Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59071.t1 | XP_003609277.1 | 46.7 | 259 | 65 | 4 | 1 | 186 | 12 | 270 | 1.70E-48 | 202.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59071.t1 | Q9LT81 | 43.3 | 90 | 32 | 1 | 36 | 106 | 116 | 205 | 4.0e-14 | 79.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59071.t1 | G7JVB8 | 46.7 | 259 | 65 | 4 | 1 | 186 | 12 | 270 | 1.2e-48 | 202.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene59071.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59071.t1 | MTR_4g113940 | 56.371 | 259 | 40 | 4 | 1 | 186 | 12 | 270 | 6.00e-89 | 262 |
| MS.gene59071.t1 | MTR_4g113890 | 58.515 | 229 | 41 | 4 | 1 | 185 | 12 | 230 | 2.49e-80 | 239 |
| MS.gene59071.t1 | MTR_4g113860 | 34.551 | 301 | 87 | 5 | 1 | 191 | 12 | 312 | 6.53e-47 | 157 |
| MS.gene59071.t1 | MTR_4g113970 | 38.017 | 242 | 57 | 6 | 1 | 150 | 12 | 252 | 2.42e-36 | 127 |
| MS.gene59071.t1 | MTR_3g050390 | 36.652 | 221 | 89 | 4 | 20 | 191 | 15 | 233 | 3.73e-34 | 121 |
| MS.gene59071.t1 | MTR_3g466460 | 55.932 | 59 | 23 | 1 | 138 | 193 | 46 | 104 | 1.62e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59071.t1 | AT3G19360 | 30.153 | 262 | 74 | 7 | 36 | 189 | 116 | 376 | 4.20e-23 | 95.5 |
| MS.gene59071.t1 | AT3G19360 | 30.153 | 262 | 74 | 7 | 36 | 189 | 116 | 376 | 4.20e-23 | 95.5 |
Find 49 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCTAGCTTCTGCAAATT+TGG | 0.182814 | 8.4:+14026175 | None:intergenic |
| CCAAGTGTACGCTTCTTCTT+AGG | 0.267354 | 8.4:+14025454 | None:intergenic |
| AGCCAGATCAATCGTATTTA+CGG | 0.289560 | 8.4:-14025421 | MS.gene59071:CDS |
| CTCTGGAAACACACCTCATT+TGG | 0.330065 | 8.4:-14025367 | MS.gene59071:CDS |
| AATTTACAGTTATCTCTATA+TGG | 0.333739 | 8.4:+14026049 | None:intergenic |
| TCAATCGTATTTACGGTGAT+TGG | 0.347052 | 8.4:-14025414 | MS.gene59071:CDS |
| CATACCGGATATTGTTCCTT+TGG | 0.350886 | 8.4:-14025879 | MS.gene59071:CDS |
| TGAACAATAATGACTTCAAA+AGG | 0.385432 | 8.4:-14026405 | MS.gene59071:CDS |
| AGACTAAGCTGTGTCGCAAT+TGG | 0.427484 | 8.4:-14025905 | MS.gene59071:CDS |
| AGCAAGCAAGGGGCACTTAT+CGG | 0.435970 | 8.4:-14025929 | MS.gene59071:CDS |
| AGATTTGGTGCTTGTAGAAA+CGG | 0.437088 | 8.4:-14026247 | MS.gene59071:CDS |
| ACGGTGATTGGATTGATGAT+TGG | 0.443878 | 8.4:-14025402 | MS.gene59071:CDS |
| CAACGAAGATCAACTGCAGC+TGG | 0.445811 | 8.4:-14026146 | MS.gene59071:CDS |
| TCCTTGTTTATAGGCAGAGC+AGG | 0.451853 | 8.4:-14025517 | MS.gene59071:intron |
| CCTAAGAAGAAGCGTACACT+TGG | 0.453400 | 8.4:-14025454 | MS.gene59071:CDS |
| CATTCCAAAGGAACAATATC+CGG | 0.460353 | 8.4:+14025875 | None:intergenic |
| CTAATAATTTGGAAGGCAGC+AGG | 0.471854 | 8.4:-14025962 | MS.gene59071:intron |
| AGCTTCTGCAAATTTGGTGG+TGG | 0.473172 | 8.4:+14026181 | None:intergenic |
| CACCGTAAATACGATTGATC+TGG | 0.481235 | 8.4:+14025419 | None:intergenic |
