AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59225

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59225.t1 MTR_1g098590 96.172 209 6 1 1 209 1 207 7.35e-108 309
MS.gene59225.t1 MTR_1g098590 96.172 209 6 1 1 209 1 207 2.40e-107 309
MS.gene59225.t1 MTR_1g098590 96.172 209 6 1 1 209 1 207 6.04e-107 308
MS.gene59225.t1 MTR_4g094390 38.333 180 57 5 44 209 36 175 5.35e-19 81.6
MS.gene59225.t1 MTR_3g010660 31.925 213 88 5 42 209 96 296 2.50e-17 79.3
MS.gene59225.t1 MTR_8g043960 33.493 209 106 6 28 209 85 287 1.57e-16 77.0
MS.gene59225.t1 MTR_5g088230 34.737 190 89 5 32 214 82 243 2.98e-15 72.8
MS.gene59225.t1 MTR_5g088230 35.135 185 85 5 32 209 87 243 4.10e-15 72.8
MS.gene59225.t1 MTR_3g101780 51.807 83 33 2 131 209 318 397 1.80e-14 72.0
MS.gene59225.t1 MTR_2g086390 33.168 202 104 8 23 205 62 251 3.65e-14 70.1
MS.gene59225.t1 MTR_2g086390 33.168 202 104 8 23 205 62 251 8.99e-14 68.9
MS.gene59225.t1 MTR_4g085910 54.839 62 24 1 148 209 289 346 1.79e-12 65.9
MS.gene59225.t1 MTR_7g088090 63.830 47 17 0 159 205 244 290 5.82e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59225.t1 AT5G44080 45.299 234 67 8 9 208 70 276 4.97e-49 163
MS.gene59225.t1 AT1G03970 48.469 196 69 7 19 208 63 232 5.02e-41 141
MS.gene59225.t1 AT1G45249 54.930 71 28 1 142 212 319 385 7.94e-15 73.2
MS.gene59225.t1 AT1G45249 49.425 87 40 1 126 212 318 400 8.95e-15 72.8
MS.gene59225.t1 AT1G45249 49.425 87 40 1 126 212 318 400 8.95e-15 72.8
MS.gene59225.t1 AT2G36270 50.000 92 43 2 126 217 321 409 4.89e-14 70.9
MS.gene59225.t1 AT2G36270 50.000 92 43 2 126 217 321 409 7.13e-14 70.1
MS.gene59225.t1 AT2G36270 50.000 92 43 2 126 217 321 409 7.13e-14 70.1
MS.gene59225.t1 AT2G41070 36.111 180 86 7 42 210 76 237 8.59e-14 68.6
MS.gene59225.t1 AT2G41070 34.270 178 90 6 42 209 85 245 2.16e-13 67.8
MS.gene59225.t1 AT2G41070 33.708 178 91 5 42 209 76 236 2.34e-13 67.8
MS.gene59225.t1 AT2G41070 33.708 178 91 5 42 209 76 236 2.34e-13 67.8
MS.gene59225.t1 AT2G41070 33.708 178 91 5 42 209 76 236 2.34e-13 67.8
MS.gene59225.t1 AT4G34000 59.259 54 22 0 159 212 368 421 1.41e-12 66.6
MS.gene59225.t1 AT3G56850 34.021 194 89 7 42 209 91 271 1.68e-12 65.5
MS.gene59225.t1 AT4G34000 54.237 59 27 0 159 217 368 426 4.84e-12 65.1
MS.gene59225.t1 AT4G34000 54.237 59 27 0 159 217 368 426 4.84e-12 65.1
MS.gene59225.t1 AT4G34000 54.237 59 27 0 159 217 368 426 4.84e-12 65.1
MS.gene59225.t1 AT1G45249 43.878 98 40 2 126 212 303 396 5.24e-12 64.7
MS.gene59225.t1 AT3G19290 57.627 59 25 0 151 209 339 397 6.41e-12 64.3
MS.gene59225.t1 AT3G19290 56.667 60 26 0 151 210 339 398 7.91e-12 64.3
MS.gene59225.t1 AT3G19290 56.667 60 26 0 151 210 339 398 7.91e-12 64.3
MS.gene59225.t1 AT3G19290 56.667 60 26 0 151 210 339 398 8.30e-12 64.3

Find 81 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATGACGATTCCGTTAT+CGG 0.332969 1.3:-67788005 MS.gene59225:CDS
TTATCCAATTGCTCCATAAC+AGG 0.338573 1.3:+67787824 None:intergenic
