AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58831

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58831.t1 MTR_7g089010 97.351 302 1 5 1 298 1 299 0.0 578
MS.gene58831.t1 MTR_4g064730 44.749 219 63 5 5 181 8 210 1.22e-43 151
MS.gene58831.t1 MTR_4g121020 67.647 68 22 0 85 152 106 173 2.84e-25 105
MS.gene58831.t1 MTR_4g121020 67.647 68 22 0 85 152 210 277 3.20e-25 105
MS.gene58831.t1 MTR_4g121020 67.647 68 22 0 85 152 210 277 3.26e-25 105
MS.gene58831.t1 MTR_2g450070 58.442 77 32 0 89 165 212 288 1.28e-22 98.2
MS.gene58831.t1 MTR_2g450070 58.442 77 32 0 89 165 212 288 1.39e-22 98.2
MS.gene58831.t1 MTR_2g034960 60.294 68 27 0 85 152 206 273 5.02e-22 96.3
MS.gene58831.t1 MTR_3g106220 54.545 77 34 1 77 153 186 261 7.27e-22 95.9
MS.gene58831.t1 MTR_7g117705 63.636 66 21 1 89 154 190 252 3.25e-21 93.6
MS.gene58831.t1 MTR_1g032570 60.938 64 25 0 89 152 130 193 8.69e-21 92.8
MS.gene58831.t1 MTR_1g032570 60.938 64 25 0 89 152 199 262 9.76e-21 92.4
MS.gene58831.t1 MTR_1g013170 65.000 60 21 0 89 148 190 249 3.97e-20 90.5
MS.gene58831.t1 MTR_4g131570 65.574 61 21 0 87 147 200 260 5.64e-20 90.5
MS.gene58831.t1 MTR_7g026400 34.848 132 72 3 81 212 148 265 7.80e-20 89.7
MS.gene58831.t1 MTR_4g131600 54.412 68 31 0 81 148 192 259 2.26e-19 88.6
MS.gene58831.t1 MTR_0450s0040 52.857 70 33 0 87 156 166 235 3.83e-19 86.7
MS.gene58831.t1 MTR_4g131580 65.574 61 21 0 87 147 197 257 4.78e-19 87.4
MS.gene58831.t1 MTR_1g013180 54.930 71 32 0 89 159 341 411 5.46e-19 87.4
MS.gene58831.t1 MTR_3g102600 54.545 66 28 1 89 154 82 145 3.99e-18 80.5
MS.gene58831.t1 MTR_3g102600 54.545 66 28 1 89 154 131 194 6.53e-18 81.3
MS.gene58831.t1 MTR_8g079940 56.164 73 28 1 88 156 207 279 2.20e-17 82.4
MS.gene58831.t1 MTR_3g086100 51.389 72 35 0 89 160 127 198 7.30e-17 80.9
MS.gene58831.t1 MTR_3g086100 51.389 72 35 0 89 160 198 269 9.43e-17 80.9
MS.gene58831.t1 MTR_5g014040 56.667 60 26 0 89 148 310 369 1.56e-16 80.1
MS.gene58831.t1 MTR_4g098870 55.000 60 27 0 89 148 309 368 7.31e-16 78.2
MS.gene58831.t1 MTR_4g098870 58.929 56 23 0 89 144 309 364 7.49e-16 78.2
MS.gene58831.t1 MTR_4g098870 58.929 56 23 0 89 144 329 384 7.50e-16 78.2
MS.gene58831.t1 MTR_4g098870 55.000 60 27 0 89 148 329 388 7.75e-16 78.2
MS.gene58831.t1 MTR_4g098870 55.000 60 27 0 89 148 348 407 8.34e-16 77.8
MS.gene58831.t1 MTR_4g086835 51.515 66 32 0 83 148 267 332 8.63e-16 77.4
MS.gene58831.t1 MTR_4g021760 56.897 58 25 0 89 146 168 225 1.93e-15 75.5
MS.gene58831.t1 MTR_4g078810 51.667 60 29 0 89 148 157 216 2.29e-15 76.3
MS.gene58831.t1 MTR_2g090675 34.351 131 51 3 89 207 160 267 2.84e-15 75.9
MS.gene58831.t1 MTR_3g102590 44.304 79 42 1 89 165 142 220 3.83e-15 73.9
MS.gene58831.t1 MTR_5g014040 52.308 65 26 1 89 148 270 334 1.68e-14 73.9
MS.gene58831.t1 MTR_4g086835 50.746 67 32 1 83 148 267 333 1.76e-14 73.6
MS.gene58831.t1 MTR_5g014040 52.308 65 26 1 89 148 310 374 1.91e-14 73.9
MS.gene58831.t1 MTR_5g017980 52.542 59 28 0 89 147 191 249 4.06e-14 72.0
MS.gene58831.t1 MTR_5g017980 52.542 59 28 0 89 147 192 250 4.63e-14 71.6
MS.gene58831.t1 MTR_4g021790 54.237 59 27 0 89 147 167 225 5.51e-14 70.9
MS.gene58831.t1 MTR_4g113140 52.381 63 29 1 86 147 231 293 8.70e-14 71.6
MS.gene58831.t1 MTR_8g105600 57.895 57 24 0 93 149 190 246 9.66e-14 71.6
