AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58660.t1 MTR_2g437240 72.967 418 107 3 1 414 1 416 0.0 605
MS.gene58660.t1 MTR_2g438020 77.003 387 86 2 27 413 70 453 0.0 592
MS.gene58660.t1 MTR_2g437200 75.500 400 92 2 1 396 1 398 0.0 589
MS.gene58660.t1 MTR_2g437960 66.408 387 95 1 28 414 53 404 0.0 509
MS.gene58660.t1 MTR_2g437960 66.580 386 94 1 28 413 53 403 1.89e-180 509
MS.gene58660.t1 MTR_2g437170 63.366 404 103 4 21 414 42 410 1.32e-174 494
MS.gene58660.t1 MTR_2g437120 66.754 382 91 2 27 408 50 395 4.45e-173 490
MS.gene58660.t1 MTR_2g437150 62.907 399 105 3 21 411 42 405 3.76e-172 488
MS.gene58660.t1 MTR_2g076320 64.103 390 105 1 27 416 49 403 2.12e-171 485
MS.gene58660.t1 MTR_2g437990 64.858 387 100 2 27 413 49 399 5.70e-171 485
MS.gene58660.t1 MTR_2g438010 61.856 388 113 1 27 414 69 421 8.09e-169 479
MS.gene58660.t1 MTR_2g437160 61.266 395 111 3 21 409 50 408 2.24e-165 471
MS.gene58660.t1 MTR_2g437180 60.880 409 115 4 6 411 29 395 2.34e-165 470
MS.gene58660.t1 MTR_2g437940 59.854 411 100 3 6 416 29 374 7.74e-163 462
MS.gene58660.t1 MTR_2g437100 62.634 372 102 2 27 396 59 395 4.27e-157 449
MS.gene58660.t1 MTR_2g437130 55.867 392 131 4 22 412 37 387 9.97e-140 404
MS.gene58660.t1 MTR_2g437260 47.932 411 164 7 1 401 1 371 4.92e-124 365
MS.gene58660.t1 MTR_2g437080 47.949 390 163 3 19 406 29 380 3.79e-122 359
MS.gene58660.t1 MTR_2g437060 49.735 378 152 2 27 404 54 393 2.55e-121 358
MS.gene58660.t1 MTR_2g437020 49.614 389 153 5 20 404 29 378 2.85e-119 352
MS.gene58660.t1 MTR_2g437030 47.959 392 164 3 19 408 115 468 7.71e-118 352
MS.gene58660.t1 MTR_2g436400 45.250 400 172 5 10 400 20 381 1.24e-112 335
MS.gene58660.t1 MTR_2g436440 45.906 403 176 4 9 408 25 388 2.62e-111 332
MS.gene58660.t1 MTR_2g437040 46.174 379 166 2 30 408 42 382 4.19e-111 331
MS.gene58660.t1 MTR_4g119550 44.764 382 170 4 24 404 47 388 1.50e-107 323
MS.gene58660.t1 MTR_2g437160 68.075 213 65 1 200 409 105 317 2.76e-97 294
MS.gene58660.t1 MTR_2g437160 31.707 205 84 3 21 172 50 251 1.45e-22 97.8
MS.gene58660.t1 MTR_2g436460 46.070 369 158 5 30 397 49 377 2.24e-96 293
MS.gene58660.t1 MTR_4g120380 41.530 366 174 3 28 393 44 369 6.09e-86 266
MS.gene58660.t1 MTR_4g119580 40.659 364 176 3 30 393 46 369 4.09e-84 262
MS.gene58660.t1 MTR_4g120380 41.787 347 162 3 28 374 44 350 6.83e-80 253
