AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58658

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58658.t1 MTR_2g437200 70.915 306 85 2 1 304 1 304 1.58e-146 419
MS.gene58658.t1 MTR_2g437240 69.000 300 89 2 1 297 1 299 2.57e-144 414
MS.gene58658.t1 MTR_2g438020 74.436 266 68 0 29 294 70 335 2.55e-138 401
MS.gene58658.t1 MTR_2g437960 60.674 267 68 2 30 294 53 284 9.69e-104 310
MS.gene58658.t1 MTR_2g437960 60.674 267 68 2 30 294 53 284 1.29e-103 310
MS.gene58658.t1 MTR_2g437150 55.749 287 84 4 21 299 42 293 2.52e-98 296
MS.gene58658.t1 MTR_2g437180 58.065 279 72 4 21 294 42 280 2.83e-98 296
MS.gene58658.t1 MTR_2g437170 55.749 287 84 4 21 299 42 293 4.24e-98 296
MS.gene58658.t1 MTR_2g437120 58.273 278 78 3 22 297 43 284 1.91e-97 294
MS.gene58658.t1 MTR_2g437100 54.545 286 83 4 21 294 43 293 9.93e-92 280
MS.gene58658.t1 MTR_2g437160 52.542 295 96 5 21 309 50 306 1.33e-89 274
MS.gene58658.t1 MTR_2g076320 54.412 272 87 2 25 294 45 281 1.03e-87 269
MS.gene58658.t1 MTR_2g437990 53.676 272 88 3 25 294 45 280 4.04e-85 263
MS.gene58658.t1 MTR_2g437260 48.161 299 109 6 1 291 1 261 2.71e-84 260
MS.gene58658.t1 MTR_2g437130 50.545 275 98 3 25 299 38 274 4.60e-79 246
MS.gene58658.t1 MTR_2g438010 50.741 270 96 4 27 294 67 301 9.93e-79 246
MS.gene58658.t1 MTR_2g437080 42.086 278 121 3 19 294 29 268 8.74e-71 225
MS.gene58658.t1 MTR_2g437060 42.086 278 121 3 19 294 44 283 3.87e-68 218
MS.gene58658.t1 MTR_2g437030 41.667 288 126 4 19 304 115 362 4.03e-68 220
MS.gene58658.t1 MTR_4g119550 41.328 271 120 3 25 294 46 278 8.16e-68 218
MS.gene58658.t1 MTR_2g437020 43.011 279 117 4 20 294 29 269 1.05e-66 214
MS.gene58658.t1 MTR_2g436400 40.942 276 121 5 22 294 39 275 6.28e-65 209
MS.gene58658.t1 MTR_2g436440 40.625 288 131 3 9 294 25 274 4.96e-64 207
MS.gene58658.t1 MTR_2g437040 41.522 289 124 6 8 294 23 268 4.46e-62 202
MS.gene58658.t1 MTR_4g120380 39.716 282 130 3 24 305 38 279 8.01e-59 194
MS.gene58658.t1 MTR_4g120380 39.716 282 130 3 24 305 38 279 6.91e-58 192
MS.gene58658.t1 MTR_2g436460 40.809 272 122 5 24 294 41 274 2.75e-55 184
MS.gene58658.t1 MTR_4g119580 39.163 263 122 2 32 294 46 270 3.57e-52 176
MS.gene58658.t1 MTR_2g019810 37.729 273 119 6 29 294 30 258 4.41e-49 168
MS.gene58658.t1 MTR_2g019840 35.766 274 133 6 24 294 37 270 1.14e-47 165
