr8.3:15834841-15834860 MS.gene58422:intron 20.0%
!!! ACTTATCATTTTAATCTCAA+AGG - chr8.3:15833600-15833619 MS.gene58422:CDS 20.0%
!!! ATTTTATTTTTCCAGCAATA+TGG - chr8.3:15833797-15833816 MS.gene58422:intron 20.0%
!!! GATCATAATTGTTATTAACT+TGG + chr8.3:15835888-15835907 None:intergenic 20.0%
!!! TTTGGTCTTGTTTAAAATTT+AGG - chr8.3:15834071-15834090 MS.gene58422:intron 20.0%
! ATTTCCATTTAACTCATTCA+AGG + chr8.3:15835749-15835768 None:intergenic 25.0%
! CAATATCAAATCAAATAACG+TGG - chr8.3:15835858-15835877 MS.gene58422:CDS 25.0%
! CCAAAATCACTATTTGAAAA+TGG - chr8.3:15835910-15835929 MS.gene58422:CDS 25.0%
! CGAAGGTAATGTAATAATAA+CGG - chr8.3:15833443-15833462 MS.gene58422:CDS 25.0%
! CTTGTGATAAAATGAGAAAA+TGG - chr8.3:15833519-15833538 MS.gene58422:CDS 25.0%
! GAAAAAAACCTTCAAGAAAT+TGG - chr8.3:15834098-15834117 MS.gene58422:intron 25.0%
! TACACACTCATAATTAATAC+AGG + chr8.3:15834562-15834581 None:intergenic 25.0%
! TCAGTCATTGCTTAAAAATT+TGG - chr8.3:15834053-15834072 MS.gene58422:intron 25.0%
!! AAAGAGCTTTATAACAAAAG+TGG - chr8.3:15835420-15835439 MS.gene58422:CDS 25.0%
!! AGTTTGTACCAAAAAAGTAT+AGG + chr8.3:15834795-15834814 None:intergenic 25.0%
!! CCATTTTCAAATAGTGATTT+TGG + chr8.3:15835913-15835932 None:intergenic 25.0%
!! TAATCACAACGATTTTCTAA+GGG + chr8.3:15835066-15835085 None:intergenic 25.0%
!! TTAATCACAACGATTTTCTA+AGG + chr8.3:15835067-15835086 None:intergenic 25.0%
!! TTGATTTGATATTGATTGGT+TGG + chr8.3:15835852-15835871 None:intergenic 25.0%
!! TTTCTCATTTTATCACAAGA+AGG + chr8.3:15833518-15833537 None:intergenic 25.0%
!!! ACTTTTACACACTTTAAAGA+TGG + chr8.3:15833728-15833747 None:intergenic 25.0%
!!! ATCATTTTAATCTCAAAGGA+CGG - chr8.3:15833604-15833623 MS.gene58422:CDS 25.0%
!!! CGACAAACACATTTTTTTTT+GGG + chr8.3:15834769-15834788 None:intergenic 25.0%
!!! TCGACAAACACATTTTTTTT+TGG + chr8.3:15834770-15834789 None:intergenic 25.0%
AACCAAAGTTTATGTCATTC+AGG - chr8.3:15835369-15835388 MS.gene58422:intron 30.0%
AAGCACATACAAAATTGGTA+AGG + chr8.3:15834524-15834543 None:intergenic 30.0%
AATTCTACAAATGACTCTTG+TGG - chr8.3:15835946-15835965 MS.gene58422:CDS 30.0%
AGAATCTTAAGCTGGTAATT+AGG - chr8.3:15834304-15834323 MS.gene58422:CDS 30.0%
ATACAAAATTGGTAAGGTAG+AGG + chr8.3:15834518-15834537 None:intergenic 30.0%
ATCATGATCCAATTTCTTGA+AGG + chr8.3:15834109-15834128 None:intergenic 30.0%
ATTACGTTACATAGTGACAA+TGG - chr8.3:15835601-15835620 MS.gene58422:intron 30.0%
ATTGCTATGTATAACGAGAA+TGG - chr8.3:15835970-15835989 MS.gene58422:CDS 30.0%
CAATCGATTGTCTATACAAA+AGG - chr8.3:15834981-15835000 MS.gene58422:intron 30.0%