CAAGGAATTGAAAACGTTGA+TGG 0.345371 1.3:-67787938 MS.gene59225:CDS
GATTTGATCGTCGAGGAAAT+TGG 0.346078 1.3:+67788210 None:intergenic
AACGTTGATGGGTCGGTGAT+AGG 0.356163 1.3:-67787926 MS.gene59225:CDS
GATGGGTCGGTGATAGGGTT+TGG 0.356739 1.3:-67787920 MS.gene59225:CDS
CGGTGATAGGGTTTGGGAAT+GGG 0.358384 1.3:-67787913 MS.gene59225:CDS
GTGGAAATGGTTGAAGGTGG+GGG 0.368454 1.3:-67787866 MS.gene59225:CDS
CTCGAACCGTCGACGATGTC+TGG 0.371504 1.3:-67788162 MS.gene59225:CDS
ATGGGTCGGTGATAGGGTTT+GGG 0.380377 1.3:-67787919 MS.gene59225:CDS
TTAGGCTTATCAAGTGGAAT+TGG 0.384866 1.3:-67787496 MS.gene59225:intron
GAAGAGAGGACGGCCTGTTA+TGG 0.389128 1.3:-67787837 MS.gene59225:CDS
ATGGCAATGGGAATGTGAAT+GGG 0.390238 1.3:-67787889 MS.gene59225:CDS
GGGGTGGAAATGGTTGAAGG+TGG 0.394857 1.3:-67787869 MS.gene59225:CDS
TGTGCAGGCTGAGAGGAAAA+AGG 0.400339 1.3:-67786506 MS.gene59225:intron
GGTGGAAATGGTTGAAGGTG+GGG 0.410518 1.3:-67787867 MS.gene59225:CDS
GAGAATCTGCTGCTAGGTCT+AGG 0.414539 1.3:-67787763 MS.gene59225:CDS
TGAGGATGAGGAGGAGGGTG+AGG 0.414838 1.3:-67788032 MS.gene59225:CDS
TCGGTGATAGGGTTTGGGAA+TGG 0.430970 1.3:-67787914 MS.gene59225:CDS
GAATGTGAATGGGGTGGAAA+TGG 0.432712 1.3:-67787879 MS.gene59225:CDS
GCGGTGGTGAAGTGAGGTTT+AGG 0.434316 1.3:+67788232 None:intergenic
AATGGCAATGGGAATGTGAA+TGG 0.440487 1.3:-67787890 MS.gene59225:CDS
ACGGCCTGTTATGGAGCAAT+TGG 0.442422 1.3:-67787828 MS.gene59225:CDS
TTGGGAATGGGAATGGCAAT+GGG 0.446748 1.3:-67787901 MS.gene59225:CDS
TTTGGGAATGGGAATGGCAA+TGG 0.453383 1.3:-67787902 MS.gene59225:CDS
GCGATGTGATCTCCGGCGAC+AGG 0.458449 1.3:-67788123 MS.gene59225:CDS
CCACTGAGCGTCTCCGATAA+CGG 0.464675 1.3:+67787992 None:intergenic
TGCGGTTGAGGATGAGGAGG+AGG 0.464821 1.3:-67788038 MS.gene59225:CDS
TGGAAATGGTTGAAGGTGGG+GGG 0.467922 1.3:-67787865 MS.gene59225:CDS
ACGTTGATGGGTCGGTGATA+GGG 0.467935 1.3:-67787925 MS.gene59225:CDS
TCAAGTGGAATTGGAGTCCT+TGG 0.472571 1.3:-67787487 MS.gene59225:CDS
CGATGAGATGATGACGTTGG+AGG 0.475948 1.3:-67788080 MS.gene59225:CDS
AGATGGAGAAGAAGGTGGAT+CGG 0.476558 1.3:+67788255 None:intergenic
TTCTTTACACTCCCTGTCGC+CGG 0.477248 1.3:+67788111 None:intergenic
GGGTGGAAATGGTTGAAGGT+GGG 0.482957 1.3:-67787868 MS.gene59225:CDS
TGTGAAAGCTGGTGCGGTTG+AGG 0.485583 1.3:-67788050 MS.gene59225:CDS
GGTTTAGGAGATGGAGAAGA+AGG 0.490179 1.3:+67788247 None:intergenic
TGACAAGTTGATGAAGGAAA+AGG 0.495815 1.3:-67787442 MS.gene59225:CDS
GTGAAGTGAGGTTTAGGAGA+TGG 0.499875 1.3:+67788238 None:intergenic
TAGGTCTAGGGAACGCAAGC+AGG 0.503430 1.3:-67787750 MS.gene59225:intron
TCGAGGAGTGGCGACGGCGT+CGG 0.506745 1.3:+67788180 None:intergenic
GGAAAATGACAAGTTGATGA+AGG 0.507651 1.3:-67787448 MS.gene59225:CDS
AGCTGGTGCGGTTGAGGATG+AGG 0.510481 1.3:-67788044 MS.gene59225:CDS
ATAGGGTTTGGGAATGGGAA+TGG 0.514457 1.3:-67787908 MS.gene59225:CDS
AATGGGGTGGAAATGGTTGA+AGG 0.515608 1.3:-67787872 MS.gene59225:CDS
GAGTGGCGACGGCGTCGGCG+AGG 0.520677 1.3:+67788185 None:intergenic