MS.gene58658.t1 MTR_2g437940 38.372 344 163 12 21 328 42 372 7.06e-46 160
MS.gene58658.t1 MTR_4g119570 41.104 163 91 2 144 306 15 172 1.86e-36 131
MS.gene58658.t1 MTR_2g436370 41.618 173 64 3 21 191 37 174 9.28e-36 129
MS.gene58658.t1 MTR_3g450500 30.612 294 160 8 32 320 40 294 5.44e-34 128
MS.gene58658.t1 MTR_4g007490 30.877 285 154 7 32 312 39 284 1.53e-33 127
MS.gene58658.t1 MTR_4g049400 50.435 115 51 2 21 129 37 151 3.84e-28 109
MS.gene58658.t1 MTR_2g437160 69.565 69 21 0 226 294 134 202 3.85e-24 100
MS.gene58658.t1 MTR_3g085240 34.450 209 92 5 1 172 21 221 4.07e-21 91.3
MS.gene58658.t1 MTR_8g080990 26.953 256 145 5 61 314 74 289 1.66e-16 80.1
MS.gene58658.t1 MTR_8g081000 25.581 258 150 5 59 314 85 302 8.83e-16 77.8
MS.gene58658.t1 MTR_8g081000 25.581 258 150 5 59 314 85 302 1.00e-15 77.8
MS.gene58658.t1 MTR_8g081000 25.581 258 150 5 59 314 85 302 1.01e-15 77.8
MS.gene58658.t1 MTR_2g436380 58.929 56 23 0 239 294 1 56 1.53e-15 76.3
MS.gene58658.t1 MTR_2g437090 49.180 61 31 0 239 299 1 61 5.10e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58658.t1 AT5G07900 26.821 302 170 6 23 311 51 314 7.34e-34 129
MS.gene58658.t1 AT1G21150 30.153 262 146 2 32 291 57 283 2.77e-30 119
MS.gene58658.t1 AT1G21150 29.811 265 149 2 29 291 18 247 5.50e-30 117
MS.gene58658.t1 AT1G21150 30.153 262 146 2 32 291 121 347 6.46e-30 119
MS.gene58658.t1 AT3G46950 28.402 338 182 11 6 329 26 317 4.17e-26 108
MS.gene58658.t1 AT1G62120 27.820 266 155 3 30 294 55 284 7.78e-24 101
MS.gene58658.t1 AT1G61960 28.302 265 150 4 32 294 53 279 2.15e-23 100
MS.gene58658.t1 AT1G62110 25.965 285 163 5 32 311 53 294 1.07e-22 98.6
MS.gene58658.t1 AT1G61990 27.055 292 163 4 8 294 25 271 2.06e-22 97.4
MS.gene58658.t1 AT1G61990 27.055 292 163 4 8 294 25 271 2.06e-22 97.4
MS.gene58658.t1 AT1G62150 26.136 264 158 3 32 294 59 286 2.15e-22 97.8
MS.gene58658.t1 AT1G62085 27.273 264 155 4 32 294 56 283 2.57e-22 97.4
MS.gene58658.t1 AT1G62085 27.273 264 155 4 32 294 56 283 2.57e-22 97.4
MS.gene58658.t1 AT1G61980 27.820 266 149 5 32 294 53 278 5.20e-22 96.3
MS.gene58658.t1 AT1G61980 27.820 266 149 5 32 294 53 278 5.20e-22 96.3
MS.gene58658.t1 AT1G61980 27.820 266 149 5 32 294 53 278 5.20e-22 96.3
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT1G61970 27.817 284 157 9 32 311 53 292 2.25e-20 91.7