MS.gene58831.t1 MTR_5g041350 56.364 55 23 1 90 143 15 69 1.72e-13 70.5
MS.gene58831.t1 MTR_2g086450 55.385 65 27 2 90 153 45 108 3.89e-13 69.3
MS.gene58831.t1 MTR_5g017980 51.667 60 28 1 89 147 192 251 1.16e-12 67.8
MS.gene58831.t1 MTR_6g032990 40.000 95 45 3 90 180 42 128 1.18e-12 68.2
MS.gene58831.t1 MTR_6g444980 60.000 55 21 1 90 143 35 89 1.24e-12 67.8
MS.gene58831.t1 MTR_6g444980 60.000 55 21 1 90 143 35 89 1.27e-12 67.8
MS.gene58831.t1 MTR_2g027800 50.685 73 35 1 90 161 39 111 1.36e-12 67.4
MS.gene58831.t1 MTR_1g090670 46.429 84 41 2 80 159 31 114 1.45e-12 67.0
MS.gene58831.t1 MTR_4g021855 52.542 59 28 0 89 147 167 225 1.69e-12 67.0
MS.gene58831.t1 MTR_7g098250 50.725 69 31 2 90 157 262 328 1.95e-12 67.8
MS.gene58831.t1 MTR_1g093080 50.000 60 29 1 89 147 210 269 2.00e-12 67.4
MS.gene58831.t1 MTR_5g054300 51.667 60 28 1 89 147 197 256 2.57e-12 67.0
MS.gene58831.t1 MTR_1g053830 44.928 69 37 1 76 143 4 72 3.26e-12 62.4
MS.gene58831.t1 MTR_5g027440 56.364 55 23 1 90 143 25 79 3.44e-12 65.9
MS.gene58831.t1 MTR_3g102600 47.170 53 28 0 89 141 82 134 4.43e-12 63.2
MS.gene58831.t1 MTR_2g027800 60.000 55 21 1 90 143 39 93 4.94e-12 65.5
MS.gene58831.t1 MTR_3g102600 47.170 53 28 0 89 141 131 183 6.94e-12 63.5
MS.gene58831.t1 MTR_1g080330 55.556 54 23 1 90 142 175 228 8.42e-12 65.5
MS.gene58831.t1 MTR_1g080330 55.556 54 23 1 90 142 175 228 9.52e-12 64.7
MS.gene58831.t1 MTR_6g045327 48.276 58 30 0 89 146 171 228 1.07e-11 65.1
MS.gene58831.t1 MTR_2g027860 41.667 96 45 2 59 143 8 103 1.19e-11 64.7
MS.gene58831.t1 MTR_4g081710 52.727 55 25 1 90 143 17 71 1.43e-11 64.7
MS.gene58831.t1 MTR_7g115530 53.704 54 24 1 90 142 218 271 1.84e-11 63.9
MS.gene58831.t1 MTR_7g115530 53.704 54 24 1 90 142 218 271 2.56e-11 63.9
MS.gene58831.t1 MTR_7g115530 53.704 54 24 1 90 142 218 271 2.92e-11 63.9
MS.gene58831.t1 MTR_7g088070 44.444 99 41 4 83 169 43 139 3.01e-11 63.2
MS.gene58831.t1 MTR_7g115530 53.704 54 24 1 90 142 218 271 3.39e-11 63.5
MS.gene58831.t1 MTR_8g066310 42.308 104 54 4 43 142 125 226 3.50e-11 63.5
MS.gene58831.t1 MTR_7g088070 51.613 62 29 1 83 143 43 104 7.63e-11 62.0
MS.gene58831.t1 MTR_0223s0040 56.364 55 23 1 89 142 17 71 7.88e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58831.t1 AT3G10760 56.358 346 92 10 1 298 1 335 2.60e-92 277
MS.gene58831.t1 AT5G05090 55.921 304 90 11 1 298 1 266 8.02e-82 248
MS.gene58831.t1 AT2G40970 62.150 214 55 6 1 198 1 204 4.42e-66 207
MS.gene58831.t1 AT3G46640 37.342 316 137 6 5 271 15 318 1.44e-51 172
MS.gene58831.t1 AT3G46640 37.342 316 137 6 5 271 15 318 1.44e-51 172
MS.gene58831.t1 AT3G46640 38.413 315 116 8 5 275 15 295 2.95e-51 171
MS.gene58831.t1 AT5G59570 44.712 208 67 6 12 183 29 224 7.49e-44 152
MS.gene58831.t1 AT5G59570 44.712 208 67 6 12 183 29 224 7.49e-44 152
MS.gene58831.t1 AT3G16857 60.000 75 30 0 78 152 155 229 3.96e-24 102
MS.gene58831.t1 AT3G16857 60.000 75 30 0 78 152 225 299 4.47e-24 102
MS.gene58831.t1 AT3G16857 54.545 88 40 0 65 152 212 299 4.96e-24 102
MS.gene58831.t1 AT5G58080 66.129 62 21 0 87 148 191 252 5.13e-22 96.3
MS.gene58831.t1 AT2G01760 63.077 65 24 0 89 153 134 198 2.39e-21 92.4
MS.gene58831.t1 AT2G01760 63.077 65 24 0 89 153 199 263 6.63e-21 92.0
MS.gene58831.t1 AT2G25180 61.538 65 25 0 89 153 194 258 9.72e-21 92.8