MS.gene58660.t1 MTR_2g019810 41.129 372 175 7 27 396 30 359 3.88e-77 244
MS.gene58660.t1 MTR_2g019840 38.727 377 185 8 28 401 43 376 3.02e-72 232
MS.gene58660.t1 MTR_2g437090 54.386 171 78 0 239 409 1 171 3.77e-61 196
MS.gene58660.t1 MTR_4g119570 37.200 250 121 2 143 392 14 227 2.88e-46 159
MS.gene58660.t1 MTR_3g450500 27.989 368 223 5 30 393 40 369 1.01e-40 149
MS.gene58660.t1 MTR_4g007490 27.717 368 223 6 30 393 39 367 1.38e-36 137
MS.gene58660.t1 MTR_2g436370 36.279 215 90 4 10 215 19 195 1.18e-32 122
MS.gene58660.t1 MTR_4g049400 43.750 128 61 2 21 137 37 164 5.47e-28 110
MS.gene58660.t1 MTR_8g081000 25.519 337 212 3 59 393 85 384 3.77e-27 112
MS.gene58660.t1 MTR_8g081000 25.519 337 212 3 59 393 85 384 4.16e-27 112
MS.gene58660.t1 MTR_8g081000 25.519 337 212 3 59 393 85 384 5.00e-27 112
MS.gene58660.t1 MTR_8g080990 27.410 332 202 4 61 390 74 368 1.09e-25 107
MS.gene58660.t1 MTR_3g085240 57.778 90 21 1 298 387 176 248 2.39e-24 101
MS.gene58660.t1 MTR_3g085240 36.667 210 86 8 1 172 21 221 1.19e-21 94.0
MS.gene58660.t1 MTR_2g436380 56.667 60 26 0 239 298 1 60 1.16e-16 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58660.t1 AT5G07900 29.973 367 217 4 30 393 70 399 4.72e-50 174
MS.gene58660.t1 AT1G21150 30.790 367 210 5 30 391 57 384 7.72e-43 155
MS.gene58660.t1 AT1G21150 30.541 370 213 5 27 391 18 348 1.41e-42 153
MS.gene58660.t1 AT1G21150 30.790 367 210 5 30 391 121 448 1.92e-42 155
MS.gene58660.t1 AT1G61990 26.389 432 226 8 7 393 24 408 1.50e-31 124
MS.gene58660.t1 AT1G61990 26.389 432 226 8 7 393 24 408 1.50e-31 124
MS.gene58660.t1 AT1G61980 26.992 389 203 7 30 379 53 399 4.00e-29 118
MS.gene58660.t1 AT1G61980 26.992 389 203 7 30 379 53 399 4.00e-29 118
MS.gene58660.t1 AT1G61980 26.992 389 203 7 30 379 53 399 4.00e-29 118
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT1G61970 26.076 395 199 10 30 379 53 399 4.69e-27 112
MS.gene58660.t1 AT5G64950 26.705 352 240 7 57 393 27 375 4.19e-26 109
MS.gene58660.t1 AT1G61960 25.412 425 272 12 9 393 32 451 5.59e-23 100
MS.gene58660.t1 AT1G62120 25.613 367 197 8 27 356 54 381 3.59e-19 89.4
MS.gene58660.t1 AT1G62085 28.125 288 152 5 30 308 56 297 1.05e-18 88.2
MS.gene58660.t1 AT1G62085 28.125 288 152 5 30 308 56 297 1.05e-18 88.2
MS.gene58660.t1 AT5G23930 24.935 385 245 10 30 379 56 431 4.87e-18 86.3
MS.gene58660.t1 AT1G62110 26.837 313 182 7 5 309 22 295 1.94e-17 84.3