MS.gene58658.t1 AT5G23930 26.791 321 163 9 32 326 56 330 1.11e-16 80.9
MS.gene58658.t1 AT5G64950 21.695 295 189 4 24 314 32 288 8.62e-13 68.9
MS.gene58658.t1 AT1G62010 25.000 256 146 4 64 318 71 281 2.83e-11 64.7

Find 57 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCAAGGCCATCTATATT+TGG 0.148576 2.3:-53680745 MS.gene58658:CDS
ATCAGTTTGATGACTGATTT+TGG 0.150667 2.3:-53680799 MS.gene58658:CDS
GATCCGTCATTGACTACTTT+TGG 0.212291 2.3:-53680673 MS.gene58658:CDS
TGGAGGAAGTAAAGAGTTTA+GGG 0.240494 2.3:-53680699 MS.gene58658:CDS
GACATTGTCCGCATGGTTAA+AGG 0.256602 2.3:-53681084 MS.gene58658:CDS
TTTATCTGTCGTAATGCTTT+TGG 0.259818 2.3:-53681414 MS.gene58658:CDS
TTGGAGGAAGTAAAGAGTTT+AGG 0.267899 2.3:-53680700 MS.gene58658:CDS
GAAGCTTTGCGTGCGGATTC+AGG 0.269165 2.3:+53681294 None:intergenic
AAACCGTTATTCGAGTATTT+AGG 0.278181 2.3:-53680564 MS.gene58658:intron
AACTGATTTGATTAAATCAT+TGG 0.289242 2.3:-53680719 MS.gene58658:CDS
ATTTGATGTCTTTAAGAAAT+GGG 0.334222 2.3:-53680599 MS.gene58658:CDS
GAAGAAGTTGATAACTGATT+CGG 0.343473 2.3:+53681239 None:intergenic
TCCTACCTCGTCGACAAATT+CGG 0.356172 2.3:-53681324 MS.gene58658:CDS
AATTTGATGTCTTTAAGAAA+TGG 0.359065 2.3:-53680600 MS.gene58658:CDS
AAGCTAATGTGTAAAATTCC+AGG 0.363279 2.3:+53680952 None:intergenic
GTCAGATTCAGAAGCACCTT+TGG 0.364937 2.3:+53681104 None:intergenic
AGATCAAAATCTTATACATT+TGG 0.374741 2.3:+53680195 None:intergenic
AAGAAGTTGATAACTGATTC+GGG 0.391290 2.3:+53681240 None:intergenic
ATCAGAAGAAGAAGCACCTT+TGG 0.395286 2.3:+53680999 None:intergenic
TCAGTTGAACCAAATATAGA+TGG 0.441181 2.3:+53680736 None:intergenic
TCAAAATCTTATACATTTGG+TGG 0.453156 2.3:+53680198 None:intergenic
ACATACGCAATATCATCAAA+AGG 0.472800 2.3:-53681191 MS.gene58658:CDS
CAAATACAACGGAGAACAAT+GGG 0.473276 2.3:-53680228 MS.gene58658:CDS
AATATCATCAAAAGGGTACC+AGG 0.494005 2.3:-53681183 MS.gene58658:CDS
TACTCGAATAACGGTTTCAT+CGG 0.500961 2.3:+53680570 None:intergenic
TTTGATGTCTTTAAGAAATG+GGG 0.510130 2.3:-53680598 MS.gene58658:CDS
AGCTTAATTGCAAGAACTGA+GGG 0.512098 2.3:+53681058 None:intergenic
ATACCTAAATACTCGAATAA+CGG 0.521907 2.3:+53680561 None:intergenic
AGCTAATGTGTAAAATTCCA+GGG 0.541017 2.3:+53680953 None:intergenic
TTATCTGTTGAAGCTATATG+AGG 0.544556 2.3:-53680266 MS.gene58658:CDS
GTGAAGTGGTAGTAGTGGTA+AGG 0.547397 2.3:+53681361 None:intergenic
CATACGCAATATCATCAAAA+GGG 0.549145 2.3:-53681190 MS.gene58658:CDS
