AlfalfaGEDB | MS.gene58550

M. sativa cultivar XinJiangDaYe / MS.gene58550

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58550.t1	XP_003613488.1	71.4	241	67	1	1	241	1	239	3.60E-77	298.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58550.t1	Q69WS3	71.4	147	39	2	98	241	110	256	5.5e-50	199.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58550.t1	G7K714	71.4	241	67	1	1	241	1	239	2.6e-77	298.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene58550.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58550.t1	MTR_5g037250	82.158	241	41	1	1	241	1	239	9.91e-145	404
MS.gene58550.t1	MTR_8g062240	60.377	265	69	7	5	241	22	278	9.13e-94	276
MS.gene58550.t1	MTR_8g012290	79.121	91	19	0	105	195	282	372	8.65e-45	157
MS.gene58550.t1	MTR_8g012290	79.121	91	19	0	105	195	282	372	8.97e-45	157
MS.gene58550.t1	MTR_7g053410	65.546	119	37	1	77	195	284	398	2.95e-44	155
MS.gene58550.t1	MTR_7g053410	65.546	119	37	1	77	195	284	398	4.86e-44	155
MS.gene58550.t1	MTR_3g116770	61.654	133	40	3	74	196	148	279	1.60e-42	149
MS.gene58550.t1	MTR_3g116770	61.654	133	40	3	74	196	148	279	1.65e-42	149
MS.gene58550.t1	MTR_4g070320	68.421	114	29	3	90	202	125	232	1.72e-41	145
MS.gene58550.t1	MTR_5g048860	58.779	131	50	3	93	220	343	472	3.49e-40	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58550.t1	AT1G59640	72.781	169	43	2	76	241	96	264	8.23e-81	243
MS.gene58550.t1	AT1G59640	64.024	164	52	4	69	225	88	251	7.61e-57	184
MS.gene58550.t1	AT5G62610	56.667	180	64	4	75	241	103	281	1.67e-56	182
MS.gene58550.t1	AT5G62610	56.667	180	64	4	75	241	103	281	1.67e-56	182
MS.gene58550.t1	AT3G23690	58.710	155	50	4	47	187	110	264	7.45e-46	157
MS.gene58550.t1	AT1G68920	57.823	147	59	3	76	220	264	409	4.45e-44	154
MS.gene58550.t1	AT1G68920	57.823	147	59	3	76	220	265	410	4.52e-44	154
MS.gene58550.t1	AT1G68920	57.823	147	59	3	76	220	265	410	4.52e-44	154
MS.gene58550.t1	AT1G68920	57.823	147	59	3	76	220	265	410	4.52e-44	154
MS.gene58550.t1	AT3G07340	59.459	148	40	5	40	187	204	331	6.14e-44	153