MS.gene58660.t1 AT1G62150 25.694 288 159 5 30 308 59 300 3.90e-17 83.6
MS.gene58660.t1 AT1G62010 24.576 354 188 8 64 379 71 383 4.03e-17 83.2
MS.gene58660.t1 AT3G46950 24.759 311 184 8 6 311 26 291 3.36e-16 80.5
MS.gene58660.t1 AT1G56380 25.199 377 230 17 30 379 17 368 2.39e-13 71.6
MS.gene58660.t1 AT1G56380 25.199 377 230 17 30 379 28 379 2.73e-13 71.6
MS.gene58660.t1 AT2G44020 23.779 307 207 8 66 357 147 441 1.34e-12 69.7

Find 63 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAACTAGGCCATCTATATT+TGG 0.156515 2.3:-53672958 MS.gene58660:CDS
GATCCTTCATTGGCCACTTT+TGG 0.161610 2.3:-53672886 MS.gene58660:CDS
CTGAGCGTTGTTGAAGTATT+TGG 0.204791 2.3:+53673600 None:intergenic
TAGAGGAAGTTAAGGGTTTA+GGG 0.232380 2.3:-53672912 MS.gene58660:CDS
TTTCTTATAGTTTACATAAA+AGG 0.235192 2.3:-53672642 MS.gene58660:CDS
TTAGAGGAAGTTAAGGGTTT+AGG 0.237816 2.3:-53672913 MS.gene58660:CDS
GCAAACAATGGTATCCTTAC+TGG 0.255560 2.3:+53673092 None:intergenic
TTTCTCTGTCGCAATGCTTT+TGG 0.260075 2.3:-53673627 MS.gene58660:CDS
AAGAATTTGACAACTGAATC+TGG 0.293509 2.3:+53673453 None:intergenic
AAGCTGTTATTCGAGTATTT+AGG 0.318007 2.3:-53672777 MS.gene58660:CDS
AAGCTAATGTGTAAAATTGT+AGG 0.327898 2.3:+53673165 None:intergenic
TGAATCTGACATTGTTCATA+TGG 0.329984 2.3:-53673304 MS.gene58660:CDS
GACATTGTTCATATGGTTAG+AGG 0.340870 2.3:-53673297 MS.gene58660:CDS
TAGAGGTAACCCTAAGTTCT+TGG 0.343348 2.3:-53673280 MS.gene58660:CDS
AAGCTTAATTCCAAGAACTT+AGG 0.347303 2.3:+53673270 None:intergenic
TCCTACCTCGTCGACAAATT+CGG 0.354152 2.3:-53673543 MS.gene58660:CDS
GTCAGATTCAGAAGCACCTT+TGG 0.364937 2.3:+53673317 None:intergenic
AACTTCTCAGAATAAGTAAA+TGG 0.371916 2.3:+53672544 None:intergenic
AGATTTGATTAAGTCATTAG+AGG 0.374392 2.3:-53672929 MS.gene58660:CDS
AGATCAAAATCTTATACATT+TGG 0.374741 2.3:+53672408 None:intergenic
TAAGTCATTAGAGGAAGTTA+AGG 0.381295 2.3:-53672920 MS.gene58660:CDS
GGTTGACGACTTTAAGAAAT+GGG 0.386387 2.3:-53672812 MS.gene58660:CDS
AGCTTAATTCCAAGAACTTA+GGG 0.393617 2.3:+53673271 None:intergenic
ATCAGATGAAGAAGCACCTT+TGG 0.404344 2.3:+53673212 None:intergenic
TTGGGTTAATCAAATGGGTT+GGG 0.412996 2.3:-53672698 MS.gene58660:CDS
TCAAATGGGTTGGGATTCCT+TGG 0.413582 2.3:-53672689 MS.gene58660:CDS
TTTAGGGTTTGATCCTTCAT+TGG 0.422453 2.3:-53672896 MS.gene58660:CDS
AAGTCATTAGAGGAAGTTAA+GGG 0.427528 2.3:-53672919 MS.gene58660:CDS