AACTGATATTAGCCACCGTA+AGG 0.557576 2.3:+53680815 None:intergenic
TCGCTAATTTGCTTCACTCA+AGG 0.562969 2.3:-53680759 MS.gene58658:CDS
TTTCAGCTTTAAGGAGGAGT+CGG 0.563069 2.3:-53680290 MS.gene58658:intron
TGATTTGATTAAATCATTGG+AGG 0.568194 2.3:-53680716 MS.gene58658:CDS
CTCTTTGGAAGCTTTGCGTG+CGG 0.569628 2.3:+53681287 None:intergenic
GCAAATACAACGGAGAACAA+TGG 0.572984 2.3:-53680229 MS.gene58658:CDS
TGAATCTGACATTGTCCGCA+TGG 0.573592 2.3:-53681091 MS.gene58658:CDS
GCTGCTTTGAAAGCCAAGAA+AGG 0.575078 2.3:-53680649 MS.gene58658:CDS
TAATCGTCAGCGCCTTACGG+TGG 0.584026 2.3:-53680827 MS.gene58658:CDS
GTATTGCAGGAAAGAATCCC+TGG 0.593025 2.3:+53681165 None:intergenic
CGAGGTAGGACACAGCGAAA+GGG 0.593390 2.3:+53681337 None:intergenic
TCCGAATTTGTCGACGAGGT+AGG 0.594044 2.3:+53681323 None:intergenic
AAGCTTAATTGCAAGAACTG+AGG 0.598624 2.3:+53681057 None:intergenic
ACGAGGTAGGACACAGCGAA+AGG 0.604629 2.3:+53681336 None:intergenic
ATGTCTTTAAGAAATGGGGT+TGG 0.618819 2.3:-53680594 MS.gene58658:CDS
AGGGTGTGAAGTGGTAGTAG+TGG 0.620465 2.3:+53681356 None:intergenic
GCCCCAAAAGTAGTCAATGA+CGG 0.622060 2.3:+53680670 None:intergenic
TCATTGTTAACTGCAAACCC+TGG 0.624716 2.3:-53680970 MS.gene58658:CDS
CACAGCGAAAGGGTGTGAAG+TGG 0.647943 2.3:+53681347 None:intergenic
ATGTCGAATTAAGCAAACAA+TGG 0.648992 2.3:+53680867 None:intergenic
AATGAAACTTGCAAATACAA+CGG 0.655557 2.3:-53680239 MS.gene58658:CDS
AAGGTTCTTTAAAACTGACA+AGG 0.658226 2.3:-53680893 MS.gene58658:CDS
ATATCATCAAAAGGGTACCA+GGG 0.689286 2.3:-53681182 MS.gene58658:CDS
AGTATCCGAATTTGTCGACG+AGG 0.717242 2.3:+53681319 None:intergenic
GAGGGTTACCTTTAACCATG+CGG 0.736609 2.3:+53681076 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTAATAAGATTAATT+TGG - chr2.3:53681106-53681125 MS.gene58658:CDS 10.0%
!! TAGTTATAGTTTACATAAAA+GGG - chr2.3:53681196-53681215 MS.gene58658:CDS 15.0%
!! TTAGTTATAGTTTACATAAA+AGG - chr2.3:53681195-53681214 MS.gene58658:CDS 15.0%
!! AGAGTAAAAATTGAAACTTT+GGG + chr2.3:53680500-53680519 None:intergenic 20.0%
!! AGGTATTAGTAAAAAACTTT+GGG - chr2.3:53680995-53681014 MS.gene58658:CDS 20.0%
!! TTCTTAACAATTCAAAAAGA+GGG + chr2.3:53680713-53680732 None:intergenic 20.0%
!!! AACTGATTTGATTAAATCAT+TGG - chr2.3:53680905-53680924 MS.gene58658:CDS 20.0%
!!! AAGGTATTAGTAAAAAACTT+TGG - chr2.3:53680994-53681013 MS.gene58658:CDS 20.0%
