Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58943.t1 | XP_003609586.1 | 91.7 | 181 | 15 | 0 | 1 | 181 | 1 | 181 | 3.80E-79 | 304.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58943.t1 | G7JJ33 | 91.7 | 181 | 15 | 0 | 1 | 181 | 1 | 181 | 2.7e-79 | 304.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene58943.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58943.t1 | MTR_4g118830 | 91.713 | 181 | 15 | 0 | 1 | 181 | 1 | 181 | 1.54e-117 | 330 |
| MS.gene58943.t1 | MTR_4g118430 | 45.946 | 111 | 41 | 3 | 77 | 181 | 1 | 98 | 3.68e-21 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTTCTCTGTTACGGTTAC+TGG | 0.183266 | 8.3:-7862018 | MS.gene58943:CDS |
| AGTAACCGTAACAGAGAAAT+TGG | 0.195649 | 8.3:+7862020 | None:intergenic |
| TCAAGCAATGTGGCTTTCTT+TGG | 0.199938 | 8.3:-7861994 | MS.gene58943:CDS |
| TGTTGGTTCCGTGTCGTAAT+AGG | 0.277898 | 8.3:-7862047 | MS.gene58943:CDS |
| GAGAGAGATTATGAGTTTGT+AGG | 0.295578 | 8.3:-7861809 | None:intergenic |
| CAAGCAATGTGGCTTTCTTT+GGG | 0.307847 | 8.3:-7861993 | MS.gene58943:CDS |
| ATCACTGCTTTGGTTCAAAA+TGG | 0.315294 | 8.3:-7861853 | MS.gene58943:CDS |
| TAACAAATCAGTTTCTGAAA+TGG | 0.363545 | 8.3:-7862457 | MS.gene58943:CDS |
| GATCTTGGCGATCACTGCTT+TGG | 0.383378 | 8.3:-7861863 | MS.gene58943:CDS |
| AACAAATCAGTTTCTGAAAT+GGG | 0.410347 | 8.3:-7862456 | MS.gene58943:CDS |
| TTACATTGGGGTAGAGAGTT+GGG | 0.421719 | 8.3:+7862328 | None:intergenic |
| GAATCCCAATTTCTCTGTTA+CGG | 0.433893 | 8.3:-7862025 | MS.gene58943:CDS |
| TAATGATGGTAATTTGATCT+TGG | 0.443216 | 8.3:-7861878 | MS.gene58943:CDS |
| GAAAATGTTGGAGGAGATGA+AGG | 0.445802 | 8.3:-7862109 | MS.gene58943:CDS |
| TGAAGAGAAGAAGAAAATGT+TGG | 0.449297 | 8.3:-7862121 | MS.gene58943:CDS |
| GTTACATTGGGGTAGAGAGT+TGG | 0.468914 | 8.3:+7862327 | None:intergenic |
| ATCAAAGAAACCTGTTGAAG+GGG | 0.470795 | 8.3:-7862079 | MS.gene58943:CDS |
| GCTAATGTTTCTGTTAATGA+TGG | 0.496957 | 8.3:-7861892 | MS.gene58943:CDS |
| TTCAACGTTTAGAACCTCTT+GGG | 0.500668 | 8.3:+7862404 | None:intergenic |
| TTTCTTTGGGATTGAATCTG+TGG | 0.501659 | 8.3:-7861980 | MS.gene58943:CDS |
| GAGTTGGGACAGTGGTAGTG+AGG | 0.504434 | 8.3:+7862343 | None:intergenic |
| GTTCAACGTTTAGAACCTCT+TGG | 0.509820 | 8.3:+7862403 | None:intergenic |
| ATTGAATCTGTGGCTAAACC+TGG | 0.515198 | 8.3:-7861970 | MS.gene58943:CDS |
| AAATCAAAGAAACCTGTTGA+AGG | 0.526426 | 8.3:-7862081 | MS.gene58943:CDS |
| AGAGAAGAAGAAAATGTTGG+AGG | 0.534106 | 8.3:-7862118 | MS.gene58943:CDS |
| AATCAAAGAAACCTGTTGAA+GGG | 0.548949 | 8.3:-7862080 | MS.gene58943:CDS |
| GTAACCGTAACAGAGAAATT+GGG | 0.551700 | 8.3:+7862021 | None:intergenic |
| TAAACGTTGAACGTGAAAGA+AGG | 0.567677 | 8.3:-7862392 | MS.gene58943:CDS |
| TCAAAGAAACCTGTTGAAGG+GGG | 0.622647 | 8.3:-7862078 | MS.gene58943:CDS |
| AAAATCACAGTAATCAAACC+AGG | 0.647796 | 8.3:+7861952 | None:intergenic |