CAATTGAACTCATAACACTT+TGG + chr8.3:15834333-15834352 None:intergenic 30.0%
GTAAAGATACAAGATTTCCT+GGG - chr8.3:15834129-15834148 MS.gene58422:intron 30.0%
TGCACATTTAAGTAAGGATT+TGG + chr8.3:15835772-15835791 None:intergenic 30.0%
TGTAAAGATACAAGATTTCC+TGG - chr8.3:15834128-15834147 MS.gene58422:intron 30.0%
TTAAAGTGTGTAAAAGTCGA+AGG - chr8.3:15833731-15833750 MS.gene58422:intron 30.0%
TTGATTCATAGTTACCAGAA+GGG - chr8.3:15835252-15835271 MS.gene58422:intron 30.0%
TTGCAAAGCACATACAAAAT+TGG + chr8.3:15834529-15834548 None:intergenic 30.0%
! AATCACAACGATTTTCTAAG+GGG + chr8.3:15835065-15835084 None:intergenic 30.0%
! AGGTAATATTTGGTTTCATG+CGG - chr8.3:15835001-15835020 MS.gene58422:intron 30.0%
! ATTGATTCATAGTTACCAGA+AGG - chr8.3:15835251-15835270 MS.gene58422:intron 30.0%
! TTTTCTTGTTCTAATTGAGC+AGG - chr8.3:15833934-15833953 MS.gene58422:CDS 30.0%
!! ATTGCTTTAGAATCTTAAGC+TGG - chr8.3:15834296-15834315 MS.gene58422:CDS 30.0%
!! TTATTTTTCCAGCAATATGG+AGG - chr8.3:15833800-15833819 MS.gene58422:intron 30.0%
!!! CAAACACATTTTTTTTTGGG+TGG + chr8.3:15834766-15834785 None:intergenic 30.0%
!!! TGTCGAAACCTATACTTTTT+TGG - chr8.3:15834784-15834803 MS.gene58422:intron 30.0%
!!! TTTTTTGGGTGGTGTTTATT+TGG + chr8.3:15834755-15834774 None:intergenic 30.0%
AAGAATCTCAACCTTTGTCT+TGG - chr8.3:15835306-15835325 MS.gene58422:intron 35.0%
AAGTGAGAATCTTACAAGCT+CGG - chr8.3:15834376-15834395 MS.gene58422:intron 35.0%
ACTTTACCCTTAATGATGTC+TGG - chr8.3:15835278-15835297 MS.gene58422:intron 35.0%
ATAACGAGAATGGTTTCCAT+AGG - chr8.3:15835980-15835999 MS.gene58422:CDS 35.0%
CACCTGAATGACATAAACTT+TGG + chr8.3:15835374-15835393 None:intergenic 35.0%
CACTGTTGCACATTTAAGTA+AGG + chr8.3:15835778-15835797 None:intergenic 35.0%
CAGTATAACCAGAATAGCTT+GGG + chr8.3:15835663-15835682 None:intergenic 35.0%
CATTTGTAGAATTCCAGAAC+TGG + chr8.3:15835939-15835958 None:intergenic 35.0%
CTTGGAACATCTTAGACAAA+TGG - chr8.3:15834936-15834955 MS.gene58422:intron 35.0%
GGTACCTTGAATGAGTTAAA+TGG - chr8.3:15835742-15835761 MS.gene58422:intron 35.0%
GTTCTAATTGAGCAGGAAAA+TGG - chr8.3:15833941-15833960 MS.gene58422:CDS 35.0%
TAACGAGAATGGTTTCCATA+GGG - chr8.3:15835981-15836000 MS.gene58422:CDS 35.0%
TCTCAGTTATTGGAGTAATC+TGG - chr8.3:15834900-15834919 MS.gene58422:intron 35.0%
TCTGGAGAAGATCAATAACT+TGG - chr8.3:15834918-15834937 MS.gene58422:intron 35.0%
TGTAGACCAGACATCATTAA+GGG + chr8.3:15835287-15835306 None:intergenic 35.0%
TTGTAGACCAGACATCATTA+AGG + chr8.3:15835288-15835307 None:intergenic 35.0%
! AGATTCTCACTTGATGACTT+AGG + chr8.3:15834368-15834387 None:intergenic 35.0%