!!! AATTTGATGTCTTTAAGAAA+TGG - chr2.3:53681024-53681043 MS.gene58658:CDS 20.0%
!!! ATTTGATGTCTTTAAGAAAT+GGG - chr2.3:53681025-53681044 MS.gene58658:CDS 20.0%
!!! TTTCAATATTTTCTATCCAA+AGG - chr2.3:53680609-53680628 MS.gene58658:CDS 20.0%
!!! TTTTTTACTAATACCTTTCT+TGG + chr2.3:53680991-53681010 None:intergenic 20.0%
! AATGAAACTTGCAAATACAA+CGG - chr2.3:53681385-53681404 MS.gene58658:CDS 25.0%
! ATACCTAAATACTCGAATAA+CGG + chr2.3:53681066-53681085 None:intergenic 25.0%
! ATATCCTCAGAATAAGTAAA+TGG + chr2.3:53681296-53681315 None:intergenic 25.0%
! ATCAACTTCTTCAAAAATCA+TGG - chr2.3:53680396-53680415 MS.gene58658:intron 25.0%
! ATTGAAGTCAACATCAAATT+AGG + chr2.3:53681092-53681111 None:intergenic 25.0%
! CTTCTTAACAATTCAAAAAG+AGG + chr2.3:53680714-53680733 None:intergenic 25.0%
! GAGAGTAAAAATTGAAACTT+TGG + chr2.3:53680501-53680520 None:intergenic 25.0%
! TCAAAATCTTATACATTTGG+TGG + chr2.3:53681429-53681448 None:intergenic 25.0%
! TGATTTGATTAAATCATTGG+AGG - chr2.3:53680908-53680927 MS.gene58658:CDS 25.0%
!! CTCTTTTTGAATTGTTAAGA+AGG - chr2.3:53680712-53680731 MS.gene58658:CDS 25.0%
!! TTGCAATTTCTTTTGATGAA+AGG - chr2.3:53681239-53681258 MS.gene58658:CDS 25.0%
!! TTTCAATTTTTACTCTCCAA+AGG - chr2.3:53680504-53680523 MS.gene58658:intron 25.0%
!! TTTGATGTCTTTAAGAAATG+GGG - chr2.3:53681026-53681045 MS.gene58658:CDS 25.0%
!!! TAATTTGGTGATGAGATTTT+GGG - chr2.3:53681121-53681140 MS.gene58658:CDS 25.0%
!!! TTAATTTGGTGATGAGATTT+TGG - chr2.3:53681120-53681139 MS.gene58658:CDS 25.0%
AAACCGTTATTCGAGTATTT+AGG - chr2.3:53681060-53681079 MS.gene58658:CDS 30.0%
AAGCTAATGTGTAAAATTCC+AGG + chr2.3:53680675-53680694 None:intergenic 30.0%
ACATACGCAATATCATCAAA+AGG - chr2.3:53680433-53680452 MS.gene58658:intron 30.0%
AGCTAATGTGTAAAATTCCA+GGG + chr2.3:53680674-53680693 None:intergenic 30.0%
ATGTCGAATTAAGCAAACAA+TGG + chr2.3:53680760-53680779 None:intergenic 30.0%
CAAAAGAAATTGCAAAACTG+AGG + chr2.3:53681234-53681253 None:intergenic 30.0%
CATACGCAATATCATCAAAA+GGG - chr2.3:53680434-53680453 MS.gene58658:intron 30.0%
TCAGTTGAACCAAATATAGA+TGG + chr2.3:53680891-53680910 None:intergenic 30.0%
TTATCTGTTGAAGCTATATG+AGG - chr2.3:53681358-53681377 MS.gene58658:CDS 30.0%
! TGGAATTTTACACATTAGCT+TGG - chr2.3:53680674-53680693 MS.gene58658:CDS 30.0%
! TTCTTTTGATGAAAGGTTTG+CGG - chr2.3:53681246-53681265 MS.gene58658:CDS 30.0%