AGGTTGACGACTTTAAGAAA+TGG 0.446927 2.3:-53672813 MS.gene58660:CDS
TCAAAATCTTATACATTTGG+TGG 0.453156 2.3:+53672411 None:intergenic
TTGCTAGATTGCTTCAAACT+AGG 0.457873 2.3:-53672972 MS.gene58660:CDS
CAAAAGAAATTGCAAAACTA+AGG 0.463398 2.3:+53672606 None:intergenic
TTTGGGTTAATCAAATGGGT+TGG 0.473047 2.3:-53672699 MS.gene58660:CDS
CAAATACAACGGAGAACAAT+GGG 0.473276 2.3:-53672441 MS.gene58660:CDS
ACCCCAAAAGTGGCCAATGA+AGG 0.496707 2.3:+53672883 None:intergenic
AATTGCAAAACTAAGGCCCT+TGG 0.498501 2.3:+53672613 None:intergenic
ATGGAAACCAACAAAATACT+AGG 0.512435 2.3:+53672748 None:intergenic
AAACTATAAGAAAACATAAG+TGG 0.515137 2.3:+53672652 None:intergenic
AATTGATATTAGCCACCATA+AGG 0.515847 2.3:+53673028 None:intergenic
TACATAAAAGGATCATTCCA+AGG 0.520767 2.3:-53672630 MS.gene58660:CDS
AATCTTATACATTTGGTGGA+TGG 0.528179 2.3:+53672415 None:intergenic
AAGGGTGTGAAGTGATGGTA+AGG 0.538274 2.3:+53673574 None:intergenic
TAGGTTCTTCAAATCCAGTA+AGG 0.543751 2.3:-53673106 MS.gene58660:CDS
AGCGAAAGGGTGTGAAGTGA+TGG 0.551250 2.3:+53673569 None:intergenic
TACCAAAGCAACCCCAAAAG+TGG 0.559263 2.3:+53672873 None:intergenic
TTTCAGCTTTAAGGAGGAGT+CGG 0.563069 2.3:-53672503 MS.gene58660:CDS
GCAAATACAACGGAGAACAA+TGG 0.572984 2.3:-53672442 MS.gene58660:CDS
TCCGAATTTGTCGACGAGGT+AGG 0.584234 2.3:+53673542 None:intergenic
CGAGGTAGGACACAGCGAAA+GGG 0.593390 2.3:+53673556 None:intergenic
ACGACTTTAAGAAATGGGGT+TGG 0.600597 2.3:-53672807 MS.gene58660:CDS
TAATTGCCATCGCCTTATGG+TGG 0.603597 2.3:-53673040 MS.gene58660:CDS
ACGAGGTAGGACACAGCGAA+AGG 0.604629 2.3:+53673555 None:intergenic
ACATAAAAGGATCATTCCAA+GGG 0.612722 2.3:-53672629 MS.gene58660:CDS
GTTGACGACTTTAAGAAATG+GGG 0.629997 2.3:-53672811 MS.gene58660:CDS
TTCACTGACTCAGACATGAA+AGG 0.637892 2.3:-53673417 MS.gene58660:CDS
TGGACATTTAGTAAGTGCCA+AGG 0.640725 2.3:+53672672 None:intergenic
AGTTTGAAGCAATCTAGCAA+TGG 0.648223 2.3:+53672975 None:intergenic
ATGTCGAATTAAGCAAACAA+TGG 0.648992 2.3:+53673080 None:intergenic
TATTAGCCACCATAAGGCGA+TGG 0.649019 2.3:+53673034 None:intergenic
AATGAAACTTGCAAATACAA+CGG 0.655557 2.3:-53672452 MS.gene58660:CDS
GTATTGCAGGAAAGAAGCCA+TGG 0.665041 2.3:+53673378 None:intergenic
GCATCATCAAAAGAGAACCA+TGG 0.686492 2.3:-53673395 MS.gene58660:CDS
AGAATCCGAATTTGTCGACG+AGG 0.706331 2.3:+53673538 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAACAATTCAAAAAGA+GGG + chr2.3:53672926-53672945 None:intergenic 15.0%