ATCGGAAGAAGGCATGAGCT+TGG 0.521805 1.3:+67788273 None:intergenic
AAGGAATTGAAAACGTTGAT+GGG 0.523079 1.3:-67787937 MS.gene59225:CDS
TCGGAGACGCTCAGTGGTAG+TGG 0.524000 1.3:-67787986 MS.gene59225:CDS
GTCGCCGGCGATGTGATCTC+CGG 0.529993 1.3:-67788130 MS.gene59225:CDS
GAAGAAGGTGGATCGGAAGA+AGG 0.530586 1.3:+67788262 None:intergenic
TTAGGAGATGGAGAAGAAGG+TGG 0.530669 1.3:+67788250 None:intergenic
TGGCAATGGGAATGTGAATG+GGG 0.531390 1.3:-67787888 MS.gene59225:CDS
CTCCTCTAGCTTCACCGCCA+AGG 0.538630 1.3:+67787470 None:intergenic
AAATTGGCGGTGGTGAAGTG+AGG 0.542267 1.3:+67788226 None:intergenic
GCGGTTGAGGATGAGGAGGA+GGG 0.553341 1.3:-67788037 MS.gene59225:CDS
CATGAGCTTGGAAGAAGCCA+TGG 0.560742 1.3:+67788285 None:intergenic
AGAATCTGCTGCTAGGTCTA+GGG 0.563610 1.3:-67787762 MS.gene59225:CDS
CTCCAACGTCATCATCTCAT+CGG 0.569861 1.3:+67788081 None:intergenic
ATCGTCGAGGAAATTGGCGG+TGG 0.569885 1.3:+67788216 None:intergenic
AGAATAGAGAATCTGCTGCT+AGG 0.572500 1.3:-67787769 MS.gene59225:CDS
AGTGGCGACGGCGTCGGCGA+GGG 0.573280 1.3:+67788186 None:intergenic
ATCGTCGACGGTTCGAGGAG+TGG 0.573362 1.3:+67788168 None:intergenic
CAGACATCGTCGACGGTTCG+AGG 0.576404 1.3:+67788163 None:intergenic
GACGGTTCGAGGAGTGGCGA+CGG 0.580962 1.3:+67788174 None:intergenic
AATTGAAAACGTTGATGGGT+CGG 0.585668 1.3:-67787933 MS.gene59225:CDS
CGATGTGATCTCCGGCGACA+GGG 0.589289 1.3:-67788122 MS.gene59225:CDS
GTCTGGCGAGAGATCGTCGC+CGG 0.590254 1.3:-67788145 MS.gene59225:CDS
AAGCTGCTCAGCAGAGACAG+AGG 0.593315 1.3:-67787802 MS.gene59225:CDS
TTCCGATGAGATGATGACGT+TGG 0.607504 1.3:-67788083 MS.gene59225:CDS
TTGATCGTCGAGGAAATTGG+CGG 0.607652 1.3:+67788213 None:intergenic
CTCTCGCCAGACATCGTCGA+CGG 0.611545 1.3:+67788156 None:intergenic
CAATGGGAATGTGAATGGGG+TGG 0.612710 1.3:-67787885 MS.gene59225:CDS
GTCGCCGGAGATCACATCGC+CGG 0.615098 1.3:+67788126 None:intergenic
AGTGGAATTGGAGTCCTTGG+CGG 0.619638 1.3:-67787484 MS.gene59225:CDS
GTCCTTGGCGGTGAAGCTAG+AGG 0.622234 1.3:-67787472 MS.gene59225:CDS
CCGTTATCGGAGACGCTCAG+TGG 0.634758 1.3:-67787992 MS.gene59225:CDS
ATATTCTTGTGCAGGCTGAG+AGG 0.635094 1.3:-67786513 MS.gene59225:intron
TGGTGCGGTTGAGGATGAGG+AGG 0.665882 1.3:-67788041 MS.gene59225:CDS
CGAGGGAGATTTGATCGTCG+AGG 0.673387 1.3:+67788203 None:intergenic
CTTGGCGGTGAAGCTAGAGG+AGG 0.703301 1.3:-67787469 MS.gene59225:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGACATTTCATAAATT+AGG - chr1.3:67787682-67787701 MS.gene59225:intron 15.0%
!!! TTTTGACATTTCATAAATTA+GGG - chr1.3:67787683-67787702 MS.gene59225:intron 15.0%
!! ATTATAGTAAATTTCCGAAA+TGG - chr1.3:67787438-67787457 MS.gene59225:CDS 20.0%
!! CAAATATATAAAAACAAGCA+TGG + chr1.3:67788108-67788127 None:intergenic 20.0%
!! TCTAAATCTGAAAATTCAAA+GGG + chr1.3:67788193-67788212 None:intergenic 20.0%
!! TGTTTGAAAAAAAAAACAGA+AGG + chr1.3:67787939-67787958 None:intergenic 20.0%
!!! CATGCTTGTTTTTATATATT+TGG - chr1.3:67788106-67788125 MS.gene59225:CDS 20.0%
!!! GCTGATTTATAAAAAAATTG+CGG + chr1.3:67787371-67787390 None:intergenic 20.0%