MS.gene58831.t1 AT2G01760 63.077 65 24 0 89 153 227 291 1.11e-20 91.7
MS.gene58831.t1 AT4G31920 61.667 60 23 0 89 148 182 241 4.12e-19 87.8
MS.gene58831.t1 AT5G07210 33.333 144 76 3 81 224 213 336 6.23e-18 84.3
MS.gene58831.t1 AT5G07210 33.333 144 76 3 81 224 221 344 6.82e-18 84.3
MS.gene58831.t1 AT3G62670 38.710 124 66 4 89 204 210 331 4.76e-16 78.2
MS.gene58831.t1 AT5G44190 55.000 60 27 0 89 148 146 205 5.49e-16 77.8
MS.gene58831.t1 AT5G44190 55.000 60 27 0 89 148 146 205 6.48e-16 77.4
MS.gene58831.t1 AT2G20570 55.000 60 27 0 89 148 152 211 4.12e-15 75.5
MS.gene58831.t1 AT2G20570 57.895 57 24 0 92 148 171 227 9.11e-15 74.7
MS.gene58831.t1 AT1G67710 59.322 59 23 1 92 149 121 179 2.05e-14 73.6
MS.gene58831.t1 AT1G67710 59.322 59 23 1 92 149 130 188 2.27e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT4G18020 54.237 59 27 0 90 148 296 354 2.38e-14 73.6
MS.gene58831.t1 AT3G12730 61.818 55 20 1 90 143 23 77 2.54e-14 71.2
MS.gene58831.t1 AT1G67710 59.322 59 23 1 92 149 195 253 2.97e-14 73.2
MS.gene58831.t1 AT2G27070 48.529 68 35 0 81 148 216 283 3.81e-14 73.2
MS.gene58831.t1 AT3G04030 57.407 54 23 0 90 143 45 98 8.07e-14 71.6
MS.gene58831.t1 AT1G79430 61.818 55 20 1 90 143 34 88 2.33e-13 70.1
MS.gene58831.t1 AT5G45580 58.182 55 22 1 90 143 7 61 2.45e-13 68.9
MS.gene58831.t1 AT5G45580 58.182 55 22 1 90 143 23 77 2.66e-13 69.3
MS.gene58831.t1 AT2G03500 53.333 60 27 1 89 147 232 291 3.48e-13 69.7
MS.gene58831.t1 AT3G04030 58.182 55 22 1 90 143 45 99 4.54e-13 69.3
MS.gene58831.t1 AT3G04030 58.182 55 22 1 90 143 45 99 4.85e-13 69.3
MS.gene58831.t1 AT3G24120 60.000 55 21 1 90 143 41 95 4.97e-13 68.6
MS.gene58831.t1 AT3G24120 60.000 55 21 1 90 143 41 95 5.23e-13 68.6
MS.gene58831.t1 AT4G13640 60.000 55 21 1 90 143 37 91 5.71e-13 68.6
MS.gene58831.t1 AT4G13640 60.000 55 21 1 90 143 37 91 5.92e-13 68.2
MS.gene58831.t1 AT5G18240 60.000 55 21 1 90 143 45 99 7.98e-13 68.6
MS.gene58831.t1 AT5G18240 60.000 55 21 1 90 143 45 99 7.98e-13 68.6
MS.gene58831.t1 AT5G18240 60.000 55 21 1 90 143 45 99 8.52e-13 68.6
MS.gene58831.t1 AT5G18240 60.000 55 21 1 90 143 45 99 8.52e-13 68.6
MS.gene58831.t1 AT5G18240 60.000 55 21 1 90 143 45 99 8.62e-13 68.6
MS.gene58831.t1 AT1G13300 51.667 60 28 1 89 147 180 239 1.05e-12 68.2
MS.gene58831.t1 AT5G49240 52.727 55 26 0 85 139 218 272 1.12e-12 67.4
MS.gene58831.t1 AT1G25550 51.667 60 28 1 89 147 208 267 1.90e-12 67.4
MS.gene58831.t1 AT1G49190 32.692 104 62 4 89 186 214 315 9.37e-12 65.5
MS.gene58831.t1 AT2G01060 58.182 55 22 1 90 143 15 69 1.02e-11 64.7
MS.gene58831.t1 AT1G68670 50.000 62 30 1 87 147 212 273 1.69e-11 64.7
MS.gene58831.t1 AT1G49560 48.387 62 31 1 87 147 189 250 2.82e-11 63.5
MS.gene58831.t1 AT4G37180 50.000 60 29 1 89 147 209 268 3.17e-11 63.5
MS.gene58831.t1 AT4G37180 50.000 60 29 1 89 147 216 275 3.67e-11 63.5
MS.gene58831.t1 AT3G13040 53.571 56 25 1 89 143 240 295 4.39e-11 63.5
MS.gene58831.t1 AT3G13040 53.571 56 25 1 89 143 240 295 4.39e-11 63.5
MS.gene58831.t1 AT3G13040 53.571 56 25 1 89 143 240 295 4.39e-11 63.5

Find 101 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATAGTGGTGGGTGGATT+AGG 0.144441 7.3:-20416109 None:intergenic
CATCGGCCGGTTGGACATTT+CGG 0.221084 7.3:+20416628 MS.gene58831:CDS
