AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59075

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59075.t1 MTR_4g113860 86.025 322 40 2 1 318 1 321 0.0 570
MS.gene59075.t1 MTR_4g113970 59.160 262 82 6 1 245 1 254 1.69e-107 313
MS.gene59075.t1 MTR_4g113940 50.164 305 116 7 1 304 1 270 1.28e-95 284
MS.gene59075.t1 MTR_3g050390 47.619 273 99 10 51 317 7 241 4.99e-74 228
MS.gene59075.t1 MTR_4g113890 41.914 303 103 6 1 303 1 230 1.69e-72 224
MS.gene59075.t1 MTR_8g066730 29.836 305 124 10 77 304 83 374 4.08e-27 109
MS.gene59075.t1 MTR_8g066730 31.163 215 88 5 77 241 83 287 6.06e-21 91.3
MS.gene59075.t1 MTR_3g466460 59.524 84 31 2 230 312 21 102 2.96e-19 82.8
MS.gene59075.t1 MTR_4g113980 60.870 69 23 3 238 304 24 90 2.23e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59075.t1 AT3G19360 37.812 320 136 9 49 307 59 376 4.44e-62 202
MS.gene59075.t1 AT3G19360 37.812 320 136 9 49 307 59 376 4.44e-62 202
MS.gene59075.t1 AT1G32360 26.280 293 146 4 82 304 90 382 2.00e-21 94.0
MS.gene59075.t1 AT2G35430 26.702 191 109 5 139 304 66 250 9.20e-13 67.4

Find 84 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGATCAATCGTGTTTA+TGG 0.098083 8.4:-14079578 MS.gene59075:CDS
TCCTTTGCCACAAGTCTTGC+AGG 0.252708 8.4:+14079644 None:intergenic
GACAGCAGCAGCTGAGAAAT+TGG 0.269527 8.4:-14081533 MS.gene59075:CDS
ATTGGTTCTTCAAAGTCATT+TGG 0.270173 8.4:-14081345 MS.gene59075:CDS
ATGAGGATCCTGCTAAGTTT+AGG 0.284721 8.4:-14081416 MS.gene59075:CDS
TCTATAAGCATTGGGGCTAT+TGG 0.301858 8.4:-14081363 MS.gene59075:CDS
TAAGACTGCCACCACGAAAT+TGG 0.308491 8.4:-14081587 MS.gene59075:CDS
TTGGTTCTTCAAAGTCATTT+GGG 0.308872 8.4:-14081344 MS.gene59075:CDS
GATGGAGGAAGAACCATCTT+TGG 0.330810 8.4:+14081717 None:intergenic
TGAGGATCCTGCTAAGTTTA+GGG 0.331108 8.4:-14081415 MS.gene59075:CDS
TCCACTGCACTTGGCAAGTT+GGG 0.333885 8.4:+14079524 None:intergenic
AATGGTGAGAAATGTTTCTT+TGG 0.334123 8.4:-14081459 MS.gene59075:CDS
TTGCGGCGGTGACATCTTAC+AGG 0.346131 8.4:+14081896 None:intergenic
AGCCAGCTAGAGGCACTTAT+TGG 0.353667 8.4:-14081311 MS.gene59075:CDS
ACTTCACGAAAAGCCTTAAC+AGG 0.358557 8.4:-14079701 MS.gene59075:CDS
TCAATCGTGTTTATGGTGAT+TGG 0.369324 8.4:-14079571 MS.gene59075:CDS
ATCAAGGGAGATGTCCCTTT+GGG 0.372752 8.4:-14081260 MS.gene59075:CDS
GCTAAGTTTAGGGATCATTC+TGG 0.384575 8.4:-14081405 MS.gene59075:CDS
TTCAAAGTCATTTGGGGCTG+TGG 0.389237 8.4:-14081337 MS.gene59075:CDS
ACGTGGTCTGCTCTGTTTGA+TGG 0.398666 8.4:+14081699 None:intergenic
ATCAACCCCATTCCTTCAAA+AGG 0.402198 8.4:-14081788 MS.gene59075:CDS
AGTGTTCTTCTATAAGCATT+GGG 0.403399 8.4:-14081371 MS.gene59075:CDS
AAAGAAACATTTCTCACCAT+TGG 0.440730 8.4:+14081461 None:intergenic
TTACAGGTTGGAATATTGTC+TGG 0.446583 8.4:+14081912 None:intergenic
TTAAGAAGATGCATTTACTT+TGG 0.454114 8.4:-14079610 MS.gene59075:CDS
CTCCACTGCACTTGGCAAGT+TGG 0.458746 8.4:+14079523 None:intergenic
ATCATTGGCAGAACCTGTTA+AGG 0.460853 8.4:+14079688 None:intergenic
CATCAAGGGAGATGTCCCTT+TGG 0.465946 8.4:-14081261 MS.gene59075:CDS