!!! TATATATTTGGAATGTTTCT+TGG - chr1.3:67788118-67788137 MS.gene59225:CDS 20.0%
! AAACAGAAGGATTTCAATAA+TGG + chr1.3:67787926-67787945 None:intergenic 25.0%
! AATATTATCTGTTAGATGTC+AGG + chr1.3:67788240-67788259 None:intergenic 25.0%
! ATATTATCTGTTAGATGTCA+GGG + chr1.3:67788239-67788258 None:intergenic 25.0%
! CTCTAAATCTGAAAATTCAA+AGG + chr1.3:67788194-67788213 None:intergenic 25.0%
! TTCTAAACTATCATCAATAG+AGG + chr1.3:67787602-67787621 None:intergenic 25.0%
!! CACAAATAGAATGAGATTTT+AGG + chr1.3:67788042-67788061 None:intergenic 25.0%
!! CTCTATTTAAACTTTGTTGT+CGG - chr1.3:67787508-67787527 MS.gene59225:intron 25.0%
!!! TTTTGGTTTTGTGTGATTTT+AGG - chr1.3:67787254-67787273 MS.gene59225:intron 25.0%
AAAACTAATTGCACAGTCAA+AGG + chr1.3:67787980-67787999 None:intergenic 30.0%
AACAGATAATATTCTTGTGC+AGG - chr1.3:67788247-67788266 MS.gene59225:CDS 30.0%
AAGGAATTGAAAACGTTGAT+GGG - chr1.3:67786831-67786850 MS.gene59225:intron 30.0%
ATGTGTTAGGAGAACAAAAA+AGG + chr1.3:67788081-67788100 None:intergenic 30.0%
GAGATTGCTGTCAATTAATT+TGG - chr1.3:67787573-67787592 MS.gene59225:intron 30.0%
GTCTGATATGTTATGATACA+CGG - chr1.3:67787057-67787076 MS.gene59225:intron 30.0%
TATTATCTGTTAGATGTCAG+GGG + chr1.3:67788238-67788257 None:intergenic 30.0%
! ATTTAAACTTTGTTGTCGGT+AGG - chr1.3:67787512-67787531 MS.gene59225:intron 30.0%
! TAGGATTTAAAGTAGGGTTA+GGG - chr1.3:67787531-67787550 MS.gene59225:intron 30.0%
! TTTGGCAGTTTTCGATTTAA+GGG - chr1.3:67787656-67787675 MS.gene59225:intron 30.0%
!! AGGATTTCAATAATGGACAT+TGG + chr1.3:67787919-67787938 None:intergenic 30.0%
!!! TGCTTTTGTTTTTGTGATGT+GGG - chr1.3:67787218-67787237 MS.gene59225:intron 30.0%
!!! TGGGTTTTTGACTTTTGTTT+TGG - chr1.3:67787237-67787256 MS.gene59225:intron 30.0%
!!! TGTGCAATTAGTTTTTACGT+GGG - chr1.3:67787985-67788004 MS.gene59225:CDS 30.0%
!!! TTTTAGGACTGAATTCTATG+AGG + chr1.3:67788026-67788045 None:intergenic 30.0%
AACCATTCGACTCTGATTAA+GGG - chr1.3:67787841-67787860 MS.gene59225:CDS 35.0%
ACCATCATCAATTCCAAAGA+CGG + chr1.3:67787469-67787488 None:intergenic 35.0%
AGAACAAAAAAGGAACTTCG+TGG + chr1.3:67788071-67788090 None:intergenic 35.0%
ATTATCTGTTAGATGTCAGG+GGG + chr1.3:67788237-67788256 None:intergenic 35.0%
CAAGGAATTGAAAACGTTGA+TGG - chr1.3:67786830-67786849 MS.gene59225:intron 35.0%
TACAAGGCAAACATTCTTTG+TGG - chr1.3:67787803-67787822 MS.gene59225:CDS 35.0%
TACGTGGGTAATTTCCATAA+CGG - chr1.3:67788000-67788019 MS.gene59225:CDS 35.0%
TTAGGCTTATCAAGTGGAAT+TGG - chr1.3:67787272-67787291 MS.gene59225:intron 35.0%
TTATCCAATTGCTCCATAAC+AGG + chr1.3:67786947-67786966 None:intergenic 35.0%
! AATTGAAAACGTTGATGGGT+CGG - chr1.3:67786835-67786854 MS.gene59225:intron 35.0%
! CAAAGACGGTTTTACCATTT+CGG + chr1.3:67787455-67787474 None:intergenic 35.0%
! GTAGGATTTAAAGTAGGGTT+AGG - chr1.3:67787530-67787549 MS.gene59225:intron 35.0%
! GTGATTTTAGGCTTATCAAG+TGG - chr1.3:67787266-67787285 MS.gene59225:intron 35.0%