CAGCAGCAGCTTCATAGAAT+TGG 0.261093 7.3:+20416736 MS.gene58831:CDS
GGATAGTGGTGGGTGGATTA+GGG 0.278062 7.3:-20416108 None:intergenic
TGAGAGAAGATGATTCAAAT+TGG 0.306698 7.3:+20415981 MS.gene58831:CDS
GGTTGTTGTTGTTGTGGTGG+TGG 0.318810 7.3:-20416130 None:intergenic
TGGCGAGATCAGGTGTGATT+AGG 0.327778 7.3:-20416063 None:intergenic
TTCAGTTGAATCGGCGAAAT+CGG 0.335433 7.3:-20416179 None:intergenic
TCCTGTTGCGCGGCCGAATT+CGG 0.336857 7.3:+20416537 MS.gene58831:CDS
TGGTGGTTATGTGGAGGATA+TGG 0.336921 7.3:+20416792 MS.gene58831:CDS
ATCGGCCGGTTGGACATTTC+GGG 0.337296 7.3:+20416629 MS.gene58831:CDS
AGCAGCAGCTTCATAGAATT+GGG 0.346797 7.3:+20416737 MS.gene58831:CDS
CGCATGCAGGGTATTTCTTC+CGG 0.354356 7.3:+20416418 MS.gene58831:CDS
GGCGAGATCAGGTGTGATTA+GGG 0.364114 7.3:-20416062 None:intergenic
GTGGAGGAAGGAAGAGAAGA+AGG 0.372281 7.3:-20416157 None:intergenic
GTTGTTGTTGTTGTGGTGGT+GGG 0.378316 7.3:-20416129 None:intergenic
GATAGAGGCATGAGTTCTTC+TGG 0.385240 7.3:-20416034 None:intergenic
CGGCGTCGACGAAGCGTTTG+TGG 0.387001 7.3:-20416276 None:intergenic
GTGATTAGGGTTTGTGATAG+AGG 0.393024 7.3:-20416049 None:intergenic
TTGTTGTGGTGGTGGGATAG+TGG 0.396749 7.3:-20416122 None:intergenic
CAATTCTATGAAGCTGCTGC+TGG 0.402280 7.3:-20416735 None:intergenic
GGTGGTGGTGGTTCCGGTGG+TGG 0.402979 7.3:+20416442 MS.gene58831:CDS
TCCGAATTCGGCCGCGCAAC+AGG 0.403622 7.3:-20416538 None:intergenic
TGGTTCTTCGCCGGCGGTGC+CGG 0.408437 7.3:-20416209 None:intergenic
TGAAGAAAATGCGGCGGTAC+AGG 0.411146 7.3:-20416514 None:intergenic
GGCATAAAATGATCCGAATT+CGG 0.417026 7.3:-20416550 None:intergenic
ACCGGAACCACCACCACCAC+CGG 0.421482 7.3:-20416437 None:intergenic
TATGAAGCTGCTGCTGGTGC+TGG 0.424890 7.3:-20416729 None:intergenic
TCGAAAGCGATGGCGAGATC+AGG 0.427672 7.3:-20416073 None:intergenic
TGGAGGAAGGAAGAGAAGAA+GGG 0.433177 7.3:-20416156 None:intergenic
CAGGTGCATCATAACACTTT+AGG 0.433744 7.3:+20416763 MS.gene58831:CDS
CATAAGCTGCATTATCGTCT+TGG 0.434900 7.3:-20416329 None:intergenic
ATTTCTTCCGGTGGTGGTGG+TGG 0.438743 7.3:+20416430 MS.gene58831:CDS
TTGTGGTGGTGGGATAGTGG+TGG 0.448450 7.3:-20416119 None:intergenic
TCAAACGCCCTCGTCTTGTA+TGG 0.450475 7.3:+20416242 MS.gene58831:CDS
TCTTTATCTTAAACGCATGC+AGG 0.456959 7.3:+20416405 MS.gene58831:CDS
GGCATGAGTTCTTCTGGTGG+TGG 0.457580 7.3:-20416028 None:intergenic
CAGGGTATTTCTTCCGGTGG+TGG 0.460767 7.3:+20416424 MS.gene58831:CDS
GGTATTTCTTCCGGTGGTGG+TGG 0.460989 7.3:+20416427 MS.gene58831:CDS
CGAAGCGTTTGTGGAGCTGT+GGG 0.467151 7.3:-20416267 None:intergenic
GTTCTGACTTTGTTTCCAAC+CGG 0.469745 7.3:+20416844 MS.gene58831:CDS
TGCTGCTCAGTATCATCGGC+CGG 0.482432 7.3:+20416615 MS.gene58831:CDS
GTCGACGCCGTGGCGCATCT+CGG 0.482986 7.3:+20416289 MS.gene58831:CDS
GGTGGTTGTTGTTGTTGTGG+TGG 0.484791 7.3:-20416133 None:intergenic
TGATGATGAATAGCAGGAAC+AGG 0.487494 7.3:-20416580 None:intergenic
ATGGAATCAGCTAATGCAAC+CGG 0.487862 7.3:+20416811 MS.gene58831:CDS
ATAAGCTGCATTATCGTCTT+GGG 0.488899 7.3:-20416328 None:intergenic
AACCCAAATTCAATCATCTC+CGG 0.488992 7.3:-20416863 None:intergenic
GGATTATCAAAATGTCCATT+CGG 0.494489 7.3:-20416658 None:intergenic
GAGGAAGGAAGAGAAGAAGG+GGG 0.497445 7.3:-20416154 None:intergenic
TAATGCAACCGGAGGGAGAA+AGG 0.508295 7.3:+20416822 MS.gene58831:CDS
GGAGGTGAGTTCAGTTGAAT+CGG 0.513901 7.3:-20416188 None:intergenic
