Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59068.t1 | XP_003609277.1 | 57 | 270 | 43 | 4 | 1 | 197 | 1 | 270 | 1.80E-72 | 282.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59068.t1 | Q9LT81 | 29.9 | 261 | 74 | 7 | 47 | 199 | 116 | 375 | 3.4e-19 | 96.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59068.t1 | G7JVB8 | 57.0 | 270 | 43 | 4 | 1 | 197 | 1 | 270 | 1.3e-72 | 282.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene59068.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59068.t1 | MTR_4g113940 | 57.037 | 270 | 43 | 4 | 1 | 197 | 1 | 270 | 5.31e-94 | 275 |
| MS.gene59068.t1 | MTR_4g113890 | 60.000 | 240 | 42 | 4 | 1 | 196 | 1 | 230 | 1.03e-87 | 258 |
| MS.gene59068.t1 | MTR_4g113860 | 36.218 | 312 | 89 | 5 | 1 | 202 | 1 | 312 | 2.56e-54 | 176 |
| MS.gene59068.t1 | MTR_4g113970 | 39.130 | 253 | 61 | 6 | 1 | 161 | 1 | 252 | 1.55e-41 | 141 |
| MS.gene59068.t1 | MTR_3g050390 | 36.652 | 221 | 89 | 4 | 31 | 202 | 15 | 233 | 6.06e-34 | 121 |
| MS.gene59068.t1 | MTR_3g466460 | 55.932 | 59 | 23 | 1 | 149 | 204 | 46 | 104 | 1.93e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59068.t1 | AT3G19360 | 30.153 | 262 | 74 | 7 | 47 | 200 | 116 | 376 | 4.86e-23 | 95.5 |
| MS.gene59068.t1 | AT3G19360 | 30.153 | 262 | 74 | 7 | 47 | 200 | 116 | 376 | 4.86e-23 | 95.5 |
Find 52 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCTAGCTTCTGCAAATT+TGG | 0.182814 | 8.4:+14012007 | None:intergenic |
| CCAAGTGTACGCTTCTTCTT+AGG | 0.267354 | 8.4:+14011286 | None:intergenic |
| AGCCAGATCAATCGTATTTA+CGG | 0.289560 | 8.4:-14011253 | MS.gene59068:CDS |
| CTCTGGAAACACACCTCATT+TGG | 0.330065 | 8.4:-14011199 | MS.gene59068:CDS |
| AATTTACAGTTATCTCTATA+TGG | 0.333739 | 8.4:+14011881 | None:intergenic |
| TCAATCGTATTTACGGTGAT+TGG | 0.347052 | 8.4:-14011246 | MS.gene59068:CDS |
| CATACCGGATATTGTTCCTT+TGG | 0.350886 | 8.4:-14011711 | MS.gene59068:CDS |
| TGAACAATAATGACTTCAAA+AGG | 0.385432 | 8.4:-14012237 | MS.gene59068:CDS |
| AGACTAAGCTGTGTTGCAAT+TGG | 0.425188 | 8.4:-14011737 | MS.gene59068:CDS |
| TCAACTGCAGCTGAGAAATC+AGG | 0.433581 | 8.4:-14011969 | MS.gene59068:CDS |
| AGCAAGCAAGGGGCACTTAT+CGG | 0.435970 | 8.4:-14011761 | MS.gene59068:CDS |
| AGATTTGGTGCTTGTAGAAA+CGG | 0.437088 | 8.4:-14012079 | MS.gene59068:CDS |
| TTAGAGGTTGGAATATCATA+TGG | 0.441570 | 8.4:+14012286 | None:intergenic |
| ACGGTGATTGGATTGATGAT+TGG | 0.443878 | 8.4:-14011234 | MS.gene59068:CDS |
