Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59066.t1 | XP_003609277.1 | 59.6 | 228 | 39 | 2 | 1 | 175 | 43 | 270 | 1.60E-67 | 265.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59066.t1 | Q9LT81 | 31.2 | 282 | 85 | 7 | 4 | 177 | 95 | 375 | 3.3e-26 | 119.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59066.t1 | G7JVB8 | 59.6 | 228 | 39 | 2 | 1 | 175 | 43 | 270 | 1.1e-67 | 265.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene59066.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59066.t1 | MTR_4g113940 | 59.649 | 228 | 39 | 2 | 1 | 175 | 43 | 270 | 5.75e-87 | 257 |
| MS.gene59066.t1 | MTR_4g113890 | 64.211 | 190 | 42 | 3 | 1 | 174 | 51 | 230 | 1.15e-76 | 229 |
| MS.gene59066.t1 | MTR_4g113860 | 43.388 | 242 | 75 | 2 | 1 | 180 | 71 | 312 | 1.66e-60 | 191 |
| MS.gene59066.t1 | MTR_4g113970 | 46.561 | 189 | 50 | 5 | 1 | 139 | 65 | 252 | 7.92e-43 | 144 |
| MS.gene59066.t1 | MTR_3g050390 | 40.566 | 212 | 89 | 4 | 4 | 180 | 24 | 233 | 8.48e-41 | 138 |
| MS.gene59066.t1 | MTR_3g466460 | 55.932 | 59 | 23 | 1 | 127 | 182 | 46 | 104 | 1.13e-14 | 67.8 |
| MS.gene59066.t1 | MTR_8g066730 | 30.556 | 108 | 59 | 1 | 12 | 103 | 91 | 198 | 4.03e-11 | 60.8 |
| MS.gene59066.t1 | MTR_8g066730 | 30.556 | 108 | 59 | 1 | 12 | 103 | 91 | 198 | 7.35e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59066.t1 | AT3G19360 | 31.449 | 283 | 85 | 7 | 4 | 178 | 95 | 376 | 4.72e-31 | 116 |
| MS.gene59066.t1 | AT3G19360 | 31.449 | 283 | 85 | 7 | 4 | 178 | 95 | 376 | 4.72e-31 | 116 |
Find 43 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCTAGCTTCTGCAAATT+TGG | 0.182814 | 8.4:+14001885 | None:intergenic |
| CCAAGTGTACGCTTCTTCTT+AGG | 0.267354 | 8.4:+14001164 | None:intergenic |
| AGCCAGATCAATCGTATTTA+CGG | 0.289560 | 8.4:-14001131 | MS.gene59066:CDS |
| CTCTGGAAACACACCTCATT+TGG | 0.330065 | 8.4:-14001077 | MS.gene59066:CDS |
| AATTTACAGTTATCTCTATA+TGG | 0.333739 | 8.4:+14001759 | None:intergenic |
| TCAATCGTATTTACGGTGAT+TGG | 0.347052 | 8.4:-14001124 | MS.gene59066:CDS |
| CATACCGGATATTGTTCCTT+TGG | 0.350886 | 8.4:-14001589 | MS.gene59066:CDS |
| ATTTGTACCGAGTTCAGATT+TGG | 0.381030 | 8.4:-14001972 | MS.gene59066:CDS |
| AGACTAAGCTGTGTTGCAAT+TGG | 0.425188 | 8.4:-14001615 | MS.gene59066:CDS |
| TCAACTGCAGCTGAGAAATC+AGG | 0.433581 | 8.4:-14001847 | MS.gene59066:CDS |
| AGCAAGCAAGGGGCACTTAT+CGG | 0.435970 | 8.4:-14001639 | MS.gene59066:CDS |
