AlfalfaGEDB | MS.gene58574

M. sativa cultivar XinJiangDaYe / MS.gene58574

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58574.t1	AES96405.2	69.4	85	26	0	1	85	1	85	5.60E-25	123.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58574.t1	Q9XIK6	41.8	67	39	0	16	82	164	230	5.2e-11	68.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58574.t1	G7K6X3	69.4	85	26	0	1	85	1	85	4.1e-25	123.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene58574.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58574.t1	MTR_5g036790	69.412	85	26	0	1	85	1	85	1.50e-39	125
MS.gene58574.t1	MTR_5g036820	63.095	84	29	1	1	82	1	84	1.38e-32	108
MS.gene58574.t1	MTR_7g013525	48.148	81	41	1	6	85	1	81	4.76e-16	66.6
MS.gene58574.t1	MTR_6g008995	39.759	83	42	1	3	85	112	186	6.64e-15	68.2
MS.gene58574.t1	MTR_6g008995	39.759	83	42	1	3	85	55	129	6.72e-15	68.2
MS.gene58574.t1	MTR_7g013410	45.588	68	37	0	15	82	74	141	8.85e-15	64.7
MS.gene58574.t1	MTR_6g008970	41.429	70	41	0	13	82	142	211	1.25e-14	67.4
MS.gene58574.t1	MTR_5g036670	38.554	83	44	1	2	84	118	193	2.26e-13	63.9
MS.gene58574.t1	MTR_8g062700	40.299	67	40	0	16	82	124	190	5.13e-11	57.4
MS.gene58574.t1	MTR_8g062700	40.299	67	40	0	16	82	124	190	5.13e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58574.t1	AT1G10450	41.791	67	39	0	16	82	164	230	9.72e-15	67.8
MS.gene58574.t1	AT1G10450	41.791	67	39	0	16	82	113	179	1.02e-14	67.8
MS.gene58574.t1	AT1G10450	41.791	67	39	0	16	82	113	179	1.02e-14	67.8
MS.gene58574.t1	AT3G01320	40.000	70	42	0	13	82	135	204	2.71e-13	63.9
MS.gene58574.t1	AT3G01320	40.000	70	42	0	13	82	135	204	2.74e-13	63.5
MS.gene58574.t1	AT5G15020	41.429	70	41	0	13	82	129	198	6.85e-13	62.8
MS.gene58574.t1	AT5G15020	41.429	70	41	0	13	82	129	198	6.99e-13	62.4
MS.gene58574.t1	AT1G70060	37.805	82	50	1	1	82	83	163	7.26e-13	62.4
MS.gene58574.t1	AT1G70060	37.805	82	50	1	1	82	83	163	7.26e-13	62.4
MS.gene58574.t1	AT1G24190	43.284	67	38	0	16	82	96	162	2.41e-12	61.2
MS.gene58574.t1	AT1G24190	43.284	67	38	0	16	82	96	162	2.66e-12	60.8
MS.gene58574.t1	AT1G24190	43.284	67	38	0	16	82	96	162	2.66e-12	60.8
MS.gene58574.t1	AT1G59890	37.313	67	42	0	16	82	125	191	9.32e-12	59.3
MS.gene58574.t1	AT1G59890	37.313	67	42	0	16	82	125	191	9.32e-12	59.3
MS.gene58574.t1	AT1G59890	37.313	67	42	0	16	82	125	191	9.88e-12	59.3
MS.gene58574.t1	AT1G59890	37.313	67	42	0	16	82	125	191	1.02e-11	59.3
MS.gene58574.t1	AT1G70030	36.000	75	47	1	11	85	87	160	9.98e-11	54.7