! GTTTGGCAGTTTTCGATTTA+AGG - chr1.3:67787655-67787674 MS.gene59225:intron 35.0%
! TGATGATTTTTCACGACAGA+GGG - chr1.3:67787624-67787643 MS.gene59225:intron 35.0%
! TGTCGGTAGGATTTAAAGTA+GGG - chr1.3:67787525-67787544 MS.gene59225:intron 35.0%
! TTGTCGGTAGGATTTAAAGT+AGG - chr1.3:67787524-67787543 MS.gene59225:intron 35.0%
!! GGAAAATGACAAGTTGATGA+AGG - chr1.3:67787320-67787339 MS.gene59225:intron 35.0%
!! TGACAAGTTGATGAAGGAAA+AGG - chr1.3:67787326-67787345 MS.gene59225:intron 35.0%
!!! CTGTGCAATTAGTTTTTACG+TGG - chr1.3:67787984-67788003 MS.gene59225:CDS 35.0%
!!! GTGCTTTTGTTTTTGTGATG+TGG - chr1.3:67787217-67787236 MS.gene59225:intron 35.0%
AATGGCAATGGGAATGTGAA+TGG - chr1.3:67786878-67786897 MS.gene59225:intron 40.0%
AGAATAGAGAATCTGCTGCT+AGG - chr1.3:67786999-67787018 MS.gene59225:intron 40.0%
ATGGCAATGGGAATGTGAAT+GGG - chr1.3:67786879-67786898 MS.gene59225:intron 40.0%
ATGTTATGATACACGGACAC+CGG - chr1.3:67787064-67787083 MS.gene59225:intron 40.0%
CAAATGTGTCAGTGTTGTGT+TGG - chr1.3:67787131-67787150 MS.gene59225:intron 40.0%
CGAAATGGTAAAACCGTCTT+TGG - chr1.3:67787453-67787472 MS.gene59225:CDS 40.0%
CTGAAAATTCAAAGGGTTGC+AGG + chr1.3:67788186-67788205 None:intergenic 40.0%
GAACCATTCGACTCTGATTA+AGG - chr1.3:67787840-67787859 MS.gene59225:CDS 40.0%
GAAGATGACGATTCCGTTAT+CGG - chr1.3:67786763-67786782 MS.gene59225:intron 40.0%
GATTTGATCGTCGAGGAAAT+TGG + chr1.3:67786561-67786580 None:intergenic 40.0%
GTTGGACTAAAAACACGTGT+CGG - chr1.3:67787149-67787168 MS.gene59225:intron 40.0%
TAAAAACACGTGTCGGATAC+AGG - chr1.3:67787156-67787175 MS.gene59225:intron 40.0%
TGAATTCTATGAGGCCGTTA+TGG + chr1.3:67788017-67788036 None:intergenic 40.0%
TTGCTGTGGTTAATGCTACA+AGG - chr1.3:67787787-67787806 MS.gene59225:CDS 40.0%
! GAGGATTTTCTTGTGAAAGC+TGG - chr1.3:67786707-67786726 MS.gene59225:intron 40.0%
! GTGATGATTTTTCACGACAG+AGG - chr1.3:67787623-67787642 MS.gene59225:intron 40.0%
! GTTGTGCTTGTTAACAGAAG+TGG - chr1.3:67787889-67787908 MS.gene59225:CDS 40.0%
! TTTTCTTGTGAAAGCTGGTG+CGG - chr1.3:67786712-67786731 MS.gene59225:intron 40.0%
!! AATGTTTCTTGGAGCACTGT+TGG - chr1.3:67788129-67788148 MS.gene59225:CDS 40.0%
!! ACCGTCTTTGGAATTGATGA+TGG - chr1.3:67787465-67787484 MS.gene59225:CDS 40.0%
!! TCGTTGGATTCGAGTTTTCA+AGG - chr1.3:67786812-67786831 MS.gene59225:intron 40.0%
!! TTGGAATTGATGATGGTCTG+TGG - chr1.3:67787472-67787491 MS.gene59225:CDS 40.0%
!!! TGGTAGTGGTATGTTTTCGT+TGG - chr1.3:67786796-67786815 MS.gene59225:intron 40.0%
AATGGGGTGGAAATGGTTGA+AGG - chr1.3:67786896-67786915 MS.gene59225:intron 45.0%
AGAATCTGCTGCTAGGTCTA+GGG - chr1.3:67787006-67787025 MS.gene59225:intron 45.0%
AGATGGAGAAGAAGGTGGAT+CGG + chr1.3:67786516-67786535 None:intergenic 45.0%
AGCAAAAAGCAAGCAGCTGA+TGG + chr1.3:67787772-67787791 None:intergenic 45.0%
ATAGGGTTTGGGAATGGGAA+TGG - chr1.3:67786860-67786879 MS.gene59225:intron 45.0%
ATATTCTTGTGCAGGCTGAG+AGG - chr1.3:67788255-67788274 MS.gene59225:CDS 45.0%
