AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58827

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58827.t1 MTR_7g088890 97.650 766 13 2 1 762 1 765 0.0 1533
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58827.t1 AT2G40950 48.810 756 288 23 28 753 26 712 9.25e-172 511
MS.gene58827.t1 AT3G56660 43.519 540 227 14 227 753 137 611 5.63e-119 372
MS.gene58827.t1 AT3G10800 42.656 640 266 19 140 753 103 667 8.82e-115 362

Find 230 sgRNAs with CRISPR-Local

Find 297 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATCAATCACATGCGAAAT+TGG 0.236893 7.3:-20518066 None:intergenic
CTTGATAATCCATTCTATTA+TGG 0.239062 7.3:+20519391 MS.gene58827:CDS
TCAGGTGCTTCTGTGGTTTC+CGG 0.251766 7.3:+20518099 MS.gene58827:CDS
GGTTTAGTGGATAATGTTTC+GGG 0.254398 7.3:+20519080 MS.gene58827:CDS
CGGAAAGACTCGAGTTGTTT+TGG 0.260662 7.3:+20519287 MS.gene58827:CDS
ATCAGAGTCCTGGTGTTTCT+AGG 0.274337 7.3:+20518124 MS.gene58827:CDS
CTGGATGCGGTGGGTACATC+GGG 0.292468 7.3:-20518803 None:intergenic
ATCGCCATCTCCTACCTCTT+TGG 0.292842 7.3:-20520627 None:intergenic
TTGGAGGGAAGTGTTGTTAA+AGG 0.298541 7.3:+20518405 MS.gene58827:CDS
CCTCTATTTCTTCCAACTTC+AGG 0.303811 7.3:-20519506 None:intergenic
TGGTTTAGTGGATAATGTTT+CGG 0.306602 7.3:+20519079 MS.gene58827:CDS
GAATGGACCGGTTTCGTCTC+AGG 0.309073 7.3:+20518281 MS.gene58827:CDS
TCGAAGACTCTTCCCGTTCC+CGG 0.316652 7.3:-20518260 None:intergenic
ACCCTCCCAGATCCACTTCC+TGG 0.325977 7.3:+20520506 MS.gene58827:CDS
CCGGTTTCGTCTCAGGGATC+GGG 0.337795 7.3:+20518288 MS.gene58827:CDS
GCACCAGGTGGTGGTGGTGG+TGG 0.337944 7.3:-20518780 None:intergenic
TCAAGGTTCTCAGGTTCCTT+TGG 0.340949 7.3:+20518881 MS.gene58827:CDS
CGGCTTCCAGGAAGTGGATC+TGG 0.346167 7.3:-20520512 None:intergenic
TTTGATGTTTCTCCAGCTCC+TGG 0.346463 7.3:+20520386 MS.gene58827:CDS
TCATTCATATGACCATTTAC+AGG 0.346777 7.3:-20519161 None:intergenic
GAGAAAGTGCACAGCTTTCA+AGG 0.360135 7.3:+20518613 MS.gene58827:CDS
ATATTTGCCACTGCTGTTGC+TGG 0.364817 7.3:-20520419 None:intergenic
GGGAAGATTTGGCCTGTAAA+TGG 0.365839 7.3:+20519149 MS.gene58827:CDS
GGAGATTTGGTATTTCTGAT+AGG 0.374820 7.3:+20519219 MS.gene58827:CDS
GTGAAACTGGTCTGGCCATT+CGG 0.374866 7.3:+20519465 MS.gene58827:CDS
GGGGCACCAGGTGGTGGTGG+TGG 0.375258 7.3:-20518783 None:intergenic
GTCATGGTGGAGGGAAGATT+TGG 0.376373 7.3:+20519138 MS.gene58827:CDS
TGAAACTGGTCTGGCCATTC+GGG 0.376516 7.3:+20519466 MS.gene58827:CDS
TCGCCATCTCCTACCTCTTT+GGG 0.378134 7.3:-20520626 None:intergenic
TGTGAAAGAGGATGTGAAAT+TGG 0.378389 7.3:+20518386 MS.gene58827:CDS
TCAGAGGACGGTGGTTGTTC+CGG 0.379457 7.3:-20517847 None:intergenic
TCTTCCCCGTGTTTCTCCTT+TGG 0.379638 7.3:+20520739 MS.gene58827:CDS
AAAACACTGAAAATTCTTAC+CGG 0.387081 7.3:+20518241 MS.gene58827:CDS
GGGTTTCCCAATGGGAGATT+TGG 0.388690 7.3:+20519206 MS.gene58827:CDS
TTGCCGGGGAGGTGGTCTTT+AGG 0.391830 7.3:-20520569 None:intergenic
CATCAGTCTCCGACGGGATT+GGG 0.393503 7.3:-20518005 None:intergenic
ATTGATAATTCGCCGGAATC+AGG 0.396736 7.3:+20518081 MS.gene58827:CDS
GTTAGTGATAGATGGTTCTA+TGG 0.397227 7.3:+20519116 MS.gene58827:CDS
TACATCGGGGCACCAGGTGG+TGG 0.397975 7.3:-20518789 None:intergenic
GGATCGGGGAACGGTGGATC+GGG 0.403786 7.3:+20518303 MS.gene58827:CDS
GACCATAGATGATTTATTGC+CGG 0.403947 7.3:-20520585 None:intergenic
ACCACAGAAGCACCTGATTC+CGG 0.404825 7.3:-20518093 None:intergenic
GTTATGTTCAGACGGCTTCC+AGG 0.404925 7.3:-20520524 None:intergenic
GCTGAACAGAGGAGGGCTAT+TGG 0.405451 7.3:+20519557 MS.gene58827:CDS
CCTTGAGGTTTAACAACATA+AGG 0.407763 7.3:-20518864 None:intergenic
GTTGCTAGTATTAGTTTGTT+GGG 0.408388 7.3:+20518996 MS.gene58827:CDS
TGAAAATTCTTACCGGGAAC+GGG 0.409395 7.3:+20518248 MS.gene58827:CDS
TTGCTTCTTTGTATGTTCCT+AGG 0.410557 7.3:+20519339 MS.gene58827:CDS
AATGGTCATATGAATGAATC+TGG 0.410603 7.3:+20519167 MS.gene58827:CDS
TCATCAGTCTCCGACGGGAT+TGG 0.412434 7.3:-20518006 None:intergenic
AGTTGCTAGTATTAGTTTGT+TGG 0.413297 7.3:+20518995 MS.gene58827:CDS
CCCCGGCAATAAATCATCTA+TGG 0.413609 7.3:+20520583 MS.gene58827:CDS
TCAACATCCAAATGTGTATA+GGG 0.416871 7.3:+20519529 MS.gene58827:CDS
GTCAACATCCAAATGTGTAT+AGG 0.417075 7.3:+20519528 MS.gene58827:CDS
TCTGGAAGGAATGGGGAAGC+GGG 0.417527 7.3:+20519185 MS.gene58827:CDS
CTAGTTATGTTAGTGATAGA+TGG 0.419905 7.3:+20519108 MS.gene58827:CDS
GCAGAAACCCTATACACATT+TGG 0.422665 7.3:-20519536 None:intergenic
TAAGGCATATGTGCCATTCC+TGG 0.424550 7.3:-20518822 None:intergenic
CCTGGATGCGGTGGGTACAT+CGG 0.426624 7.3:-20518804 None:intergenic
ACCATAGATGATTTATTGCC+GGG 0.430999 7.3:-20520584 None:intergenic
ACCGGTTTCGTCTCAGGGAT+CGG 0.431238 7.3:+20518287 MS.gene58827:CDS
TTTCAACAACAAGCTCAATC+TGG 0.433911 7.3:+20518534 MS.gene58827:CDS
AAGAAGGAGAAGAGTGAAAC+TGG 0.434906 7.3:+20519452 MS.gene58827:CDS
GACGGTGGTTGTTCCGGCGG+TGG 0.439486 7.3:-20517841 None:intergenic
TGTTAAACCTCAAGGTTCTC+AGG 0.442420 7.3:+20518872 MS.gene58827:CDS
TGGTTCCGTGAAGGAATTGC+AGG 0.443208 7.3:+20519647 MS.gene58827:CDS
CTGAAAATTCTTACCGGGAA+CGG 0.446378 7.3:+20518247 MS.gene58827:CDS
ATGAATGAATCTGGAAGGAA+TGG 0.448209 7.3:+20519176 MS.gene58827:CDS
AGATGCCATGGCTTTCTCAC+TGG 0.450554 7.3:-20519414 None:intergenic
GAAGTGGATCTGGGAGGGTG+TGG 0.450659 7.3:-20520502 None:intergenic
GGAACTATTGCTCCAGGAGC+TGG 0.454713 7.3:-20520398 None:intergenic
CAGAGGAGGGCTATTGGCTC+TGG 0.455745 7.3:+20519563 MS.gene58827:CDS
GGGATCGGGGAACGGTGGAT+CGG 0.458209 7.3:+20518302 MS.gene58827:CDS