| AGATTTGGTGCTTGTAGAAA+CGG | 0.437088 | 8.4:-14001957 | MS.gene59066:CDS |
| ACGGTGATTGGATTGATGAT+TGG | 0.443878 | 8.4:-14001112 | MS.gene59066:CDS |
| TCCTTGTTTATAGGCAGAGC+AGG | 0.451853 | 8.4:-14001227 | MS.gene59066:intron |
| CCTAAGAAGAAGCGTACACT+TGG | 0.453400 | 8.4:-14001164 | MS.gene59066:CDS |
| CATTCCAAAGGAACAATATC+CGG | 0.460353 | 8.4:+14001585 | None:intergenic |
| CTAATAATTTGGAAGGCAGC+AGG | 0.471854 | 8.4:-14001672 | MS.gene59066:intron |
| AGCTTCTGCAAATTTGGTGG+TGG | 0.473172 | 8.4:+14001891 | None:intergenic |
| CACCGTAAATACGATTGATC+TGG | 0.481235 | 8.4:+14001129 | None:intergenic |
| GGGCTGTGAACAAGCAAGCA+AGG | 0.482646 | 8.4:-14001651 | MS.gene59066:CDS |
| ATGTACCCGACTTGTGGAAA+AGG | 0.484051 | 8.4:-14001198 | MS.gene59066:CDS |
| TGTGCAAGAAGTATTGCAAT+GGG | 0.488339 | 8.4:-14001791 | MS.gene59066:CDS |
| CTCTTTGCTCATGATGAAGA+AGG | 0.508905 | 8.4:-14001556 | MS.gene59066:intron |
| TGCTCTGATTTGTTTGATTG+AGG | 0.510278 | 8.4:+14002014 | None:intergenic |
| CCTAGCTTCTGCAAATTTGG+TGG | 0.512657 | 8.4:+14001888 | None:intergenic |
| GGCTGTGAACAAGCAAGCAA+GGG | 0.513346 | 8.4:-14001650 | MS.gene59066:CDS |
| TTCTGAATGTACCCGACTTG+TGG | 0.526232 | 8.4:-14001204 | MS.gene59066:CDS |
| TGCAGTTGATCTTCGTTGCA+CGG | 0.530704 | 8.4:+14001861 | None:intergenic |
| ATCATGGAGCTGAAGAGATA+AGG | 0.532117 | 8.4:-14001917 | MS.gene59066:CDS |
| TGTTGCAATTGGAAGCATAC+CGG | 0.563682 | 8.4:-14001604 | MS.gene59066:CDS |
| TGATTTGTTTGATTGAGGCA+AGG | 0.569543 | 8.4:+14002019 | None:intergenic |
| CTGTGCAAGAAGTATTGCAA+TGG | 0.572239 | 8.4:-14001792 | MS.gene59066:CDS |
| TGTTTCCAGAGCATAACAAA+AGG | 0.581785 | 8.4:+14001089 | None:intergenic |
| GAAGAATGCTTATCCAAATG+AGG | 0.582982 | 8.4:+14001064 | None:intergenic |
| TAATAATTTGGAAGGCAGCA+GGG | 0.584634 | 8.4:-14001671 | MS.gene59066:intron |
| CCACCAAATTTGCAGAAGCT+AGG | 0.604649 | 8.4:-14001888 | MS.gene59066:CDS |
| AAAGAGACAGTTCATTCCAA+AGG | 0.613697 | 8.4:+14001573 | None:intergenic |
| GCTGTGAACAAGCAAGCAAG+GGG | 0.637136 | 8.4:-14001649 | MS.gene59066:CDS |
| CCAAATTTGCAGAAGCTAGG+TGG | 0.641093 | 8.4:-14001885 | MS.gene59066:CDS |
| CAAGAAGTATTGCAATGGGG+AGG | 0.655957 | 8.4:-14001787 | MS.gene59066:CDS |
| CTAAGAAGAAGCGTACACTT+GGG | 0.659038 | 8.4:-14001163 | MS.gene59066:CDS |
| ACAAGCACCAAATCTGAACT+CGG | 0.659308 | 8.4:+14001965 | None:intergenic |
| GTGCAAGAAGTATTGCAATG+GGG | 0.763140 | 8.4:-14001790 | MS.gene59066:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATGAAAAAATCATACAA+AGG | - | chr8.4:14001275-14001294 | MS.gene59066:intron | 20.0% |