TGCCACAAGTCTTGCAGGGA+AGG 0.471441 8.4:+14079649 None:intergenic
ACAGCAGCAGCTGAGAAATT+GGG 0.480050 8.4:-14081532 MS.gene59075:CDS
CCCTGCAAGACTTGTGGCAA+AGG 0.480562 8.4:-14079645 MS.gene59075:CDS
GGTAGGTGCATATGCATCAT+TGG 0.482923 8.4:+14079673 None:intergenic
GATATGCCCTCCTCCAAAGA+TGG 0.484001 8.4:-14081730 MS.gene59075:CDS
CTAAGTTTAGGGATCATTCT+GGG 0.485241 8.4:-14081404 MS.gene59075:CDS
AAAGATTGCAACTTTGCTCA+TGG 0.488168 8.4:-14081621 MS.gene59075:CDS
GTTTCTACAAGTACCAAGTC+TGG 0.493005 8.4:+14081647 None:intergenic
AAGATTGCAACTTTGCTCAT+GGG 0.501520 8.4:-14081620 MS.gene59075:CDS
TTCCAATAAGTGCCTCTAGC+TGG 0.502818 8.4:+14081309 None:intergenic
AGTCAGTTCTCCACTGCACT+TGG 0.503719 8.4:+14079515 None:intergenic
TTCACAAGATGAAGCTATGC+AGG 0.506731 8.4:-14081491 MS.gene59075:CDS
CAATTTGCTCATGGAGAAGC+AGG 0.507401 8.4:-14081228 MS.gene59075:intron
ATCTCCCTTGATGCTGCCAA+TGG 0.509822 8.4:+14081271 None:intergenic
AGACTAAGACGTGTTTCCAT+TGG 0.510007 8.4:-14081287 MS.gene59075:CDS
GAATGATCCCTAAACTTAGC+AGG 0.515278 8.4:+14081408 None:intergenic
GAGTGTTCTTCTATAAGCAT+TGG 0.515583 8.4:-14081372 MS.gene59075:CDS
GTTTCCATTGGCAGCATCAA+GGG 0.516368 8.4:-14081275 MS.gene59075:CDS
AGACTTGGTACTTGTAGAAA+CGG 0.521320 8.4:-14081645 MS.gene59075:CDS
GAGGACTGTCAATTTGCTCA+TGG 0.526443 8.4:-14081237 MS.gene59075:CDS
AAGACTTGTGGCAAAGGAAG+AGG 0.529531 8.4:-14079639 MS.gene59075:CDS
TGTTTCCATTGGCAGCATCA+AGG 0.535568 8.4:-14081276 MS.gene59075:CDS
TGTGGCAAAGGAAGAGGAGC+AGG 0.535571 8.4:-14079633 MS.gene59075:CDS
ACTAGCTCCTGCCAATTTCG+TGG 0.541649 8.4:+14081576 None:intergenic
TGGTTCTTCAAAGTCATTTG+GGG 0.545756 8.4:-14081343 MS.gene59075:CDS
CCTTTGCCACAAGTCTTGCA+GGG 0.547135 8.4:+14079645 None:intergenic
AGATTGCAACTTTGCTCATG+GGG 0.548831 8.4:-14081619 MS.gene59075:CDS
TCCCAACTTGCCAAGTGCAG+TGG 0.549943 8.4:-14079525 MS.gene59075:CDS
AGGAGGGCATATCAAAGCAT+TGG 0.555491 8.4:+14081740 None:intergenic
ACTGCCACCACGAAATTGGC+AGG 0.556769 8.4:-14081583 MS.gene59075:CDS
GGAAGAACCATCTTTGGAGG+AGG 0.559899 8.4:+14081723 None:intergenic
AATTGACAGTCCTCCCCAAA+GGG 0.560613 8.4:+14081246 None:intergenic
TCAACCCCATTCCTTCAAAA+GGG 0.563397 8.4:-14081787 MS.gene59075:CDS
GGAGGAAGAACCATCTTTGG+AGG 0.566069 8.4:+14081720 None:intergenic
CACCTTCCCTGCAAGACTTG+TGG 0.570439 8.4:-14079651 MS.gene59075:CDS
GTCTGTAATAAGTCCAGACT+TGG 0.573982 8.4:-14081660 MS.gene59075:CDS
CACAAGTCTTGCAGGGAAGG+TGG 0.574444 8.4:+14079652 None:intergenic
AGTCTTGCAGGGAAGGTGGT+AGG 0.597135 8.4:+14079656 None:intergenic
GGCGGTGACATCTTACAGGT+TGG 0.597501 8.4:+14081900 None:intergenic
GTTAGTCCTCGCAGCAGCGA+CGG 0.605011 8.4:-14081555 MS.gene59075:CDS
GGCAAGTTGGGAAGAACAAG+AGG 0.612688 8.4:+14079536 None:intergenic
GCTGTGGACAAGCCAGCTAG+AGG 0.623188 8.4:-14081321 MS.gene59075:CDS
AAATTGACAGTCCTCCCCAA+AGG 0.626382 8.4:+14081245 None:intergenic
CTGTTTGATGTAGTGAATTG+CGG 0.628943 8.4:+14081879 None:intergenic
GAAGAACCATCTTTGGAGGA+GGG 0.630074 8.4:+14081724 None:intergenic