AACACTTTAGGTGGTGGTGG+TGG 0.518249 7.3:+20416775 MS.gene58831:CDS
TGCTGTTGATGATGAATAGC+AGG 0.520424 7.3:-20416586 None:intergenic
GGCGACCCGAAATGTCCAAC+CGG 0.526706 7.3:-20416634 None:intergenic
GGAGGAAGGAAGAGAAGAAG+GGG 0.527059 7.3:-20416155 None:intergenic
AGTCAGAACCTTTCTCCCTC+CGG 0.527350 7.3:-20416830 None:intergenic
ACGAAGCGTTTGTGGAGCTG+TGG 0.532297 7.3:-20416268 None:intergenic
GCATGAGTTCTTCTGGTGGT+GGG 0.538620 7.3:-20416027 None:intergenic
TGAATCGGCGAAATCGGTGG+AGG 0.543830 7.3:-20416173 None:intergenic
TTCTTCGCCGGCGGTGCCGG+AGG 0.545793 7.3:-20416206 None:intergenic
GAACTCACCTCCGGCACCGC+CGG 0.547744 7.3:+20416199 MS.gene58831:CDS
TGTGGTGGTGGGATAGTGGT+GGG 0.548736 7.3:-20416118 None:intergenic
GCTTCATAGAATTGGGGCAC+AGG 0.550758 7.3:+20416744 MS.gene58831:CDS
CATAACACTTTAGGTGGTGG+TGG 0.551977 7.3:+20416772 MS.gene58831:CDS
GCTCAGTATCATCGGCCGGT+TGG 0.552483 7.3:+20416619 MS.gene58831:CDS
AGTTGAATCGGCGAAATCGG+TGG 0.557119 7.3:-20416176 None:intergenic
GAAGAAAATGCGGCGGTACA+GGG 0.558776 7.3:-20416513 None:intergenic
GGGCGTTTGAGTGTGCGAGC+TGG 0.560084 7.3:-20416229 None:intergenic
CTTTATCTTAAACGCATGCA+GGG 0.564649 7.3:+20416406 MS.gene58831:CDS
CATTTCGGGTCGCCGCCGAA+TGG 0.568846 7.3:+20416643 MS.gene58831:CDS
TGTGCGAGCTGGTTCTTCGC+CGG 0.569228 7.3:-20416218 None:intergenic
TCGGCGAAATCGGTGGAGGA+AGG 0.569986 7.3:-20416169 None:intergenic
ATAGCAGGAACAGGAACAAA+CGG 0.570737 7.3:-20416571 None:intergenic
CATCATAACACTTTAGGTGG+TGG 0.574928 7.3:+20416769 MS.gene58831:CDS
AACAGAAGCAGAACCACCAC+CGG 0.574953 7.3:-20416455 None:intergenic
GTTTCTTATGTCGAAAGCGA+TGG 0.581395 7.3:-20416083 None:intergenic
AAGAAAATGCGGCGGTACAG+GGG 0.587168 7.3:-20416512 None:intergenic
ATGCAGGGTATTTCTTCCGG+TGG 0.591915 7.3:+20416421 MS.gene58831:CDS
CAAATTCAATCATCTCCGGT+TGG 0.598529 7.3:-20416859 None:intergenic
GGTGGTGGGATAGTGGTGGG+TGG 0.599530 7.3:-20416115 None:intergenic
ATGCAGCTTATGAGTGTCGA+CGG 0.600961 7.3:+20416340 MS.gene58831:CDS
GCGAGCTGGTTCTTCGCCGG+CGG 0.605171 7.3:-20416215 None:intergenic
TATTCATCATCAACAGCAGA+TGG 0.608957 7.3:+20416591 MS.gene58831:CDS
TGGCTGCTGCTCAGTATCAT+CGG 0.613909 7.3:+20416611 MS.gene58831:CDS
CGGTTTAACGAGAGAAAACG+TGG 0.626632 7.3:+20416360 MS.gene58831:CDS
CTTGATACCGAGATGCGCCA+CGG 0.632900 7.3:-20416296 None:intergenic
GAATCAGCTAATGCAACCGG+AGG 0.639788 7.3:+20416814 MS.gene58831:CDS
GATTCAACTGAACTCACCTC+CGG 0.640353 7.3:+20416190 MS.gene58831:CDS
TGGTGGTGGTGGTTATGTGG+AGG 0.660011 7.3:+20416786 MS.gene58831:CDS
GCAGCAGCTTCATAGAATTG+GGG 0.666624 7.3:+20416738 MS.gene58831:CDS
GTGCATCATAACACTTTAGG+TGG 0.672948 7.3:+20416766 MS.gene58831:CDS
TGTGGGGTCCATACAAGACG+AGG 0.673250 7.3:-20416250 None:intergenic
CAAACGCTTCGTCGACGCCG+TGG 0.674680 7.3:+20416279 MS.gene58831:CDS
GCAACAGGATGAAGAAAATG+CGG 0.684941 7.3:-20416523 None:intergenic
GAAGCGTTTGTGGAGCTGTG+GGG 0.695777 7.3:-20416266 None:intergenic
TTATCAAAATGTCCATTCGG+CGG 0.700387 7.3:-20416655 None:intergenic
AATCAGCTAATGCAACCGGA+GGG 0.707357 7.3:+20416815 MS.gene58831:CDS
GTGGGGTCCATACAAGACGA+GGG 0.708697 7.3:-20416249 None:intergenic
AGAGGCATGAGTTCTTCTGG+TGG 0.719854 7.3:-20416031 None:intergenic
ACAGGATGAAGAAAATGCGG+CGG 0.721657 7.3:-20416520 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAAATTGGTTTTCAAGA+TGG + chr7.3:20415993-20416012 MS.gene58831:CDS 25.0%