GCATTGGCAATTCCTGAAGT+TGG 0.458388 7.3:+20519494 MS.gene58827:CDS
TGAGGCTTCAACCTCGGAAT+CGG 0.460632 7.3:-20518906 None:intergenic
GGGTACATCGGGGCACCAGG+TGG 0.461707 7.3:-20518792 None:intergenic
AATGGACCGGTTTCGTCTCA+GGG 0.463557 7.3:+20518282 MS.gene58827:CDS
GAAGCCGCAGCAGTTGGCTG+AGG 0.464585 7.3:-20518924 None:intergenic
AGGATGAGAAGAGGAAAGCA+AGG 0.465492 7.3:+20518577 MS.gene58827:CDS
GTAGGAGATGGCGATGTTGA+TGG 0.465751 7.3:+20520635 MS.gene58827:CDS
CGACCTCCTACTACATTTCG+AGG 0.468018 7.3:-20518481 None:intergenic
ATCGGGGCACCAGGTGGTGG+TGG 0.469118 7.3:-20518786 None:intergenic
AGACTTCATATGGTCCTTCA+AGG 0.470842 7.3:-20519597 None:intergenic
GGTGGGTACATCGGGGCACC+AGG 0.476383 7.3:-20518795 None:intergenic
CCTTATGTTGTTAAACCTCA+AGG 0.476907 7.3:+20518864 MS.gene58827:CDS
TTCAGACGGCTTCCAGGAAG+TGG 0.479106 7.3:-20520518 None:intergenic
GAAATACCAAATCTCCCATT+GGG 0.479124 7.3:-20519212 None:intergenic
GATCCCGATCTCGATTTCCT+TGG 0.479477 7.3:+20517946 MS.gene58827:CDS
GGCCATTCGGGATTCTGCAT+TGG 0.480883 7.3:+20519478 MS.gene58827:CDS
GGGAAGCGGGGTTTCCCAAT+GGG 0.481766 7.3:+20519198 MS.gene58827:CDS
GGCTTCCAGGAAGTGGATCT+GGG 0.482830 7.3:-20520511 None:intergenic
CCGATGTACCCACCGCATCC+AGG 0.483203 7.3:+20518804 MS.gene58827:CDS
GAGCTGGAGAAACATCAAAC+TGG 0.484452 7.3:-20520382 None:intergenic
ATGATTTATTGCCGGGGAGG+TGG 0.484620 7.3:-20520577 None:intergenic
CATGGCATCTCAAGATGCTC+AGG 0.484756 7.3:+20519427 MS.gene58827:CDS
AGTTCTGAGCTTTCCGTCAC+CGG 0.486087 7.3:+20518206 MS.gene58827:CDS
TCTCACTGGCCATAATAGAA+TGG 0.486529 7.3:-20519400 None:intergenic
GCAGGTCCAATGCTAAGTTC+TGG 0.487433 7.3:+20520341 MS.gene58827:intron
TGGTTCTATGGTCATGGTGG+AGG 0.488669 7.3:+20519128 MS.gene58827:CDS
TGGTTCAACTAAAACCTTGA+AGG 0.490584 7.3:+20519583 MS.gene58827:CDS
TATGAAAGAGGGAGGAAACT+AGG 0.490874 7.3:+20519248 MS.gene58827:CDS
CTCTTCGAAGCCGCAGCAGT+TGG 0.491101 7.3:-20518930 None:intergenic
ATCTGGAAGGAATGGGGAAG+CGG 0.494855 7.3:+20519184 MS.gene58827:CDS
TGAATGAATCTGGAAGGAAT+GGG 0.497476 7.3:+20519177 MS.gene58827:CDS
CAGTTGCAGAAGACTTCATA+TGG 0.499404 7.3:-20519607 None:intergenic
TCGTCTCAGGGATCGGGGAA+CGG 0.500301 7.3:+20518294 MS.gene58827:CDS
GATAGGAATAATTATGAAAG+AGG 0.503581 7.3:+20519236 MS.gene58827:CDS
GGAGAAGAGTGAAACTGGTC+TGG 0.504348 7.3:+20519457 MS.gene58827:CDS
AGAGGAAAGCAAGGTTGATG+AGG 0.504690 7.3:+20518586 MS.gene58827:CDS
GTGGATCTGGGAGGGTGTGG+AGG 0.505432 7.3:-20520499 None:intergenic
CCTGAAGTTGGAAGAAATAG+AGG 0.507024 7.3:+20519506 MS.gene58827:CDS
AGCGATGAATTCGCCGGAGA+GGG 0.507085 7.3:+20518335 MS.gene58827:CDS
CCCGATCCCTGAGACGAAAC+CGG 0.507645 7.3:-20518288 None:intergenic
CTGAGCATCTTGAGATGCCA+TGG 0.508247 7.3:-20519426 None:intergenic
TCCAGGAAGTGGATCTGGGA+GGG 0.511493 7.3:-20520507 None:intergenic
ATTCACCTGCAATTCCTTCA+CGG 0.511676 7.3:-20519652 None:intergenic
GCATTATGTGGAGGAGCTTG+AGG 0.515226 7.3:+20518647 MS.gene58827:CDS
GTTGCTGGAACTATTGCTCC+AGG 0.515460 7.3:-20520404 None:intergenic
TCAAGATGCTCAGGGGAAGA+AGG 0.516725 7.3:+20519436 MS.gene58827:CDS
TGAAAATGAAAATGTGAAAG+AGG 0.516780 7.3:+20518374 MS.gene58827:CDS
GCACAGGGCATCCATGCATA+AGG 0.516883 7.3:-20518840 None:intergenic
CCTGCTCTTGTTGACAGTGG+TGG 0.518840 7.3:-20520465 None:intergenic
TTTGAATTTGGATTCAGTTG+CGG 0.525278 7.3:+20518149 MS.gene58827:CDS
TGGCTCCGAAGTCGCTCTCT+CGG 0.526704 7.3:+20520663 MS.gene58827:CDS
GTGCACAGCTTTCAAGGCAG+AGG 0.529811 7.3:+20518619 MS.gene58827:CDS
AGAAATACCAAATCTCCCAT+TGG 0.530021 7.3:-20519213 None:intergenic
TTTAACAACATAAGGAGCAC+AGG 0.531090 7.3:-20518856 None:intergenic
ATCGCCAAGGAAATCGAGAT+CGG 0.531775 7.3:-20517950 None:intergenic
TTCACCAGCTTATCATTCCT+AGG 0.531777 7.3:-20519356 None:intergenic
TCGCCAAGGAAATCGAGATC+GGG 0.535175 7.3:-20517949 None:intergenic
GGGGAAGCGGGGTTTCCCAA+TGG 0.536329 7.3:+20519197 MS.gene58827:CDS
AAAATCCGAGAGAGCGACTT+CGG 0.540450 7.3:-20520668 None:intergenic
ATCGAACCTCGAAATGTAGT+AGG 0.541329 7.3:+20518475 MS.gene58827:CDS
GTTGGCTGAGGCTTCAACCT+CGG 0.543196 7.3:-20518912 None:intergenic
TTGTTCCGGCGGTGGTTGAA+CGG 0.544558 7.3:-20517833 None:intergenic
TTGCCTAAAGACCACCTCCC+CGG 0.545652 7.3:+20520566 MS.gene58827:CDS
GATAAGCTGGTGAAGATCGA+TGG 0.548077 7.3:+20519365 MS.gene58827:CDS
TAGTAAGATTACGTATGTAA+TGG 0.550072 7.3:+20518710 MS.gene58827:CDS
TTGAAGAAGGAGTCAAATGA+AGG 0.550107 7.3:-20517916 None:intergenic
GCTCTTCAGTTATGTTCAGA+CGG 0.550955 7.3:-20520532 None:intergenic
TTCCTTTGGTTCCGATTCCG+AGG 0.552849 7.3:+20518895 MS.gene58827:CDS
CCACCACTGTCAACAAGAGC+AGG 0.555668 7.3:+20520465 MS.gene58827:CDS
GCCGGAATCAGGTGCTTCTG+TGG 0.555970 7.3:+20518092 MS.gene58827:CDS
GGAAGAGTCTTCGAATGGAC+CGG 0.556021 7.3:+20518269 MS.gene58827:CDS
TAGTAGGAGGTCGTTGTCGA+TGG 0.558283 7.3:+20518491 MS.gene58827:CDS
CGGTTTCGTCTCAGGGATCG+GGG 0.559544 7.3:+20518289 MS.gene58827:CDS
GGGATCTGAATCGTTGAAGA+AGG 0.560063 7.3:-20517929 None:intergenic
GTCGTTGTCGATGGAGAGGA+CGG 0.563859 7.3:+20518500 MS.gene58827:CDS
ATAGGAATAATTATGAAAGA+GGG 0.566000 7.3:+20519237 MS.gene58827:CDS
TTAAAGGCTGTGATCTGAAG+AGG 0.569436 7.3:+20518421 MS.gene58827:CDS
TAGTGGATAATGTTTCGGGT+AGG 0.576691 7.3:+20519084 MS.gene58827:CDS
TCTCAGGGATCGGGGAACGG+TGG 0.577492 7.3:+20518297 MS.gene58827:CDS
AAAGATGAAACATCCCTCTC+CGG 0.578091 7.3:-20518348 None:intergenic
CACATGCCAGAACTTAGCAT+TGG 0.578294 7.3:-20520347 None:intergenic
GGAATAATTATGAAAGAGGG+AGG 0.578317 7.3:+20519240 MS.gene58827:CDS