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Find 67 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACTGGAACCATGGC+CGG	0.515671	5.3:-25526436	MS.gene58550:CDS
AAACAGTTCAGGAAAGCATG+CGG	0.691753	5.3:-25525419	MS.gene58550:CDS
AAAGGAGAAGAAAACAGTTC+AGG	0.396640	5.3:-25525430	MS.gene58550:CDS
AAATGCGTTTCAACCGGCAA+TGG	0.555059	5.3:-25526380	MS.gene58550:CDS
AACCATACCTTGTTGCAACC+AGG	0.489186	5.3:+25524469	None:intergenic
AACGCCTCAATACCAGGAGT+TGG	0.584696	5.3:+25524032	None:intergenic
AAGACATCGGGGAACAGTAA+AGG	0.547759	5.3:-25525448	MS.gene58550:CDS
AAGACCAACTCCTGGTATTG+AGG	0.535318	5.3:-25524036	MS.gene58550:CDS
ACACTGATGGACAGGCTAGA+AGG	0.430987	5.3:-25524534	MS.gene58550:intron
ACATCCATGTTCGAGCCAGA+AGG	0.584719	5.3:-25525360	MS.gene58550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAGCTTCTAACCAAAC+TGG	+	chr5.3:25526191-25526210	None:intergenic	30.0%
AAACTCTAAATTACCAAGGT+CGG	-	chr5.3:25524379-25524398	MS.gene58550:intron	30.0%
AGTCAAACTCTAAATTACCA+AGG	-	chr5.3:25524375-25524394	MS.gene58550:intron	30.0%
ATTCTCATAAGTCTTTAAGC+CGG	+	chr5.3:25523935-25523954	None:intergenic	30.0%
CAAGTTTCATGTTCTTAACT+GGG	-	chr5.3:25525314-25525333	MS.gene58550:intron	30.0%
CTTTGTGAGAATTAGAAAGT+AGG	+	chr5.3:25524761-25524780	None:intergenic	30.0%
GATTAAGATGTTTATCCAAG+TGG	-	chr5.3:25524312-25524331	MS.gene58550:intron	30.0%
GCATTCAACGTAAAAGAATT+AGG	+	chr5.3:25526077-25526096	None:intergenic	30.0%
TAACCAAGTAGTTAAAGGTT+AGG	-	chr5.3:25523862-25523881	MS.gene58550:intron	30.0%
TACTCATTAAAACTTCCACT+TGG	+	chr5.3:25524330-25524349	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	25523516	25526558	25523516	ID=MS.gene58550
chr5.3	mRNA	25523516	25526558	25523516	ID=MS.gene58550.t1;Parent=MS.gene58550
chr5.3	exon	25526352	25526558	25526352	ID=MS.gene58550.t1.exon1;Parent=MS.gene58550.t1
chr5.3	CDS	25526352	25526558	25526352	ID=cds.MS.gene58550.t1;Parent=MS.gene58550.t1
chr5.3	exon	25525324	25525506	25525324	ID=MS.gene58550.t1.exon2;Parent=MS.gene58550.t1
chr5.3	CDS	25525324	25525506	25525324	ID=cds.MS.gene58550.t1;Parent=MS.gene58550.t1
chr5.3	exon	25524477	25524542	25524477	ID=MS.gene58550.t1.exon3;Parent=MS.gene58550.t1
chr5.3	CDS	25524477	25524542	25524477	ID=cds.MS.gene58550.t1;Parent=MS.gene58550.t1
chr5.3	exon	25524184	25524252	25524184	ID=MS.gene58550.t1.exon4;Parent=MS.gene58550.t1
chr5.3	CDS	25524184	25524252	25524184	ID=cds.MS.gene58550.t1;Parent=MS.gene58550.t1
chr5.3	exon	25524019	25524090	25524019	ID=MS.gene58550.t1.exon5;Parent=MS.gene58550.t1
chr5.3	CDS	25524019	25524090	25524019	ID=cds.MS.gene58550.t1;Parent=MS.gene58550.t1
chr5.3	exon	25523516	25523644	25523516	ID=MS.gene58550.t1.exon6;Parent=MS.gene58550.t1
chr5.3	CDS	25523516	25523644	25523516	ID=cds.MS.gene58550.t1;Parent=MS.gene58550.t1