!!! TTCAAATTGGTTTTCAAGAT+GGG + chr7.3:20415994-20416013 MS.gene58831:CDS 25.0%
GGATTATCAAAATGTCCATT+CGG - chr7.3:20416661-20416680 None:intergenic 30.0%
TGAGAGAAGATGATTCAAAT+TGG + chr7.3:20415981-20416000 MS.gene58831:CDS 30.0%
ATAAGCTGCATTATCGTCTT+GGG - chr7.3:20416331-20416350 None:intergenic 35.0%
CTTTATCTTAAACGCATGCA+GGG + chr7.3:20416406-20416425 MS.gene58831:CDS 35.0%
GGCATAAAATGATCCGAATT+CGG - chr7.3:20416553-20416572 None:intergenic 35.0%
TATTCATCATCAACAGCAGA+TGG + chr7.3:20416591-20416610 MS.gene58831:CDS 35.0%
TCTTTATCTTAAACGCATGC+AGG + chr7.3:20416405-20416424 MS.gene58831:CDS 35.0%
TGTTGCTGTCTAGACAAAAA+AGG - chr7.3:20416682-20416701 None:intergenic 35.0%
TTATCAAAATGTCCATTCGG+CGG - chr7.3:20416658-20416677 None:intergenic 35.0%
AGCAGCAGCTTCATAGAATT+GGG + chr7.3:20416737-20416756 MS.gene58831:CDS 40.0%
ATAGCAGGAACAGGAACAAA+CGG - chr7.3:20416574-20416593 None:intergenic 40.0%
ATGGAATCAGCTAATGCAAC+CGG + chr7.3:20416811-20416830 MS.gene58831:CDS 40.0%
CAGGTGCATCATAACACTTT+AGG + chr7.3:20416763-20416782 MS.gene58831:CDS 40.0%
CATAAGCTGCATTATCGTCT+TGG - chr7.3:20416332-20416351 None:intergenic 40.0%
CATCATAACACTTTAGGTGG+TGG + chr7.3:20416769-20416788 MS.gene58831:CDS 40.0%
GCAACAGGATGAAGAAAATG+CGG - chr7.3:20416526-20416545 None:intergenic 40.0%
GTGATTAGGGTTTGTGATAG+AGG - chr7.3:20416052-20416071 None:intergenic 40.0%
GTGCATCATAACACTTTAGG+TGG + chr7.3:20416766-20416785 MS.gene58831:CDS 40.0%
GTTTCTTATGTCGAAAGCGA+TGG - chr7.3:20416086-20416105 None:intergenic 40.0%
TTCAGTTGAATCGGCGAAAT+CGG - chr7.3:20416182-20416201 None:intergenic 40.0%
! ATTTTCTTCATCCTGTTGCG+CGG + chr7.3:20416527-20416546 MS.gene58831:CDS 40.0%
! GTTCTGACTTTGTTTCCAAC+CGG + chr7.3:20416844-20416863 MS.gene58831:CDS 40.0%
! TGATGATGAATAGCAGGAAC+AGG - chr7.3:20416583-20416602 None:intergenic 40.0%
!! TGCTGTTGATGATGAATAGC+AGG - chr7.3:20416589-20416608 None:intergenic 40.0%
AATCAGCTAATGCAACCGGA+GGG + chr7.3:20416815-20416834 MS.gene58831:CDS 45.0%
ACAGGATGAAGAAAATGCGG+CGG - chr7.3:20416523-20416542 None:intergenic 45.0%
CAATTCTATGAAGCTGCTGC+TGG - chr7.3:20416738-20416757 None:intergenic 45.0%
CAGCAGCAGCTTCATAGAAT+TGG + chr7.3:20416736-20416755 MS.gene58831:CDS 45.0%
CGGTTTAACGAGAGAAAACG+TGG + chr7.3:20416360-20416379 MS.gene58831:CDS 45.0%
GATTCAACTGAACTCACCTC+CGG + chr7.3:20416190-20416209 MS.gene58831:CDS 45.0%
GCAGCAGCTTCATAGAATTG+GGG + chr7.3:20416738-20416757 MS.gene58831:CDS 45.0%
GGAGGTGAGTTCAGTTGAAT+CGG - chr7.3:20416191-20416210 None:intergenic 45.0%
TGGAGGAAGGAAGAGAAGAA+GGG - chr7.3:20416159-20416178 None:intergenic 45.0%
TGGTGGTTATGTGGAGGATA+TGG + chr7.3:20416792-20416811 MS.gene58831:CDS 45.0%
! CATAACACTTTAGGTGGTGG+TGG + chr7.3:20416772-20416791 MS.gene58831:CDS 45.0%
! GATAGAGGCATGAGTTCTTC+TGG - chr7.3:20416037-20416056 None:intergenic 45.0%
! GTTGTTGTTGTTGTGGTGGT+GGG - chr7.3:20416132-20416151 None:intergenic 45.0%
!! ATGCAGCTTATGAGTGTCGA+CGG + chr7.3:20416340-20416359 MS.gene58831:CDS 45.0%
AACAGAAGCAGAACCACCAC+CGG - chr7.3:20416458-20416477 None:intergenic 50.0%
AAGAAAATGCGGCGGTACAG+GGG - chr7.3:20416515-20416534 None:intergenic 50.0%
AGTCAGAACCTTTCTCCCTC+CGG - chr7.3:20416833-20416852 None:intergenic 50.0%