!! TTTCTTATAGTTTACATAAA+AGG - chr2.3:53673408-53673427 MS.gene58660:CDS 15.0%
!! AAACTATAAGAAAACATAAG+TGG + chr2.3:53673401-53673420 None:intergenic 20.0%
!! AATGTTTGAGTAAAAAACTT+TGG - chr2.3:53673207-53673226 MS.gene58660:CDS 20.0%
!! AGAGTAAAAATTGAAACTTT+GGG + chr2.3:53672713-53672732 None:intergenic 20.0%
!! ATGTTTGAGTAAAAAACTTT+GGG - chr2.3:53673208-53673227 MS.gene58660:CDS 20.0%
!! CTATTTAACAATTCAAAAAG+AGG + chr2.3:53672927-53672946 None:intergenic 20.0%
!!! CTCTTTTTGAATTGTTAAAT+AGG - chr2.3:53672925-53672944 MS.gene58660:CDS 20.0%
!!! TTTGAATTTTTTCTATCCAA+AGG - chr2.3:53672822-53672841 MS.gene58660:CDS 20.0%
!!! TTTTTTACTCAAACATTTCT+TGG + chr2.3:53673204-53673223 None:intergenic 20.0%
! AACTTCTCAGAATAAGTAAA+TGG + chr2.3:53673509-53673528 None:intergenic 25.0%
! AAGCTAATGTGTAAAATTGT+AGG + chr2.3:53672888-53672907 None:intergenic 25.0%
! AATGAAACTTGCAAATACAA+CGG - chr2.3:53673598-53673617 MS.gene58660:CDS 25.0%
! AGATTTGATTAAGTCATTAG+AGG - chr2.3:53673121-53673140 MS.gene58660:CDS 25.0%
! CAAAAGAAATTGCAAAACTA+AGG + chr2.3:53673447-53673466 None:intergenic 25.0%
! GAGAGTAAAAATTGAAACTT+TGG + chr2.3:53672714-53672733 None:intergenic 25.0%
! GTCAAATTCTTCAAAAATCA+CGG - chr2.3:53672609-53672628 MS.gene58660:CDS 25.0%
! TCAAAATCTTATACATTTGG+TGG + chr2.3:53673642-53673661 None:intergenic 25.0%
!! CAACAGATTAATCTTTTCAA+TGG + chr2.3:53673324-53673343 None:intergenic 25.0%
!! TACAATTTTACACATTAGCT+TGG - chr2.3:53672887-53672906 MS.gene58660:CDS 25.0%
!! TCTTTTGAACCAAATATAGA+TGG + chr2.3:53673104-53673123 None:intergenic 25.0%
!! TTGCAATTTCTTTTGATGAA+AGG - chr2.3:53673452-53673471 MS.gene58660:CDS 25.0%
!! TTTCAATTTTTACTCTCCAA+AGG - chr2.3:53672717-53672736 MS.gene58660:CDS 25.0%
!!! AGTTTTTGGGTTAATCAAAT+GGG - chr2.3:53673347-53673366 MS.gene58660:CDS 25.0%
!!! TAATCTGTTGATGAGTTTTT+GGG - chr2.3:53673334-53673353 MS.gene58660:CDS 25.0%
!!! TTAATCTGTTGATGAGTTTT+TGG - chr2.3:53673333-53673352 MS.gene58660:CDS 25.0%
AAGAATTTGACAACTGAATC+TGG + chr2.3:53672600-53672619 None:intergenic 30.0%
AAGCTGTTATTCGAGTATTT+AGG - chr2.3:53673273-53673292 MS.gene58660:CDS 30.0%
AAGCTTAATTCCAAGAACTT+AGG + chr2.3:53672783-53672802 None:intergenic 30.0%
AAGTCATTAGAGGAAGTTAA+GGG - chr2.3:53673131-53673150 MS.gene58660:CDS 30.0%