| GGGCTGTGAACAAGCAAGCA+AGG | 0.482646 | 8.4:-14025941 | MS.gene59071:CDS |
| ATGTACCCGACTTGTGGAAA+AGG | 0.484051 | 8.4:-14025488 | MS.gene59071:CDS |
| TGTGCAAGAAGTATTGCAAT+GGG | 0.488339 | 8.4:-14026081 | MS.gene59071:CDS |
| CTCTTTGCTCATGATGAAGA+AGG | 0.508905 | 8.4:-14025846 | MS.gene59071:intron |
| CCTAGCTTCTGCAAATTTGG+TGG | 0.512657 | 8.4:+14026178 | None:intergenic |
| GGCTGTGAACAAGCAAGCAA+GGG | 0.513346 | 8.4:-14025940 | MS.gene59071:CDS |
| TTCTGAATGTACCCGACTTG+TGG | 0.526232 | 8.4:-14025494 | MS.gene59071:CDS |
| TGCAGTTGATCTTCGTTGCA+CGG | 0.530704 | 8.4:+14026151 | None:intergenic |
| ATCATGGAGCTGAAGAGATA+AGG | 0.532117 | 8.4:-14026207 | MS.gene59071:CDS |
| AGGAGGGCATATCAATGCAT+TGG | 0.539478 | 8.4:+14026366 | None:intergenic |
| GAACAATAATGACTTCAAAA+GGG | 0.552156 | 8.4:-14026404 | MS.gene59071:CDS |
| TGTCGCAATTGGAAGCATAC+CGG | 0.563682 | 8.4:-14025894 | MS.gene59071:CDS |
| CTGTGCAAGAAGTATTGCAA+TGG | 0.572239 | 8.4:-14026082 | MS.gene59071:CDS |
| TGTTTCCAGAGCATAACAAA+AGG | 0.581785 | 8.4:+14025379 | None:intergenic |
| AATAATGACTTCAAAAGGGA+AGG | 0.582018 | 8.4:-14026400 | MS.gene59071:CDS |
| GAAGAATGCTTATCCAAATG+AGG | 0.582982 | 8.4:+14025354 | None:intergenic |
| TAATAATTTGGAAGGCAGCA+GGG | 0.584634 | 8.4:-14025961 | MS.gene59071:intron |
| CACATCAATGCATCAGAAGG+AGG | 0.586467 | 8.4:+14026349 | None:intergenic |
| TTGTTCAATGAAGTGAATTG+CGG | 0.591801 | 8.4:+14026421 | None:intergenic |
| AACGAAGATCAACTGCAGCT+GGG | 0.593604 | 8.4:-14026145 | MS.gene59071:CDS |
| CCACCAAATTTGCAGAAGCT+AGG | 0.604649 | 8.4:-14026178 | MS.gene59071:CDS |
| AAAGAGACAGTTCATTCCAA+AGG | 0.613697 | 8.4:+14025863 | None:intergenic |
| GGTCACATCAATGCATCAGA+AGG | 0.614755 | 8.4:+14026346 | None:intergenic |
| ACATCAATGCATCAGAAGGA+GGG | 0.627126 | 8.4:+14026350 | None:intergenic |
| GCTGTGAACAAGCAAGCAAG+GGG | 0.637136 | 8.4:-14025939 | MS.gene59071:CDS |
| CCAAATTTGCAGAAGCTAGG+TGG | 0.641093 | 8.4:-14026175 | MS.gene59071:CDS |
| CAAGAAGTATTGCAATGGGG+AGG | 0.655957 | 8.4:-14026077 | MS.gene59071:CDS |
| CTAAGAAGAAGCGTACACTT+GGG | 0.659038 | 8.4:-14025453 | MS.gene59071:CDS |
| TTCAATGAAGTGAATTGCGG+CGG | 0.670436 | 8.4:+14026424 | None:intergenic |
| GTGCAAGAAGTATTGCAATG+GGG | 0.763140 | 8.4:-14026080 | MS.gene59071:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATGAAAAAATCATACAA+AGG | - | chr8.4:14025682-14025701 | MS.gene59071:intron | 20.0% |
| !! | AATTTACAGTTATCTCTATA+TGG | + | chr8.4:14025747-14025766 | None:intergenic | 20.0% |
| !!! | CAATAACTCATTTTGATTAT+TGG | - | chr8.4:14025973-14025992 | MS.gene59071:intron | 20.0% |
| ! | AAAAATTGCAACTTTGATCA+TGG | - | chr8.4:14025570-14025589 | MS.gene59071:intron | 25.0% |
| ! | ACTTATCGGAACTAATAATT+TGG | - | chr8.4:14025820-14025839 | MS.gene59071:intron | 25.0% |