TGCTGTCCGTCGCTGCTGCG+AGG 0.631311 8.4:+14081549 None:intergenic
CACGAGTCTTGTAGAAAACG+TGG 0.640632 8.4:+14081682 None:intergenic
AGGGAGATGTCCCTTTGGGG+AGG 0.652421 8.4:-14081256 MS.gene59075:CDS
CTATGCAGGATGTATTCCAA+TGG 0.653635 8.4:-14081477 MS.gene59075:CDS
GTGTTCTTCTATAAGCATTG+GGG 0.654358 8.4:-14081370 MS.gene59075:CDS
TGGTCTGCTCTGTTTGATGG+AGG 0.659565 8.4:+14081702 None:intergenic
TCAAGGGAGATGTCCCTTTG+GGG 0.666746 8.4:-14081259 MS.gene59075:CDS
AGCTCCTGCCAATTTCGTGG+TGG 0.669372 8.4:+14081579 None:intergenic
GCAGCAGCTGAGAAATTGGG+AGG 0.680326 8.4:-14081529 MS.gene59075:CDS
TTTGATGTAGTGAATTGCGG+CGG 0.699237 8.4:+14081882 None:intergenic
AATATTGTCTGGAAGAACCA+TGG 0.708668 8.4:+14081923 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCAATATATATTAAAAAA+AGG + chr8.4:14081569-14081588 None:intergenic 10.0%
!! AAAAGATGAATATAACATAA+TGG + chr8.4:14081326-14081345 None:intergenic 15.0%
!! CAAAATCAATAATCAAAATA+GGG + chr8.4:14080271-14080290 None:intergenic 15.0%
!!! ATCCATTTACAAATTTTTTA+TGG + chr8.4:14080821-14080840 None:intergenic 15.0%
!!! TTAATCATCTCAATTTTATA+AGG - chr8.4:14081039-14081058 MS.gene59075:intron 15.0%
!!! TTTTAACAATTTAAACTGTT+TGG - chr8.4:14081642-14081661 MS.gene59075:CDS 15.0%
!! AATAATCAAAATAGGGAAAA+GGG + chr8.4:14080264-14080283 None:intergenic 20.0%
!! AGCCATAAAAAATTTGTAAA+TGG - chr8.4:14080816-14080835 MS.gene59075:intron 20.0%
!! ATATGATGTTCAAATTATAG+TGG - chr8.4:14080385-14080404 MS.gene59075:intron 20.0%
!! CCAAAATCAATAATCAAAAT+AGG + chr8.4:14080272-14080291 None:intergenic 20.0%
!!! CCTATTTTGATTATTGATTT+TGG - chr8.4:14080269-14080288 MS.gene59075:intron 20.0%
!!! GTGTTTATATGTTGTATTTT+TGG - chr8.4:14080874-14080893 MS.gene59075:intron 20.0%
!!! TATTTTTATGTATTGGATCA+CGG + chr8.4:14081282-14081301 None:intergenic 20.0%
!!! TCACATGTATTTTTATGTAT+TGG + chr8.4:14081289-14081308 None:intergenic 20.0%
! AAAACTGTGTTGTCAAATAT+CGG - chr8.4:14080495-14080514 MS.gene59075:intron 25.0%
! AAAGATAAAGTAATCACTAG+TGG + chr8.4:14080661-14080680 None:intergenic 25.0%
! AAGGCATGATTACTATTAAA+TGG - chr8.4:14081058-14081077 MS.gene59075:intron 25.0%
! CAATAATCAAAATAGGGAAA+AGG + chr8.4:14080265-14080284 None:intergenic 25.0%
! CATATTTGACTGCAATATTA+AGG + chr8.4:14081478-14081497 None:intergenic 25.0%
! CTTAATATTGCAGTCAAATA+TGG - chr8.4:14081476-14081495 MS.gene59075:CDS 25.0%
! TCAAATAGAAATTCCTAATG+TGG + chr8.4:14080987-14081006 None:intergenic 25.0%
! TCCAATGAAAAATGAAAACT+TGG - chr8.4:14079609-14079628 MS.gene59075:CDS 25.0%
! TGAGTTAGACATCAAATTAT+AGG + chr8.4:14081395-14081414 None:intergenic 25.0%
! TTAAGAAGATGCATTTACTT+TGG - chr8.4:14081828-14081847 MS.gene59075:CDS 25.0%
!! TACATATCAGTGTTGTTAAA+TGG - chr8.4:14080754-14080773 MS.gene59075:intron 25.0%
!! TATATGTCGATGGATATTTT+GGG - chr8.4:14081249-14081268 MS.gene59075:CDS 25.0%
!!! TAAGTGTTATTTTCTTCATG+AGG - chr8.4:14080005-14080024 MS.gene59075:intron 25.0%