Gene Sequence

>MS.gene58550

ATGGATCCAGCGTATCATCTCCCGGAGATCTGGCAGTCCAGAAGACCGCAGTTTAGCCCAGCTTCGGAGATTTTCCAATGGAAGATCTCAGTACTGAACAAAAACCACTGGAACCATGGCCGGAAAAGTAGAATAGAGACGAAGGAAAATTCAGTTAAATGCGTTTCAACCGGCAATGGTGGTGGCCGTGGCAATTCCAACAACGTGGTAATTGCCCACTCAATTATGCTATCTTTTTTTTGAACAACTCAATTATGTTATCTTAATTACGAAGGAAAAAATATATATTGGGTATGAATTTAGTTCTACTAATTGGTCGTATAGTTAATGAGTAATTTTGTTGTATTAACCAAGTAGTTAAAGGTTAGGTTCAACTCTTAAGGTGTGTTGGAATTTCTTTATTTGAGTTTTTTTTACCGGCTTAAAGACTTATGAGAATGTTTGAGAGAGTTTTTAAAACAACTTACGGCCTGTTTGGATAAACAACTTATTTGCACAAGCACTTAAGTATAAGCTTGCATAAGCTATTTTCATAGCAACTTGTTTTTGTATATGTGGTAAGTTGTTTTTGTAAGCTATATTGGAAAATTTATGAAAATTGTCAGAAGTTGTTTCATGGTAGATAAGTTCAAATAAGTCAATCCAAACAAGCCCATATTAAATATTAAAAAGTTGTTCTATAAGCTCTCTATGTGATAGCAACTATGAAAACAACTTACACCTTACATGAAAATAGCTTTATTTTATTTTTATTATGGAAATTACTTATACATAAGCACTTATGTGATAAGCGTTTGATTAAGATGTTTATCCAAGTGGAAGTTTTAATGAGTATTAGTTAAAATCGAATAGCAATCCTAGTCAAACTCTAAATTACCAAGGTCGGTTTACTTTTGAAAACAAGAGTTCAGACTCAAAATAATGTGCAATTTCGTATTACAATATGGAGAATGGATCTGCCTATTGCGGATTACTATTTTATTTTTATTTTTTTTTTGTTGGAATTCAAGCATTGTTATTGGAGTAGAGGTAGTGAATTTGGGTTTGTGCAGAATGATGATGGTGGTAGTGGTGATGGAAAAAGGAGTAAGACATCGGGGAACAGTAAAGGAGAAGAAAACAGTTCAGGAAAGCATGCGGAAGAAACTAATGTTGAGCCACATCCTAAGAAAGACTACATCCATGTTCGAGCCAGAAGGGGTCAAGCTACTGATAGCCATAGTCTTGCAGAAAGAGTATTTTCCTACTTTCTAATTCTCACAAAGTGTATTTTTATATGGTGATGAATTTCTTATGTTTAGATAATGTTGATCCTGAAGGATATTGCTTGCTTGAGTTGCTTTTGTCTTTTCAATATTACTTTTTTTGACAACTTGGAATCAGTAGGCAGCATTTGTCAATAAGTTAAGAACTTTAAAGACCAAATTCTATACTCCCTCTGGTCTTAAATATAAGAAGAAGAAGAAAAATATTCATATTTAATAGTCTCAATTCCCAAATAGAAACACAATTTACTACTTTTACTATATTTAATGATTTCCTTCTCAAAAACAATTCCTAAATTTATATAGGTTTTATTTTATAATCATGATGCTATTTATTCTCATCGAACAAGTTGTTATAAATGAGAACATACATATTTTTTATGAGACTAAGAAGATTAGGTGCACTCGACTAAGTTTCTTATTATGAATGTATGAACTATGAAGTTAAGTACCTTTTGTTTATAATCGAGACCGAATGGTGGTTTTGCTTAATGGTTGTTTTAGTTTTAGGTAACTTTTTGTGCATTTATTTGCAAGTTTCATGTTCTTAACTGGGATAATTGTAATACTGAGGCTGACACACTGACTCGGCTATGGTACAAGTCACGCTATCTGAAAATTTTCTAAAAATGTTGTGTTAAGAAAATGAAACTGTTACGTAAATGGGAGGTAATATTGATTTATTGGATATTTTGCAATCAGTGTTATTGGCTTAGTTTTCTAATGAATATTGATGAACACTGATGGACAGGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAACTCTTCAGGATTTAGTCCCTGGTTGCAACAAGGTATGGTTAACTTAGTGACTGTTTAGTTTTGTCTCTGCTCTCGCCGCATTTTTTATGTGTATAACTAAGAAGAACAAAACAAGCATAAAGACAAGAATACAATGTGTTACTTTTCATATGAAAATGAAAAGAACAAAATATCAGTAAACATTTGTGTTTATAAATCTCTGATAAGATGAGCTGAAAATATTCCTTATGGCTTGATTTGTTTTTAATGTATTCAGGTTATTGGAAAAGCATTGGTCCTTGATGAGATAATCAATTATATCCAATCACTTCACCACCAAGTAGAGGTGTGAATTATCTATCTGATGTTATCCTTTTTTCTAAAAATTTAATGTTTTATAGTGTATTTCTGAGTCTTCTATTTTTAATTTACATCACAGTTTTTGTCGATGAAGCTTGAAGCAGTAAATTCAAGACCAACTCCTGGTATTGAGGCGTTTCCTCTTAAAACTGTAAGTATTTAACAGCATCCTAATTCTTTTACGTTGAATGCTATATATTCTAGAAGTTGGTTTTATCTGCTTTATTCTATATAATGTCCTTTGGTGAGATGAGTATAACTGCCCTTTTTTATCGCAAGGATACCAGTTTGGTTAGAAGCTTTTTTTGTGCACACTATTTGATTAGTAGATAGTAGCAGCAGGTAGGAAACATAATAAATCTATTTGAGGTTTTCCGTTAAAAATTTGAGGACTAAAATCACCCAATAATCATTGAGTGACCAAAATCAGACCTAGTCCAAATTAAAGAGTTTAAAGTGCAATTAAGTTTTCACCTTTTTAGCTAAATCTATCTTCATTCTCATGAACATCAACTTGTATTCCAGTTTGATCAGCAAACATATGATACAACCACCATACCATTCACTTCACAAGCTACAAGAGAGTACAGCCGAGGTGCATCACCAGAGTGGTTACATATGCAGCTGGGAGGTGGTTTTGAAAGAACAACATAA

Protein sequence

>MS.gene58550.t1

MDPAYHLPEIWQSRRPQFSPASEIFQWKISVLNKNHWNHGRKSRIETKENSVKCVSTGNGGGRGNSNNVNDDGGSGDGKRSKTSGNSKGEENSSGKHAEETNVEPHPKKDYIHVRARRGQATDSHSLAERARREKISERMKTLQDLVPGCNKVIGKALVLDEIINYIQSLHHQVEFLSMKLEAVNSRPTPGIEAFPLKTFDQQTYDTTTIPFTSQATREYSRGASPEWLHMQLGGGFERTT