| !! | AATTTACAGTTATCTCTATA+TGG | + | chr8.4:14001340-14001359 | None:intergenic | 20.0% |
| !!! | CAATAACTCATTTTGATTAT+TGG | - | chr8.4:14001566-14001585 | MS.gene59066:CDS | 20.0% |
| ! | AAAAATTGCAACTTTGATCA+TGG | - | chr8.4:14001163-14001182 | MS.gene59066:CDS | 25.0% |
| ! | ACTTATCGGAACTAATAATT+TGG | - | chr8.4:14001413-14001432 | MS.gene59066:intron | 25.0% |
| !!! | CTCATTTTGATTATTGGTTT+TGG | - | chr8.4:14001572-14001591 | MS.gene59066:CDS | 25.0% |
| !!! | TGTTTCATGTTTGATTTGAA+TGG | - | chr8.4:14001764-14001783 | MS.gene59066:intron | 25.0% |
| ATCGGAACTAATAATTTGGA+AGG | - | chr8.4:14001417-14001436 | MS.gene59066:intron | 30.0% | |
| TAAGTACTCTCTTGATTTCA+AGG | + | chr8.4:14001398-14001417 | None:intergenic | 30.0% | |
| ! | ATGATTCCATCCTTGTTTAT+AGG | - | chr8.4:14001860-14001879 | MS.gene59066:CDS | 30.0% |
| !! | GAAATCAAGAGAGTACTTAT+CGG | - | chr8.4:14001399-14001418 | MS.gene59066:intron | 30.0% |
| AAAGAGACAGTTCATTCCAA+AGG | + | chr8.4:14001526-14001545 | None:intergenic | 35.0% | |
| AGCCAGATCAATCGTATTTA+CGG | - | chr8.4:14001965-14001984 | MS.gene59066:CDS | 35.0% | |
| ATTTGTACCGAGTTCAGATT+TGG | - | chr8.4:14001124-14001143 | MS.gene59066:CDS | 35.0% | |
| CATTCCAAAGGAACAATATC+CGG | + | chr8.4:14001514-14001533 | None:intergenic | 35.0% | |
| TAATAATTTGGAAGGCAGCA+GGG | - | chr8.4:14001425-14001444 | MS.gene59066:intron | 35.0% | |
| TCAATCGTATTTACGGTGAT+TGG | - | chr8.4:14001972-14001991 | MS.gene59066:CDS | 35.0% | |
| TGCAAAAATGTTCCTTCCTA+AGG | - | chr8.4:14001825-14001844 | MS.gene59066:CDS | 35.0% | |
| TGTTTCCAGAGCATAACAAA+AGG | + | chr8.4:14002010-14002029 | None:intergenic | 35.0% | |
| ! | AGGAACATTTTTGCAAGACA+AGG | + | chr8.4:14001820-14001839 | None:intergenic | 35.0% |
| ! | ATTTTTGCAAGACAAGGCAA+AGG | + | chr8.4:14001814-14001833 | None:intergenic | 35.0% |
| ! | TCATTTTCCATCAGAACCTT+AGG | + | chr8.4:14001844-14001863 | None:intergenic | 35.0% |
| ! | TTTTTGCAAGACAAGGCAAA+GGG | + | chr8.4:14001813-14001832 | None:intergenic | 35.0% |
| !! | AGATTTGGTGCTTGTAGAAA+CGG | - | chr8.4:14001139-14001158 | MS.gene59066:CDS | 35.0% |
| !! | TGATTTGTTTGATTGAGGCA+AGG | + | chr8.4:14001080-14001099 | None:intergenic | 35.0% |
| !! | TGCTCTGATTTGTTTGATTG+AGG | + | chr8.4:14001085-14001104 | None:intergenic | 35.0% |
| !! | TGTGCAAGAAGTATTGCAAT+GGG | - | chr8.4:14001305-14001324 | MS.gene59066:intron | 35.0% |