| TTGCGGCGGTGACATCTTAG+AGG | 0.444480 | 8.4:+14012270 | None:intergenic |
| TCCTTGTTTATAGGCAGAGC+AGG | 0.451853 | 8.4:-14011349 | MS.gene59068:intron |
| CCTAAGAAGAAGCGTACACT+TGG | 0.453400 | 8.4:-14011286 | MS.gene59068:CDS |
| CATTCCAAAGGAACAATATC+CGG | 0.460353 | 8.4:+14011707 | None:intergenic |
| CTAATAATTTGGAAGGCAGC+AGG | 0.471854 | 8.4:-14011794 | MS.gene59068:intron |
| AGCTTCTGCAAATTTGGTGG+TGG | 0.473172 | 8.4:+14012013 | None:intergenic |
| CACCGTAAATACGATTGATC+TGG | 0.481235 | 8.4:+14011251 | None:intergenic |
| GGGCTGTGAACAAGCAAGCA+AGG | 0.482646 | 8.4:-14011773 | MS.gene59068:CDS |
| ATGTACCCGACTTGTGGAAA+AGG | 0.484051 | 8.4:-14011320 | MS.gene59068:CDS |
| TGTGCAAGAAGTATTGCAAT+GGG | 0.488339 | 8.4:-14011913 | MS.gene59068:CDS |
| CTCTTTGCTCATGATGAAGA+AGG | 0.508905 | 8.4:-14011678 | MS.gene59068:intron |
| CCTAGCTTCTGCAAATTTGG+TGG | 0.512657 | 8.4:+14012010 | None:intergenic |
| GGCTGTGAACAAGCAAGCAA+GGG | 0.513346 | 8.4:-14011772 | MS.gene59068:CDS |
| TTCTGAATGTACCCGACTTG+TGG | 0.526232 | 8.4:-14011326 | MS.gene59068:CDS |
| TGCAGTTGATCTTCGTTGCA+CGG | 0.530704 | 8.4:+14011983 | None:intergenic |
| ATCATGGAGCTGAAGAGATA+AGG | 0.532117 | 8.4:-14012039 | MS.gene59068:CDS |
| AGGAGGGCATATCAATGCAT+TGG | 0.539478 | 8.4:+14012198 | None:intergenic |
| GAACAATAATGACTTCAAAA+GGG | 0.552156 | 8.4:-14012236 | MS.gene59068:CDS |
| GGCGGTGACATCTTAGAGGT+TGG | 0.560713 | 8.4:+14012274 | None:intergenic |
| TGTTGCAATTGGAAGCATAC+CGG | 0.563682 | 8.4:-14011726 | MS.gene59068:CDS |
| CTGTGCAAGAAGTATTGCAA+TGG | 0.572239 | 8.4:-14011914 | MS.gene59068:CDS |
| TGTTTCCAGAGCATAACAAA+AGG | 0.581785 | 8.4:+14011211 | None:intergenic |
| AATAATGACTTCAAAAGGGA+AGG | 0.582018 | 8.4:-14012232 | MS.gene59068:CDS |
| GAAGAATGCTTATCCAAATG+AGG | 0.582982 | 8.4:+14011186 | None:intergenic |
| TAATAATTTGGAAGGCAGCA+GGG | 0.584634 | 8.4:-14011793 | MS.gene59068:intron |
| CACATCAATGCATCAGAAGG+AGG | 0.586467 | 8.4:+14012181 | None:intergenic |
| TTGTTCAATGAAGTGAATTG+CGG | 0.591801 | 8.4:+14012253 | None:intergenic |
| CCACCAAATTTGCAGAAGCT+AGG | 0.604649 | 8.4:-14012010 | MS.gene59068:CDS |
| AAAGAGACAGTTCATTCCAA+AGG | 0.613697 | 8.4:+14011695 | None:intergenic |
| GGTCACATCAATGCATCAGA+AGG | 0.614755 | 8.4:+14012178 | None:intergenic |
| ACATCAATGCATCAGAAGGA+GGG | 0.627126 | 8.4:+14012182 | None:intergenic |