!!! TCCAAGTTTTCATTTTTCAT+TGG + chr8.4:14079613-14079632 None:intergenic 25.0%
!!! TGACTGAAATTTTAGTAGTT+TGG - chr8.4:14081105-14081124 MS.gene59075:intron 25.0%
AAAGAAACATTTCTCACCAT+TGG + chr8.4:14079980-14079999 None:intergenic 30.0%
AACAAGATCAATCGTGTTTA+TGG - chr8.4:14081860-14081879 MS.gene59075:CDS 30.0%
AATGGTGAGAAATGTTTCTT+TGG - chr8.4:14079979-14079998 MS.gene59075:intron 30.0%
ACAACTGCTATGTTTATCTA+AGG - chr8.4:14080729-14080748 MS.gene59075:intron 30.0%
AGTGTTCTTCTATAAGCATT+GGG - chr8.4:14080067-14080086 MS.gene59075:intron 30.0%
ATCATATAATTCTGCTGTAC+TGG - chr8.4:14080296-14080315 MS.gene59075:intron 30.0%
CAATATTAAGGATTGTGACA+TGG + chr8.4:14081466-14081485 None:intergenic 30.0%
CTTGTCGGATAAAAAAAAGT+AGG - chr8.4:14081212-14081231 MS.gene59075:intron 30.0%
CTTTATAGCTGATAACAGAA+AGG + chr8.4:14081150-14081169 None:intergenic 30.0%
TGTTATCAGCTATAAAGCAT+GGG - chr8.4:14081153-14081172 MS.gene59075:intron 30.0%
! ATTGGTTCTTCAAAGTCATT+TGG - chr8.4:14080093-14080112 MS.gene59075:intron 30.0%
! GCGTATGATATTTTCATGTT+TGG - chr8.4:14080433-14080452 MS.gene59075:intron 30.0%
! GTATATGTCGATGGATATTT+TGG - chr8.4:14081248-14081267 MS.gene59075:CDS 30.0%
! TTGGTTCTTCAAAGTCATTT+GGG - chr8.4:14080094-14080113 MS.gene59075:intron 30.0%
!! CTAACTCATCAATAGTTTTG+AGG - chr8.4:14081407-14081426 MS.gene59075:CDS 30.0%
!! TCATTTTTCATTGGAAATCG+AGG + chr8.4:14079604-14079623 None:intergenic 30.0%
AAAGATTGCAACTTTGCTCA+TGG - chr8.4:14079817-14079836 MS.gene59075:intron 35.0%
AAGATTGCAACTTTGCTCAT+GGG - chr8.4:14079818-14079837 MS.gene59075:intron 35.0%
AGGAAAAGGAAAAACAGGTT+TGG + chr8.4:14081349-14081368 None:intergenic 35.0%
ATGAGTCAGAATACGATACA+TGG + chr8.4:14081671-14081690 None:intergenic 35.0%
CCAAAAGGAAAAGGAAAAAC+AGG + chr8.4:14081354-14081373 None:intergenic 35.0%
CTAAGTTTAGGGATCATTCT+GGG - chr8.4:14080034-14080053 MS.gene59075:intron 35.0%
CTGTTATCAGCTATAAAGCA+TGG - chr8.4:14081152-14081171 MS.gene59075:intron 35.0%
CTGTTTGATGTAGTGAATTG+CGG + chr8.4:14079562-14079581 None:intergenic 35.0%
GAGTGTTCTTCTATAAGCAT+TGG - chr8.4:14080066-14080085 MS.gene59075:intron 35.0%
GGATCGTTAAATATACTGGA+AGG + chr8.4:14080900-14080919 None:intergenic 35.0%
GTATATTTAACGATCCTCCA+TGG - chr8.4:14080904-14080923 MS.gene59075:intron 35.0%
TAAACCAGTGTTAACGATGA+CGG + chr8.4:14080627-14080646 None:intergenic 35.0%
TCAATCGTGTTTATGGTGAT+TGG - chr8.4:14081867-14081886 MS.gene59075:CDS 35.0%
TCTTTGTGTTCAAATTTCCG+TGG - chr8.4:14080676-14080695 MS.gene59075:intron 35.0%
TGGAGGATCGTTAAATATAC+TGG + chr8.4:14080904-14080923 None:intergenic 35.0%
TGGTTCTTCAAAGTCATTTG+GGG - chr8.4:14080095-14080114 MS.gene59075:intron 35.0%
! AGAAATTCTTGCCCTTTTGA+AGG + chr8.4:14079665-14079684 None:intergenic 35.0%
! AGACTTGGTACTTGTAGAAA+CGG - chr8.4:14079793-14079812 MS.gene59075:intron 35.0%
! ATATCAATGTGTTGCTACCA+CGG + chr8.4:14080696-14080715 None:intergenic 35.0%
! ATTTTCGTGTCAACCACATT+AGG - chr8.4:14080971-14080990 MS.gene59075:intron 35.0%