TTGAAGATGAGGATGAGAAG+AGG 0.578374 7.3:+20518568 MS.gene58827:CDS
ATGAAGTCTTCTGCAACTGA+TGG 0.579273 7.3:+20519611 MS.gene58827:CDS
GTTTCCGGTGATCAGAGTCC+TGG 0.579472 7.3:+20518114 MS.gene58827:CDS
GGTTCTATGGTCATGGTGGA+GGG 0.579901 7.3:+20519129 MS.gene58827:CDS
GCAGAGGAAGAAGCATTATG+TGG 0.581328 7.3:+20518635 MS.gene58827:CDS
GTCATATGAATGAATCTGGA+AGG 0.581451 7.3:+20519171 MS.gene58827:CDS
GTTCCTGCTCTTGTTGACAG+TGG 0.583791 7.3:-20520468 None:intergenic
GTACCCACCGCATCCAGGAA+TGG 0.584929 7.3:+20518809 MS.gene58827:CDS
TGTTCCTAGGAATGATAAGC+TGG 0.588162 7.3:+20519352 MS.gene58827:CDS
GCATGTGATTGATAATTCGC+CGG 0.589032 7.3:+20518074 MS.gene58827:CDS
AGATAATCATCAGTCTCCGA+CGG 0.590359 7.3:-20518012 None:intergenic
GAGTGAGAGTAAGAAGAGTG+AGG 0.592637 7.3:+20518959 MS.gene58827:CDS
GGGTTTCTGCTGAACAGAGG+AGG 0.593853 7.3:+20519549 MS.gene58827:CDS
GATAATCATCAGTCTCCGAC+GGG 0.596708 7.3:-20518011 None:intergenic
CTTGTTGATCCCAAAGAGGT+AGG 0.597487 7.3:+20520617 MS.gene58827:CDS
GATAGATGGTTCTATGGTCA+TGG 0.600127 7.3:+20519122 MS.gene58827:CDS
AAGCGATGAATTCGCCGGAG+AGG 0.602075 7.3:+20518334 MS.gene58827:CDS
AACCTCGGAATCGGAACCAA+AGG 0.604998 7.3:-20518897 None:intergenic
TGGCGATGTTGATGGCATGA+TGG 0.608428 7.3:+20520643 MS.gene58827:CDS
GAAGCCTCAGCCAACTGCTG+CGG 0.610900 7.3:+20518920 MS.gene58827:CDS
GAATGAATCTGGAAGGAATG+GGG 0.612489 7.3:+20519178 MS.gene58827:CDS
ATGCAACAGTGGTTCCGTGA+AGG 0.612772 7.3:+20519638 MS.gene58827:CDS
GAACGGGAAGAGTCTTCGAA+TGG 0.615655 7.3:+20518264 MS.gene58827:CDS
GATCCCAAAGAGGTAGGAGA+TGG 0.616124 7.3:+20520623 MS.gene58827:CDS
CTGGAAGGAATGGGGAAGCG+GGG 0.617811 7.3:+20519186 MS.gene58827:CDS
GAGGAAGAAGCATTATGTGG+AGG 0.618127 7.3:+20518638 MS.gene58827:CDS
GAGGACGGTGGTTGTTCCGG+CGG 0.619778 7.3:-20517844 None:intergenic
CTGTCACCAAAGGAGAAACA+CGG 0.620177 7.3:-20520745 None:intergenic
AACACCAGGACTCTGATCAC+CGG 0.620315 7.3:-20518118 None:intergenic
GGTTTCTGCTGAACAGAGGA+GGG 0.625544 7.3:+20519550 MS.gene58827:CDS
AAACACTGAAAATTCTTACC+GGG 0.626166 7.3:+20518242 MS.gene58827:CDS
TGTCACCAAAGGAGAAACAC+GGG 0.626669 7.3:-20520744 None:intergenic
CATGATGCATGCAACGCTGA+CGG 0.627417 7.3:+20518039 MS.gene58827:CDS
ATATGTGCCATTCCTGGATG+CGG 0.627958 7.3:-20518816 None:intergenic
TGGCTGAGAATGCTACACTT+AGG 0.630909 7.3:+20518730 MS.gene58827:CDS
TGTGCCATTCCTGGATGCGG+TGG 0.631125 7.3:-20518813 None:intergenic
GAACCTCGAAATGTAGTAGG+AGG 0.634877 7.3:+20518478 MS.gene58827:CDS
TGTGCTTGTTGATCCCAAAG+AGG 0.635244 7.3:+20520613 MS.gene58827:CDS
TGGCACATATGCCTTATGCA+TGG 0.636864 7.3:+20518829 MS.gene58827:CDS
TGCCAATGCAGAATCCCGAA+TGG 0.636929 7.3:-20519480 None:intergenic
TTCCAGGAAGTGGATCTGGG+AGG 0.637184 7.3:-20520508 None:intergenic
CAAACATAAGCTGTCACCAA+AGG 0.638493 7.3:-20520755 None:intergenic
TCTCACCGTTCAACCACCGC+CGG 0.639884 7.3:+20517828 MS.gene58827:CDS
GAAAGAGGATGTGAAATTGG+AGG 0.644180 7.3:+20518389 MS.gene58827:CDS
TATGGCCAGTGAGAAAGCCA+TGG 0.645832 7.3:+20519409 MS.gene58827:CDS
GATCTCGATTTCAACGACTA+CGG 0.647229 7.3:+20517868 MS.gene58827:CDS
ATGGCATCTCAAGATGCTCA+GGG 0.649184 7.3:+20519428 MS.gene58827:CDS
CCAAACTTAAAATCTACCAA+AGG 0.650046 7.3:-20519056 None:intergenic
GGAGGTCGTTGTCGATGGAG+AGG 0.650672 7.3:+20518496 MS.gene58827:CDS
GTCGTTGAAATCGAGATCAG+AGG 0.650830 7.3:-20517863 None:intergenic
CTCGATTTCAACGACTACGG+TGG 0.651643 7.3:+20517871 MS.gene58827:CDS
TAGATGATTTATTGCCGGGG+AGG 0.653488 7.3:-20520580 None:intergenic
ACACTTAGGCAACAATTGAG+TGG 0.653994 7.3:+20518744 MS.gene58827:CDS
GTATGAAGCGATGAATTCGC+CGG 0.654691 7.3:+20518329 MS.gene58827:CDS
GGAGTCAAATGAAGGAAGAG+CGG 0.659484 7.3:-20517908 None:intergenic
AAAGAGGATGTGAAATTGGA+GGG 0.661470 7.3:+20518390 MS.gene58827:CDS
ACGTGATTTCGAAATCGCCA+AGG 0.661829 7.3:-20517963 None:intergenic
GTGCCATTCCTGGATGCGGT+GGG 0.664412 7.3:-20518812 None:intergenic
CTGATGGTAAAATGCAACAG+TGG 0.665578 7.3:+20519627 MS.gene58827:CDS
CCATAGATGATTTATTGCCG+GGG 0.667485 7.3:-20520583 None:intergenic
AATAGTTCCAGCAACAGCAG+TGG 0.669198 7.3:+20520412 MS.gene58827:CDS
TAGGTGAAGAAATGAATGAG+CGG 0.675700 7.3:+20519267 MS.gene58827:CDS
AGATAATCTCCCAATCCCGT+CGG 0.677244 7.3:+20517996 MS.gene58827:CDS
TTAACAACATAAGGAGCACA+GGG 0.677664 7.3:-20518855 None:intergenic
TGGATGCGGTGGGTACATCG+GGG 0.678733 7.3:-20518802 None:intergenic
AAAGGAACCTGAGAACCTTG+AGG 0.689564 7.3:-20518879 None:intergenic
ACATACAAAGAAGCAAGCAG+TGG 0.691640 7.3:-20519332 None:intergenic
AAGTATGTGACTTACTCATG+TGG 0.694107 7.3:+20520716 MS.gene58827:CDS
AGATGGTTCTATGGTCATGG+TGG 0.697055 7.3:+20519125 MS.gene58827:CDS
ATAGGGTTTCTGCTGAACAG+AGG 0.697311 7.3:+20519546 MS.gene58827:CDS
CTTAGGCAACAATTGAGTGG+TGG 0.698725 7.3:+20518747 MS.gene58827:CDS
TTGAAATCGAGATCAGAGGA+CGG 0.719221 7.3:-20517859 None:intergenic
TGGCATCTCAAGATGCTCAG+GGG 0.720143 7.3:+20519429 MS.gene58827:CDS
AAATCGAGATCAGAGGACGG+TGG 0.738506 7.3:-20517856 None:intergenic
GTCACCAAAGGAGAAACACG+GGG 0.765380 7.3:-20520743 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGGAATAATTATGAAAGA+GGG + chr7.3:20519237-20519256 MS.gene58827:CDS 20.0%
!!! TTTTTCTGTATTATGCTTTT+TGG + chr7.3:20519023-20519042 MS.gene58827:CDS 20.0%
! AAAACACTGAAAATTCTTAC+CGG + chr7.3:20518241-20518260 MS.gene58827:CDS 25.0%
! GATAGGAATAATTATGAAAG+AGG + chr7.3:20519236-20519255 MS.gene58827:CDS 25.0%
! TAATTACCAAACAACACAAT+AGG - chr7.3:20519924-20519943 None:intergenic 25.0%