! TTTATCTGTCGTAATGCTTT+TGG - chr2.3:53680210-53680229 MS.gene58658:CDS 30.0%
!! AAGAAGTTGATAACTGATTC+GGG + chr2.3:53680387-53680406 None:intergenic 30.0%
!! AAGGTTCTTTAAAACTGACA+AGG - chr2.3:53680731-53680750 MS.gene58658:CDS 30.0%
!! AGATTTTGGGTCAATCAATT+GGG - chr2.3:53681134-53681153 MS.gene58658:CDS 30.0%
!! ATCAGTTTGATGACTGATTT+TGG - chr2.3:53680825-53680844 MS.gene58658:CDS 30.0%
!! CAAGTTTGTTTTCAGCTTTA+AGG - chr2.3:53681325-53681344 MS.gene58658:CDS 30.0%
!! GAAGAAGTTGATAACTGATT+CGG + chr2.3:53680388-53680407 None:intergenic 30.0%
!!! GGTTTTGAAATAAAGCTCTT+TGG + chr2.3:53680355-53680374 None:intergenic 30.0%
AAGCTTAATTGCAAGAACTG+AGG + chr2.3:53680570-53680589 None:intergenic 35.0%
ACATAAAAGGGTCATTCCAA+GGG - chr2.3:53681208-53681227 MS.gene58658:CDS 35.0%
AGCTTAATTGCAAGAACTGA+GGG + chr2.3:53680569-53680588 None:intergenic 35.0%
CAAATACAACGGAGAACAAT+GGG - chr2.3:53681396-53681415 MS.gene58658:CDS 35.0%
CACACCATTTACTTATTCTG+AGG - chr2.3:53681289-53681308 MS.gene58658:CDS 35.0%
TACATAAAAGGGTCATTCCA+AGG - chr2.3:53681207-53681226 MS.gene58658:CDS 35.0%
TACTCGAATAACGGTTTCAT+CGG + chr2.3:53681057-53681076 None:intergenic 35.0%
TGGAGGAAGTAAAGAGTTTA+GGG - chr2.3:53680925-53680944 MS.gene58658:CDS 35.0%
TTGGAGGAAGTAAAGAGTTT+AGG - chr2.3:53680924-53680943 MS.gene58658:CDS 35.0%
! ATGTCTTTAAGAAATGGGGT+TGG - chr2.3:53681030-53681049 MS.gene58658:CDS 35.0%
!! AATATCATCAAAAGGGTACC+AGG - chr2.3:53680441-53680460 MS.gene58658:intron 35.0%
!! ATATCATCAAAAGGGTACCA+GGG - chr2.3:53680442-53680461 MS.gene58658:intron 35.0%
!! ATCCGTCATTGACTACTTTT+GGG - chr2.3:53680952-53680971 MS.gene58658:CDS 35.0%
!! GAGATTTTGGGTCAATCAAT+TGG - chr2.3:53681133-53681152 MS.gene58658:CDS 35.0%
!!! GTTTGTTTTCAGCTTTAAGG+AGG - chr2.3:53681328-53681347 MS.gene58658:CDS 35.0%
AACTGATATTAGCCACCGTA+AGG + chr2.3:53680812-53680831 None:intergenic 40.0%
CACTCAAGGCCATCTATATT+TGG - chr2.3:53680879-53680898 MS.gene58658:CDS 40.0%
GCAAATACAACGGAGAACAA+TGG - chr2.3:53681395-53681414 MS.gene58658:CDS 40.0%
TCATTGTTAACTGCAAACCC+TGG - chr2.3:53680654-53680673 MS.gene58658:CDS 40.0%
TGCACATTTAGTAAGTGCCA+GGG + chr2.3:53681168-53681187 None:intergenic 40.0%
TTGGGTCAATCAATTGGGTT+GGG - chr2.3:53681139-53681158 MS.gene58658:CDS 40.0%
TTTGGGTCAATCAATTGGGT+TGG - chr2.3:53681138-53681157 MS.gene58658:CDS 40.0%