! CAAGCTATTCTGGTTATACT+GGG - chr8.3:15835662-15835681 MS.gene58422:intron 35.0%
! GACATAAACTTTGGTTCACT+AGG + chr8.3:15835365-15835384 None:intergenic 35.0%
!! GTTTATTTGGTGTGAGCTAA+AGG + chr8.3:15834742-15834761 None:intergenic 35.0%
!!! GATATTTTGATTTCATCGGC+AGG - chr8.3:15835109-15835128 MS.gene58422:intron 35.0%
AATAACGTAGGCTTTCCTGT+TGG + chr8.3:15833413-15833432 None:intergenic 40.0%
AATGATCTCCTCCATATTGC+TGG + chr8.3:15833811-15833830 None:intergenic 40.0%
ACAAATGGAAGACTCACTCA+GGG - chr8.3:15834951-15834970 MS.gene58422:intron 40.0%
ACTTATTCGAAGCGGAAAAG+TGG - chr8.3:15833320-15833339 MS.gene58422:CDS 40.0%
AGGAAAGCCTACGTTATTAC+AGG - chr8.3:15833415-15833434 MS.gene58422:CDS 40.0%
ATAGCTTGGGAGTGAAATGT+TGG + chr8.3:15835650-15835669 None:intergenic 40.0%
ATATTGCAGCAGTCAAACAG+TGG - chr8.3:15834235-15834254 MS.gene58422:intron 40.0%
ATTTCACTCCCAAGCTATTC+TGG - chr8.3:15835652-15835671 MS.gene58422:intron 40.0%
CAGAAATACAACAGAGACTG+AGG - chr8.3:15834403-15834422 MS.gene58422:intron 40.0%
CCAACTTAGTCCACAAGAAA+GGG - chr8.3:15833842-15833861 MS.gene58422:intron 40.0%
CCAGTATAACCAGAATAGCT+TGG + chr8.3:15835664-15835683 None:intergenic 40.0%
CGCATTCAAGTGACATTAGT+TGG + chr8.3:15835151-15835170 None:intergenic 40.0%
CTGTTGGAGAAAACATGAGA+AGG + chr8.3:15833397-15833416 None:intergenic 40.0%
CTTATTCGAAGCGGAAAAGT+GGG - chr8.3:15833321-15833340 MS.gene58422:CDS 40.0%
GACATGTGACTTATTCGAAG+CGG - chr8.3:15833312-15833331 MS.gene58422:CDS 40.0%
GCTTCGAATAAGTCACATGT+CGG + chr8.3:15833313-15833332 None:intergenic 40.0%
GGAAAGCCTACGTTATTACA+GGG - chr8.3:15833416-15833435 MS.gene58422:CDS 40.0%
GTTCACTAGGTAACATCAAC+TGG + chr8.3:15835352-15835371 None:intergenic 40.0%
TACAAGATTTCCTGGGTTCA+AGG - chr8.3:15834136-15834155 MS.gene58422:intron 40.0%
TCTCCACATGTCACTACTTT+GGG - chr8.3:15835709-15835728 MS.gene58422:intron 40.0%
TTATACTGGGAACTGCAAAC+TGG - chr8.3:15835675-15835694 MS.gene58422:intron 40.0%
TTGAAAATGGACACCAGTTC+TGG - chr8.3:15835923-15835942 MS.gene58422:CDS 40.0%
! AACCTTTTAGCCCTTTCTTG+TGG + chr8.3:15833855-15833874 None:intergenic 40.0%
! CCAAGCTATTCTGGTTATAC+TGG - chr8.3:15835661-15835680 MS.gene58422:intron 40.0%
! CCTTTCTTGTGGACTAAGTT+GGG + chr8.3:15833844-15833863 None:intergenic 40.0%
! CTTCTCATGTTTTCTCCAAC+AGG - chr8.3:15833395-15833414 MS.gene58422:CDS 40.0%
! TGAGTTCAATTGCCATTTGC+AGG - chr8.3:15834341-15834360 MS.gene58422:CDS 40.0%
!! ACAACGATTTTCTAAGGGGA+CGG + chr8.3:15835061-15835080 None:intergenic 40.0%
AAGGGTAAAGTCATCCCTTC+TGG + chr8.3:15835269-15835288 None:intergenic 45.0%
ACATGTCACTACTTTGGGTC+AGG - chr8.3:15835714-15835733 MS.gene58422:intron 45.0%