! GTGTTCTTCTATAAGCATTG+GGG - chr8.4:14080068-14080087 MS.gene59075:intron 35.0%
! TTACAGGTTGGAATATTGTC+TGG + chr8.4:14079529-14079548 None:intergenic 35.0%
!! CCTGTTTTTCCTTTTCCTTT+TGG - chr8.4:14081351-14081370 MS.gene59075:CDS 35.0%
!! TAGGTATCGAAATTGCTTGT+CGG - chr8.4:14081197-14081216 MS.gene59075:intron 35.0%
AAAAGTAGGATGCTGCACTT+TGG - chr8.4:14081226-14081245 MS.gene59075:intron 40.0%
AAACACACCATTGTGACCTA+TGG + chr8.4:14080860-14080879 None:intergenic 40.0%
ACTGTGTTGTCAAATATCGG+CGG - chr8.4:14080498-14080517 MS.gene59075:intron 40.0%
ACTTCACGAAAAGCCTTAAC+AGG - chr8.4:14081737-14081756 MS.gene59075:CDS 40.0%
AGACTAAGACGTGTTTCCAT+TGG - chr8.4:14080151-14080170 MS.gene59075:intron 40.0%
AGATTGCAACTTTGCTCATG+GGG - chr8.4:14079819-14079838 MS.gene59075:intron 40.0%
ATCAACCCCATTCCTTCAAA+AGG - chr8.4:14079650-14079669 MS.gene59075:CDS 40.0%
ATCATTGGCAGAACCTGTTA+AGG + chr8.4:14081753-14081772 None:intergenic 40.0%
ATGAGGATCCTGCTAAGTTT+AGG - chr8.4:14080022-14080041 MS.gene59075:intron 40.0%
CAGCAGAAACCAAAAGGAAA+AGG + chr8.4:14081363-14081382 None:intergenic 40.0%
CATAGCCATCCTTCACAAAT+TGG + chr8.4:14080555-14080574 None:intergenic 40.0%
CTATGCAGGATGTATTCCAA+TGG - chr8.4:14079961-14079980 MS.gene59075:intron 40.0%
GAATGATCCCTAAACTTAGC+AGG + chr8.4:14080033-14080052 None:intergenic 40.0%
GCTAAGTTTAGGGATCATTC+TGG - chr8.4:14080033-14080052 MS.gene59075:intron 40.0%
GTCTGTAATAAGTCCAGACT+TGG - chr8.4:14079778-14079797 MS.gene59075:intron 40.0%
GTTTCTACAAGTACCAAGTC+TGG + chr8.4:14079794-14079813 None:intergenic 40.0%
TCAACCCCATTCCTTCAAAA+GGG - chr8.4:14079651-14079670 MS.gene59075:CDS 40.0%
TGAGGATCCTGCTAAGTTTA+GGG - chr8.4:14080023-14080042 MS.gene59075:intron 40.0%
TGCACTTTGGTATATGTCGA+TGG - chr8.4:14081239-14081258 MS.gene59075:CDS 40.0%
TTCACAAGATGAAGCTATGC+AGG - chr8.4:14079947-14079966 MS.gene59075:intron 40.0%
TTTGATGTAGTGAATTGCGG+CGG + chr8.4:14079559-14079578 None:intergenic 40.0%
! CGGTCAAATGGCAGATTTTT+AGG - chr8.4:14080518-14080537 MS.gene59075:intron 40.0%
! GTTAAGGCTTTTCGTGAAGT+TGG + chr8.4:14081737-14081756 None:intergenic 40.0%
!! TCTATAAGCATTGGGGCTAT+TGG - chr8.4:14080075-14080094 MS.gene59075:intron 40.0%
!! TCTTGCCCTTTTGAAGGAAT+GGG + chr8.4:14079659-14079678 None:intergenic 40.0%
!! TTCTTGCCCTTTTGAAGGAA+TGG + chr8.4:14079660-14079679 None:intergenic 40.0%
!!! TCCTTTTGGTTTCTGCTGAT+GGG - chr8.4:14081365-14081384 MS.gene59075:CDS 40.0%
!!! TTCCTTTTGGTTTCTGCTGA+TGG - chr8.4:14081364-14081383 MS.gene59075:CDS 40.0%
AAATTGACAGTCCTCCCCAA+AGG + chr8.4:14080196-14080215 None:intergenic 45.0%
AAGACTTGTGGCAAAGGAAG+AGG - chr8.4:14081799-14081818 MS.gene59075:CDS 45.0%
AATTGACAGTCCTCCCCAAA+GGG + chr8.4:14080195-14080214 None:intergenic 45.0%
ACAGCAGCAGCTGAGAAATT+GGG - chr8.4:14079906-14079925 MS.gene59075:intron 45.0%
ACCCATCAGCAGAAACCAAA+AGG + chr8.4:14081369-14081388 None:intergenic 45.0%
AGACGTCTATGCAACAATCG+TGG - chr8.4:14081536-14081555 MS.gene59075:CDS 45.0%