AATTGATATTAGCCACCATA+AGG + chr2.3:53673025-53673044 None:intergenic 30.0%
ACATAAAAGGATCATTCCAA+GGG - chr2.3:53673421-53673440 MS.gene58660:CDS 30.0%
AGCTTAATTCCAAGAACTTA+GGG + chr2.3:53672782-53672801 None:intergenic 30.0%
ATGGAAACCAACAAAATACT+AGG + chr2.3:53673305-53673324 None:intergenic 30.0%
ATGTCGAATTAAGCAAACAA+TGG + chr2.3:53672973-53672992 None:intergenic 30.0%
TAAGTCATTAGAGGAAGTTA+AGG - chr2.3:53673130-53673149 MS.gene58660:CDS 30.0%
TACATAAAAGGATCATTCCA+AGG - chr2.3:53673420-53673439 MS.gene58660:CDS 30.0%
TGAATCTGACATTGTTCATA+TGG - chr2.3:53672746-53672765 MS.gene58660:CDS 30.0%
! AATCTTATACATTTGGTGGA+TGG + chr2.3:53673638-53673657 None:intergenic 30.0%
!! CAAGTTTGTTTTCAGCTTTA+AGG - chr2.3:53673538-53673557 MS.gene58660:CDS 30.0%
!! TAAAAAACTTTGGGATGAGA+AGG - chr2.3:53673217-53673236 MS.gene58660:CDS 30.0%
!!! GAGTTTTTGGGTTAATCAAA+TGG - chr2.3:53673346-53673365 MS.gene58660:CDS 30.0%
!!! TTTGTGAGGTTTTGAAAAGA+AGG + chr2.3:53672575-53672594 None:intergenic 30.0%
CAAACTAGGCCATCTATATT+TGG - chr2.3:53673092-53673111 MS.gene58660:CDS 35.0%
CAAATACAACGGAGAACAAT+GGG - chr2.3:53673609-53673628 MS.gene58660:CDS 35.0%
GACATTGTTCATATGGTTAG+AGG - chr2.3:53672753-53672772 MS.gene58660:CDS 35.0%
TAGAGGAAGTTAAGGGTTTA+GGG - chr2.3:53673138-53673157 MS.gene58660:CDS 35.0%
TAGGTTCTTCAAATCCAGTA+AGG - chr2.3:53672944-53672963 MS.gene58660:CDS 35.0%
TTAGAGGAAGTTAAGGGTTT+AGG - chr2.3:53673137-53673156 MS.gene58660:CDS 35.0%
TTGCTAGATTGCTTCAAACT+AGG - chr2.3:53673078-53673097 MS.gene58660:CDS 35.0%
! AGGTTGACGACTTTAAGAAA+TGG - chr2.3:53673237-53673256 MS.gene58660:CDS 35.0%
! AGTTTGAAGCAATCTAGCAA+TGG + chr2.3:53673078-53673097 None:intergenic 35.0%
! GGTTGACGACTTTAAGAAAT+GGG - chr2.3:53673238-53673257 MS.gene58660:CDS 35.0%
! GTTGACGACTTTAAGAAATG+GGG - chr2.3:53673239-53673258 MS.gene58660:CDS 35.0%
! TTGGGTTAATCAAATGGGTT+GGG - chr2.3:53673352-53673371 MS.gene58660:CDS 35.0%
! TTTAGGGTTTGATCCTTCAT+TGG - chr2.3:53673154-53673173 MS.gene58660:CDS 35.0%
! TTTGGGTTAATCAAATGGGT+TGG - chr2.3:53673351-53673370 MS.gene58660:CDS 35.0%
!! AATTGTTTTGTGGGTATTGC+AGG + chr2.3:53672688-53672707 None:intergenic 35.0%
!! ACTTTGGGAGAATTGTTTTG+TGG + chr2.3:53672698-53672717 None:intergenic 35.0%
!! CTTTGGGAGAATTGTTTTGT+GGG + chr2.3:53672697-53672716 None:intergenic 35.0%
!! TTTTAATTGCCATCGCCTTA+TGG - chr2.3:53673007-53673026 MS.gene58660:CDS 35.0%