| ! | GAACAATAATGACTTCAAAA+GGG | - | chr8.4:14025389-14025408 | MS.gene59071:CDS | 25.0% |
| ! | TGAACAATAATGACTTCAAA+AGG | - | chr8.4:14025388-14025407 | MS.gene59071:CDS | 25.0% |
| !!! | CTCATTTTGATTATTGGTTT+TGG | - | chr8.4:14025979-14025998 | MS.gene59071:intron | 25.0% |
| !!! | TGTTTCATGTTTGATTTGAA+TGG | - | chr8.4:14026171-14026190 | MS.gene59071:CDS | 25.0% |
| AATAATGACTTCAAAAGGGA+AGG | - | chr8.4:14025393-14025412 | MS.gene59071:CDS | 30.0% | |
| ATCGGAACTAATAATTTGGA+AGG | - | chr8.4:14025824-14025843 | MS.gene59071:intron | 30.0% | |
| TAAGTACTCTCTTGATTTCA+AGG | + | chr8.4:14025805-14025824 | None:intergenic | 30.0% | |
| TTGTTCAATGAAGTGAATTG+CGG | + | chr8.4:14025375-14025394 | None:intergenic | 30.0% | |
| ! | ATGATTCCATCCTTGTTTAT+AGG | - | chr8.4:14026267-14026286 | MS.gene59071:intron | 30.0% |
| !! | GAAATCAAGAGAGTACTTAT+CGG | - | chr8.4:14025806-14025825 | MS.gene59071:intron | 30.0% |
| AAAGAGACAGTTCATTCCAA+AGG | + | chr8.4:14025933-14025952 | None:intergenic | 35.0% | |
| AGCCAGATCAATCGTATTTA+CGG | - | chr8.4:14026372-14026391 | MS.gene59071:CDS | 35.0% | |
| ATTTGTACCGAGTTCAGATT+TGG | - | chr8.4:14025531-14025550 | MS.gene59071:intron | 35.0% | |
| CATTCCAAAGGAACAATATC+CGG | + | chr8.4:14025921-14025940 | None:intergenic | 35.0% | |
| TAATAATTTGGAAGGCAGCA+GGG | - | chr8.4:14025832-14025851 | MS.gene59071:intron | 35.0% | |
| TCAATCGTATTTACGGTGAT+TGG | - | chr8.4:14026379-14026398 | MS.gene59071:CDS | 35.0% | |
| TGCAAAAATGTTCCTTCCTA+AGG | - | chr8.4:14026232-14026251 | MS.gene59071:CDS | 35.0% | |
| TGTTTCCAGAGCATAACAAA+AGG | + | chr8.4:14026417-14026436 | None:intergenic | 35.0% | |
| ! | AGGAACATTTTTGCAAGACA+AGG | + | chr8.4:14026227-14026246 | None:intergenic | 35.0% |
| ! | ATTTTTGCAAGACAAGGCAA+AGG | + | chr8.4:14026221-14026240 | None:intergenic | 35.0% |
| ! | TCATTTTCCATCAGAACCTT+AGG | + | chr8.4:14026251-14026270 | None:intergenic | 35.0% |
| ! | TTTTTGCAAGACAAGGCAAA+GGG | + | chr8.4:14026220-14026239 | None:intergenic | 35.0% |
| !! | AGATTTGGTGCTTGTAGAAA+CGG | - | chr8.4:14025546-14025565 | MS.gene59071:intron | 35.0% |
| !! | TGATTTGTTTGATTGAGGCA+AGG | + | chr8.4:14025487-14025506 | None:intergenic | 35.0% |
| !! | TGCTCTGATTTGTTTGATTG+AGG | + | chr8.4:14025492-14025511 | None:intergenic | 35.0% |
| !! | TGTGCAAGAAGTATTGCAAT+GGG | - | chr8.4:14025712-14025731 | MS.gene59071:intron | 35.0% |
| ACAAGCACCAAATCTGAACT+CGG | + | chr8.4:14025541-14025560 | None:intergenic | 40.0% | |
| ACATCAATGCATCAGAAGGA+GGG | + | chr8.4:14025446-14025465 | None:intergenic | 40.0% | |
| ATCATGGAGCTGAAGAGATA+AGG | - | chr8.4:14025586-14025605 | MS.gene59071:intron | 40.0% | |
| ATGAAGATCCTGCACAGTTT+AGG | - | chr8.4:14025772-14025791 | MS.gene59071:intron | 40.0% | |
| CACCGTAAATACGATTGATC+TGG | + | chr8.4:14026377-14026396 | None:intergenic | 40.0% | |