ATCAAGGGAGATGTCCCTTT+GGG - chr8.4:14080178-14080197 MS.gene59075:intron 45.0%
CATCCTTCACAAATTGGCCA+TGG + chr8.4:14080549-14080568 None:intergenic 45.0%
CATGGCCAATTTGTGAAGGA+TGG - chr8.4:14080547-14080566 MS.gene59075:intron 45.0%
GATGGAGGAAGAACCATCTT+TGG + chr8.4:14079724-14079743 None:intergenic 45.0%
GGTAGGTGCATATGCATCAT+TGG + chr8.4:14081768-14081787 None:intergenic 45.0%
GTCAAATATCGGCGGTCAAA+TGG - chr8.4:14080506-14080525 MS.gene59075:intron 45.0%
GTTTCCATTGGCAGCATCAA+GGG - chr8.4:14080163-14080182 MS.gene59075:intron 45.0%
TAAGACTGCCACCACGAAAT+TGG - chr8.4:14079851-14079870 MS.gene59075:intron 45.0%
TAAGCCACCATAAAACGCCA+TGG + chr8.4:14080578-14080597 None:intergenic 45.0%
TCTGCCACACTATTCTGTCT+TGG + chr8.4:14080785-14080804 None:intergenic 45.0%
TGTTTCCATTGGCAGCATCA+AGG - chr8.4:14080162-14080181 MS.gene59075:intron 45.0%
TTCCAATAAGTGCCTCTAGC+TGG + chr8.4:14080132-14080151 None:intergenic 45.0%
! AGGAGGGCATATCAAAGCAT+TGG + chr8.4:14079701-14079720 None:intergenic 45.0%
! CAATTTGCTCATGGAGAAGC+AGG - chr8.4:14080210-14080229 MS.gene59075:intron 45.0%
! CACGAGTCTTGTAGAAAACG+TGG + chr8.4:14079759-14079778 None:intergenic 45.0%
! GAAGAACCATCTTTGGAGGA+GGG + chr8.4:14079717-14079736 None:intergenic 45.0%
! GAGGACTGTCAATTTGCTCA+TGG - chr8.4:14080201-14080220 MS.gene59075:intron 45.0%
! TATGCCATGGCGTTTTATGG+TGG - chr8.4:14080571-14080590 MS.gene59075:intron 45.0%
! TTCAAAGTCATTTGGGGCTG+TGG - chr8.4:14080101-14080120 MS.gene59075:intron 45.0%
!! CTTGCCCTTTTGAAGGAATG+GGG + chr8.4:14079658-14079677 None:intergenic 45.0%
!!! CAGATTTTTAGGAAGCGCCA+TGG - chr8.4:14080529-14080548 MS.gene59075:intron 45.0%
ACCTACTTGCGTAACCACCA+CGG + chr8.4:14081182-14081201 None:intergenic 50.0%
ACGTGGTCTGCTCTGTTTGA+TGG + chr8.4:14079742-14079761 None:intergenic 50.0%
ACTAGCTCCTGCCAATTTCG+TGG + chr8.4:14079865-14079884 None:intergenic 50.0%
AGCGCCAAGACAGAATAGTG+TGG - chr8.4:14080778-14080797 MS.gene59075:intron 50.0%
AGCGTCACCATAGGTCACAA+TGG - chr8.4:14080850-14080869 MS.gene59075:intron 50.0%
AGTGACAGTGTTGTCCATGG+AGG + chr8.4:14080921-14080940 None:intergenic 50.0%
ATCTCCCTTGATGCTGCCAA+TGG + chr8.4:14080170-14080189 None:intergenic 50.0%
CACAGTGACAGTGTTGTCCA+TGG + chr8.4:14080924-14080943 None:intergenic 50.0%
CATCAAGGGAGATGTCCCTT+TGG - chr8.4:14080177-14080196 MS.gene59075:intron 50.0%
CCATCCGTCATCGTTAACAC+TGG - chr8.4:14080620-14080639 MS.gene59075:intron 50.0%
CCTTTGCCACAAGTCTTGCA+GGG + chr8.4:14081796-14081815 None:intergenic 50.0%
CTATAAAGCATGGGTACCCG+TGG - chr8.4:14081162-14081181 MS.gene59075:intron 50.0%
GACAGCAGCAGCTGAGAAAT+TGG - chr8.4:14079905-14079924 MS.gene59075:intron 50.0%
GATATGCCCTCCTCCAAAGA+TGG - chr8.4:14079708-14079727 MS.gene59075:CDS 50.0%
GGAGGAAGAACCATCTTTGG+AGG + chr8.4:14079721-14079740 None:intergenic 50.0%
TCAAGGGAGATGTCCCTTTG+GGG - chr8.4:14080179-14080198 MS.gene59075:intron 50.0%
TCCACTGCACTTGGCAAGTT+GGG + chr8.4:14081917-14081936 None:intergenic 50.0%