AGTTGAATCGGCGAAATCGG+TGG - chr7.3:20416179-20416198 None:intergenic 50.0%
GAAGAAAATGCGGCGGTACA+GGG - chr7.3:20416516-20416535 None:intergenic 50.0%
GAATCAGCTAATGCAACCGG+AGG + chr7.3:20416814-20416833 MS.gene58831:CDS 50.0%
GAGGAAGGAAGAGAAGAAGG+GGG - chr7.3:20416157-20416176 None:intergenic 50.0%
GCATGAGTTCTTCTGGTGGT+GGG - chr7.3:20416030-20416049 None:intergenic 50.0%
GCTTCATAGAATTGGGGCAC+AGG + chr7.3:20416744-20416763 MS.gene58831:CDS 50.0%
GGAGGAAGGAAGAGAAGAAG+GGG - chr7.3:20416158-20416177 None:intergenic 50.0%
GGCGAGATCAGGTGTGATTA+GGG - chr7.3:20416065-20416084 None:intergenic 50.0%
GTGGAGGAAGGAAGAGAAGA+AGG - chr7.3:20416160-20416179 None:intergenic 50.0%
TAATGCAACCGGAGGGAGAA+AGG + chr7.3:20416822-20416841 MS.gene58831:CDS 50.0%
TCAAACGCCCTCGTCTTGTA+TGG + chr7.3:20416242-20416261 MS.gene58831:CDS 50.0%
TGAAGAAAATGCGGCGGTAC+AGG - chr7.3:20416517-20416536 None:intergenic 50.0%
TGGCGAGATCAGGTGTGATT+AGG - chr7.3:20416066-20416085 None:intergenic 50.0%
TGGCTGCTGCTCAGTATCAT+CGG + chr7.3:20416611-20416630 MS.gene58831:CDS 50.0%
! AGAGGCATGAGTTCTTCTGG+TGG - chr7.3:20416034-20416053 None:intergenic 50.0%
! ATGCAGGGTATTTCTTCCGG+TGG + chr7.3:20416421-20416440 MS.gene58831:CDS 50.0%
! CGCATGCAGGGTATTTCTTC+CGG + chr7.3:20416418-20416437 MS.gene58831:CDS 50.0%
! GGTGGTTGTTGTTGTTGTGG+TGG - chr7.3:20416136-20416155 None:intergenic 50.0%
! GGTTGTTGTTGTTGTGGTGG+TGG - chr7.3:20416133-20416152 None:intergenic 50.0%
!! AACACTTTAGGTGGTGGTGG+TGG + chr7.3:20416775-20416794 MS.gene58831:CDS 50.0%
!! GGATAGTGGTGGGTGGATTA+GGG - chr7.3:20416111-20416130 None:intergenic 50.0%
!! TTGTTGTGGTGGTGGGATAG+TGG - chr7.3:20416125-20416144 None:intergenic 50.0%
ATCGGCCGGTTGGACATTTC+GGG + chr7.3:20416629-20416648 MS.gene58831:CDS 55.0%
CATCGGCCGGTTGGACATTT+CGG + chr7.3:20416628-20416647 MS.gene58831:CDS 55.0%
CTTGATACCGAGATGCGCCA+CGG - chr7.3:20416299-20416318 None:intergenic 55.0%
GAAGGAAGAGAAGAAGGGGG+TGG - chr7.3:20416154-20416173 None:intergenic 55.0%
GGCATGAGTTCTTCTGGTGG+TGG - chr7.3:20416031-20416050 None:intergenic 55.0%
GTGGGGTCCATACAAGACGA+GGG - chr7.3:20416252-20416271 None:intergenic 55.0%
TATGAAGCTGCTGCTGGTGC+TGG - chr7.3:20416732-20416751 None:intergenic 55.0%
TCGAAAGCGATGGCGAGATC+AGG - chr7.3:20416076-20416095 None:intergenic 55.0%
TGAATCGGCGAAATCGGTGG+AGG - chr7.3:20416176-20416195 None:intergenic 55.0%
TGCTGCTCAGTATCATCGGC+CGG + chr7.3:20416615-20416634 MS.gene58831:CDS 55.0%
TGTGGGGTCCATACAAGACG+AGG - chr7.3:20416253-20416272 None:intergenic 55.0%
! ACGAAGCGTTTGTGGAGCTG+TGG - chr7.3:20416271-20416290 None:intergenic 55.0%
! CAGGGTATTTCTTCCGGTGG+TGG + chr7.3:20416424-20416443 MS.gene58831:CDS 55.0%
! CGAAGCGTTTGTGGAGCTGT+GGG - chr7.3:20416270-20416289 None:intergenic 55.0%
! GAAGCGTTTGTGGAGCTGTG+GGG - chr7.3:20416269-20416288 None:intergenic 55.0%
! GGGGGTGGTTGTTGTTGTTG+TGG - chr7.3:20416139-20416158 None:intergenic 55.0%
! GGTATTTCTTCCGGTGGTGG+TGG + chr7.3:20416427-20416446 MS.gene58831:CDS 55.0%
!! AGGTGGTGGTGGTGGTTATG+TGG + chr7.3:20416783-20416802 MS.gene58831:CDS 55.0%
!! ATTTCTTCCGGTGGTGGTGG+TGG + chr7.3:20416430-20416449 MS.gene58831:CDS 55.0%
!! GGGATAGTGGTGGGTGGATT+AGG - chr7.3:20416112-20416131 None:intergenic 55.0%
!! TGGTGGTGGTGGTTATGTGG+AGG + chr7.3:20416786-20416805 MS.gene58831:CDS 55.0%