| ACAAGCACCAAATCTGAACT+CGG | + | chr8.4:14001134-14001153 | None:intergenic | 40.0% | |
| AGACTAAGCTGTGTTGCAAT+TGG | - | chr8.4:14001481-14001500 | MS.gene59066:intron | 40.0% | |
| ATCATGGAGCTGAAGAGATA+AGG | - | chr8.4:14001179-14001198 | MS.gene59066:CDS | 40.0% | |
| ATGAAGATCCTGCACAGTTT+AGG | - | chr8.4:14001365-14001384 | MS.gene59066:intron | 40.0% | |
| CACCGTAAATACGATTGATC+TGG | + | chr8.4:14001970-14001989 | None:intergenic | 40.0% | |
| CATACCGGATATTGTTCCTT+TGG | - | chr8.4:14001507-14001526 | MS.gene59066:intron | 40.0% | |
| CTAATAATTTGGAAGGCAGC+AGG | - | chr8.4:14001424-14001443 | MS.gene59066:intron | 40.0% | |
| CTCTTTGCTCATGATGAAGA+AGG | - | chr8.4:14001540-14001559 | MS.gene59066:intron | 40.0% | |
| TGAAGATCCTGCACAGTTTA+GGG | - | chr8.4:14001366-14001385 | MS.gene59066:intron | 40.0% | |
| TTTCCATCAGAACCTTAGGA+AGG | + | chr8.4:14001840-14001859 | None:intergenic | 40.0% | |
| ! | ACGGTGATTGGATTGATGAT+TGG | - | chr8.4:14001984-14002003 | MS.gene59066:CDS | 40.0% |
| ! | CTAAGAAGAAGCGTACACTT+GGG | - | chr8.4:14001933-14001952 | MS.gene59066:CDS | 40.0% |
| ! | CTTCTTCCTTTTCCACAAGT+CGG | + | chr8.4:14001907-14001926 | None:intergenic | 40.0% |
| ! | TGTTGCAATTGGAAGCATAC+CGG | - | chr8.4:14001492-14001511 | MS.gene59066:intron | 40.0% |
| ! | TTCTTCCTTTTCCACAAGTC+GGG | + | chr8.4:14001906-14001925 | None:intergenic | 40.0% |
| !! | CTGTGCAAGAAGTATTGCAA+TGG | - | chr8.4:14001304-14001323 | MS.gene59066:intron | 40.0% |
| !! | GTGCAAGAAGTATTGCAATG+GGG | - | chr8.4:14001306-14001325 | MS.gene59066:intron | 40.0% |
| !!! | ATTGGCCTTTTGTTATGCTC+TGG | - | chr8.4:14002002-14002021 | MS.gene59066:CDS | 40.0% |
| AAGGCAAAGGGAAAACCAGT+AGG | + | chr8.4:14001801-14001820 | None:intergenic | 45.0% | |
| ACCTGCTCTGCCTATAAACA+AGG | + | chr8.4:14001873-14001892 | None:intergenic | 45.0% | |
| ACTGTGCAGGATCTTCATGA+AGG | + | chr8.4:14001363-14001382 | None:intergenic | 45.0% | |
| ATGGAAAATGCTCCTCCTAC+TGG | - | chr8.4:14001783-14001802 | MS.gene59066:CDS | 45.0% | |
| CCAAATTTGCAGAAGCTAGG+TGG | - | chr8.4:14001211-14001230 | MS.gene59066:CDS | 45.0% | |
| CCAAGTGTACGCTTCTTCTT+AGG | + | chr8.4:14001935-14001954 | None:intergenic | 45.0% | |
| CCACCAAATTTGCAGAAGCT+AGG | - | chr8.4:14001208-14001227 | MS.gene59066:CDS | 45.0% | |
| CCACCTAGCTTCTGCAAATT+TGG | + | chr8.4:14001214-14001233 | None:intergenic | 45.0% | |