!!! AACTGAATCTGGTTTTTGTG+AGG + chr2.3:53672589-53672608 None:intergenic 35.0%
!!! GTTTGTTTTCAGCTTTAAGG+AGG - chr2.3:53673541-53673560 MS.gene58660:CDS 35.0%
AATTGCAAAACTAAGGCCCT+TGG + chr2.3:53673440-53673459 None:intergenic 40.0%
GCAAACAATGGTATCCTTAC+TGG + chr2.3:53672961-53672980 None:intergenic 40.0%
GCAAATACAACGGAGAACAA+TGG - chr2.3:53673608-53673627 MS.gene58660:CDS 40.0%
GCATCATCAAAAGAGAACCA+TGG - chr2.3:53672655-53672674 MS.gene58660:CDS 40.0%
TAGAGGTAACCCTAAGTTCT+TGG - chr2.3:53672770-53672789 MS.gene58660:CDS 40.0%
TGGACATTTAGTAAGTGCCA+AGG + chr2.3:53673381-53673400 None:intergenic 40.0%
! ACGACTTTAAGAAATGGGGT+TGG - chr2.3:53673243-53673262 MS.gene58660:CDS 40.0%
! CTGAGCGTTGTTGAAGTATT+TGG + chr2.3:53672453-53672472 None:intergenic 40.0%
! GTGTCGTCCTAGTATTTTGT+TGG - chr2.3:53673295-53673314 MS.gene58660:CDS 40.0%
! TTTCTCTGTCGCAATGCTTT+TGG - chr2.3:53672423-53672442 MS.gene58660:CDS 40.0%
!! ATCAGATGAAGAAGCACCTT+TGG + chr2.3:53672841-53672860 None:intergenic 40.0%
!! TTCACTGACTCAGACATGAA+AGG - chr2.3:53672633-53672652 MS.gene58660:CDS 40.0%
!! TTTCAGCTTTAAGGAGGAGT+CGG - chr2.3:53673547-53673566 MS.gene58660:CDS 40.0%
!!! ATCCTTCATTGGCCACTTTT+GGG - chr2.3:53673165-53673184 MS.gene58660:CDS 40.0%
AGAATCCGAATTTGTCGACG+AGG + chr2.3:53672515-53672534 None:intergenic 45.0%
GTATTGCAGGAAAGAAGCCA+TGG + chr2.3:53672675-53672694 None:intergenic 45.0%
TAATTGCCATCGCCTTATGG+TGG - chr2.3:53673010-53673029 MS.gene58660:CDS 45.0%
TACCAAAGCAACCCCAAAAG+TGG + chr2.3:53673180-53673199 None:intergenic 45.0%
TATTAGCCACCATAAGGCGA+TGG + chr2.3:53673019-53673038 None:intergenic 45.0%
TCCTACCTCGTCGACAAATT+CGG - chr2.3:53672507-53672526 MS.gene58660:CDS 45.0%
! AAGGGTGTGAAGTGATGGTA+AGG + chr2.3:53672479-53672498 None:intergenic 45.0%
!! GATCCTTCATTGGCCACTTT+TGG - chr2.3:53673164-53673183 MS.gene58660:CDS 45.0%
!! GTCAGATTCAGAAGCACCTT+TGG + chr2.3:53672736-53672755 None:intergenic 45.0%
!! TCAAATGGGTTGGGATTCCT+TGG - chr2.3:53673361-53673380 MS.gene58660:CDS 45.0%
!!! TCCTTCATTGGCCACTTTTG+GGG - chr2.3:53673166-53673185 MS.gene58660:CDS 45.0%
ACCCCAAAAGTGGCCAATGA+AGG + chr2.3:53673170-53673189 None:intergenic 50.0%
TCCGAATTTGTCGACGAGGT+AGG + chr2.3:53672511-53672530 None:intergenic 50.0%
! AGCGAAAGGGTGTGAAGTGA+TGG + chr2.3:53672484-53672503 None:intergenic 50.0%