! TTTTAATCGTCAGCGCCTTA+CGG - chr2.3:53680794-53680813 MS.gene58658:CDS 40.0%
!! AATCGTTTTGTGGGTATTGC+AGG + chr2.3:53680475-53680494 None:intergenic 40.0%
!! ACTTTGGGAGAATCGTTTTG+TGG + chr2.3:53680485-53680504 None:intergenic 40.0%
!! ATCAGAAGAAGAAGCACCTT+TGG + chr2.3:53680628-53680647 None:intergenic 40.0%
!! CTTTGGGAGAATCGTTTTGT+GGG + chr2.3:53680484-53680503 None:intergenic 40.0%
!! GATCCGTCATTGACTACTTT+TGG - chr2.3:53680951-53680970 MS.gene58658:CDS 40.0%
!! TCCGTCATTGACTACTTTTG+GGG - chr2.3:53680953-53680972 MS.gene58658:CDS 40.0%
!! TCGCTAATTTGCTTCACTCA+AGG - chr2.3:53680865-53680884 MS.gene58658:CDS 40.0%
!! TTTCAGCTTTAAGGAGGAGT+CGG - chr2.3:53681334-53681353 MS.gene58658:CDS 40.0%
!!! AACTGATTCGGGTTTTTGTG+AGG + chr2.3:53680376-53680395 None:intergenic 40.0%
AATTGCAAAACTGAGGCCCT+TGG + chr2.3:53681227-53681246 None:intergenic 45.0%
AGTATCCGAATTTGTCGACG+AGG + chr2.3:53680308-53680327 None:intergenic 45.0%
GACATTGTCCGCATGGTTAA+AGG - chr2.3:53680540-53680559 MS.gene58658:intron 45.0%
GAGGGTTACCTTTAACCATG+CGG + chr2.3:53680551-53680570 None:intergenic 45.0%
GCCCCAAAAGTAGTCAATGA+CGG + chr2.3:53680957-53680976 None:intergenic 45.0%
GTATTGCAGGAAAGAATCCC+TGG + chr2.3:53680462-53680481 None:intergenic 45.0%
GTGAAGTGGTAGTAGTGGTA+AGG + chr2.3:53680266-53680285 None:intergenic 45.0%
GTGCACATTTAGTAAGTGCC+AGG + chr2.3:53681169-53681188 None:intergenic 45.0%
TCCTACCTCGTCGACAAATT+CGG - chr2.3:53680300-53680319 MS.gene58658:intron 45.0%
TGAATCTGACATTGTCCGCA+TGG - chr2.3:53680533-53680552 MS.gene58658:intron 45.0%
!! GCTGCTTTGAAAGCCAAGAA+AGG - chr2.3:53680975-53680994 MS.gene58658:CDS 45.0%
!! GTCAGATTCAGAAGCACCTT+TGG + chr2.3:53680523-53680542 None:intergenic 45.0%
AGGGTGTGAAGTGGTAGTAG+TGG + chr2.3:53680271-53680290 None:intergenic 50.0%
TCCGAATTTGTCGACGAGGT+AGG + chr2.3:53680304-53680323 None:intergenic 50.0%
! CTCTTTGGAAGCTTTGCGTG+CGG + chr2.3:53680340-53680359 None:intergenic 50.0%
!! TCAATTGGGTTGGGATTCCC+TGG - chr2.3:53681148-53681167 MS.gene58658:CDS 50.0%
ACGAGGTAGGACACAGCGAA+AGG + chr2.3:53680291-53680310 None:intergenic 55.0%
CGAGGTAGGACACAGCGAAA+GGG + chr2.3:53680290-53680309 None:intergenic 55.0%
GAAGCTTTGCGTGCGGATTC+AGG + chr2.3:53680333-53680352 None:intergenic 55.0%
TAATCGTCAGCGCCTTACGG+TGG - chr2.3:53680797-53680816 MS.gene58658:CDS 55.0%