| CATACCGGATATTGTTCCTT+TGG | - | chr8.4:14025914-14025933 | MS.gene59071:CDS | 40.0% | |
| CTAATAATTTGGAAGGCAGC+AGG | - | chr8.4:14025831-14025850 | MS.gene59071:intron | 40.0% | |
| CTCTTTGCTCATGATGAAGA+AGG | - | chr8.4:14025947-14025966 | MS.gene59071:CDS | 40.0% | |
| TGAAGATCCTGCACAGTTTA+GGG | - | chr8.4:14025773-14025792 | MS.gene59071:intron | 40.0% | |
| TTCAATGAAGTGAATTGCGG+CGG | + | chr8.4:14025372-14025391 | None:intergenic | 40.0% | |
| TTTCCATCAGAACCTTAGGA+AGG | + | chr8.4:14026247-14026266 | None:intergenic | 40.0% | |
| ! | ACGGTGATTGGATTGATGAT+TGG | - | chr8.4:14026391-14026410 | MS.gene59071:CDS | 40.0% |
| ! | CTAAGAAGAAGCGTACACTT+GGG | - | chr8.4:14026340-14026359 | MS.gene59071:intron | 40.0% |
| ! | CTTCTTCCTTTTCCACAAGT+CGG | + | chr8.4:14026314-14026333 | None:intergenic | 40.0% |
| ! | TTCTTCCTTTTCCACAAGTC+GGG | + | chr8.4:14026313-14026332 | None:intergenic | 40.0% |
| !! | CTGTGCAAGAAGTATTGCAA+TGG | - | chr8.4:14025711-14025730 | MS.gene59071:intron | 40.0% |
| !! | GTGCAAGAAGTATTGCAATG+GGG | - | chr8.4:14025713-14025732 | MS.gene59071:intron | 40.0% |
| !!! | ATTGGCCTTTTGTTATGCTC+TGG | - | chr8.4:14026409-14026428 | MS.gene59071:CDS | 40.0% |
| AACGAAGATCAACTGCAGCT+GGG | - | chr8.4:14025648-14025667 | MS.gene59071:intron | 45.0% | |
| AAGGCAAAGGGAAAACCAGT+AGG | + | chr8.4:14026208-14026227 | None:intergenic | 45.0% | |
| ACCTGCTCTGCCTATAAACA+AGG | + | chr8.4:14026280-14026299 | None:intergenic | 45.0% | |
| ACTGTGCAGGATCTTCATGA+AGG | + | chr8.4:14025770-14025789 | None:intergenic | 45.0% | |
| AGACTAAGCTGTGTCGCAAT+TGG | - | chr8.4:14025888-14025907 | MS.gene59071:CDS | 45.0% | |
| ATGGAAAATGCTCCTCCTAC+TGG | - | chr8.4:14026190-14026209 | MS.gene59071:CDS | 45.0% | |
| CACATCAATGCATCAGAAGG+AGG | + | chr8.4:14025447-14025466 | None:intergenic | 45.0% | |
| CCAAATTTGCAGAAGCTAGG+TGG | - | chr8.4:14025618-14025637 | MS.gene59071:intron | 45.0% | |
| CCAAGTGTACGCTTCTTCTT+AGG | + | chr8.4:14026342-14026361 | None:intergenic | 45.0% | |
| CCACCAAATTTGCAGAAGCT+AGG | - | chr8.4:14025615-14025634 | MS.gene59071:intron | 45.0% | |
| CCACCTAGCTTCTGCAAATT+TGG | + | chr8.4:14025621-14025640 | None:intergenic | 45.0% | |
| CCTAGCTTCTGCAAATTTGG+TGG | + | chr8.4:14025618-14025637 | None:intergenic | 45.0% | |
| GAATCATCCCTAAACTGTGC+AGG | + | chr8.4:14025783-14025802 | None:intergenic | 45.0% | |
| GCTCTGCCTATAAACAAGGA+TGG | + | chr8.4:14026276-14026295 | None:intergenic | 45.0% | |
| GGTCACATCAATGCATCAGA+AGG | + | chr8.4:14025450-14025469 | None:intergenic | 45.0% | |
| TGCAGTTGATCTTCGTTGCA+CGG | + | chr8.4:14025645-14025664 | None:intergenic | 45.0% | |
| ! | AGCTTCTGCAAATTTGGTGG+TGG | + | chr8.4:14025615-14025634 | None:intergenic | 45.0% |