!! TGTGGTGGTGGGATAGTGGT+GGG - chr7.3:20416121-20416140 None:intergenic 55.0%
!! TTGTGGTGGTGGGATAGTGG+TGG - chr7.3:20416122-20416141 None:intergenic 55.0%
GCTCAGTATCATCGGCCGGT+TGG + chr7.3:20416619-20416638 MS.gene58831:CDS 60.0%
GGCGACCCGAAATGTCCAAC+CGG - chr7.3:20416637-20416656 None:intergenic 60.0%
TCCTGTTGCGCGGCCGAATT+CGG + chr7.3:20416537-20416556 MS.gene58831:CDS 60.0%
TCGGCGAAATCGGTGGAGGA+AGG - chr7.3:20416172-20416191 None:intergenic 60.0%
! TGTGCGAGCTGGTTCTTCGC+CGG - chr7.3:20416221-20416240 None:intergenic 60.0%
ACCGGAACCACCACCACCAC+CGG - chr7.3:20416440-20416459 None:intergenic 65.0%
CAAACGCTTCGTCGACGCCG+TGG + chr7.3:20416279-20416298 MS.gene58831:CDS 65.0%
GGGCGTTTGAGTGTGCGAGC+TGG - chr7.3:20416232-20416251 None:intergenic 65.0%
TCCGAATTCGGCCGCGCAAC+AGG - chr7.3:20416541-20416560 None:intergenic 65.0%
! CATTTCGGGTCGCCGCCGAA+TGG + chr7.3:20416643-20416662 MS.gene58831:CDS 65.0%
!! CGGCGTCGACGAAGCGTTTG+TGG - chr7.3:20416279-20416298 None:intergenic 65.0%
!! GGTGGTGGGATAGTGGTGGG+TGG - chr7.3:20416118-20416137 None:intergenic 65.0%
!! TCCGGTGGTGGTGGTGGTTC+CGG + chr7.3:20416436-20416455 MS.gene58831:CDS 65.0%
GAACTCACCTCCGGCACCGC+CGG + chr7.3:20416199-20416218 MS.gene58831:CDS 70.0%
GTCGACGCCGTGGCGCATCT+CGG + chr7.3:20416289-20416308 MS.gene58831:CDS 70.0%
TGGTTCTTCGCCGGCGGTGC+CGG - chr7.3:20416212-20416231 None:intergenic 70.0%
! GCGAGCTGGTTCTTCGCCGG+CGG - chr7.3:20416218-20416237 None:intergenic 70.0%
!! GGTGGTGGTGGTGGTTCCGG+TGG + chr7.3:20416439-20416458 MS.gene58831:CDS 70.0%
!! GGTGGTGGTGGTTCCGGTGG+TGG + chr7.3:20416442-20416461 MS.gene58831:CDS 70.0%
! TTCTTCGCCGGCGGTGCCGG+AGG - chr7.3:20416209-20416228 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 20415980 20416876 20415980 ID=MS.gene58831
chr7.3 mRNA 20415980 20416876 20415980 ID=MS.gene58831.t1;Parent=MS.gene58831
chr7.3 exon 20415980 20416876 20415980 ID=MS.gene58831.t1.exon1;Parent=MS.gene58831.t1
chr7.3 CDS 20415980 20416876 20415980 ID=cds.MS.gene58831.t1;Parent=MS.gene58831.t1
Gene Sequence

>MS.gene58831

ATGAGAGAAGATGATTCAAATTGGTTTTCAAGATGGGAAGAACAACTCCCACCACCAGAAGAACTCATGCCTCTATCACAAACCCTAATCACACCTGATCTCGCCATCGCTTTCGACATAAGAAACAACCCTAATCCACCCACCACTATCCCACCACCACAACAACAACAACCACCCCCTTCTTCTCTTCCTTCCTCCACCGATTTCGCCGATTCAACTGAACTCACCTCCGGCACCGCCGGCGAAGAACCAGCTCGCACACTCAAACGCCCTCGTCTTGTATGGACCCCACAGCTCCACAAACGCTTCGTCGACGCCGTGGCGCATCTCGGTATCAAGAACGCTGTTCCCAAGACGATAATGCAGCTTATGAGTGTCGACGGTTTAACGAGAGAAAACGTGGCGAGTCATCTTCAGAAATATCGTCTTTATCTTAAACGCATGCAGGGTATTTCTTCCGGTGGTGGTGGTGGTTCCGGTGGTGGTTCTGCTTCTGTTAATGATGCTGCTACTGATCATTTATTTGCTAGCTCCCCTGTACCGCCGCATTTTCTTCATCCTGTTGCGCGGCCGAATTCGGATCATTTTATGCCGTTTGTTCCTGTTCCTGCTATTCATCATCAACAGCAGATGGCTGCTGCTCAGTATCATCGGCCGGTTGGACATTTCGGGTCGCCGCCGAATGGACATTTTGATAATCCTTTTTTGTCTAGACAGCAACATCATCAACAGCAAAATCAGCATCAACACCAGCACCAGCAGCAGCTTCATAGAATTGGGGCACAGGTGCATCATAACACTTTAGGTGGTGGTGGTGGTTATGTGGAGGATATGGAATCAGCTAATGCAACCGGAGGGAGAAAGGTTCTGACTTTGTTTCCAACCGGAGATGATTGA
Protein sequence

>MS.gene58831.t1

MREDDSNWFSRWEEQLPPPEELMPLSQTLITPDLAIAFDIRNNPNPPTTIPPPQQQQPPPSSLPSSTDFADSTELTSGTAGEEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGISSGGGGGSGGGSASVNDAATDHLFASSPVPPHFLHPVARPNSDHFMPFVPVPAIHHQQQMAAAQYHRPVGHFGSPPNGHFDNPFLSRQQHHQQQNQHQHQHQQQLHRIGAQVHHNTLGGGGGYVEDMESANATGGRKVLTLFPTGDD