Find 18 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGTGGTGGATTATGAATA+TGG	0.290334	5.3:-25032480	MS.gene58574:CDS
AGAGTCTTGATGATGTCATT+TGG	0.357491	5.3:-25032251	MS.gene58574:CDS
GAGTCTTGATGATGTCATTT+GGG	0.414208	5.3:-25032250	MS.gene58574:CDS
GGCCATGATGATCTTATTGA+TGG	0.460349	5.3:-25032099	MS.gene58574:CDS
GCCATGATGATCTTATTGAT+GGG	0.565009	5.3:-25032098	MS.gene58574:CDS
GAGATATATGATGAAGGTGA+AGG	0.585513	5.3:-25032454	MS.gene58574:intron
TTTATTGATTTCCTTCCGCA+AGG	0.586502	5.3:-25032075	MS.gene58574:CDS
ATTCATAATCCACCACTGGC+CGG	0.587375	5.3:+25032484	None:intergenic
TGAAAAGAACATATGCTGAC+CGG	0.588434	5.3:-25032503	MS.gene58574:CDS
ATATGCTGACCGGCCAGTGG+TGG	0.590440	5.3:-25032493	MS.gene58574:CDS
ATCACTAGCCACCTTGCGGA+AGG	0.593474	5.3:+25032064	None:intergenic
ACCCATCAATAAGATCATCA+TGG	0.602513	5.3:+25032097	None:intergenic
CCATATTCATAATCCACCAC+TGG	0.614448	5.3:+25032480	None:intergenic
AACATATGCTGACCGGCCAG+TGG	0.637326	5.3:-25032496	MS.gene58574:CDS
ATTGATTTCCTTCCGCAAGG+TGG	0.643769	5.3:-25032072	MS.gene58574:CDS
TGGTTTGAGATATATGATGA+AGG	0.650047	5.3:-25032460	MS.gene58574:CDS
ATATATCACTAGCCACCTTG+CGG	0.662180	5.3:+25032060	None:intergenic
TCTTGATGATGTCATTTGGG+CGG	0.663888	5.3:-25032247	MS.gene58574:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTATGATTTTAATTCTTC+AGG	-	chr5.3:25032448-25032467	MS.gene58574:intron	15.0%
!!	AAGAGGAAAGAAAAATAAAA+AGG	+	chr5.3:25032412-25032431	None:intergenic	20.0%
	GGTGAAGGTAAGTTATATTA+TGG	-	chr5.3:25032148-25032167	MS.gene58574:intron	30.0%
!	TATGTTCTTTTCATCTTCTG+TGG	+	chr5.3:25032077-25032096	None:intergenic	30.0%
!	TGGTTTGAGATATATGATGA+AGG	-	chr5.3:25032127-25032146	MS.gene58574:intron	30.0%
!!	TAAAAAGAGTATCACACAAG+AGG	+	chr5.3:25032429-25032448	None:intergenic	30.0%
!!!	CAGGTTTCTTTACTTTTTGA+AGG	-	chr5.3:25032467-25032486	MS.gene58574:CDS	30.0%
	ACCCATCAATAAGATCATCA+TGG	+	chr5.3:25032493-25032512	None:intergenic	35.0%
	GAGATATATGATGAAGGTGA+AGG	-	chr5.3:25032133-25032152	MS.gene58574:intron	35.0%
	GCCATGATGATCTTATTGAT+GGG	-	chr5.3:25032489-25032508	MS.gene58574:CDS	35.0%
	TGAAAAGAACATATGCTGAC+CGG	-	chr5.3:25032084-25032103	MS.gene58574:CDS	35.0%
!!	AGAGTCTTGATGATGTCATT+TGG	-	chr5.3:25032336-25032355	MS.gene58574:intron	35.0%
!!	GAGTCTTGATGATGTCATTT+GGG	-	chr5.3:25032337-25032356	MS.gene58574:intron	35.0%
!!	TTTATTGATTTCCTTCCGCA+AGG	-	chr5.3:25032512-25032531	MS.gene58574:CDS	35.0%
	CCATATTCATAATCCACCAC+TGG	+	chr5.3:25032110-25032129	None:intergenic	40.0%
	GGCCATGATGATCTTATTGA+TGG	-	chr5.3:25032488-25032507	MS.gene58574:CDS	40.0%
!!	CCAGTGGTGGATTATGAATA+TGG	-	chr5.3:25032107-25032126	MS.gene58574:CDS	40.0%
!!	TCTTGATGATGTCATTTGGG+CGG	-	chr5.3:25032340-25032359	MS.gene58574:intron	40.0%
	ATTCATAATCCACCACTGGC+CGG	+	chr5.3:25032106-25032125	None:intergenic	45.0%
!	ATTGATTTCCTTCCGCAAGG+TGG	-	chr5.3:25032515-25032534	MS.gene58574:CDS	45.0%
	AACATATGCTGACCGGCCAG+TGG	-	chr5.3:25032091-25032110	MS.gene58574:CDS	55.0%
	ATATGCTGACCGGCCAGTGG+TGG	-	chr5.3:25032094-25032113	MS.gene58574:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	25032068	25032541	25032068	ID=MS.gene58574
chr5.3	mRNA	25032068	25032541	25032068	ID=MS.gene58574.t1;Parent=MS.gene58574
chr5.3	exon	25032455	25032541	25032455	ID=MS.gene58574.t1.exon1;Parent=MS.gene58574.t1
chr5.3	CDS	25032455	25032541	25032455	ID=cds.MS.gene58574.t1;Parent=MS.gene58574.t1
chr5.3	exon	25032248	25032346	25032248	ID=MS.gene58574.t1.exon2;Parent=MS.gene58574.t1
chr5.3	CDS	25032248	25032346	25032248	ID=cds.MS.gene58574.t1;Parent=MS.gene58574.t1
chr5.3	exon	25032068	25032139	25032068	ID=MS.gene58574.t1.exon3;Parent=MS.gene58574.t1
chr5.3	CDS	25032068	25032139	25032068	ID=cds.MS.gene58574.t1;Parent=MS.gene58574.t1

Gene Sequence

>MS.gene58574

ATGAAACCACAGAAGATGAAAAGAACATATGCTGACCGGCCAGTGGTGGATTATGAATATGGTTTGAGATATATGATGAAGGTGAAGGTAAGTTATATTATGGATTCGATTCTTTCACACATATATGTTTTCAATGTAGAATTAATCAATGAAATATTTCAGTTTTGACGAGTCTAATAGATGTTTTTCATGCAGACTCGATTTCAAGATGCTCGTCATGTGTATTCGTCGTTTCAAGACATGCTGAAAATGTACAAAGAAAGAGAAAAGAGTCTTGATGATGTCATTTGGGCGGTATATGTTGTGTTGTATTGTTTATGTCACACATAGTAAAATATATGCCTTTTTATTTTTCTTTCCTCTTGTGTGATACTCTTTTTAATTATGATTTTAATTCTTCAGGTTTCTTTACTTTTTGAAGGCCATGATGATCTTATTGATGGGTTTATTGATTTCCTTCCGCAAGGTGGCTAG

Protein sequence

>MS.gene58574.t1

MKPQKMKRTYADRPVVDYEYGLRYMMKVKTRFQDARHVYSSFQDMLKMYKEREKSLDDVIWAVSLLFEGHDDLIDGFIDFLPQGG