| GCTGTGAACAAGCAAGCAAG+GGG | 0.637136 | 8.4:-14011771 | MS.gene59068:CDS |
| AATATCATATGGAAAAGCCA+TGG | 0.637338 | 8.4:+14012297 | None:intergenic |
| CCAAATTTGCAGAAGCTAGG+TGG | 0.641093 | 8.4:-14012007 | MS.gene59068:CDS |
| CAAGAAGTATTGCAATGGGG+AGG | 0.655957 | 8.4:-14011909 | MS.gene59068:CDS |
| CTAAGAAGAAGCGTACACTT+GGG | 0.659038 | 8.4:-14011285 | MS.gene59068:CDS |
| TTCAATGAAGTGAATTGCGG+CGG | 0.670436 | 8.4:+14012256 | None:intergenic |
| GTGCAAGAAGTATTGCAATG+GGG | 0.763140 | 8.4:-14011912 | MS.gene59068:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATGAAAAAATCATACAA+AGG | - | chr8.4:14011547-14011566 | MS.gene59068:intron | 20.0% |
| !! | AATTTACAGTTATCTCTATA+TGG | + | chr8.4:14011612-14011631 | None:intergenic | 20.0% |
| !!! | CAATAACTCATTTTGATTAT+TGG | - | chr8.4:14011838-14011857 | MS.gene59068:intron | 20.0% |
| ! | AAAAATTGCAACTTTGATCA+TGG | - | chr8.4:14011435-14011454 | MS.gene59068:intron | 25.0% |
| ! | ACTTATCGGAACTAATAATT+TGG | - | chr8.4:14011685-14011704 | MS.gene59068:CDS | 25.0% |
| ! | GAACAATAATGACTTCAAAA+GGG | - | chr8.4:14011254-14011273 | MS.gene59068:CDS | 25.0% |
| ! | TGAACAATAATGACTTCAAA+AGG | - | chr8.4:14011253-14011272 | MS.gene59068:CDS | 25.0% |
| !!! | CTCATTTTGATTATTGGTTT+TGG | - | chr8.4:14011844-14011863 | MS.gene59068:intron | 25.0% |
| !!! | TGTTTCATGTTTGATTTGAA+TGG | - | chr8.4:14012036-14012055 | MS.gene59068:CDS | 25.0% |
| AATAATGACTTCAAAAGGGA+AGG | - | chr8.4:14011258-14011277 | MS.gene59068:CDS | 30.0% | |
| ATCGGAACTAATAATTTGGA+AGG | - | chr8.4:14011689-14011708 | MS.gene59068:CDS | 30.0% | |
| TAAGTACTCTCTTGATTTCA+AGG | + | chr8.4:14011670-14011689 | None:intergenic | 30.0% | |
| TTAGAGGTTGGAATATCATA+TGG | + | chr8.4:14011207-14011226 | None:intergenic | 30.0% | |
| TTGTTCAATGAAGTGAATTG+CGG | + | chr8.4:14011240-14011259 | None:intergenic | 30.0% | |
| ! | ATGATTCCATCCTTGTTTAT+AGG | - | chr8.4:14012132-14012151 | MS.gene59068:intron | 30.0% |
| !! | GAAATCAAGAGAGTACTTAT+CGG | - | chr8.4:14011671-14011690 | MS.gene59068:intron | 30.0% |
| AAAGAGACAGTTCATTCCAA+AGG | + | chr8.4:14011798-14011817 | None:intergenic | 35.0% | |
| AGCCAGATCAATCGTATTTA+CGG | - | chr8.4:14012237-14012256 | MS.gene59068:CDS | 35.0% | |
| ATTTGTACCGAGTTCAGATT+TGG | - | chr8.4:14011396-14011415 | MS.gene59068:intron | 35.0% | |
| CATTCCAAAGGAACAATATC+CGG | + | chr8.4:14011786-14011805 | None:intergenic | 35.0% | |