TCCTTTGCCACAAGTCTTGC+AGG + chr8.4:14081797-14081816 None:intergenic 50.0%
TGGTCTGCTCTGTTTGATGG+AGG + chr8.4:14079739-14079758 None:intergenic 50.0%
TGTGAAGGATGGCTATGCCA+TGG - chr8.4:14080558-14080577 MS.gene59075:intron 50.0%
! GGAAGAACCATCTTTGGAGG+AGG + chr8.4:14079718-14079737 None:intergenic 50.0%
! GGCAAGTTGGGAAGAACAAG+AGG + chr8.4:14081905-14081924 None:intergenic 50.0%
! GGCTATGCCATGGCGTTTTA+TGG - chr8.4:14080568-14080587 MS.gene59075:intron 50.0%
!! AGCCAGCTAGAGGCACTTAT+TGG - chr8.4:14080127-14080146 MS.gene59075:intron 50.0%
!! CCAGTGTTAACGATGACGGA+TGG + chr8.4:14080623-14080642 None:intergenic 50.0%
!! GTGTTAACGATGACGGATGG+TGG + chr8.4:14080620-14080639 None:intergenic 50.0%
ACTGCCACCACGAAATTGGC+AGG - chr8.4:14079855-14079874 MS.gene59075:intron 55.0%
AGCTCCTGCCAATTTCGTGG+TGG + chr8.4:14079862-14079881 None:intergenic 55.0%
AGTCTTGCAGGGAAGGTGGT+AGG + chr8.4:14081785-14081804 None:intergenic 55.0%
CACAAGTCTTGCAGGGAAGG+TGG + chr8.4:14081789-14081808 None:intergenic 55.0%
CACCTTCCCTGCAAGACTTG+TGG - chr8.4:14081787-14081806 MS.gene59075:CDS 55.0%
CCCTGCAAGACTTGTGGCAA+AGG - chr8.4:14081793-14081812 MS.gene59075:CDS 55.0%
CCTACTTGCGTAACCACCAC+GGG + chr8.4:14081181-14081200 None:intergenic 55.0%
CTCCACTGCACTTGGCAAGT+TGG + chr8.4:14081918-14081937 None:intergenic 55.0%
GCAGCAGCTGAGAAATTGGG+AGG - chr8.4:14079909-14079928 MS.gene59075:intron 55.0%
GCGCCATGGCCAATTTGTGA+AGG - chr8.4:14080543-14080562 MS.gene59075:intron 55.0%
GGCGGTGACATCTTACAGGT+TGG + chr8.4:14079541-14079560 None:intergenic 55.0%
TAAAGCATGGGTACCCGTGG+TGG - chr8.4:14081165-14081184 MS.gene59075:intron 55.0%
TCCCAACTTGCCAAGTGCAG+TGG - chr8.4:14081913-14081932 MS.gene59075:CDS 55.0%
TGCAGCTGCAGTTGCAGTGT+CGG - chr8.4:14081503-14081522 MS.gene59075:CDS 55.0%
TGCCACAAGTCTTGCAGGGA+AGG + chr8.4:14081792-14081811 None:intergenic 55.0%
TGTGGCAAAGGAAGAGGAGC+AGG - chr8.4:14081805-14081824 MS.gene59075:CDS 55.0%
TTGCGGCGGTGACATCTTAC+AGG + chr8.4:14079545-14079564 None:intergenic 55.0%
! TGTGACCTATGGTGACGCTG+TGG + chr8.4:14080849-14080868 None:intergenic 55.0%
CCCGTGGTGGTTACGCAAGT+AGG - chr8.4:14081178-14081197 MS.gene59075:intron 60.0%
GCTGTGGACAAGCCAGCTAG+AGG - chr8.4:14080117-14080136 MS.gene59075:intron 60.0%
GTCTGCCACAGCGTCACCAT+AGG - chr8.4:14080841-14080860 MS.gene59075:intron 60.0%
GTTAGTCCTCGCAGCAGCGA+CGG - chr8.4:14079883-14079902 MS.gene59075:intron 60.0%
! AGGGAGATGTCCCTTTGGGG+AGG - chr8.4:14080182-14080201 MS.gene59075:intron 60.0%
TGCTGTCCGTCGCTGCTGCG+AGG + chr8.4:14079892-14079911 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 14079517 14081943 14079517 ID=MS.gene59075
chr8.4 mRNA 14079517 14081943 14079517 ID=MS.gene59075.t1;Parent=MS.gene59075
chr8.4 exon 14081229 14081943 14081229 ID=MS.gene59075.t1.exon1;Parent=MS.gene59075.t1
chr8.4 CDS 14081229 14081943 14081229 ID=cds.MS.gene59075.t1;Parent=MS.gene59075.t1
chr8.4 exon 14079517 14079758 14079517 ID=MS.gene59075.t1.exon2;Parent=MS.gene59075.t1