| CCTAGCTTCTGCAAATTTGG+TGG | + | chr8.4:14001211-14001230 | None:intergenic | 45.0% | |
| GAATCATCCCTAAACTGTGC+AGG | + | chr8.4:14001376-14001395 | None:intergenic | 45.0% | |
| GCTCTGCCTATAAACAAGGA+TGG | + | chr8.4:14001869-14001888 | None:intergenic | 45.0% | |
| TCAACTGCAGCTGAGAAATC+AGG | - | chr8.4:14001249-14001268 | MS.gene59066:intron | 45.0% | |
| TGCAGTTGATCTTCGTTGCA+CGG | + | chr8.4:14001238-14001257 | None:intergenic | 45.0% | |
| ! | AGCTTCTGCAAATTTGGTGG+TGG | + | chr8.4:14001208-14001227 | None:intergenic | 45.0% |
| ! | ATGTACCCGACTTGTGGAAA+AGG | - | chr8.4:14001898-14001917 | MS.gene59066:CDS | 45.0% |
| ! | CCTAAGAAGAAGCGTACACT+TGG | - | chr8.4:14001932-14001951 | MS.gene59066:CDS | 45.0% |
| ! | CTCTGGAAACACACCTCATT+TGG | - | chr8.4:14002019-14002038 | MS.gene59066:CDS | 45.0% |
| ! | GTTCCTTCCTAAGGTTCTGA+TGG | - | chr8.4:14001834-14001853 | MS.gene59066:CDS | 45.0% |
| ! | TCCTTGTTTATAGGCAGAGC+AGG | - | chr8.4:14001869-14001888 | MS.gene59066:CDS | 45.0% |
| ! | TTCTGAATGTACCCGACTTG+TGG | - | chr8.4:14001892-14001911 | MS.gene59066:CDS | 45.0% |
| !! | CAAGAAGTATTGCAATGGGG+AGG | - | chr8.4:14001309-14001328 | MS.gene59066:intron | 45.0% |
| GCAAAGGGAAAACCAGTAGG+AGG | + | chr8.4:14001798-14001817 | None:intergenic | 50.0% | |
| GCTGTGAACAAGCAAGCAAG+GGG | - | chr8.4:14001447-14001466 | MS.gene59066:intron | 50.0% | |
| GGCTGTGAACAAGCAAGCAA+GGG | - | chr8.4:14001446-14001465 | MS.gene59066:intron | 50.0% | |
| !! | AGCAAGCAAGGGGCACTTAT+CGG | - | chr8.4:14001457-14001476 | MS.gene59066:intron | 50.0% |
| GGGCTGTGAACAAGCAAGCA+AGG | - | chr8.4:14001445-14001464 | MS.gene59066:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14001073 | 14002045 | 14001073 | ID=MS.gene59066 |
| chr8.4 | mRNA | 14001073 | 14002045 | 14001073 | ID=MS.gene59066.t1;Parent=MS.gene59066 |
| chr8.4 | exon | 14001766 | 14002045 | 14001766 | ID=MS.gene59066.t1.exon1;Parent=MS.gene59066.t1 |
| chr8.4 | CDS | 14001766 | 14002045 | 14001766 | ID=cds.MS.gene59066.t1;Parent=MS.gene59066.t1 |
| chr8.4 | exon | 14001557 | 14001679 | 14001557 | ID=MS.gene59066.t1.exon2;Parent=MS.gene59066.t1 |
| chr8.4 | CDS | 14001557 | 14001679 | 14001557 | ID=cds.MS.gene59066.t1;Parent=MS.gene59066.t1 |
| chr8.4 | exon | 14001073 | 14001236 | 14001073 | ID=MS.gene59066.t1.exon3;Parent=MS.gene59066.t1 |
| chr8.4 | CDS | 14001073 | 14001236 | 14001073 | ID=cds.MS.gene59066.t1;Parent=MS.gene59066.t1 |
| Gene Sequence |
| Protein sequence |