| TAATAATTTGGAAGGCAGCA+GGG | - | chr8.4:14011697-14011716 | MS.gene59068:CDS | 35.0% | |
| TCAATCGTATTTACGGTGAT+TGG | - | chr8.4:14012244-14012263 | MS.gene59068:CDS | 35.0% | |
| TGCAAAAATGTTCCTTCCTA+AGG | - | chr8.4:14012097-14012116 | MS.gene59068:intron | 35.0% | |
| TGTTTCCAGAGCATAACAAA+AGG | + | chr8.4:14012282-14012301 | None:intergenic | 35.0% | |
| ! | AGGAACATTTTTGCAAGACA+AGG | + | chr8.4:14012092-14012111 | None:intergenic | 35.0% |
| ! | ATTTTTGCAAGACAAGGCAA+AGG | + | chr8.4:14012086-14012105 | None:intergenic | 35.0% |
| ! | TCATTTTCCATCAGAACCTT+AGG | + | chr8.4:14012116-14012135 | None:intergenic | 35.0% |
| ! | TTTTTGCAAGACAAGGCAAA+GGG | + | chr8.4:14012085-14012104 | None:intergenic | 35.0% |
| !! | AGATTTGGTGCTTGTAGAAA+CGG | - | chr8.4:14011411-14011430 | MS.gene59068:intron | 35.0% |
| !! | TGATTTGTTTGATTGAGGCA+AGG | + | chr8.4:14011352-14011371 | None:intergenic | 35.0% |
| !! | TGCTCTGATTTGTTTGATTG+AGG | + | chr8.4:14011357-14011376 | None:intergenic | 35.0% |
| !! | TGTGCAAGAAGTATTGCAAT+GGG | - | chr8.4:14011577-14011596 | MS.gene59068:intron | 35.0% |
| ACAAGCACCAAATCTGAACT+CGG | + | chr8.4:14011406-14011425 | None:intergenic | 40.0% | |
| ACATCAATGCATCAGAAGGA+GGG | + | chr8.4:14011311-14011330 | None:intergenic | 40.0% | |
| AGACTAAGCTGTGTTGCAAT+TGG | - | chr8.4:14011753-14011772 | MS.gene59068:CDS | 40.0% | |
| ATCATGGAGCTGAAGAGATA+AGG | - | chr8.4:14011451-14011470 | MS.gene59068:intron | 40.0% | |
| ATGAAGATCCTGCACAGTTT+AGG | - | chr8.4:14011637-14011656 | MS.gene59068:intron | 40.0% | |
| CACCGTAAATACGATTGATC+TGG | + | chr8.4:14012242-14012261 | None:intergenic | 40.0% | |
| CATACCGGATATTGTTCCTT+TGG | - | chr8.4:14011779-14011798 | MS.gene59068:CDS | 40.0% | |
| CTAATAATTTGGAAGGCAGC+AGG | - | chr8.4:14011696-14011715 | MS.gene59068:CDS | 40.0% | |
| CTCTTTGCTCATGATGAAGA+AGG | - | chr8.4:14011812-14011831 | MS.gene59068:intron | 40.0% | |
| TGAAGATCCTGCACAGTTTA+GGG | - | chr8.4:14011638-14011657 | MS.gene59068:intron | 40.0% | |
| TTCAATGAAGTGAATTGCGG+CGG | + | chr8.4:14011237-14011256 | None:intergenic | 40.0% | |
| TTTCCATCAGAACCTTAGGA+AGG | + | chr8.4:14012112-14012131 | None:intergenic | 40.0% | |
| ! | ACGGTGATTGGATTGATGAT+TGG | - | chr8.4:14012256-14012275 | MS.gene59068:CDS | 40.0% |
| ! | CTAAGAAGAAGCGTACACTT+GGG | - | chr8.4:14012205-14012224 | MS.gene59068:CDS | 40.0% |