CAAGCAGCTGATGGAAAATG+TGG + chr1.3:67787763-67787782 None:intergenic 45.0%
CAAGCATGGTAGCATGTGTT+AGG + chr1.3:67788094-67788113 None:intergenic 45.0%
CTCCAACGTCATCATCTCAT+CGG + chr1.3:67786690-67786709 None:intergenic 45.0%
GAAAAAGGAGAGGTTCAAGC+AGG - chr1.3:67788277-67788296 MS.gene59225:CDS 45.0%
GAATGTGAATGGGGTGGAAA+TGG - chr1.3:67786889-67786908 MS.gene59225:intron 45.0%
GACAGAGGGACAAAGTTGTT+TGG - chr1.3:67787638-67787657 MS.gene59225:intron 45.0%
GGTTTAGGAGATGGAGAAGA+AGG + chr1.3:67786524-67786543 None:intergenic 45.0%
GTCCCTTAATCAGAGTCGAA+TGG + chr1.3:67787846-67787865 None:intergenic 45.0%
GTGAAGTGAGGTTTAGGAGA+TGG + chr1.3:67786533-67786552 None:intergenic 45.0%
TCAAGTGGAATTGGAGTCCT+TGG - chr1.3:67787281-67787300 MS.gene59225:intron 45.0%
TGGCAATGGGAATGTGAATG+GGG - chr1.3:67786880-67786899 MS.gene59225:intron 45.0%
TTAGGAGATGGAGAAGAAGG+TGG + chr1.3:67786521-67786540 None:intergenic 45.0%
TTGATCGTCGAGGAAATTGG+CGG + chr1.3:67786558-67786577 None:intergenic 45.0%
TTGGGAATGGGAATGGCAAT+GGG - chr1.3:67786867-67786886 MS.gene59225:intron 45.0%
TTTGGGAATGGGAATGGCAA+TGG - chr1.3:67786866-67786885 MS.gene59225:intron 45.0%
! TTCCGATGAGATGATGACGT+TGG - chr1.3:67786685-67786704 MS.gene59225:intron 45.0%
AAATTGGCGGTGGTGAAGTG+AGG + chr1.3:67786545-67786564 None:intergenic 50.0%
ACGGCCTGTTATGGAGCAAT+TGG - chr1.3:67786940-67786959 MS.gene59225:intron 50.0%
ACTCGTGTCAGTGTCATGTC+CGG + chr1.3:67787086-67787105 None:intergenic 50.0%
AGGCTGAGAGGAAAAAGGAG+AGG - chr1.3:67788267-67788286 MS.gene59225:CDS 50.0%
AGTGGAATTGGAGTCCTTGG+CGG - chr1.3:67787284-67787303 MS.gene59225:intron 50.0%
ATGGGTCGGTGATAGGGTTT+GGG - chr1.3:67786849-67786868 MS.gene59225:intron 50.0%
CAATGGGAATGTGAATGGGG+TGG - chr1.3:67786883-67786902 MS.gene59225:intron 50.0%
CGGTGATAGGGTTTGGGAAT+GGG - chr1.3:67786855-67786874 MS.gene59225:intron 50.0%
GAAGAAGGTGGATCGGAAGA+AGG + chr1.3:67786509-67786528 None:intergenic 50.0%
GAGAATCTGCTGCTAGGTCT+AGG - chr1.3:67787005-67787024 MS.gene59225:intron 50.0%
GGGTGGAAATGGTTGAAGGT+GGG - chr1.3:67786900-67786919 MS.gene59225:intron 50.0%
GGTGGAAATGGTTGAAGGTG+GGG - chr1.3:67786901-67786920 MS.gene59225:intron 50.0%
GTGGAAATGGTTGAAGGTGG+GGG - chr1.3:67786902-67786921 MS.gene59225:intron 50.0%
TCGGTGATAGGGTTTGGGAA+TGG - chr1.3:67786854-67786873 MS.gene59225:intron 50.0%
TGGAAATGGTTGAAGGTGGG+GGG - chr1.3:67786903-67786922 MS.gene59225:intron 50.0%
TGTGCAGGCTGAGAGGAAAA+AGG - chr1.3:67788262-67788281 MS.gene59225:CDS 50.0%
TTCTTTACACTCCCTGTCGC+CGG + chr1.3:67786660-67786679 None:intergenic 50.0%
! AACGTTGATGGGTCGGTGAT+AGG - chr1.3:67786842-67786861 MS.gene59225:intron 50.0%
! ACGTTGATGGGTCGGTGATA+GGG - chr1.3:67786843-67786862 MS.gene59225:intron 50.0%
! ATCGGAAGAAGGCATGAGCT+TGG + chr1.3:67786498-67786517 None:intergenic 50.0%
! CGATGAGATGATGACGTTGG+AGG - chr1.3:67786688-67786707 MS.gene59225:intron 50.0%
!!! CAGCTGCTTGCTTTTTGCTG+TGG - chr1.3:67787773-67787792 MS.gene59225:CDS 50.0%