chr8.4 CDS 14079517 14079758 14079517 ID=cds.MS.gene59075.t1;Parent=MS.gene59075.t1
Gene Sequence

>MS.gene59075

ATGGTTCTTCCAGACAATATTCCAACCTGTAAGATGTCACCGCCGCAATTCACTACATCAAACAGCGATATCATTGAAGTGAGACCTCGATTTCCAATGAAAAATGAAAACTTGGAACAACATTCAACATTGTATCAACCCCATTCCTTCAAAAGGGCAAGAATTTCTGATAACAATCGTTCCAATGCTTTGATATGCCCTCCTCCAAAGATGGTTCTTCCTCCATCAAACAGAGCAGACCACGTTTTCTACAAGACTCGTGTCTGTAATAAGTCCAGACTTGGTACTTGTAGAAACGGTAAAGATTGCAACTTTGCTCATGGGGTTGAAGAACTAAGACTGCCACCACGAAATTGGCAGGAGCTAGTTAGTCCTCGCAGCAGCGACGGACAGCAGCAGCTGAGAAATTGGGAGGAAGATCAAAAAATGATTCACAAGATGAAGCTATGCAGGATGTATTCCAATGGTGAGAAATGTTTCTTTGGTAGTAAGTGTTATTTTCTTCATGAGGATCCTGCTAAGTTTAGGGATCATTCTGGGAAGTCAAGAGAGTGTTCTTCTATAAGCATTGGGGCTATTGGTTCTTCAAAGTCATTTGGGGCTGTGGACAAGCCAGCTAGAGGCACTTATTGGAAGACTAAGACGTGTTTCCATTGGCAGCATCAAGGGAGATGTCCCTTTGGGGAGGACTGTCAATTTGCTCATGGAGAAGCAGGTAATAATAACTCATCTTTGTGTTGATTTCCCTTTTCCCTATTTTGATTATTGATTTTGGTTTTATCATATAATTCTGCTGTACTGGTTTTGTTTTGTATAGAGAATATGTTTGTTGATTTTTTTGATGATGTGCAGAAGATCATTTTATGTTATATGATGTTCAAATTATAGTGGTTGAATGTATGTTATTTTCATTGCTGCGTATGATATTTTCATGTTTGGTATGATATTTTGTTCACACAACAAATGCTAATTCAGTTTAAAACTGTGTTGTCAAATATCGGCGGTCAAATGGCAGATTTTTAGGAAGCGCCATGGCCAATTTGTGAAGGATGGCTATGCCATGGCGTTTTATGGTGGCTTAAAATTACACATTTGATTTTCCACCATCCGTCATCGTTAACACTGGTTTAAAATTAATTTACCACTAGTGATTACTTTATCTTTGTGTTCAAATTTCCGTGGTAGCAACACATTGATATGTGAGATTTTGTGACAACTGCTATGTTTATCTAAGGTTTACATATCAGTGTTGTTAAATGGCAGCGCCAAGACAGAATAGTGTGGCAGATTTTTGACGTCAGCCATAAAAAATTTGTAAATGGATGTCTGCCACAGCGTCACCATAGGTCACAATGGTGTGTTTATATGTTGTATTTTTGGCCTTCCAGTATATTTAACGATCCTCCATGGACAACACTGTCACTGTGTTGCATATAACTTGAATGAAGTTCTGTATTTTCGTGTCAACCACATTAGGAATTTCTATTTGATAGAAATGTCTAGATTTCATAAATTAAAATAGTTAATCATCTCAATTTTATAAGGCATGATTACTATTAAATGGTGTTAAAAGTTTGTGTTTGTTACTTGACTGAAATTTTAGTAGTTTGGAGTAGTCTGCGAAAAATTTCCTTTCTGTTATCAGCTATAAAGCATGGGTACCCGTGGTGGTTACGCAAGTAGGTATCGAAATTGCTTGTCGGATAAAAAAAAGTAGGATGCTGCACTTTGGTATATGTCGATGGATATTTTGGGTATGCATCCGTGATCCAATACATAAAAATACATGTGATTTTGAACAATAAACCATTATGTTATATTCATCTTTTCCAAACCTGTTTTTCCTTTTCCTTTTGGTTTCTGCTGATGGGTGTGCCTATAATTTGATGTCTAACTCATCAATAGTTTTGAGGTACATTGCTCATTTGCAATGTTTTAAACTGCGACCATGTCACAATCCTTAATATTGCAGTCAAATATGGTTAATGCAGCTGCAGTTGCAGTGTCGGAGACTTGAAAAGACGTCTATGCAACAATCGTGGTCACTGTCCTTTTTTTAATATATATTGCTTGTTTGCGTGTGATTACTTGTATTTGCATATTGTGAACAAAAAGTACAAGATGATTTTAACAATTTAAACTGTTTGGTTTCCATGTATCGTATTCTGACTCATTGAAGCTGCAGTTGCAATAGCAAATTCAACAAAAGCTATCATTCCAACTTCACGAAAAGCCTTAACAGGTTCTGCCAATGATGCATATGCACCTACCACCTTCCCTGCAAGACTTGTGGCAAAGGAAGAGGAGCAGGTTAAGAAGATGCATTTACTTTGGTTGAAATTAAACAAGATCAATCGTGTTTATGGTGATTGGATTGATGATTCACCTCTTGTTCTTCCCAACTTGCCAAGTGCAGTGGAGAACTGA
Protein sequence

>MS.gene59075.t1

MVLPDNIPTCKMSPPQFTTSNSDIIEVRPRFPMKNENLEQHSTLYQPHSFKRARISDNNRSNALICPPPKMVLPPSNRADHVFYKTRVCNKSRLGTCRNGKDCNFAHGVEELRLPPRNWQELVSPRSSDGQQQLRNWEEDQKMIHKMKLCRMYSNGEKCFFGSKCYFLHEDPAKFRDHSGKSRECSSISIGAIGSSKSFGAVDKPARGTYWKTKTCFHWQHQGRCPFGEDCQFAHGEAVAIANSTKAIIPTSRKALTGSANDAYAPTTFPARLVAKEEEQVKKMHLLWLKLNKINRVYGDWIDDSPLVLPNLPSAVEN