| ! | CTTCTTCCTTTTCCACAAGT+CGG | + | chr8.4:14012179-14012198 | None:intergenic | 40.0% |
| ! | TGTTGCAATTGGAAGCATAC+CGG | - | chr8.4:14011764-14011783 | MS.gene59068:CDS | 40.0% |
| ! | TTCTTCCTTTTCCACAAGTC+GGG | + | chr8.4:14012178-14012197 | None:intergenic | 40.0% |
| !! | CTGTGCAAGAAGTATTGCAA+TGG | - | chr8.4:14011576-14011595 | MS.gene59068:intron | 40.0% |
| !! | GTGCAAGAAGTATTGCAATG+GGG | - | chr8.4:14011578-14011597 | MS.gene59068:intron | 40.0% |
| !!! | ATTGGCCTTTTGTTATGCTC+TGG | - | chr8.4:14012274-14012293 | MS.gene59068:CDS | 40.0% |
| AAGGCAAAGGGAAAACCAGT+AGG | + | chr8.4:14012073-14012092 | None:intergenic | 45.0% | |
| ACCTGCTCTGCCTATAAACA+AGG | + | chr8.4:14012145-14012164 | None:intergenic | 45.0% | |
| ACTGTGCAGGATCTTCATGA+AGG | + | chr8.4:14011635-14011654 | None:intergenic | 45.0% | |
| ATGGAAAATGCTCCTCCTAC+TGG | - | chr8.4:14012055-14012074 | MS.gene59068:CDS | 45.0% | |
| CACATCAATGCATCAGAAGG+AGG | + | chr8.4:14011312-14011331 | None:intergenic | 45.0% | |
| CCAAATTTGCAGAAGCTAGG+TGG | - | chr8.4:14011483-14011502 | MS.gene59068:intron | 45.0% | |
| CCAAGTGTACGCTTCTTCTT+AGG | + | chr8.4:14012207-14012226 | None:intergenic | 45.0% | |
| CCACCAAATTTGCAGAAGCT+AGG | - | chr8.4:14011480-14011499 | MS.gene59068:intron | 45.0% | |
| CCACCTAGCTTCTGCAAATT+TGG | + | chr8.4:14011486-14011505 | None:intergenic | 45.0% | |
| CCTAGCTTCTGCAAATTTGG+TGG | + | chr8.4:14011483-14011502 | None:intergenic | 45.0% | |
| GAATCATCCCTAAACTGTGC+AGG | + | chr8.4:14011648-14011667 | None:intergenic | 45.0% | |
| GCTCTGCCTATAAACAAGGA+TGG | + | chr8.4:14012141-14012160 | None:intergenic | 45.0% | |
| GGTCACATCAATGCATCAGA+AGG | + | chr8.4:14011315-14011334 | None:intergenic | 45.0% | |
| TCAACTGCAGCTGAGAAATC+AGG | - | chr8.4:14011521-14011540 | MS.gene59068:intron | 45.0% | |
| TGCAGTTGATCTTCGTTGCA+CGG | + | chr8.4:14011510-14011529 | None:intergenic | 45.0% | |
| ! | AGCTTCTGCAAATTTGGTGG+TGG | + | chr8.4:14011480-14011499 | None:intergenic | 45.0% |
| ! | AGGAGGGCATATCAATGCAT+TGG | + | chr8.4:14011295-14011314 | None:intergenic | 45.0% |
| ! | ATGTACCCGACTTGTGGAAA+AGG | - | chr8.4:14012170-14012189 | MS.gene59068:intron | 45.0% |
| ! | CCTAAGAAGAAGCGTACACT+TGG | - | chr8.4:14012204-14012223 | MS.gene59068:CDS | 45.0% |
| ! | CTCTGGAAACACACCTCATT+TGG | - | chr8.4:14012291-14012310 | MS.gene59068:CDS | 45.0% |
| ! | GTTCCTTCCTAAGGTTCTGA+TGG | - | chr8.4:14012106-14012125 | MS.gene59068:intron | 45.0% |