CAATCACTTACCTTGAACCC+AGG + chr8.3:15834149-15834168 None:intergenic 45.0%
CATGTCACTACTTTGGGTCA+GGG - chr8.3:15835715-15835734 MS.gene58422:intron 45.0%
CCACTTAGAAGCCAAGACAA+AGG + chr8.3:15835320-15835339 None:intergenic 45.0%
CCCAACTTAGTCCACAAGAA+AGG - chr8.3:15833841-15833860 MS.gene58422:intron 45.0%
CCTTTGTCTTGGCTTCTAAG+TGG - chr8.3:15835317-15835336 MS.gene58422:intron 45.0%
CGTTATTACAGGGAGAACGA+AGG - chr8.3:15833426-15833445 MS.gene58422:CDS 45.0%
CGTTCTCCCTGTAATAACGT+AGG + chr8.3:15833425-15833444 None:intergenic 45.0%
CTCTCCACATGTCACTACTT+TGG - chr8.3:15835708-15835727 MS.gene58422:intron 45.0%
GACAAATGGAAGACTCACTC+AGG - chr8.3:15834950-15834969 MS.gene58422:intron 45.0%
GATGACTTAGGTCCTGCAAA+TGG + chr8.3:15834356-15834375 None:intergenic 45.0%
GTCACTTGAATGCGTTAACC+AGG - chr8.3:15835157-15835176 MS.gene58422:CDS 45.0%
GTCCACAAGAAAGGGCTAAA+AGG - chr8.3:15833850-15833869 MS.gene58422:intron 45.0%
TGACCCAAAGTAGTGACATG+TGG + chr8.3:15835715-15835734 None:intergenic 45.0%
TTCACTTCCTCGAGATCTTG+TGG + chr8.3:15835823-15835842 None:intergenic 45.0%
! CCCTTTCTTGTGGACTAAGT+TGG + chr8.3:15833845-15833864 None:intergenic 45.0%
! TTGGTGTGAGCTAAAGGCAT+GGG + chr8.3:15834736-15834755 None:intergenic 45.0%
! TTTGGTGTGAGCTAAAGGCA+TGG + chr8.3:15834737-15834756 None:intergenic 45.0%
!! CAACGATTTTCTAAGGGGAC+GGG + chr8.3:15835060-15835079 None:intergenic 45.0%
!!! AGGTGCAACTTTGGAGCTTT+TGG - chr8.3:15834423-15834442 MS.gene58422:intron 45.0%
!! AAATAAAAATAAATATTTAG+TGG + chr8.3:15833890-15833909 None:intergenic 5.0%
!! AATAAAAATAAATATTTAGT+GGG + chr8.3:15833889-15833908 None:intergenic 5.0%
ACTTCCTCGAGATCTTGTGG+AGG + chr8.3:15835820-15835839 None:intergenic 50.0%
CAGAGACTGAGGTGCAACTT+TGG - chr8.3:15834414-15834433 MS.gene58422:intron 50.0%
TACTGGGAACTGCAAACTGG+AGG - chr8.3:15835678-15835697 MS.gene58422:intron 50.0%
TTGCAGCAGTCAAACAGTGG+AGG - chr8.3:15834238-15834257 MS.gene58422:intron 50.0%
! TGGTGTGAGCTAAAGGCATG+GGG + chr8.3:15834735-15834754 None:intergenic 50.0%
ACTACTTTGGGTCAGGGTGG+TGG - chr8.3:15835721-15835740 MS.gene58422:intron 55.0%
CCTCGAGATCTTGTGGAGGA+GGG + chr8.3:15835816-15835835 None:intergenic 55.0%
GGGAACTGCAAACTGGAGGT+GGG - chr8.3:15835682-15835701 MS.gene58422:intron 55.0%
GTCACTACTTTGGGTCAGGG+TGG - chr8.3:15835718-15835737 MS.gene58422:intron 55.0%
TCCTCGAGATCTTGTGGAGG+AGG + chr8.3:15835817-15835836 None:intergenic 55.0%
TGGGAACTGCAAACTGGAGG+TGG - chr8.3:15835681-15835700 MS.gene58422:intron 55.0%
! TTCTAAGGGGACGGGAACGA+TGG + chr8.3:15835052-15835071 None:intergenic 55.0%