! TAGTAAGATTACGTATGTAA+TGG + chr7.3:20518710-20518729 MS.gene58827:CDS 25.0%
! TGAAAATGAAAATGTGAAAG+AGG + chr7.3:20518374-20518393 MS.gene58827:CDS 25.0%
!! AAAAAACCTATTGTGTTGTT+TGG + chr7.3:20519915-20519934 MS.gene58827:intron 25.0%
!! CTTGATAATCCATTCTATTA+TGG + chr7.3:20519391-20519410 MS.gene58827:CDS 25.0%
!!! TGTTTCTAGGTTTTTGAATT+TGG + chr7.3:20518137-20518156 MS.gene58827:CDS 25.0%
AAACACTGAAAATTCTTACC+GGG + chr7.3:20518242-20518261 MS.gene58827:CDS 30.0%
AATGGTCATATGAATGAATC+TGG + chr7.3:20519167-20519186 MS.gene58827:CDS 30.0%
ATAATTACAATCACCATCCA+AGG + chr7.3:20520142-20520161 MS.gene58827:intron 30.0%
ATAGAGTTCTTGAATTCCTT+GGG + chr7.3:20520017-20520036 MS.gene58827:intron 30.0%
CATCTATAGGGTAAAATTAG+AGG - chr7.3:20519698-20519717 None:intergenic 30.0%
CCAAACTTAAAATCTACCAA+AGG - chr7.3:20519059-20519078 None:intergenic 30.0%
CTAGTTATGTTAGTGATAGA+TGG + chr7.3:20519108-20519127 MS.gene58827:CDS 30.0%
TCAACATCCAAATGTGTATA+GGG + chr7.3:20519529-20519548 MS.gene58827:CDS 30.0%
TCATTCATATGACCATTTAC+AGG - chr7.3:20519164-20519183 None:intergenic 30.0%
TGTATTCAAAAAAGACACCT+TGG - chr7.3:20520162-20520181 None:intergenic 30.0%
TTATCAATCACATGCGAAAT+TGG - chr7.3:20518069-20518088 None:intergenic 30.0%
TTGGATGGTGATTGTAATTA+TGG - chr7.3:20520143-20520162 None:intergenic 30.0%
TTTGAATTTGGATTCAGTTG+CGG + chr7.3:20518149-20518168 MS.gene58827:CDS 30.0%
! AGGTTAAGTGTCTTCAATTT+TGG + chr7.3:20519813-20519832 MS.gene58827:intron 30.0%
! ATTTTACCCTATAGATGGTT+TGG + chr7.3:20519701-20519720 MS.gene58827:intron 30.0%
! CTCTAATTTTACCCTATAGA+TGG + chr7.3:20519696-20519715 MS.gene58827:intron 30.0%
! TAAGCTTACATATTTTGTGC+TGG + chr7.3:20519869-20519888 MS.gene58827:intron 30.0%
! TGAAATTGTCCTGAATTTTC+TGG + chr7.3:20520309-20520328 MS.gene58827:intron 30.0%
! TGGTTTAGTGGATAATGTTT+CGG + chr7.3:20519079-20519098 MS.gene58827:CDS 30.0%
! TGTTTTATGTGCTTCAAAGT+AGG - chr7.3:20520288-20520307 None:intergenic 30.0%
! TTTTGATGGAATTGAAGATG+AGG + chr7.3:20518557-20518576 MS.gene58827:CDS 30.0%
!! AGTTGCTAGTATTAGTTTGT+TGG + chr7.3:20518995-20519014 MS.gene58827:CDS 30.0%
!! GTTGCTAGTATTAGTTTGTT+GGG + chr7.3:20518996-20519015 MS.gene58827:CDS 30.0%
!! TCTGTATTATGCTTTTTGGT+GGG + chr7.3:20519027-20519046 MS.gene58827:CDS 30.0%
!! TTCTGTATTATGCTTTTTGG+TGG + chr7.3:20519026-20519045 MS.gene58827:CDS 30.0%
!!! ACAATAGGTTTTTTACCAAC+GGG - chr7.3:20519909-20519928 None:intergenic 30.0%
!!! CACAATAGGTTTTTTACCAA+CGG - chr7.3:20519910-20519929 None:intergenic 30.0%
!!! CCTTTGGTAGATTTTAAGTT+TGG + chr7.3:20519056-20519075 MS.gene58827:CDS 30.0%
!!! TTGGTAGATTTTAAGTTTGG+TGG + chr7.3:20519059-20519078 MS.gene58827:CDS 30.0%
!!! TTGGTGATTTTTGAGTCTTT+GGG + chr7.3:20519832-20519851 MS.gene58827:intron 30.0%
!!! TTTGGTGATTTTTGAGTCTT+TGG + chr7.3:20519831-20519850 MS.gene58827:intron 30.0%
!!! TTTTAAGTTTGGTGGTTTAG+TGG + chr7.3:20519067-20519086 MS.gene58827:CDS 30.0%
AAAGAGGATGTGAAATTGGA+GGG + chr7.3:20518390-20518409 MS.gene58827:CDS 35.0%
AAGTATGTGACTTACTCATG+TGG + chr7.3:20520716-20520735 MS.gene58827:CDS 35.0%
ACCATAGATGATTTATTGCC+GGG - chr7.3:20520587-20520606 None:intergenic 35.0%
AGAAATACCAAATCTCCCAT+TGG - chr7.3:20519216-20519235 None:intergenic 35.0%
ATAGGGTAAAATTAGAGGAC+AGG - chr7.3:20519693-20519712 None:intergenic 35.0%
ATCATGGAACCAGAAAATTC+AGG - chr7.3:20520321-20520340 None:intergenic 35.0%
ATGAATGAATCTGGAAGGAA+TGG + chr7.3:20519176-20519195 MS.gene58827:CDS 35.0%
ATTGCTTAAGCATGATTGAG+AGG - chr7.3:20520216-20520235 None:intergenic 35.0%
ATTTCTGCAACAAGTTTCCT+TGG + chr7.3:20519964-20519983 MS.gene58827:intron 35.0%
CCTTATGTTGTTAAACCTCA+AGG + chr7.3:20518864-20518883 MS.gene58827:CDS 35.0%
CCTTGAGGTTTAACAACATA+AGG - chr7.3:20518867-20518886 None:intergenic 35.0%
CTATAATATGTGCTACCCAA+AGG - chr7.3:20519795-20519814 None:intergenic 35.0%
CTTTGGGTAGCACATATTAT+AGG + chr7.3:20519793-20519812 MS.gene58827:intron 35.0%
GAAATACCAAATCTCCCATT+GGG - chr7.3:20519215-20519234 None:intergenic 35.0%
GAACTCTATCTAACCAAGTT+TGG - chr7.3:20520006-20520025 None:intergenic 35.0%
GACCATAGATGATTTATTGC+CGG - chr7.3:20520588-20520607 None:intergenic 35.0%
GATAGAGTTCTTGAATTCCT+TGG + chr7.3:20520016-20520035 MS.gene58827:intron 35.0%
GGAATAATTATGAAAGAGGG+AGG + chr7.3:20519240-20519259 MS.gene58827:CDS 35.0%
GTCAACATCCAAATGTGTAT+AGG + chr7.3:20519528-20519547 MS.gene58827:CDS 35.0%
GTCATATGAATGAATCTGGA+AGG + chr7.3:20519171-20519190 MS.gene58827:CDS 35.0%
TAGGTGAAGAAATGAATGAG+CGG + chr7.3:20519267-20519286 MS.gene58827:CDS 35.0%
TGAATGAATCTGGAAGGAAT+GGG + chr7.3:20519177-20519196 MS.gene58827:CDS 35.0%
TGGTTCAACTAAAACCTTGA+AGG + chr7.3:20519583-20519602 MS.gene58827:CDS 35.0%
TGTGAAAGAGGATGTGAAAT+TGG + chr7.3:20518386-20518405 MS.gene58827:CDS 35.0%
TTCAAAAAAGACACCTTGGA+TGG - chr7.3:20520158-20520177 None:intergenic 35.0%
TTCAAAAACCTAGAAACACC+AGG - chr7.3:20518135-20518154 None:intergenic 35.0%
TTCCTACCAAACCATCTATA+GGG - chr7.3:20519710-20519729 None:intergenic 35.0%
TTGAAGAAGGAGTCAAATGA+AGG - chr7.3:20517919-20517938 None:intergenic 35.0%
TTGCTTCTTTGTATGTTCCT+AGG + chr7.3:20519339-20519358 MS.gene58827:CDS 35.0%
TTTCAACAACAAGCTCAATC+TGG + chr7.3:20518534-20518553 MS.gene58827:CDS 35.0%
TTTCAGATGTCTTCCAAACT+TGG + chr7.3:20519990-20520009 MS.gene58827:intron 35.0%
! AAAAGTGCAATGATTAGTGG+AGG - chr7.3:20520083-20520102 None:intergenic 35.0%