| GGTTACTGGTTCAAGCAATG+TGG | 0.661828 | 8.3:-7862004 | MS.gene58943:CDS |
| GGGGTAGAGAGTTGGGACAG+TGG | 0.661869 | 8.3:+7862335 | None:intergenic |
| TTGGGATTCCTATTACGACA+CGG | 0.678110 | 8.3:+7862039 | None:intergenic |
| CACTACTACTAAAACCCAAG+AGG | 0.682406 | 8.3:-7862418 | MS.gene58943:CDS |
| GAAATGGGTAACAGAAAACG+TGG | 0.703774 | 8.3:-7862441 | MS.gene58943:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGAAAATAAAAGAGAAAA+TGG | + | chr8.3:7861994-7862013 | None:intergenic | 15.0% |
| !!! | TTTATTTTCATTCTTCATTT+GGG | - | chr8.3:7862002-7862021 | MS.gene58943:CDS | 15.0% |
| !!! | TTTTATTTTCATTCTTCATT+TGG | - | chr8.3:7862001-7862020 | MS.gene58943:CDS | 15.0% |
| !! | AAAATAAAAGAGAAAATGGA+AGG | + | chr8.3:7861990-7862009 | None:intergenic | 20.0% |
| !! | ATAACAAATCAAACCATAAT+GGG | + | chr8.3:7862067-7862086 | None:intergenic | 20.0% |
| !!! | TTTGATTTGTTATCTGAAAT+GGG | - | chr8.3:7862074-7862093 | MS.gene58943:CDS | 20.0% |
| ! | GATAACAAATCAAACCATAA+TGG | + | chr8.3:7862068-7862087 | None:intergenic | 25.0% |
| ! | TAATGATGGTAATTTGATCT+TGG | - | chr8.3:7862403-7862422 | MS.gene58943:CDS | 25.0% |
| !! | TAACAAATCAGTTTCTGAAA+TGG | - | chr8.3:7861824-7861843 | MS.gene58943:CDS | 25.0% |
| !! | TTTGATTACTGTGATTTTGA+AGG | - | chr8.3:7862334-7862353 | MS.gene58943:CDS | 25.0% |
| !!! | AACAAATCAGTTTCTGAAAT+GGG | - | chr8.3:7861825-7861844 | MS.gene58943:CDS | 25.0% |
| !!! | GTTTGATTTGTTATCTGAAA+TGG | - | chr8.3:7862073-7862092 | MS.gene58943:CDS | 25.0% |
| !!! | TGAAATGGGTTTTTGTTTTT+AGG | - | chr8.3:7862088-7862107 | MS.gene58943:CDS | 25.0% |
| AAAATCACAGTAATCAAACC+AGG | + | chr8.3:7862332-7862351 | None:intergenic | 30.0% | |
| AAATCAAAGAAACCTGTTGA+AGG | - | chr8.3:7862200-7862219 | MS.gene58943:intron | 30.0% | |
| AATATTTGATCGACCCATTA+TGG | - | chr8.3:7862051-7862070 | MS.gene58943:CDS | 30.0% | |
| AATCAAAGAAACCTGTTGAA+GGG | - | chr8.3:7862201-7862220 | MS.gene58943:intron | 30.0% | |
| AGGAATGATGTTAGTTACAT+TGG | + | chr8.3:7861970-7861989 | None:intergenic | 30.0% | |
| GAATGATGTTAGTTACATTG+GGG | + | chr8.3:7861968-7861987 | None:intergenic | 30.0% | |
| GGAATGATGTTAGTTACATT+GGG | + | chr8.3:7861969-7861988 | None:intergenic | 30.0% | |
| TGAAGAGAAGAAGAAAATGT+TGG | - | chr8.3:7862160-7862179 | MS.gene58943:CDS | 30.0% | |
| ! | GCTAATGTTTCTGTTAATGA+TGG | - | chr8.3:7862389-7862408 | MS.gene58943:CDS | 30.0% |
| !!! | TTTTTGTTTTTAGGCTACAC+AGG | - | chr8.3:7862097-7862116 | MS.gene58943:CDS | 30.0% |
| AAATTAAAACCCCCTTCAAC+AGG | + | chr8.3:7862215-7862234 | None:intergenic | 35.0% | |
| AATTTCTCTGTTACGGTTAC+TGG | - | chr8.3:7862263-7862282 | MS.gene58943:intron | 35.0% | |
| AGAGAAGAAGAAAATGTTGG+AGG | - | chr8.3:7862163-7862182 | MS.gene58943:CDS | 35.0% | |
| AGTAACCGTAACAGAGAAAT+TGG | + | chr8.3:7862264-7862283 | None:intergenic | 35.0% | |