CCCTCCTCCACAAGATCTCG+AGG - chr8.3:15835813-15835832 MS.gene58422:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 15833263 15836014 15833263 ID=MS.gene58422
chr8.3 mRNA 15833263 15836014 15833263 ID=MS.gene58422.t1;Parent=MS.gene58422
chr8.3 exon 15835830 15836014 15835830 ID=MS.gene58422.t1.exon1;Parent=MS.gene58422.t1
chr8.3 CDS 15835830 15836014 15835830 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15835406 15835465 15835406 ID=MS.gene58422.t1.exon2;Parent=MS.gene58422.t1
chr8.3 CDS 15835406 15835465 15835406 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15835303 15835321 15835303 ID=MS.gene58422.t1.exon3;Parent=MS.gene58422.t1
chr8.3 CDS 15835303 15835321 15835303 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15835120 15835184 15835120 ID=MS.gene58422.t1.exon4;Parent=MS.gene58422.t1
chr8.3 CDS 15835120 15835184 15835120 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15835018 15835033 15835018 ID=MS.gene58422.t1.exon5;Parent=MS.gene58422.t1
chr8.3 CDS 15835018 15835033 15835018 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15834853 15834914 15834853 ID=MS.gene58422.t1.exon6;Parent=MS.gene58422.t1
chr8.3 CDS 15834853 15834914 15834853 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15834275 15834371 15834275 ID=MS.gene58422.t1.exon7;Parent=MS.gene58422.t1
chr8.3 CDS 15834275 15834371 15834275 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15834099 15834146 15834099 ID=MS.gene58422.t1.exon8;Parent=MS.gene58422.t1
chr8.3 CDS 15834099 15834146 15834099 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15833887 15834014 15833887 ID=MS.gene58422.t1.exon9;Parent=MS.gene58422.t1
chr8.3 CDS 15833887 15834014 15833887 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
chr8.3 exon 15833263 15833662 15833263 ID=MS.gene58422.t1.exon10;Parent=MS.gene58422.t1
chr8.3 CDS 15833263 15833662 15833263 ID=cds.MS.gene58422.t1;Parent=MS.gene58422.t1
Gene Sequence

>MS.gene58422

ATGGGGAGAGTTAAATTGAAGATAAAGAAATTGGAGAGCACAAGCAACCGACATGTGACTTATTCGAAGCGGAAAAGTGGGATCTTGAAGAAAGCAAAAGAGTTATCAATTCTATGTGATATAGATATTCTCCTTCTCATGTTTTCTCCAACAGGAAAGCCTACGTTATTACAGGGAGAACGAAGGTAATGTAATAATAACGGCGAAGCATTAATATGTGTCATATGTTTCTAGTACGACGAACAATTGTTTCCTTCTTGTGATAAAATGAGAAAATGGTACAATAATTGTTGAATGATTAACATGCGTCGTCGATTGATCAATATGTGAATCACACACTTATCATTTTAATCTCAAAGGACGGTGTTCAAATATTTTAAAAAAATGGTTGTAACATGAACTTTAGTGTCGAATAAGAGAGTTTTCCTTAAAAAAAATAGATATACTAGACTAATATAAAATCCATCTTTAAAGTGTGTAAAAGTCGAAGGAAATATCAAATATATGACAAGTTTTGAATGAACATGCATGTTTATTTTATTTTTCCAGCAATATGGAGGAGATCATTGCAAAATTTGCCCAACTTAGTCCACAAGAAAGGGCTAAAAGGTTAGTCAAATTTTCCCACTAAATATTTATTTTTATTTATTCATGATGAATGATCTAATATCTTTTCTTGTTCTAATTGAGCAGGAAAATGGAAAGTCTTGAAGTAAGATTACTTTTATCTCCTCGTAATTCATATATTACTTTTGTTGCATCGAGTGTGTATTTGATGTAGTCTCAATTTTCAGTCATTGCTTAAAAATTTGGTCTTGTTTAAAATTTAGGCACTGAAAAAAACCTTCAAGAAATTGGATCATGATGTAAAGATACAAGATTTCCTGGGTTCAAGGTAAGTGATTGTGATTATCAAATTATCAATGTCTTCCTTGTAAATGATTTTGAAGATTCTGACCTGTTTTAATTTTCATATTGCAGCAGTCAAACAGTGGAGGTACTGTTTATTCACAAATTATCAAATGGGTTAATAATTGCTTTAGAATCTTAAGCTGGTAATTAGGCGACCAAAGTGTTATGAGTTCAATTGCCATTTGCAGGACCTAAGTCATCAAGTGAGAATCTTACAAGCTCGGCTCACAGAAATACAACAGAGACTGAGGTGCAACTTTGGAGCTTTTGGTTGTTAAAGTATTTCTTTTTCTTTTTTGTGTTATTTTATTGTTCTCTTGTTGAACTATTATTCATTCAGCCTCTACCTTACCAATTTTGTATGTGCTTTGCAATTGTTGTATACCTGTATTAATTATGAGTGTGTATGTACTTTTTTTCCTTGTTCAATTTAAATTTTTCGTATTCTTTTTTTCGTTATCATTCACATATCTTCATTCACATTTCATTAAAATTGTTTCACACAACACTATTTGACATTTATGAGACTTATAAGCATTATAGACAAACTATTCTAACCCCATGCCTTTAGCTCACACCAAATAAACACCACCCAAAAAAAAATGTGTTTGTCGAAACCTATACTTTTTTGGTACAAACTCTGTTGATTAAAACATCACCAAATTATGTCAAATACTATCATAATTTGGGCTATCATATAGTAATGTTGTTTTGAATATGTAATATCTCAGTTATTGGAGTAATCTGGAGAAGATCAATAACTTGGAACATCTTAGACAAATGGAAGACTCACTCAGGGAATCAATCAATCGATTGTCTATACAAAAGGTAATATTTGGTTTCATGCGGACTAGTCATACAATTATATCATTTTCCATCGTTCCCGTCCCCTTAGAAAATCGTTGTGATTAAGAGTTGTGAAGATGTGTGTATTGATATTTTGATTTCATCGGCAGGAAAATCTCGAAAAGCACCAACTAATGTCACTTGAATGCGTTAACCAGGTAAATAATAGTGTATTTTCCCTTTTTTCTATCTATTATTATAATTTTTGAAATAATCTTATCTGAGATGATATTGATTCATAGTTACCAGAAGGGATGACTTTACCCTTAATGATGTCTGGTCTACAAGAATCTCAACCTTTGTCTTGGCTTCTAAGTGGTGATAATCACCAGTTGATGTTACCTAGTGAACCAAAGTTTATGTCATTCAGGTGAGTTAACAAGTTGCTTTCATAGACATAAAGAGCTTTATAACAAAAGTGGATCACATTTATACGAGAGTTATACATTTTAATTAACTTTCATATTTGCTTTTTAACTAATATGTTTCATAATTCTGCTAATTTTTCAAATGTTCACTATGAAAATAAAATAAAATATATATATTGCTTATTTCATTTTATAATCTAGTTATTAATTTAATTACGTTACATAGTGACAATGGCAATAGAGATGTTGAGTGCTCAACCAACATTTCACTCCCAAGCTATTCTGGTTATACTGGGAACTGCAAACTGGAGGTGGGAAGCTCTCCACATGTCACTACTTTGGGTCAGGGTGGTGGTACCTTGAATGAGTTAAATGGAAATCCAAATCCTTACTTAAATGTGCAACAGTGTCAGCAGTTTGCATACCCTCCTCCACAAGATCTCGAGGAAGTGAAACATCACCAACCAATCAATATCAAATCAAATAACGTGGACTACCAAGTTAATAACAATTATGATCTGCCAAAATCACTATTTGAAAATGGACACCAGTTCTGGAATTCTACAAATGACTCTTGTGGTATTGCTATGTATAACGAGAATGGTTTCCATAGGGTAAGCATGTAG
Protein sequence

>MS.gene58422.t1

MGRVKLKIKKLESTSNRHVTYSKRKSGILKKAKELSILCDIDILLLMFSPTGKPTLLQGERSNMEEIIAKFAQLSPQERAKRKMESLEALKKTFKKLDHDVKIQDFLGSSSQTVEDLSHQVRILQARLTEIQQRLSYWSNLEKINNLEHLRQMEDSLRESINRLSIQKENLEKHQLMSLECVNQLPEGMTLPLMMSGLQESQPLSWLLSGDNHQLMLPSEPKFMSFSDNGNRDVECSTNISLPSYSGYTGNCKLEVGSSPHVTTLGQGGGTLNELNGNPNPYLNVQQCQQFAYPPPQDLEEVKHHQPINIKSNNVDYQVNNNYDLPKSLFENGHQFWNSTNDSCGIAMYNENGFHRVSM