! ACCAAAAGTGCAATGATTAG+TGG - chr7.3:20520086-20520105 None:intergenic 35.0%
! CCAATCACATTTTTGCCTTT+GGG + chr7.3:20519777-20519796 MS.gene58827:intron 35.0%
! GGAGATTTGGTATTTCTGAT+AGG + chr7.3:20519219-20519238 MS.gene58827:CDS 35.0%
! GGTTTAGTGGATAATGTTTC+GGG + chr7.3:20519080-20519099 MS.gene58827:CDS 35.0%
! GTGTTTTCATTTTCAGTCTC+CGG - chr7.3:20518228-20518247 None:intergenic 35.0%
! GTTAGTGATAGATGGTTCTA+TGG + chr7.3:20519116-20519135 MS.gene58827:CDS 35.0%
! TCCAATCACATTTTTGCCTT+TGG + chr7.3:20519776-20519795 MS.gene58827:intron 35.0%
! TCCACTAATCATTGCACTTT+TGG + chr7.3:20520082-20520101 MS.gene58827:intron 35.0%
! TTAACAACATAAGGAGCACA+GGG - chr7.3:20518858-20518877 None:intergenic 35.0%
! TTTAACAACATAAGGAGCAC+AGG - chr7.3:20518859-20518878 None:intergenic 35.0%
AAAGATGAAACATCCCTCTC+CGG - chr7.3:20518351-20518370 None:intergenic 40.0%
AAGAAGGAGAAGAGTGAAAC+TGG + chr7.3:20519452-20519471 MS.gene58827:CDS 40.0%
ACACTTAGGCAACAATTGAG+TGG + chr7.3:20518744-20518763 MS.gene58827:CDS 40.0%
ACATACAAAGAAGCAAGCAG+TGG - chr7.3:20519335-20519354 None:intergenic 40.0%
AGACTTCATATGGTCCTTCA+AGG - chr7.3:20519600-20519619 None:intergenic 40.0%
AGAGGACAGGCAATGATTAA+AGG - chr7.3:20519680-20519699 None:intergenic 40.0%
AGATAATCATCAGTCTCCGA+CGG - chr7.3:20518015-20518034 None:intergenic 40.0%
ATCGAACCTCGAAATGTAGT+AGG + chr7.3:20518475-20518494 MS.gene58827:CDS 40.0%
ATGAAGTCTTCTGCAACTGA+TGG + chr7.3:20519611-20519630 MS.gene58827:CDS 40.0%
ATTCACCTGCAATTCCTTCA+CGG - chr7.3:20519655-20519674 None:intergenic 40.0%
CAAACATAAGCTGTCACCAA+AGG - chr7.3:20520758-20520777 None:intergenic 40.0%
CAGTTGCAGAAGACTTCATA+TGG - chr7.3:20519610-20519629 None:intergenic 40.0%
CCATAGATGATTTATTGCCG+GGG - chr7.3:20520586-20520605 None:intergenic 40.0%
CCCAAAGGCAAAAATGTGAT+TGG - chr7.3:20519780-20519799 None:intergenic 40.0%
CCTGAAGTTGGAAGAAATAG+AGG + chr7.3:20519506-20519525 MS.gene58827:CDS 40.0%
CTGAAAATTCTTACCGGGAA+CGG + chr7.3:20518247-20518266 MS.gene58827:CDS 40.0%
CTGATGGTAAAATGCAACAG+TGG + chr7.3:20519627-20519646 MS.gene58827:CDS 40.0%
GAAAGAGGATGTGAAATTGG+AGG + chr7.3:20518389-20518408 MS.gene58827:CDS 40.0%
GAATGAATCTGGAAGGAATG+GGG + chr7.3:20519178-20519197 MS.gene58827:CDS 40.0%
GATCTCGATTTCAACGACTA+CGG + chr7.3:20517868-20517887 MS.gene58827:CDS 40.0%
GCAGAAACCCTATACACATT+TGG - chr7.3:20519539-20519558 None:intergenic 40.0%
GCTCTTCAGTTATGTTCAGA+CGG - chr7.3:20520535-20520554 None:intergenic 40.0%
GTTCCTACCAAACCATCTAT+AGG - chr7.3:20519711-20519730 None:intergenic 40.0%
TATGAAAGAGGGAGGAAACT+AGG + chr7.3:20519248-20519267 MS.gene58827:CDS 40.0%
TCTCACTGGCCATAATAGAA+TGG - chr7.3:20519403-20519422 None:intergenic 40.0%
TGAAAATTCTTACCGGGAAC+GGG + chr7.3:20518248-20518267 MS.gene58827:CDS 40.0%
TGTTAAACCTCAAGGTTCTC+AGG + chr7.3:20518872-20518891 MS.gene58827:CDS 40.0%
TGTTCCTAGGAATGATAAGC+TGG + chr7.3:20519352-20519371 MS.gene58827:CDS 40.0%
TTAAAGGCTGTGATCTGAAG+AGG + chr7.3:20518421-20518440 MS.gene58827:CDS 40.0%
TTCACCAGCTTATCATTCCT+AGG - chr7.3:20519359-20519378 None:intergenic 40.0%
TTGAAATCGAGATCAGAGGA+CGG - chr7.3:20517862-20517881 None:intergenic 40.0%
TTGAAGATGAGGATGAGAAG+AGG + chr7.3:20518568-20518587 MS.gene58827:CDS 40.0%
! ATTGATAATTCGCCGGAATC+AGG + chr7.3:20518081-20518100 MS.gene58827:CDS 40.0%
! CCTCTATTTCTTCCAACTTC+AGG - chr7.3:20519509-20519528 None:intergenic 40.0%
! GAAGACATCTGAAACAACCA+AGG - chr7.3:20519984-20520003 None:intergenic 40.0%
! GATAGATGGTTCTATGGTCA+TGG + chr7.3:20519122-20519141 MS.gene58827:CDS 40.0%
! GCATGTGATTGATAATTCGC+CGG + chr7.3:20518074-20518093 MS.gene58827:CDS 40.0%
! TAGTGGATAATGTTTCGGGT+AGG + chr7.3:20519084-20519103 MS.gene58827:CDS 40.0%
! TTTTTACCAACGGGTAACCA+AGG - chr7.3:20519900-20519919 None:intergenic 40.0%
!! GGTGGAATTTTGACGGTTTT+CGG - chr7.3:20520450-20520469 None:intergenic 40.0%
!! TACCCTATAGATGGTTTGGT+AGG + chr7.3:20519705-20519724 MS.gene58827:intron 40.0%
!! TGACAGTGGTGGAATTTTGA+CGG - chr7.3:20520457-20520476 None:intergenic 40.0%
!! TTGGAGGGAAGTGTTGTTAA+AGG + chr7.3:20518405-20518424 MS.gene58827:CDS 40.0%
!!! ATTTTCTGGTTCCATGATGC+AGG + chr7.3:20520323-20520342 MS.gene58827:intron 40.0%
!!! ATTTTGTGCTGGTTGTTCCT+TGG + chr7.3:20519880-20519899 MS.gene58827:intron 40.0%
!!! CAACATGAAGGCGTTGTTTT+CGG - chr7.3:20520189-20520208 None:intergenic 40.0%
!!! CAAGCTCAATCTGGTTTTGA+TGG + chr7.3:20518543-20518562 MS.gene58827:CDS 40.0%
AAAATCCGAGAGAGCGACTT+CGG - chr7.3:20520671-20520690 None:intergenic 45.0%
AAAGGAACCTGAGAACCTTG+AGG - chr7.3:20518882-20518901 None:intergenic 45.0%
AATAGTTCCAGCAACAGCAG+TGG + chr7.3:20520412-20520431 MS.gene58827:CDS 45.0%
ACGTGATTTCGAAATCGCCA+AGG - chr7.3:20517966-20517985 None:intergenic 45.0%
AGATAATCTCCCAATCCCGT+CGG + chr7.3:20517996-20518015 MS.gene58827:CDS 45.0%
AGGATGAGAAGAGGAAAGCA+AGG + chr7.3:20518577-20518596 MS.gene58827:CDS 45.0%
ATAGGGTTTCTGCTGAACAG+AGG + chr7.3:20519546-20519565 MS.gene58827:CDS 45.0%
ATATTTGCCACTGCTGTTGC+TGG - chr7.3:20520422-20520441 None:intergenic 45.0%
ATCAGAGTCCTGGTGTTTCT+AGG + chr7.3:20518124-20518143 MS.gene58827:CDS 45.0%
ATCGCCAAGGAAATCGAGAT+CGG - chr7.3:20517953-20517972 None:intergenic 45.0%
ATGGCATCTCAAGATGCTCA+GGG + chr7.3:20519428-20519447 MS.gene58827:CDS 45.0%
CACATGCCAGAACTTAGCAT+TGG - chr7.3:20520350-20520369 None:intergenic 45.0%
CCCCGGCAATAAATCATCTA+TGG + chr7.3:20520583-20520602 MS.gene58827:CDS 45.0%