ACGAGGTAGGACACAGCGAA+AGG + chr2.3:53672498-53672517 None:intergenic 55.0%
CGAGGTAGGACACAGCGAAA+GGG + chr2.3:53672497-53672516 None:intergenic 55.0%
!!! GGCCACTTTTGGGGTTGCTT+TGG - chr2.3:53673175-53673194 MS.gene58660:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 53672411 53673661 53672411 ID=MS.gene58660
chr2.3 mRNA 53672411 53673661 53672411 ID=MS.gene58660.t1;Parent=MS.gene58660
chr2.3 exon 53672411 53673661 53672411 ID=MS.gene58660.t1.exon1;Parent=MS.gene58660.t1
chr2.3 CDS 53672411 53673661 53672411 ID=cds.MS.gene58660.t1;Parent=MS.gene58660.t1
Gene Sequence

>MS.gene58660

ATGTTGAAAGCATTTCTCTGTCGCAATGCTTTTGGCATACCAAATACTTCAACAACGCTCAGATTCCTTACCATCACTTCACACCCTTTCGCTGTGTCCTACCTCGTCGACAAATTCGGATTCTCACATGAATTTGCACTCAAAGCTTCAGAATCAGAACACCTTCTTTTCAAAACCTCACAAAAACCAGATTCAGTTGTCAAATTCTTCAAAAATCACGGTTTCACTGACTCAGACATGAAAGGCATCATCAAAAGAGAACCATGGCTTCTTTCCTGCAATACCCACAAAACAATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTGAATCTGACATTGTTCATATGGTTAGAGGTAACCCTAAGTTCTTGGAATTAAGCTTGAAGAATCATCAAATTAAGTTTGAATTTTTTCTATCCAAAGGTGCTTCTTCATCTGATATTGTTTCATTGTTAACTAAAAATCCTACAATTTTACACATTAGCTTGGAGAAACAAATAATCCCTCTTTTTGAATTGTTAAATAGGTTCTTCAAATCCAGTAAGGATACCATTGTTTGCTTAATTCGACATTCAACTACATTTGCTTTTAATTGCCATCGCCTTATGGTGGCTAATATCAATTTGATGATTGATTTTGAAGTTTCTGATTCTACCATTGCTAGATTGCTTCAAACTAGGCCATCTATATTTGGTTCAAAAGATTTGATTAAGTCATTAGAGGAAGTTAAGGGTTTAGGGTTTGATCCTTCATTGGCCACTTTTGGGGTTGCTTTGGTAGCCAAGAAATGTTTGAGTAAAAAACTTTGGGATGAGAAGGTTGACGACTTTAAGAAATGGGGTTGGTCTGATGAAGCTGTTATTCGAGTATTTAGGTGTCGTCCTAGTATTTTGTTGGTTTCCATTGAAAAGATTAATCTGTTGATGAGTTTTTGGGTTAATCAAATGGGTTGGGATTCCTTGGCACTTACTAAATGTCCACTTATGTTTTCTTATAGTTTACATAAAAGGATCATTCCAAGGGCCTTAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCAAGCTTAGTTATACCATTTACTTATTCTGAGAAGTTGTTCTTGAACAAGTTTGTTTTCAGCTTTAAGGAGGAGTCGGATTATCTGTTGAAGCTATATGACGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGGGATGCCATCCACCAAATGTATAAGATTTTGA
Protein sequence

>MS.gene58660.t1

MLKAFLCRNAFGIPNTSTTLRFLTITSHPFAVSYLVDKFGFSHEFALKASESEHLLFKTSQKPDSVVKFFKNHGFTDSDMKGIIKREPWLLSCNTHKTILPKFQFLLSKGASESDIVHMVRGNPKFLELSLKNHQIKFEFFLSKGASSSDIVSLLTKNPTILHISLEKQIIPLFELLNRFFKSSKDTIVCLIRHSTTFAFNCHRLMVANINLMIDFEVSDSTIARLLQTRPSIFGSKDLIKSLEEVKGLGFDPSLATFGVALVAKKCLSKKLWDEKVDDFKKWGWSDEAVIRVFRCRPSILLVSIEKINLLMSFWVNQMGWDSLALTKCPLMFSYSLHKRIIPRALVLQFLLMKGLRKKNASLVIPFTYSEKLFLNKFVFSFKEESDYLLKLYDEKMKLANTTENNGMPSTKCIRF