Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58846.t1 | XP_003607052.1 | 77.9 | 149 | 33 | 0 | 1 | 149 | 1 | 149 | 1.70E-56 | 228.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58846.t1 | F4JZT3 | 32.4 | 105 | 71 | 0 | 33 | 137 | 18 | 122 | 9.8e-13 | 74.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58846.t1 | G7JJU6 | 77.9 | 149 | 33 | 0 | 1 | 149 | 1 | 149 | 1.2e-56 | 228.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene58846.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58846.t1 | MTR_6g008860 | 77.852 | 149 | 33 | 0 | 1 | 149 | 1 | 149 | 1.08e-82 | 240 |
| MS.gene58846.t1 | MTR_4g071650 | 77.852 | 149 | 33 | 0 | 1 | 149 | 1 | 149 | 1.08e-82 | 240 |
| MS.gene58846.t1 | MTR_7g089310 | 93.182 | 44 | 3 | 0 | 102 | 145 | 1 | 44 | 1.43e-24 | 90.9 |
| MS.gene58846.t1 | MTR_7g099500 | 30.702 | 114 | 79 | 0 | 33 | 146 | 11 | 124 | 3.30e-16 | 70.9 |
| MS.gene58846.t1 | MTR_8g063580 | 31.304 | 115 | 77 | 1 | 33 | 145 | 12 | 126 | 1.17e-14 | 67.0 |
| MS.gene58846.t1 | MTR_7g098910 | 30.973 | 113 | 77 | 1 | 25 | 137 | 14 | 125 | 3.81e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58846.t1 | AT5G26594 | 32.381 | 105 | 71 | 0 | 33 | 137 | 18 | 122 | 1.39e-16 | 72.0 |
Find 0 sgRNAs with CRISPR-Local
Find 75 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTTAATAATCCTCAT+GGG | - | 50879:31846-31865 | MS.gene58846:intron | 15.0% |
| !! | TAATAATTTAATAATCCTCA+TGG | - | 50879:31845-31864 | MS.gene58846:intron | 15.0% |
| !! | AAAAGTTAACGTCATATTTA+CGG | - | 50879:31477-31496 | MS.gene58846:intron | 20.0% |
| !! | ATAAAGAAATTGTTTATACC+TGG | + | 50879:31381-31400 | None:intergenic | 20.0% |
| !! | TAAAGAAATTGTTTATACCT+GGG | + | 50879:31380-31399 | None:intergenic | 20.0% |
| !! | TTATGAACATTACATGAAAT+GGG | + | 50879:32097-32116 | None:intergenic | 20.0% |
| ! | AAACTTAATGCTTATCAACT+AGG | - | 50879:31632-31651 | MS.gene58846:intron | 25.0% |
| ! | AAAGAAATTGTTTATACCTG+GGG | + | 50879:31379-31398 | None:intergenic | 25.0% |
| ! | ACTACCACTAAAATAAATTG+TGG | - | 50879:32146-32165 | MS.gene58846:CDS | 25.0% |
| ! | ATAATCTGATTGTCTAGATT+CGG | + | 50879:31735-31754 | None:intergenic | 25.0% |
| ! | CTCTTTCATTTGTTTATATC+TGG | + | 50879:31119-31138 | None:intergenic | 25.0% |
| ! | GTCTTTGGTTAAAATCATTT+AGG | - | 50879:31917-31936 | MS.gene58846:intron | 25.0% |
| ! | GTTATGAACATTACATGAAA+TGG | + | 50879:32098-32117 | None:intergenic | 25.0% |
| ! | TCAAAACTTTCTTTGGTTAA+AGG | + | 50879:32307-32326 | None:intergenic | 25.0% |
| ! | TTTCATTTGTTTATATCTGG+TGG | + | 50879:31116-31135 | None:intergenic | 25.0% |
| !!! | ACAACCACAATTTATTTTAG+TGG | + | 50879:32153-32172 | None:intergenic | 25.0% |
| !!! | TAGTTGATAAGCATTAAGTT+TGG | + | 50879:31633-31652 | None:intergenic | 25.0% |