CTGTCACCAAAGGAGAAACA+CGG - chr7.3:20520748-20520767 None:intergenic 45.0%
CTTAGGCAACAATTGAGTGG+TGG + chr7.3:20518747-20518766 MS.gene58827:CDS 45.0%
CTTGTTGATCCCAAAGAGGT+AGG + chr7.3:20520617-20520636 MS.gene58827:CDS 45.0%
GAACCTCGAAATGTAGTAGG+AGG + chr7.3:20518478-20518497 MS.gene58827:CDS 45.0%
GAGCTGGAGAAACATCAAAC+TGG - chr7.3:20520385-20520404 None:intergenic 45.0%
GAGTGAGAGTAAGAAGAGTG+AGG + chr7.3:20518959-20518978 MS.gene58827:CDS 45.0%
GATAAGCTGGTGAAGATCGA+TGG + chr7.3:20519365-20519384 MS.gene58827:CDS 45.0%
GATAATCATCAGTCTCCGAC+GGG - chr7.3:20518014-20518033 None:intergenic 45.0%
GCTTAAGCATGATTGAGAGG+TGG - chr7.3:20520213-20520232 None:intergenic 45.0%
GGAGTCAAATGAAGGAAGAG+CGG - chr7.3:20517911-20517930 None:intergenic 45.0%
GGGAAGATTTGGCCTGTAAA+TGG + chr7.3:20519149-20519168 MS.gene58827:CDS 45.0%
GGGATCTGAATCGTTGAAGA+AGG - chr7.3:20517932-20517951 None:intergenic 45.0%
GTATGAAGCGATGAATTCGC+CGG + chr7.3:20518329-20518348 MS.gene58827:CDS 45.0%
GTCGTTGAAATCGAGATCAG+AGG - chr7.3:20517866-20517885 None:intergenic 45.0%
GTGCTTCAAAGTAGGCAAAC+AGG - chr7.3:20520280-20520299 None:intergenic 45.0%
TAGATGATTTATTGCCGGGG+AGG - chr7.3:20520583-20520602 None:intergenic 45.0%
TCAAGGTTCTCAGGTTCCTT+TGG + chr7.3:20518881-20518900 MS.gene58827:CDS 45.0%
TGGCACATATGCCTTATGCA+TGG + chr7.3:20518829-20518848 MS.gene58827:CDS 45.0%
TGGCTGAGAATGCTACACTT+AGG + chr7.3:20518730-20518749 MS.gene58827:CDS 45.0%
TGTCACCAAAGGAGAAACAC+GGG - chr7.3:20520747-20520766 None:intergenic 45.0%
TTGAGAGGTGGACAACATGA+AGG - chr7.3:20520201-20520220 None:intergenic 45.0%
TTTGATGTTTCTCCAGCTCC+TGG + chr7.3:20520386-20520405 MS.gene58827:CDS 45.0%
! AGATGGTTCTATGGTCATGG+TGG + chr7.3:20519125-20519144 MS.gene58827:CDS 45.0%
! ATATGTGCCATTCCTGGATG+CGG - chr7.3:20518819-20518838 None:intergenic 45.0%
! GAGAAAGTGCACAGCTTTCA+AGG + chr7.3:20518613-20518632 MS.gene58827:CDS 45.0%
! GCATTGGCAATTCCTGAAGT+TGG + chr7.3:20519494-20519513 MS.gene58827:CDS 45.0%
! TAAGGCATATGTGCCATTCC+TGG - chr7.3:20518825-20518844 None:intergenic 45.0%
! TCATTTTCAGTCTCCGGTGA+CGG - chr7.3:20518222-20518241 None:intergenic 45.0%
! TGTGCTTGTTGATCCCAAAG+AGG + chr7.3:20520613-20520632 MS.gene58827:CDS 45.0%
! TTAGCATTGGACCTGCATCA+TGG - chr7.3:20520337-20520356 None:intergenic 45.0%
!! AGAGGAAAGCAAGGTTGATG+AGG + chr7.3:20518586-20518605 MS.gene58827:CDS 45.0%
!! CGGAAAGACTCGAGTTGTTT+TGG + chr7.3:20519287-20519306 MS.gene58827:CDS 45.0%
!! GAGGAAGAAGCATTATGTGG+AGG + chr7.3:20518638-20518657 MS.gene58827:CDS 45.0%
!! GCAGAGGAAGAAGCATTATG+TGG + chr7.3:20518635-20518654 MS.gene58827:CDS 45.0%
!!! TTTTGGTGGGCTTGTTCCTT+TGG + chr7.3:20519040-20519059 MS.gene58827:CDS 45.0%
AAATCGAGATCAGAGGACGG+TGG - chr7.3:20517859-20517878 None:intergenic 50.0%
AACACCAGGACTCTGATCAC+CGG - chr7.3:20518121-20518140 None:intergenic 50.0%
AACCAACTCAGAGACTCCCA+AGG - chr7.3:20520036-20520055 None:intergenic 50.0%
AACCTCGGAATCGGAACCAA+AGG - chr7.3:20518900-20518919 None:intergenic 50.0%
AGATGCCATGGCTTTCTCAC+TGG - chr7.3:20519417-20519436 None:intergenic 50.0%
ATCGCCATCTCCTACCTCTT+TGG - chr7.3:20520630-20520649 None:intergenic 50.0%
ATCTGGAAGGAATGGGGAAG+CGG + chr7.3:20519184-20519203 MS.gene58827:CDS 50.0%
ATGATTTATTGCCGGGGAGG+TGG - chr7.3:20520580-20520599 None:intergenic 50.0%
ATGCAACAGTGGTTCCGTGA+AGG + chr7.3:20519638-20519657 MS.gene58827:CDS 50.0%
CATGATGCATGCAACGCTGA+CGG + chr7.3:20518039-20518058 MS.gene58827:CDS 50.0%
CATGGCATCTCAAGATGCTC+AGG + chr7.3:20519427-20519446 MS.gene58827:CDS 50.0%
CGACCTCCTACTACATTTCG+AGG - chr7.3:20518484-20518503 None:intergenic 50.0%
CTCGATTTCAACGACTACGG+TGG + chr7.3:20517871-20517890 MS.gene58827:CDS 50.0%
CTGAGCATCTTGAGATGCCA+TGG - chr7.3:20519429-20519448 None:intergenic 50.0%
GAACGGGAAGAGTCTTCGAA+TGG + chr7.3:20518264-20518283 MS.gene58827:CDS 50.0%
GATCCCAAAGAGGTAGGAGA+TGG + chr7.3:20520623-20520642 MS.gene58827:CDS 50.0%
GATCCCGATCTCGATTTCCT+TGG + chr7.3:20517946-20517965 MS.gene58827:CDS 50.0%
GCAGGTCCAATGCTAAGTTC+TGG + chr7.3:20520341-20520360 MS.gene58827:intron 50.0%
GCATTATGTGGAGGAGCTTG+AGG + chr7.3:20518647-20518666 MS.gene58827:CDS 50.0%
GGAAGAGTCTTCGAATGGAC+CGG + chr7.3:20518269-20518288 MS.gene58827:CDS 50.0%
GGAGAAGAGTGAAACTGGTC+TGG + chr7.3:20519457-20519476 MS.gene58827:CDS 50.0%
GGCAAACAGGCACATATGTC+AGG - chr7.3:20520267-20520286 None:intergenic 50.0%
GGGTTTCCCAATGGGAGATT+TGG + chr7.3:20519206-20519225 MS.gene58827:CDS 50.0%
GGTTTCTGCTGAACAGAGGA+GGG + chr7.3:20519550-20519569 MS.gene58827:CDS 50.0%
GTAGGAGATGGCGATGTTGA+TGG + chr7.3:20520635-20520654 MS.gene58827:CDS 50.0%
GTCACCAAAGGAGAAACACG+GGG - chr7.3:20520746-20520765 None:intergenic 50.0%
GTTATGTTCAGACGGCTTCC+AGG - chr7.3:20520527-20520546 None:intergenic 50.0%
GTTCCTGCTCTTGTTGACAG+TGG - chr7.3:20520471-20520490 None:intergenic 50.0%
GTTGCTGGAACTATTGCTCC+AGG - chr7.3:20520407-20520426 None:intergenic 50.0%
GTTGTTCCTTGGTTACCCGT+TGG + chr7.3:20519891-20519910 MS.gene58827:intron 50.0%
TAGTAGGAGGTCGTTGTCGA+TGG + chr7.3:20518491-20518510 MS.gene58827:CDS 50.0%
TATGGCCAGTGAGAAAGCCA+TGG + chr7.3:20519409-20519428 MS.gene58827:CDS 50.0%
TCAAGATGCTCAGGGGAAGA+AGG + chr7.3:20519436-20519455 MS.gene58827:CDS 50.0%
TCAGGTGCTTCTGTGGTTTC+CGG + chr7.3:20518099-20518118 MS.gene58827:CDS 50.0%
TCGCCAAGGAAATCGAGATC+GGG - chr7.3:20517952-20517971 None:intergenic 50.0%