AAATGGTTGAAGGTGGGGGG+AGG - chr1.3:67786906-67786925 MS.gene59225:intron 55.0%
AAGCTGCTCAGCAGAGACAG+AGG - chr1.3:67786966-67786985 MS.gene59225:intron 55.0%
AATGGTTGAAGGTGGGGGGA+GGG - chr1.3:67786907-67786926 MS.gene59225:intron 55.0%
ATCGTCGAGGAAATTGGCGG+TGG + chr1.3:67786555-67786574 None:intergenic 55.0%
CCACTGAGCGTCTCCGATAA+CGG + chr1.3:67786779-67786798 None:intergenic 55.0%
CGAGGGAGATTTGATCGTCG+AGG + chr1.3:67786568-67786587 None:intergenic 55.0%
GAAGAGAGGACGGCCTGTTA+TGG - chr1.3:67786931-67786950 MS.gene59225:intron 55.0%
GATGGGTCGGTGATAGGGTT+TGG - chr1.3:67786848-67786867 MS.gene59225:intron 55.0%
GCGGTGGTGAAGTGAGGTTT+AGG + chr1.3:67786539-67786558 None:intergenic 55.0%
GGGGTGGAAATGGTTGAAGG+TGG - chr1.3:67786899-67786918 MS.gene59225:intron 55.0%
TAGGTCTAGGGAACGCAAGC+AGG - chr1.3:67787018-67787037 MS.gene59225:intron 55.0%
TGTGAAAGCTGGTGCGGTTG+AGG - chr1.3:67786718-67786737 MS.gene59225:intron 55.0%
AGCTGGTGCGGTTGAGGATG+AGG - chr1.3:67786724-67786743 MS.gene59225:intron 60.0%
ATCGTCGACGGTTCGAGGAG+TGG + chr1.3:67786603-67786622 None:intergenic 60.0%
ATGGTTGAAGGTGGGGGGAG+GGG - chr1.3:67786908-67786927 MS.gene59225:intron 60.0%
CAGACATCGTCGACGGTTCG+AGG + chr1.3:67786608-67786627 None:intergenic 60.0%
CCGTTATCGGAGACGCTCAG+TGG - chr1.3:67786776-67786795 MS.gene59225:intron 60.0%
CGATGTGATCTCCGGCGACA+GGG - chr1.3:67786646-67786665 MS.gene59225:intron 60.0%
CTCGAACCGTCGACGATGTC+TGG - chr1.3:67786606-67786625 MS.gene59225:intron 60.0%
CTCTCGCCAGACATCGTCGA+CGG + chr1.3:67786615-67786634 None:intergenic 60.0%
CTTGGCGGTGAAGCTAGAGG+AGG - chr1.3:67787299-67787318 MS.gene59225:intron 60.0%
GCGGTTGAGGATGAGGAGGA+GGG - chr1.3:67786731-67786750 MS.gene59225:intron 60.0%
GTCCTTGGCGGTGAAGCTAG+AGG - chr1.3:67787296-67787315 MS.gene59225:intron 60.0%
TCGGAGACGCTCAGTGGTAG+TGG - chr1.3:67786782-67786801 MS.gene59225:intron 60.0%
TGAGGATGAGGAGGAGGGTG+AGG - chr1.3:67786736-67786755 MS.gene59225:intron 60.0%
TGCGGTTGAGGATGAGGAGG+AGG - chr1.3:67786730-67786749 MS.gene59225:intron 60.0%
TGGTGCGGTTGAGGATGAGG+AGG - chr1.3:67786727-67786746 MS.gene59225:intron 60.0%
! CTCCTCTAGCTTCACCGCCA+AGG + chr1.3:67787301-67787320 None:intergenic 60.0%
GACGGTTCGAGGAGTGGCGA+CGG + chr1.3:67786597-67786616 None:intergenic 65.0%
GCGATGTGATCTCCGGCGAC+AGG - chr1.3:67786645-67786664 MS.gene59225:intron 65.0%
GTCGCCGGAGATCACATCGC+CGG + chr1.3:67786645-67786664 None:intergenic 65.0%
GTCGCCGGCGATGTGATCTC+CGG - chr1.3:67786638-67786657 MS.gene59225:intron 65.0%
GTCTGGCGAGAGATCGTCGC+CGG - chr1.3:67786623-67786642 MS.gene59225:intron 65.0%
TGGTTGAAGGTGGGGGGAGG+GGG - chr1.3:67786909-67786928 MS.gene59225:intron 65.0%
GGGGGAGGGGGAAGAGAGGA+CGG - chr1.3:67786921-67786940 MS.gene59225:intron 70.0%
TCGAGGAGTGGCGACGGCGT+CGG + chr1.3:67786591-67786610 None:intergenic 70.0%
GGTGGGGGGAGGGGGAAGAG+AGG - chr1.3:67786917-67786936 MS.gene59225:intron 75.0%
!! AGTGGCGACGGCGTCGGCGA+GGG + chr1.3:67786585-67786604 None:intergenic 75.0%