! CACAGCGAAAGGGTGTGAAG+TGG + chr2.3:53680280-53680299 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 53680198 53681448 53680198 ID=MS.gene58658
chr2.3 mRNA 53680198 53681448 53680198 ID=MS.gene58658.t1;Parent=MS.gene58658
chr2.3 exon 53680565 53681448 53680565 ID=MS.gene58658.t1.exon1;Parent=MS.gene58658.t1
chr2.3 CDS 53680565 53681448 53680565 ID=cds.MS.gene58658.t1;Parent=MS.gene58658.t1
chr2.3 exon 53680198 53680306 53680198 ID=MS.gene58658.t1.exon2;Parent=MS.gene58658.t1
chr2.3 CDS 53680198 53680306 53680198 ID=cds.MS.gene58658.t1;Parent=MS.gene58658.t1
Gene Sequence

>MS.gene58658

ATGTTGAAAGCATTTATCTGTCGTAATGCTTTTGGCATATCAAATTACTCACAAACACTCAGATTCCTTACCACTACTACCACTTCACACCCTTTCGCTGTGTCCTACCTCGTCGACAAATTCGGATACTCACCTGAATCCGCACGCAAAGCTTCCAAAGAGCTTTATTTCAAAACCTCACAAAAACCCGAATCAGTTATCAACTTCTTCAAAAATCATGGTTTCAATGACTCAGACATACGCAATATCATCAAAAGGGTACCAGGGATTCTTTCCTGCAATACCCACAAAACGATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTGAATCTGACATTGTCCGCATGGTTAAAGGTAACCCTCAGTTCTTGCAATTAAGCTTGAAGACTCATGAAATTAAGTTTCAATATTTTCTATCCAAAGGTGCTTCTTCTTCTGATATTGTTTCATTGTTAACTGCAAACCCTGGAATTTTACACATTAGCTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTAAGAAGGTTCTTTAAAACTGACAAGGATACCATTGTTTGCTTAATTCGACATTCAACTACATTTACTTTTAATCGTCAGCGCCTTACGGTGGCTAATATCAGTTTGATGACTGATTTTGGAGTTTCTGATTCTGTCATCGCTAATTTGCTTCACTCAAGGCCATCTATATTTGGTTCAACTGATTTGATTAAATCATTGGAGGAAGTAAAGAGTTTAGGGTTTGATCCGTCATTGACTACTTTTGGGGCTGCTTTGAAAGCCAAGAAAGGTATTAGTAAAAAACTTTGGGATGAGAAATTTGATGTCTTTAAGAAATGGGGTTGGTCCGATGAAACCGTTATTCGAGTATTTAGGTATCAGCCTAATTTGATGTTGACTTCAATTAATAAGATTAATTTGGTGATGAGATTTTGGGTCAATCAATTGGGTTGGGATTCCCTGGCACTTACTAAATGTGCACATATGTTTAGTTATAGTTTACATAAAAGGGTCATTCCAAGGGCCTCAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGGAAAAATAATGCAAGCTTAGTCACACCATTTACTTATTCTGAGGATATGTTCTTGAACAAGTTTGTTTTCAGCTTTAAGGAGGAGTCGGATTATCTGTTGAAGCTATATGAGGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGGGATGTCATCCACCAAATGTATAAGATTTTGA
Protein sequence

>MS.gene58658.t1

MLKAFICRNAFGISNYSQTLRFLTTTTTSHPFAVSYLVDKFGYSPESARKASKELYFKTSQKPESVINFFKNHGFNDSDIRNIIKRVPGILSCNTHKTILPKFQFLLSKGASESDIVRMVKGNPQFLQLSLKTHEIKFQYFLSKGASSSDIVSLLTANPGILHISLEKRIIPLFELLRRFFKTDKDTIVCLIRHSTTFTFNRQRLTVANISLMTDFGVSDSVIANLLHSRPSIFGSTDLIKSLEEVKSLGFDPSLTTFGAALKAKKGISKKLWDEKFDVFKKWGWSDETVIRVFSFKEESDYLLKLYEEKMKLANTTENNGMSSTKCIRF