TCGCCATCTCCTACCTCTTT+GGG - chr7.3:20520629-20520648 None:intergenic 50.0%
TCTTCCCCGTGTTTCTCCTT+TGG + chr7.3:20520739-20520758 MS.gene58827:CDS 50.0%
TGAGGCTTCAACCTCGGAAT+CGG - chr7.3:20518909-20518928 None:intergenic 50.0%
TGCCAATGCAGAATCCCGAA+TGG - chr7.3:20519483-20519502 None:intergenic 50.0%
TGGCATCTCAAGATGCTCAG+GGG + chr7.3:20519429-20519448 MS.gene58827:CDS 50.0%
TGGTTCCGTGAAGGAATTGC+AGG + chr7.3:20519647-20519666 MS.gene58827:CDS 50.0%
TTCCTTGGGAGTCTCTGAGT+TGG + chr7.3:20520031-20520050 MS.gene58827:intron 50.0%
! AATGGACCGGTTTCGTCTCA+GGG + chr7.3:20518282-20518301 MS.gene58827:CDS 50.0%
! GGTTCTATGGTCATGGTGGA+GGG + chr7.3:20519129-20519148 MS.gene58827:CDS 50.0%
! GTCATGGTGGAGGGAAGATT+TGG + chr7.3:20519138-20519157 MS.gene58827:CDS 50.0%
! GTGAAACTGGTCTGGCCATT+CGG + chr7.3:20519465-20519484 MS.gene58827:CDS 50.0%
! TGAAACTGGTCTGGCCATTC+GGG + chr7.3:20519466-20519485 MS.gene58827:CDS 50.0%
! TGGTTCTATGGTCATGGTGG+AGG + chr7.3:20519128-20519147 MS.gene58827:CDS 50.0%
! TTCCTTTGGTTCCGATTCCG+AGG + chr7.3:20518895-20518914 MS.gene58827:CDS 50.0%
!! ACCACAGAAGCACCTGATTC+CGG - chr7.3:20518096-20518115 None:intergenic 50.0%
!! AGTTCTGAGCTTTCCGTCAC+CGG + chr7.3:20518206-20518225 MS.gene58827:CDS 50.0%
!! TGGCGATGTTGATGGCATGA+TGG + chr7.3:20520643-20520662 MS.gene58827:CDS 50.0%
AAGCGATGAATTCGCCGGAG+AGG + chr7.3:20518334-20518353 MS.gene58827:CDS 55.0%
AGCGATGAATTCGCCGGAGA+GGG + chr7.3:20518335-20518354 MS.gene58827:CDS 55.0%
CATCAGTCTCCGACGGGATT+GGG - chr7.3:20518008-20518027 None:intergenic 55.0%
CCACCACTGTCAACAAGAGC+AGG + chr7.3:20520465-20520484 MS.gene58827:CDS 55.0%
CCTGCTCTTGTTGACAGTGG+TGG - chr7.3:20520468-20520487 None:intergenic 55.0%
GCTGAACAGAGGAGGGCTAT+TGG + chr7.3:20519557-20519576 MS.gene58827:CDS 55.0%
GGAACTATTGCTCCAGGAGC+TGG - chr7.3:20520401-20520420 None:intergenic 55.0%
GGCCATTCGGGATTCTGCAT+TGG + chr7.3:20519478-20519497 MS.gene58827:CDS 55.0%
GGCTTCCAGGAAGTGGATCT+GGG - chr7.3:20520514-20520533 None:intergenic 55.0%
GGGTTTCTGCTGAACAGAGG+AGG + chr7.3:20519549-20519568 MS.gene58827:CDS 55.0%
GTCGTTGTCGATGGAGAGGA+CGG + chr7.3:20518500-20518519 MS.gene58827:CDS 55.0%
GTGCACAGCTTTCAAGGCAG+AGG + chr7.3:20518619-20518638 MS.gene58827:CDS 55.0%
GTTGGCTGAGGCTTCAACCT+CGG - chr7.3:20518915-20518934 None:intergenic 55.0%
GTTTCCGGTGATCAGAGTCC+TGG + chr7.3:20518114-20518133 MS.gene58827:CDS 55.0%
TCAGAGGACGGTGGTTGTTC+CGG - chr7.3:20517850-20517869 None:intergenic 55.0%
TCATCAGTCTCCGACGGGAT+TGG - chr7.3:20518009-20518028 None:intergenic 55.0%
TCCAGGAAGTGGATCTGGGA+GGG - chr7.3:20520510-20520529 None:intergenic 55.0%
TCGAAGACTCTTCCCGTTCC+CGG - chr7.3:20518263-20518282 None:intergenic 55.0%
TCTGGAAGGAATGGGGAAGC+GGG + chr7.3:20519185-20519204 MS.gene58827:CDS 55.0%
TTCAGACGGCTTCCAGGAAG+TGG - chr7.3:20520521-20520540 None:intergenic 55.0%
TTCCAGGAAGTGGATCTGGG+AGG - chr7.3:20520511-20520530 None:intergenic 55.0%
TTGCCTAAAGACCACCTCCC+CGG + chr7.3:20520566-20520585 MS.gene58827:CDS 55.0%
TTGTTCCGGCGGTGGTTGAA+CGG - chr7.3:20517836-20517855 None:intergenic 55.0%
! ACCGGTTTCGTCTCAGGGAT+CGG + chr7.3:20518287-20518306 MS.gene58827:CDS 55.0%
! GAATGGACCGGTTTCGTCTC+AGG + chr7.3:20518281-20518300 MS.gene58827:CDS 55.0%
! GCACAGGGCATCCATGCATA+AGG - chr7.3:20518843-20518862 None:intergenic 55.0%
! GTCGCTCTCTCGGATTTTCG+TGG + chr7.3:20520673-20520692 MS.gene58827:CDS 55.0%
ACCCTCCCAGATCCACTTCC+TGG + chr7.3:20520506-20520525 MS.gene58827:CDS 60.0%
CCCGATCCCTGAGACGAAAC+CGG - chr7.3:20518291-20518310 None:intergenic 60.0%
CCGGTTTCGTCTCAGGGATC+GGG + chr7.3:20518288-20518307 MS.gene58827:CDS 60.0%
CCTGGATGCGGTGGGTACAT+CGG - chr7.3:20518807-20518826 None:intergenic 60.0%
CGGCTTCCAGGAAGTGGATC+TGG - chr7.3:20520515-20520534 None:intergenic 60.0%
CGGTTTCGTCTCAGGGATCG+GGG + chr7.3:20518289-20518308 MS.gene58827:CDS 60.0%
CTCTTCGAAGCCGCAGCAGT+TGG - chr7.3:20518933-20518952 None:intergenic 60.0%
CTGGAAGGAATGGGGAAGCG+GGG + chr7.3:20519186-20519205 MS.gene58827:CDS 60.0%
CTGGATGCGGTGGGTACATC+GGG - chr7.3:20518806-20518825 None:intergenic 60.0%
GAAGCCTCAGCCAACTGCTG+CGG + chr7.3:20518920-20518939 MS.gene58827:CDS 60.0%
GAAGTGGATCTGGGAGGGTG+TGG - chr7.3:20520505-20520524 None:intergenic 60.0%
GCCGGAATCAGGTGCTTCTG+TGG + chr7.3:20518092-20518111 MS.gene58827:CDS 60.0%
GGAGGTCGTTGTCGATGGAG+AGG + chr7.3:20518496-20518515 MS.gene58827:CDS 60.0%
GGGAAGCGGGGTTTCCCAAT+GGG + chr7.3:20519198-20519217 MS.gene58827:CDS 60.0%
GTACCCACCGCATCCAGGAA+TGG + chr7.3:20518809-20518828 MS.gene58827:CDS 60.0%
TCGTCTCAGGGATCGGGGAA+CGG + chr7.3:20518294-20518313 MS.gene58827:CDS 60.0%
TCTCACCGTTCAACCACCGC+CGG + chr7.3:20517828-20517847 MS.gene58827:CDS 60.0%
TGGATGCGGTGGGTACATCG+GGG - chr7.3:20518805-20518824 None:intergenic 60.0%
TGGCTCCGAAGTCGCTCTCT+CGG + chr7.3:20520663-20520682 MS.gene58827:CDS 60.0%
TTGCCGGGGAGGTGGTCTTT+AGG - chr7.3:20520572-20520591 None:intergenic 60.0%
! GTGCCATTCCTGGATGCGGT+GGG - chr7.3:20518815-20518834 None:intergenic 60.0%
! TGTGCCATTCCTGGATGCGG+TGG - chr7.3:20518816-20518835 None:intergenic 60.0%
!! CAGAGGAGGGCTATTGGCTC+TGG + chr7.3:20519563-20519582 MS.gene58827:CDS 60.0%
CCGATGTACCCACCGCATCC+AGG + chr7.3:20518804-20518823 MS.gene58827:CDS 65.0%
GAAGCCGCAGCAGTTGGCTG+AGG - chr7.3:20518927-20518946 None:intergenic 65.0%
GAGGACGGTGGTTGTTCCGG+CGG - chr7.3:20517847-20517866 None:intergenic 65.0%