!!! GAGTGGCGACGGCGTCGGCG+AGG + chr1.3:67786586-67786605 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 67786485 67788305 67786485 ID=MS.gene59225
chr1.3 mRNA 67786485 67788305 67786485 ID=MS.gene59225.t1;Parent=MS.gene59225
chr1.3 exon 67787751 67788305 67787751 ID=MS.gene59225.t1.exon1;Parent=MS.gene59225.t1
chr1.3 CDS 67787751 67788305 67787751 ID=cds.MS.gene59225.t1;Parent=MS.gene59225.t1
chr1.3 exon 67787438 67787514 67787438 ID=MS.gene59225.t1.exon2;Parent=MS.gene59225.t1
chr1.3 CDS 67787438 67787514 67787438 ID=cds.MS.gene59225.t1;Parent=MS.gene59225.t1
chr1.3 exon 67786485 67786521 67786485 ID=MS.gene59225.t1.exon3;Parent=MS.gene59225.t1
chr1.3 CDS 67786485 67786521 67786485 ID=cds.MS.gene59225.t1;Parent=MS.gene59225.t1
Gene Sequence

>MS.gene59225

ATGGCTTCTTCCAAGCTCATGCCTTCTTCCGATCCACCTTCTTCTCCATCTCCTAAACCTCACTTCACCACCGCCAATTTCCTCGACGATCAAATCTCCCTCGCCGACGCCGTCGCCACTCCTCGAACCGTCGACGATGTCTGGCGAGAGATCGTCGCCGGCGATGTGATCTCCGGCGACAGGGAGTGTAAAGAAGAGATTTCCGATGAGATGATGACGTTGGAGGATTTTCTTGTGAAAGCTGGTGCGGTTGAGGATGAGGAGGAGGGTGAGGATGTGAAGATGACGATTCCGTTATCGGAGACGCTCAGTGGTAGTGGTATGTTTTCGTTGGATTCGAGTTTTCAAGGAATTGAAAACGTTGATGGGTCGGTGATAGGGTTTGGGAATGGGAATGGCAATGGGAATGTGAATGGGGTGGAAATGGTTGAAGGTGGGGGGAGGGGGAAGAGAGGACGGCCTGTTATGGAGCAATTGGATAAAGCTGCTCAGCAGAGACAGAGGAGAATGATTAAGAATAGAGAATCTGCTGCTAGGTCTAGGGAACGCAAGCAGGTATTGATTGTTTTTGTGTCTGATATGTTATGATACACGGACACCGGACATGACACTGACACGAGTAATAATTTGAGAAACTGATTGTAATCAAATGTGTCAGTGTTGTGTTGGACTAAAAACACGTGTCGGATACAGGACTCGTCTTTGCTCAGATGTTTTAGTGCTACATGATATGTGCTTTTGTTTTTGTGATGTGGGTTTTTGACTTTTGTTTTGGTTTTGTGTGATTTTAGGCTTATCAAGTGGAATTGGAGTCCTTGGCGGTGAAGCTAGAGGAGGAAAATGACAAGTTGATGAAGGAAAAGGTGATGTATACTTGCATTTTCCGCAATTTTTTTATAAATCAGCAATGAATTGCGATTTTGATCATCAAAATTGTAGAAATTGTGCTATTTATTATAGTAAATTTCCGAAATGGTAAAACCGTCTTTGGAATTGATGATGGTCTGTGGATTTAATGAATATCTCTATTTAAACTTTGTTGTCGGTAGGATTTAAAGTAGGGTTAGGGTGCAATTAAGAACTAAGAAGAGATTGCTGTCAATTAATTTGGATTCCTCTATTGATGATAGTTTAGAAAGTGATGATTTTTCACGACAGAGGGACAAAGTTGTTTGGCAGTTTTCGATTTAAGGGATAATTTTGACATTTCATAAATTAGGGTCTATATTGTCTGATGCTTACGATTTTGAAGACTAAAACGATGATTAATTCATTCCACATTTTCCATCAGCTGCTTGCTTTTTGCTGTGGTTAATGCTACAAGGCAAACATTCTTTGTGGTCTATGCTGAACTTGAACCATTCGACTCTGATTAAGGGACTAAATTATAATTAGTGCACTCAAGTTGTGCTTGTTAACAGAAGTGGTGAACCAATGTCCATTATTGAAATCCTTCTGTTTTTTTTTTCAAACAACGAAATCAATGTTTTCGCCTTTGACTGTGCAATTAGTTTTTACGTGGGTAATTTCCATAACGGCCTCATAGAATTCAGTCCTAAAATCTCATTCTATTTGTGTCTGAGCCACGAAGTTCCTTTTTTGTTCTCCTAACACATGCTACCATGCTTGTTTTTATATATTTGGAATGTTTCTTGGAGCACTGTTGGTTACAACTTCTGTCTCTCTCAGTCTCATACTCCTGCAACCCTTTGAATTTTCAGATTTAGAGTTTATTAATGATTATGCTTTCCCCCTGACATCTAACAGATAATATTCTTGTGCAGGCTGAGAGGAAAAAGGAGAGGTTCAAGCAGGTACTGA
Protein sequence

>MS.gene59225.t1

MASSKLMPSSDPPSSPSPKPHFTTANFLDDQISLADAVATPRTVDDVWREIVAGDVISGDRECKEEISDEMMTLEDFLVKAGAVEDEEEGEDVKMTIPLSETLSGSGMFSLDSSFQGIENVDGSVIGFGNGNGNGNVNGVEMVEGGGRGKRGRPVMEQLDKAAQQRQRRMIKNRESAARSRERKQAYQVELESLAVKLEEENDKLMKEKVMLRGKRRGSSRY