| !!! | TTTGATTTGTCCATAATCAA+TGG | - | 50879:31352-31371 | MS.gene58846:intron | 25.0% |
| ATCAATGCATTGCTAATAGA+TGG | - | 50879:31196-31215 | MS.gene58846:CDS | 30.0% | |
| CATCAAATGCTTGAATCAAT+AGG | - | 50879:31244-31263 | MS.gene58846:CDS | 30.0% | |
| CATTAAGTTTGGATCTAGAT+AGG | + | 50879:31622-31641 | None:intergenic | 30.0% | |
| CCTACAATCTAACTAACTTA+AGG | + | 50879:32030-32049 | None:intergenic | 30.0% | |
| CCTTAAGTTAGTTAGATTGT+AGG | - | 50879:32027-32046 | MS.gene58846:intron | 30.0% | |
| TAGCACCAGTCAATAAAATA+TGG | + | 50879:31309-31328 | None:intergenic | 30.0% | |
| TTTCGCTCATGTATGATTAT+AGG | + | 50879:31681-31700 | None:intergenic | 30.0% | |
| ! | ATTCTCATTTTCTTCTCATC+AGG | + | 50879:31142-31161 | None:intergenic | 30.0% |
| ! | CTTTCTTTGGTTAAAGGTTT+TGG | + | 50879:32301-32320 | None:intergenic | 30.0% |
| ! | GCAATCCATATTTTATTGAC+TGG | - | 50879:31301-31320 | MS.gene58846:intron | 30.0% |
| ! | GTGGTTGTATTTTATGTAGT+TGG | - | 50879:32165-32184 | MS.gene58846:CDS | 30.0% |
| ! | TCTCTCTTTTAATCTAAGTC+TGG | - | 50879:31769-31788 | MS.gene58846:intron | 30.0% |
| !! | AAAATGAGTTGATAGCTACA+TGG | - | 50879:31442-31461 | MS.gene58846:intron | 30.0% |
| !! | TTTTAAGCGAACTTTGAGTT+GGG | + | 50879:31535-31554 | None:intergenic | 30.0% |
| !! | TTTTTAAGCGAACTTTGAGT+TGG | + | 50879:31536-31555 | None:intergenic | 30.0% |
| !!! | AACCAAAGAAAGTTTTGAAC+TGG | - | 50879:32309-32328 | MS.gene58846:CDS | 30.0% |
| !!! | GCTTTTTTTGTTGCTTCAAT+TGG | - | 50879:31976-31995 | MS.gene58846:intron | 30.0% |
| AGTTAACGTCATATTTACGG+TGG | - | 50879:31480-31499 | MS.gene58846:intron | 35.0% | |
| CCAATCTCATCATCAATTAG+CGG | + | 50879:32006-32025 | None:intergenic | 35.0% | |
| GACCAGTTCAAAACTTTCTT+TGG | + | 50879:32314-32333 | None:intergenic | 35.0% | |
| TAGAAACATCCTCGTATTGA+CGG | - | 50879:31578-31597 | MS.gene58846:intron | 35.0% | |
| TCATATTTACGGTGGATCTT+GGG | - | 50879:31488-31507 | MS.gene58846:intron | 35.0% | |
| ! | AGCAATGCATTGATGAGATA+AGG | + | 50879:31190-31209 | None:intergenic | 35.0% |
| ! | CAATACGAGGATGTTTCTAA+TGG | + | 50879:31577-31596 | None:intergenic | 35.0% |
| ! | TTTTAACCAAAGACCTAGAG+AGG | + | 50879:31911-31930 | None:intergenic | 35.0% |
| !! | TTTAAGCGAACTTTGAGTTG+GGG | + | 50879:31534-31553 | None:intergenic | 35.0% |
| !!! | GAAAGTTTTGAACTGGTCAT+TGG | - | 50879:32316-32335 | MS.gene58846:CDS | 35.0% |
| AATGGGGATACAAAGCAAGA+TGG | - | 50879:32204-32223 | MS.gene58846:CDS | 40.0% | |
| ATGTCCACCACATGAATTGT+AGG | + | 50879:31945-31964 | None:intergenic | 40.0% | |
| CATATTTACGGTGGATCTTG+GGG | - | 50879:31489-31508 | MS.gene58846:intron | 40.0% | |
| CTAGAGAGGAGTAAATACCT+AGG | + | 50879:31897-31916 | None:intergenic | 40.0% | |