| ! | TCCTTGTTTATAGGCAGAGC+AGG | - | chr8.4:14012141-14012160 | MS.gene59068:intron | 45.0% |
| ! | TTCTGAATGTACCCGACTTG+TGG | - | chr8.4:14012164-14012183 | MS.gene59068:intron | 45.0% |
| !! | CAAGAAGTATTGCAATGGGG+AGG | - | chr8.4:14011581-14011600 | MS.gene59068:intron | 45.0% |
| CATTGATGTGACCTCCTCCA+AGG | - | chr8.4:14011322-14011341 | MS.gene59068:CDS | 50.0% | |
| GCAAAGGGAAAACCAGTAGG+AGG | + | chr8.4:14012070-14012089 | None:intergenic | 50.0% | |
| GCTGTGAACAAGCAAGCAAG+GGG | - | chr8.4:14011719-14011738 | MS.gene59068:CDS | 50.0% | |
| GGCTGTGAACAAGCAAGCAA+GGG | - | chr8.4:14011718-14011737 | MS.gene59068:CDS | 50.0% | |
| !! | AGCAAGCAAGGGGCACTTAT+CGG | - | chr8.4:14011729-14011748 | MS.gene59068:CDS | 50.0% |
| GATGTGACCTCCTCCAAGGA+TGG | - | chr8.4:14011326-14011345 | MS.gene59068:CDS | 55.0% | |
| GATTGAGGCAAGGCCATCCT+TGG | + | chr8.4:14011342-14011361 | None:intergenic | 55.0% | |
| GGCGGTGACATCTTAGAGGT+TGG | + | chr8.4:14011219-14011238 | None:intergenic | 55.0% | |
| GGGCTGTGAACAAGCAAGCA+AGG | - | chr8.4:14011717-14011736 | MS.gene59068:CDS | 55.0% | |
| TTGCGGCGGTGACATCTTAG+AGG | + | chr8.4:14011223-14011242 | None:intergenic | 55.0% | |
| TGAGGCAAGGCCATCCTTGG+AGG | + | chr8.4:14011339-14011358 | None:intergenic | 60.0% | |
| GGCAAGGCCATCCTTGGAGG+AGG | + | chr8.4:14011336-14011355 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14011195 | 14012317 | 14011195 | ID=MS.gene59068 |
| chr8.4 | mRNA | 14011195 | 14012317 | 14011195 | ID=MS.gene59068.t1;Parent=MS.gene59068 |
| chr8.4 | exon | 14012184 | 14012317 | 14012184 | ID=MS.gene59068.t1.exon1;Parent=MS.gene59068.t1 |
| chr8.4 | CDS | 14012184 | 14012317 | 14012184 | ID=cds.MS.gene59068.t1;Parent=MS.gene59068.t1 |
| chr8.4 | exon | 14011888 | 14012099 | 14011888 | ID=MS.gene59068.t1.exon2;Parent=MS.gene59068.t1 |
| chr8.4 | CDS | 14011888 | 14012099 | 14011888 | ID=cds.MS.gene59068.t1;Parent=MS.gene59068.t1 |
| chr8.4 | exon | 14011679 | 14011801 | 14011679 | ID=MS.gene59068.t1.exon3;Parent=MS.gene59068.t1 |
| chr8.4 | CDS | 14011679 | 14011801 | 14011679 | ID=cds.MS.gene59068.t1;Parent=MS.gene59068.t1 |
| chr8.4 | exon | 14011195 | 14011358 | 14011195 | ID=MS.gene59068.t1.exon4;Parent=MS.gene59068.t1 |
| chr8.4 | CDS | 14011195 | 14011358 | 14011195 | ID=cds.MS.gene59068.t1;Parent=MS.gene59068.t1 |
| Gene Sequence |
| Protein sequence |