| ATCAAAGAAACCTGTTGAAG+GGG | - | chr8.3:7862202-7862221 | MS.gene58943:intron | 35.0% | |
| GAATCCCAATTTCTCTGTTA+CGG | - | chr8.3:7862256-7862275 | MS.gene58943:intron | 35.0% | |
| GTAACCGTAACAGAGAAATT+GGG | + | chr8.3:7862263-7862282 | None:intergenic | 35.0% | |
| TAAACGTTGAACGTGAAAGA+AGG | - | chr8.3:7861889-7861908 | MS.gene58943:CDS | 35.0% | |
| ! | ATCACTGCTTTGGTTCAAAA+TGG | - | chr8.3:7862428-7862447 | MS.gene58943:CDS | 35.0% |
| ! | TTCAACGTTTAGAACCTCTT+GGG | + | chr8.3:7861880-7861899 | None:intergenic | 35.0% |
| !! | GTTTTTGAAGAATGAAGCTG+AGG | - | chr8.3:7862358-7862377 | MS.gene58943:CDS | 35.0% |
| !! | TTTCTTTGGGATTGAATCTG+TGG | - | chr8.3:7862301-7862320 | MS.gene58943:intron | 35.0% |
| !!! | TGAAGGGGGTTTTAATTTGT+TGG | - | chr8.3:7862217-7862236 | MS.gene58943:intron | 35.0% |
| ATTGAATCTGTGGCTAAACC+TGG | - | chr8.3:7862311-7862330 | MS.gene58943:intron | 40.0% | |
| CAAGCAATGTGGCTTTCTTT+GGG | - | chr8.3:7862288-7862307 | MS.gene58943:intron | 40.0% | |
| CACTACTACTAAAACCCAAG+AGG | - | chr8.3:7861863-7861882 | MS.gene58943:CDS | 40.0% | |
| GAAAATGTTGGAGGAGATGA+AGG | - | chr8.3:7862172-7862191 | MS.gene58943:CDS | 40.0% | |
| GAAATGGGTAACAGAAAACG+TGG | - | chr8.3:7861840-7861859 | MS.gene58943:CDS | 40.0% | |
| TCAAAGAAACCTGTTGAAGG+GGG | - | chr8.3:7862203-7862222 | MS.gene58943:intron | 40.0% | |
| TCAAGCAATGTGGCTTTCTT+TGG | - | chr8.3:7862287-7862306 | MS.gene58943:intron | 40.0% | |
| TTACATTGGGGTAGAGAGTT+GGG | + | chr8.3:7861956-7861975 | None:intergenic | 40.0% | |
| TTGGGATTCCTATTACGACA+CGG | + | chr8.3:7862245-7862264 | None:intergenic | 40.0% | |
| ! | GTTCAACGTTTAGAACCTCT+TGG | + | chr8.3:7861881-7861900 | None:intergenic | 40.0% |
| GTTACATTGGGGTAGAGAGT+TGG | + | chr8.3:7861957-7861976 | None:intergenic | 45.0% | |
| !! | GGTTACTGGTTCAAGCAATG+TGG | - | chr8.3:7862277-7862296 | MS.gene58943:intron | 45.0% |
| !! | TGTTGGTTCCGTGTCGTAAT+AGG | - | chr8.3:7862234-7862253 | MS.gene58943:intron | 45.0% |
| GATCTTGGCGATCACTGCTT+TGG | - | chr8.3:7862418-7862437 | MS.gene58943:CDS | 50.0% | |
| ! | GAGTTGGGACAGTGGTAGTG+AGG | + | chr8.3:7861941-7861960 | None:intergenic | 55.0% |
| GGGGTAGAGAGTTGGGACAG+TGG | + | chr8.3:7861949-7861968 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 7861810 | 7862493 | 7861810 | ID=MS.gene58943 |
| chr8.3 | mRNA | 7861810 | 7862493 | 7861810 | ID=MS.gene58943.t1;Parent=MS.gene58943 |
| chr8.3 | exon | 7862332 | 7862493 | 7862332 | ID=MS.gene58943.t1.exon1;Parent=MS.gene58943.t1 |
| chr8.3 | CDS | 7862332 | 7862493 | 7862332 | ID=cds.MS.gene58943.t1;Parent=MS.gene58943.t1 |
| chr8.3 | exon | 7861810 | 7862193 | 7861810 | ID=MS.gene58943.t1.exon2;Parent=MS.gene58943.t1 |
| chr8.3 | CDS | 7861810 | 7862193 | 7861810 | ID=cds.MS.gene58943.t1;Parent=MS.gene58943.t1 |
| Gene Sequence |
| Protein sequence |