| CTAGGTATTTACTCCTCTCT+AGG | - | 50879:31895-31914 | MS.gene58846:intron | 40.0% | |
| CTTCATAAAGTCGACGACAA+AGG | + | 50879:31709-31728 | None:intergenic | 40.0% | |
| GCTTCTTGAGAACTATTTGC+AGG | + | 50879:31283-31302 | None:intergenic | 40.0% | |
| GTCATATTTACGGTGGATCT+TGG | - | 50879:31487-31506 | MS.gene58846:intron | 40.0% | |
| TTAGGTGCCTACAATTCATG+TGG | - | 50879:31935-31954 | MS.gene58846:intron | 40.0% | |
| TTTACTCCTCTCTAGGTCTT+TGG | - | 50879:31902-31921 | MS.gene58846:intron | 40.0% | |
| ! | TGATCTGATGGTGTAGACTT+GGG | - | 50879:31870-31889 | MS.gene58846:intron | 40.0% |
| ! | TTGATCTGATGGTGTAGACT+TGG | - | 50879:31869-31888 | MS.gene58846:intron | 40.0% |
| !! | CCGCTAATTGATGATGAGAT+TGG | - | 50879:32003-32022 | MS.gene58846:intron | 40.0% |
| !! | TCAACTAGGCTGATTTTCGT+TGG | - | 50879:31646-31665 | MS.gene58846:intron | 40.0% |
| !!! | GGTTAAAGGTTTTGGAACAC+TGG | + | 50879:32293-32312 | None:intergenic | 40.0% |
| GGGATACAAAGCAAGATGGT+CGG | - | 50879:32208-32227 | MS.gene58846:CDS | 45.0% | |
| ! | AAACATCCTCGTATTGACGG+TGG | - | 50879:31581-31600 | MS.gene58846:intron | 45.0% |
| ! | AACATCCTCGTATTGACGGT+GGG | - | 50879:31582-31601 | MS.gene58846:intron | 45.0% |
| ! | TACCTGGGGTCCATTGATTA+TGG | + | 50879:31365-31384 | None:intergenic | 45.0% |
| !! | ATCCTCATGGGTTGATCTGA+TGG | - | 50879:31858-31877 | MS.gene58846:intron | 45.0% |
| !! | GTGGTGGACATGTTCATACA+AGG | - | 50879:31954-31973 | MS.gene58846:intron | 45.0% |
| ATGGTGTAGACTTGGGACCT+AGG | - | 50879:31877-31896 | MS.gene58846:intron | 50.0% | |
| CAAAACCCACCGTCAATACG+AGG | + | 50879:31590-31609 | None:intergenic | 50.0% | |
| CACCATCAGATCAACCCATG+AGG | + | 50879:31863-31882 | None:intergenic | 50.0% | |
| GGTGCCTACAATTCATGTGG+TGG | - | 50879:31938-31957 | MS.gene58846:intron | 50.0% | |
| GTCCATAATCAATGGACCCC+AGG | - | 50879:31360-31379 | MS.gene58846:intron | 50.0% | |
| GTGAGACAGATGCGTGCAAT+GGG | - | 50879:32187-32206 | MS.gene58846:CDS | 50.0% | |
| TGAGACAGATGCGTGCAATG+GGG | - | 50879:32188-32207 | MS.gene58846:CDS | 50.0% | |
| GGTGAGACAGATGCGTGCAA+TGG | - | 50879:32186-32205 | MS.gene58846:CDS | 55.0% | |
| TGAGACTGCAACGAGACGCT+TGG | - | 50879:31219-31238 | MS.gene58846:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 50879 | gene | 31100 | 32357 | 31100 | ID=MS.gene58846 |
| 50879 | mRNA | 31100 | 32357 | 31100 | ID=MS.gene58846.t1;Parent=MS.gene58846 |
| 50879 | exon | 32076 | 32357 | 32076 | ID=MS.gene58846.t1.exon1;Parent=MS.gene58846.t1 |
| 50879 | CDS | 32076 | 32357 | 32076 | ID=cds.MS.gene58846.t1;Parent=MS.gene58846.t1 |
| 50879 | exon | 31100 | 31273 | 31100 | ID=MS.gene58846.t1.exon2;Parent=MS.gene58846.t1 |
| 50879 | CDS | 31100 | 31273 | 31100 | ID=cds.MS.gene58846.t1;Parent=MS.gene58846.t1 |
| Gene Sequence |
| Protein sequence |