GGATCGGGGAACGGTGGATC+GGG + chr7.3:20518303-20518322 MS.gene58827:CDS 65.0%
GGGATCGGGGAACGGTGGAT+CGG + chr7.3:20518302-20518321 MS.gene58827:CDS 65.0%
GGGGAAGCGGGGTTTCCCAA+TGG + chr7.3:20519197-20519216 MS.gene58827:CDS 65.0%
TCTCAGGGATCGGGGAACGG+TGG + chr7.3:20518297-20518316 MS.gene58827:CDS 65.0%
TGTCCACCACCACCACCACC+TGG + chr7.3:20518777-20518796 MS.gene58827:CDS 65.0%
! GTGGATCTGGGAGGGTGTGG+AGG - chr7.3:20520502-20520521 None:intergenic 65.0%
! TACATCGGGGCACCAGGTGG+TGG - chr7.3:20518792-20518811 None:intergenic 65.0%
GACGGTGGTTGTTCCGGCGG+TGG - chr7.3:20517844-20517863 None:intergenic 70.0%
! ATCGGGGCACCAGGTGGTGG+TGG - chr7.3:20518789-20518808 None:intergenic 70.0%
! GGGTACATCGGGGCACCAGG+TGG - chr7.3:20518795-20518814 None:intergenic 70.0%
! GGTGGGTACATCGGGGCACC+AGG - chr7.3:20518798-20518817 None:intergenic 70.0%
!! GCACCAGGTGGTGGTGGTGG+TGG - chr7.3:20518783-20518802 None:intergenic 70.0%
!! GGGGCACCAGGTGGTGGTGG+TGG - chr7.3:20518786-20518805 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 20517814 20520778 20517814 ID=MS.gene58827
chr7.3 mRNA 20517814 20520778 20517814 ID=MS.gene58827.t1;Parent=MS.gene58827
chr7.3 exon 20517814 20519668 20517814 ID=MS.gene58827.t1.exon1;Parent=MS.gene58827.t1
chr7.3 CDS 20517814 20519668 20517814 ID=cds.MS.gene58827.t1;Parent=MS.gene58827.t1
chr7.3 exon 20520345 20520778 20520345 ID=MS.gene58827.t1.exon2;Parent=MS.gene58827.t1
chr7.3 CDS 20520345 20520778 20520345 ID=cds.MS.gene58827.t1;Parent=MS.gene58827.t1
Gene Sequence

>MS.gene58827

ATGGCTGATTCAATTCTCACCGTTCAACCACCGCCGGAACAACCACCGTCCTCTGATCTCGATTTCAACGACTACGGTGGCGAATTCAACAACACCGCTCTTCCTTCATTTGACTCCTTCTTCAACGATTCAGATCCCGATCTCGATTTCCTTGGCGATTTCGAAATCACGTTTGATGATCTAGATAATCTCCCAATCCCGTCGGAGACTGATGATTATCTTTTTCATGATGCATGCAACGCTGACGGTGTTCCAATTTCGCATGTGATTGATAATTCGCCGGAATCAGGTGCTTCTGTGGTTTCCGGTGATCAGAGTCCTGGTGTTTCTAGGTTTTTGAATTTGGATTCAGTTGCGGATGATGATGATAAAGAGAATTCAGTTGATGTGAAAGTTCTGAGCTTTCCGTCACCGGAGACTGAAAATGAAAACACTGAAAATTCTTACCGGGAACGGGAAGAGTCTTCGAATGGACCGGTTTCGTCTCAGGGATCGGGGAACGGTGGATCGGGTGTGTATGAAGCGATGAATTCGCCGGAGAGGGATGTTTCATCTTTTCATGAAAATGAAAATGTGAAAGAGGATGTGAAATTGGAGGGAAGTGTTGTTAAAGGCTGTGATCTGAAGAGGAAGAAAGAAAATAGCTATGAAAGTGCTGAGAATCGAACCTCGAAATGTAGTAGGAGGTCGTTGTCGATGGAGAGGACGGAACAACAACAGTTTCAACAACAAGCTCAATCTGGTTTTGATGGAATTGAAGATGAGGATGAGAAGAGGAAAGCAAGGTTGATGAGGAATAGAGAAAGTGCACAGCTTTCAAGGCAGAGGAAGAAGCATTATGTGGAGGAGCTTGAGGAGAAAGTGAGATCAATGCATTCAACAATTACTGATTTAAGTAGTAAGATTACGTATGTAATGGCTGAGAATGCTACACTTAGGCAACAATTGAGTGGTGGTGTGATGTGTCCACCACCACCACCACCTGGTGCCCCGATGTACCCACCGCATCCAGGAATGGCACATATGCCTTATGCATGGATGCCCTGTGCTCCTTATGTTGTTAAACCTCAAGGTTCTCAGGTTCCTTTGGTTCCGATTCCGAGGTTGAAGCCTCAGCCAACTGCTGCGGCTTCGAAGAGTAAAAAGAGTGAGAGTAAGAAGAGTGAGGTGAAAACTAAGAAAGTTGCTAGTATTAGTTTGTTGGGTTTGTTTTTCTGTATTATGCTTTTTGGTGGGCTTGTTCCTTTGGTAGATTTTAAGTTTGGTGGTTTAGTGGATAATGTTTCGGGTAGGTCTAGTTATGTTAGTGATAGATGGTTCTATGGTCATGGTGGAGGGAAGATTTGGCCTGTAAATGGTCATATGAATGAATCTGGAAGGAATGGGGAAGCGGGGTTTCCCAATGGGAGATTTGGTATTTCTGATAGGAATAATTATGAAAGAGGGAGGAAACTAGGTGAAGAAATGAATGAGCGGAAAGACTCGAGTTGTTTTGGTCATCGAGACAATGCTAGCGAGCCACTGCTTGCTTCTTTGTATGTTCCTAGGAATGATAAGCTGGTGAAGATCGATGGAAACTTGATAATCCATTCTATTATGGCCAGTGAGAAAGCCATGGCATCTCAAGATGCTCAGGGGAAGAAGGAGAAGAGTGAAACTGGTCTGGCCATTCGGGATTCTGCATTGGCAATTCCTGAAGTTGGAAGAAATAGAGGTCAACATCCAAATGTGTATAGGGTTTCTGCTGAACAGAGGAGGGCTATTGGCTCTGGTTCAACTAAAACCTTGAAGGACCATATGAAGTCTTCTGCAACTGATGGTAAAATGCAACAGTGGTTCCGTGAAGGAATTGCAGGTGAATATCCTTTAATCATTGCCTGTCCTCTAATTTTACCCTATAGATGGTTTGGTAGGAACGATACATTCTCTTGTGCTAATTATATCTTGTTTTGTTTGTTGCTATCCAATCACATTTTTGCCTTTGGGTAGCACATATTATAGGTTAAGTGTCTTCAATTTTGGTGATTTTTGAGTCTTTGGGATGTTATTTATATTTAAGCTTACATATTTTGTGCTGGTTGTTCCTTGGTTACCCGTTGGTAAAAAACCTATTGTGTTGTTTGGTAATTAAAAACTTGTGTCTTGTAATTATTTCTGCAACAAGTTTCCTTGGTTGTTTCAGATGTCTTCCAAACTTGGTTAGATAGAGTTCTTGAATTCCTTGGGAGTCTCTGAGTTGGTTATATCTGTGCTTAATTAACAAATACCTCCACTAATCATTGCACTTTTGGTTCGTTGTTGTATTTGTGAAATAGCTTTTTTTATGCCATAATTACAATCACCATCCAAGGTGTCTTTTTTGAATACAAGAACCGAAAACAACGCCTTCATGTTGTCCACCTCTCAATCATGCTTAAGCAATATCTTTTGCTTGCTGTACAAGTTTTATGCCTGACATATGTGCCTGTTTGCCTACTTTGAAGCACATAAAACATTGAAATTGTCCTGAATTTTCTGGTTCCATGATGCAGGTCCAATGCTAAGTTCTGGCATGTGTACTGAAGTTTTCCAGTTTGATGTTTCTCCAGCTCCTGGAGCAATAGTTCCAGCAACAGCAGTGGCAAATATTTCTTCCGAAAACCGTCAAAATTCCACCACTGTCAACAAGAGCAGGAACAGAAGAATCCTCCACACCCTCCCAGATCCACTTCCTGGAAGCCGTCTGAACATAACTGAAGAGCGTGCAAGAAATTTGCCTAAAGACCACCTCCCCGGCAATAAATCATCTATGGTCGTTTCTGTGCTTGTTGATCCCAAAGAGGTAGGAGATGGCGATGTTGATGGCATGATGGCTCCGAAGTCGCTCTCTCGGATTTTCGTGGTTGTGTTAATCGACAGTGTCAAGTATGTGACTTACTCATGTGGTCTTCCCCGTGTTTCTCCTTTGGTGACAGCTTATGTTTGA
Protein sequence

>MS.gene58827.t1

MADSILTVQPPPEQPPSSDLDFNDYGGEFNNTALPSFDSFFNDSDPDLDFLGDFEITFDDLDNLPIPSETDDYLFHDACNADGVPISHVIDNSPESGASVVSGDQSPGVSRFLNLDSVADDDDKENSVDVKVLSFPSPETENENTENSYREREESSNGPVSSQGSGNGGSGVYEAMNSPERDVSSFHENENVKEDVKLEGSVVKGCDLKRKKENSYESAENRTSKCSRRSLSMERTEQQQFQQQAQSGFDGIEDEDEKRKARLMRNRESAQLSRQRKKHYVEELEEKVRSMHSTITDLSSKITYVMAENATLRQQLSGGVMCPPPPPPGAPMYPPHPGMAHMPYAWMPCAPYVVKPQGSQVPLVPIPRLKPQPTAAASKSKKSESKKSEVKTKKVASISLLGLFFCIMLFGGLVPLVDFKFGGLVDNVSGRSSYVSDRWFYGHGGGKIWPVNGHMNESGRNGEAGFPNGRFGISDRNNYERGRKLGEEMNERKDSSCFGHRDNASEPLLASLYVPRNDKLVKIDGNLIIHSIMASEKAMASQDAQGKKEKSETGLAIRDSALAIPEVGRNRGQHPNVYRVSAEQRRAIGSGSTKTLKDHMKSSATDGKMQQWFREGIAGPMLSSGMCTEVFQFDVSPAPGAIVPATAVANISSENRQNSTTVNKSRNRRILHTLPDPLPGSRLNITEERARNLPKDHLPGNKSSMVVSVLVDPKEVGDGDVDGMMAPKSLSRIFVVVLIDSVKYVTYSCGLPRVSPLVTAYV