| ! | AGGAGGGCATATCAATGCAT+TGG | + | chr8.4:14025430-14025449 | None:intergenic | 45.0% |
| ! | ATGTACCCGACTTGTGGAAA+AGG | - | chr8.4:14026305-14026324 | MS.gene59071:intron | 45.0% |
| ! | CCTAAGAAGAAGCGTACACT+TGG | - | chr8.4:14026339-14026358 | MS.gene59071:intron | 45.0% |
| ! | CTCTGGAAACACACCTCATT+TGG | - | chr8.4:14026426-14026445 | MS.gene59071:CDS | 45.0% |
| ! | GTTCCTTCCTAAGGTTCTGA+TGG | - | chr8.4:14026241-14026260 | MS.gene59071:CDS | 45.0% |
| ! | TCCTTGTTTATAGGCAGAGC+AGG | - | chr8.4:14026276-14026295 | MS.gene59071:intron | 45.0% |
| ! | TGTCGCAATTGGAAGCATAC+CGG | - | chr8.4:14025899-14025918 | MS.gene59071:CDS | 45.0% |
| ! | TTCTGAATGTACCCGACTTG+TGG | - | chr8.4:14026299-14026318 | MS.gene59071:intron | 45.0% |
| !! | CAAGAAGTATTGCAATGGGG+AGG | - | chr8.4:14025716-14025735 | MS.gene59071:intron | 45.0% |
| CAACGAAGATCAACTGCAGC+TGG | - | chr8.4:14025647-14025666 | MS.gene59071:intron | 50.0% | |
| CATTGATGTGACCTCCTCCA+AGG | - | chr8.4:14025457-14025476 | MS.gene59071:CDS | 50.0% | |
| GCAAAGGGAAAACCAGTAGG+AGG | + | chr8.4:14026205-14026224 | None:intergenic | 50.0% | |
| GCTGTGAACAAGCAAGCAAG+GGG | - | chr8.4:14025854-14025873 | MS.gene59071:CDS | 50.0% | |
| GGCTGTGAACAAGCAAGCAA+GGG | - | chr8.4:14025853-14025872 | MS.gene59071:CDS | 50.0% | |
| !! | AGCAAGCAAGGGGCACTTAT+CGG | - | chr8.4:14025864-14025883 | MS.gene59071:CDS | 50.0% |
| GATGTGACCTCCTCCAAGGA+TGG | - | chr8.4:14025461-14025480 | MS.gene59071:CDS | 55.0% | |
| GATTGAGGCAAGGCCATCCT+TGG | + | chr8.4:14025477-14025496 | None:intergenic | 55.0% | |
| GGGCTGTGAACAAGCAAGCA+AGG | - | chr8.4:14025852-14025871 | MS.gene59071:CDS | 55.0% | |
| TGAGGCAAGGCCATCCTTGG+AGG | + | chr8.4:14025474-14025493 | None:intergenic | 60.0% | |
| GGCAAGGCCATCCTTGGAGG+AGG | + | chr8.4:14025471-14025490 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 14025363 | 14026452 | 14025363 | ID=MS.gene59071 |
| chr8.4 | mRNA | 14025363 | 14026452 | 14025363 | ID=MS.gene59071.t1;Parent=MS.gene59071 |
| chr8.4 | exon | 14026352 | 14026452 | 14026352 | ID=MS.gene59071.t1.exon1;Parent=MS.gene59071.t1 |
| chr8.4 | CDS | 14026352 | 14026452 | 14026352 | ID=cds.MS.gene59071.t1;Parent=MS.gene59071.t1 |
| chr8.4 | exon | 14026056 | 14026267 | 14026056 | ID=MS.gene59071.t1.exon2;Parent=MS.gene59071.t1 |
| chr8.4 | CDS | 14026056 | 14026267 | 14026056 | ID=cds.MS.gene59071.t1;Parent=MS.gene59071.t1 |
| chr8.4 | exon | 14025847 | 14025969 | 14025847 | ID=MS.gene59071.t1.exon3;Parent=MS.gene59071.t1 |
| chr8.4 | CDS | 14025847 | 14025969 | 14025847 | ID=cds.MS.gene59071.t1;Parent=MS.gene59071.t1 |
| chr8.4 | exon | 14025363 | 14025526 | 14025363 | ID=MS.gene59071.t1.exon4;Parent=MS.gene59071.t1 |
| chr8.4 | CDS | 14025363 | 14025526 | 14025363 | ID=cds.MS.gene59071.t1;Parent=MS.gene59071.t1 |
| Gene Sequence |
| Protein sequence |
